SitesBLAST

431:434 CINC→SINS: No rescue in complementation test of the nonphotochemical quenching (NPQ) phenotype observed in disrupted plants.
859 E→K: In soq1-2; high light intensity-dependent and irreversible nonphotochemical quenching (NPQ) due to a decrease in chlorophyll excited-state lifetime.

5olwA 5-fluorotryptophan labeled beta-phosphoglucomutase in an open conformation (see paper)
26% identity, 91% coverage: 5:187/202 of query aligns to 2:188/224 of 5olwA

query
sites
5olwA

F

F

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K

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A

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active site: D8 (= D11), L9 (≠ M12), D10 (= D13), T16 (≠ S19), K45 (≠ A47), S114 (≠ T111), A115 (≠ G112), K145 (= K144), E169 (= E168), D170 (= D169)
binding calcium ion: D8 (= D11), D10 (= D13), P89 (≠ H86), V92 (= V89), E124 (≠ T123), N127 (≠ G126), E169 (= E168), D170 (= D169), S171 (≠ A170)

6qzgA Beta-glucose 1,6-bisphosphonate bound to wild type beta- phosphoglucomutse in an open conformation.
26% identity, 91% coverage: 5:187/202 of query aligns to 2:188/219 of 6qzgA

query
sites
6qzgA

F

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binding 3,7-anhydro-1,2,8-trideoxy-1,8-diphosphono-D-glycero-D-gulo-octitol: D8 (= D11), L9 (≠ M12), D10 (= D13), H20 (= H23), G46 (= G48), S114 (≠ T111), A115 (≠ G112), S116 (≠ T115), K117 (≠ E116), K145 (= K144)
binding magnesium ion: D8 (= D11), D10 (= D13), D170 (= D169)

1z4nA Structure of beta-phosphoglucomutase with inhibitor bound alpha- galactose 1-phosphate cocrystallized with fluoride (see paper)
26% identity, 91% coverage: 5:187/202 of query aligns to 2:188/219 of 1z4nA

query
sites
1z4nA

F

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active site: D8 (= D11), L9 (≠ M12), D10 (= D13), T16 (≠ S19), K45 (≠ A47), S114 (≠ T111), A115 (≠ G112), K145 (= K144), E169 (= E168), D170 (= D169)
binding 1-O-phosphono-alpha-D-galactopyranose: H20 (= H23), W24 (= W27), V47 (= V49), R49 (≠ T51), S116 (≠ T115), K117 (≠ E116), N118 (≠ E117)
binding magnesium ion: D8 (= D11), D10 (= D13), E169 (= E168), D170 (= D169)

6h91A Phosphorylated beta-phosphoglucomutase from lactococcus lactis in an open conformer to 2.4 a
26% identity, 91% coverage: 5:187/202 of query aligns to 2:188/218 of 6h91A

query
sites
6h91A

F

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binding magnesium ion: D8 (= D11), D10 (= D13), D170 (= D169)
binding acetylphosphate: R49 (≠ T51), K117 (≠ E116)

4c4rA Structure of beta-phosphoglucomutase in complex with a phosphonate analogue of beta-glucose-1-phosphate and magnesium trifluoride (see paper)
26% identity, 91% coverage: 5:187/202 of query aligns to 2:188/218 of 4c4rA

query
sites
4c4rA

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active site: D8 (= D11), L9 (≠ M12), D10 (= D13), T16 (≠ S19), K45 (≠ A47), S114 (≠ T111), A115 (≠ G112), K145 (= K144), E169 (= E168), D170 (= D169)
binding magnesium ion: D8 (= D11), D10 (= D13), D170 (= D169)
binding trifluoromagnesate: D8 (= D11), L9 (≠ M12), D10 (= D13), S114 (≠ T111), A115 (≠ G112), K145 (= K144)
binding (1R)-1,5-anhydro-1-(phosphonomethyl)-D-glucitol: D10 (= D13), H20 (= H23), W24 (= W27), L44 (= L46), G46 (= G48), V47 (= V49), R49 (≠ T51), S52 (≠ T54), S116 (≠ T115), K117 (≠ E116)

3zi4A The structure of beta-phosphoglucomutase inhibited with glucose-6- phosphate and scandium tetrafluoride
26% identity, 91% coverage: 5:187/202 of query aligns to 2:188/218 of 3zi4A

query
sites
3zi4A

F

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E

C

S

I

I

V

G

F

L

E
|
E

D
|
D

A

S

K

Q

A

A

G

G

I

I

Q

Q

A

A

A

I

E

K

A

D

A

S

G

G

M

A

F

L

V

P

V

I

D

G

V

V

active site: D8 (= D11), L9 (≠ M12), D10 (= D13), T16 (≠ S19), K45 (≠ A47), S114 (≠ T111), A115 (≠ G112), K145 (= K144), E169 (= E168), D170 (= D169)
binding 6-O-phosphono-beta-D-glucopyranose: D10 (= D13), H20 (= H23), G46 (= G48), V47 (= V49), R49 (≠ T51), S116 (≠ T115), K117 (≠ E116)
binding magnesium ion: D8 (= D11), D10 (= D13), D170 (= D169)
binding Scandium Tetrafluoride: D8 (= D11), L9 (≠ M12), D10 (= D13), S114 (≠ T111), A115 (≠ G112), K145 (= K144)

2wf8A Structure of beta-phosphoglucomutase inhibited with glucose-6- phosphate, glucose-1-phosphate and beryllium trifluoride (see paper)
26% identity, 91% coverage: 5:187/202 of query aligns to 2:188/218 of 2wf8A

query
sites
2wf8A

F

F

D

K

G

A

I

V

I

L

F

F

D
|
D

M
x
L

D
|
D

G

G

T

V

L

I

V

T

D

D

S
x
T

G

A

Q

E

L

Y

H
|
H

E

F

Q

R

A

A

W
|
W

R

K

Q

A

T

L

L

A

N

E

H

E

F

I

G

G

I

I

P

N

-

G

V

V

E

D

P

R

A

Q

L

F

M

N

R

E

S

Q

L
|
L

A
x
K

G
|
G

V
|
V

P

S

T
x
R

I

E

G

D

T
x
S

L

L

E

Q

I

K

L

I

I

L

A

D

H

L

F

A

G

D

V

K

T

K

P

V

T

S

A

A

S

E

-

E

-

F

-

K

-

E

-

L

C

A

E

K

A

R

M

K

N

N

E

D

Y

N

K

Y

E

V

A

K

L

M

V

I

R

Q

D

D

T

V

M

S

H

P

L

A

Y

D

V

V

K

Y

P

P

T

G

A

I

L

L

A

Q

E

L

F

L

A

K

K

D

Q

L

N

R

Q

S

G

N

K

K

R

I

P

K

M

I

A

A

V

L

G

A

T
x
S

G
x
A

A

-

Y

-

T
x
S

E
x
K

E

N

A

G

I

P

Q

F

I

L

L

L

T

E

L

R

C

M

G

N

L

L

L

T

E

G

L

Y

V

F

D

D

Y

A

V

I

V

A

G

D

A

P

D

A

Q

E

V

V

A

A

S

A

P

S

K
|
K

P

P

A

A

P

P

D

D

T

I

F

F

L

I

R

A

C

A

A

A

E

H

L

A

M

V

G

G

I

V

A

A

P

P

E

S

R

E

C

S

I

I

V

G

F

L

E
|
E

D
|
D

A

S

K

Q

A

A

G

G

I

I

Q

Q

A

A

A

I

E

K

A

D

A

S

G

G

M

A

F

L

V

P

V

I

D

G

V

V

active site: D8 (= D11), L9 (≠ M12), D10 (= D13), T16 (≠ S19), K45 (≠ A47), S114 (≠ T111), A115 (≠ G112), K145 (= K144), E169 (= E168), D170 (= D169)
binding beryllium trifluoride ion: D8 (= D11), L9 (≠ M12), D10 (= D13), S114 (≠ T111), A115 (≠ G112), K145 (= K144)
binding 6-O-phosphono-beta-D-glucopyranose: D10 (= D13), H20 (= H23), G46 (= G48), V47 (= V49), R49 (≠ T51), A115 (≠ G112), S116 (≠ T115), K117 (≠ E116)
binding 1-O-phosphono-alpha-D-glucopyranose: D10 (= D13), H20 (= H23), W24 (= W27), L44 (= L46), G46 (= G48), V47 (= V49), R49 (≠ T51), S52 (≠ T54), A115 (≠ G112), S116 (≠ T115), K117 (≠ E116)
binding magnesium ion: D8 (= D11), D10 (= D13), D170 (= D169)

2wf7A Structure of beta-phosphoglucomutase inhibited with glucose-6- phosphonate and aluminium tetrafluoride (see paper)
26% identity, 91% coverage: 5:187/202 of query aligns to 2:188/218 of 2wf7A

query
sites
2wf7A

F

F

D

K

G

A

I

V

I

L

F

F

D
|
D

M
x
L

D
|
D

G

G

T

V

L

I

V

T

D

D

S
x
T

G

A

Q

E

L

Y

H

H

E

F

Q

R

A

A

W

W

R

K

Q

A

T

L

L

A

N

E

H

E

F

I

G

G

I

I

P

N

-

G

V

V

E

D

P

R

A

Q

L

F

M

N

R

E

S

Q

L

L

A
x
K

G
|
G

V
|
V

P

S

T
x
R

I

E

G

D

T

S

L

L

E

Q

I

K

L

I

I

L

A

D

H

L

F

A

G

D

V

K

T

K

P

V

T

S

A

A

S

E

-

E

-

F

-

K

-

E

-

L

C

A

E

K

A

R

M

K

N

N

E

D

Y

N

K

Y

E

V

A

K

L

M

V

I

R

Q

D

D

T

V

M

S

H

P

L

A

Y

D

V

V

K

Y

P

P

T

G

A

I

L

L

A

Q

E

L

F

L

A

K

K

D

Q

L

N

R

Q

S

G

N

K

K

R

I

P

K

M

I

A

A

V

L

G

A

T
x
S

G
x
A

A

-

Y

-

T
x
S

E
x
K

E
x
N

A

G

I

P

Q

F

I

L

L

L

T

E

L

R

C

M

G

N

L

L

L

T

E

G

L

Y

V

F

D

D

Y

A

V

I

V

A

G

D

A

P

D

A

Q

E

V

V

A

A

S

A

P

S

K
|
K

P

P

A

A

P

P

D

D

T

I

F

F

L

I

R

A

C

A

A

A

E

H

L

A

M

V

G

G

I

V

A

A

P

P

E

S

R

E

C

S

I

I

V

G

F

L

E
|
E

D
|
D

A

S

K

Q

A

A

G

G

I

I

Q

Q

A

A

A

I

E

K

A

D

A

S

G

G

M

A

F

L

V

P

V

I

D

G

V

V

active site: D8 (= D11), L9 (≠ M12), D10 (= D13), T16 (≠ S19), K45 (≠ A47), S114 (≠ T111), A115 (≠ G112), K145 (= K144), E169 (= E168), D170 (= D169)
binding tetrafluoroaluminate ion: D8 (= D11), L9 (≠ M12), D10 (= D13), S114 (≠ T111), K145 (= K144)
binding 6,7-dideoxy-7-phosphono-beta-D-gluco-heptopyranose: D10 (= D13), G46 (= G48), V47 (= V49), R49 (≠ T51), S116 (≠ T115), K117 (≠ E116), N118 (≠ E117)
binding magnesium ion: D8 (= D11), D10 (= D13), D170 (= D169)

2wf6A Structure of beta-phosphoglucomutase inhibited with glucose-6- phosphate and aluminium tetrafluoride (see paper)
26% identity, 91% coverage: 5:187/202 of query aligns to 2:188/218 of 2wf6A

query
sites
2wf6A

F

F

D

K

G

A

I

V

I

L

F

F

D
|
D

M
x
L

D
|
D

G

G

T

V

L

I

V

T

D

D

S
x
T

G

A

Q

E

L

Y

H

H

E

F

Q

R

A

A

W

W

R

K

Q

A

T

L

L

A

N

E

H

E

F

I

G

G

I

I

P

N

-

G

V

V

E

D

P

R

A

Q

L

F

M

N

R

E

S

Q

L

L

A
x
K

G
|
G

V
|
V

P

S

T
x
R

I

E

G

D

T

S

L

L

E

Q

I

K

L

I

I

L

A

D

H

L

F

A

G

D

V

K

T

K

P

V

T

S

A

A

S

E

-

E

-

F

-

K

-

E

-

L

C

A

E

K

A

R

M

K

N

N

E

D

Y

N

K

Y

E

V

A

K

L

M

V

I

R

Q

D

D

T

V

M

S

H

P

L

A

Y

D

V

V

K

Y

P

P

T

G

A

I

L

L

A

Q

E

L

F

L

A

K

K

D

Q

L

N

R

Q

S

G

N

K

K

R

I

P

K

M

I

A

A

V

L

G

A

T
x
S

G
x
A

A

-

Y

-

T
x
S

E
x
K

E

N

A

G

I

P

Q

F

I

L

L

L

T

E

L

R

C

M

G

N

L

L

L

T

E

G

L

Y

V

F

D

D

Y

A

V

I

V

A

G

D

A

P

D

A

Q

E

V

V

A

A

S

A

P

S

K
|
K

P

P

A

A

P

P

D

D

T

I

F

F

L

I

R

A

C

A

A

A

E

H

L

A

M

V

G

G

I

V

A

A

P

P

E

S

R

E

C

S

I

I

V

G

F

L

E
|
E

D
|
D

A

S

K

Q

A

A

G

G

I

I

Q

Q

A

A

A

I

E

K

A

D

A

S

G

G

M

A

F

L

V

P

V

I

D

G

V

V

active site: D8 (= D11), L9 (≠ M12), D10 (= D13), T16 (≠ S19), K45 (≠ A47), S114 (≠ T111), A115 (≠ G112), K145 (= K144), E169 (= E168), D170 (= D169)
binding tetrafluoroaluminate ion: D8 (= D11), L9 (≠ M12), D10 (= D13), S114 (≠ T111), K145 (= K144)
binding 6-O-phosphono-beta-D-glucopyranose: D10 (= D13), G46 (= G48), V47 (= V49), R49 (≠ T51), S116 (≠ T115), K117 (≠ E116)
binding magnesium ion: D8 (= D11), D10 (= D13), D170 (= D169)

2wf5A Structure of beta-phosphoglucomutase inhibited with glucose-6- phosphate and trifluoromagnesate (see paper)
26% identity, 91% coverage: 5:187/202 of query aligns to 2:188/218 of 2wf5A

query
sites
2wf5A

F

F

D

K

G

A

I

V

I

L

F

F

D
|
D

M
x
L

D
|
D

G

G

T

V

L

I

V

T

D

D

S
x
T

G

A

Q

E

L

Y

H
|
H

E

F

Q

R

A

A

W

W

R

K

Q

A

T

L

L

A

N

E

H

E

F

I

G

G

I

I

P

N

-

G

V

V

E

D

P

R

A

Q

L

F

M

N

R

E

S

Q

L

L

A
x
K

G
|
G

V
|
V

P

S

T
x
R

I

E

G

D

T

S

L

L

E

Q

I

K

L

I

I

L

A

D

H

L

F

A

G

D

V

K

T

K

P

V

T

S

A

A

S

E

-

E

-

F

-

K

-

E

-

L

C

A

E

K

A

R

M

K

N

N

E

D

Y

N

K

Y

E

V

A

K

L

M

V

I

R

Q

D

D

T

V

M

S

H

P

L

A

Y

D

V

V

K

Y

P

P

T

G

A

I

L

L

A

Q

E

L

F

L

A

K

K

D

Q

L

N

R

Q

S

G

N

K

K

R

I

P

K

M

I

A

A

V

L

G

A

T
x
S

G
x
A

A

-

Y

-

T
x
S

E

K

E

N

A

G

I

P

Q

F

I

L

L

L

T

E

L

R

C

M

G

N

L

L

L

T

E

G

L

Y

V

F

D

D

Y

A

V

I

V

A

G

D

A

P

D

A

Q

E

V

V

A

A

S

A

P

S

K
|
K

P

P

A

A

P

P

D

D

T

I

F

F

L

I

R

A

C

A

A

A

E

H

L

A

M

V

G

G

I

V

A

A

P

P

E

S

R

E

C

S

I

I

V

G

F

L

E
|
E

D
|
D

A

S

K

Q

A

A

G

G

I

I

Q

Q

A

A

A

I

E

K

A

D

A

S

G

G

M

A

F

L

V

P

V

I

D

G

V

V

active site: D8 (= D11), L9 (≠ M12), D10 (= D13), T16 (≠ S19), K45 (≠ A47), S114 (≠ T111), A115 (≠ G112), K145 (= K144), E169 (= E168), D170 (= D169)
binding 6-O-phosphono-beta-D-glucopyranose: D10 (= D13), H20 (= H23), G46 (= G48), V47 (= V49), R49 (≠ T51), A115 (≠ G112), S116 (≠ T115)
binding magnesium ion: D8 (= D11), D10 (= D13), D170 (= D169)
binding trifluoromagnesate: D8 (= D11), L9 (≠ M12), D10 (= D13), S114 (≠ T111), A115 (≠ G112), K145 (= K144)

2wf9A Structure of beta-phosphoglucomutase inhibited with glucose-6- phosphate, and beryllium trifluoride, crystal form 2 (see paper)
26% identity, 91% coverage: 5:187/202 of query aligns to 2:188/221 of 2wf9A

query
sites
2wf9A

F

F

D

K

G

A

I

V

I

L

F

F

D
|
D

M
x
L

D
|
D

G

G

T

V

L

I

V

T

D

D

S
x
T

G

A

Q

E

L

Y

H
|
H

E

F

Q

R

A

A

W

W

R

K

Q

A

T

L

L

A

N

E

H

E

F

I

G

G

I

I

P

N

-

G

V

V

E

D

P

R

A

Q

L

F

M

N

R

E

S

Q

L

L

A
x
K

G
|
G

V
|
V

P

S

T
x
R

I

E

G

D

T

S

L

L

E

Q

I

K

L

I

I

L

A

D

H

L

F

A

G

D

V

K

T

K

P

V

T

S

A

A

S

E

-

E

-

F

-

K

-

E

-

L

C

A

E

K

A

R

M

K

N

N

E

D

Y

N

K

Y

E

V

A

K

L

M

V

I

R

Q

D

D

T

V

M

S

H

P

L

A

Y

D

V

V

K

Y

P

P

T

G

A

I

L

L

A

Q

E

L

F

L

A

K

K

D

Q

L

N

R

Q

S

G

N

K

K

R

I

P

K

M

I

A

A

V

L

G

A

T
x
S

G
x
A

A

-

Y

-

T
x
S

E
x
K

E
x
N

A

G

I

P

Q

F

I

L

L

L

T

E

L

R

C

M

G

N

L

L

L

T

E

G

L

Y

V

F

D

D

Y

A

V

I

V

A

G

D

A

P

D

A

Q

E

V

V

A

A

S

A

P

S

K
|
K

P

P

A

A

P

P

D

D

T

I

F

F

L

I

R

A

C

A

A

A

E

H

L

A

M

V

G

G

I

V

A

A

P

P

E

S

R

E

C

S

I

I

V

G

F

L

E
|
E

D
|
D

A

S

K

Q

A

A

G

G

I

I

Q

Q

A

A

A

I

E

K

A

D

A

S

G

G

M

A

F

L

V

P

V

I

D

G

V

V

active site: D8 (= D11), L9 (≠ M12), D10 (= D13), T16 (≠ S19), K45 (≠ A47), S114 (≠ T111), A115 (≠ G112), K145 (= K144), E169 (= E168), D170 (= D169)
binding beryllium trifluoride ion: D8 (= D11), L9 (≠ M12), D10 (= D13), S114 (≠ T111), A115 (≠ G112), K145 (= K144)
binding 6-O-phosphono-beta-D-glucopyranose: D10 (= D13), H20 (= H23), G46 (= G48), V47 (= V49), R49 (≠ T51), S116 (≠ T115), K117 (≠ E116), N118 (≠ E117)
binding 6-O-phosphono-alpha-D-glucopyranose: D10 (= D13), H20 (= H23), G46 (= G48), V47 (= V49), R49 (≠ T51), A115 (≠ G112), S116 (≠ T115), K117 (≠ E116), N118 (≠ E117)
binding magnesium ion: D8 (= D11), D10 (= D13), D170 (= D169)

1o03A Structure of pentavalent phosphorous intermediate of an enzyme catalyzed phosphoryl transfer reaction observed on cocrystallization with glucose 6-phosphate (see paper)
26% identity, 91% coverage: 5:187/202 of query aligns to 2:188/221 of 1o03A

query
sites
1o03A

F

F

D

K

G

A

I

V

I

L

F

F

D
|
D

M
x
L

D
|
D

G

G

T

V

L

I

V

T

D

D

S
x
T

G

A

Q

E

L

Y

H
|
H

E

F

Q

R

A

A

W

W

R

K

Q

A

T

L

L

A

N

E

H

E

F

I

G

G

I

I

P

N

-

G

V

V

E

D

P

R

A

Q

L

F

M

N

R

E

S

Q

L

L

A
x
K

G
|
G

V
|
V

P

S

T
x
R

I

E

G

D

T

S

L

L

E

Q

I

K

L

I

I

L

A

D

H

L

F

A

G

D

V

K

T

K

P

V

T

S

A

A

S

E

-

E

-

F

-

K

-

E

-

L

C

A

E

K

A

R

M

K

N

N

E

D

Y

N

K

Y

E

V

A

K

L

M

V

I

R

Q

D

D

T

V

M

S

H

P

L

A

Y

D

V

V

K

Y

P

P

T

G

A

I

L

L

A

Q

E

L

F

L

A

K

K

D

Q

L

N

R

Q

S

G

N

K

K

R

I

P

K

M

I

A

A

V

L

G

A

T
x
S

G
x
A

A

-

Y

-

T
x
S

E
x
K

E

N

A

G

I

P

Q

F

I

L

L

L

T

E

L

R

C

M

G

N

L

L

L

T

E

G

L

Y

V

F

D

D

Y

A

V

I

V

A

G

D

A

P

D

A

Q

E

V

V

A

A

S

A

P

S

K
|
K

P

P

A

A

P

P

D

D

T

I

F

F

L

I

R

A

C

A

A

A

E

H

L

A

M

V

G

G

I

V

A

A

P

P

E

S

R

E

C

S

I

I

V

G

F

L

E
|
E

D
|
D

A

S

K

Q

A

A

G

G

I

I

Q

Q

A

A

A

I

E

K

A

D

A

S

G

G

M

A

F

L

V

P

V

I

D

G

V

V

active site: D8 (= D11), L9 (≠ M12), D10 (= D13), T16 (≠ S19), K45 (≠ A47), S114 (≠ T111), A115 (≠ G112), K145 (= K144), E169 (= E168), D170 (= D169)
binding 1,6-di-O-phosphono-alpha-D-glucopyranose: D8 (= D11), L9 (≠ M12), D10 (= D13), H20 (= H23), G46 (= G48), V47 (= V49), R49 (≠ T51), S114 (≠ T111), A115 (≠ G112), S116 (≠ T115), K117 (≠ E116), K145 (= K144)
binding magnesium ion: D8 (= D11), D10 (= D13), D170 (= D169)

1lvhA The structure of phosphorylated beta-phosphoglucomutase from lactoccocus lactis to 2.3 angstrom resolution (see paper)
26% identity, 91% coverage: 5:187/202 of query aligns to 2:188/221 of 1lvhA

query
sites
1lvhA

F

F

D

K

G

A

I

V

I

L

F

F

D
|
D

M
x
L

D
|
D

G

G

T

V

L

I

V

T

D

D

S
x
T

G

A

Q

E

L

Y

H

H

E

F

Q

R

A

A

W

W

R

K

Q

A

T

L

L

A

N

E

H

E

F

I

G

G

I

I

P

N

-

G

V

V

E

D

P

R

A

Q

L

F

M

N

R

E

S

Q

L

L

A
x
K

G

G

V

V

P

S

T

R

I

E

G

D

T

S

L

L

E

Q

I

K

L

I

I

L

A

D

H

L

F

A

G

D

V

K

T

K

P

V

T

S

A

A

S

E

-

E

-

F

-

K

-

E

-

L

C

A

E

K

A

R

M

K

N

N

E

D

Y

N

K

Y

E

V

A

K

L

M

V

I

R

Q

D

D

T

V

M

S

H

P

L

A

Y

D

V

V

K

Y

P

P

T

G

A

I

L

L

A

Q

E

L

F

L

A

K

K

D

Q

L

N

R

Q

S

G

N

K

K

R

I

P

K

M

I

A

A

V

L

G

A

T
x
S

G
x
A

A

-

Y

-

T

S

E

K

E

N

A

G

I

P

Q

F

I

L

L

L

T

E

L

R

C

M

G

N

L

L

L

T

E

G

L

Y

V

F

D

D

Y

A

V

I

V

A

G

D

A

P

D

A

Q

E

V

V

A

A

S

A

P

S

K
|
K

P

P

A

A

P

P

D

D

T

I

F

F

L

I

R

A

C

A

A

A

E

H

L

A

M

V

G

G

I

V

A

A

P

P

E

S

R

E

C

S

I

I

V

G

F

L

E
|
E

D
|
D

A

S

K

Q

A

A

G

G

I

I

Q

Q

A

A

A

I

E

K

A

D

A

S

G

G

M

A

F

L

V

P

V

I

D

G

V

V

active site: D8 (= D11), L9 (≠ M12), D10 (= D13), T16 (≠ S19), K45 (≠ A47), S114 (≠ T111), A115 (≠ G112), K145 (= K144), E169 (= E168), D170 (= D169)
binding magnesium ion: D8 (= D11), D10 (= D13), D170 (= D169)

P71447 Beta-phosphoglucomutase; Beta-PGM; EC 5.4.2.6 from Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) (see 3 papers)
26% identity, 91% coverage: 5:187/202 of query aligns to 2:188/221 of P71447

query
sites
P71447

F

F

D

K

G

A

I

V

I

L

F

F

D
|
D

M

L

D
|
D

G

G

T

V

L

I

V

T

D

D

S
x
T

G

A

Q

E

L

Y

H
|
H

E

F

Q

R

A

A

W

W

R

K

Q

A

T

L

L

A

N

E

H

E

F

I

G

G

I

I

P

N

-

G

V

V

E

D

P

R

A

Q

L

F

M

N

R

E

S

Q

L

L

A
x
K

G
|
G

V

V

P

S

T
x
R

I

E

G

D

T
x
S

L

L

E

Q

I

K

L

I

I

L

A

D

H

L

F

A

G

D

V

K

T

K

P

V

T

S

A

A

S

E

-

E

-

F

-

K

-

E

-

L

C

A

E

K

A

R

M
x
K

N

N

E

D

Y

N

K

Y

E

V

A

K

L

M

V

I

R

Q

D

D

T

V

M

S

H

P

L

A

Y

D

V

V

K

Y

P

P

T

G

A

I

L

L

A

Q

E

L

F

L

A

K

K

D

Q

L

N

R

Q

S

G

N

K

K

R

I

P

K

M

I

A

A

V

L

G

A

T

S

G

A

A

-

Y

-

T

S

E

K

E

N

A

G

I

P

Q

F

I

L

L

L

T

E

L

K

C

M

G

N

L

L

L

T

E

G

L

Y

V

F

D

D

Y

A

V

I

V

A

G

D

A

P

D

A

Q

E

V

V

A

A

S

A

P

S

K

K

P

P

A

A

P

P

D

D

T

I

F

F

L

I

R

A

C

A

A

A

E

H

L

A

M

V

G

G

I

V

A

A

P

P

E

S

R

E

C

S

I

I

V

G

F

L

E

E

D
|
D

A

S

K

Q

A

A

G

G

I

I

Q

Q

A

A

A

I

E

K

A

D

A

S

G

G

M

A

F

L

V

P

V

I

D

G

V

V

D8 (= D11) modified: 4-aspartylphosphate; mutation to A: Inactive.; mutation to E: Inactive.
D10 (= D13) mutation to A: Inactive.; mutation to E: Inactive.; mutation to N: Inactive.; mutation to S: Inactive.
T16 (≠ S19) mutation to P: 500-fold reduction in the rate constant for Asp-8 phosphorylation by beta-G1,6bisP. 6,700-fold reduction in the apparent rate constant for cycling of the phosphorylated enzyme to convert beta-G1P to G6P. 13-fold increase in the estimated rate constant for phosphoryl transfer from the phospho-Asp8 to water.
H20 (= H23) mutation to A: Impairs Asp-8 phosphorylation by beta-G1,6bisP and phosphoryl transfer from the phospho-Asp8 to the substrate beta-G1P.; mutation to N: 300-fold reduction in the conversion of beta-G1P to G6P in the presence of beta-G1,6bisP.; mutation to Q: 8-fold reduction in the conversion of beta-G1P to G6P in the presence of beta-G1,6bisP.
K45 (≠ A47) mutation to A: 20'000-fold decrease in kcat/KM.
G46 (= G48) mutation to A: 1'000'000-fold decrease in kcat/KM.; mutation to P: 100'000-fold decrease in kcat/KM.; mutation to V: 10'000-fold decrease in kcat/KM.
R49 (≠ T51) mutation to K: 1'000'000-fold decrease in kcat/KM.
S52 (≠ T54) mutation to A: Wild-type activity.
K76 (≠ M73) mutation to A: 100-fold reduction in the conversion of beta-G1P to G6P in the presence of beta-G1,6bisP.
D170 (= D169) mutation to A: Impaired, but active with an increase in the affinity for G1P.

4c4sA Structure of beta-phosphoglucomutase in complex with an alpha- fluorophosphonate analogue of beta-glucose-1-phosphate and magnesium trifluoride (see paper)
27% identity, 91% coverage: 5:187/202 of query aligns to 2:185/215 of 4c4sA

query
sites
4c4sA

F

F

D

K

G

A

I

V

I

L

F

F

D
|
D

M
x
L

D
|
D

G

G

T

V

L

I

V

T

D

D

S
x
T

G

A

Q

E

L

Y

H
|
H

E

F

Q

R

A

A

W
|
W

R

K

Q

A

T

L

L

A

N

E

H

E

F

I

G

G

I

I

P

N

-

G

V

V

E

D

P

R

A

Q

L

F

M

N

R

E

S

Q

L
|
L

A
x
K

G
|
G

V
|
V

P

S

T
x
R

I

E

G

D

T

S

L

L

E

Q

I

K

L

I

I

L

A

-

H

D

F

L

G

K

V

V

T

S

P

A

T

E

A

E

S

F

C

K

E

E

-

L

-

A

-

K

A

R

M

K

N

N

E

D

Y

N

K

Y

E

V

A

K

L

M

V

I

R

Q

D

D

T

V

M

S

H

P

L

A

Y

D

V

V

K

Y

P

P

T

G

A

I

L

L

A

Q

E

L

F

L

A

K

K

D

Q

L

N

R

Q

S

G

N

K

K

R

I

P

K

M

I

A

A

V

L

G

A

T
x
S

G
x
A

A

-

Y

-

T
x
S

E

K

E

N

A

G

I

P

Q

F

I

L

L

L

T

E

L

R

C

M

G

N

L

L

L

T

E

G

L

Y

V

F

D

D

Y

A

V

I

V

A

G

D

A

P

D

A

Q

E

V

V

A

A

S

A

P

S

K
|
K

P

P

A

A

P

P

D

D

T

I

F

F

L

I

R

A

C

A

A

A

E

H

L

A

M

V

G

G

I

V

A

A

P

P

E

S

R

E

C

S

I

I

V

G

F

L

E
|
E

D
|
D

A

S

K

Q

A

A

G

G

I

I

Q

Q

A

A

A

I

E

K

A

D

A

S

G

G

M

A

F

L

V

P

V

I

D

G

V

V

active site: D8 (= D11), L9 (≠ M12), D10 (= D13), T16 (≠ S19), K45 (≠ A47), S111 (≠ T111), A112 (≠ G112), K142 (= K144), E166 (= E168), D167 (= D169)
binding (1R)-1,5-anhydro-1-[(S)-fluoro(phosphono)methyl]-D-glucitol: D10 (= D13), H20 (= H23), W24 (= W27), L44 (= L46), G46 (= G48), V47 (= V49), R49 (≠ T51), S113 (≠ T115)
binding magnesium ion: D8 (= D11), D10 (= D13), D167 (= D169)
binding trifluoromagnesate: D8 (= D11), L9 (≠ M12), D10 (= D13), S111 (≠ T111), A112 (≠ G112), K142 (= K144)

5ok0A Structure of the d10n mutant of beta-phosphoglucomutase from lactococcus lactis trapped with native reaction intermediate beta- glucose 1,6-bisphosphate to 2.2a resolution.
25% identity, 91% coverage: 5:187/202 of query aligns to 2:188/218 of 5ok0A

query
sites
5ok0A

F

F

D

K

G

A

I

V

I

L

F

F

D
|
D

M
x
L

D
x
N

G

G

T

V

L

I

V

T

D

D

S
x
T

G

A

Q

E

L

Y

H
|
H

E

F

Q

R

A

A

W

W

R

K

Q

A

T

L

L

A

N

E

H

E

F

I

G

G

I
|
I

P
x
N

-
x
G

V
|
V

E

D

P

R

A

Q

L

F

M

N

R

E

S

Q

L
|
L

A
x
K

G

G

V
|
V

P

S

T
x
R

I

E

G

D

T

S

L

L

E

Q

I

K

L

I

I

L

A

D

H

L

F

A

G

D

V

K

T

K

P

V

T

S

A

A

S

E

-

E

-

F

-

K

-

E

-

L

C

A

E

K

A

R

M

K

N

N

E

D

Y

N

K

Y

E

V

A

K

L

M

V

I

R

Q

D

D

T

V

M

S

H

P

L

A

Y

D

V

V

K

Y

P

P

T

G

A

I

L

L

A

Q

E

L

F

L

A

K

K

D

Q

L

N

R

Q

S

G

N

K

K

R

I

P

K

M

I

A

A

V

L

G

A

T
x
S

G
x
A

A

-

Y

-

T
x
S

E

K

E

N

A

G

I

P

Q

F

I

L

L

L

T

E

L

R

C

M

G

N

L

L

L

T

E

G

L

Y

V

F

D

D

Y

A

V

I

V

A

G

D

A

P

D

A

Q

E

V

V

A

A

S

A

P

S

K
|
K

P

P

A

A

P

P

D

D

T

I

F

F

L

I

R

A

C

A

A

A

E

H

L

A

M

V

G

G

I

V

A

A

P

P

E

S

R

E

C

S

I

I

V

G

F

L

E
|
E

D
|
D

A

S

K

Q

A

A

G

G

I

I

Q

Q

A

A

A

I

E

K

A

D

A

S

G

G

M

A

F

L

V

P

V

I

D

G

V

V

active site: D8 (= D11), L9 (≠ M12), N10 (≠ D13), T16 (≠ S19), K45 (≠ A47), S114 (≠ T111), A115 (≠ G112), K145 (= K144), E169 (= E168), D170 (= D169)
binding 1,6-di-O-phosphono-beta-D-glucopyranose: D8 (= D11), L9 (≠ M12), N10 (≠ D13), H20 (= H23), L44 (= L46), V47 (= V49), R49 (≠ T51), S114 (≠ T111), A115 (≠ G112), S116 (≠ T115)
binding magnesium ion: D8 (= D11), N10 (≠ D13), D170 (= D169)
binding 1,3-propandiol: I33 (= I36), N34 (≠ P37), G35 (vs. gap), V36 (= V38)

5o6rA Structure of beta-phosphoglucomutase d10n mutant in complex with glucose-1-phosphate and aluminium tetrafluoride
25% identity, 91% coverage: 5:187/202 of query aligns to 2:188/218 of 5o6rA

query
sites
5o6rA

F

F

D

K

G

A

I

V

I

L

F

F

D
|
D

M
x
L

D
x
N

G

G

T

V

L

I

V

T

D

D

S
x
T

G

A

Q

E

L

Y

H
|
H

E

F

Q

R

A

A

W
|
W

R

K

Q

A

T

L

L

A

N

E

H

E

F

I

G

G

I

I

P

N

-

G

V

V

E

D

P

R

A

Q

L

F

M

N

R

E

S

Q

L
|
L

A
x
K

G
|
G

V
|
V

P

S

T
x
R

I

E

G

D

T
x
S

L

L

E

Q

I

K

L

I

I

L

A

D

H

L

F

A

G

D

V

K

T

K

P

V

T

S

A

A

S

E

-

E

-

F

-

K

-

E

-

L

C

A

E

K

A

R

M

K

N

N

E

D

Y

N

K

Y

E

V

A

K

L

M

V

I

R

Q

D

D

T

V

M

S

H

P

L

A

Y

D

V

V

K

Y

P

P

T

G

A

I

L

L

A

Q

E

L

F

L

A

K

K

D

Q

L

N

R

Q

S

G

N

K

K

R

I

P

K

M

I

A

A

V

L

G

A

T
x
S

G
x
A

A

-

Y

-

T
x
S

E
x
K

E
x
N

A

G

I

P

Q

F

I

L

L

L

T

E

L

R

C

M

G

N

L

L

L

T

E

G

L

Y

V

F

D

D

Y

A

V

I

V

A

G

D

A

P

D

A

Q

E

V

V

A

A

S

A

P

S

K
|
K

P

P

A

A

P

P

D

D

T

I

F

F

L

I

R

A

C

A

A

A

E

H

L

A

M

V

G

G

I

V

A

A

P

P

E

S

R

E

C

S

I

I

V

G

F

L

E
|
E

D
|
D

A

S

K

Q

A

A

G

G

I

I

Q

Q

A

A

A

I

E

K

A

D

A

S

G

G

M

A

F

L

V

P

V

I

D

G

V

V

active site: D8 (= D11), L9 (≠ M12), N10 (≠ D13), T16 (≠ S19), K45 (≠ A47), S114 (≠ T111), A115 (≠ G112), K145 (= K144), E169 (= E168), D170 (= D169)
binding tetrafluoroaluminate ion: D8 (= D11), L9 (≠ M12), N10 (≠ D13), G46 (= G48), S114 (≠ T111), K145 (= K144)
binding magnesium ion: D8 (= D11), N10 (≠ D13), D170 (= D169)
binding 1-O-phosphono-beta-D-glucopyranose: H20 (= H23), W24 (= W27), L44 (= L46), V47 (= V49), R49 (≠ T51), S52 (≠ T54), S116 (≠ T115), K117 (≠ E116), N118 (≠ E117)

5o6pA Structure of beta-phosphoglucomutase d10n mutant in complex with glucose-1,6-bisphosphate
25% identity, 91% coverage: 5:187/202 of query aligns to 1:187/209 of 5o6pA

query
sites
5o6pA

F

F

D

K

G

A

I

V

I

L

F

F

D
|
D

M
x
L

D
x
N

G

G

T

V

L

I

V

T

D

D

S
x
T

G

A

Q

E

L

Y

H
|
H

E

F

Q

R

A

A

W

W

R

K

Q

A

T

L

L

A

N

E

H

E

F

I

G

G

I

I

P

N

-

G

V

V

E

D

P

R

A

Q

L

F

M

N

R

E

S

Q

L
|
L

A
x
K

G
|
G

V
|
V

P

S

T
x
R

I

E

G

D

T

S

L

L

E

Q

I

K

L

I

I

L

A

D

H

L

F

A

G

D

V

K

T

K

P

V

T

S

A

A

S

E

-

E

-

F

-

K

-

E

-

L

C

A

E

K

A

R

M

K

N

N

E

D

Y

N

K

Y

E

V

A

K

L

M

V

I

R

Q

D

D

T

V

M

S

H

P

L

A

Y

D

V

V

K

Y

P

P

T

G

A

I

L

L

A

Q

E

L

F

L

A

K

K

D

Q

L

N

R

Q

S

G

N

K

K

R

I

P

K

M

I

A

A

V

L

G

A

T
x
S

G
x
A

A

-

Y

-

T
x
S

E
x
K

E

N

A

G

I

P

Q

F

I

L

L

L

T

E

L

R

C

M

G

N

L

L

L

T

E

G

L

Y

V

F

D

D

Y

A

V

I

V

A

G

D

A

P

D

A

Q

E

V

V

A

A

S

A

P

S

K
|
K

P

P

A

A

P

P

D

D

T

I

F

F

L

I

R

A

C

A

A

A

E

H

L

A

M

V

G

G

I

V

A

A

P

P

E

S

R

E

C

S

I

I

V

G

F

L

E
|
E

D
|
D

A

S

K

Q

A

A

G

G

I

I

Q

Q

A

A

A

I

E

K

A

D

A

S

G

G

M

A

F

L

V

P

V

I

D

G

V

V

active site: D7 (= D11), L8 (≠ M12), N9 (≠ D13), T15 (≠ S19), K44 (≠ A47), S113 (≠ T111), A114 (≠ G112), K144 (= K144), E168 (= E168), D169 (= D169)
binding 1,6-di-O-phosphono-beta-D-glucopyranose: D7 (= D11), L8 (≠ M12), N9 (≠ D13), H19 (= H23), L43 (= L46), K44 (≠ A47), G45 (= G48), V46 (= V49), R48 (≠ T51), S113 (≠ T111), A114 (≠ G112), S115 (≠ T115), K116 (≠ E116), K144 (= K144)
binding magnesium ion: D7 (= D11), N9 (≠ D13), D169 (= D169)

Query Sequence

>7024893 FitnessBrowser__ANA3:7024893
MYTEFDGIIFDMDGTLVDSGQLHEQAWRQTLNHFGIPVEPALMRSLAGVPTIGTLEILIA
HFGVTPTASCEAMNEYKEALVRDTMHLYVKPTALAEFAKQNQGKRPMAVGTGAYTEEAIQ
ILTLCGLLELVDYVVGADQVASPKPAPDTFLRCAELMGIAPERCIVFEDAKAGIQAAEAA
GMFVVDVHAELQIENDYFLGTR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory