SitesBLAST
Comparing 6938704 FitnessBrowser__SB2B:6938704 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P33221 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; GAR transformylase T; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Escherichia coli (strain K12) (see 5 papers)
69% identity, 99% coverage: 1:390/392 of query aligns to 3:391/392 of P33221
- EL 22:23 (= EL 20:21) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- E82 (= E80) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- R114 (= R112) binding ATP
- K155 (= K153) binding ATP
- SSGKGQ 160:165 (= SSGKGQ 158:163) binding ATP
- G162 (= G160) mutation to I: Strong decrease in the reaction rate for the conversion of formate to FGAR and in the affinity for formate. 3- and 2-fold decrease in the affinity for ATP and GAR, respectively.
- K179 (≠ D177) modified: N6-acetyllysine
- EGVV 195:198 (≠ EGFI 193:196) binding ATP
- E203 (= E201) binding ATP
- E267 (= E265) binding Mg(2+)
- E279 (= E277) binding Mg(2+)
- D286 (= D284) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- K355 (= K354) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- RR 362:363 (= RR 361:362) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
1kjiA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-amppcp (see paper)
69% identity, 99% coverage: 1:390/392 of query aligns to 2:388/389 of 1kjiA
- active site: E114 (= E113), K154 (= K153), S159 (= S158), G161 (= G160), E264 (= E265), E276 (= E277), D283 (= D284), T284 (= T285), R360 (= R362)
- binding phosphomethylphosphonic acid adenylate ester: R113 (= R112), I152 (≠ V151), K154 (= K153), S159 (= S158), S160 (= S159), G161 (= G160), Q164 (= Q163), E192 (= E193), V195 (≠ I196), E200 (= E201), Q222 (= Q223), E264 (= E265), F266 (= F267), E276 (= E277)
- binding magnesium ion: E264 (= E265), E276 (= E277)
1ez1A Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg, amppnp, and gar (see paper)
69% identity, 99% coverage: 1:390/392 of query aligns to 2:388/389 of 1ez1A
- active site: E114 (= E113), K154 (= K153), S159 (= S158), G161 (= G160), E264 (= E265), E276 (= E277), D283 (= D284), T284 (= T285), R360 (= R362)
- binding phosphoaminophosphonic acid-adenylate ester: R113 (= R112), I152 (≠ V151), K154 (= K153), S159 (= S158), S160 (= S159), G161 (= G160), E192 (= E193), V194 (≠ F195), V195 (≠ I196), F197 (= F198), E200 (= E201), Q222 (= Q223), E264 (= E265), F266 (= F267), E276 (= E277)
- binding glycinamide ribonucleotide: G20 (= G19), E21 (= E20), L22 (= L21), E81 (= E80), I82 (= I81), S160 (= S159), D283 (= D284), K352 (= K354), R359 (= R361), R360 (= R362)
- binding magnesium ion: E264 (= E265), E276 (= E277)
1eyzA Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg and amppnp (see paper)
69% identity, 99% coverage: 1:390/392 of query aligns to 2:388/389 of 1eyzA
- active site: E114 (= E113), K154 (= K153), S159 (= S158), G161 (= G160), E264 (= E265), E276 (= E277), D283 (= D284), T284 (= T285), R360 (= R362)
- binding phosphoaminophosphonic acid-adenylate ester: R113 (= R112), I152 (≠ V151), K154 (= K153), S159 (= S158), S160 (= S159), G161 (= G160), Q164 (= Q163), E192 (= E193), V195 (≠ I196), F197 (= F198), E200 (= E201), E264 (= E265), F266 (= F267), E276 (= E277)
- binding magnesium ion: E264 (= E265), E276 (= E277)
1kjqA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-adp (see paper)
68% identity, 99% coverage: 1:390/392 of query aligns to 2:387/388 of 1kjqA
- active site: E114 (= E113), K154 (= K153), E263 (= E265), E275 (= E277), D282 (= D284), T283 (= T285), R359 (= R362)
- binding adenosine-5'-diphosphate: R113 (= R112), I152 (≠ V151), K154 (= K153), E191 (= E193), V193 (≠ F195), V194 (≠ I196), F196 (= F198), E199 (= E201), Q221 (= Q223), F265 (= F267), E275 (= E277)
- binding magnesium ion: E263 (= E265), E275 (= E277)
1kjjA Crystal structure of glycniamide ribonucleotide transformylase in complex with mg-atp-gamma-s (see paper)
68% identity, 99% coverage: 1:390/392 of query aligns to 2:385/386 of 1kjjA
- active site: E114 (= E113), K154 (= K153), S159 (= S158), G161 (= G160), E261 (= E265), E273 (= E277), D280 (= D284), T281 (= T285), R357 (= R362)
- binding phosphothiophosphoric acid-adenylate ester: R113 (= R112), I152 (≠ V151), K154 (= K153), S159 (= S158), S160 (= S159), G161 (= G160), Q164 (= Q163), E189 (= E193), V192 (≠ I196), E197 (= E201), Q219 (= Q223), E261 (= E265), F263 (= F267), E273 (= E277)
- binding magnesium ion: E261 (= E265), E273 (= E277)
1kj8A Crystal structure of purt-encoded glycinamide ribonucleotide transformylase in complex with mg-atp and gar (see paper)
68% identity, 99% coverage: 1:390/392 of query aligns to 2:385/386 of 1kj8A
- active site: E114 (= E113), K154 (= K153), S159 (= S158), G161 (= G160), E261 (= E265), E273 (= E277), D280 (= D284), T281 (= T285), R357 (= R362)
- binding adenosine-5'-triphosphate: R113 (= R112), I152 (≠ V151), K154 (= K153), S159 (= S158), S160 (= S159), G161 (= G160), Q164 (= Q163), E189 (= E193), V192 (≠ I196), F194 (= F198), E197 (= E201), Q219 (= Q223), G222 (= G226), E261 (= E265), F263 (= F267), E273 (= E277)
- binding glycinamide ribonucleotide: G20 (= G19), E21 (= E20), L22 (= L21), E81 (= E80), I82 (= I81), S160 (= S159), D280 (= D284), K349 (= K354), R356 (= R361)
- binding magnesium ion: E261 (= E265), E273 (= E277)
O58056 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
48% identity, 99% coverage: 2:389/392 of query aligns to 8:410/430 of O58056
2dwcB Crystal structure of probable phosphoribosylglycinamide formyl transferase from pyrococcus horikoshii ot3 complexed with adp
47% identity, 99% coverage: 2:389/392 of query aligns to 10:399/409 of 2dwcB
- active site: E265 (= E265), E277 (= E277), D284 (= D284), T285 (= T285), R372 (= R362)
- binding adenosine-5'-diphosphate: R120 (= R112), H159 (≠ V151), K161 (= K153), H190 (≠ F195), I191 (= I196), F193 (= F198), E196 (= E201), F267 (= F267), E277 (= E277)
3ax6A Crystal structure of n5-carboxyaminoimidazole ribonucleotide synthetase from thermotoga maritima
26% identity, 93% coverage: 25:389/392 of query aligns to 16:357/360 of 3ax6A
- active site: E231 (= E265), E244 (= E277), N251 (≠ D284), S252 (≠ T285), K330 (≠ R362)
- binding adenosine-5'-diphosphate: K101 (≠ R112), V136 (= V151), K138 (= K153), E164 (= E193), F166 (= F195), V167 (≠ I196), E172 (= E201), F233 (= F267), N243 (≠ S276)
4mamA The crystal structure of phosphoribosylaminoimidazole carboxylase atpase subunit of francisella tularensis subsp. Tularensis schu s4 in complex with an adp analog, amp-cp
30% identity, 77% coverage: 15:316/392 of query aligns to 5:298/373 of 4mamA
- active site: Y144 (≠ S158), G146 (= G160), E247 (= E265), E259 (= E277), N266 (≠ D284), S267 (≠ T285)
- binding phosphomethylphosphonic acid adenosyl ester: R98 (= R112), I136 (≠ V151), K138 (= K153), Y144 (≠ S158), G146 (= G160), Q149 (= Q163), E175 (= E193), F177 (= F195), V178 (≠ I196), F180 (= F198), E183 (= E201), H206 (≠ Q223), F249 (= F267), E259 (= E277)
Sites not aligning to the query:
4ma0A The crystal structure of phosphoribosylaminoimidazole carboxylase atpase subunit of francisella tularensis subsp. Tularensis schu s4 in complex with partially hydrolysed atp
30% identity, 77% coverage: 15:316/392 of query aligns to 5:298/366 of 4ma0A