SitesBLAST
Comparing 6939345 FitnessBrowser__SB2B:6939345 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
8gy3B Cryo-em structure of membrane-bound aldehyde dehydrogenase from gluconobacter oxydans
51% identity, 97% coverage: 2:148/151 of query aligns to 3:152/154 of 8gy3B
- binding fe2/s2 (inorganic) cluster: G38 (= G37), C39 (= C38), G40 (= G39), G42 (= G41), C44 (= C43), G45 (= G44), C47 (= C46), C59 (= C58), C97 (= C93), C100 (= C96), Q101 (= Q97), C132 (= C128), C134 (= C130)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q96 (= Q92), C134 (= C130)
1t3qA Crystal structure of quinoline 2-oxidoreductase from pseudomonas putida 86 (see paper)
47% identity, 93% coverage: 3:142/151 of query aligns to 7:150/162 of 1t3qA
- binding fe2/s2 (inorganic) cluster: I40 (≠ Y36), C42 (= C38), E43 (≠ G39), G45 (= G41), C47 (= C43), G48 (= G44), C50 (= C46), R60 (= R56), C62 (= C58), C101 (= C93), G102 (= G94), C104 (= C96), C136 (= C128), C138 (= C130)
- binding pterin cytosine dinucleotide: Q100 (= Q92), C138 (= C130)
5y6qA Crystal structure of an aldehyde oxidase from methylobacillus sp. Ky4400 (see paper)
44% identity, 92% coverage: 3:141/151 of query aligns to 6:149/157 of 5y6qA
- binding fe2/s2 (inorganic) cluster: G40 (= G37), C41 (= C38), D42 (≠ G39), G44 (= G41), C46 (= C43), G47 (= G44), C49 (= C46), C61 (= C58), C101 (= C93), G102 (= G94), C104 (= C96), C136 (= C128), C138 (= C130)
- binding pterin cytosine dinucleotide: Q100 (= Q92), C138 (= C130)
5g5gA Escherichia coli periplasmic aldehyde oxidase (see paper)
39% identity, 95% coverage: 3:146/151 of query aligns to 13:175/175 of 5g5gA
- binding fe2/s2 (inorganic) cluster: G47 (= G37), C48 (= C38), D49 (≠ G39), G51 (= G41), C53 (= C43), G54 (= G44), C56 (= C46), C68 (= C58), C107 (= C93), G108 (= G94), C110 (= C96), C157 (= C128), C159 (= C130)
P77165 Aldehyde oxidoreductase iron-sulfur-binding subunit PaoA; EC 1.2.99.6 from Escherichia coli (strain K12) (see paper)
39% identity, 95% coverage: 3:146/151 of query aligns to 64:226/229 of P77165
- C99 (= C38) binding
- C104 (= C43) binding
- G105 (= G44) binding
- C107 (= C46) binding
- C119 (= C58) binding
- C158 (= C93) binding
- C161 (= C96) binding
- C208 (= C128) binding
- C210 (= C130) binding
1sb3C Structure of 4-hydroxybenzoyl-coa reductase from thauera aromatica (see paper)
43% identity, 91% coverage: 4:141/151 of query aligns to 7:148/161 of 1sb3C
- binding fe2/s2 (inorganic) cluster: Q39 (≠ Y36), C41 (= C38), G44 (= G41), C46 (= C43), G47 (= G44), C49 (= C46), C61 (= C58), C100 (= C93), G101 (= G94), C103 (= C96), C135 (= C128), C137 (= C130)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q92), C137 (= C130)
3hrdD Crystal structure of nicotinate dehydrogenase (see paper)
37% identity, 92% coverage: 3:141/151 of query aligns to 7:149/160 of 3hrdD
- binding flavin-adenine dinucleotide: E44 (≠ A40), G45 (= G41), E46 (≠ L42)
- binding fe2/s2 (inorganic) cluster: E40 (≠ Y36), C42 (= C38), S43 (≠ G39), G45 (= G41), C47 (= C43), G48 (= G44), C50 (= C46), C62 (= C58), Q100 (= Q92), C101 (= C93), G102 (= G94), C104 (= C96), C136 (= C128), C138 (= C130)
- binding pterin cytosine dinucleotide: Q100 (= Q92), C138 (= C130)
Q0QLF3 Nicotinate dehydrogenase small FeS subunit; NDH; Nicotinic acid hydroxylase small FeS subunit; NAH; EC 1.17.1.5 from Eubacterium barkeri (Clostridium barkeri) (see paper)
37% identity, 92% coverage: 3:141/151 of query aligns to 7:149/157 of Q0QLF3
- C42 (= C38) binding
- C47 (= C43) binding
- C50 (= C46) binding
- C62 (= C58) binding
- C101 (= C93) binding
- C104 (= C96) binding
- C136 (= C128) binding
- C138 (= C130) binding
4zohC Crystal structure of glyceraldehyde oxidoreductase (see paper)
36% identity, 92% coverage: 3:141/151 of query aligns to 13:154/161 of 4zohC
- binding fe2/s2 (inorganic) cluster: C47 (= C38), S50 (≠ G41), C52 (= C43), G53 (= G44), C55 (= C46), C67 (= C58), C106 (= C93), G107 (= G94), C109 (= C96), C141 (= C128), C143 (= C130)
- binding pterin cytosine dinucleotide: Q105 (= Q92), C143 (= C130)
1ffuD Carbon monoxide dehydrogenase from hydrogenophaga pseudoflava which lacks the mo-pyranopterin moiety of the molybdenum cofactor (see paper)
33% identity, 93% coverage: 1:141/151 of query aligns to 4:148/156 of 1ffuD
- binding fe2/s2 (inorganic) cluster: C41 (= C38), S44 (≠ G41), H45 (≠ L42), C46 (= C43), G47 (= G44), C49 (= C46), C61 (= C58), C100 (= C93), G101 (= G94), C103 (= C96), C135 (= C128), C137 (= C130)
1ffvA Carbon monoxide dehydrogenase from hydrogenophaga pseudoflava (see paper)
33% identity, 93% coverage: 1:141/151 of query aligns to 3:147/155 of 1ffvA
- binding fe2/s2 (inorganic) cluster: I38 (≠ Y36), C40 (= C38), S43 (≠ G41), C45 (= C43), G46 (= G44), C48 (= C46), C60 (= C58), C99 (= C93), G100 (= G94), C102 (= C96), C134 (= C128), C136 (= C130)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q98 (= Q92), C136 (= C130)
P19921 Carbon monoxide dehydrogenase small chain; CO dehydrogenase subunit S; CO-DH S; EC 1.2.5.3 from Afipia carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC 32145 / OM5) (Oligotropha carboxidovorans) (see 2 papers)
35% identity, 91% coverage: 4:141/151 of query aligns to 8:150/166 of P19921
- C42 (= C38) binding
- C47 (= C43) binding
- C50 (= C46) binding
- C62 (= C58) binding
- C102 (= C93) binding
- C105 (= C96) binding
- C137 (= C128) binding
- C139 (= C130) binding
1n5wA Crystal structure of the cu,mo-co dehydrogenase (codh); oxidized form (see paper)
35% identity, 91% coverage: 4:141/151 of query aligns to 6:148/161 of 1n5wA
- binding flavin-adenine dinucleotide: S43 (≠ G41), H44 (≠ L42)
- binding fe2/s2 (inorganic) cluster: I38 (≠ Y36), G39 (= G37), C40 (= C38), S43 (≠ G41), C45 (= C43), G46 (= G44), C48 (= C46), C60 (= C58), C100 (= C93), G101 (= G94), C103 (= C96), C135 (= C128), C137 (= C130)
1n5wD Crystal structure of the cu,mo-co dehydrogenase (codh); oxidized form (see paper)
35% identity, 91% coverage: 4:141/151 of query aligns to 6:148/158 of 1n5wD
- binding flavin-adenine dinucleotide: S43 (≠ G41), H44 (≠ L42)
- binding fe2/s2 (inorganic) cluster: C40 (= C38), S43 (≠ G41), C45 (= C43), G46 (= G44), C48 (= C46), C60 (= C58), C100 (= C93), G101 (= G94), C103 (= C96), C135 (= C128), C137 (= C130)
- binding pterin cytosine dinucleotide: Q99 (= Q92), C137 (= C130)
1dgjA Crystal structure of the aldehyde oxidoreductase from desulfovibrio desulfuricans atcc 27774 (see paper)
38% identity, 93% coverage: 3:142/151 of query aligns to 5:151/906 of 1dgjA
- binding fe2/s2 (inorganic) cluster: V38 (≠ Y36), G39 (= G37), C40 (= C38), G41 (= G39), G43 (= G41), Q44 (≠ L42), C45 (= C43), G46 (= G44), C48 (= C46), R58 (= R56), C60 (= C58), C100 (= C93), G101 (= G94), C103 (= C96), C137 (= C128), C139 (= C130)
- binding pterin cytosine dinucleotide: Q99 (= Q92), C139 (= C130)
Sites not aligning to the query:
- active site: 391, 427, 503, 507, 535, 869, 870
- binding molybdenum (iv)oxide: 424, 535, 698, 869
- binding pterin cytosine dinucleotide: 423, 424, 535, 652, 655, 656, 657, 658, 697, 698, 700, 702, 703, 799, 800, 803, 804, 807, 865, 866, 867, 868, 869
3zyvB Crystal structure of the mouse liver aldehyde oxidase 3 (maox3) (see paper)
41% identity, 91% coverage: 6:143/151 of query aligns to 8:161/1262 of 3zyvB
- binding fe2/s2 (inorganic) cluster: Y39 (= Y36), C41 (= C38), G42 (= G39), G44 (= G41), C46 (= C43), C49 (= C46), T69 (vs. gap), C71 (= C58), C111 (= C93), G112 (= G94), C114 (= C96), C146 (= C128), C148 (= C130)
Sites not aligning to the query:
- active site: 716, 751, 829, 833, 861, 1207, 1208
- binding flavin-adenine dinucleotide: 227, 229, 230, 231, 232, 233, 234, 267, 303, 304, 312, 313, 316, 317, 319, 325, 326, 366, 392, 393
G3X982 Aldehyde oxidase 3; Aldehyde oxidase homolog 1; Azaheterocycle hydroxylase 3; EC 1.2.3.1; EC 1.17.3.- from Mus musculus (Mouse) (see paper)
41% identity, 91% coverage: 6:143/151 of query aligns to 14:167/1335 of G3X982
- C47 (= C38) binding
- C52 (= C43) binding
- C55 (= C46) binding
- C77 (= C58) binding
- Q116 (= Q92) binding
- C117 (= C93) binding
- C120 (= C96) binding
- C152 (= C128) binding
- C154 (= C130) binding
Sites not aligning to the query:
- 264:271 binding
- 354 binding
- 358 binding
- 367 binding
- 411 binding
- 802 binding
- 807 A→V: No effect on kinetic constants with smaller substrates like benzaldehyde or phthalazine. Decreases substrate affinity and slightly increases catalytic efficiency for bulkier substrates like phenanthridine.
- 885 Y→M: Slightly decreases substrate affinity but no effect on activity with smaller substrates like benzaldehyde or phthalazine. Increases catalytic efficiency with bulkier substrates like phenanthridine or more charged substrates like N1-methylnicotinamide.
- 889 K→H: No effect on substrate affinity but decreases catalytic efficiency for smaller substrates like benzaldehyde or phthalazine. Increases substrate affinity and activity for bulkier substrates like phenanthridine.
- 1043 binding
- 1199 binding
- 1266 E→Q: Loss of activity with different N-heterocyclic compounds as substrates. 60% reduction of activity with benzaldehyde.
4usaA Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with trans-cinnamaldehyde (see paper)
37% identity, 92% coverage: 4:142/151 of query aligns to 6:151/907 of 4usaA
- binding fe2/s2 (inorganic) cluster: V38 (≠ Y36), C40 (= C38), E41 (≠ G39), G43 (= G41), C45 (= C43), G46 (= G44), C48 (= C46), R58 (= R56), C60 (= C58), C100 (= C93), G101 (= G94), C103 (= C96), C137 (= C128), C139 (= C130)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q92), C139 (= C130)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding bicarbonate ion: 460, 531, 532, 535, 539
- binding hydrocinnamic acid: 255, 425, 494, 497, 535, 626
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
4us9A Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with 3- phenylpropionaldehyde (see paper)
37% identity, 92% coverage: 4:142/151 of query aligns to 6:151/907 of 4us9A
- binding fe2/s2 (inorganic) cluster: V38 (≠ Y36), C40 (= C38), E41 (≠ G39), G43 (= G41), C45 (= C43), G46 (= G44), C48 (= C46), R58 (= R56), C60 (= C58), C100 (= C93), G101 (= G94), C103 (= C96), C137 (= C128), C139 (= C130)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q92), C139 (= C130)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding 3-phenylpropanal: 255, 257, 258, 752
- binding bicarbonate ion: 460, 498, 531, 532, 535, 539, 890, 892
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
4us8A Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with benzaldehyde (see paper)
37% identity, 92% coverage: 4:142/151 of query aligns to 6:151/907 of 4us8A
- binding fe2/s2 (inorganic) cluster: V38 (≠ Y36), C40 (= C38), E41 (≠ G39), G43 (= G41), C45 (= C43), G46 (= G44), C48 (= C46), R58 (= R56), C60 (= C58), C100 (= C93), G101 (= G94), C103 (= C96), C137 (= C128), C139 (= C130)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q92), C139 (= C130)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding bicarbonate ion: 460, 498, 531, 532, 535, 539
- binding benzaldehyde: 255, 255, 394, 425, 425, 425, 425, 497, 497, 501, 531, 535, 535, 626, 626, 626, 694, 696, 697
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
Query Sequence
>6939345 FitnessBrowser__SB2B:6939345
MTSLKINGRLFTLDADPKMPLLWALRDLMGLTGTKYGCGAGLCGACTVHVDGEPMRACLT
SIKSLEGKSVTTIEGLADEQLKDSWRRHKVPQCGFCQAGQLMSAAALVSKHPRPSDSQID
EAMAGNICRCGTYTRIRAAIKDYQGKQEAKS
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory