SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 6939439 Sama_3531 molybdenum ABC transporter, ATP-binding protein (RefSeq) to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
41% identity, 61% coverage: 22:248/370 of query aligns to 37:259/378 of P69874

query
sites
P69874

D

D

F

L

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T

C

I

R

N

A

N

G

G

E

E

F
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F

L

L

A

T

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L

G

G

P

P

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S

G

G

G
x
C

G

G

K

K

T

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L

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L
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L

R

R

M

L

I

I

A

A

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G

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T

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E

D

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G

S

-

I

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C

-

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K

-

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M

F
x
L

D

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N

D

E

E

D

G

I

I

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H

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P

P

A

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N

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M

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E

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L

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L

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A

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L

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D

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Y

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M

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I

F45 (= F30) mutation to L: Lower ATPase activity and transport efficiency.
C54 (≠ G39) mutation to T: Loss of ATPase activity and transport.
L60 (= L45) mutation to F: Lower ATPase activity and transport efficiency.
L76 (≠ F67) mutation to P: Lower ATPase activity and transport efficiency.
V135 (= V124) mutation to M: Loss of ATPase activity and transport.
D172 (= D161) mutation to N: Loss of ATPase activity and transport.

Sites not aligning to the query:

26 C→A: Lower ATPase activity and transport efficiency.
27 F→L: Lower ATPase activity and transport efficiency.
276 C→A: Lower ATPase activity and transport efficiency.
297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

1g291 Malk (see paper)
41% identity, 59% coverage: 20:238/370 of query aligns to 21:241/372 of 1g291

query
sites
1g291

E

E

A

M

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A

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P

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S

G
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G

G
x
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G
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G

K
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K

T
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T

L

T

L

L

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R

M

M

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I

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L

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x
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M

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L

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A

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G

G

binding magnesium ion: D69 (= D68), E71 (≠ D70), K72 (≠ E71), K79 (≠ Q78), D80 (≠ N79), Y228 (≠ F225), D229 (≠ S226)
binding pyrophosphate 2-: P37 (= P36), S38 (= S37), G39 (= G38), C40 (≠ G39), G41 (= G40), K42 (= K41), T43 (= T42), T44 (= T43)

Sites not aligning to the query:

binding magnesium ion: 292, 293, 341, 342, 359

2d62A Crystal structure of multiple sugar binding transport atp- binding protein
40% identity, 58% coverage: 24:238/370 of query aligns to 28:244/375 of 2d62A

query
sites
2d62A

S

E

C

I

R

K

A

D

G

G

E

E

F

F

L

L

A

V

V

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G

G

P
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P

S
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S

G
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G

G
x
C

G
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G

K
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K

T
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T

L

T

L

L

R

R

M

M

I

I

A

A

G

G

L

L

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K

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M

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L

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G

D

N

N

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M

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L

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I

G

G

binding pyrophosphate 2-: P40 (= P36), S41 (= S37), G42 (= G38), C43 (≠ G39), G44 (= G40), K45 (= K41), T46 (= T42), T47 (= T43)

3d31A Modbc from methanosarcina acetivorans (see paper)
31% identity, 93% coverage: 23:365/370 of query aligns to 21:347/348 of 3d31A

query
sites
3d31A

F

L

S

K

C

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E

A

S

G

G

E

E

F

Y

L

F

A

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I

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P

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T

G

G

G

A

G

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K

K

T

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L

L

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M

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A

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G

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C

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A

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M

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E

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A

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A

D

E

C

-

A

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K

Q

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P

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F

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S

K

I

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A

S

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G

Q

R

S

L
x
S

C
x
I

R
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R

S

-

F

-

N

N

I

S

L

L

P

Q

I

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H

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D

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A

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A

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L

N

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I
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I

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x
T

L

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x
S

A

A

H

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K

E

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M

A

E

L

L

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G

G

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A

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L

F

K
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K

S
x
A

D
x
S

Q

S

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V

H

H

I

V

L

L

binding tungstate(vi)ion: S286 (≠ L299), I287 (≠ C300), R288 (= R301), I319 (= I337), T320 (≠ P338), S323 (≠ L341), K340 (= K358), A341 (≠ S359), S342 (≠ D360)

1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
33% identity, 93% coverage: 22:366/370 of query aligns to 26:345/353 of 1vciA

query
sites
1vciA

D

N

F

L

S

T

C

I

R

K

A

D

G

G

E

E

F

F

L

L

A

V

V

L

G

G

P
|
P

S
|
S

G
|
G

G
x
C

G
|
G

K
|
K

T
|
T

L

T

L

L

R

R

M

M

I

I

A

A

G

G

L

L

A

E

K

E

P

P

E

T

N

E

G

G

S

-

I

-

R

-

C

-

G

-

K

-

R

R

I

I

W

Y

F

F

D

G

S

D

D

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E

D

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V

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C

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P

P

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K

N

D

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R

H

N

I

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F

M

V

V

P

F

Q

Q

H

H

F

M

G

T

L

V

F

Y

P

E

K

-

L

-

S

-

A

-

L

-

G

-

N

N

I

I

M

A

A

F

A

P

L

L

D

K

H

K

L

F

P

P

S

K

Q

D

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E

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I

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D

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K

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R

A

W

A

A

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E

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K

L

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N

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L

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G

E

L

L

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L

D

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R

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Y

P

P

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A

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Q

L

L

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S

G

G

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Q

K

R

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Q

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V

A

A

L

V

A

A

R

R

A

A

L

I

A

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R

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E

E

P

P

R

D

V

V

L

L

L

M

D

D

E

E

P

P

F

L

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S

A

N

V

L

D

D

R

A

E

K

T

L

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E

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R

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L

M

Y

R

L

A

E

E

L

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A

K

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K

L

L

K

Q

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Q

E

K

L

L

N

K

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P

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T

I

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M

Y

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T

T

H

H

D

D

L

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N

V

E

E

A

A

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M

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D

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M

L

L

L

Q

Q

H

I

G

G

T

S

P

P

Q

T

D

E

V

V

F

Y

S

L

R

R

P

P

R

N

N

S

E

V

A

F

V

V

A

A

R

T

Q

F

M

I

G

G

L

A

-

P

-

E

R

M

N

N

I

I

F

L

N

E

A

V

H

S

V

V

V

-

A

-

Q

-

D

-

C

-

A

-

K

-

G

G

V

D

T

G

W

Y

L

L

K

E

-

G

-

R

-

G

F

F

G

R

D

I

R

E

L

L

I

P

A

Q

S

M

D

D

S

L

L

L

P

-

R

K

V

D

N

Y

I

V

G

G

D

K

K

T

V

V

R

L

W

F

V

G

I

I

P

R

N

P

Q

E

G

H

I

M

R

T

F

V

N

E

S

G

I

V

A

H

S

M

G

K

R

R

L

T

C

A

R

R

S

L

F

I

N

G

I

-

L

-

P

-

I

-

H

K

I

V

D

D

N

F

M

V

L

E

T

A

L

L

G

G

E

T

S

D

V

T

R

I

L

L

E

H

A

V

S

K

V

F

Q

G

D

D

S

-

G

-

E

E

R

L

L

V

N

K

V

V

E

K

I

L

P

P

L

G

H

H

L

-

A

-

H

-

K

-

L

I

A

P

L

I

T

E

Q

P

G

G

A

R

A

E

T

V

E

K

V

V

A

I

L

M

K

D

S

L

D

D

Q

M

V

I

H

H

I

V

L

F

E

D

binding adenosine-5'-triphosphate: P40 (= P36), S41 (= S37), G42 (= G38), C43 (≠ G39), G44 (= G40), K45 (= K41), T46 (= T42), T47 (= T43)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 16, 19

7ahhC Opua inhibited inward-facing, sbd docked (see paper)
38% identity, 58% coverage: 20:233/370 of query aligns to 44:259/382 of 7ahhC

query
sites
7ahhC

E

D

A

T

D

N

F

F

S

E

C

I

R

N

A

E

G

G

E

E

F

I

L

F

A

V

V

I

V

M

G

G

P

L

S
|
S

G
|
G

G
x
S

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

M

L

I

L

A

N

G

R

L

L

A

I

K

E

P

P

E

T

N

S

G

G

S

-

I

-

R

-

C

-

G

-

K

-

R

K

I

I

W

F

F

I

D

D

S

N

D

Q

E

D

G

V

I

A

H

T

C

L

S

N

P

K

Q

E

N

D

-

L

-

Q

-

V

-

R

R

K

H

T

I

M

G

S

F

M

V

V

P

F

Q

Q

H

N

F

F

G

G

L

L

F

F

P

P

K

H

L

R

S

T

A

I

L

L

G

E

N

N

I

T

M

E

A

Y

A

G

L

L

D

E

-

V

-

Q

H

N

L

V

P

P

S

K

Q

E

E

E

R

R

P

K

R

R

A

A

L

E

A

K

A

A

L

L

E

D

K

N

V

A

N

N

L

L

H

L

G

D

L

F

T

K

D

D

R

Q

L

Y

P

P

S

K

Q

Q

L

L

S

S

G

G

Q

M

K

Q

Q

Q

R

R

V

V

A

G

L

L

A

A

R

R

A

A

L

L

A

A

R

N

E

D

P

P

R

E

V

I

L

L

L

M

D
|
D

E
|
E

P

A

F

F

S

S

A

A

V

L

D

D

R

P

E

L

T

I

R

R

E

R

R

E

L

M

Y

Q

L

D

E

E

L

L

A

L

R

E

L

L

K

Q

A

A

E

K

L

F

N

Q

I

K

P

T

V

I

I

I

M

F

V

V

T

S

H

H

D

D

L

L

N

N

E

E

A

A

L

L

L

R

L

I

A

G

D

D

S

R

M

I

L

A

L

I

I

M

S

K

Q

D

G

G

H

K

M

I

L

M

Q

Q

H

I

G

G

T

T

P

G

Q

E

D

E

V

I

F

L

S

T

R

N

P

P

R

A

N

N

E

D

A

Y

V

V

binding phosphoaminophosphonic acid-adenylate ester: S61 (= S37), G62 (= G38), S63 (≠ G39), G64 (= G40), K65 (= K41), S66 (≠ T42), T67 (= T43), D187 (= D161), E188 (= E162)

Sites not aligning to the query:

7aheC Opua inhibited inward facing (see paper)
38% identity, 58% coverage: 20:233/370 of query aligns to 44:259/382 of 7aheC

query
sites
7aheC

E

D

A

T

D

N

F

F

S

E

C

I

R

N

A

E

G

G

E

E

F

I

L

F

A

V

V

I

V

M

G

G

P

L

S

S

G

G

G

S

G

G

K

K

T

S

T

T

L

L

R

R

M

L

I

L

A

N

G

R

L

L

A

I

K

E

P

P

E

T

N

S

G

G

S

-

I

-

R

-

C

-

G

-

K

-

R

K

I

I

W

F

F

I

D

D

S

N

D

Q

E

D

G

V

I

A

H

T

C

L

S

N

P

K

Q

E

N

D

-

L

-

Q

-

V

-

R

R

K

H

T

I

M

G

S

F

M

V

V

P

F

Q

Q

H

N

F

F

G

G

L

L

F

F

P

P

K

H

L

R

S

T

A

I

L

L

G

E

N

N

I

T

M

E

A

Y

A

G

L

L

D

E

-

V

-

Q

H

N

L

V

P

P

S

K

Q

E

E

E

R

R

P

K

R

R

A

A

L

E

A

K

A

A

L

L

E

D

K

N

V

A

N

N

L

L

H

L

G

D

L

F

T

K

D

D

R

Q

L

Y

P

P

S

K

Q

Q

L

L

S

S

G

G

Q

M

K

Q

Q

Q

R

R

V

V

A

G

L

L

A

A

R

R

A

A

L

L

A

A

R

N

E

D

P

P

R

E

V

I

L

L

L

M

D

D

E

E

P

A

F

F

S

S

A

A

V

L

D

D

R

P

E

L

T

I

R

R

E

R

R

E

L

M

Y

Q

L

D

E

E

L

L

A

L

R

E

L

L

K

Q

A

A

E

K

L

F

N

Q

I

K

P

T

V

I

I

I

M

F

V

V

T

S

H

H

D

D

L

L

N

N

E

E

A

A

L

L

L

R

L

I

A

G

D

D

S

R

M

I

L

A

L

I

I

M

S

K

Q

D

G

G

H

K

M

I

L

M

Q

Q

H

I

G

G

T

T

P

G

Q

E

D

E

V

I

F

L

S

T

R

N

P

P

R

A

N

N

E

D

A

Y

V

V

Sites not aligning to the query:

binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 295, 296, 297, 298, 357, 372

7ahdC Opua (e190q) occluded (see paper)
37% identity, 58% coverage: 20:233/370 of query aligns to 44:259/260 of 7ahdC

query
sites
7ahdC

E

D

A

T

D

N

F

F

S

E

C

I

R

N

A

E

G

G

E

E

F

I

L

F

A

V

V

I

V

M

G

G

P

L

S
|
S

G
|
G

G
x
S

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

M

L

I

L

A

N

G

R

L

L

A

I

K

E

P

P

E

T

N

S

G

G

S

-

I

-

R

-

C

-

G

-

K

-

R

K

I

I

W

F

F

I

D

D

S

N

D

Q

E

D

G

V

I

A

H

T

C

L

S

N

P

K

Q

E

N

D

-

L

-

Q

-

V

-

R

R

K

H

T

I

M

G

S

F

M

V

V

P

F

Q
|
Q

H

N

F

F

G

G

L

L

F

F

P

P

K

H

L

R

S

T

A

I

L

L

G

E

N

N

I

T

M

E

A

Y

A

G

L

L

D

E

-

V

-

Q

H

N

L

V

P

P

S

K

Q

E

E

E

R

R

P

K

R

R

A

A

L

E

A

K

A

A

L

L

E

D

K

N

V

A

N

N

L

L

H

L

G

D

L

F

T

K

D

D

R

Q

L

Y

P

P

S
x
K

Q
|
Q

L

L

S
|
S

G

G

G
|
G

Q
x
M

K

Q

Q

Q

R

R

V

V

A

G

L

L

A

A

R

R

A

A

L

L

A

A

R

N

E

D

P

P

R

E

V

I

L

L

L

M

D

D

E
x
Q

P

A

F

F

S

S

A

A

V

L

D

D

R

P

E

L

T

I

R

R

E

R

R

E

L

M

Y

Q

L

D

E

E

L

L

A

L

R

E

L

L

K

Q

A

A

E

K

L

F

N

Q

I

K

P

T

V

I

I

I

M

F

V

V

T

S

H
|
H

D

D

L

L

N

N

E

E

A

A

L

L

L

R

L

I

A

G

D

D

S

R

M

I

L

A

L

I

I

M

S

K

Q

D

G

G

H

K

M

I

L

M

Q

Q

H

I

G

G

T

T

P

G

Q

E

D

E

V

I

F

L

S

T

R

N

P

P

R

A

N

N

E

D

A

Y

V

V

binding adenosine-5'-triphosphate: S61 (= S37), G62 (= G38), S63 (≠ G39), G64 (= G40), K65 (= K41), S66 (≠ T42), T67 (= T43), Q111 (= Q87), K161 (≠ S135), Q162 (= Q136), S164 (= S138), G166 (= G140), M167 (≠ Q141), Q188 (≠ E162), H221 (= H195)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12, 29, 39

1oxvD Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
34% identity, 64% coverage: 21:255/370 of query aligns to 24:259/353 of 1oxvD

query
sites
1oxvD

A

V

D

N

F

I

S

N

C

I

R

E

A

N

G

G

E

E

F

R

L

F

A

G

V

I

V

L

G

G

P
|
P

S
|
S

G
|
G

G
x
A

G
|
G

K
|
K

T
|
T

L

F

L

M

R

R

M

I

I

I

A

A

G

G

L

L

A

D

K

V

P

P

E

S

N

T

G

G

S

E

I

L

R

Y

C

F

G

D

K

D

R

R

I

L

W

-

F

V

D

A

S

S

D

N

E

G

G

K

I

L

H

I

C

V

S

P

P

P

Q

E

N

D

R

R

H

K

I

I

G

G

F

M

V

V

P

F

Q
|
Q

H

T

F

W

G

A

L

L

F

Y

P

P

K

N

L

L

S

T

A

A

L

F

G

E

N

N

I

I

M

A

A

F

A

P

L

L

D

T

H

N

L

M

-

K

-

M

P

S

S

K

Q

E

E

E

R

I

R

R

P

K

R

R

A

V

L

E

A

E

A

V

L

A

E

K

K

I

V

L

N

D

L

I

H

H

G

H

L

V

T

L

D

N

R

H

L

F

P

P

S

R

Q

E

L

L

S

S

G

G

Q

Q

K

Q

Q

Q

R

R

V

V

A

A

L

L

A

A

R

R

A

A

L

L

A

V

R

K

E

D

P

P

R

S

V

L

L

L

D

D

E
|
E

P

P

F

F

S

S

A

N

V

L

D

D

R

A

E

R

T

M

R

R

E

D

R

S

L

A

Y

R

L

A

E

L

L

V

A

K

R

E

L

V

K

Q

A

S

E

R

L

L

N

G

I

V

P

T

V

L

I

L

M

V

V

V

T

S

H

H

D

D

L

P

N

A

E

D

A

I

L

F

L

A

L

I

A

A

D

D

S

R

M

V

L

G

L

V

I

L

S

V

Q

K

G

G

H

K

M

L

L

V

Q

Q

H

V

G

G

T

K

P

P

Q

E

D

D

V

L

F

Y

S

D

R

N

P

P

R

V

N

S

E

I

A

Q

V

V

A

A

R

S

Q

L

M

I

G

G

L

E

R

I

N

N

I

E

F

L

N

E

A

G

H

K

V

V

V

T

A

N

Q

E

D

G

C

V

A

V

K

I

G

G

binding phosphoaminophosphonic acid-adenylate ester: P39 (= P36), S40 (= S37), G41 (= G38), A42 (≠ G39), G43 (= G40), K44 (= K41), T45 (= T42), T46 (= T43), Q89 (= Q87), E166 (= E162)
binding magnesium ion: T45 (= T42), Q89 (= Q87)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 13, 18, 20

1oxvA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
34% identity, 64% coverage: 21:255/370 of query aligns to 24:259/353 of 1oxvA

query
sites
1oxvA

A

V

D

N

F

I

S

N

C

I

R

E

A

N

G

G

E

E

F

R

L

F

A

G

V

I

V

L

G

G

P
|
P

S
|
S

G
|
G

G
x
A

G
|
G

K
|
K

T
|
T

L

F

L

M

R

R

M

I

I

I

A

A

G

G

L

L

A

D

K

V

P

P

E

S

N

T

G

G

S

E

I

L

R

Y

C

F

G

D

K

D

R

R

I

L

W

-

F

V

D

A

S

S

D

N

E

G

G

K

I

L

H

I

C

V

S

P

P

P

Q

E

N

D

R

R

H

K

I

I

G

G

F

M

V

V

P

F

Q

Q

H

T

F

W

G

A

L

L

F

Y

P

P

K

N

L

L

S

T

A

A

L

F

G

E

N

N

I

I

M

A

A

F

A

P

L

L

D

T

H

N

L

M

-

K

-

M

P

S

S

K

Q

E

E

E

R

I

R

R

P

K

R

R

A

V

L

E

A

E

A

V

L

A

E

K

K

I

V

L

N

D

L

I

H

H

G

H

L

V

T

L

D

N

R

H

L

F

P

P

S

R

Q

E

L

L

S

S

G

G

Q

Q

K

Q

Q

Q

R

R

V

V

A

A

L

L

A

A

R

R

A

A

L

L

A

V

R

K

E

D

P

P

R

S

V

L

L

L

D

D

E
|
E

P

P

F

F

S

S

A

N

V

L

D

D

R

A

E

R

T

M

R

R

E

D

R

S

L

A

Y

R

L

A

E

L

L

V

A

K

R

E

L

V

K

Q

A

S

E

R

L

L

N

G

I

V

P

T

V

L

I

L

M

V

V

V

T

S

H

H

D

D

L

P

N

A

E

D

A

I

L

F

L

A

L

I

A

A

D

D

S

R

M

V

L

G

L

V

I

L

S

V

Q

K

G

G

H

K

M

L

L

V

Q

Q

H

V

G

G

T

K

P

P

Q

E

D

D

V

L

F

Y

S

D

R

N

P

P

R

V

N

S

E

I

A

Q

V

V

A

A

R

S

Q

L

M

I

G

G

L

E

R

I

N

N

I

E

F

L

N

E

A

G

H

K

V

V

V

T

A

N

Q

E

D

G

C

V

A

V

K

I

G

G

binding phosphoaminophosphonic acid-adenylate ester: P39 (= P36), S40 (= S37), G41 (= G38), A42 (≠ G39), G43 (= G40), K44 (= K41), T45 (= T42), T46 (= T43), E166 (= E162)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 13, 18, 20

1oxuA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
34% identity, 64% coverage: 21:255/370 of query aligns to 24:259/353 of 1oxuA

query
sites
1oxuA

A

V

D

N

F

I

S

N

C

I

R

E

A

N

G

G

E

E

F

R

L

F

A

G

V

I

V

L

G

G

P

P

S
|
S

G
|
G

G
x
A

G
|
G

K
|
K

T
|
T

L

F

L

M

R

R

M

I

I

I

A

A

G

G

L

L

A

D

K

V

P

P

E

S

N

T

G

G

S

E

I

L

R

Y

C

F

G

D

K

D

R

R

I

L

W

-

F

V

D

A

S

S

D

N

E

G

G

K

I

L

H

I

C

V

S

P

P

P

Q

E

N

D

R

R

H

K

I

I

G

G

F

M

V

V

P

F

Q

Q

H

T

F

W

G

A

L

L

F

Y

P

P

K

N

L

L

S

T

A

A

L

F

G

E

N

N

I

I

M

A

A

F

A

P

L

L

D

T

H

N

L

M

-

K

-

M

P

S

S

K

Q

E

E

E

R

I

R

R

P

K

R

R

A

V

L

E

A

E

A

V

L

A

E

K

K

I

V

L

N

D

L

I

H

H

G

H

L

V

T

L

D

N

R

H

L

F

P

P

S

R

Q

E

L

L

S

S

G

G

Q

Q

K

Q

Q

Q

R

R

V

V

A

A

L

L

A

A

R

R

A

A

L

L

A

V

R

K

E

D

P

P

R

S

V

L

L

L

D

D

E

E

P

P

F

F

S

S

A

N

V

L

D

D

R

A

E

R

T

M

R

R

E

D

R

S

L

A

Y

R

L

A

E

L

L

V

A

K

R

E

L

V

K

Q

A

S

E

R

L

L

N

G

I

V

P

T

V

L

I

L

M

V

V

V

T

S

H

H

D

D

L

P

N

A

E

D

A

I

L

F

L

A

L

I

A

A

D

D

S

R

M

V

L

G

L

V

I

L

S

V

Q

K

G

G

H

K

M

L

L

V

Q

Q

H

V

G

G

T

K

P

P

Q

E

D

D

V

L

F

Y

S

D

R

N

P

P

R

V

N

S

E

I

A

Q

V

V

A

A

R

S

Q

L

M

I

G

G

L

E

R

I

N

N

I

E

F

L

N

E

A

G

H

K

V

V

V

T

A

N

Q

E

D

G

C

V

A

V

K

I

G

G

binding adenosine-5'-diphosphate: S40 (= S37), G41 (= G38), A42 (≠ G39), G43 (= G40), K44 (= K41), T45 (= T42), T46 (= T43)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 13, 20

Q97UY8 Glucose import ATP-binding protein GlcV; EC 7.5.2.- from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
34% identity, 64% coverage: 21:255/370 of query aligns to 24:259/353 of Q97UY8

query
sites
Q97UY8

A

V

D

N

F

I

S

N

C

I

R

E

A

N

G

G

E

E

F

R

L

F

A

G

V

I

V

L

G

G

P

P

S

S

G

G

G

A

G

G

K

K

T

T

L

F

L

M

R

R

M

I

I

I

A

A

G

G

L

L

A

D

K

V

P

P

E

S

N

T

G

G

S

E

I

L

R

Y

C

F

G

D

K

D

R

R

I

L

W

-

F

V

D

A

S

S

D

N

E

G

G

K

I

L

H

I

C

V

S

P

P

P

Q

E

N

D

R

R

H

K

I

I

G

G

F

M

V

V

P

F

Q

Q

H

T

F

W

G

A

L

L

F

Y

P

P

K

N

L

L

S

T

A

A

L

F

G

E

N

N

I

I

M

A

A

F

A

P

L

L

D

T

H

N

L

M

-

K

-

M

P

S

S

K

Q

E

E

E

R

I

R

R

P

K

R

R

A

V

L

E

A

E

A

V

L

A

E

K

K

I

V

L

N

D

L

I

H

H

G

H

L

V

T

L

D

N

R

H

L

F

P

P

S

R

Q

E

L

L

S
|
S

G

G

G
|
G

Q

Q

K

Q

Q

Q

R

R

V

V

A

A

L

L

A

A

R

R

A

A

L

L

A

V

R

K

E

D

P

P

R

S

V

L

L

L

D

D

E
|
E

P

P

F

F

S

S

A

N

V

L

D

D

R

A

E

R

T

M

R

R

E

D

R

S

L

A

Y

R

L

A

E

L

L

V

A

K

R

E

L

V

K

Q

A

S

E

R

L

L

N

G

I

V

P

T

V

L

I

L

M

V

V

V

T

S

H

H

D

D

L

P

N

A

E

D

A

I

L

F

L

A

L

I

A

A

D

D

S

R

M

V

L

G

L

V

I

L

S

V

Q

K

G

G

H

K

M

L

L

V

Q

Q

H

V

G

G

T

K

P

P

Q

E

D

D

V

L

F

Y

S

D

R

N

P

P

R

V

N

S

E

I

A

Q

V

V

A

A

R

S

Q

L

M

I

G

G

L

E

R

I

N

N

I

E

F

L

N

E

A

G

H

K

V

V

V

T

A

N

Q

E

D

G

C

V

A

V

K

I

G

G

S142 (= S138) mutation to A: Decrease in ATPase activity. Can form dimers.
G144 (= G140) mutation to A: Loss of ATPase activity. Cannot form dimers. Forms an active heterodimer; when associated with A-166.
E166 (= E162) mutation to A: Loss of ATPase activity. Can form dimers in the presence of ATP-Mg(2+). Forms an active heterodimer; when associated with A-144.; mutation to Q: Strong decrease in ATPase activity. Can form dimers in the presence of ATP alone, without Mg(2+).

7cagC Mycobacterium smegmatis lpqy-sugabc complex in the catalytic intermediate state (see paper)
37% identity, 59% coverage: 20:238/370 of query aligns to 18:232/362 of 7cagC

query
sites
7cagC

E

E

A

F

D

S

F

M

S

T

C

I

R

A

A

D

G

G

E

E

F

F

L

I

A

I

V

L

V

V

G

G

P
|
P

S
|
S

G
|
G

G
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

T

L

L

R

N

M

M

I

I

A

A

G

G

L

L

A

E

K

E

P

I

E

T

N

S

G

G

S

E

I

L

R

R

C

I

G

G

-

G

K

E

R

R

I

V

W

N

F

-

D

-

S

-

D

-

E

-

G

-

I

-

H

E

C

K

S

A

P

P

Q

K

N

D

R

R

H

D

I

I

G

A

F

M

V

V

P

F

Q
|
Q

H

S

F

Y

G

A

L

L

F

Y

P

P

K

H

L

M

S

T

A

V

L

R

G

Q

N

N

I

I

M

A

-

F

-

P

A

L

A

T

L

L

D

A

H

K

L

V

P

P

S

K

Q

A

E

E

R

I

R

A

P

A

R

K

A

V

L

E

A

E

A

T

L

A

E

K

K

I

V

L

N

D

L

L

H

S

G

E

L
|
L

T

L

D

D

R
|
R

L

K

P

P

S

G

Q
|
Q

L

L

S
|
S

G
|
G

Q
|
Q

K

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

A

A

L

I

A

V

R

R

E

S

P

P

R

K

V

A

L

F

L

L

L

M

D

D

E

Q

P

P

F

L

S

S

A

N

V

L

D

D

R

A

E

K

T

L

R

R

E

V

R

Q

L

M

Y

R

L

A

E

E

L

I

A

S

R

R

L

L

K

Q

A

D

E

R

L

L

N

G

I

T

P

T

V

T

I

V

M

Y

V

V

T

T

H

H

D

D

L

Q

N

T

E

E

A

A

L

M

L

T

L

L

A

G

D

D

S

R

M

V

L

V

I

M

S

L

Q

A

G

G

H

E

M

V

L

Q

Q

Q

H

I

G

G

T

T

P

P

Q

D

D

E

V

L

F

Y

S

S

R

S

P

P

R

A

N

N

E

L

A

F

V

V

A

A

R

G

Q

F

M

I

G

G

binding adenosine-5'-triphosphate: P34 (= P36), S35 (= S37), G36 (= G38), C37 (≠ G39), G38 (= G40), K39 (= K41), S40 (≠ T42), T41 (= T43), Q79 (= Q87), L123 (= L129), R126 (= R132), Q130 (= Q136), S132 (= S138), G133 (= G139), G134 (= G140), Q135 (= Q141)
binding magnesium ion: S40 (≠ T42), Q79 (= Q87)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 14

P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
37% identity, 67% coverage: 22:269/370 of query aligns to 24:264/393 of P9WQI3

query
sites
P9WQI3

D

N

F

L

S

T

C

I

R

A

A

D

G

G

E

E

F

F

L

L

A

I

V

L

V

V

G

G

P

P

S

S

G

G

G

C

G

G

K

K

T

T

L

T

L

L

R

N

M

M

I

I

A

A

G

G

L

L

A

E

K

D

P

I

E

S

N

S

G

G

S

E

I

L

R

R

-

I

C

A

G

G

K

E

R

R

I

V

W

N

F

-

D

-

S

-

D

-

E

-

G

-

I

-

H

E

C

K

S

A

P

P

Q

K

N

D

R

R

H

D

I

I

G

A

F

M

V

V

P

F

Q

Q

H

S

F

Y

G

A

L

L

F

Y

P

P

K

H

L

M

S

T

A

V

L

R

G

Q

N

N

I

I

M

A

A

-

L

-

D

-

H

-

L

F

P

P

S

L

Q

T

E

L

R

A

R

K

P

M

R

R

A

K

L

A

A

D

A

I

L

A

E

Q

K

K

V

V

-

S

-

E

-

T

-

A

-

K

-

I

-

L

N

D

L

L

H

T

G

N

L

L

T

L

D

D

R

R

L

K

P

P

S

S

Q

Q

L

L

S

S

G

G

Q

Q

K

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

A

A

L

I

A

V

R

R

E

H

P

P

R

K

V

A

L

F

L

L

L

M

D

D

E

E

P

P

F

L

S

S

A

N

V

L

D

D

R

A

E

K

T

L

R

R

E

V

R

Q

L

M

Y

R

L

G

E

E

L

I

A

A

R

Q

L

L

K

Q

A

R

E

R

L

L

N

G

I

T

P

T

V

T

I

V

M

Y

V

V

T

T

H
|
H

D

D

L

Q

N

T

E

E

A

A

L

M

L

T

L

L

A

G

D

D

S

R

M

V

L

V

I

M

S

Y

Q

G

G

G

H

I

M

A

L

Q

Q

Q

H

I

G

G

T

T

P

P

Q

E

D

E

V

L

F

Y

S

E

R

R

P

P

R

A

N

N

E

L

A

F

V

V

A

A

R

G

Q

F

M

I

G

G

-

S

-

P

L

A

R

M

N

N

I

F

F

F

N

P

A

A

H

R

V

L

V

-

A

-

Q

-

D

-

C

T

A

A

K

I

G

G

V

L

T

T

W

-

L

L

K

P

F

F

G

G

D

E

R

V

L

T

I

L

A

A

S

P

D

E

H193 (= H195) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.

2awoA Crystal structure of the adp-mg-bound e. Coli malk (crystallized with adp-mg) (see paper)
38% identity, 59% coverage: 20:238/370 of query aligns to 20:234/372 of 2awoA

query
sites
2awoA

E

D

A

I

D

N

F

L

S

D

C

I

R

H

A

E

G

G

E

E

F

F

L

V

A

V

V

F

V

V

G

G

P

P

S
|
S

G
|
G

G
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

M

M

I

I

A

A

G

G

L

L

A

E

K

T

P

I

E

T

N

S

G

G

S

D

I

L

R

F

C

I

G

G

K

E

R

K

I

R

W

M

F

N

D

D

S

T

D

-

E

-

G

-

I

-

H

-

C

-

S

P

P

P

Q

A

N

E

R

R

H

G

I

V

G

G

F

M

V

V

P

F

Q

Q

H

S

F

Y

G

A

L

L

F

Y

P

P

K

H

L

L

S

S

A

V

L

A

G

E

N

N

I

M

M

S

A

F

A

G

L

L

D

K

H

L

L

A

P

G

S

A

Q

K

E

K

R

E

-

V

-

I

R

N

P

Q

R

R

A

V

L

N

A

Q

A

V

L

A

E

E

K

V

V

L

N

Q

L

L

H

A

G

H

L

L

T

L

D

D

R

R

L

K

P

P

S

K

Q

A

L

L

S

S

G

G

Q

Q

K

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

R

A

E

E

P

P

R

S

V

V

L

F

L

L

D
|
D

E

E

P

P

F

L

S

S

A

N

V

L

D

D

R

A

E

A

T

L

R

R

E

V

R

Q

L

M

Y

R

L

I

E

E

L

I

A

S

R

R

L

L

K

H

A

K

E

R

L

L

N

G

I

R

P

T

V

M

I

I

M

Y

V

V

T

T

H

H

D

D

L

Q

N

V

E

E

A

A

L

M

L

T

L

L

A

A

D

D

S

K

M

I

L

V

I

L

S

D

Q

A

G

G

H

R

M

V

L

A

Q

Q

H

V

G

G

T

K

P

P

Q

L

D

E

V

L

F

Y

S

H

R

Y

P

P

R

A

N

D

E

R

A

F

V

V

A

A

R

G

Q

F

M

I

G

G

binding adenosine-5'-diphosphate: S37 (= S37), G38 (= G38), C39 (≠ G39), G40 (= G40), K41 (= K41), S42 (≠ T42), T43 (= T43)
binding magnesium ion: S42 (≠ T42), D157 (= D161)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 17

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
38% identity, 59% coverage: 20:238/370 of query aligns to 21:235/371 of P68187

query
sites
P68187

E

D

A

I

D

N

F

L

S

D

C

I

R

H

A

E

G

G

E

E

F

F

L

V

A

V

V

F

V

V

G

G

P

P

S

S

G

G

G

C

G

G

K

K

T

S

T

T

L

L

R

R

M

M

I

I

A

A

G

G

L

L

A

E

K

T

P

I

E

T

N

S

G

G

S

D

I

L

R

F

C

I

G

G

K

E

R

K

I

R

W

M

F

N

D

D

S

T

D

-

E

-

G

-

I

-

H

-

C

-

S

P

P

P

Q

A

N

E

R

R

H

G

I

V

G

G

F

M

V

V

P

F

Q

Q

H

S

F

Y

G
x
A

L

L

F

Y

P

P

K

H

L

L

S

S

A

V

L

A

G

E

N

N

I

M

M

S

A

F

A

G

L

L

D

K

H

L

L

A

P

G

S

A

Q
x
K

E

K

R

E

-

V

-

I

R

N

P

Q

R

R

A
x
V

L

N

A

Q

A
x
V

L

A

E
|
E

K

V

V

L

N

Q

L

L

H
x
A

G

H

L

L

T

L

D

D

R

R

L

K

P

P

S

K

Q

A

L

L

S

S

G

G

G
|
G

Q

Q

K

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

R

A

E

E

P

P

R

S

V

V

L

F

L

L

D
|
D

E

E

P

P

F

L

S

S

A

N

V

L

D

D

R

A

E

A

T

L

R

R

E

V

R

Q

L

M

Y

R

L

I

E

E

L

I

A

S

R

R

L

L

K

H

A

K

E

R

L

L

N

G

I

R

P

T

V

M

I

I

M

Y

V

V

T

T

H

H

D

D

L

Q

N

V

E

E

A

A

L

M

L

T

L

L

A

A

D

D

S

K

M

I

L

V

I

L

S

D

Q

A

G

G

H

R

M

V

L

A

Q

Q

H

V

G

G

T

K

P

P

Q

L

D

E

V

L

F

Y

S

H

R

Y

P

P

R

A

N

D

E
x
R

A

F

V

V

A

A

R

G

Q

F

M

I

G

G

A85 (≠ G90) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ Q111) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (≠ A117) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ A120) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (= E122) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ H127) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G140) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D161) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
R228 (≠ E231) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.

Sites not aligning to the query:

241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
38% identity, 59% coverage: 20:238/370 of query aligns to 18:232/367 of 1q12A

query
sites
1q12A

E

D

A

I

D

N

F

L

S

D

C

I

R

H

A

E

G

G

E

E

F

F

L

V

A

V

V

F

V

V

G

G

P

P

S
|
S

G
|
G

G
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

M

M

I

I

A

A

G

G

L

L

A

E

K

T

P

I

E

T

N

S

G

G

S

D

I

L

R

F

C

I

G

G

K

E

R

K

I

R

W

M

F

N

D

D

S

T

D

-

E

-

G

-

I

-

H

-

C

-

S

P

P

P

Q

A

N

E

R

R

H

G

I

V

G

G

F

M

V

V

P

F

Q

Q

H

S

F

Y

G

A

L

L

F

Y

P

P

K

H

L

L

S

S

A

V

L

A

G

E

N

N

I

M

M

S

A

F

A

G

L

L

D

K

H

L

L

A

P

G

S

A

Q

K

E

K

R

E

-

V

-

I

R

N

P

Q

R

R

A

V

L

N

A

Q

A

V

L

A

E

E

K

V

V

L

N

Q

L

L

H

A

G

H

L

L

T

L

D

D

R
|
R

L

K

P

P

S
x
K

Q
x
A

L
|
L

S
|
S

G
|
G

Q
|
Q

K

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

R

A

E

E

P

P

R

S

V

V

L

F

L

L

D

D

E

E

P

P

F

L

S

S

A
x
N

V

L

D

D

R

A

E

A

T

L

R

R

E

V

R

Q

L

M

Y

R

L

I

E

E

L

I

A

S

R

R

L

L

K

H

A

K

E

R

L

L

N

G

I

R

P

T

V

M

I

I

M

Y

V

V

T

T

H
|
H

D

D

L

Q

N

V

E

E

A

A

L

M

L

T

L

L

A

A

D

D

S

K

M

I

L

V

I

L

S

D

Q

A

G

G

H

R

M

V

L

A

Q

Q

H

V

G

G

T

K

P

P

Q

L

D

E

V

L

F

Y

S

H

R

Y

P

P

R

A

N

D

E

R

A

F

V

V

A

A

R

G

Q

F

M

I

G

G

binding adenosine-5'-triphosphate: S35 (= S37), G36 (= G38), C37 (≠ G39), G38 (= G40), K39 (= K41), S40 (≠ T42), T41 (= T43), R126 (= R132), K129 (≠ S135), A130 (≠ Q136), L131 (= L137), S132 (= S138), G133 (= G139), G134 (= G140), Q135 (= Q141), N160 (≠ A166), H189 (= H195)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 10, 15

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
38% identity, 59% coverage: 20:238/370 of query aligns to 20:234/374 of 2awnB

query
sites
2awnB

E

D

A

I

D

N

F

L

S

D

C

I

R

H

A

E

G

G

E

E

F

F

L

V

A

V

V

F

V

V

G

G

P

P

S
|
S

G
|
G

G
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

M

M

I

I

A

A

G

G

L

L

A

E

K

T

P

I

E

T

N

S

G

G

S

D

I

L

R

F

C

I

G

G

K

E

R

K

I

R

W

M

F

N

D

D

S

T

D

-

E

-

G

-

I

-

H

-

C

-

S

P

P

P

Q

A

N

E

R

R

H

G

I

V

G

G

F

M

V

V

P

F

Q

Q

H

S

F

Y

G

A

L

L

F

Y

P

P

K

H

L

L

S

S

A

V

L

A

G

E

N

N

I

M

M

S

A

F

A

G

L

L

D

K

H

L

L

A

P

G

S

A

Q

K

E

K

R

E

-

V

-

I

R

N

P

Q

R

R

A

V

L

N

A

Q

A

V

L

A

E

E

K

V

V

L

N

Q

L

L

H

A

G

H

L

L

T

L

D

D

R

R

L

K

P

P

S

K

Q

A

L

L

S

S

G

G

Q

Q

K

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

R

A

E

E

P

P

R

S

V

V

L

F

L

L

D

D

E

E

P

P

F

L

S

S

A

N

V

L

D

D

R

A

E

A

T

L

R

R

E

V

R

Q

L

M

Y

R

L

I

E

E

L

I

A

S

R

R

L

L

K

H

A

K

E

R

L

L

N

G

I

R

P

T

V

M

I

I

M

Y

V

V

T

T

H

H

D

D

L

Q

N

V

E

E

A

A

L

M

L

T

L

L

A

A

D

D

S

K

M

I

L

V

I

L

S

D

Q

A

G

G

H

R

M

V

L

A

Q

Q

H

V

G

G

T

K

P

P

Q

L

D

E

V

L

F

Y

S

H

R

Y

P

P

R

A

N

D

E

R

A

F

V

V

A

A

R

G

Q

F

M

I

G

G

binding adenosine-5'-diphosphate: S37 (= S37), G38 (= G38), C39 (≠ G39), G40 (= G40), K41 (= K41), S42 (≠ T42), T43 (= T43)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 17

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
38% identity, 59% coverage: 20:238/370 of query aligns to 20:234/371 of 3puyA

query
sites
3puyA

E

D

A

I

D

N

F

L

S

D

C

I

R

H

A

E

G

G

E

E

F

F

L

V

A

V

V

F

V

V

G

G

P
|
P

S
|
S

G
|
G

G
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

M

M

I

I

A

A

G

G

L

L

A

E

K

T

P

I

E

T

N

S

G

G

S

D

I

L

R

F

C

I

G

G

K

E

R

K

I

R

W

M

F

N

D

D

S

T

D

-

E

-

G

-

I

-

H

-

C

-

S

P

P

P

Q

A

N

E

R

R

H

G

I

V

G

G

F

M

V

V

P

F

Q
|