SitesBLAST
Comparing 7024284 FitnessBrowser__ANA3:7024284 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5dt9A Crystal structure of a putative d-erythronate-4-phosphate dehydrogenase from vibrio cholerae
50% identity, 99% coverage: 1:372/376 of query aligns to 4:375/379 of 5dt9A
- active site: N95 (= N92), R212 (= R209), D236 (= D233), E241 (= E238), H258 (= H255)
- binding nicotinamide-adenine-dinucleotide: N95 (= N92), G129 (= G126), Q130 (≠ N127), V131 (≠ T128), D150 (= D147), K153 (= K150), H178 (= H175), T179 (≠ V176), P180 (= P177), W187 (≠ H184), T189 (= T186), A210 (≠ C207), A211 (≠ C208), H258 (= H255), A260 (= A257), G261 (= G258)
3oetH D-erythronate-4-phosphate dehydrogenase complexed with NAD
46% identity, 99% coverage: 1:374/376 of query aligns to 3:375/378 of 3oetH
- active site: N93 (= N92), R210 (= R209), D234 (= D233), E237 (= E236), E239 (= E238), G253 (≠ A252), H256 (= H255)
- binding nicotinamide-adenine-dinucleotide: N93 (= N92), V97 (= V96), G125 (= G124), G127 (= G126), N128 (= N127), V129 (≠ T128), D148 (= D147), P149 (= P148), P150 (≠ I149), H176 (= H175), T177 (≠ V176), P178 (= P177), Y185 (≠ H184), T187 (= T186), A208 (≠ C207), C209 (= C208), H256 (= H255), A258 (= A257)
2o4cA Crystal structure of d-erythronate-4-phosphate dehydrogenase complexed with NAD (see paper)
46% identity, 97% coverage: 1:363/376 of query aligns to 1:368/380 of 2o4cA
- active site: N91 (= N92), R208 (= R209), D232 (= D233), E237 (= E238), H254 (= H255)
- binding nicotinamide-adenine-dinucleotide: N91 (= N92), V95 (= V96), G123 (= G124), G125 (= G126), Q126 (≠ N127), V127 (≠ T128), D146 (= D147), P147 (= P148), P148 (≠ I149), H174 (= H175), T175 (≠ V176), P176 (= P177), H183 (= H184), T185 (= T186), A206 (≠ C207), S207 (≠ C208), H254 (= H255), A256 (= A257), G257 (= G258)
- binding phosphate ion: T66 (= T67), R346 (= R341)
Q9I3W9 Erythronate-4-phosphate dehydrogenase; EC 1.1.1.290 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
46% identity, 97% coverage: 1:363/376 of query aligns to 1:368/380 of Q9I3W9
- C65 (≠ A66) modified: Disulfide link with 90
- C90 (= C91) modified: Disulfide link with 65
- QV 126:127 (≠ NT 127:128) binding
- D146 (= D147) binding
- T175 (≠ V176) binding
- ASR 206:208 (≠ CCR 207:209) binding
- D232 (= D233) binding
- G257 (= G258) binding
1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
31% identity, 66% coverage: 33:279/376 of query aligns to 37:302/304 of 1wwkA
- active site: S96 (≠ N92), R230 (= R209), D254 (= D233), E259 (= E238), H278 (= H255)
- binding nicotinamide-adenine-dinucleotide: V100 (= V96), G146 (vs. gap), F147 (vs. gap), G148 (vs. gap), R149 (vs. gap), I150 (vs. gap), Y168 (≠ N146), D169 (= D147), P170 (= P148), V201 (= V176), P202 (= P177), T207 (= T186), T228 (≠ C207), S229 (≠ C208), D254 (= D233), H278 (= H255), G280 (≠ A257)
3kb6B Crystal structure of d-lactate dehydrogenase from aquifex aeolicus complexed with NAD and lactic acid (see paper)
28% identity, 68% coverage: 7:260/376 of query aligns to 11:299/334 of 3kb6B
- active site: S97 (≠ N92), R231 (= R209), D255 (= D233), E260 (= E238), H294 (= H255)
- binding lactic acid: F49 (≠ R44), S72 (≠ T67), V73 (≠ I68), G74 (= G69), Y96 (≠ C91), R231 (= R209), H294 (= H255)
- binding nicotinamide-adenine-dinucleotide: V73 (≠ I68), Y96 (≠ C91), V101 (= V96), G150 (= G126), R151 (≠ N127), I152 (≠ T128), D171 (= D147), V172 (≠ P148), P203 (= P177), T229 (≠ C207), A230 (≠ C208), R231 (= R209), H294 (= H255), A296 (= A257), Y297 (≠ G258)
P87228 Putative D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; EC 1.1.1.95; EC 1.1.1.399 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
30% identity, 55% coverage: 33:239/376 of query aligns to 93:314/466 of P87228
- S258 (≠ T179) modified: Phosphoserine
Sites not aligning to the query:
- 87 modified: Phosphoserine
7ewhA Crystal structure of human phgdh in complex with homoharringtonine (see paper)
28% identity, 62% coverage: 34:266/376 of query aligns to 39:289/302 of 7ewhA
- binding (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine: L146 (≠ V123), G147 (= G124), L148 (≠ A125), G149 (= G126), R150 (≠ N127), I151 (≠ T128), G152 (= G129), D170 (= D147), H201 (= H175), T202 (≠ V176), P203 (= P177)
6rihA Crystal structure of phgdh in complex with compound 9 (see paper)
28% identity, 62% coverage: 34:266/376 of query aligns to 39:289/302 of 6rihA
6plfA Crystal structure of human phgdh complexed with compound 1 (see paper)
28% identity, 62% coverage: 34:266/376 of query aligns to 40:290/305 of 6plfA
6cwaA Crystal structure phgdh in complex with nadh and 3-phosphoglycerate at 1.77 a resolution (see paper)
28% identity, 62% coverage: 34:266/376 of query aligns to 38:288/299 of 6cwaA
- binding 1,4-dihydronicotinamide adenine dinucleotide: N96 (= N92), A100 (≠ V96), R149 (≠ N127), I150 (≠ T128), Y168 (≠ N146), D169 (= D147), P170 (= P148), I171 (= I149), H200 (= H175), T201 (≠ V176), P202 (= P177), T207 (= T186), C228 (= C207), A229 (≠ C208), R230 (= R209), H277 (= H255), G279 (≠ A257)
6rj3A Crystal structure of phgdh in complex with compound 15 (see paper)
28% identity, 62% coverage: 34:266/376 of query aligns to 38:288/297 of 6rj3A
6plgA Crystal structure of human phgdh complexed with compound 15 (see paper)
28% identity, 62% coverage: 34:266/376 of query aligns to 39:289/303 of 6plgA
6rj5A Crystal structure of phgdh in complex with compound 39 (see paper)
28% identity, 62% coverage: 34:266/376 of query aligns to 39:289/301 of 6rj5A
7dkmA Phgdh covalently linked to oridonin (see paper)
28% identity, 62% coverage: 34:266/376 of query aligns to 40:290/306 of 7dkmA
- binding nicotinamide-adenine-dinucleotide: T74 (≠ I68), A102 (≠ V96), G148 (= G124), R151 (≠ N127), I152 (≠ T128), Y170 (≠ N146), D171 (= D147), P172 (= P148), I173 (= I149), H202 (= H175), T203 (≠ V176), P204 (= P177), T209 (= T186), C230 (= C207), A231 (≠ C208), R232 (= R209), H279 (= H255), G281 (≠ A257)
Sites not aligning to the query:
- binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: 14, 17, 18, 293
6rj2A Crystal structure of phgdh in complex with compound 40 (see paper)
28% identity, 62% coverage: 34:266/376 of query aligns to 36:286/299 of 6rj2A
- binding ~{N}-[(1~{R})-1-[4-(ethanoylsulfamoyl)phenyl]ethyl]-2-methyl-5-phenyl-pyrazole-3-carboxamide: G146 (= G126), I148 (≠ T128), Y166 (≠ N146), D167 (= D147), P168 (= P148), I169 (= I149), I170 (≠ K150), H198 (= H175), T199 (≠ V176), L208 (= L189), R228 (= R209)
O43175 D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; EC 1.1.1.95; EC 1.1.1.399; EC 1.1.1.37 from Homo sapiens (Human) (see 3 papers)
28% identity, 62% coverage: 34:266/376 of query aligns to 44:294/533 of O43175
- T78 (≠ I68) binding
- R135 (vs. gap) to W: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606949
- RI 155:156 (≠ NT 127:128) binding
- D175 (= D147) binding
- T207 (≠ V176) binding
- CAR 234:236 (≠ CCR 207:209) binding
- D260 (= D233) binding
- V261 (= V234) to M: in PHGDHD; results in a four-fold decrease in substrate affinity and a slight increase in maximal enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs267606947
- HLGA 283:286 (≠ HIAG 255:258) binding
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 373 A → T: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs201553627
- 377 G → S: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606948
- 425 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907988
- 490 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907987
2p9eA Crystal structure of g336v mutant of e.Coli phosphoglycerate dehydrogenase (see paper)
30% identity, 62% coverage: 33:264/376 of query aligns to 45:297/406 of 2p9eA
- active site: N104 (= N92), R236 (= R209), D260 (= D233), E265 (= E238), H288 (= H255)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G156 (= G126), H157 (≠ N127), I158 (≠ T128), Y176 (≠ N146), D177 (= D147), I178 (vs. gap), H206 (= H175), V207 (= V176), P208 (= P177), S212 (≠ T181), A234 (≠ C207), S235 (≠ C208), R236 (= R209), H288 (= H255), G290 (≠ A257)
4njmA Crystal structure of phosphoglycerate bound 3-phosphoglycerate dehydrogenase in entamoeba histolytica (see paper)
28% identity, 62% coverage: 1:234/376 of query aligns to 3:244/303 of 4njmA
Sites not aligning to the query:
4njoA Crystal structure of cofactor(NAD+) bound 3-phosphoglycerate dehydrogenase in entamoeba histolytica (see paper)
28% identity, 62% coverage: 1:234/376 of query aligns to 3:244/302 of 4njoA
- active site: N104 (= N92), R219 (= R209), D243 (= D233)
- binding nicotinamide-adenine-dinucleotide: A80 (≠ I68), V108 (= V96), G143 (= G126), Y144 (≠ N127), V145 (≠ T128), D164 (= D147), F166 (≠ I149), H189 (= H175), L190 (≠ V176), P191 (= P177), T217 (≠ C207), A218 (≠ C208), R219 (= R209)
Sites not aligning to the query:
Query Sequence
>7024284 FitnessBrowser__ANA3:7024284
MKIVVDENMPYVEPLFGALGEIIPVNGRTLTPEQVQDADVLLVRSVTRVNAALLDANSKL
KFVGSATIGTDHVDLAYLAGRGIPFSNAPGCNATAVGEFAFIAMLELAARFNSPLKGKVV
GIVGAGNTGSATAKCLEAYGIKVLLNDPIKAAEGDPRHFVSLETLLHEADIISLHVPITR
TGEHKTLHLFDEARMMSLKPNTWLLNCCRGDVIDNQALIKVKEQRDDLKLVLDVWEGEPN
PMPELVPFAEFATPHIAGYSLEGKARGTFMLYQKLCELLAIPATKRLSELLPPFHFKAVE
LEQAPDEKALLQLARFVYDLRDDDAVFRNGFARSNGFDTMRKNHKHRREFSALALAYHGQ
SEVDWLSNLGFSGVGR
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory