SitesBLAST
Comparing 6938906 Sama_3009 succinylglutamic semialdehyde dehydrogenase (RefSeq) to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5u0mA Fatty aldehyde dehydrogenase from marinobacter aquaeolei vt8 and cofactor complex (see paper)
64% identity, 99% coverage: 4:486/486 of query aligns to 7:488/488 of 5u0mA
- active site: N148 (= N145), K171 (= K168), E246 (= E243), C280 (= C277), E377 (= E375), P455 (≠ A453)
- binding nicotinamide-adenine-dinucleotide: F144 (= F141), Y147 (= Y144), N148 (= N145), K171 (= K168), S173 (= S170), E174 (= E171), G207 (= G204), T222 (= T219), G223 (= G220), S224 (= S221), V227 (≠ T224), E246 (= E243), M247 (= M244), G248 (= G245), C280 (= C277), E377 (= E375), F379 (= F377)
5u0lA X-ray crystal structure of fatty aldehyde dehydrogenase enzymes from marinobacter aquaeolei vt8 complexed with a substrate (see paper)
64% identity, 99% coverage: 4:486/486 of query aligns to 7:488/488 of 5u0lA
- active site: N148 (= N145), K171 (= K168), E246 (= E243), C280 (= C277), E377 (= E375), P455 (≠ A453)
- binding decanal: K107 (= K104), H152 (= H149), L153 (= L150), G156 (= G153), H157 (= H154), S456 (= S454), A457 (= A455)
3ju8A Crystal structure of succinylglutamic semialdehyde dehydrogenase from pseudomonas aeruginosa.
61% identity, 99% coverage: 2:484/486 of query aligns to 4:486/486 of 3ju8A
- active site: N147 (= N145), K170 (= K168), E245 (= E243), C279 (= C277), E377 (= E375), P455 (≠ A453)
- binding nicotinamide-adenine-dinucleotide: G144 (= G142), Y146 (= Y144), N147 (= N145), L152 (= L150), K170 (= K168), S172 (= S170), F220 (= F218), T221 (= T219), G222 (= G220), S223 (= S221), T226 (= T224), E245 (= E243), M246 (= M244), G247 (= G245), C279 (= C277), E377 (= E375), F379 (= F377), F444 (= F442)
5izdA Wild-type glyceraldehyde dehydrogenase from thermoplasma acidophilum in complex with NADP
31% identity, 84% coverage: 4:411/486 of query aligns to 6:417/494 of 5izdA
- active site: N149 (= N145), K172 (= K168), E247 (= E243), C281 (= C277), E381 (= E375)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: L145 (≠ F141), T146 (≠ G142), W148 (≠ Y144), K172 (= K168), P173 (= P169), S174 (= S170), S175 (≠ E171), R204 (≠ E200), G205 (vs. gap), G209 (= G204), D210 (≠ K205), G225 (= G220), S226 (= S221), T229 (= T224)
Sites not aligning to the query:
P25526 Succinate-semialdehyde dehydrogenase [NADP(+)] GabD; SSDH; Glutarate-semialdehyde dehydrogenase; EC 1.2.1.79; EC 1.2.1.- from Escherichia coli (strain K12) (see paper)
33% identity, 94% coverage: 4:461/486 of query aligns to 14:472/482 of P25526
Q9H2A2 2-aminomuconic semialdehyde dehydrogenase; Aldehyde dehydrogenase 12; Aldehyde dehydrogenase family 8 member A1; EC 1.2.1.32 from Homo sapiens (Human) (see paper)
31% identity, 95% coverage: 1:461/486 of query aligns to 10:476/487 of Q9H2A2
- R109 (≠ L107) mutation to A: About 65-fold loss of catalytic efficiency.
- N155 (= N145) mutation to A: Complete loss of activity.
- R451 (≠ A436) mutation to A: Complete loss of activity.
Q56YU0 Aldehyde dehydrogenase family 2 member C4; ALDH1a; Protein REDUCED EPIDERMAL FLUORESCENCE 1; EC 1.2.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
32% identity, 99% coverage: 4:482/486 of query aligns to 23:499/501 of Q56YU0
- G152 (= G126) mutation to E: In ref1-7; reduced activity on sinapaldehyde.
- G416 (≠ A392) mutation to R: In ref1-6; reduced activity on sinapaldehyde.
3jz4A Crystal structure of e. Coli NADP dependent enzyme (see paper)
33% identity, 94% coverage: 4:461/486 of query aligns to 13:471/481 of 3jz4A
- active site: N156 (= N145), K179 (= K168), E254 (= E243), C288 (= C277), E385 (= E375), E462 (≠ A453)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: P154 (= P143), W155 (≠ Y144), K179 (= K168), A181 (≠ S170), S182 (≠ E171), A212 (≠ V201), G216 (= G204), G232 (= G220), S233 (= S221), I236 (≠ T224), C288 (= C277), K338 (≠ G329), E385 (= E375), F387 (= F377)
Q9HTJ1 NAD/NADP-dependent betaine aldehyde dehydrogenase; BADH; EC 1.2.1.8 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 2 papers)
31% identity, 92% coverage: 4:452/486 of query aligns to 10:463/490 of Q9HTJ1
- GAWN 150:153 (≠ GPYN 142:145) binding
- K162 (≠ H154) active site, Charge relay system
- KPSE 176:179 (= KPSE 168:171) binding
- G209 (vs. gap) binding
- GTST 230:233 (≠ SSRT 221:224) binding
- E252 (= E243) active site, Proton acceptor
- C286 (= C277) binding covalent; modified: Cysteine sulfenic acid (-SOH)
- E387 (= E375) binding
Sites not aligning to the query:
- 464 active site, Charge relay system
4cazA Crystal structure of betaine aldehyde dehydrogenase from pseudomonas aeruginosa in complex with nadh
31% identity, 92% coverage: 4:452/486 of query aligns to 9:462/489 of 4cazA
- active site: N152 (= N145), K175 (= K168), E251 (= E243), C285 (= C277), E386 (= E375)
- binding [[(2R,3S,4R,5R)-5-[(3R)-3-aminocarbonyl-3,4-dihydro-2H-pyridin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanidyl-phosphoryl] [(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl phosphate: I148 (≠ F141), G149 (= G142), W151 (≠ Y144), N152 (= N145), K175 (= K168), E178 (= E171), G208 (vs. gap), G212 (= G204), F226 (= F218), T227 (= T219), G228 (= G220), G229 (≠ S221), T232 (= T224), V236 (≠ L228), E251 (= E243), L252 (≠ M244), C285 (= C277), E386 (= E375), F388 (= F377)
Sites not aligning to the query:
2woxA Betaine aldehyde dehydrogenase from pseudomonas aeruginosa with NAD(p) h-catalytic thiol adduct. (see paper)
31% identity, 92% coverage: 4:452/486 of query aligns to 9:462/489 of 2woxA
- active site: N152 (= N145), K175 (= K168), E251 (= E243), C285 (= C277), E386 (= E375)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I148 (≠ F141), G149 (= G142), W151 (≠ Y144), N152 (= N145), K175 (= K168), S177 (= S170), E178 (= E171), G208 (vs. gap), G212 (= G204), F226 (= F218), T227 (= T219), G228 (= G220), G229 (≠ S221), T232 (= T224), V236 (≠ L228), E251 (= E243), L252 (≠ M244), C285 (= C277), E386 (= E375), F388 (= F377)
Sites not aligning to the query:
2wmeA Crystallographic structure of betaine aldehyde dehydrogenase from pseudomonas aeruginosa (see paper)
31% identity, 92% coverage: 4:452/486 of query aligns to 9:462/489 of 2wmeA
- active site: N152 (= N145), K175 (= K168), E251 (= E243), C285 (= C277), E386 (= E375)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G149 (= G142), W151 (≠ Y144), K175 (= K168), S177 (= S170), E178 (= E171), G208 (vs. gap), G212 (= G204), F226 (= F218), G228 (= G220), G229 (≠ S221), T232 (= T224), V236 (≠ L228)
Sites not aligning to the query:
3ty7B Crystal structure of aldehyde dehydrogenase family protein from staphylococcus aureus
31% identity, 88% coverage: 3:431/486 of query aligns to 8:436/454 of 3ty7B
1t90A Crystal structure of methylmalonate semialdehyde dehydrogenase from bacillus subtilis
29% identity, 92% coverage: 1:448/486 of query aligns to 5:453/484 of 1t90A
- active site: N151 (= N145), K174 (= K168), L248 (= L242), C282 (= C277), E380 (= E375)
- binding nicotinamide-adenine-dinucleotide: I147 (≠ F141), A148 (≠ G142), P149 (= P143), F150 (≠ Y144), N151 (= N145), W159 (≠ G153), K174 (= K168), E177 (= E171), R178 (≠ L172), H207 (≠ V201), V225 (≠ T219), G226 (= G220), S227 (= S221), V230 (≠ T224), L248 (= L242), T249 (≠ M244), C282 (= C277), E380 (= E375), F382 (= F377)
Sites not aligning to the query:
P42412 Malonate-semialdehyde dehydrogenase; MSA dehydrogenase; Methylmalonate-semialdehyde dehydrogenase; MMSA dehydrogenase; MMSDH; MSDH; EC 1.2.1.27 from Bacillus subtilis (strain 168) (see 3 papers)
29% identity, 92% coverage: 1:448/486 of query aligns to 7:455/487 of P42412
- C36 (≠ W29) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-160; A-287; A-351 and A-413.
- R107 (≠ A100) mutation to L: At least 50-fold decrease of the second-order rate constant for the acylation step.
- A150 (≠ G142) binding
- F152 (≠ Y144) binding
- C160 (≠ N152) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-287; A-351 and A-413.
- K176 (= K168) binding
- E179 (= E171) binding
- R180 (≠ L172) binding
- S229 (= S221) binding
- T251 (≠ M244) binding
- R283 (= R276) mutation to L: At least 50-fold decrease of the second-order rate constant for the acylation step.
- C287 (≠ A280) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-351 and A-413.
- C351 (≠ V346) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-287 and A-413.
- E382 (= E375) binding
- C413 (≠ G406) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-287 and A-351.
4pz2B Structure of zm aldh2-6 (rf2f) in complex with NAD (see paper)
31% identity, 99% coverage: 4:482/486 of query aligns to 14:492/494 of 4pz2B
- active site: N159 (= N145), K182 (= K168), E258 (= E243), C292 (= C277), E392 (= E375), D469 (≠ A453)
- binding nicotinamide-adenine-dinucleotide: I155 (≠ F141), I156 (≠ G142), P157 (= P143), W158 (≠ Y144), N159 (= N145), M164 (≠ L150), K182 (= K168), A184 (≠ S170), E185 (= E171), G215 (≠ V201), G219 (= G204), F233 (= F218), T234 (= T219), G235 (= G220), S236 (= S221), V239 (≠ T224), E258 (= E243), L259 (≠ M244), C292 (= C277), E392 (= E375), F394 (= F377)
7radA Crystal structure analysis of aldh1b1
31% identity, 92% coverage: 4:452/486 of query aligns to 16:468/493 of 7radA
- binding nicotinamide-adenine-dinucleotide: I158 (≠ F141), I159 (≠ G142), P160 (= P143), W161 (≠ Y144), N162 (= N145), M167 (≠ L150), K185 (= K168), E188 (= E171), G218 (≠ V201), G222 (= G204), A223 (≠ K205), T237 (= T219), G238 (= G220), S239 (= S221), V242 (≠ T224), E261 (= E243), L262 (≠ M244), C295 (= C277), E392 (= E375), F394 (= F377)
- binding 3-(2-methoxyphenyl)-1-(4-phenylphenyl)-6,7,8,9-tetrahydro-5~{H}-imidazo[1,2-a][1,3]diazepine: L113 (≠ E97), E117 (≠ M101), F163 (= F146), E285 (≠ Q267), F289 (≠ I271), N450 (≠ T434), V452 (≠ A436)
8vr1A Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (ctp bound)
31% identity, 92% coverage: 4:452/486 of query aligns to 8:461/488 of 8vr1A
8vr0A Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (gmp bound)
31% identity, 92% coverage: 4:452/486 of query aligns to 8:461/488 of 8vr0A
8vqzA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (cmp bound)
31% identity, 92% coverage: 4:452/486 of query aligns to 8:461/488 of 8vqzA
Query Sequence
>6938906 Sama_3009 succinylglutamic semialdehyde dehydrogenase (RefSeq)
MTQFINGQWLAGEGKEMQSKNPANGEVIWQGKAAVPAQVQAAVMAARDAQFEWFMLGFEG
RQAIVEAYRNELEANKAELAEVIAQETGKPRWETATEAAAMIGKIGLSVSAYHKRTGTEV
NEGAAGRAVLRHKPHGVVAVFGPYNFPGHLPNGHIVPALLAGNTVVFKPSELTPKVAELM
LKLWEKAGLPAGVINLVQGEVETGKALASHPQLDGLFFTGSSRTGHLLHQQYAGHPGKIL
ALEMGGNNPLIVKGVSDTRAAIHDIIQSAFISSGQRCTCARRLYVEKGAEGDKLLAGLVE
AVKAIKVGPWNADPQPFMGSMISETAAKGMLDAQRNLLNLGAKSLVEMTHLQAGTGLVSP
GLIDVTEVIELPDEEYFGPLLQVVRYTSFDEAIRLANDTRYGLSAGILADDKADYEYFLA
RIRAGIVNWNKQITGASGAAPFGGVGASGNHRASAFYAADYCAYPVASMEADSVTLPASL
SPGLTL
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory