SitesBLAST
Comparing 7022769 FitnessBrowser__ANA3:7022769 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6tnmA E. Coli aerobic trifunctional enzyme subunit-alpha (see paper)
62% identity, 100% coverage: 1:714/716 of query aligns to 1:714/719 of 6tnmA
- active site: A68 (= A68), F73 (= F73), G116 (= G116), E119 (= E119), P138 (= P138), E139 (= E139), G147 (= G147), N271 (≠ Q271), S429 (= S429), H450 (= H450), E462 (= E462), N500 (= N500)
- binding adenosine-5'-triphosphate: D343 (= D343), I344 (= I344), V400 (= V400), V401 (= V401), V406 (= V406), K584 (= K584)
P21177 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Escherichia coli (strain K12) (see 2 papers)
62% identity, 100% coverage: 1:714/716 of query aligns to 1:714/729 of P21177
- G116 (= G116) mutation to F: Absence of both enoyl-CoA hydratase and 3-hydroxyacyl-CoA epimerase activities. Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase is only slightly affected.
- G322 (= G322) mutation to A: 10-fold increase in KM for NADH.
- H450 (= H450) active site, For 3-hydroxyacyl-CoA dehydrogenase activity; mutation H->A,Q: Almost complete loss of 3-hydroxyacyl-CoA dehydrogenase activity.
1wdlA Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form ii (native4) (see paper)
61% identity, 100% coverage: 1:714/716 of query aligns to 1:714/715 of 1wdlA
- active site: A69 (= A68), N89 (≠ I88), N93 (= N92), G117 (= G116), E120 (= E119), P139 (= P138), E140 (= E139), P147 (= P146), G148 (= G147), S430 (= S429), H451 (= H450), E463 (= E462), N501 (= N500)
- binding nicotinamide-adenine-dinucleotide: A322 (= A321), I324 (= I323), M325 (= M324), D344 (= D343), I345 (= I344), A400 (= A399), V401 (= V400), E403 (= E402), N428 (= N427), T429 (= T428), S430 (= S429)
P28793 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Pseudomonas fragi (see paper)
61% identity, 100% coverage: 1:714/716 of query aligns to 1:714/715 of P28793
1wdmA Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form i (native3) (see paper)
61% identity, 100% coverage: 1:714/716 of query aligns to 1:706/707 of 1wdmA
- active site: A69 (= A68), N89 (≠ I88), N93 (= N92), G117 (= G116), E120 (= E119), P139 (= P138), E140 (= E139), P147 (= P146), G148 (= G147), S430 (= S429), H451 (= H450), E463 (= E462), N501 (= N500)
- binding acetyl coenzyme *a: K142 (= K141), D297 (= D296), M459 (= M458), N501 (= N500), P534 (= P534), Y652 (= Y660), L658 (≠ P666)
- binding nicotinamide-adenine-dinucleotide: G321 (= G320), A322 (= A321), I324 (= I323), M325 (= M324), D344 (= D343), V401 (= V400), E403 (= E402), N428 (= N427), S430 (= S429), N454 (= N453)
6yswA E. Coli anaerobic trifunctional enzyme subunit-alpha in complex with coenzyme a
36% identity, 99% coverage: 7:715/716 of query aligns to 5:704/707 of 6yswA
- active site: A66 (= A68), I71 (≠ F73), A84 (≠ S86), Q88 (≠ N92), G112 (= G116), E115 (= E119), P136 (= P138), E137 (= E139), G145 (= G147), D264 (≠ Q271), S422 (= S429), H443 (= H450), E455 (= E462), N493 (= N500)
- binding coenzyme a: E23 (≠ S27), M25 (≠ V28), A66 (= A68), D67 (= D69), I68 (= I70), P136 (= P138), E137 (= E139), L140 (= L142), T290 (≠ Q297), K293 (= K300)
P40939 Trifunctional enzyme subunit alpha, mitochondrial; 78 kDa gastrin-binding protein; Monolysocardiolipin acyltransferase; TP-alpha; EC 2.3.1.-; EC 4.2.1.17; EC 1.1.1.211 from Homo sapiens (Human) (see 5 papers)
35% identity, 98% coverage: 16:715/716 of query aligns to 48:763/763 of P40939
- V282 (≠ M231) to D: in MTPD1; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852773
- I305 (≠ V255) to N: in MTPD1; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852774
- L342 (≠ I292) to P: in LCHAD deficiency; dbSNP:rs137852772
- E510 (= E462) active site, For hydroxyacyl-coenzyme A dehydrogenase activity; to Q: in AFLP and LCHAD deficiency; loss of long-chain-3-hydroxyacyl-CoA dehydrogenase activity; dbSNP:rs137852769
4b3iA Crystal structure of mycobacterium tuberculosis fatty acid beta-oxidation complex with coenzymea bound at the hydratase active sites (see paper)
35% identity, 96% coverage: 7:691/716 of query aligns to 16:716/731 of 4b3iA
- active site: G79 (≠ A68), E100 (= E89), R104 (≠ A93), G127 (= G116), E130 (= E119), P151 (= P138), E152 (= E139), G160 (= G147), S452 (= S429), H473 (= H450), E485 (= E462), S523 (≠ N500)
- binding adenosine-5'-diphosphate: Q640 (≠ E618), P641 (≠ Q619), P642 (≠ K620), L643 (≠ A621), Q644 (≠ F622)
- binding coenzyme a: T38 (≠ V28), V40 (≠ K30), A77 (≠ V66), G79 (≠ A68), D80 (= D69), V81 (≠ I70), E152 (= E139), F315 (≠ L294), Q319 (vs. gap)
8oqlA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-1
35% identity, 96% coverage: 7:691/716 of query aligns to 13:713/728 of 8oqlA
- binding Hexafluorophosphate anion: V37 (≠ K30), N39 (≠ D32), G75 (= G67), D77 (= D69), M81 (≠ F73), V92 (≠ L84), T95 (≠ W87), P148 (= P138), E149 (= E139), L152 (= L142), Q180 (≠ T169), Q260 (≠ A242), K362 (= K342), D363 (= D343), V364 (≠ I344), V430 (= V410), D476 (≠ H456), K477 (= K457), M478 (= M458), P479 (= P459), K506 (= K486)
- binding formamide: V78 (≠ I70), K79 (≠ T71)
8oqrA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-80
35% identity, 96% coverage: 7:691/716 of query aligns to 14:714/728 of 8oqrA
- binding 4-cyanobenzenesulfonic acid: G76 (= G67), G77 (≠ A68), T81 (≠ E72), M82 (≠ F73), M82 (≠ F73), A85 (≠ L76), D89 (= D80), T96 (≠ W87), L123 (= L114), G124 (= G115), P149 (= P138), E150 (= E139), S366 (≠ A345), L367 (≠ Q346), E368 (≠ P347), A420 (= A399), V421 (= V400), F422 (≠ V401)
8oqsB Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-83
35% identity, 96% coverage: 7:691/716 of query aligns to 20:720/735 of 8oqsB
- binding 4-phenylbenzenesulfonic acid: M45 (≠ F31), I50 (≠ L36), G82 (= G67), G83 (≠ A68), D84 (= D69), T87 (≠ E72), T87 (≠ E72), M88 (≠ F73), M88 (≠ F73), A91 (≠ L76), D95 (= D80), D98 (≠ V83), V99 (≠ L84), T102 (≠ W87), T102 (≠ W87), E134 (= E119), E156 (= E139), L159 (= L142), F302 (= F277), F302 (= F277), F319 (≠ L294), S456 (= S429), T457 (= T430), M485 (= M458), P486 (= P459), G523 (= G496), S527 (≠ N500), N535 (≠ A508), P560 (= P534), M575 (≠ G549), I578 (≠ A552), I578 (≠ A552), I682 (≠ V659), M683 (≠ Y660), G686 (= G663)
Sites not aligning to the query:
8oqoA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-49
35% identity, 96% coverage: 7:691/716 of query aligns to 13:713/727 of 8oqoA
8oqqA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-79
35% identity, 96% coverage: 7:691/716 of query aligns to 8:708/723 of 8oqqA
8oqpA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-76
35% identity, 96% coverage: 7:691/716 of query aligns to 8:708/723 of 8oqpA
- binding 2-azanyl-5-sulfo-benzoic acid: G28 (= G26), S29 (= S27), A63 (≠ G60), K64 (= K61), K64 (= K61), K65 (≠ D62), P143 (= P138), E144 (= E139), L147 (= L142), F307 (≠ L294), M473 (= M458), P548 (= P534), S599 (≠ L581), L602 (≠ K584), K603 (≠ N585), S661 (= S650), T662 (≠ P651), G674 (= G663), A698 (= A681), R705 (≠ D688)
8pf8A Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-72
35% identity, 96% coverage: 7:691/716 of query aligns to 14:714/729 of 8pf8A
- binding [2-methyl-5-(trifluoromethyl)pyrazol-3-yl]boronic acid: G76 (= G67), F169 (≠ I158), N173 (= N162), S177 (≠ E165), I193 (≠ V181), F313 (≠ L294)
- binding bis[2-methyl-5-(trifluoromethyl)pyrazol-3-yl]-bis(oxidanyl)boranuide: M39 (≠ F31), N40 (≠ D32), E41 (≠ R33), T81 (≠ E72), D92 (≠ V83), V93 (≠ L84)
- binding [(2~{R})-2,3-bis(oxidanyl)propoxy]-[2-methyl-5-(trifluoromethyl)pyrazol-3-yl]borinic acid: M39 (≠ F31), G77 (≠ A68), D78 (= D69), M82 (≠ F73), V93 (≠ L84)
- binding (2~{R})-3-bis[2-methyl-5-(trifluoromethyl)pyrazol-3-yl]boranyloxypropane-1,2-diol: T152 (≠ K141), R184 (≠ D172), A311 (≠ I292), F312 (= F293), I673 (≠ M656)
Sites not aligning to the query:
8oquA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-92
35% identity, 96% coverage: 7:691/716 of query aligns to 15:715/730 of 8oquA
- binding 4-chloranylbenzenesulfonic acid: M40 (≠ F31), N41 (≠ D32), E42 (≠ R33), G77 (= G67), G78 (≠ A68), D79 (= D69), V80 (≠ I70), D90 (= D80), V94 (≠ L84), L124 (= L114), G125 (= G115), P150 (= P138), E151 (= E139)
8oqtA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-91
35% identity, 96% coverage: 7:691/716 of query aligns to 14:714/729 of 8oqtA
- binding 4-bromanylbenzenesulfonic acid: E41 (≠ R33), G76 (= G67), G77 (≠ A68), D78 (= D69), V79 (≠ I70), M82 (≠ F73), D89 (= D80), V93 (≠ L84), T96 (≠ W87), T96 (≠ W87), P149 (= P138), E150 (= E139)
Sites not aligning to the query:
8oqnA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-53
35% identity, 96% coverage: 7:691/716 of query aligns to 14:714/729 of 8oqnA
- binding 1-benzyl-1H-pyrazole-4-carboxylic acid: M39 (≠ F31), M82 (≠ F73), E150 (= E139), Q172 (≠ D161), F175 (vs. gap), V176 (≠ L164), Q181 (≠ T169), T241 (= T223), F254 (= F233), N257 (≠ G238), Q261 (≠ A242), L262 (≠ V243), P266 (≠ H247), P268 (= P249), Q282 (≠ T263), V283 (≠ K264), G302 (≠ T283), Q303 (≠ D284), V304 (= V285), S521 (≠ N500), G525 (≠ F504)
8opvA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with resveratrol (fragment-b-h11)
35% identity, 96% coverage: 7:691/716 of query aligns to 14:714/729 of 8opvA
- binding resveratrol: M39 (≠ F31), A75 (≠ V66), G76 (= G67), M82 (≠ F73), E128 (= E119), P149 (= P138), E150 (= E139), T152 (≠ K141), L153 (= L142), R184 (≠ D172), F296 (= F277)
8opuA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with sulfamethoxazole (fragment-b-e1)
35% identity, 96% coverage: 7:691/716 of query aligns to 14:714/729 of 8opuA
Query Sequence
>7022769 FitnessBrowser__ANA3:7022769
MIYQSPTIQVELLEDNIAKLCFNAPGSVNKFDRETLASLDAALDSIKQQSNIQALVLTSG
KDTFIVGADITEFLGLFAQDDAVLLSWIEQANAVFNKLEDLPFPTASAIKGFALGGGCET
ILATDFRIADTTAKIGLPETKLGIIPGFGGTVRLPRVIGADNALEWITTGKDQRPEDALK
VGAVDAVVAPEALEAAAIQMLKDAVAEKLDWQARRQRKMSPLTLPKLEAMMSFTTAKGMV
FAVAGKHYPAPMAAVSVVEQAATKGRSDALQIEHQAFIKLAKTDVAKALIGIFLNDQLVK
GKAKKAGKLAKDVKSAAVLGAGIMGGGIAYQSASKGTPIVMKDIAQPALDLGLGEAAKLL
SAQVARGRSTPEKMAKVLNNITPALDYAPVKHADVVVEAVVEHPKVKAQVLAEVEQYVSE
DAIIASNTSTISINLLAKSMKKPERFCGMHFFNPVHKMPLVEVIRGEHSSEETIASVVAY
ASKMGKTPIVVNDCPGFFVNRVLFPYFAGFNGLLAEGGDFAAIDKVMEKQFGWPMGPAYL
LDVVGLDTGHHAQAVMAEGFPDRMGKSGNDAIDVMFENKRLGQKNGKGFYAYSVDSRGKP
KKDVDPTSYELLKAAFGEQKAFDADEIIARTMIPMIIETVRCLEEGIVASPAEADMGLVY
GLGFPPFRGGVFRYLDTMGVANFVALADKYAHLGGLYQVTDAMRALAANNGSYYQA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory