SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 7023651 Shewana3_0880 ABC transporter related (RefSeq) to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3c4jA Abc protein artp in complex with atp-gamma-s
68% identity, 100% coverage: 1:240/241 of query aligns to 3:242/242 of 3c4jA

query
sites
3c4jA

M

M

I

I

N

D

I

V

T

H

N

Q

L

L

H

K

K

K

S

S

Y
x
F

G

G

D

S

N

L

A

E

V
|
V

L

L

K

K

G

G

I

I

N

N

E

V

H

H

I

I

R

R

Q

E

G

G

E

E

V

V

V

V

S

V

V

V

I

I

G

G

P
|
P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

C

C

I

L

N

N

L

L

L

L

E

E

K

D

P

F

T

D

Q

E

G

G

D

E

I

I

E

I

I

I

E

D

G

G

Q

I

S

N

I

L

T

K

A

A

K

K

D

D

A

T

C

N

V

L

D

N

K

K

L

V

R

R

Q

E

K

E

V

V

G

G

M

M

V

V

F

F

Q

Q

N

R

F

F

N

N

L

L

F

F

P

P

H

H

K

M

T

T

V

V

L

L

Q

N

N

N

I

I

T

T

L

L

A

A

P

P

V

M

S

K

L

V

K

R

L

K

M

W

T

P

Q

R

A

E

E

K

A

A

D

E

N

A

K

K

A

A

L

M

A

E

L

L

L

L

T

D

Q

K

V

V

G

G

L

L

Q

K

D

D

K

K

A

A

N
x
H

A

A

Y
|
Y

P

P

S

D

S

S

L

L

S

S

G

G

G

G

Q

Q

K

A

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

M

M

E

E

P

P

D

K

L

I

M

M

L

L

F

F

D

D

E
|
E

P

P

T

T

S

S

A

A

L

L

D

D

P

P

E

E

M

M

V

V

G

G

D

E

V

V

L

L

D

S

V

V

M

M

K

K

D

Q

L

L

A

A

Q

N

K

E

G

G

M

M

T

T

M

M

V

V

I

V

V

V

T

T

H

H

E

E

M

M

G

G

F

F

A

A

R

R

D

E

V

V

S

G

D

D

R

R

V

V

I

L

F

F

M

M

D

D

G

G

G

G

Y

Y

V

I

V

I

E

E

S

E

N

G

I

K

P

P

E

E

E

D

L

L

F

F

T

D

R

R

P

P

K

Q

E

H

A

E

R

R

T

T

Q

K

S

A

F

F

L

L

S

S

K

K

V

V

L

F

binding phosphothiophosphoric acid-adenylate ester: F13 (≠ Y11), V18 (= V16), P37 (= P35), S38 (= S36), G39 (= G37), S40 (= S38), G41 (= G39), K42 (= K40), S43 (= S41), T44 (= T42), H133 (≠ N131), Y135 (= Y133), E164 (= E162)

3c41J Abc protein artp in complex with amp-pnp/mg2+
68% identity, 100% coverage: 1:240/241 of query aligns to 3:242/242 of 3c41J

query
sites
3c41J

M

M

I

I

N

D

I

V

T

H

N

Q

L

L

H

K

K

K

S

S

Y
x
F

G

G

D

S

N

L

A

E

V
|
V

L

L

K

K

G

G

I

I

N

N

E

V

H

H

I

I

R

R

Q

E

G

G

E

E

V

V

V

V

S

V

V

V

I

I

G

G

P
|
P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R
|
R

C

C

I

L

N

N

L

L

L

L

E

E

K

D

P

F

T

D

Q

E

G

G

D

E

I

I

E

I

I

I

E

D

G

G

Q

I

S

N

I

L

T

K

A

A

K

K

D

D

A

T

C

N

V

L

D

N

K

K

L

V

R

R

Q

E

K

E

V

V

G

G

M

M

V

V

F

F

Q

Q

N

R

F

F

N

N

L

L

F

F

P

P

H

H

K

M

T

T

V

V

L

L

Q

N

N

N

I

I

T

T

L

L

A

A

P

P

V

M

S

K

L

V

K

R

L

K

M

W

T

P

Q

R

A

E

E

K

A

A

D

E

N

A

K

K

A

A

L

M

A

E

L

L

L

L

T

D

Q

K

V

V

G

G

L

L

Q

K

D

D

K

K

A

A

N
x
H

A

A

Y
|
Y

P

P

S

D

S

S

L

L

S

S

G

G

G

G

Q

Q

K

A

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

M

M

E

E

P

P

D

K

L

I

M

M

L

L

F

F

D
|
D

E

E

P

P

T

T

S

S

A

A

L

L

D

D

P

P

E

E

M

M

V

V

G

G

D

E

V

V

L

L

D

S

V

V

M

M

K

K

D

Q

L

L

A

A

Q

N

K

E

G

G

M

M

T

T

M

M

V

V

I

V

V

V

T

T

H

H

E

E

M

M

G

G

F

F

A

A

R

R

D

E

V

V

S

G

D

D

R

R

V

V

I

L

F

F

M

M

D

D

G

G

G

G

Y

Y

V

I

V

I

E

E

S

E

N

G

I

K

P

P

E

E

E

D

L

L

F

F

T

D

R

R

P

P

K

Q

E

H

A

E

R

R

T

T

Q

K

S

A

F

F

L

L

S

S

K

K

V

V

L

F

binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ Y11), V18 (= V16), P37 (= P35), S38 (= S36), G39 (= G37), S40 (= S38), G41 (= G39), K42 (= K40), S43 (= S41), T44 (= T42), R47 (= R45), H133 (≠ N131), Y135 (= Y133)
binding magnesium ion: S43 (= S41), D163 (= D161)

2olkA Abc protein artp in complex with adp-beta-s
68% identity, 100% coverage: 1:240/241 of query aligns to 3:242/242 of 2olkA

query
sites
2olkA

M

M

I

I

N

D

I

V

T

H

N

Q

L

L

H

K

K

K

S

S

Y
x
F

G

G

D

S

N
x
L

A

E

V
|
V

L

L

K

K

G

G

I

I

N

N

E

V

H

H

I

I

R

R

Q

E

G

G

E

E

V

V

V

V

S

V

V

V

I

I

G

G

P

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

C

C

I

L

N

N

L

L

L

L

E

E

K

D

P

F

T

D

Q

E

G

G

D

E

I

I

E

I

I

I

E

D

G

G

Q

I

S

N

I

L

T

K

A

A

K

K

D

D

A

T

C

N

V

L

D

N

K

K

L

V

R

R

Q

E

K

E

V

V

G

G

M

M

V

V

F

F

Q

Q

N

R

F

F

N

N

L

L

F

F

P

P

H

H

K

M

T

T

V

V

L

L

Q

N

N

N

I

I

T

T

L

L

A

A

P

P

V

M

S

K

L

V

K

R

L

K

M

W

T

P

Q

R

A

E

E

K

A

A

D

E

N

A

K

K

A

A

L

M

A

E

L

L

L

L

T

D

Q

K

V

V

G

G

L

L

Q

K

D

D

K

K

A

A

N

H

A

A

Y

Y

P

P

S

D

S

S

L

L

S

S

G

G

G

G

Q

Q

K

A

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

M

M

E

E

P

P

D

K

L

I

M

M

L

L

F

F

D

D

E

E

P

P

T

T

S

S

A

A

L

L

D

D

P

P

E

E

M

M

V

V

G

G

D

E

V

V

L

L

D

S

V

V

M

M

K

K

D

Q

L

L

A

A

Q

N

K

E

G

G

M

M

T

T

M

M

V

V

I

V

V

V

T

T

H

H

E

E

M

M

G

G

F

F

A

A

R

R

D

E

V

V

S

G

D

D

R

R

V

V

I

L

F

F

M

M

D

D

G

G

G

G

Y

Y

V

I

V

I

E

E

S

E

N

G

I

K

P

P

E

E

E

D

L

L

F

F

T

D

R

R

P

P

K

Q

E

H

A

E

R

R

T

T

Q

K

S

A

F

F

L

L

S

S

K

K

V

V

L

F

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (≠ Y11), L16 (≠ N14), V18 (= V16), S38 (= S36), G39 (= G37), S40 (= S38), G41 (= G39), K42 (= K40), S43 (= S41), T44 (= T42)

2oljA Abc protein artp in complex with adp/mg2+
68% identity, 100% coverage: 1:240/241 of query aligns to 3:242/242 of 2oljA

query
sites
2oljA

M

M

I

I

N

D

I

V

T

H

N

Q

L

L

H

K

K

K

S

S

Y
x
F

G

G

D

S

N

L

A

E

V
|
V

L

L

K

K

G

G

I

I

N

N

E

V

H

H

I

I

R

R

Q

E

G

G

E

E

V

V

V

V

S

V

V

V

I

I

G

G

P

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

C

C

I

L

N

N

L

L

L

L

E

E

K

D

P

F

T

D

Q

E

G

G

D

E

I

I

E

I

I

I

E

D

G

G

Q

I

S

N

I

L

T

K

A

A

K

K

D

D

A

T

C

N

V

L

D

N

K

K

L

V

R

R

Q

E

K

E

V

V

G

G

M

M

V

V

F

F

Q

Q

N

R

F

F

N

N

L

L

F

F

P

P

H

H

K

M

T

T

V

V

L

L

Q

N

N

N

I

I

T

T

L

L

A

A

P

P

V

M

S

K

L

V

K

R

L

K

M

W

T

P

Q

R

A

E

E

K

A

A

D

E

N

A

K

K

A

A

L

M

A

E

L

L

L

L

T

D

Q

K

V

V

G

G

L

L

Q

K

D

D

K

K

A

A

N
x
H

A

A

Y
|
Y

P

P

S

D

S

S

L

L

S

S

G

G

G

G

Q

Q

K

A

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

M

M

E

E

P

P

D

K

L

I

M

M

L

L

F

F

D

D

E

E

P

P

T

T

S

S

A

A

L

L

D

D

P

P

E

E

M

M

V

V

G

G

D

E

V

V

L

L

D

S

V

V

M

M

K

K

D

Q

L

L

A

A

Q

N

K

E

G

G

M

M

T

T

M

M

V

V

I

V

V

V

T

T

H

H

E

E

M

M

G

G

F

F

A

A

R

R

D

E

V

V

S

G

D

D

R

R

V

V

I

L

F

F

M

M

D

D

G

G

G

G

Y

Y

V

I

V

I

E

E

S

E

N

G

I

K

P

P

E

E

E

D

L

L

F

F

T

D

R

R

P

P

K

Q

E

H

A

E

R

R

T

T

Q

K

S

A

F

F

L

L

S

S

K

K

V

V

L

F

binding adenosine-5'-diphosphate: F13 (≠ Y11), V18 (= V16), S38 (= S36), G39 (= G37), S40 (= S38), G41 (= G39), K42 (= K40), S43 (= S41), T44 (= T42), H133 (≠ N131), Y135 (= Y133)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
63% identity, 100% coverage: 1:240/241 of query aligns to 1:240/240 of 4ymuJ

query
sites
4ymuJ

M

M

I

I

N

F

I

V

T

N

N

D

L

V

H

Y

K

K

S

N

Y
x
F

G

G

D

S

N
x
L

A

E

V
|
V

L

L

K

K

G

G

I

V

N

T

E

L

H

K

I

V

R

N

Q

K

G

G

E

E

V

V

V

V

S

V

V

I

I

I

G

G

P

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

L

R

R

C

C

I

I

N

N

L

L

L

L

E
|
E

K

E

P

P

T

T

Q

K

G

G

D

E

I

V

E

F

I

I

E

D

G

G

Q

V

S

K

I

I

T

N

A

N

K

G

D

K

A

V

C

N

V

I

D

N

K

K

L

V

R

R

Q

Q

K

K

V

V

G

G

M

M

V

V

F

F

Q

Q

N

H

F

F

N

N

L

L

F

F

P

P

H

H

K

L

T

T

V

A

L

I

Q

E

N

N

I

I

T

T

L

L

A

A

P

P

V

V

S

K

L

V

K

K

L

K

M

M

T

N

Q

K

A

K

E

E

A

A

D

E

N

E

K

L

A

A

L

V

A

D

L

L

L

L

T

A

Q

K

V

V

G

G

L

L

Q

L

D

D

K

K

A

K

N

D

A

Q

Y

Y

P

P

S

I

S

K

L

L

S

S

G

G

G

G

Q

Q

K

K

Q

Q

R

R

V

L

A

A

I

I

A

A

R

R

A

A

L

L

A

A

M

M

E

Q

P

P

D

E

L

V

M

M

L

L

F

F

D

D

E
|
E

P

P

T

T

S

S

A

A

L

L

D

D

P

P

E

E

M

M

V

V

G

K

D

E

V

V

L

L

D

N

V

V

M

M

K

K

D

Q

L

L

A

A

Q

N

K

E

G

G

M

M

T

T

M

M

V

V

I

V

V

V

T

T

H
|
H

E

E

M

M

G

G

F

F

A

A

R

R

D

E

V

V

S

G

D

D

R

R

V

V

I

I

F

F

M

M

D

D

G

D

G

G

Y

V

V

I

V

V

E

E

S

E

N

G

I

T

P

P

E

E

E

E

L

I

F

F

T

Y

R

R

P

A

K

K

E

N

A

E

R

R

T

T

Q

R

S

E

F

F

L

L

S

S

K

K

V

I

L

L

binding adenosine-5'-triphosphate: F11 (≠ Y11), L14 (≠ N14), V16 (= V16), S36 (= S36), G37 (= G37), S38 (= S38), G39 (= G39), K40 (= K40), S41 (= S41), T42 (= T42), E51 (= E51), E162 (= E162), H194 (= H194)
binding magnesium ion: S41 (= S41), E162 (= E162)

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
63% identity, 100% coverage: 1:240/241 of query aligns to 2:240/241 of 4u00A

query
sites
4u00A

M

I

I

I

N

R

I

I

T

R

N

N

L

L

H

H

K

K

S

W

Y
x
F

G

G

D

P

N
x
L

A

H

V
|
V

L

L

K

K

G

G

I

I

N

H

E

L

H

E

I

V

R

A

Q

P

G

G

E

E

V

K

V

L

S

V

V

I

I

I

G

G

P

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

C

T

I

I

N

N

L

R

L

L

E

E

K

D

P

F

T

Q

Q

E

G

G

D

E

I

V

E

V

I

V

E

D

G

G

Q

L

S

S

I

V

T

K

A

-

K

D

D

D

A

R

C

A

V

L

D

R

K

E

L

I

R

R

Q

R

K

E

V

V

G

G

M

M

V

V

F

F

Q

Q

N

Q

F

F

N

N

L

L

F

F

P

P

H

H

K

M

T

T

V

V

L

L

Q

E

N

N

I

V

T

T

L

L

A

A

P

P

V

M

S

R

L

V

K

R

L

R

M

W

T

P

Q

R

A

E

E

K

A

A

D

E

N

K

K

K

A

A

L

L

A

E

L

L

L

L

T

E

Q

R

V

V

G

G

L

I

Q

L

D

D

K

Q

A

A

N

R

A

K

Y

Y

P

P

S

A

S

Q

L

L

S

S

G

G

G

G

Q

Q

K

Q

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

M

M

E

E

P

P

D

K

L

I

M

M

L

L

F

F

D

D

E

E

P

P

T

T

S

S

A

A

L

L

D

D

P

P

E

E

M

M

V

V

G

G

D

E

V

V

L

L

D

D

V

V

M

M

K

R

D

D

L

L

A

A

Q

Q

K

G

G

G

M

M

T

T

M

M

V

V

I

V

V

V

T

T

H

H

E

E

M

M

G

G

F

F

A

A

R

R

D

E

V

V

S

A

D

D

R

R

V

V

I

V

F

F

M

M

D

D

G

G

G

G

Y

Q

V

I

V

V

E

E

S

E

N

G

I

R

P

P

E

E

E

E

L

I

F

F

T

T

R

R

P

P

K

K

E

E

A

E

R

R

T

T

Q

R

S

S

F

F

L

L

S

Q

K

R

V

V

L

L

binding adenosine-5'-diphosphate: F12 (≠ Y11), L15 (≠ N14), V17 (= V16), S37 (= S36), G38 (= G37), S39 (= S38), G40 (= G39), K41 (= K40), S42 (= S41), T43 (= T42)

1b0uA Atp-binding subunit of the histidine permease from salmonella typhimurium (see paper)
52% identity, 100% coverage: 2:241/241 of query aligns to 3:254/258 of 1b0uA

query
sites
1b0uA

I

L

N

H

I

V

T

I

N

D

L

L

H

H

K

K

S

R

Y
|
Y

G

G

D

G

N
x
H

A

E

V
|
V

L

L

K

K

G

G

I

V

N

S

E

L

H

Q

I

A

R

R

Q

A

G

G

E

D

V

V

V

I

S

S

V

I

I

I

G

G

P

S

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

C

C

I

I

N

N

L

F

L

L

E

E

K

K

P

P

T

S

Q

E

G

G

D

A

I

I

E

I

I

V

E

N

G

G

Q

Q

S

N

I

I

T

N

A

L

-

V

-

R

-

D

K

K

D

D

A

G

-

Q

-

L

-

K

C

V

V

A

D

D

K

K

-

N

-

Q

-

L

-

R

-

L

L

L

R

R

Q

T

K

R

V

L

G

T

M

M

V

V

F

F

Q

Q

N

H

F

F

N

N

L

L

F

W

P

S

H

H

K

M

T

T

V

V

L

L

Q

E

N

N

I

V

T

M

L

E

A

A

P

P

V

I

S

Q

L

V

K

L

L

G

M

L

T

S

Q

K

A

H

E

D

A

A

D

R

N

E

K

R

A

A

L

L

A

K

L

Y

L

L

T

A

Q

K

V

V

G

G

L

I

Q

D

D

E

K

R

A

A

N

Q

A

G

-

K

Y

Y

P

P

S

V

S

H

L

L

S

S

G

G

G

G

Q

Q

K

Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

A

A

L

L

A

A

M

M

E

E

P

P

D

D

L

V

M

L

L

L

F

F

D

D

E

E

P

P

T

T

S

S

A

A

L

L

D

D

P

P

E

E

M

L

V

V

G

G

D

E

V

V

L

L

D

R

V

I

M

M

K

Q

D

Q

L

L

A

A

Q

E

K

E

G

G

M

K

T

T

M

M

V

V

I

V

V

V

T

T

H

H

E

E

M

M

G

G

F

F

A

A

R

R

D

H

V

V

S

S

D

S

R

H

V

V

I

I

F

F

M

L

D

H

G

Q

G

G

Y

K

V

I

V

E

E

E

S

E

N

G

I

D

P

P

E

E

E

Q

L

V

F

F

T

G

R

N

P

P

K

Q

E

S

A

P

R

R

T

L

Q

Q

S

Q

F

F

L

L

S

K

K

G

V

S

L

L

R

K

binding adenosine-5'-triphosphate: Y12 (= Y11), H15 (≠ N14), V17 (= V16), S37 (= S36), G38 (= G37), S39 (= S38), G40 (= G39), K41 (= K40), S42 (= S41), T43 (= T42)

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
40% identity, 100% coverage: 1:241/241 of query aligns to 1:246/343 of P30750

query
sites
P30750

M

M

I

I

N

K

I

L

T

S

N

N

L

I

H

T

K

K

S

V

Y

F

G

H

D

Q

N

G

A

T

-

R

-

T

-

I

-

Q

V

A

L

L

K

N

G

N

I

V

N

S

E

L

H

H

I

V

R

P

Q

A

G

G

E

Q

V

I

V

Y

S

G

V

V

I

I

G

G

P

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

C

C

I

V

N

N

L

L

L

L

E

E

K

R

P

P

T

T

Q

E

G

G

D

S

I

V

E

L

I

V

E

D

G

G

Q

Q

S

E

I

L

T

T

A

T

-

L

K

S

D

E

A

S

C

E

V

L

D

T

K

K

L

A

R

R

Q

R

K

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

N

H

F

F

N

N

L

L

F

L

P

S

H

S

K

R

T

T

V

V

L

F

Q

G

N

N

I

V

T

A

L

L

A

-

P

P

V

L

S

E

L

L

K

D

L

N

M

T

T

P

Q

K

A

D

E

E

A

V

D

K

N

R

K

R

A

V

L

T

A

E

L

L

L

L

T

S

Q

L

V

V

G

G

L

L

Q

G

D

D

K

K

A

H

N

D

A

S

Y

Y

P

P

S

S

S

N

L

L

S

S

G

G

G

G

Q

Q

K

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

M

S

E

N

P

P

D

K

L

V

M

L

L

L

F

C

D

D

E
|
E

P

A

T

T

S

S

A

A

L

L

D

D

P

P

E

A

M

T

V

T

G

R

D

S

V

I

L

L

D

E

V

L

M

L

K

K

D

D

L

I

A

N

Q

R

K

R

-

L

G

G

M

L

T

T

M

I

V

L

I

L

V

I

T

T

H

H

E

E

M

M

G

D

F

V

A

V

R

K

D

R

V

I

S

C

D

D

R

C

V

V

I

A

F

V

M

I

D

S

G

N

G

G

Y

E

V

L

V

I

E

E

S

Q

N

D

I

T

P

V

E

S

E

E

L

V

F

F

T

S

R

H

P

P

K

K

E

T

A

P

R

L

T

A

Q

Q

S

K

F

F

L

I

S

Q

K

S

V

T

L

L

R

H

40:46 (vs. 36:42, 86% identical) binding
E166 (= E162) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
40% identity, 100% coverage: 1:241/241 of query aligns to 2:247/344 of 6cvlD

query
sites
6cvlD

M

M

I

I

N

K

I

L

T

S

N

N

L

I

H

T

K

K

S

V

Y
x
F

G

H

D
x
Q

N

G

A

T

-

R

-

T

-
x
I

-

Q

V

A

L

L

K

N

G

N

I

V

N

S

E

L

H

H

I

V

R

P

Q

A

G

G

E

Q

V

I

V

Y

S

G

V

V

I

I

G

G

P

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

C

C

I

V

N

N

L

L

L

L

E

E

K

R

P

P

T

T

Q

E

G

G

D

S

I

V

E

L

I

V

E

D

G

G

Q

Q

S

E

I

L

T

T

A

T

-

L

K

S

D

E

A

S

C

E

V

L

D

T

K

K

L

A

R

R

Q

R

K

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

N

H

F

F

N

N

L

L

F

L

P

S

H

S

K

R

T

T

V

V

L

F

Q

G

N

N

I

V

T

A

L

L

A

-

P

P

V

L

S

E

L

L

K

D

L

N

M

T

T

P

Q

K

A

D

E

E

A

V

D

K

N

R

K

R

A

V

L

T

A

E

L

L

L

L

T

S

Q

L

V

V

G

G

L

L

Q

G

D

D

K

K

A

H

N

D

A

S

Y

Y

P

P

S

S

S
x
N

L
|
L

S
|
S

G
|
G

G
|
G

Q
|
Q

K

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

M

S

E

N

P

P

D

K

L

V

M

L

L

L

F

C

D

D

E

Q

P

A

T

T

S

S

A

A

L

L

D

D

P

P

E

A

M

T

V

T

G

R

D

S

V

I

L

L

D

E

V

L

M

L

K

K

D

D

L

I

A

N

Q

R

K

R

-

L

G

G

M

L

T

T

M

I

V

L

I

L

V

I

T

T

H
|
H

E

E

M

M

G

D

F

V

A

V

R

K

D

R

V

I

S

C

D

D

R

C

V

V

I

A

F

V

M

I

D

S

G

N

G

G

Y

E

V

L

V

I

E

E

S

Q

N

D

I

T

P

V

E

S

E

E

L

V

F

F

T

S

R

H

P

P

K

K

E

T

A

P

R

L

T

A

Q

Q

S

K

F

F

L

I

S

Q

K

S

V

T

L

L

R

H

binding phosphothiophosphoric acid-adenylate ester: F12 (≠ Y11), Q14 (≠ D13), I19 (vs. gap), S41 (= S36), G42 (= G37), A43 (≠ S38), G44 (= G39), K45 (= K40), S46 (= S41), T47 (= T42), N141 (≠ S136), L142 (= L137), S143 (= S138), G144 (= G139), G145 (= G140), Q146 (= Q141), H200 (= H194)

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
40% identity, 100% coverage: 1:241/241 of query aligns to 2:247/344 of 3tuzC

query
sites
3tuzC

M

M

I

I

N

K

I

L

T

S

N

N

L

I

H

T

K

K

S

V

Y
x
F

G

H

D
x
Q

N

G

A

T

-

R

-

T

-

I

-

Q

V
x
A

L

L

K

N

G

N

I

V

N

S

E

L

H

H

I

V

R

P

Q

A

G

G

E

Q

V

I

V

Y

S

G

V

V

I

I

G

G

P

A

S

S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

C

C

I

V

N

N

L

L

L

L

E

E

K

R

P

P

T

T

Q

E

G

G

D

S

I

V

E

L

I

V

E

D

G

G

Q

Q

S

E

I

L

T

T

A

T

-

L

K

S

D

E

A

S

C

E

V

L

D

T

K

K

L

A

R

R

Q

R

K

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

N

H

F

F

N

N

L

L

F

L

P

S

H

S

K

R

T

T

V

V

L

F

Q

G

N

N

I

V

T

A

L

L

A

-

P

P

V

L

S

E

L

L

K

D

L

N

M

T

T

P

Q

K

A

D

E

E

A

V

D

K

N

R

K

R

A

V

L

T

A

E

L

L

L

L

T

S

Q

L

V

V

G

G

L

L

Q

G

D

D

K

K

A

H

N

D

A

S

Y

Y

P

P

S

S

S

N

L

L

S

S

G

G

G

G

Q

Q

K

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

M

S

E

N

P

P

D

K

L

V

M

L

L

L

F

C

D

D

E

Q

P

A

T

T

S

S

A

A

L

L

D

D

P

P

E

A

M

T

V

T

G

R

D

S

V

I

L

L

D

E

V

L

M

L

K

K

D

D

L

I

A

N

Q

R

K

R

-

L

G

G

M

L

T

T

M

I

V

L

I

L

V

I

T

T

H

H

E

E

M

M

G

D

F

V

A

V

R

K

D

R

V

I

S

C

D

D

R

C

V

V

I

A

F

V

M

I

D

S

G

N

G

G

Y

E

V

L

V

I

E

E

S

Q

N

D

I

T

P

V

E

S

E

E

L

V

F

F

T

S

R

H

P

P

K

K

E

T

A

P

R

L

T

A

Q

Q

S

K

F

F

L

I

S

Q

K

S

V

T

L

L

R

H

binding adenosine-5'-diphosphate: F12 (≠ Y11), Q14 (≠ D13), A21 (≠ V16), G42 (= G37), A43 (≠ S38), G44 (= G39), K45 (= K40), S46 (= S41), T47 (= T42)

Sites not aligning to the query:

binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 312, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
40% identity, 100% coverage: 1:241/241 of query aligns to 2:247/344 of 3tuiC

query
sites
3tuiC

M

M

I

I

N

K

I

L

T

S

N

N

L

I

H

T

K

K

S

V

Y
x
F

G

H

D
x
Q

N

G

A

T

-

R

-

T

-

I

-

Q

V

A

L

L

K

N

G

N

I

V

N

S

E

L

H

H

I

V

R

P

Q

A

G

G

E

Q

V

I

V

Y

S

G

V

V

I

I

G

G

P

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

C

C

I

V

N

N

L

L

L

L

E

E

K

R

P

P

T

T

Q

E

G

G

D

S

I

V

E

L

I

V

E

D

G

G

Q

Q

S

E

I

L

T

T

A

T

-

L

K

S

D

E

A

S

C

E

V

L

D

T

K

K

L

A

R

R

Q

R

K

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

N

H

F

F

N

N

L

L

F

L

P

S

H

S

K

R

T

T

V

V

L

F

Q

G

N

N

I

V

T

A

L

L

A

-

P

P

V

L

S

E

L

L

K

D

L

N

M

T

T

P

Q

K

A

D

E

E

A

V

D

K

N

R

K

R

A

V

L

T

A

E

L

L

L

L

T

S

Q

L

V

V

G

G

L

L

Q

G

D

D

K

K

A

H

N

D

A

S

Y

Y

P

P

S

S

S

N

L

L

S

S

G

G

G

G

Q

Q

K

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

M

S

E

N

P

P

D

K

L

V

M

L

L

L

F

C

D

D

E

Q

P

A

T

T

S

S

A

A

L

L

D

D

P

P

E

A

M

T

V

T

G

R

D

S

V

I

L

L

D

E

V

L

M

L

K

K

D

D

L

I

A

N

Q

R

K

R

-

L

G

G

M

L

T

T

M

I

V

L

I

L

V

I

T

T

H

H

E

E

M

M

G

D

F

V

A

V

R

K

D

R

V

I

S

C

D

D

R

C

V

V

I

A

F

V

M

I

D

S

G

N

G

G

Y

E

V

L

V

I

E

E

S

Q

N

D

I

T

P

V

E

S

E

E

L

V

F

F

T

S

R

H

P

P

K

K

E

T

A

P

R

L

T

A

Q

Q

S

K

F

F

L

I

S

Q

K

S

V

T

L

L

R

H

binding adenosine-5'-diphosphate: F12 (≠ Y11), Q14 (≠ D13), S41 (= S36), G42 (= G37), A43 (≠ S38), G44 (= G39), K45 (= K40), S46 (= S41), T47 (= T42)

1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
43% identity, 89% coverage: 1:214/241 of query aligns to 1:223/232 of 1f3oA

query
sites
1f3oA

M

M

I

I

N

K

I

L

T

K

N

N

L

V

H

T

K

K

S

T

Y
|
Y

-

K

-

M

G

G

D

E

N

E

A

I

V

I

-

Y

-
x
A

L

L

K

K

G

N

I

V

N

N

E

L

H

N

I

I

R

K

Q

E

G

G

E

E

V

F

V

V

S

S

V

I

I

M

G

G

P

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

F

M

L

L

R

N

C

I

I

I

N

G

L

C

L

L

E

D

K

K

P

P

T

T

Q

E

G

G

D

E

I

V

E

Y

I

I

E

D

G

-

Q

-

S

N

I

I

T

K

A

T

K

N

D

D

A

L

C

D

V

D

D

D

K

E

L

L

-

T

-

K

-

I

-

R

R

R

Q

D

K

K

V

I

G

G

M

F

V

V

F

F

Q

Q

N

Q

F

F

N

N

L

L

F

I

P

P

H

L

K

L

T

T

V

A

L

L

Q

E

N

N

I

V

T

E

L

L

A

-

P

P

V

L

S

I

L

F

K

K

L

Y

-

R

-

G

-

A

M

M

T

S

Q

G

A

E

E

E

A

R

D

R

N

K

K

R

A

A

L

L

A

E

L

C

L

L

T

K

Q

M

V

A

G

E

L

L

Q

E

D

E

K

R

-

F

A

A

N

N

A

H

Y

K

P

P

S

N

S

Q

L

L

S

S

G

G

G

G

Q

Q

K

Q

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

M

N

E

N

P

P

D

P

L

I

M

I

L

L

F

A

D

D

E

E

P

P

T

T

S

G

A

A

L

L

D

D

P

S

E

K

M

T

V

G

G

E

D

K

V

I

L

M

D

Q

V

L

M

L

K

K

D

K

L

L

A

N

Q

E

K

E

-

D

G

G

M

K

T

T

M

V

V

V

I

V

V

V

T

T

H

H

E

D

M

I

G

N

F

V

A

A

R

R

D

-

V

F

S

G

D

E

R

R

V

I

I

I

F

Y

M

L

D

K

G

D

G

G

Y

E

V

V

binding adenosine-5'-diphosphate: Y11 (= Y11), A20 (vs. gap), S40 (= S36), G41 (= G37), S42 (= S38), G43 (= G39), K44 (= K40), S45 (= S41), T46 (= T42)

1l2tA Dimeric structure of mj0796, a bacterial abc transporter cassette (see paper)
43% identity, 89% coverage: 1:214/241 of query aligns to 1:223/230 of 1l2tA

query
sites
1l2tA

M

M

I

I

N

K

I

L

T

K

N

N

L

V

H

T

K

K

S

T

Y
|
Y

-

K

-
x
M

G

G

D

E

N

E

A

I

V
x
I

-

Y

-
x
A

L

L

K

K

G

N

I

V

N

N

E

L

H

N

I

I

R

K

Q

E

G

G

E

E

V

F

V

V

S

S

V

I

I

M

G

G

P
|
P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

F

M

L

L

R

N

C

I

I

I

N

G

L

C

L

L

E

D

K

K

P

P

T

T

Q

E

G

G

D

E

I

V

E

Y

I

I

E

D

G

-

Q

-

S

N

I

I

T

K

A

T

K

N

D

D

A

L

C

D

V

D

D

D

K

E

L

L

-

T

-

K

-

I

-

R

R

R

Q

D

K

K

V

I

G

G

M

F

V

V

F

F

Q

Q

N

Q

F

F

N

N

L

L

F

I

P

P

H

L

K

L

T

T

V

A

L

L

Q

E

N

N

I

V

T

E

L

L

A

-

P

P

V

L

S

I

L

F

K

K

L

Y

-

R

-

G

-

A

M

M

T

S

Q

G

A

E

E

E

A

R

D

R

N

K

K

R

A

A

L

L

A

E

L

C

L

L

T

K

Q

M

V

A

G

E

L

L

Q

E

D

E

K

R

-
x
F

A

A

N

N

A
x
H

Y

K

P

P

S

N

S
x
Q

L

L

S
|
S

G
|
G

G
|
G

Q
|
Q

K

Q

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

M

N

E

N

P

P

D

P

L

I

M

I

L

L

F

A

D

D

E

Q

P

P

T

T

S

G

A
|
A

L

L

D

D

P

S

E

K

M

T

V

G

G

E

D

K

V

I

L

M

D

Q

V

L

M

L

K

K

D

K

L

L

A

N

Q

E

K

E

-

D

G

G

M

K

T

T

M

V

V

V

I

V

V

V

T

T

H
|
H

E

D

M

I

G

N

F

V

A

A

R

R

D

-

V

F

S

G

D

E

R

R

V

I

I

I

F

Y

M

L

D

K

G

D

G

G

Y

E

V

V

binding adenosine-5'-triphosphate: Y11 (= Y11), M13 (vs. gap), I18 (≠ V16), A20 (vs. gap), P39 (= P35), S40 (= S36), G41 (= G37), S42 (= S38), G43 (= G39), K44 (= K40), S45 (= S41), T46 (= T42), F138 (vs. gap), H141 (≠ A132), Q145 (≠ S136), S147 (= S138), G148 (= G139), G149 (= G140), Q150 (= Q141), A175 (= A166), H204 (= H194)

P0AAH0 Phosphate import ATP-binding protein PstB; ABC phosphate transporter; Phosphate-transporting ATPase; EC 7.3.2.1 from Escherichia coli (strain K12) (see paper)
38% identity, 98% coverage: 2:237/241 of query aligns to 11:253/257 of P0AAH0

query
sites
P0AAH0

I

I

N

Q

I

V

T

R

N

N

L

L

H

N

K

F

S

Y

Y

Y

G

G

D

K

N

F

A

H

V

A

L

L

K

K

G

N

I

I

N

N

E

L

H

D

I

I

R

A

Q

K

G

N

E

Q

V

V

V

T

S

A

V

F

I

I

G

G

P

P

S

S

G

G

S

C

G
|
G

K
|
K

S

S

T

T

F

L

L

L

R

R

C

T

I

F

N

N

L

K

L

M

-

F

-

E

-

L

-

Y

-

P

E

E

K

Q

P

R

T

A

Q

E

G

G

D

E

I

I

E

L

I

L

E

D

G

G

Q

D

S

N

I

I

T

L

A

T

K

N

D

S

A

Q

C

D

V

I

D

A

K

L

L

L

R

R

Q

A

K

K

V

V

G

G

M

M

V

V

F

F

Q

Q

N

K

F

P

N

T

L

P

F

F

P

P

H

M

K

-

T

S

V

I

L

Y

Q

D

N

N

I

I

T

A

L

F

A

G

P

V

V

R

S

L

L

F

K

E

L

K

M

L

T

S

Q

R

A

A

E

D

A

M

D

D

N

E

K

R

A

V

L

Q

A

W

L

A

L

L

T

T

Q

K

V

A

G

A

L

L

-

W

-

N

-

E

-

T

Q

K

D

D

K

K

A

L

N

H

A

Q

Y

S

P

G

S

Y

S

S

L

L

S

S

G

G

G

G

Q

Q

K

Q

Q

Q

R

R

V

L

A

C

I

I

A

A

R

R

A

G

L

I

A

A

M

I

E

R

P

P

D

E

L

V

M

L

L

L

F

L

D

D

E

E

P

P

T

C

S

S

A

A

L

L

D

D

P

P

E

I

M

S

V

T

G

G

D

R

V

I

L

E

D

E

V

L

M

I

K

T

D

E

L

L

A

K

Q

Q

K

D

G

-

M

Y

T

T

M

V

V

V

I

I

V

V

T

T

H

H

E

N

M

M

G

Q

F

Q

A

A

R

A

D

R

V

C

S

S

D

D

R

H

V

T

I

A

F

F

M

M

D

Y

G

L

G

G

Y

E

V

L

V

I

E

E

S

F

N

S

I

N

P

T

E

D

E

D

L

L

F

F

T

T

R

K

P

P

K

A

E

K

A

K

R

Q

T

T

Q

E

S

D

F

Y

L

I

S

T

G48 (= G39) mutation to I: Loss of phosphate transport.
K49 (= K40) mutation to Q: Loss of phosphate transport.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

5xu1B Structure of a non-canonical abc transporter from streptococcus pneumoniae r6 (see paper)
40% identity, 88% coverage: 1:211/241 of query aligns to 3:218/226 of 5xu1B

query
sites
5xu1B

M

L

I

I

N

S

I

L

T

K

N

N

L

I

H

F

K

R

S

S

Y

Y

-

R

-

N

G

G

D

D

N

Q

A

E

-

L

-

Q

V

V

L

L

K

K

G

N

I

I

N

N

E

L

H

E

I

V

R

N

Q

E

G

G

E

E

V

F

V

V

S

A

V

I

I

M

G

G

P

P

S

S

G

G

S

S

G
|
G

K
|
K

S
|
S

T

T

F

L

L

M

R

N

C

T

I

I

N

G

L

M

L

L

E

D

K

T

P

P

T

T

Q

S

G

G

D

E

I

Y

E

Y

I

L

E

E

G

G

Q

Q

S

E

I

V

T

A

A

G

-

L

K

G

D

E

A

K

C

Q

V

L

D

A

K

K

L

V

R

R

-

N

Q

Q

K

Q

V

I

G

G

M

F

V

V

F

F

Q

Q

N

Q

F

F

N

F

L

L

F

L

P

S

H

K

K

L

T

N

V

A

L

L

Q

Q

N

N

I

V

T

E

L

L

A

-

P

P

V

L

S

I

L

Y

K

A

L

G

M

V

T

S

Q

S

A

S

E

K

A

R

D

R

N

K

K

L

A

A

L

E

A

E

L

Y

L

L

T

D

Q

K

V

V

G

E

L

L

Q

T

D

E

K

R

A

S

N

H

A

H

Y

L

P

P

S

S

S

E

L

L

S

S

G

G

G

G

Q

Q

K

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

V

M

N

E

N

P

P

D

S

L

I

M

I

L

L

F

A

D

D

E

E

P

P

T

T

S

G

A

A

L

L

D

D

P

T

E

K

M

T

V

G

G

N

D

Q

V

I

L

M

D

Q

V

L

M

L

K

V

D

D

L

L

A

N

Q

K

K

E

G

G

M

K

T

T

M

I

V

I

I

M

V

V

T

T

H

H

E

E

M

P

G

E

F

I

A

A

R

A

D

Y

V

A

S

K

D

R

R

Q

V

I

I

V

F

I

M

R

D

D

G

G

binding magnesium ion: G45 (= G39), K46 (= K40), S47 (= S41)

A5U7B7 Cell division ATP-binding protein FtsE from Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra) (see 2 papers)
40% identity, 90% coverage: 1:216/241 of query aligns to 1:218/229 of A5U7B7

query
sites
A5U7B7

M

M

I

I

N

T

I

L

T

D

N

H

L

V

H

T

K

K

S

Q

Y

Y

G

K

D

S

N

S

A

A

-

R

-

P

V

A

L

L

K

D

G

D

I

I

N

N

E

V

H

K

I

I

R

D

Q

K

G

G

E

E

V

F

V

V

S

F

V

L

I

I

G

G

P

P

S

S

G

G

S

S

G

G

K
|
K

S

S

T

T

F

F

L

M

R

R

C

L

I

L

N

L

L

A

L

A

E

E

K

T

P

P

T

T

Q

S

G

G

D

D

I

V

E

R

I

V

E

S

G

K

Q

F

S

H

I

V

T

N

A

K

-

L

K

R

D

G

A

R

C

H

V

V

D

P

K

K

L

L

R

R

Q

Q

K

V

V

I

G

G

M
x
C

V

V

F

F

Q

Q

N

D

F

F

N

R

L

L

F

L

P

Q

H

Q

K

K

T

T

V

V

L

Y

Q

D

N

N

I

V

T

A

L

F

A

A

P

-

V

-

S

-

L

L

K

E

L

V

M

I

-

G

T

K

Q

R

A

T

E

D

A

A

D

I

N

N

K

R

A

V

L

V

A

P

-

E

L

V

L

L

T

E

Q

T

V

V

G

G

L

L

Q

S

D

G

K

K

A

A

N

N

A

R

Y

L

P

P

S

D

S

E

L

L

S

S

G

G

G

G

Q

E

K

Q

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

F

A

V

M

N

E

R

P

P

D

L

L

V

M

L

L

L

F

A

D

D

E

E

P

P

T

T

S

G

A

N

L

L

D

D

P

P

E

E

M

T

V

S

G

R

D

D

V

I

L

M

D

D

V

L

M

L

K

E

D

R

L

I

A

N

Q

R

K

T

G

G

M

T

T

T

M

V

V

L

I

M

V

A

T

T

H

H

E

D

M

H

G

H

F

I

A

V

R

D

D

S

V

M

S

R

D

Q

R

R

V

V

I

V

F

E

M

L

D

S

G

L

G

G

Y

R

V

L

V

V

E

R

K42 (= K40) mutation to R: Does not bind ATP. Does not affect dimerization.
C84 (≠ M81) mutation to A: Does not affect ATPase activity.

2d62A Crystal structure of multiple sugar binding transport atp- binding protein
35% identity, 98% coverage: 2:236/241 of query aligns to 7:243/375 of 2d62A

query
sites
2d62A

I

V

N

K

I

L

T

I

N

N

L

I

H

W

K

K

S

R

Y

F

G

G

D

D

N

V

A

T

V

A

L

V

K

K

G

D

I

L

N

S

E

L

H

E

I

I

R

K

Q

D

G

G

E

E

V

F

V

L

S

V

V

L

I

L

G

G

P
|
P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
x
T

T
|
T

F

T

L

L

R

R

C

M

I

I

N

A

L

G

L

L

E

E

K

E

P

P

T

T

Q

R

G

G

D

Q

I

I

E

Y

I

I

E

E

G

D

Q

N

S

L

I

V

T

A

-

D

-

P

A

E

K

K

D

G

A

V

C

F

V

V

D

P

K

P

L

K

R

E

Q

R

K

D

V

V

G

A

M

M

V

V

F

F

Q

Q

N

S

F

Y

N

A

L

L

F

Y

P

P

H

H

K

M

T

T

V

V

L

Y

Q

D

N

N

I

I

T

A

L

F

A

-

P

P

V

L

S

K

L

L

K

R

L

K

M

V

T

P

Q

K

A

Q

E

E

A

I

D

D

N

K

K

R

A

V

L

R

A

E

L

V

L

A

T

E

Q

M

V

L

G

G

L

L

Q

T

D

E

K

L

A

L

N

N

A

R

Y

K

P

P

S

R

S

E

L

L

S

S

G

G

G

G

Q

Q

K

R

Q

Q

R

R

V

V

A

A

I

L

A

G

R

R

A

A

L

I

A

I

M

R

E

R

P

P

D

K

L

V

M

F

L

L

F

M

D

D

E

E

P

P

T

L

S

S

A

N

L

L

D

D

P

A

E

K

M

L

V

R

G

V

D

K

V

M

L

R

D

A

V

E

M

L

K

K

D

K

L

L

A

Q

-

R

Q

Q

K

L

G

G

M

V

T

T

M

T

V

I

I

Y

V

V

T

T

H

H

E

D

M

Q

G

V

F

E

A

A

R

M

D

T

V

M

S

G

D

D

R

R

V

I

I

A

F

V

M

M

D

N

G

K

G

G

Y

E

V

L

V

Q

E

Q

S

V

N

G

I

T

P

P

E

D

E

E

L

V

F

Y

T

Y

R

K

P

P

K

V

E

N

A

T

R

F

T

V

Q

A

S

G

F

F

L

I

binding pyrophosphate 2-: P40 (= P35), S41 (= S36), G42 (= G37), C43 (≠ S38), G44 (= G39), K45 (= K40), T46 (≠ S41), T47 (= T42)

7ahhC Opua inhibited inward-facing, sbd docked (see paper)
37% identity, 90% coverage: 21:236/241 of query aligns to 46:263/382 of 7ahhC

query
sites
7ahhC

N

N

E

F

H

E

I

I

R

N

Q

E

G

G

E

E

V

I

V

F

S

V

V

I

I

M

G

G

P

L

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

L

R

R

C

L

I

L

N

N

L

R

L

L

E

I

K

E

P

P

T

T

Q

S

G

G

D

K

I

I

E

F

I

I

E

D

G

N

Q

Q

S

D

I

V

T

A

A

T

-

L

-

N

K

K

D

E

A

D

C

L

V

L

D

Q

K

V

L

R

R

R

Q

K

K

T

V

M

G

S

M

M

V

V

F

F

Q

Q

N

N

F

F

N

G

L

L

F

F

P

P

H

H

K

R

T

T

V

I

L

L

Q

E

N

N

I

-

T

T

L

E

A

Y

P

G

V

L

S

E

L

V

K

Q

L

N

M

V

T

P

Q

K

A

E

E

E

A

R

D

R

N

K

K

R

A

A

L

E

A

K

L

A

L

L

T

D

Q

N

V

A

G

N

L

L

Q

L

D

D

K

F

A

K

N

D

A

Q

Y

Y

P

P

S

K

S

Q

L

L

S

S

G

G

G

G

Q

M

K

Q

Q

Q

R

R

V

V

A

G

I

L

A

A

R

R

A

A

L

L

A

A

M

N

E

D

P

P

D

E

L

I

M

L

L

L

F

M

D
|
D

E
|
E

P

A

T

F

S

S

A

A

L

L

D

D

P

P

E

L

M

I

V

R

G

R

D

E

V

M

L

Q

D

D

V

E

M

L

K

L

D

E

L

L

A

Q

Q

A

K

K

-

F

G

Q

M

K

T

T

M

I

V

I

I

F

V

V

T

S

H

H

E

D

M

L

G

N

F

E

A

A

R

L

D

R

V

I

S

G

D

D

R

R

V

I

I

A

F

I

M

M

D

K

G

D

G

G

Y

K

V

I

V

M

E

Q

S

I

N

G

I

T

P

G

E

E

E

E

L

I

F

L

T

T

R

N

P

P

K

A

E

N

A

D

R

Y

T

V

Q

K

S

T

F

F

L

V

binding phosphoaminophosphonic acid-adenylate ester: S61 (= S36), G62 (= G37), S63 (= S38), G64 (= G39), K65 (= K40), S66 (= S41), T67 (= T42), D187 (= D161), E188 (= E162)

Sites not aligning to the query:

7aheC Opua inhibited inward facing (see paper)
37% identity, 90% coverage: 21:236/241 of query aligns to 46:263/382 of 7aheC

query
sites
7aheC

N

N

E

F

H

E

I

I

R

N

Q

E

G

G

E

E

V

I

V

F

S

V

V

I

I

M

G

G

P

L

S

S

G

G

S

S

G

G

K

K

S

S

T

T

F

L

L

L

R

R

C

L

I

L

N

N

L

R

L

L

E

I

K

E

P

P

T

T

Q

S

G

G

D

K

I

I

E

F

I

I

E

D

G

N

Q

Q

S

D

I

V

T

A

A

T

-

L

-

N

K

K

D

E

A

D

C

L

V

L

D

Q

K

V

L

R

R

R

Q

K

K

T

V

M

G

S

M

M

V

V

F

F

Q

Q

N

N

F

F

N

G

L

L

F

F

P

P

H

H

K

R

T

T

V

I

L

L

Q

E

N

N

I

-

T

T

L

E

A

Y

P

G

V

L

S

E

L

V

K

Q

L

N

M

V

T

P

Q

K

A

E

E

E

A

R

D

R

N

K

K

R

A

A

L

E

A

K

L

A

L

L

T

D

Q

N

V

A

G

N

L

L

Q

L

D

D

K

F

A

K

N

D

A

Q

Y

Y

P

P

S

K

S

Q

L

L

S

S

G

G

G

G

Q

M

K

Q

Q

Q

R

R

V

V

A

G

I

L

A

A

R

R

A

A

L

L

A

A

M

N

E

D

P

P

D

E

L