SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 7024383 Shewana3_1575 Na+/solute symporter (RefSeq) to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

P07117 Sodium/proline symporter; Proline carrier; Proline permease; Propionate transporter from Escherichia coli (strain K12) (see 4 papers)
29% identity, 20% coverage: 388:502/578 of query aligns to 325:439/502 of P07117

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P07117

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C344 (≠ G407) mutation to S: Small decrease in proline uptake activity. Confers resistance to N-ethylmaleimide. Exhibits low Na(+)-dependent proline binding.
C349 (≠ I412) mutation to S: Does not affect proline uptake activity. Sensitive to N-ethylmaleimide. Na(+)-dependent proline binding activity is similar to wild-type carrier.
R376 (= R439) mutation R->E,Q: No change in activity.; mutation to K: Loss of activity.

Sites not aligning to the query:

257 R→C: Sodium-independent binding affinity for proline.
281 C→S: Does not affect proline uptake activity. Confers resistance to N-ethylmaleimide. Na(+)-dependent proline binding activity is similar to wild-type carrier.

Q92911 Sodium/iodide cotransporter; Na(+)/I(-) cotransporter; Natrium iodide transporter; Sodium-iodide symporter; Na(+)/I(-) symporter; Solute carrier family 5 member 5 from Homo sapiens (Human) (see 3 papers)
23% identity, 38% coverage: 9:228/578 of query aligns to 24:233/643 of Q92911

query
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Q92911

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A102 (= A88) natural variant: A -> P
H226 (≠ N221) mutation H->A,D,E,K: Significant loss of iodide transport activity but no effect on its localization to the cell membrane.

Sites not aligning to the query:

237 D→A: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization.
242 Required for homodimerization; Y→A: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization. Reduced homodimerization; when associated with A-471. Loss of iodide transport activity; when associated with F-535.
243 Required for homodimerization; T→A: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization. Reduced homodimerization; when associated with A-471.
471 Required for homodimerization; Q→A: No effect on localization to the cell membrane, iodide transport activity and homodimerization. Significant loss of homodimerization; when associated with A-242 or A243.
525 A→F: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization. Loss of iodide transport activity; when associated with A-242.
536 T → Q: requires 2 nucleotide substitutions
556 S → Q: requires 2 nucleotide substitutions

Query Sequence

>7024383 Shewana3_1575 Na+/solute symporter (RefSeq)
MGVQGLTYLIVGLSFALYIGIAIWSRAGSTKEFYVAGGGVHPVVNGMATAADWMSAASFI
SLAGIVSFVGYDGSVYLMGWTGGYVLLALCMAPYLRKFGKFTVPDFVGERYYSQAARTVA
VICAIFICFTYIAGQMRGVGVVFSRFLEVDVDTGVYIGMAVVFFYAVLGGMKGITYTQVA
QYCVLIFAFMVPAIFLSVMMTGHIIPQIGFGAQLLDAAGNNSGVYLLEKLNNLSVDLGFA
PYTDGSKSMIDVFCITGALMVGTAGLPHVIVRFFTVPKVKDARVSAGWALVFIAIMYTTV
PALAAFSRVNMIETINGPDQKGVAYETAPDWIKNWEKTGLIKWDDKNGDGKIYYAKGKME
DAASPNEMKIDNDIIVLATPEIANLPAWVIALVAAGGLAAALSTSAGLLLVISTSVSHDL
LKKNLMPNISDKKELMYARLAAGIGIVIAGYFGVNPPGFVAAVVAFAFGLAASSLFPAII
MGIFSKTMNKEGAIAGMVIGLLFTAGYIIYFKFVDPTANVAANWFLGISPEGIGMVGMVV
NFAVAAIVCKVTAAAPAHVQDMVESIRFPKGAGEASSH

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory