SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 7025106 Shewana3_2269 acetolactate synthase 3 catalytic subunit (RefSeq) to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P09114 Acetolactate synthase 2, chloroplastic; ALS II; Acetohydroxy-acid synthase II; Acetolactate synthase II; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see paper)
42% identity, 98% coverage: 2:564/572 of query aligns to 89:654/664 of P09114

query
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P09114

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P191 (= P104) mutation to A: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with L-568.
W568 (= W484) mutation to L: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with A-191.

P09342 Acetolactate synthase 1, chloroplastic; ALS I; Acetohydroxy-acid synthase I; Acetolactate synthase I; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see 2 papers)
41% identity, 98% coverage: 2:564/572 of query aligns to 92:657/667 of P09342

query
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P09342

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C161 (≠ V71) modified: Disulfide link with 307
P194 (= P104) mutation to Q: In C3; highly resistant to sulfonylurea herbicides.
C307 (≠ A217) modified: Disulfide link with 161

8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
41% identity, 98% coverage: 6:564/572 of query aligns to 14:575/582 of 8et4A

query
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8et4A

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-

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N

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binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V395), G401 (= G396), Q402 (= Q397), H403 (= H398), G426 (= G421), A427 (≠ T422), M428 (= M423), G452 (= G447), D453 (= D448), G454 (= G449), S455 (= S450), M458 (= M453), N480 (= N475), H482 (≠ F477), L483 (= L478), G484 (= G479), M485 (= M480), V486 (= V481)
binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G214), G223 (= G215), G224 (= G216), T246 (= T240), L247 (= L241), M248 (= M242), M263 (= M257), L264 (= L258), G265 (= G259), M266 (= M260), H267 (= H261), G286 (= G280), V287 (= V281), R288 (= R282), D290 (= D284), R292 (= R286), V293 (≠ T287), D310 (= D304), I311 (= I305), D312 (= D306), E315 (≠ S309), G328 (= G322), D329 (≠ S323), V330 (≠ A324), Q404 (= Q399), M405 (= M400), G423 (= G418), G424 (= G419)
binding magnesium ion: F370 (≠ Y365), Y381 (≠ Q376), D453 (= D448), M458 (= M453), Q461 (= Q456), N480 (= N475), H482 (≠ F477), K533 (≠ P521)
binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: M266 (= M260), H267 (= H261), R292 (= R286), M485 (= M480), V486 (= V481), W489 (= W484), S568 (≠ R557)

5wj1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a triazolopyrimidine herbicide, penoxsulam (see paper)
41% identity, 98% coverage: 6:564/572 of query aligns to 14:575/582 of 5wj1A

query
sites
5wj1A

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active site: Y33 (= Y25), G35 (= G27), G36 (= G28), A37 (≠ S29), S38 (≠ V30), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M260), V293 (≠ T287), V400 (= V395), G426 (= G421), M428 (= M423), D453 (= D448), N480 (= N475), H482 (≠ F477), L483 (= L478), M485 (= M480), V486 (= V481), W489 (= W484), H558 (≠ E547)
binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G214), G223 (= G215), G224 (= G216), T246 (= T240), L247 (= L241), M248 (= M242), M263 (= M257), L264 (= L258), G265 (= G259), M266 (= M260), H267 (= H261), G286 (= G280), V287 (= V281), R288 (= R282), D290 (= D284), R292 (= R286), V293 (≠ T287), I309 (= I303), D310 (= D304), I311 (= I305), D312 (= D306), E315 (≠ S309), G328 (= G322), D329 (≠ S323), V330 (≠ A324), Q404 (= Q399), M405 (= M400), G423 (= G418), G424 (= G419), M485 (= M480)
binding magnesium ion: D453 (= D448), N480 (= N475), H482 (≠ F477)
binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M266 (= M260), D291 (= D285), R292 (= R286), M485 (= M480), V486 (= V481), W489 (= W484), S568 (≠ R557), G569 (= G558)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V395), G401 (= G396), Q402 (= Q397), H403 (= H398), G426 (= G421), A427 (≠ T422), M428 (= M423), G452 (= G447), D453 (= D448), G454 (= G449), S455 (= S450), M458 (= M453), N480 (= N475), H482 (≠ F477), L483 (= L478), G484 (= G479), M485 (= M480), V486 (= V481)

5k6tA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, propoxycarbazone-sodium (see paper)
41% identity, 98% coverage: 6:564/572 of query aligns to 14:575/582 of 5k6tA

query
sites
5k6tA

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active site: Y33 (= Y25), G35 (= G27), G36 (= G28), A37 (≠ S29), S38 (≠ V30), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M260), V293 (≠ T287), V400 (= V395), G426 (= G421), M428 (= M423), D453 (= D448), N480 (= N475), H482 (≠ F477), L483 (= L478), M485 (= M480), V486 (= V481), W489 (= W484), H558 (≠ E547)
binding methyl 2-[(4-methyl-5-oxidanylidene-3-propoxy-1,2,4-triazol-1-yl)carbonylsulfamoyl]benzoate: H267 (= H261), D291 (= D285), R292 (= R286), M485 (= M480), W489 (= W484), S568 (≠ R557)
binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G214), G223 (= G215), G224 (= G216), T246 (= T240), L247 (= L241), M248 (= M242), M263 (= M257), L264 (= L258), G265 (= G259), M266 (= M260), H267 (= H261), G286 (= G280), V287 (= V281), R288 (= R282), D290 (= D284), R292 (= R286), V293 (≠ T287), I309 (= I303), D310 (= D304), I311 (= I305), D312 (= D306), E315 (≠ S309), G328 (= G322), D329 (≠ S323), V330 (≠ A324), Q404 (= Q399), M405 (= M400), G423 (= G418), G424 (= G419)
binding magnesium ion: D453 (= D448), N480 (= N475), H482 (≠ F477)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (= V395), G401 (= G396), Q402 (= Q397), H403 (= H398), G426 (= G421), M428 (= M423), G452 (= G447), D453 (= D448), G454 (= G449), S455 (= S450), M458 (= M453), N480 (= N475), H482 (≠ F477), L483 (= L478), G484 (= G479), M485 (= M480), V486 (= V481)

5k6rA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, thiencarbazone-methyl (see paper)
41% identity, 98% coverage: 6:564/572 of query aligns to 14:575/582 of 5k6rA

query
sites
5k6rA

G

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A

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active site: Y33 (= Y25), G35 (= G27), G36 (= G28), A37 (≠ S29), S38 (≠ V30), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M260), V293 (≠ T287), V400 (= V395), G426 (= G421), M428 (= M423), D453 (= D448), N480 (= N475), H482 (≠ F477), L483 (= L478), M485 (= M480), V486 (= V481), W489 (= W484), H558 (≠ E547)
binding methyl 4-[(3-methoxy-4-methyl-5-oxidanylidene-1,2,4-triazol-1-yl)carbonylsulfamoyl]-5-methyl-thiophene-3-carboxylate: D291 (= D285), R292 (= R286), W489 (= W484), S568 (≠ R557)
binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G214), G223 (= G215), G224 (= G216), T246 (= T240), L247 (= L241), M248 (= M242), M263 (= M257), L264 (= L258), G265 (= G259), M266 (= M260), H267 (= H261), G286 (= G280), V287 (= V281), R288 (= R282), D290 (= D284), R292 (= R286), V293 (≠ T287), I309 (= I303), D310 (= D304), I311 (= I305), D312 (= D306), E315 (≠ S309), G328 (= G322), D329 (≠ S323), V330 (≠ A324), Q404 (= Q399), M405 (= M400), G423 (= G418), G424 (= G419)
binding magnesium ion: D453 (= D448), N480 (= N475), H482 (≠ F477)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (= V395), G401 (= G396), Q402 (= Q397), H403 (= H398), G426 (= G421), M428 (= M423), G452 (= G447), D453 (= D448), G454 (= G449), S455 (= S450), M458 (= M453), N480 (= N475), H482 (≠ F477), L483 (= L478), G484 (= G479), M485 (= M480), V486 (= V481)

1z8nA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with an imidazolinone herbicide, imazaquin (see paper)
41% identity, 98% coverage: 6:564/572 of query aligns to 14:575/582 of 1z8nA

query
sites
1z8nA

G

G

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G

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