SitesBLAST

6u9cA The 2.2 a crystal structure of the type b chloramphenicol acetyltransferase from vibrio cholerae in the complex with acetyl coa
45% identity, 88% coverage: 14:202/215 of query aligns to 13:199/206 of 6u9cA

query
sites
6u9cA

L

L

H

D

Q

Q

V

Q

V

V

T

T

N

N

P

P

N

N

I

I

T

-

I

I

K

V

G

G

E

K

H

H

S

S

Y

Y

S

S

D

G

C

Y

W

Y

D

H

K

G

G

H

F

S

E

F

E

D

S

D

V

C

V

V

R

R

Y

Y

L

L

Y

H

G

P

D

E

E

R

I

D

S

-

L

-

T

-

W

-

D

-

P

-

L

-

W

-

E

D

I

V

D

D

K

K

L

L

H

V

I

I

G

G

D

S

Y

F

V

C

C

S

I

I

G

G

A

S

E

G

A

A

V

V

I

F

L

M

M

M

G

A

G

G

N

N

H

Q

T

G

H

H

R

R

A

S

D

D

W

W

F

I

C

S

L

T

Y

F

P

P

F

F

M

F

-

Y

-

Q

-

D

-

N

D

D

V

N

I

F

E

A

E

D

A

A

-

R

-

D

-

G

Y

F

V

T

G

R

K

S

G

G

D

D

T

T

H

I

I

I

G

G

D

H

G

D

A

V

W
|
W

L

I

G

G

M

T

R

E

A

A

M

M

I

I

M

M

P

P

G

G

V

V

S

K

I

I

G

G

E

H

G

G

A

A

I

I

V

I

A
|
A

A

S

N

R

S

S

V

V

T

T

Q

K

D

D

V

V

A

A

P

P

Y

Y

S

E

I

V

V

V

G

G

G

S

T

N

P

P

A

A

K

K

L

H

V
x
I

K

K

Y

F

R

R

F

F

E

S

P

D

S

V

V

E

I

I

D

A

E

M

L

L

G

E

L

M

R

A

I

W

Y

W

D

N

W

W

P

P

Q

E

A

S

K

W

F

L

E

K

S

E

L

S

R

M

R

Q

Y

S

L

L

C

C

A

S

S

S

D

D

I

I

E

E

K

G

L

L

binding acetyl coenzyme *a: W116 (= W119), A136 (= A139), I159 (≠ V162)

6pxaE The crystal structure of chloramphenicol acetyltransferase-like protein from vibrio fischeri es114 in complex with taurocholic acid
41% identity, 92% coverage: 8:204/215 of query aligns to 10:213/221 of 6pxaE

query
sites
6pxaE

W

W

S
x
L

K
x
E

V
x
G

Q

Q

L

N

L

L

H

K

Q

E

V

Q

V

V

T

K

N

N

P

P

N

N

I

I

T

E

I

V

K

-

G

G

E

D

H

Y

S

S

Y
|
Y

Y

Y

S

S

D

G

C

F

W

Y

-

H

D

S

K

K

G

T

F

F

E

E

S

Q

V

A

V

V

R

R

Y

Y

L

L

Y

L

G

G

D

D

E

A

I

P

S

T

L

Q

-

E

T

V

W

W

D

E

P

S

-

G

-

Q

L
x
F

W

G

E

E

I

V

D

D

K

K

L

L

H

R

I

I

G

G

D

K

Y

F

V

C

C

S

I

I

G

A

A

S

E

G

A

A

V

T

I

F

L

M

M

M

G

A

G

G

N

N

H
x
Q

T

G

H

H

R

R

A

A

D

D

W

W

F

I

C

S

L

T

Y
x
F

P
|
P

F
|
F

M
x
S

-

K

-

E

-
x
F

-

G

D

E

V

G

I

V

E

K

E

D

A

G

Y

F

V

Q

G

R

K

A

G

G

D

D

T

T

H

I

I

V

G

G

D

N

G

D

A

V

W

W

L

I

G

G

M

S

R

E

A

A

M

M

I

I

M

M

P

P

G

G

V

V

S

H

I

I

G

G

E

D

G

G

A

A

I

I

V

I

A

G

A

A

N

R

S

A

V

V

V

I

T

T

Q

K

D

N

V

V

A

A

P

P

Y

Y

S

S

I

V

V

V

G

-

G

V

T

G

P

N

A

N

K

V

L

V

V

V

K

K

Y

K

R

R

F

F

E

D

P

E

S

N

V

L

I

I

D

Q

E

T

L

L

G

V

L

I

R

K

I

W

Y

W

D

D

W

W

P

P

Q

L

A

Q

K

H

F

I

E

K

S

N

L

T

R

M

R

E

Y

I

L

L

C

C

A

S

S

G

D

H

I

I

E

E

K

E

L

L

K

E

E

Q

binding taurocholic acid: L11 (≠ S9), E12 (≠ K10), G13 (≠ V11), Y32 (= Y31), F65 (≠ L60), Q91 (≠ H86), F101 (≠ Y96), P102 (= P97), F103 (= F98), S104 (≠ M99), F108 (vs. gap)

5ux9A The crystal structure of chloramphenicol acetyltransferase from vibrio fischeri es114
41% identity, 92% coverage: 8:204/215 of query aligns to 5:208/215 of 5ux9A

query
sites
5ux9A

W

W

S

L

K

E

V

G

Q

Q

L

N

L

L

H

K

Q

E

V

Q

V

V

T

K

N

N

P

P

N

N

I

I

T

E

I

V

K

-

G

G

E

D

H

Y

S

S

Y

Y

S

S

D

G

C

F

W

Y

-

H

D

S

K

K

G

T

F

F

E

E

S

Q

V

A

V

V

R

R

Y

Y

L

L

Y

L

G

G

D

D

E

A

I

P

S

T

L

Q

-

E

T

V

W

W

D

E

P

S

-

G

-

Q

L

F

W

G

E

E

I

V

D

D

K

K

L

L

H

R

I

I

G

G

D

K

Y

F

V

C

C

S

I

I

G

A

A

S

E

G

A

A

V

T

I

F

L

M

M

M

G

A

G

G

N

N

H

Q

T

G

H

H

R

R

A

A

D

D

W

W

F

I

C

S

L

T

Y

F

P

P

F

F

M

S

-

K

-

E

-

F

-

G

D

E

V

G

I

V

E

K

E

D

A

G

Y

F

V

Q

G

R

K

A

G

G

D

D

T

T

H

I

I

V

G

G

D

N

G

D

A

V

W

W

L

I

G

G

M

S

R

E

A

A

M

M

I

I

M

M

P

P

G

G

V

V

S

H

I

I

G

G

E

D

G

G

A

A

I

I

V

I

A

G

A

A

N

R

S

A

V

V

V

I

T

T

Q

K

D

N

V

V

A

A

P

P

Y

Y

S

S

I

V

V

V

G

-

G

V

T

G

P

N

A

N

K

V

L

V

V

V

K

K

Y

K

R

R

F

F

E

D

P

E

S

N

V

L

I

I

D

Q

E

T

L

L

G

V

L

I

R

K

I

W

Y

W

D

D

W

W

P

P

Q

L

A

Q

K

H

F

I

E

K

S

N

L

T

R

M

R

E

Y

I

L

L

C

C

A

S

S

G

D
x
H

I

I

E

E

K
x
E

L

L

K

E

E

Q

binding magnesium ion: H202 (≠ D198), E205 (≠ K201)

2xatA Complex of the hexapeptide xenobiotic acetyltransferase with chloramphenicol and desulfo-coenzyme a (see paper)
38% identity, 91% coverage: 10:204/215 of query aligns to 8:201/208 of 2xatA

query
sites
2xatA

K
x
R

V

G

Q

K

L

L

H

S

Q

E

V

Q

V

V

T

S

N

N

P

P

N

N

I

I

T

R

I

V

K

G

G

R

E

Y

H

S

S
x
Y

Y

Y

Y

S

S

G

D

Y

C

Y

W

H

D

G

K

H

G

S

F

F

E

D

S

-

V

C

V

A

R

R

Y

Y

L

L

Y

M

G

P

D

D

E

R

I

D

S

-

L

-

T

-

W

-

D

-

P

-

L

-

W

-

E

D

I

V

D

D

K

K

L

L

H

V

I

I

G

G

D

S

Y

F

V

C

C

S

I

I

G

G

A

S

E

G

A

A

V

A

I

F

L

I

M

M

G

A

G

G

N

N

H

Q

T

G

H

H

R

R

A

A

D

E

W

W

F

A

C

S

L

T

Y

F

P

P

F

F

M

H

D

F

V

M

I

H

E

E

-

P

-

A

-

F

-

A

-

G

-

A

-

V

-

N

A

G

Y

Y

V

Q

G

P

K

A

G

G

D

D

T

T

H

L

I

I

G

G

D

H

G

E

A

V

W

W

L

I

G
|
G

M
x
T

R

E

A

A

M

M

I

F

M

M

P

P

G

G

V

V

S

R

I

V

G

G

E

H

G

G

A

A

I

I

V

I

A
x
G

A
x
S

N

R

S

A

V

L

V

V

T

T

Q

G

D

D

V

V

A

E

P

P

Y

Y

S

A

I

I

V

V

G
|
G

T

N

P

P

A

A

K

R

L

T

V
x
I

K

R

Y

K

R

R

F

F

E

S

P

D

S

G

V

D

I

I

D

Q

E

N

L

L

G

E

L

M

R

A

I

W

Y

W

D

D

W

W

P

P

Q

L

A

A

K

D

F

I

E

E

S

A

L

A

R

M

R

P

Y

L

L

L

C

C

A

T

S

G

D

D

I

I

E

P

K

A

L

L

K

Y

E

Q

binding chloramphenicol: R8 (≠ K10), Y28 (≠ S30)
binding desulfo-coenzyme a: G118 (= G121), T119 (≠ M122), G136 (≠ A139), S137 (≠ A140), G152 (= G155), G153 (= G156), I159 (≠ V162)

Sites not aligning to the query:

binding chloramphenicol: 6, 7

4hurA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with acetyl coenzyme a (see paper)
37% identity, 93% coverage: 10:210/215 of query aligns to 16:211/211 of 4hurA

query
sites
4hurA

K

N

V

L

Q

Q

L

F

L

I

H

K

Q

P

V

T

V

I

T

T

N

N

P

E

N

N

I

I

T

L

I

V

K

-

G

G

E

E

H

Y

S

S

Y

Y

S

D

D

S

C

K

W

R

D

G

K

E

G

S

F

F

E

E

E

D

S

Q

V

V

V

-

R

-

Y

-

L

L

Y
|
Y

G

H

D

Y

E

E

I

V

S

I

L

G

T

-

W

-

D

-

P

-

L

-

W

-

E

-

I

-

D

D

K

K

L

L

H

I

I

I

G

G

D

R

Y

F

V

C

C
x
S

I
|
I

G
|
G

A

P

E

G

A

T

V

T

I

F

L

I

M

M

G

N

G

G

N
x
A

H
x
N

T

-

H

H

R

R

A

M

D

D

W

G

F

-

C

S

L

T

Y

Y

P

P

F

F

-

H

-

L

-

F

-

R

-

M

-

G

-

W

-

E

-

K

-

Y

M

M

D

P

V

S

I

L

E

K

E

D

A

L

Y

P

V

L

G

-

K
|
K

G

G

D

D

T

I

H

E

I

I

G

G

D

N

G

D

A

V

W
|
W

L
x
I

G
|
G

M
x
R

R

D

A

V

M

T

I

I

M
|
M

P

P

G

G

V

V

S

K

I

I

G

G

E

D

G

G

A

A

I

I

V

I

A
|
A

N

E

S

A

V

V

T
|
T

Q

K

D

N

V

V

A

A

P

P

Y

Y

S

S

I

I

V

V

G
|
G

G

G

T

N

P
|
P

A

L

K

K

L

F

V
x
I

K
x
R

Y

K

R

R

F

F

E

S

P

D

S

G

V

V

I

I

D

E

E

E

L

W

L

L

G

A

L

L

R

Q

I

W

Y

W

D

N

W

L

P

D

Q

M

A

K

K

I

F

I

E

N

S

E

L

N

R

L

R

P

Y

F

L

I

C

I

A

N

S

G

D

D

I

I

E

E

K

M

L

L

K

K

E

R

A

K

A

R

L

K

A

L

Y

L

D

D

binding acetyl coenzyme *a: Y51 (= Y49), S67 (≠ C73), I68 (= I74), G69 (= G75), A79 (≠ N85), N80 (≠ H86), K110 (= K109), W120 (= W119), I121 (≠ L120), G122 (= G121), R123 (≠ M122), M128 (= M127), A140 (= A139), A141 (= A140), T146 (= T145), G156 (= G155), P159 (= P158), I163 (≠ V162), R164 (≠ K163)

4husA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with virginiamycin m1 (see paper)
37% identity, 93% coverage: 10:210/215 of query aligns to 16:211/212 of 4husA

query
sites
4husA

K

N

V
x
L

Q

Q

L

F

L

I

H

K

Q

P

V

T

V

I

T

T

N

N

P

E

N

N

I

I

T

L

I

V

K

-

G

G

E

E

H

Y

S

S

Y
|
Y

Y

Y

S
x
D

D

S

C

K

W

R

D

G

K

E

G

S

F

F

E

E

E

D

S

Q

V

V

V

-

R

-

Y

-

L

L

Y

Y

G

H

D

Y

E

E

I

V

S

I

L

G

T

-

W

-

D

-

P

-

L

-

W

-

E

-

I

-

D

D

K

K

L

L

H

I

I

I

G

G

D

R

Y

F

V

C

C

S

I

I

G

G

A

P

E

G

A

T

V

T

I

F

L

I

M

M

G

N

G

G

N

A

H

N

T

-

H

H

R

R

A

M

D

D

W

G

F

-

C

S

L

T

Y

Y

P

P

F

F

-

H

-

L

-

F

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R

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M

-

G

-

W

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E

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K

-

Y

M

M

D

P

V

S

I

L

E

K

E

D

A

L

Y

P

V

L

G

-

K

K

G

G

D

D

T

I

H

E

I

I

G

G

D

N

G

D

A

V

W

W

L

I

G

G

M

R

R

D

A

V

M

T

I

I

M

M

P

P

G

G

V

V

S

K

I

I

G

G

E

D

G

G

A

A

I

I

V

I

A

A

N

E

S

A

V

V

T

T

Q

K

D

N

V

V

A

A

P

P

Y

Y

S

S

I

I

V

V

G

G

T

N

P

P

A

L

K

K

L

F

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K

R

Y

K

R

R

F

F

E

S

P

D

S

G

V

V

I

I

D

E

E

E

L

W

L

L

G

A

L

L

R

Q

I

W

Y

W

D

N

W

L

P

D

Q

M

A

K

K

I

F

I

E

N

S

E

L

N

R

L

R

P

Y

F

L

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C

I

A

N

S

G

D

D

I

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E

E

K

M

L

L

K

K

E

R

A

K

A

R

L

K

A

L

Y

L

D

D

binding virginiamycin m1: L17 (≠ V11), Y36 (= Y31), D38 (≠ S33)

6x3jA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f0224 (46) (see paper)
38% identity, 91% coverage: 10:204/215 of query aligns to 16:205/206 of 6x3jA

query
sites
6x3jA

K

N

V

L

Q

Q

L

F

L

I

H

K

Q

P

V

T

V

I

T

T

N

N

P

E

N

N

I

I

T

L

I

V

K

-

G

G

E

E

H

Y

S

S

Y

Y

S

D

D

S

C

K

W

R

D

G

K

E

G

S

F

F

E

E

E

D

S

Q

V

V

V

-

R

-

Y

-

L

L

Y
|
Y

G

H

D
x
Y

E

E

I
x
V

S

I

L

G

T

-

W

-

D

-

P

-

L

-

W

-

E

-

I

-

D

D

K

K

L

L

H

I

I

I

G

G

D

R

Y

F

V

C

C

S

I

I

G
|
G

A

P

E

G

A

T

V

T

I

F

L

I

M

M

G

N

G

G

N
x
A

H

N

T

-

H
|
H

R

R

A

M

D

D

W

G

F

-

C

S

L

T

Y

Y

P

P

F

F

-
x
H

-
x
L

-

F

-

R

-

M

-

G

-

W

-

E

-

K

-

Y

M
|
M

D
x
P

V

S

I

L

E

K

E

D

A
x
L

Y

P

V

L

G

-

K

K

G

G

D

D

T

I

H

E

I

I

G

G

D

N

G

D

A

V

W
|
W

L

I

G
|
G

M

R

R

D

A

V

M

T

I

I

M

M

P

P

G

G

V

V

S

K

I

I

G

G

E

D

G

G

A

A

I

I

V

I

A

A

N

E

S

A

V

V

T

T

Q

K

D

N

V

V

A

A

P

P

Y

Y

S

S

I

I

V

V

G

G

T

N

P

P

A

L

K

K

L

F

V

I

K

R

Y

K

R

R

F

F

E

S

P

D

S

G

V

V

I

I

D

E

E

E

L

W

L

L

G

A

L

L

R

Q

I

W

Y

W

D

N

W

L

P

D

Q

M

A

K

K

I

F

I

E

N

S

E

L

N

R

L

R

P

Y

F

L

I

C

I

A

N

S

G

D

D

I

I

E

E

K

M

L

L

K

K

E

R

binding (2R)-2-[(3S,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-4,12-dimethyl-1,7,22-trioxo-4,7,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,3H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosin-3-yl]propyl isoquinolin-3-ylcarbamate: Y51 (= Y49), Y53 (≠ D51), V55 (≠ I53), H91 (vs. gap), L92 (vs. gap), M101 (= M99), P102 (≠ D100), L107 (≠ A105)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: G69 (= G75), A79 (≠ N85), H81 (= H88), W120 (= W119), G122 (= G121)

6x3cA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
38% identity, 91% coverage: 10:204/215 of query aligns to 16:205/207 of 6x3cA

query
sites
6x3cA

K

N

V
x
L

Q

Q

L

F

L

I

H

K

Q

P

V

T

V

I

T

T

N

N

P

E

N

N

I

I

T

L

I

V

K

-

G

G

E

E

H

Y

S

S

Y
|
Y

Y

Y

S

D

D

S

C

K

W

R

D

G

K

E

G

S

F

F

E

E

E

D

S

Q

V

V

V

-

R

-

Y

-

L

L

Y
|
Y

G

H

D
x
Y

E

E

I

V

S

I

L

G

T

-

W

-

D

-

P

-

L

-

W

-

E

-

I

-

D

D

K

K

L

L

H

I

I

I

G

G

D

R

Y

F

V

C

C

S

I

I

G
|
G

A

P

E

G

A

T

V

T

I

F

L

I

M

M

G

N

G
|
G

N

A

H
x
N

T

-

H
|
H

R

R

A

M

D

D

W

G

F

-

C

S

L

T

Y

Y

P

P

F

F

-
x
H

-
x
L

-

F

-

R

-

M

-

G

-

W

-

E

-

K

-

Y

M
|
M

D
x
P

V

S

I
x
L

E

K

E

D

A

L

Y

P

V

L

G

-

K

K

G

G

D

D

T

I

H

E

I

I

G

G

D

N

G

D

A

V

W
|
W

L

I

G
|
G

M
x
R

R

D

A

V

M

T

I

I

M
|
M

P

P

G

G

V

V

S

K

I

I

G

G

E

D

G

G

A

A

I

I

V

I

A
|
A

N

E

S

A

V

V

T

T

Q

K

D

N

V

V

A

A

P

P

Y

Y

S

S

I

I

V

V

G

G

T

N

P

P

A

L

K

K

L

F

V

I

K

R

Y

K

R

R

F

F

E

S

P

D

S

G

V

V

I

I

D

E

E

E

L

W

L

L

G

A

L

L

R

Q

I

W

Y

W

D

N

W

L

P

D

Q

M

A

K

K

I

F

I

E

N

S

E

L

N

R

L

R

P

Y

F

L

I

C

I

A

N

S

G

D

D

I

I

E

E

K

M

L

L

K

K

E

R

binding (3R,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-en-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione: L17 (≠ V11), Y36 (= Y31), Y53 (≠ D51), G78 (= G84), N80 (≠ H86), H81 (= H88), H91 (vs. gap), L92 (vs. gap), M101 (= M99), P102 (≠ D100), L104 (≠ I102)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: Y51 (= Y49), G69 (= G75), N80 (≠ H86), H81 (= H88), W120 (= W119), G122 (= G121), R123 (≠ M122), M128 (= M127), A140 (= A139), A141 (= A140)

6x3cE Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
37% identity, 90% coverage: 10:202/215 of query aligns to 16:203/203 of 6x3cE

query
sites
6x3cE

K

N

V

L

Q

Q

L

F

L

I

H

K

Q

P

V

T

V

I

T

T

N

N

P

E

N

N

I

I

T

L

I

V

K

-

G

G

E

E

H

Y

S

S

Y

Y

S

D

D

S

C

K

W

R

D

G

K

E

G

S

F

F

E

E

E

D

S

Q

V

V

V

-

R

-

Y

-

L

L

Y
|
Y

G

H

D
x
Y

E

E

I

V

S

I

L

G

T

-

W

-

D

-

P

-

L

-

W

-

E

-

I

-

D

D

K

K

L

L

H

I

I

I

G

G

D

R

Y

F

V

C

C

S

I

I

G
|
G

A

P

E

G

A

T

V

T

I

F

L

I

M

M

G

N

G
|
G

N

A

H
x
N

T

-

H
|
H

R

R

A
x
M

D

D

W

G

F

-

C

S

L

T

Y

Y

P

P

F

F

-
x
H

-
x
L

-

F

-

R

-

M

-

G

-

W

-

E

-

K

-

Y

M
|
M

D
x
P

V

S

I

L

E

K

E

D

A

L

Y

P

V

L

G

-

K

K

G

G

D

D

T

I

H

E

I

I

G

G

D

N

G

D

A

V

W
|
W

L

I

G
|
G

M

R

R

D

A

V

M

T

I

I

M

M

P

P

G

G

V

V

S

K

I

I

G

G

E

D

G

G

A

A

I

I

V

I

A
|
A

N

E

S

A

V

V

T

T

Q

K

D

N

V

V

A

A

P

P

Y

Y

S

S

I

I

V

V

G

G

T
x
N

P
|
P

A

L

K

K

L

F

V

I

K

R

Y

K

R

R

F

F

E

S

P

D

S

G

V

V

I

I

D

E

E

E

L

W

L

L

G

A

L

L

R

Q

I

W

Y

W

D

N

W

L

P

D

Q

M

A

K

K

I

F

I

E

N

S

E

L

N

R

L

R

P

Y

F

L

I

C

I

A

N

S

G

D

D

I

I

E

E

K

M

L

L

binding magnesium ion: N158 (≠ T157), P159 (= P158)
binding (3R,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-en-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione: Y53 (≠ D51), G78 (= G84), N80 (≠ H86), H81 (= H88), H91 (vs. gap), L92 (vs. gap), M101 (= M99), P102 (≠ D100)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: Y51 (= Y49), G69 (= G75), H81 (= H88), M83 (≠ A90), W120 (= W119), G122 (= G121), A140 (= A139), A141 (= A140)

3dhoA Structure of streptogramin acetyltransferase in complex with an inhibitor
40% identity, 73% coverage: 27:183/215 of query aligns to 33:185/203 of 3dhoA

query
sites
3dhoA

G

G

E

E

H

Y

S

S

Y

Y

S

D

D

S

C

K

W

N

D

G

K

E

G

T

F

F

E

D

E

K

S

Q

V

I

V

L

R

-

Y

Y

L

H

Y

Y

G

-

D

-

E

-

I

-

S

-

L

-

T

-

W

-

D

-

P

P

L

I

W

L

E

N

I

-

D

D

K

K

L

L

H

K

I

I

G

G

D

K

Y

F

V

C

C

S

I

I

G

G

A

P

E

G

A

V

V

T

I

I

L

I

M

M

G

N

G

G

N

-

H

A

T

N

H

H

R

R

A
x
M

D

D

W

G

F

-

C

S

L

T

Y

Y

P

P

F

F

-

N

-

L

-

F

-

G

-

N

-

G

-

W

-

E

-

K

-

H

M

M

D

P

V

K

I

L

E

D

E

Q

A

L

Y

P

V

I

G

-

K

K

G

G

D

D

T

T

H

I

I

I

G

G

D

N

G

D

A

V

W

W

L

I

G

G

M

K

R

D

A

V

M

V

I

I

M

M

P

P

G

G

V

V

S

K

I

I

G

G

E

D

G

G

A

A

I
|
I

V

V

A
|
A

A

A

N

N

S

S

V

V

T
x
V

Q

K

D

D

V

I

A

A

P

P

Y

Y

S

M

I
x
L

V

A

G
|
G

G

G

T

N

P
|
P

A

A

K

N

L

E

V
x
I

K

K

Y

Q

R

R

F

F

E

D

P

Q

S

D

V

T

I

I

D

N

E

Q

L

L

G

D

L

I

R

K

I

W

Y

W

D

N

W

W

P

P

binding 6-bromo-N'-[(1Z)-(3,5-dichloro-2-hydroxyphenyl)methylidene]-2-methylquinoline-4-carbohydrazide: M84 (≠ A90), I139 (= I137), A141 (= A139), V147 (≠ T145), L155 (≠ I153), G157 (= G155), P160 (= P158), I164 (≠ V162)

1mrlA Crystal structure of streptogramin a acetyltransferase with dalfopristin (see paper)
40% identity, 73% coverage: 27:183/215 of query aligns to 33:185/204 of 1mrlA

query
sites
1mrlA

G

G

E

E

H

Y

S

S

Y
|
Y

Y

Y

S
x
D

D

S

C

K

W

N

D

G

K

E

G

T

F

F

E

D

E

K

S

Q

V

I

V

L

R

-

Y

Y

L

H

Y
|
Y

G

-

D

-

E

-

I

-

S

-

L

-

T

-

W

-

D

-

P

P

L
x
I

W

L

E

N

I

-

D

D

K

K

L

L

H

K

I

I

G

G

D

K

Y

F

V

C

C

S

I

I

G

G

A

P

E

G

A

V

V

T

I

I

L

I

M

M

G

N

G

G

N

-

H

A

T
x
N

H
|
H

R

R

A

M

D

D

W

G

F

-

C

S

L

T

Y

Y

P

P

F

F

-

N

-
x
L

-

F

-

G

-

N

-

G

-

W

-

E

-

K

-

H

M
|
M

D

P

V

K

I

L

E

D

E

Q

A
x
L

Y

P

V

I

G

-

K

K

G

G

D

D

T

T

H

I

I

I

G

G

D

N

G

D

A

V

W

W

L

I

G

G

M

K

R

D

A

V

M

V

I

I

M

M

P

P

G

G

V

V

S

K

I

I

G

G

E

D

G

G

A

A

I

I

V

V

A

A

N

N

S

S

V

V

T

V

Q

K

D

D

V

I

A

A

P

P

Y

Y

S

M

I

L

V

A

G

G

T

N

P

P

A

A

K

N

L

E

V

I

K

K

Y

Q

R

R

F

F

E

D

P

Q

S

D

V

T

I

I

D

N

E

Q

L

L

G

D

L

I

R

K

I

W

Y

W

D

N

W

W

P

P

binding 5-(2-diethylamino-ethanesulfonyl)-21-hydroxy-10-isopropyl-11,19-dimethyl-9,26-dioxa-3,15,28-triaza-tricyclo[23.2.1.00,255]octacosa-1(27),12,17,19,25(28)-pentaene-2,8,14,23-tetraone: Y37 (= Y31), D39 (≠ S33), Y54 (= Y49), I56 (≠ L60), N81 (≠ T87), H82 (= H88), L93 (vs. gap), M102 (= M99), L108 (≠ A105)

1khrA Crystal structure of vat(d) in complex with virginiamycin and coenzyme a (see paper)
40% identity, 73% coverage: 27:183/215 of query aligns to 33:185/206 of 1khrA

query
sites
1khrA

G

G

E

E

H

Y

S

S

Y
|
Y

Y

Y

S
x
D

D

S

C

K

W

N

D

G

K

E

G

T

F

F

E

D

E

K

S

Q

V

I

V

L

R

-

Y

Y

L

H

Y
|
Y

G

-

D

-

E

-

I

-

S

-

L

-

T

-

W

-

D

-

P

P

L

I

W

L

E

N

I

-

D

D

K

K

L

L

H

K

I

I

G

G

D

K

Y

F

V

C

C

S

I

I

G

G

A

P

E

G

A

V

V

T

I

I

L

I

M

M

G

N

G

G

N

-

H
x
A

T
x
N

H
|
H

R

R

A
x
M

D

D

W

G

F

-

C

S

L

T

Y

Y

P

P

F

F

-

N

-
x
L

-

F

-

G

-

N

-

G

-

W

-

E

-

K

-

H

M

M

D

P

V

K

I

L

E

D

E

Q

A

L

Y

P

V

I

G

-

K

K

G

G

D

D

T

T

H

I

I

I

G

G

D

N

G

D

A

V

W
|
W

L

I

G

G

M

K

R

D

A

V

M

V

I

I

M

M

P

P

G

G

V

V

S

K

I

I

G

G

E

D

G

G

A

A

I

I

V

V

A
|
A

N

N

S

S

V

V

T
x
V

Q

K

D

D

V

I

A

A

P

P

Y

Y

S

M

I
x
L

V

A

G
|
G

G

G

T

N

P
|
P

A

A

K

N

L

E

V
x
I

K

K

Y

Q

R

R

F

F

E

D

P

Q

S

D

V

T

I

I

D

N

E

Q

L

L

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D

L

I

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K

I

W

Y

W

D

N

W

W

P

P

binding coenzyme a: H82 (= H88), M84 (≠ A90), W121 (= W119), A141 (= A139), A142 (= A140), V147 (≠ T145), L155 (≠ I153), G157 (= G155), P160 (= P158), I164 (≠ V162)
binding virginiamycin m1: Y37 (= Y31), D39 (≠ S33), Y54 (= Y49), A80 (≠ H86), N81 (≠ T87), H82 (= H88), L93 (vs. gap)

P50870 Streptogramin A acetyltransferase; Virginiamycin acetyltransferase D; Vat(D); EC 2.3.1.- from Enterococcus faecium (Streptococcus faecium) (see paper)
40% identity, 73% coverage: 27:183/215 of query aligns to 33:185/209 of P50870

query
sites
P50870

G

G

E

E

H

Y

S

S

Y

Y

S

D

D

S

C

K

W

N

D

G

K

E

G

T

F

F

E

D

E

K

S

Q

V

I

V

L

R

-

Y

Y

L

H

Y

Y

G

-

D

-

E

-

I

-

S

-

L

-

T

-

W

-

D

-

P

P

L

I

W

L

E

N

I

-

D

D

K

K

L

L

H

K

I

I

G

G

D

K

Y

F

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C

C

S

I

I

G

G

A

P

E

G

A

V

V

T

I

I

L

I

M

M

G

N

G

G

N

-

H

A

T

N

H
|
H

R

R

A

M

D

D

W

-

F

G

C

S

L

T

Y

Y

P

P

F

F

-

N

-

L

-

F

-

G

-

N

-

G

-

W

-

E

-

K

-

H

M

M

D

P

V

K

I

L

E

D

E

Q

A

L

Y

P

V

I

G

-

K

K

G

G

D

D

T

T

H

I

I

I

G

G

D

N

G

D

A

V

W

W

L

I

G

G

M

K

R

D

A

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M

V

I

I

M

M

P

P

G

G

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V

S

K

I

I

G

G

E

D

G

G

A

A

I

I

V

V

A

A

N

N

S

S

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V

T

V

Q

K

D

D

V

I

A

A

P

P

Y

Y

S

M

I

L

V

A

G

G

T

N

P

P

A

A

K

N

L

E

V

I

K

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Y

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R

F

F

E

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W

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P

H82 (= H88) mutation to A: 105-fold decrease in activity.

1kk4A Crystal structure of vat(d) in complex with acetyl-coa (see paper)
40% identity, 73% coverage: 27:183/215 of query aligns to 33:185/205 of 1kk4A

query
sites
1kk4A

G

G

E

E

H

Y

S

S

Y

Y

S

D

D

S

C

K

W

N

D

G

K

E

G

T

F

F

E

D

E

K

S

Q

V

I

V

L

R

-

Y

Y

L

H

Y

Y

G

-

D

-

E

-

I

-

S

-

L

-

T

-

W

-

D

-

P

P

L

I

W

L

E

N

I

-

D

D

K

K

L

L

H

K

I

I

G

G

D

K

Y

F

V

C

C

S

I
|
I

G
|
G

A

P

E

G

A

V

V

T

I

I

L

I

M

M

G

N

G

G

N

-

H

A

T

N

H

H

R

R

A

M

D

D

W

G

F

-

C

S

L

T

Y

Y

P

P

F

F

-

N

-

L

-

F

-

G

-

N

-

G

-

W

-

E

-

K

-

H

M

M

D

P

V

K

I

L

E

D

E

Q

A

L

Y

P

V

I

G

-

K
|
K

G

G

D

D

T

T

H

I

I

I

G

G

D

N

G

D

A

V

W
|
W

L

I

G
|
G

M
x
K

R

D

A

V

M

V

I

I

M

M

P

P

G

G

V

V

S

K

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I

G

G

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D

G

G

A

A

I

I

V

V

A
|
A

N

N

S

S

V

V

T
x
V

Q
x
K

D

D

V

I

A

A

P

P

Y

Y

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x
L

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A

G
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G

T

N

P
|
P

A

A

K

N

L

E

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x
I

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R

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binding acetyl coenzyme *a: I69 (= I74), G70 (= G75), K111 (= K109), W121 (= W119), G123 (= G121), K124 (≠ M122), A141 (= A139), A142 (= A140), V147 (≠ T145), K148 (≠ Q146), L155 (≠ I153), G157 (= G155), G158 (= G156), P160 (= P158), I164 (≠ V162)

5u2kA Crystal structure of galactoside o-acetyltransferase complex with coa (h3 space group)
43% identity, 28% coverage: 103:163/215 of query aligns to 122:182/190 of 5u2kA

query
sites
5u2kA

E

N

E

E

A

G

Y

F

V

E

G

K

K

A

G

G

D

P

T

I

H

H

I

I

G

G

D

S

G

N

A

T

W

W

L

F

G

G

M

G

R

H

A

V

M

A

I

V

M
x
L

P

P

G

G

V

V

S

T

I

I

G

G

E

E

G

G

A

S

I

V

V

I

A

G

A

A

N

G

S

S

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V

T
|
T

Q
x
K

D

D

V

I

A

P

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Y

H

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A

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N

P

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A

C

K

K

L

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R

binding coenzyme a: L146 (≠ M127), T164 (= T145), K165 (≠ Q146)

Sites not aligning to the query:

binding coenzyme a: 90, 110, 112, 114

7uujA Crystal structure of aminoglycoside resistance enzyme apma, complex with gentamicin
38% identity, 32% coverage: 114:182/215 of query aligns to 179:249/272 of 7uujA

query
sites
7uujA

I

I

G

G

D

S

G

D

A

V

W

Y

L

I

G

G

M

A

R

H

A

A

M

F

I

I

M

N

P

A

G

S

V

T

-

V

-

T

S

S

I

I

G

G

E

D

G

G

A

A

I

I

V

I

A

G

A

S

N

G

S

A

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V

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L

Q

E

D

N

V

V

A

P

P

P

Y

F

S

A

I

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V

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G

V

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P

A

A

K

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I

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K

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Y

Y

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R

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F

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K

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M

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I

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E

E

T

L

L

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D

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W

Sites not aligning to the query:

binding (2r,3r,4r,5r)-2-((1s,2s,3r,4s,6r)-4,6-diamino-3-((2r,3r,6s)-3-amino-6-(aminomethyl)-tetrahydro-2h-pyran-2-yloxy)-2-hydroxycyclohexyloxy)-5-methyl-4-(methylamino)-tetrahydro-2h-pyran-3,5-diol: 55, 85, 102, 135, 144, 145

7jm2A Crystal structure of aminoglycoside resistance enzyme apma, complex with apramycin
38% identity, 32% coverage: 114:182/215 of query aligns to 179:249/272 of 7jm2A

query
sites
7jm2A

I

I

G

G

D

S

G

D

A

V

W

Y

L

I

G

G

M

A

R

H

A

A

M

F

I

I

M

N

P

A

G

S

V

T

-

V

-

T

S

S

I

I

G

G

E

D

G

G

A

A

I

I

V

I

A

G

A

S

N

G

S

A

V

V

T

L

Q

E

D

N

V

V

A

P

P

P

Y

F

S

A

I

V

V

V

G

V

G

G

T

V

P

P

A

A

K

R

L

I

V

K

K

R

Y

Y

R

R

F

F

E

S

P

K

S

E

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M

I

I

D

E

E

T

L

L

G

R

L

V

R

K

I

W

Y

W

D

D

W

W

Sites not aligning to the query:

binding apramycin: 85, 102, 135, 144, 145, 147, 170, 172

7jm1A Crystal structure of aminoglycoside resistance enzyme apma, complex with acetyl-coa
38% identity, 32% coverage: 114:182/215 of query aligns to 179:249/272 of 7jm1A

query
sites
7jm1A

I

I

G

G

D

S

G

D

A

V

W
x
Y

L

I

G
|
G

M

A

R

H

A

A

M
x
F

I

I

M

N

P

A

G

S

V

T

-

V

-

T

S

S

I

I

G

G

E

D

G

G

A

A

I

I

V

I

A
x
G

A
x
S

N

G

S

A

V

V

T
x
L

Q

E

D

N

V

V

A

P

P

P

Y

F

S

A

I

V

V

V

G
x
V

G

G

T
x
V

P

P

A

A

K

R

L

I

V
x
K

K

R

Y

Y

R

R

F

F

E

S

P

K

S

E

V

M

I

I

D

E

E

T

L

L

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I

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Y

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D

W

W

binding acetyl coenzyme *a: Y184 (≠ W119), G186 (= G121), F190 (≠ M125), G206 (≠ A139), S207 (≠ A140), L212 (≠ T145), V222 (≠ G155), V224 (≠ T157), K229 (≠ V162)

Sites not aligning to the query:

binding acetyl coenzyme *a: 102, 124, 125, 126, 132, 134

7uukC Crystal structure of aminoglycoside resistance enzyme apma, complex with tobramycin (see paper)
38% identity, 32% coverage: 114:182/215 of query aligns to 181:251/276 of 7uukC

query
sites
7uukC

I

I

G

G

D

S

G

D

A

V

W

Y

L

I

G

G

M

A

R

H

A

A

M

F

I

I

M

N

P

A

G

S

V

T

-

V

-

T

S

S

I

I

G

G

E

D

G

G

A

A

I

I

V

I

A

G

A

S

N

G

S

A

V

V

T

L

Q

E

D

N

V

V

A

P

P

P

Y

F

S

A

I

V

V

V

G

V

G

G

T

V

P

P

A

A

K

R

L

I

V

K

K

R

Y

Y

R

R

F

F

E

S

P

K

S

E

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I

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D

E

E

T

L

L

G

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L

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I

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Y

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D

W

W

Sites not aligning to the query:

binding tobramycin: 83, 87, 104, 105, 115, 137, 146, 147

Query Sequence

>7023084 FitnessBrowser__ANA3:7023084
MLMENKHWSKVQLLHQVVTNPNITIKGEHSYYSDCWDKGFEESVVRYLYGDEISLTWDPL
WEIDKLHIGDYVCIGAEAVILMGGNHTHRADWFCLYPFMDVIEEAYVGKGDTHIGDGAWL
GMRAMIMPGVSIGEGAIVAANSVVTQDVAPYSIVGGTPAKLVKYRFEPSVIDELLGLRIY
DWPQAKFESLRRYLCASDIEKLKEAALAYDAQHCA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory