SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 6939068 FitnessBrowser__SB2B:6939068 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

Q6LX42 Sodium/alanine symporter AgcS; Alanine permease; Alanine/glycine:cation symporter from Methanococcus maripaludis (strain S2 / LL) (see paper)
50% identity, 98% coverage: 6:455/459 of query aligns to 5:453/453 of Q6LX42

query
sites
Q6LX42

E

S

L

L

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N

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A

T75 (= T79) binding ; binding
G79 (= G83) binding
N80 (= N84) binding ; mutation to A: Markedly reduces L-alanine uptake; when associated with A-274 and A-308.
I165 (= I167) mutation to A: Shows poor substrate selectivity, allowing additional amino acids such as L-valine and L-leucine to be efficiently transported. Decreases L-alanine uptake.
Q170 (= Q172) binding ; binding
F273 (= F279) mutation to A: Shows poor substrate selectivity, allowing additional amino acids such as L-valine and L-leucine to be efficiently transported. Decreases L-alanine uptake.
FS 273:274 (= FS 279:280) binding
FSNE 273:276 (= FSNE 279:282) binding
S274 (= S280) mutation to A: Markedly reduces L-alanine uptake; when associated with A-80 and A-308.
D308 (= D314) mutation to A: Markedly reduces L-alanine uptake; when associated with A-80 and A-274.
W370 (= W372) mutation to Q: Does not change uptake of L-alanine, but reduces the ability to transport D-alanine.

6csfM Crystal structure of sodium/alanine symporter agcs with d-alanine bound (see paper)
51% identity, 95% coverage: 16:452/459 of query aligns to 1:430/431 of 6csfM

query
sites
6csfM

W

W

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A

binding d-alanine: T58 (= T79), G60 (= G81), G62 (= G83), N63 (= N84), Q150 (= Q172), F253 (= F279), S254 (= S280)
binding sodium ion: T58 (= T79), I59 (= I80), N63 (= N84), S254 (= S280), D288 (= D314)

6cseM Crystal structure of sodium/alanine symporter agcs with l-alanine bound (see paper)
51% identity, 95% coverage: 16:452/459 of query aligns to 1:430/431 of 6cseM

query
sites
6cseM

W

W

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S

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L

M

P

P

I

M

G

G

D

G

Y

L

I

I

V

V

A

T

V

I

A

G

L

L

A

V

I

F

F

F

A

A

F

Y

T

S

T

T

I

V

L

L

G

G

W

W

S

S

V

Y

Y

Y

S

G

E

E

K

K

C

C

V

F

Q

E

Y

Y

L

L

L

I

G

G

V

T

K

K

A

G

V

I

M

R

P

L

F

Y

R

R

L

I

I

A

W

F

T

V

L

L

V

V

V

A

P

F

L

W

G

G

A

A

M

T

G

A

S

S

L

L

D

P

F

L

V

V

W

W

L

N

L

I

A

A

D

D

T

T

L

L

N

N

A

G

M

A

M

M

A

A

I

I

P

P

N

N

L

L

I

I

A

G

L

L

A

L

L

L

L

L

S

S

P

G

V

V

V

V

F

V

K

S

L

E

T

T

R

K

E

A

F

F

F

N

S

E

S

I

R

R

D

K

E

N

A

E

S

A

binding alanine: A57 (= A78), T58 (= T79), G60 (= G81), N63 (= N84), Q150 (= Q172), F253 (= F279), S254 (= S280), E256 (= E282)
binding sodium ion: T58 (= T79), I59 (= I80), N63 (= N84), S254 (= S280), D288 (= D314)

Query Sequence

>6939068 FitnessBrowser__SB2B:6939068
MEAVTELVKSLNSLVWGIPMLVMILGVGLYLSLGLKLLPILKLGKGFRLLWSGRTPGQGK
EVKGDVSPFNALMTSLSATIGTGNIAGVATAIFVGGPGALFWMWCTALVGMATKYSEAVL
AVRYREVDEKGNYIGGPMYYIKNGLGSKWTWLGTAFALFGSFAGFGIGNTVQANSVASAL
TSNFGVPAWVSGVVMMVLVGLVLMGGIKRIAIVAGKLVPLMTIFYITAGIAVLVVYASEI
PAALSLIISSAFSPVAAQGGFAGAAVMVAIQMGVARGVFSNEAGLGSAPIAHAAAQTKSP
VTQGLVGMLGTFIDTIIVCSITGLAIVVSGAWTSGENGAALTSYAFSHALPIGDYIVAVA
LAIFAFTTILGWSVYSEKCVQYLLGVKAVMPFRLIWTLVVPLGAMGSLDFVWLLADTLNA
MMAIPNLIALALLSPVVFKLTREFFSSRDEASTKSVEAE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory