SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 7024559 FitnessBrowser__ANA3:7024559 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7qh2C Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
27% identity, 45% coverage: 84:540/1013 of query aligns to 74:463/467 of 7qh2C

query
sites
7qh2C

R

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G

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x
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binding flavin-adenine dinucleotide: G75 (= G85), S76 (≠ G86), G77 (= G87), T78 (= T88), G79 (= G89), L80 (≠ T90), A83 (≠ Q93), C84 (≠ S94), P137 (≠ S147), G138 (≠ T148), E139 (≠ S149), A142 (= A152), T143 (= T153), G146 (= G156), N147 (≠ M157), S149 (≠ N159), T150 (= T160), A152 (= A162), G153 (≠ S163), E203 (= E267), G204 (= G268), I209 (= I273), E422 (= E500), H423 (= H501)
binding fe (iii) ion: H377 (= H456), H384 (= H463), E422 (= E500)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 73

8jdxA Crystal structure of mldhd in complex with 2-ketoisovaleric acid
22% identity, 52% coverage: 18:541/1013 of query aligns to 5:455/455 of 8jdxA

query
sites
8jdxA

Y

F

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Y

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binding flavin-adenine dinucleotide: E32 (≠ N45), P68 (≠ T80), G70 (≠ S82), T71 (≠ A83), G72 (≠ R84), T73 (≠ G85), G74 (= G86), G78 (≠ T90), V79 (≠ N91), L90 (≠ V103), P132 (≠ L146), G133 (≠ S147), A134 (≠ T148), G140 (= G156), M141 (= M157), A143 (≠ N159), T144 (= T160), A146 (= A162), S147 (= S163), E200 (= E267), G201 (= G268), I206 (= I273), W322 (vs. gap), E413 (= E500), H414 (= H501), N450 (= N536)
binding 3-methyl-2-oxobutanoic acid: R318 (= R403), H369 (= H456), H376 (= H463), H414 (= H501)
binding manganese (ii) ion: H369 (= H456), H376 (= H463), E413 (= E500)

8jdvA Crystal structure of mldhd in complex with 2-ketohexanoic acid
22% identity, 52% coverage: 18:541/1013 of query aligns to 5:454/454 of 8jdvA

query
sites
8jdvA

Y

F

L

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A

A

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C

-

R

-

E

-

K

-

R

-

A

-

L

-

I

-

E

-

Q

-

F

-

P

-

K

-

L

-

N

-

R

R

F

L

L

L

T

H

G

T

Y

A

D

G

L

R

K

G

N

R

V

H

W

Y

N

R

N

K

G

S

L

A

T

A

Q

G

F

Y

D

N

L

L

S

T

R

G

I

L

L

F

T

V

G

G

S

S

E
|
E

G
|
G

T

T

L

L

A

G

V

I

I
|
I

T

T

E

S

A

T

K

T

L

L

N

R

I

L

T

H

P

P

L

A

P

P

S

E

E

A

R

T

A

V

M

A

V

A

N

T

I

C

K

A

Y

F

D

P

S

S

F

V

Q

Q

S

A

A

A

L

V

R

D

H

S

A

T

P

V

S

Q

L

I

V

L

A

Q

A

A

R

A

A

V

T

P

V

V

-

A

-

R

V

I

E

E

T

F

V

L

D

D

S

D

K

V

V

M

L

M

N

D

L

A

A

C

R

N

E

R

D

-

I

-

V

-

W

-

H

H

S

S

V

-

S

-

D

-

L

-

I

-

Q

-

E

K

V

L

P

N

G

C

K

P

V

V

I

A

D

P

G

T

L

L

N

-

M

F

V

L

E

E

F

F

A

H

G

G

D

S

A

Q

V

Q

E

T

V

L

S

A

E

E

K

Q

L

L

A

Q

A

R

L

T

E

E

A

A

V

I

L

T

T

Q

E

D

Q

N

I

-

N

-

R

-

G

-

E

-

C

-

G

-

V

-

V

G

G

G

Y

S

Q

H

V

F

T

S

Q

A

D

E

K

K

A

R

S

N

I

-

E

-

K

E

I

L

Y

W

G

A

M

A

R
|
R

K

H

K

N

A

A

-
x
W

V
x
Y

G

A

L

A

L

L

G

A

A

L

T

S

K

P

G

G

R

S

R

K

K

-

P

-

I

-

A

A

F

Y

A

S

E

T

D

D

T

V

A

C

V

V

P

P

P

I

E

S

M

R

L

L

A

P

D

E

Y

I

I

L

M

V

E

E

F

T

R

K

A

E

L

E

L

I

D

K

S

A

H

S

N

K

L

L

Q

T

Y

G

G

A

M

I

F

V

G

G

H
|
H

V

V

D

G

A

D

G

G

V

N

L

F

H
|
H

V

C

R

I

P

L

A

L

L

V

D

D

M

P

C

D

D

D

V

A

E

E

D

E

E

Q

K

R

L

R

L

V

R

K

V

A

I

F

S

A

D

E

Q

N

V

L

A

G

A

R

L

R

T

A

L

L

K

A

Y

L

G

G

L

T

M

C

W

T

G

G

E
|
E

H
|
H

G

G

K

I

G

G

-

L

-

G

-

K

-

R

-

Q

-

L

V

L

R

Q

G

E

E

E

Y

V

G

G

P

P

S

-

V

-

F

V

G

G

D

V

E

E

L

-

Y

-

G

-

V

T

L

M

Q

R

D

Q

I

L

K

K

G

N

L

T

F

L

D

D

P

P

D

R

N

G

R

L

L

M

N
|
N

P

P

G

G

K

K

L

V

V

L

binding 2-Ketohexanoic acid: V75 (≠ G87), R317 (= R403), W321 (vs. gap), H368 (= H456), H375 (= H463), H413 (= H501)
binding flavin-adenine dinucleotide: P68 (≠ T80), G70 (≠ S82), T71 (≠ A83), G72 (≠ R84), T73 (≠ G85), G74 (= G86), G78 (≠ T90), V79 (≠ N91), L90 (≠ V103), P132 (≠ L146), G133 (≠ S147), A134 (≠ T148), G140 (= G156), M141 (= M157), A143 (≠ N159), T144 (= T160), A146 (= A162), S147 (= S163), E200 (= E267), G201 (= G268), I206 (= I273), W321 (vs. gap), Y322 (≠ V407), E412 (= E500), H413 (= H501), N449 (= N536)
binding manganese (ii) ion: H368 (= H456), H375 (= H463), E412 (= E500)

8jdsA Crystal structure of mldhd in complex with pyruvate
22% identity, 52% coverage: 18:541/1013 of query aligns to 5:456/456 of 8jdsA

query
sites
8jdsA

Y

F

L

V

D

E

A

A

L

L

A

K

Q

A

S

V

G

V

Y

G

A

S

G

P

D

H

I

V

D

S

K

T

R

A

Y

S

S

A

A

V

R

R

L

E

V

Q

Q

H

A

G

T

H

D

D

N
x
E

S

S

V

M

Y

H

Q

R

F

C

L

Q

P

P

-

P

Q

D

A

A

V

V

L

V

Y

W

P

P

K

Q

H

N

Q

V

K

D

D

Q

I

V

Q

S

I

R

A

V

L

A

S

S

L

L

A

C

A

Y

K

N

A

Q

E

G

F

V

A

P

G

I

V

I

T
x
P

F

F

S
x
G

A
x
T

R
x
G

G
x
T

G
|
G

G

V

T

E

G

G

T
x
G

N
x
V

G

C

Q

-

S

A

L

V

T

Q

H

G

G

G

L

V

I

C

L

I

D

N

V
x
L

S

T

R

-

Y

H

M

M

N

D

R

Q

V

I

L

T

E

E

V

L

N

N

H

T

E

E

Q

D

G

F

W

S

V

V

R

V

V

V

E

E

A

P

G

G

V

V

V

T

K

R

D

K

A

A

L

L

N

N

D

T

A

H

L

L

R

R

P

D

H

S

G

G

Y

L

F

W

F

F

S

P

P

V

D

D

L
x
P

S
x
G

T
x
A

S

D

N

-

R

-

A

A

T

S

I

L

G

C

G
|
G

M
|
M

I

A

N
x
A

T
|
T

D

G

A
|
A

S
|
S

G

G

A

T

G

N

S

A

L

V

V

R

Y

Y

G

G

K

T

T

M

S

R

D

D

H

N

V

V

L

I

A

N

L

L

R

E

S

V

I

V

L

L

I

-

D

-

G

-

S

-

V

-

F

-

D

-

T

-

R

-

P

-

L

-

D

-

A

-

G

-

L

-

C

-

N

-

P

P

D

D

N

G

V

-

S

-

D

-

N

-

P

-

L

-

G

-

Q

-

K

-

L

-

I

-

S

-

A

-

I

-

A

-

E

-

V

-

C

-

R

-

E

-

K

-

R

-

A

-

L

-

I

-

E

-

Q

-

F

-

P

-

K

-

L

-

N

-

R

R

F

L

L

L

T

H

G

T

Y

A

D

G

L

R

K

G

N

R

V

H

W

Y

N

R

N

K

G

S

L

A

T

A

Q

G

F

Y

D

N

L

L

S

T

R

G

I

L

L

F

T

V

G

G

S

S

E
|
E

G
|
G

T

T

L

L

A

G

V

I

I
|
I

T

T

E

S

A

T

K

T

L

L

N

R

I

L

T

H

P

P

L

A

P

P

S

E

E

A

R

T

A

V

M

A

V

A

N

T

I

C

K

A

Y

F

D

P

S

S

F

V

Q

Q

S

A

A

A

L

V

R

D

H

S

A

T

P

V

S

Q

L

I

V

L

A

Q

A

A

R

A

A

V

T

P

V

V

-

A

-

R

V

I

E

E

T

F

V

L

D

D

S

D

K

V

V

M

L

M

N

D

L

A

A

C

R

N

E

R

D

-

I

-

V

-

W

-

H

H

S

S

V

-

S

-

D

-

L

-

I

-

Q

-

E

K

V

L

P

N

G

C

K

P

V

V

I

A

D

P

G

T

L

L

N

-

M

F

V

L

E

E

F

F

A

H

G

G

D

S

A

Q

V

Q

E

T

V

L

S

A

E

E

K

Q

L

L

A

Q

A

R

L

T

E

E

A

A

V

I

L

T

T

Q

E

D

Q

N

I

-

N

-

R

-

G

-

E

-

C

-

G

-

V

-

V

G

G

G

Y

S

Q

H

V

F

T

S

Q

W

D

A

K

A

A

E

S

K

I

R

E

N

K

E

I

L

Y

W

G

A

M

A

R
|
R

K

H

K

N

A

A

-
x
W

V

Y

G

A

L

A

L

L

G

A

A

L

T

S

K

P

G

G

R

S

R

K

K

-

P

-

I

-

A

A

F

Y

A

S

E

T

D

D

T

V

A

C

V

V

P

P

P

I

E

S

M

R

L

L

A

P

D

E

Y

I

I

L

M

V

E

E

F

T

R

K

A

E

L

E

L

I

D

K

S

A

H

S

N

K

L

L

Q

T

Y

G

G

A

M

I

F

V

G

G

H
|
H

V

V

D

G

A

D

G

G

V

N

L

F

H
|
H

V

C

R

I

P

L

A

L

L

V

D

D

M

P

C

D

D

D

V

A

E

E

D

E

E

Q

K

R

L

R

L

V

R

K

V

A

I

F

S

A

D

E

Q

N

V

L

A

G

A

R

L

R

T

A

L

L

K

A

Y

L

G

G

L

T

M

C

W

T

G

G

E
|
E

H
|
H

G

G

K

I

G

G

-

L

-

G

-

K

-

R

-

Q

-

L

V

L

R

Q

G

E

E

E

Y

V

G

G

P

P

S

-

V

-

F

V

G

G

D

V

E

E

L

-

Y

-

G

-

V

T

L

M

Q

R

D

Q

I

L

K

K

G

N

L

T

F

L

D

D

P

P

D

R

N

G

R

L

L

M

N
|
N

P

P

G

G

K

K

L

V

V

L

binding flavin-adenine dinucleotide: E32 (≠ N45), P68 (≠ T80), G70 (≠ S82), T71 (≠ A83), G72 (≠ R84), T73 (≠ G85), G74 (= G86), G78 (≠ T90), V79 (≠ N91), L90 (≠ V103), P132 (≠ L146), G133 (≠ S147), A134 (≠ T148), G140 (= G156), M141 (= M157), A143 (≠ N159), T144 (= T160), A146 (= A162), S147 (= S163), E200 (= E267), G201 (= G268), I206 (= I273), W323 (vs. gap), E414 (= E500), H415 (= H501), N451 (= N536)
binding manganese (ii) ion: H370 (= H456), H377 (= H463), E414 (= E500)
binding pyruvic acid: R319 (= R403), H370 (= H456), H377 (= H463), H415 (= H501)

8jdeA Crystal structure of mldhd in complex with d-lactate
22% identity, 52% coverage: 18:541/1013 of query aligns to 5:455/455 of 8jdeA

query
sites
8jdeA

Y

F

L

V

D

E

A

A

L

L

A

K

Q

A

S

V

G

V

Y

G

A

S

G

P

D

H

I

V

D

S

K

T

R

A

Y

S

S

A

A

V

R

R

L

E

V

Q

Q

H

A

G

T

H

D

D

N

E

S

S

V

M

Y

H

Q

R

F

C

L

Q

P

P

-

P

Q

D

A

A

V

V

L

V

Y

W

P

P

K

Q

H

N

Q

V

K

D

D

Q

I

V

Q

S

I

R

A

V

L

A

S

S

L

L

A

C

A

Y

K

N

A

Q

E

G

F

V

A

P

G

I

V

I

T
x
P

F

F

S
x
G

A
x
T

R
x
G

G
x
T

G
|
G

G

V

T

E

G

G

T
x
G

N
x
V

G

C

Q

-

S

A

L

V

T

Q

H

G

G

G

L

V

I

C

L

I

D

N

V
x
L

S

T

R

-

Y

H

M

M

N

D

R

Q

V

I

L

T

E

E

V

L

N

N

H

T

E

E

Q

D

G

F

W

S

V

V

R

V

V

V

E

E

A

P

G

G

V

V

V

T

K

R

D

K

A

A

L

L

N

N

D

T

A

H

L

L

R

R

P

D

H

S

G

G

Y

L

F

W

F

F

S

P

P

V

D

D

L
x
P

S
x
G

T
x
A

S

D

N

-

R

-

A

A

T

S

I

L

G

C

G
|
G

M
|
M

I

A

N
x
A

T
|
T

D

G

A
|
A

S
|
S

G

G

A

T

G

N

S

A

L

V

V

R

Y

Y

G

G

K

T

T

M

S

R

D

D

H

N

V

V

L

I

A

N

L

L

R

E

S

V

I

V

L

L

I

-

D

-

G

-

S

-

V

-

F

-

D

-

T

-

R

-

P

-

L

-

D

-

A

-

G

-

L

-

C

-

N

-

P

P

D

D

N

G

V

-

S

-

D

-

N

-

P

-

L

-

G

-

Q

-

K

-

L

-

I

-

S

-

A

-

I

-

A

-

E

-

V

-

C

-

R

-

E

-

K

-

R

-

A

-

L

-

I

-

E

-

Q

-

F

-

P

-

K

-

L

-

N

-

R

R

F

L

L

L

T

H

G

T

Y

A

D

G

L

R

K

G

N

R

V

H

W

Y

N

R

N

K

G

S

L

A

T

A

Q

G

F

Y

D

N

L

L

S

T

R

G

I

L

L

F

T

V

G

G

S

S

E
|
E

G
|
G

T

T

L

L

A

G

V

I

I
|
I

T

T

E

S

A

T

K

T

L

L

N

R

I

L

T

H

P

P

L

A

P

P

S

E

E

A

R

T

A

V

M

A

V

A

N

T

I

C

K

A

Y

F

D

P

S

S

F

V

Q

Q

S

A

A

A

L

V

R

D

H

S

A

T

P

V

S

Q

L

I

V

L

A

Q

A

A

R

A

A

V

T

P

V

V

-

A

-

R

V

I

E

E

T

F

V

L

D

D

S

D

K

V

V

M

L

M

N

D

L

A

A

C

R

N

E

R

D

-

I

-

V

-

W

-

H

H

S

S

V

-

S

-

D

-

L

-

I

-

Q

-

E

K

V

L

P

N

G

C

K

P

V

V

I

A

D

P

G

T

L

L

N

-

M

F

V

L

E

E

F

F

A

H

G

G

D

S

A

Q

V

Q

E

T

V

L

S

A

E

E

K

Q

L

L

A

Q

A

R

L

T

E

E

A

A

V

I

L

T

T

Q

E

D

Q

N

I

-

N

-

R

-

G

-

E

-

C

-

G

G

V

G

V

S

G

H

Y

F

Q

S

V

W

T

A

Q

A

D

E

K

K

A

-

S

-

I

-

E

N

K

E

I

L

Y

W

G

A

M

A

R

R

K

H

K

N

A

A

-
x
W

V

Y

G

A

L

A

L

L

G

A

A

L

T

S

K

P

G

G

R

S

R

K

K

-

P

-

I

-

A

A

F

Y

A

S

E

T

D

D

T

V

A

C

V

V

P

P

P

I

E

S

M

R

L

L

A

P

D

E

Y

I

I

L

M

V

E

E

F

T

R

K

A

E

L

E

L

I

D

K

S

A

H

S

N

K

L

L

Q

T

Y

G

G

A

M

I

F

V

G

G

H
|
H

V

V

D

G

A

D

G

G

V

N

L

F

H
|
H

V

C

R

I

P

L

A

L

L

V

D

D

M

P

C

D

D

D

V

A

E

E

D

E

E

Q

K

R

L

R

L

V

R

K

V

A

I

F

S

A

D

E

Q

N

V

L

A

G

A

R

L

R

T

A

L

L

K

A

Y

L

G

G

L

T

M

C

W

T

G

G

E
|
E

H
|
H

G

G

K

I

G

G

-

L

-

G

-

K

-

R

-

Q

-

L

V

L

R

Q

G

E

E

E

Y

V

G

G

P

P

S

-

V

-

F

V

G

G

D

V

E

E

L

-

Y

-

G

-

V

T

L

M

Q

R

D

Q

I

L

K

K

G

N

L

T

F

L

D

D

P

P

D

R

N

G

R

L

L

M

N
|
N

P

P

G

G

K

K

L

V

V

L

binding flavin-adenine dinucleotide: P68 (≠ T80), G70 (≠ S82), T71 (≠ A83), G72 (≠ R84), T73 (≠ G85), G74 (= G86), G78 (≠ T90), V79 (≠ N91), L90 (≠ V103), P132 (≠ L146), G133 (≠ S147), A134 (≠ T148), G140 (= G156), M141 (= M157), A143 (≠ N159), T144 (= T160), A146 (= A162), S147 (= S163), E200 (= E267), G201 (= G268), I206 (= I273), W322 (vs. gap), E413 (= E500), H414 (= H501), N450 (= N536)
binding manganese (ii) ion: H369 (= H456), H376 (= H463), E413 (= E500)

8jduA Crystal structure of mldhd in complex with 2-ketovaleric acid
22% identity, 52% coverage: 18:541/1013 of query aligns to 5:455/455 of 8jduA

query
sites
8jduA

Y

F

L

V

D

E

A

A

L

L

A

K

Q

A

S

V

G

V

Y

G

A

S

G

P

D

H

I

V

D

S

K

T

R

A

Y

S

S

A

A

V

R

R

L

E

V

Q

Q

H

A

G

T

H

D

D

N

E

S

S

V

M

Y

H

Q

R

F

C

L

Q

P

P

-

P

Q

D

A

A

V

V

L

V

Y

W

P

P

K

Q

H

N

Q

V

K

D

D

Q

I

V

Q

S

I

R

A

V

L

A

S

S

L

L

A

C

A

Y

K

N

A

Q

E

G

F

V

A

P

G

I

V

I

T
x
P

F

F

S
x
G

A
x
T

R
x
G

G
x
T

G
|
G

G

V

T

E

G

G

T
x
G

N
x
V

G

C

Q

-

S

A

L

V

T

Q

H

G

G

G

L

V

I

C

L

I

D

N

V
x
L

S

T

R

-

Y

H

M

M

N

D

R

Q

V

I

L

T

E

E

V

L

N

N

H

T

E

E

Q

D

G

F

W

S

V

V

R

V

V

V

E

E

A

P

G

G

V

V

V

T

K

R

D

K

A

A

L

L

N

N

D

T

A

H

L

L

R

R

P

D

H

S

G

G

Y

L

F

W

F

F

S

P

P

V

D

D

L
x
P

S
x
G

T
x
A

S

D

N

-

R

-

A

A

T

S

I

L

G

C

G
|
G

M
|
M

I

A

N
x
A

T
|
T

D

G

A
|
A

S
|
S

G

G

A

T

G

N

S

A

L

V

V

R

Y

Y

G

G

K

T

T

M

S

R

D

D

H

N

V

V

L

I

A

N

L

L

R

E

S

V

I

V

L

L

I

-

D

-

G

-

S

-

V

-

F

-

D

-

T

-

R

-

P

-

L

-

D

-

A

-

G

-

L

-

C

-

N

-

P

P

D

D

N

G

V

-

S

-

D

-

N

-

P

-

L

-

G

-

Q

-

K

-

L

-

I

-

S

-

A

-

I

-

A

-

E

-

V

-

C

-

R

-

E

-

K

-

R

-

A

-

L

-

I

-

E

-

Q

-

F

-

P

-

K

-

L

-

N

-

R

R

F

L

L

L

T

H

G

T

Y

A

D

G

L

R

K

G

N

R

V

H

W

Y

N

R

N

K

G

S

L

A

T

A

Q

G

F

Y

D

N

L

L

S

T

R

G

I

L

L

F

T

V

G

G

S

S

E
|
E

G
|
G

T

T

L

L

A

G

V

I

I
|
I

T

T

E

S

A

T

K

T

L

L

N

R

I

L

T

H

P

P

L

A

P

P

S

E

E

A

R

T

A

V

M

A

V

A

N

T

I

C

K

A

Y

F

D

P

S

S

F

V

Q

Q

S

A

A

A

L

V

R

D

H

S

A

T

P

V

S

Q

L

I

V

L

A

Q

A

A

R

A

A

V

T

P

V

V

-

A

-

R

V

I

E

E

T

F

V

L

D

D

S

D

K

V

V

M

L

M

N

D

L

A

A

C

R

N

E

R

D

-

I

-

V

-

W

-

H

H

S

S

V

-

S

-

D

-

L

-

I

-

Q

-

E

K

V

L

P

N

G

C

K

P

V

V

I

A

D

P

G

T

L

L

N

-

M

F

V

L

E

E

F

F

A

H

G

G

D

S

A

Q

V

Q

E

T

V

L

S

A

E

E

K

Q

L

L

A

Q

A

R

L

T

E

E

A

A

V

I

L

T

T

Q

E

N

Q

G

I

-

N

-

R

-

G

-

E

-

C

-

G

-

V

-

G

G

Y

S

Q

H

V

F

T

S

Q

W

D

A

K

A

A

E

S

K

I

R

E

N

K

E

I

L

Y

W

G

A

M

A

R
|
R

K

H

K

N

A

A

-
x
W

V

Y

G

A

L

A

L

L

G

A

A

L

T

S

K

P

G

G

R

S

R

K

K

-

P

-

I

-

A

A

F

Y

A

S

E

T

D

D

T

V

A

C

V

V

P

P

P

I

E

S

M

R

L

L

A

P

D

E

Y

I

I

L

M

V

E

E

F

T

R

K

A

E

L

E

L

I

D

K

S

A

H

S

N

K

L

L

Q

T

Y

G

G

A

M

I

F

V

G

G

H
|
H

V

V

D

G

A

D

G

G

V

N

L

F

H
|
H

V

C

R

I

P

L

A

L

L

V

D

D

M

P

C

D

D

D

V

A

E

E

D

E

E

Q

K

R

L

R

L

V

R

K

V

A

I

F

S

A

D

E

Q

N

V

L

A

G

A

R

L

R

T

A

L

L

K

A

Y

L

G

G

L

T

M

C

W

T

G

G

E
|
E

H
|
H

G

G

K

I

G

G

-

L

-

G

-

K

-

R

-

Q

-

L

V

L

R

Q

G

E

E

E

Y

V

G

G

P

P

S

-

V

-

F

V

G

G

D

V

E

E

L

-

Y

-

G

-

V

T

L

M

Q

R

D

Q

I

L

K

K

G

N

L

T

F

L

D

D

P

P

D

R

N

G

R

L

L

M

N
|
N

P

P

G

G

K

K

L

V

V

L

binding 2-oxopentanoic acid: R318 (= R403), W322 (vs. gap), H369 (= H456), H376 (= H463), H414 (= H501)
binding flavin-adenine dinucleotide: P68 (≠ T80), G70 (≠ S82), T71 (≠ A83), G72 (≠ R84), T73 (≠ G85), G74 (= G86), G78 (≠ T90), V79 (≠ N91), L90 (≠ V103), P132 (≠ L146), G133 (≠ S147), A134 (≠ T148), G140 (= G156), M141 (= M157), A143 (≠ N159), T144 (= T160), A146 (= A162), S147 (= S163), E200 (= E267), G201 (= G268), I206 (= I273), W322 (vs. gap), E413 (= E500), N450 (= N536)
binding manganese (ii) ion: H369 (= H456), H376 (= H463), E413 (= E500)

8jdtA Crystal structure of mldhd in complex with 2-ketobutanoic acid
22% identity, 52% coverage: 18:541/1013 of query aligns to 5:455/455 of 8jdtA

query
sites
8jdtA

Y

F

L

V

D

E

A

A

L

L

A

K

Q

A

S

V

G

V

Y

G

A

S

G

P

D

H

I

V

D

S

K

T

R

A

Y

S

S

A

A

V

R

R

L

E

V

Q

Q

H

A

G

T

H

D

D

N

E

S

S

V

M

Y

H

Q

R

F

C

L

Q

P

P

-

P

Q

D

A

A

V

V

L

V

Y

W

P

P

K

Q

H

N

Q

V

K

D

D

Q

I

V

Q

S

I

R

A

V

L

A

S

S

L

L

A

C

A

Y

K

N

A

Q

E

G

F

V

A

P

G

I

V

I

T
x
P

F

F

S
x
G

A
x
T

R
x
G

G
x
T

G
|
G

G

V

T

E

G

G

T
x
G

N
x
V

G

C

Q

-

S

A

L

V

T

Q

H

G

G

G

L

V

I

C

L

I

D

N

V
x
L

S

T

R

-

Y

H

M

M

N

D

R

Q

V

I

L

T

E

E

V

L

N

N

H

T

E

E

Q

D

G

F

W

S

V

V

R

V

V

V

E

E

A

P

G

G

V

V

V

T

K

R

D

K

A

A

L

L

N

N

D

T

A

H

L

L

R

R

P

D

H

S

G

G

Y

L

F

W

F

F

S

P

P

V

D

D

L
x
P

S
x
G

T
x
A

S

D

N

-

R

-

A

A

T

S

I

L

G

C

G
|
G

M
|
M

I

A

N
x
A

T
|
T

D

G

A
|
A

S
|
S

G

G

A

T

G

N

S

A

L

V

V

R

Y

Y

G

G

K

T

T

M

S

R

D

D

H

N

V

V

L

I

A

N

L

L

R

E

S

V

I

V

L

L

I

-

D

-

G

-

S

-

V

-

F

-

D

-

T

-

R

-

P

-

L

-

D

-

A

-

G

-

L

-

C

-

N

-

P

P

D

D

N

G

V

-

S

-

D

-

N

-

P

-

L

-

G

-

Q

-

K

-

L

-

I

-

S

-

A

-

I

-

A

-

E

-

V

-

C

-

R

-

E

-

K

-

R

-

A

-

L

-

I

-

E

-

Q

-

F

-

P

-

K

-

L

-

N

-

R

R

F

L

L

L

T

H

G

T

Y

A

D

G

L

R

K

G

N

R

V

H

W

Y

N

R

N

K

G

S

L

A

T

A

Q

G

F

Y

D

N

L

L

S

T

R

G

I

L

L

F

T

V

G

G

S

S

E
|
E

G
|
G

T

T

L

L

A

G

V

I

I
|
I

T

T

E

S

A

T

K

T

L

L

N

R

I

L

T

H

P

P

L

A

P

P

S

E

E

A

R

T

A

V

M

A

V

A

N

T

I

C

K

A

Y

F

D

P

S

S

F

V

Q

Q

S

A

A

A

L

V

R

D

H

S

A

T

P

V

S

Q

L

I

V

L

A

Q

A

A

R

A

A

V

T

P

V

V

-

A

-

R

V

I

E

E

T

F

V

L

D

D

S

D

K

V

V

M

L

M

N

D

L

A

A

C

R

N

E

R

D

-

I

-

V

-

W

-

H

H

S

S

V

-

S

-

D

-

L

-

I

-

Q

-

E

K

V

L

P

N

G

C

K

P

V

V

I

A

D

P

G

T

L

L

N

-

M

F

V

L

E

E

F

F

A

H

G

G

D

S

A

Q

V

Q

E

T

V

L

S

A

E

E

K

Q

L

L

A

Q

A

R

L

T

E

E

A

A

V

I

L

T

T

Q

E

D

Q

N

I

-

N

-

R

-

G

-

E

-

C

-

G

G

V

G

V

S

G

H

Y

F

Q

S

V

W

T

A

Q

E

D

K

K

R

A

-

S

-

I

-

E

N

K

E

I

L

Y

W

G

A

M

A

R
|
R

K

H

K

N

A

A

-
x
W

V

Y

G

A

L

A

L

L

G

A

A

L

T

S

K

P

G

G

R

S

R

K

K

-

P

-

I

-

A

A

F

Y

A

S

E

T

D

D

T

V

A

C

V

V

P

P

P

I

E

S

M

R

L

L

A

P

D

E

Y

I

I

L

M

V

E

E

F

T

R

K

A

E

L

E

L

I

D

K

S

A

H

S

N

K

L

L

Q

T

Y

G

G

A

M

I

F

V

G

G

H
|
H

V

V

D

G

A

D

G

G

V

N

L

F

H
|
H

V

C

R

I

P

L

A

L

L

V

D

D

M

P

C

D

D

D

V

A

E

E

D

E

E

Q

K

R

L

R

L

V

R

K

V

A

I

F

S

A

D

E

Q

N

V

L

A

G

A

R

L

R

T

A

L

L

K

A

Y

L

G

G

L

T

M

C

W

T

G

G

E
|
E

H
|
H

G

G

K

I

G

G

-

L

-

G

-

K

-

R

-

Q

-

L

V

L

R

Q

G

E

E

E

Y

V

G

G

P

P

S

-

V

-

F

V

G

G

D

V

E

E

L

-

Y

-

G

-

V

T

L

M

Q

R

D

Q

I

L

K

K

G

N

L

T

F

L

D

D

P

P

D

R

N

G

R

L

L

M

N
|
N

P

P

G

G

K

K

L

V

V

L

binding 2-ketobutyric acid: R318 (= R403), H369 (= H456), H376 (= H463), H414 (= H501)
binding flavin-adenine dinucleotide: P68 (≠ T80), G70 (≠ S82), T71 (≠ A83), G72 (≠ R84), T73 (≠ G85), G74 (= G86), G78 (≠ T90), V79 (≠ N91), L90 (≠ V103), P132 (≠ L146), G133 (≠ S147), A134 (≠ T148), G140 (= G156), M141 (= M157), A143 (≠ N159), T144 (= T160), A146 (= A162), S147 (= S163), E200 (= E267), G201 (= G268), I206 (= I273), W322 (vs. gap), E413 (= E500), H414 (= H501), N450 (= N536)
binding manganese (ii) ion: H369 (= H456), H376 (= H463), E413 (= E500)

8jdzA Crystal structure of mldhd in complex with 2-keto-3-methylvaleric acid
22% identity, 52% coverage: 18:541/1013 of query aligns to 5:454/454 of 8jdzA

query
sites
8jdzA

Y

F

L

V

D

E

A

A

L

L

A

K

Q

A

S

V

G

V

Y

G

A

S

G

P

D

H

I

V

D

S

K

T

R

A

Y

S

S

A

A

V

R

R

L

E

V

Q

Q

H

A

G

T

H

D

D

N
x
E

S

S

V

M

Y

H

Q

R

F

C

L

Q

P

P

-

P

Q

D

A

A

V

V

L

V

Y

W

P

P

K

Q

H

N

Q

V

K

D

D

Q

I

V

Q

S

I

R

A

V

L

A

S

S

L

L

A

C

A

Y

K

N

A

Q

E

G

F

V

A

P

G

I

V

I

T
x
P

F

F

S
x
G

A
x
T

R
x
G

G
x
T

G
|
G

G

V

T

E

G

G

T
x
G

N
x
V

G

C

Q

-

S

A

L

V

T

Q

H

G

G

G

L

V

I

C

L

I

D

N

V
x
L

S

T

R

-

Y

H

M

M

N

D

R

Q

V

I

L

T

E

E

V

L

N

N

H

T

E

E

Q

D

G

F

W

S

V

V

R

V

V

V

E

E

A

P

G

G

V

V

V

T

K

R

D

K

A

A

L

L

N

N

D

T

A

H

L

L

R

R

P

D

H

S

G

G

Y

L

F

W

F

F

S

P

P

V

D

D

L
x
P

S
x
G

T
x
A

S

D

N

-

R

-

A

A

T

S

I

L

G

C

G
|
G

M
|
M

I

A

N
x
A

T
|
T

D

G

A
|
A

S
|
S

G

G

A

T

G

N

S

A

L

V

V

R

Y

Y

G

G

K

T

T

M

S

R

D

D

H

N

V

V

L

I

A

N

L

L

R

E

S

V

I

V

L

L

I

-

D

-

G

-

S

-

V

-

F

-

D

-

T

-

R

-

P

-

L

-

D

-

A

-

G

-

L

-

C

-

N

-

P

P

D

D

N

G

V

-

S

-

D

-

N

-

P

-

L

-

G

-

Q

-

K

-

L

-

I

-

S

-

A

-

I

-

A

-

E

-

V

-

C

-

R

-

E

-

K

-

R

-

A

-

L

-

I

-

E

-

Q

-

F

-

P

-

K

-

L

-

N

-

R

R

F

L

L

L

T

H

G

T

Y

A

D

G

L

R

K

G

N

R

V

H

W

Y

N

R

N

K

G

S

L

A

T

A

Q

G

F

Y

D

N

L

L

S

T

R

G

I

L

L

F

T

V

G

G

S

S

E
|
E

G
|
G

T

T

L

L

A

G

V

I

I
|
I

T

T

E

S

A

T

K

T

L

L

N

R

I

L

T

H

P

P

L

A

P

P

S

E

E

A

R

T

A

V

M

A

V

A

N

T

I

C

K

A

Y

F

D

P

S

S

F

V

Q

Q

S

A

A

A

L

V

R

D

H

S

A

T

P

V

S

Q

L

I

V

L

A

Q

A

A

R

A

A

V

T

P

V

V

-

A

-

R

V

I

E

E

T

F

V

L

D

D

S

D

K

V

V

M

L

M

N

D

L

A

A

C

R

N

E

R

D

H

I

S

V

L

W

N

H

C

S

P

V

V

S

A

D

P

L

T

I

L

Q

-

E

-

V

-

P

-

G

-

K

-

V

-

I

-

D

-

G

-

L

-

N

-

M

F

V

L

E

E

F

F

A

H

G

G

D

S

A

Q

V

Q

E

T

V

L

S

A

E

E

K

Q

L

L

A

Q

A

R

L

T

E

E

A

A

V

I

L

T

T

Q

E

D

Q

N

I

-

N

-

R

-

G

-

E

-

C

-

G

-

V

-

V

G

G

G

Y

S

Q

H

V

F

T

S

Q

W

D

A

K

A

A

E

S

K

I

R

E

N

K

E

I

L

Y

W

G

A

M

A

R
|
R

K

H

K

N

A

A

-
x
W

V

Y

G

A

L

A

L

L

G

A

A

L

T

S

K

P

G

G

R

S

R

K

K

-

P

-

I

-

A

A

F

Y

A

S

E

T

D

D

T

V

A

C

V

V

P

P

P

I

E

S

M

R

L

L

A

P

D

E

Y

I

I

L

M

V

E

E

F

T

R

K

A

E

L

E

L

I

D

K

S

A

H

S

N

K

L

L

Q

T

Y

G

G

A

M

I

F

V

G

G

H
|
H

V

V

D

G

A

D

G

G

V

N

L

F

H
|
H

V

C

R

I

P

L

A

L

L

V

D

D

M

P

C

-

D

D

V

A

E

E

D

E

E

Q

K

R

L

R

L

V

R

K

V

A

I

F

S

A

D

E

Q

N

V

L

A

G

A

R

L

R

T

A

L

L

K

A

Y

L

G

G

L

T

M

C

W

T

G

G

E
|
E

H
|
H

G

G

K

I

G

G

-

L

-

G

-

K

-

R

-

Q

-

L

V

L

R

Q

G

E

E

E

Y

V

G

G

P

P

S

-

V

-

F

V

G

G

D

V

E

E

L

-

Y

-

G

-

V

T

L

M

Q

R

D

Q

I

L

K

K

G

N

L

T

F

L

D

D

P

P

D

R

N

G

R

L

L

M

N
|
N

P

P

G

G

K

K

L

V

V

L

binding (3S)-3-methyl-2-oxopentanoic acid: R318 (= R403), W322 (vs. gap), H369 (= H456), H376 (= H463), H413 (= H501)
binding flavin-adenine dinucleotide: E32 (≠ N45), P68 (≠ T80), G70 (≠ S82), T71 (≠ A83), G72 (≠ R84), T73 (≠ G85), G74 (= G86), G78 (≠ T90), V79 (≠ N91), L90 (≠ V103), P132 (≠ L146), G133 (≠ S147), A134 (≠ T148), G140 (= G156), M141 (= M157), A143 (≠ N159), T144 (= T160), A146 (= A162), S147 (= S163), E200 (= E267), G201 (= G268), I206 (= I273), W322 (vs. gap), E412 (= E500), H413 (= H501), N449 (= N536)
binding manganese (ii) ion: H369 (= H456), H376 (= H463), E412 (= E500)

8jdyA Crystal structure of mldhd in complex with 2-ketoisocaproic acid
22% identity, 52% coverage: 18:541/1013 of query aligns to 5:454/454 of 8jdyA

query
sites
8jdyA

Y

F

L

V

D

E

A

A

L

L

A

K

Q

A

S

V

G

V

Y

G

A

S

G

P

D

H

I

V

D

S

K

T

R

A

Y

S

S

A

A

V

R

R

L

E

V

Q

Q

H

A

G

T

H

D

D

N

E

S

S

V

M

Y

H

Q

R

F

C

L

Q

P

P

-

P

Q

D

A

A

V

V

L

V

Y

W

P

P

K

Q

H

N

Q

V

K

D

D

Q

I

V

Q

S

I

R

A

V

L

A

S

S

L

L

A

C

A

Y

K

N

A

Q

E

G

F

V

A

P

G

I

V

I

T
x
P

F

F

S
x
G

A
x
T

R
x
G

G
x
T

G
|
G

G

V

T

E

G

G

T
x
G

N
x
V

G

C

Q

-

S

A

L

V

T

Q

H

G

G

G

L

V

I

C

L

I

D

N

V
x
L

S

T

R

-

Y

H

M

M

N

D

R

Q

V

I

L

T

E

E

V

L

N

N

H

T

E

E

Q

D

G

F

W

S

V

V

R

V

V

V

E

E

A

P

G

G

V

V

V

T

K

R

D

K

A

A

L

L

N

N

D

T

A

H

L

L

R

R

P

D

H

S

G

G

Y

L

F

W

F

F

S

P

P

V

D

D

L
x
P

S
x
G

T
x
A

S

D

N

-

R

-

A

A

T

S

I

L

G

C

G
|
G

M
|
M

I

A

N
x
A

T
|
T

D

G

A
|
A

S
|
S

G

G

A

T

G

N

S

A

L

V

V

R

Y

Y

G

G

K

T

T

M

S

R

D

D

H

N

V

V

L

I

A

N

L

L

R

E

S

V

I

V

L

L

I

-

D

-

G

-

S

-

V

-

F

-

D

-

T

-

R

-

P

-

L

-

D

-

A

-

G

-

L

-

C

-

N

-

P

P

D

D

N

G

V

-

S

-

D

-

N

-

P

-

L

-

G

-

Q

-

K

-

L

-

I

-

S

-

A

-

I

-

A

-

E

-

V

-

C

-

R

-

E

-

K

-