SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 7025568 FitnessBrowser__ANA3:7025568 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 9 hits to proteins with known functional sites (download)

P54582 Glycine betaine transporter BetP; Glycine betaine permease from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) (see 6 papers)
38% identity, 70% coverage: 34:496/658 of query aligns to 85:547/595 of P54582

query
sites
P54582

N

T

H

N

F

F

F

A

K

S

S

S

M

A

Q

L

S

S

W

A

L

V

E

V

A

D

K

N

A

L

G

G

W
|
W

L

A

Y

F

I

I

L

L

G

F

V

G

A

T

I

V

F

F

L

V

I

F

F

F

I

I

I

V

F

V

V

I

M

A

V

A

S

S

R

K

F

F

G

G

D

T

I

I

K

R

L

L

G

G

P

R

D

I

H

D

A

E

V

A

P

P

D
x
E

Y

F

S

R

Y

T

K

V

S

S

W

W

I

I

A

S

M

M

L

M

F

F

S

A

A

A

G
|
G

M
|
M

G
|
G

I
|
I

G
|
G

L

L

M

M

F
|
F

F

Y

G

G

V

T

A

T

E

E

P

P

V

L

M

T

H

F

Y

Y

L

R

A

N

P

G

P

V

D

P

A

G

T

H

P

D

E

E

S

H

L

N

A

V

A

G

A

V

K

-

E

-

A

A

M

M

K

S

I

T

T

T

F

M

F

F

H

H

W
|
W

G

T

I

L

H

H

A

P

W
|
W

A

A

I

I

Y
|
Y

G

A

V

I

V

V

A

G

L

L

S

A

L

I

A

A

Y

Y

F

S

A

T

Y

F

R
|
R

H

V

K

G

L

R

P

K

L

Q

L

L

P

L

R

S

S

S

A

A

L

F

Y

V

P

P

L

L

I

I

G

G

E

E

R

K

-

G

I

A

H

E

G

G

P

W

I

L

G

G

H

K

C

L

V

I

D

D

T

I

F

L

A

A

V

I

L

I

G

A

T

T

M

V

F

F

G

G

V

T

A

A

T

C

S
|
S

L

L

G

G

F

L

G

G

V

A

L

L

Q

Q

V

I

N

G

S

A

G

G

L

L

S

S

Y

A

-

A

-

N

L

I

F

I

E

E

Q

D

L

-

P

P

N

S

N

D

T

W

T

T

V

I

Q

-

V

V

S

G

L

I

I

V

I

S

G

V

I

L

T

T

L

L

L

A

A

F

T

I

L

F

S

S

V

A

F

I

S

S

G

G

L

V

D

G

K

K

G
|
G

V

I

K

Q

R

Y

L

L

S

S

E

N

L

A

N
|
N

L

M

G

V

L

L

A

A

L

A

I

L

L

L

L

A

L

I

I

F

V

V

L

F

I

V

L

V

G

G

P

P

T

T

V

V

M

S

L

I

L

L

Q

N

A

L

F

L

V

P

Q

G

N

S

T

I

G

G

S

N

Y

Y

L

L

S

S

D

N

-

F

-

Q

I

M

V

A

N

G

K

R

T
|
T

F

-

N

A

L

M

Y

S

A

A

Y

D

Q

G

H

T

K

A

E

G

D

E

W
|
W

L

L

G

G

G

S

W
|
W

T

T

L

I

L
x
F

Y

Y

W
|
W

G

A

W
|
W

I
|
I

S
|
S

W
|
W

S

S

P

P

F
|
F

V

V

G

G

T

M

F
|
F

I

L

A

A

R
|
R

V

I

S

S

R

R

G

G

R
|
R

T

S

I

I

R

R

E

E

F

F

L

I

V

L

G

G

I

V

L

L

F

L

V

V

P

P

S

A

A

G

L

V

T

S

F

T

L

V

W

W

M

F

T

S

V

I

F

F

G

G

N

G

S

T

A

A

I

I

D

V

A

F

I

E

M

Q

N

N

Q

G

G

E

A

S

T

I

Y

W

L

G

S

D

E

G

A

A

V

A

N

E

T

E

N

Q

V

-

P

-

V

-

A

-

L

L

F

F

V

G

F

L

E

H

H

A

M

L

P

P

F

G

P

G

H

Q

L

I

L

M

S

G

G

I

I

I

G

A

I

M

C

I

L

L

V

L

V

G

T

T

F

F

V

I

T

T

S

S

S

A

D

D

S

S

G

A

S

S

L

T

V

V

I

M

D

G

N

T

L

M

T

S

S

Q

G

H

G

G

D

Q

N

L

N

E

A

A

P

N

V

K

W

W

Q

V

R

T

V

A

F

A

W

W

A

G

L

V

L

A

Q

T

G

A

V

A

V

I

A

G

S

L

V

T

L

L

A

S

G

G

-

D

-

N

-

A

L

L

Q

S

A

N

L

L

Q

Q

T

N

A

V

A

T

I

I

A

V

S

A

A

A

M

T

P

P

F

F

L

L

C

F

V

V

M

V

L

I

L

G

M

L

C

M

L

F

G

A

L

L

Y

V

K

K

A

D

L

L

K

S

D

N

D

D

W101 (= W50) mutation to A: Mainly monomeric, shows a decrease in activity and cannot be activated in response to increased osmolality; when associated with A-351.
E135 (≠ D84) mutation to A: Strongly decreased betaine transport.
G149 (= G98) mutation to A: Decreases betaine transport. No effect on activation by increased osmolality.
M150 (= M99) mutation to F: No effect on activation by increased osmolality; when associated with A-152.
G151 (= G100) mutation to A: Nearly abolishes betaine transport.
I152 (= I101) mutation to A: No effect on activation by increased osmolality; when associated with F-150.
IG 152:153 (= IG 101:102) binding
G153 (= G102) mutation to A: Decreases betaine transport and alters activation at higher osmolality.; mutation to D: Changes substrate specificity, giving rise to proton-coupled choline transport. Decreases sodium-dependent betaine transport.
F156 (= F105) mutation to A: Decreases betaine transport, but has no major effect on affinity for glycine betaine.
W189 (= W140) mutation to C: Mildly decreased betaine transport.
W194 (= W145) mutation to L: Strongly decreased betaine transport.
Y197 (= Y148) mutation to L: Nearly abolishes betaine transport.
R210 (= R161) mutation to A: Nearly abolishes betaine transport.
S253 (= S203) binding
G301 (= G251) mutation to L: Strongly decreased betaine transport.
N309 (= N259) mutation to A: Decreases affinity for sodium ions.
T351 (= T298) mutation to A: Mainly trimeric, but shows reduced activity at high osmolalities. Mainly monomeric, shows a decrease in activity and cannot be activated in response to increased osmolality; when associated with A-101.
W362 (= W310) mutation to C: Strongly decreased betaine transport.
W366 (= W314) mutation to C: No effect on betaine transport.
F369 (≠ L317) mutation to G: Decreases affinity for glycine betaine. Decreases betaine transport.
W371 (= W319) mutation to L: No effect on betaine transport.
W373 (= W321) mutation to A: Strongly decreases affinity for glycine betaine and betaine transport.
WWISW 373:377 (= WWISW 321:325) binding
W374 (= W322) mutation to A: Strongly decreases betaine transport, but has no major effect on affinity for glycine betaine.; mutation to L: No effect on betaine transport.
W377 (= W325) mutation to A: Abolishes betaine transport.; mutation to L: Nearly abolishes betaine transport.
F380 (= F328) mutation to A: Decreases betaine transport, but has no effect on affinity for glycine betaine.
F384 (= F332) mutation to A: Decreases betaine transport, but has no effect on affinity for glycine betaine.
R387 (= R335) mutation to A: Mildly decreased betaine transport.
R392 (= R340) mutation to K: Moderately decreased betaine transport.

4llhA Substrate bound outward-open state of the symporter betp (see paper)
38% identity, 70% coverage: 34:496/658 of query aligns to 29:488/524 of 4llhA

query
sites
4llhA

N

T

H

N

F

F

F

A

K

S

S

S

M

A

Q

L

S

S

W

A

L

V

E

V

A

D

K

N

A

L

G

G

W

W

L

A

Y

F

I

I

L

L

G

F

V

G

A

T

I

V

F

F

L

V

I

F

F

F

I

I

I

V

F

V

V

I

M

A

V

A

S

S

R

K

F

F

G

G

D

T

I

I

K

R

L

L

G

G

P

R

D

I

H

D

A

E

V

A

P

P

D

E

Y

F

S

R

Y

T

K

V

S

S

W

W

I

I

A

S

M

M

L

M

F

F

S
x
A

A

A

G

G

M
|
M

G
|
G

I

I

G
x
D

L

L

M

M

F

F

F

Y

G

G

V

T

A

T

E

E

P

P

V

L

M

T

H

F

Y

Y

L

R

A

N

P

G

P

V

D

P

A

G

T

H

P

D

E

E

S

H

L

N

A

V

A

G

A

V

K

-

E

-

A

A

M

M

K

S

I

T

T

T

F

M

F

F

H

H

W

W

G

T

I

L

H

H

A

P

W

W

A

A

I

I

Y

Y

G

A

V

I

V

V

A

G

L

L

S

A

L

I

A

A

Y

Y

F

S

A

T

Y

F

R

R

H

V

K

G

L

R

P

K

L

Q

L

L

P

L

R

S

S

S

A

A

L

F

Y

V

P

P

L

L

I

I

G

G

E

E

R

K

-

G

I

A

H

E

G

G

P

W

I

L

G

G

H

K

C

L

V

I

D

D

T

I

F

L

A

A

V

I

L

I

G

A

T

T

M

V

F

F

G

G

V

T

A

A

T

C

S

S

L

L

G

G

F

L

G

G

V

A

L

L

Q

Q

V

I

N

G

S

A

G

G

L

L

S

S

Y

A

L

-

F

-

E

A

Q

E

L

D

P

P

N

S

N

D

T

W

T

T

V

I

Q

-

V

V

S

G

L

I

I

V

I

S

G

V

I

L

T

T

L

L

L

A

A

F

T

I

L

F

S

S

V

A

F

I

S

S

G

G

L

V

D

G

K

K

G

G

V

I

K

Q

R

Y

L

L

S

S

E

N

L

A

N

N

L

M

G

V

L

L

A

A

L

A

I

L

L

L

L

A

L

I

I

F

V

V

L

F

I

V

L

V

G

G

P

P

T

T

V

V

M

S

L

I

L

L

Q

N

A

L

F

L

V

P

Q

G

N

S

T

I

G

G

S

N

Y

Y

L

L

S

S

D

N

-

F

-

Q

I

M

V

A

N

G

K

R

T

T

F

-

N

A

L

M

Y

S

A

A

Y

D

Q

G

H

T

K

A

E

G

D

E

W

W

L

L

G

G

G

S

W

W

T

T

L

I

L

F

Y

Y

W

W

G

A

W
|
W

I

I

S

S

W
|
W

S

S

P

P

F

F

V

V

G

G

T

M

F

F

I

L

A

A

R

R

V

I

S

S

R

R

G

G

R

R

T

S

I

I

R

R

E

E

F

F

L

I

V

L

G

G

I

V

L

L

F

L

V

V

P

P

S

A

A

G

L

V

T

S

F

T

L

V

W

W

M

F

T

S

V

I

F

F

G

G

N

G

S

T

A

A

I

I

D

V

A

F

I

E

M

Q

N

N

Q

G

G

E

A

S

T

I

Y

W

L

G

S

D

E

G

A

A

V

A

N

E

T

E

N

Q

V

-

P

-

V

-

A

-

L

L

F

F

V

G

F

L

E

H

H

A

M

L

P

P

F

G

P

G

H

Q

L

I

L

M

S

G

G

I

I

I

G

A

I

M

C

I

L

L

V

L

V

G

T

T

F
|
F

F

F

V

I

T
|
T

S
|
S

S

A

D

D

S

S

G

A

S

S

L

T

V

V

I

M

D

G

N

T

L

M

T

S

S

Q

G

H

G

G

D

Q

N

L

N

E

A

A

P

N

V

K

W

W

Q

V

R

T

V

A

F

A

W

W

A

G

L

V

L

A

Q

T

G

A

V

A

V

I

A

G

S

L

V

T

L

L

A

S

G

G

-

D

-

N

-

A

L

L

Q

S

A

N

L

L

Q

Q

T

N

A

V

A

T

I

I

A

V

S

A

A

A

M

T

P

P

F

F

L

L

C

F

V

V

M

V

L

I

L

G

M

L

C

M

L

F

G

A

L

L

Y

V

K

K

A

D

L

L

K

S

D

N

D

D

binding 2-(trimethyl-lambda~5~-arsanyl)ethanol: M94 (= M99), G95 (= G100), D97 (≠ G102), W314 (= W321), W315 (= W322), W318 (= W325)
binding sodium ion: A91 (≠ S96), M94 (= M99), G95 (= G100), F405 (= F416), T408 (= T419), S409 (= S420)

Sites not aligning to the query:

binding 5-cyclohexyl-1-pentyl-beta-d-maltoside: 495, 499

3p03C Crystal structure of betp-g153d with choline bound (see paper)
38% identity, 70% coverage: 34:496/658 of query aligns to 29:487/508 of 3p03C

query
sites
3p03C

N

T

H

N

F

F

F

A

K

S

S

S

M

A

Q

L

S

S

W

A

L

V

E

V

A

D

K

N

A

L

G

G

W

W

L

A

Y

F

I

I

L

L

G

F

V

G

A

T

I

V

F

F

L

V

I

F

F

F

I

I

I

V

F

V

V

I

M

A

V

A

S

S

R

K

F

F

G

G

D

T

I

I

K

R

L

L

G

G

P

R

D

I

H

D

A

E

V

A

P

P

D

E

Y

F

S

R

Y

T

K

V

S

S

W

W

I

I

A

S

M

M

L

M

F

F

S

A

A
|
A

G

G

M
|
M

G

G

I

I

G
x
D

L

L

M

M

F

F

F

Y

G

G

V

T

A

T

E

E

P

P

V

L

M

T

H

F

Y

Y

L

R

A

N

P

G

P

V

D

P

A

G

T

H

P

D

E

E

S

H

L

N

A

V

A

G

A

V

K

-

E

-

A

A

M

M

K

S

I

T

T

T

F

M

F

F

H

H

W

W

G

T

I

L

H

H

A

P

W

W

A

A

I

I

Y

Y

G

A

V

I

V

V

A

G

L

L

S

A

L

I

A

A

Y

Y

F

S

A

T

Y

F

R

R

H

V

K

G

L

R

P

K

L

Q

L

L

P

L

R

S

S

S

A

A

L

F

Y

V

P

P

L

L

I

I

G

G

E

E

R

K

-

G

I

A

H

E

G

G

P

W

I

L

G

G

H

K

C

L

V

I

D

D

T

I

F

L

A

A

V

I

L

I

G

A

T

T

M

V

F

F

G

G

V

T

A

A

T

C

S

S

L

L

G

G

F

L

G

G

V

A

L

L

Q

Q

V

I

N

G

S

A

G

G

L

L

S

S

Y

A

L

-

F

-

E

A

Q

N

L

I

P

I

N

D

N

W

T

T

T

I

V

V

Q

G

V

I

S

V

L

S

I

V

I

L

G

-

I

-

T

T

L

L

L

A

A

F

T

I

L

F

S

S

V

A

F

I

S

S

G

G

L

V

D

G

K

K

G

G

V

I

K

Q

R

Y

L

L

S

S

E

N

L

A

N

N

L

M

G

V

L

L

A

A

L

A

I

L

L

L

L

A

L

I

I

F

V

V

L

F

I

V

L

V

G

G

P

P

T

T

V

V

M

S

L

I

L

L

Q

N

A

L

F

L

V

P

Q

G

N

S

T

I

G

G

S

N

Y

Y

L

L

S

S

D

N

-

F

-

Q

I

M

V

A

N

G

K

R

T

T

F

-

N

A

L

M

Y

S

A

A

Y

D

Q

G

H

T

K

A

E

G

D

E

W

W

L

L

G

G

G

S

W

W

T

T

L

I

L

F

Y

Y

W

W

G

A

W

W

I

I

S

S

W

W

S

S

P

P

F
|
F

V

V

G

G

T

M

F

F

I

L

A

A

R

R

V

I

S

S

R

R

G

G

R

R

T

S

I

I

R

R

E

E

F

F

L

I

V

L

G

G

I

V

L

L

F

L

V

V

P

P

S

A

A

G

L

V

T

S

F

T

L

V

W

W

M

F

T

S

V

I

F

F

G

G

N

G

S

T

A

A

I

I

D

V

A

F

I

E

M

Q

N

N

Q

G

G

E

A

S

T

I

Y

W

L

G

S

D

E

G

A

A

V

A

N

E

T

E

N

Q

V

-

P

-

V

-

A

-

L

L

F

F

V

G

F

L

E

H

H

A

M

L

P

P

F

G

P

G

H

Q

L

I

L

M

S

G

G

I

I

I

G

A

I

M

C

I

L

L

V

L

V

G

T

T

F

F

V

I

T

T

S

S

S

A

D

D

S

S

G

A

S

S

L

T

V

V

I

M

D

G

N

T

L

M

T

S

S

Q

G

H

G

G

D

Q

N

L

N

E

A

A

P

N

V

K

W

W

Q

V

R

T

V

A

F

A

W

W

A

G

L

V

L

A

Q

T

G

A

V

A

V

I

A

G

S

L

V

T

L

L

A

S

G

G

-

D

-

N

-

A

L

L

Q

S

A

N

L

L

Q

Q

T

N

A

V

A

T

I

I

A

V

S

A

A

A

M

T

P

P

F

F

L

L

C

F

V

V

M

V

L

I

L

G

M

L

C

M

L

F

G

A

L

L

Y

V

K

K

A

D

L

L

K

S

D

N

D

D

binding choline ion: A92 (= A97), M94 (= M99), D97 (≠ G102), F320 (= F328)

2wswA Crystal structure of carnitine transporter from proteus mirabilis (see paper)
27% identity, 75% coverage: 4:497/658 of query aligns to 12:507/508 of 2wswA

query
sites
2wswA

K

K

S

A

S

G

I

I

N

E

P

P

P

K

V

V

F

F

Y

F

S

P

V

L

F

I

I

V

T

G

L

I

M

L

V

C

M

W

I

L

C

T

A

V

I

R

W

D

P

L

T

D

E

A

A

S

N

N

H

E

F

V

F

I

K

N

S

A

M

V

Q

F

S

S

W

Y

L

V

E

T

A

N

K

V

A

W

G

G

W

W

L

A

Y

F

I

E

L

W

G

Y

V

M

A

V

I

I

F

M

L

F

I

G

F

G

I

W

I
x
F

F

W

V

L

M

V

V
x
F

S

G

R
|
R

F

Y

G

A

D

K

I

K

K

R

L

L

G

G

P

D

D

E

H

K

A

-

V

-

P

P

D

E

Y

F

S

S

Y

T

K

A

S

S

W

W

I

I

A

F

M

M

L

M

F

F

S

A

A

S

G

C

M

T

G

S

I

A

G

A

L

V

M

L

F

F

F

W

G

G

V

S

A

I

E

E

P

I

V

Y

M

Y

H

Y

Y

I

L

S

A

S

P

P

D

F

A

G

T

M

P

E

E

G

S

Y

L

S

A

A

A

P

A

A

K

K

E

E

-

I

A

G

M

L

K

A

I

Y

T

S

F

L

F

F

H

H

W

W

G

G

I

P

H

L

A

P

W

W

A

A

I

T

Y

Y

G

S

V

F

V

L

A

S

L

V

S

A

L

F

A

A

Y

Y

F

F

A

F

Y

F

R

V

H

R

K

K

L

M

P

E

L

V

L

I

-

R

P

P

R

S

S

S

A

T

L

L

Y

V

P

P

L

L

I

V

G

G

E

E

R

K

-

H

I

V

H

N

G

G

P

L

I

F

G

G

H

T

C

V

V

V

D

D

T

N

F

F

A

Y

V

L

L

V

G

A

T

L

M

I

F

L

G

A

V

M

A

G

T

T

S

S

L

L

G

G

F

L

G

A

V

T

L

P

Q

L

V

V

N

T

S

E

G

C

L

I

S

Q

Y

Y

L

L

F

F

E

-

Q

G

L

I

P

P

N

H

N

T

T

L

T

Q

V

L

Q

D

V

A

S

I

L

I

I

I

I

S

G

C

I

W

T

I

L

L

A

N

T

A

L

I

S

C

V

V

F

A

S

F

G

G

L

L

D

Q

K

K

G

G

V

V

K

K

R

I

L

A

S

S

E

D

L

V

N

R

L

T

G

Y

L

L

A

S

L

F

I

L

L

M

L

L

L

G

I

W

V

V

L

F

I

I

L

V

G

G

P

G

T

A

V

S

M

F

L

I

L

V

Q

N

A

Y

F

F

V

T

Q

D

N

S

T

V

G

G

S

T

Y

L

L

L

S

M

D

Y

I

M

V

P

N

R

K

M

T

L

F

F

N

Y

L

T

Y

D

A

P

Y

I

Q

-

H

G

K

K

E

G

D

G

W

F

L

P

G

Q

G

A

W

W

T

T

L

V

L

F

Y

Y

W

W

G

A

W

W

I

V

S

I

W

Y

S

A

P

I

F

Q

V

M

G

S

T

I

F

F

I

L

A

A

R

R

V

I

S

S

R

K

G

G

R

R

T

T

I

V

R

R

E

E

F

L

L

C

V

L

G

G

I

M

L

V

F

S

V

G

P

L

S

T

A

A

L

G

T

T

F

W

L

L

W

I

M

W

T

T

V

I

F

L

G

G

N

G

S

N

A

T

I

L

D

Q

A

L

I

I

M

-

N

D

Q

Q

G

N

A

I

T

L

Y

N

L

I

S

P

E

Q

A

L

V

I

N

D

T

Q

-

Y

N

G

V

V

P

P

V

R

A

A

L

I

F

I

V

E

F

T

F

W

E

A

H

A

M

L

P

P

F

L

-

S

-

T

P

A

H

T

L

M

L

W

S

G

G

F

I

F

G

I

I

L

C

C

L

F

V

I

V

A

T

T

F

V

F

T

V

L

T

I

S

N

S

A

D

C

S

S

G

Y

S

T

L

L

V

A

I

M

D

S

N

T

L

C

T

R

S

S

G

M

G

K

D

E

N

G

-

A

N

D

A

P

P

P

V

L

W

L

Q

V

R

R

V

I

F

G

W

W

A

S

L

V

L

L

Q

V

G

G

V

I

A

G

S

I

V

I

L

L

L

A

A

L

G

G

L

L

Q

K

A

P

L

I

Q

Q

T

T

A

A

A

I

I

I

A

A

S

G

A

G

M

C

P

P

F

L

L

F

C

F

V

V

M

N

L

I

L

M

M

V

C

T

L

L

G

S

L

F

Y

I

K

K

A

D

L

A

K

K

D

V

D

H

W

W

binding 5-cyclohexyl-1-pentyl-beta-d-maltoside: F72 (≠ I64), F76 (≠ V68), R78 (= R70)

B4EY22 L-carnitine/gamma-butyrobetaine antiporter from Proteus mirabilis (strain HI4320) (see 2 papers)
27% identity, 75% coverage: 4:497/658 of query aligns to 7:502/514 of B4EY22

query
sites
B4EY22

K

K

S

A

S

G

I

I

N

E

P

P

P

K

V

V

F

F

Y

F

S

P

V

L

F

I

I

V

T

G

L

I

M

L

V

C

M

W

I

L

C

T

A

V

I

R

W

D

P

L

T

D

E

A

A

S

N

N

H

E

F

V

F

I

K

N

S

A

M

V

Q

F

S

S

W

Y

L

V

E

T

A

N

K

V

A

W

G

G

W

W

L

A

Y

F

I

E

L

W

G

Y

V

M

A

V

I

I

F

M

L

F

I

G

F

G

I

W

I

F

F

W

V

L

M

V

V

F

S

G

R

R

F

Y

G

A

D

K

I

K

K

R

L

L

G

G

P

D

D

E

H

K

A

-

V

-

P

P

D

E

Y

F

S

S

Y

T

K

A

S

S

W

W

I

I

A

F

M

M

L

M

F

F

S

A

A

S

G

C

M

T

G

S

I

A

G

A

L

V

M

L

F

F

F

W

G

G

V

S

A

I

E
|
E

P

I

V

Y

M

Y

H

Y

Y

I

L

S

A

S

P

P

D

F

A

G

T

M

P

E

E

G

S

Y

L

S

A

A

A

P

A

A

K

K

E

E

-

I

A

G

M

L

K

A

I

Y

T

S

F

L

F

F

H

H

W

W

G

G

I

P

H

L

A

P

W

W

A

A

I

T

Y

Y

G

S

V

F

V

L

A

S

L

V

S

A

L

F

A

A

Y

Y

F

F

A

F

Y

F

R

V

H

R

K

K

L

M

P

E

L

V

L

I

-

R

P

P

R

S

S

S

A

T

L

L

Y

T

P

P

L

L

I

V

G

G

E

E

R

K

-

H

I

V

H

N

G

G

P

L

I

F

G

G

H

T

C

V

V

V

D

D

T

N

F

F

A

Y

V

L

L

V

G

A

T

L

M

I

F

L

G

A

V

M

A

G

T

T

S

S

L

L

G

G

F

L

G

A

V

T

L

P

Q

L

V

V

N

T

S

E

G

C

L

I

S

Q

Y

Y

L

L

F

F

E

-

Q

G

L

I

P

P

N

H

N

T

T

L

T

Q

V

L

Q

D

V

A

S

I

L

I

I

I

I

S

G

C

I

W

T

I

L

L

A

N

T

A

L

I

S

C

V

V

F

A

S

F

G

G

L

L

D

Q

K

K

G

G

V

V

K

K

R

I

L

A

S

S

E

D

L

V

N
x
R

L

T

G

Y

L

L

A

S

L

F

I

L

L

M

L

L

L

G

I

W

V

V

L

F

I

I

L

V

G

G

P

G

T

A

V

S

M

F

L

I

L

V

Q

N

A

Y

F

F

V

T

Q

D

N

S

T

V

G

G

S

T

Y

L

L

L

S

M

D

Y

I

M

V

P

N

R

K

M

T

L

F

F

N

Y

L

T

Y

D

A

P

Y

I

Q

-

H

G

K

K

E

G

D

G

W

F

L

P

G

Q

G

A

W
|
W

T

T

L

V

L

F

Y

Y

W

W

G

A

W

W

I

V

S

I

W

Y

S

A

P

I

F

Q

V
x
M

G

S

T

I

F

F

I

L

A

A

R

R

V

I

S

S

R

K

G

G

R

R

T

T

I

V

R

R

E

E

F

L

L

C

V

L

G

G

I

M

L

V

F

S

V

G

P

L

S

T

A

A

L

G

T

T

F

W

L

L

W

I

M

W

T

T

V

I

F

L

G

G

N

G

S

N

A

T

I

L

D

Q

A

L

I

I

M

-

N

D

Q

Q

G

N

A

I

T

L

Y

N

L

I

S

P

E

Q

A

L

V

I

N

D

T

Q

-

Y

N

G

V

V

P

P

V

R

A

A

L

I

F

I

V

E

F

T

F

W

E

A

H

A

M

L

P

P

F

L

P

S

-

T

-

A

H

T

L

M

L

W

S

G

G

F

I

F

G

I

I

L

C

C

L

F

V

I

V

A

T

T

F

V

F

T

V

L

T

I

S

N

S

A

D

C

S

S

G

Y

S

T

L

L

V

A

I

M

D

S

N

T

L

C

T

R

S

S

G

M

G

K

D

E

N

G

-

A

N

E

A

P

P

P

V

L

W

L

Q

V

R

R

V

I

F

G

W

W

A

S

L

V

L

L

Q

V

G

G

V

I

A

G

S

I

V

I

L

L

L

A

A

L

G

G

L

L

Q

K

A

P

L

I

Q

Q

T

T

A

A

A

I

I

I

A

A

S

G

A

G

M

C

P

P

F

L

L

F

C

F

V

V

M

N

L

I

L

M

M

V

C

T

L

L

G

S

L

F

Y

I

K

K

A

D

L

A

K

K

D

V

D

H

W

W

E111 (= E110) mutation to A: Abolishes transport activity.
R262 (≠ N259) mutation R->A,E: Strong decrease in L-carnitine transport. Mutant is Na(+)-dependent for substrate binding and transport.
W316 (= W314) mutation to A: 2.5-fold decrease in Vmax.
M331 (≠ V329) mutation to V: 10-fold decrease in Vmax.

4m8jA Crystal structure of cait r262e bound to gamma-butyrobetaine (see paper)
27% identity, 75% coverage: 7:497/658 of query aligns to 2:494/495 of 4m8jA

query
sites
4m8jA

I

I

N

E

P

P

P

K

V

V

F

F

Y

F

S

P

V

L

F

I

I

V

T

G

L

I

M

L

V

C

M

W

I

L

C

T

A

V

I

R

W

D

P

L

T

D

E

A

A

S

N

N

H

E

F

V

F

I

K

N

S

A

M

V

Q

F

S

S

W

Y

L

V

E

T

A

N

K

V

A

W

G

G

W

W

L

A

Y

F

I

E

L

W

G

Y

V

M

A

V

I

I

F

M

L

F

I

G

F

G

I

W

I

F

F

W

V

L

M

V

V

F

S

G

R

R

F

Y

G

A

D

K

I

K

K

R

L

L

G

G

P

D

D

E

H

K

A

-

V

-

P

P

D

E

Y

F

S

S

Y

T

K

A

S

S

W

W

I

I

A

F

M

M

L

M

F

F

S

A

A

S

G

C

M

T

G

S

I

A

G

A

L

V

M

L

F

F

F

W

G

G

V

S

A

I

E

E

P

I

V

Y

M

Y

H

Y

Y

I

L

S

A

S

P

P

D

F

A

G

T

M

P

E

E

G

S

Y

L

S

A

A

A

P

A

A

K

K

E

E

-

I

A

G

M

L

K

A

I

Y

T

S

F

L

F

F

H

H

W

W

G

G

I

P

H

L

A

P

W
|
W

A

A

I

T

Y

Y

G

S

V

F

V

L

A

S

L

V

S

A

L

F

A

A

Y

Y

F

F

A

F

Y

F

R

V

H

R

K

K

L

M

P

E

L

V

L

I

-

R

P

P

R

S

S

S

A

T

L

L

Y

V

P

P

L

L

I

V

G

G

E

E

R

K

-

H

I

V

H

N

G

G

P

L

I

F

G

G

H

T

C

V

V

V

D

D

T

N

F

F

A

Y

V

L

L

V

G

A

T

L

M

I

F

L

G

A

V

M

A

G

T

T

S

S

L

L

G

G

F

L

G

A

V

T

L

P

Q

L

V

V

N

T

S

E

G

C

L

I

S

Q

Y

Y

L

L

F

F

E

-

Q

G

L

I

P

P

N

H

N

T

T

L

T

Q

V

L

Q

D

V

A

S

I

L

I

I

I

I

S

G

C

I

W

T

I

L

L

A

N

T

A

L

I

S

C

V

V

F

A

S

F

G

G

L

L

D

Q

K

K

G

G

V

V

K

K

R

I

L

A

S

S

E

D

L

V

N

E

L

T

G

Y

L

L

A

S

L

F

I

L

L

M

L

L

L

G

I

W

V

V

L

F

I

I

L

V

G

G

P

G

T

A

V

S

M

F

L

I

L

V

Q

N

A

Y

F

F

V

T

Q

D

N

S

T

V

G

G

S

T

Y

L

L

L

S

M

D

Y

I

M

V

P

N

R

K

M

T

L

F

F

N

Y

L

T

Y

D

A

P

Y

I

Q

-

H

G

K

K

E

G

D

G

W

F

L

P

G

Q

G

A

W

W

T

T

L

V

L

F

Y

Y

W

W

G

A

W
|
W

I

V

S

I

W

Y

S

A

P

I

F

Q

V

M

G

S

T

I

F

F

I

L

A

A

R

R

V

I

S

S

R

K

G

G

R

R

T

T

I

V

R

R

E

E

F

L

L

C

V

L

G

G

I

M

L

V

F

S

V

G

P

L

S

T

A

A

L

G

T

T

F

W

L

L

W

I

M

W

T

T

V

I

F

L

G

G

N

G

S

N

A

T

I

L

D

Q

A

L

I

I

M

-

N

D

Q

Q

G

N

A

I

T

L

Y

N

L

I

S

P

E

Q

A

L

V

I

N

D

T

Q

-

Y

N

G

V

V

P

P

V

R

A

A

L

I

F

I

V

E

F

T

F

W

E

A

H

A

M

L

P

P

F

L

-

S

-

T

P

A

H

T

L

M

L

W

S

G

G

F

I

F

G

I

I

L

C

C

L

F

V

I

V

A

T

T

F

V

F

T

V

L

T

I

S

N

S

A

D

C

S

S

G

Y

S

T

L

L

V

A

I

M

D

S

N

T

L

C

T

R

S

S

G

M

G

K

D

E

N

G

-

A

N

D

A

P

P

P

V

L

W

L

Q

V

R

R

V

I

F

G

W

W

A

S

L

V

L

L

Q

V

G

G

V

I

A

G

S

I

V

I

L

L

L

A

A

L

G

G

L

L

Q

K

A

P

L

I

Q

Q

T

T

A

A

A

I

I

I

A

A

S

G

A

G

M

C

P

P

F

L

L

F

C

F

V

V

M

N

L

I

L

M

M

V

C

T

L

L

G

S

L

F

Y

I

K

K

A

D

L

A

K

K

D

V

D

H

W

W

binding 3-carboxy-n,n,n-trimethylpropan-1-aminium: W139 (= W145), W315 (= W321), W316 (= W322)

P31553 L-carnitine/gamma-butyrobetaine antiporter from Escherichia coli (strain K12) (see 3 papers)
26% identity, 75% coverage: 4:497/658 of query aligns to 7:502/504 of P31553

query
sites
P31553

K

K

S

T

S

G

I

I

N

E

P

P

P

K

V

V

F

F

Y

F

S

P

V

L

F

I

I

V

T

G

L

I

M

L

V

C

M

W

I

L

C

T

A

V

I

R

W

D

P

L

T

D

E

A

A

A

N

N

H

V

F

V

F

I

K

N

S

A

M

V

Q

F

S

S

W

Y

L

V

E

T

A

N

K

V

A

W

G

G

W

W

L

A

Y

F

I

E

L

W

G

Y

V

M

A

V

I

V

F

M

L

L

I

F

F

G

I

W

I

F

F

W

V

L

M

V

V

F

S

G

R

P

F

Y

G

A

D

K

I

K

K

R

L

L

G

G

P

N

D

E

H

P

A

-

V

-

P

P

D

E

Y

F

S

S

Y

T

K

A

S

S

W

W

I

I

A

F

M

M

L

M

F

F

S

A

A

S

G

C

M

T

G

S

I

A

G

A

L

V

M

L

F

F

F

W

G

G

V

S

A

I

E

E

P

I

V

Y

M
x
Y

H

Y

Y

I

L

S

A

T

P

P

-

F

D

G

A

L

T

E

P

P

E

N

S

S

L

T

A

G

A

A

A

K

K

E

E

L

A

G

M

L

K

A

I

Y

T

S

F

L

F

F

H

H

W
|
W

G

G

I

P

H

L

A

P

W

W

A

A

I

T

Y

Y

G

S

V

F

V

L

A

S

L

V

S

A

L

F

A

A

Y

Y

F

F

A

F

Y

F

R

V

H

R

K

K

L

M

P

E

L

V

L

I

-

R

P

P

R

S

S

S

A

T

L

L

Y

V

P

P

L

L

I

V

G

G

E

E

R

K

-

H

I

A

H

K

G

G

P

L

I

F

G

G

H

T

C

I

V

V

D

D

T

N

F

F

A

Y

V

L

L

V

G

A

T

L

M

I

F

F

G

A

V

M

A

G

T

T

S

S

L

L

G

G

F

L

G

A

V

T

L

P

Q

L

V

V

N

T

S

E

G

C

L

M

S

Q

Y

W

L

L

F

F

E

-

Q

G

L

I

P

P

N

H

N

T

T

L

T

Q

V

L

Q

D

V

A

S

I

L

I

I

I

I

T

G

C

I

W

T

I

L

I

L

L

A

N

T

A

L

I

S

C

V

V

F

A

S

C

G

G

L

L

D

Q

K

K

G

G

V

V

K

R

R

I

L

A

S

S

E

D

L

V

N

R

L

S

G

Y

L

L

A

S

L

F

I

L

L

M

L

L

L

G

I

W

V

V

L

F

I

I

L

V

G

S

P

G

T

A

V

S

M

F

L

I

L

M

Q

N

A

Y

F

F

V

T

Q
x
D

N

S

T

V

G

G

S

M

Y

L

L

L

S
x
M

D

Y

I

L

V

P

N
x
R

K

M

T

L

F

F

N

Y

L
x
T

Y

D

A

P

Y

I

Q

A

H

-

K

K

E

G

D

G

W

F

L

P

G

Q

G
|
G

W
|
W

T

T

L

V

L

F

Y

Y

W

W

G

A

W
|
W

I

V

S

I

W
x
Y

S
x
A

P
x
I

F
x
Q

V
x
M

G

S

T

I

F

F

I

L

A

A

R

R

V

I

S

S

R

R

G

G

R

R

T

T

I

V

R

R

E

E

F

L

L

C

V

F

G

G

I

M

L

V

F

L

V

G

P

L

S

T

A

A

L

S

T

T

F

W

L

I

W

L

M

W

T

T

V

V

F

L

G

G

N

S

S

N

A

T

I

L

D

L

A

L

I

I

M

D

N

K

Q

N

G

I

A

I

T

N

Y

I

L

P

S

N

E

L

A

I

V

E

N

Q

T

Y

N

G

V

V

P

A

V

R

A

A

L

I

F

I

V

E

F

T

F

W

E

A

H

A

M

L

P

P

F

L

-

S

-

T

P

A

H

T

L

M

L

W

S

G

G

F

I

F

G

I

I

L

C

C

L

F

V

I

V

A

T

T

F

V

F

T

V

L

T

V

S

N

S

A

D

C

S

S

G

Y

S

T

L

L

V

A

I

M

D

S

N

T

L

C

T

R

S

E

G

V

G

R

D

D

-

G

N

E

A

P

P

P

V

L

W

L

Q

V

R

R

V

I

F

G

W

W

A

S

L

I

L

L

Q

V

G

G

V

I

A

G

S

I

V

V

L

L

L

A

A

L

G

G

L

L

Q

K

A

P

L

I

Q

Q

T

T

A

A

A

I

I

I

A

A

S

G

A

G

M

C

P

P

F

L

L

F

C

F

V

V

M

N

L

I

L

M

M

V

C

T

L

L

G

S

L

F

Y

I

K

K

A

D

L

A

K

K

D

Q

D

N

W

W

Y114 (≠ M113) binding ; mutation to L: Small decrease in transport activity.
W142 (= W140) binding
D288 (≠ Q285) mutation to A: Retains 70% of transport activity. Forms mostly monomers.; mutation to R: Abolishes transport activity. Forms mostly monomers.; mutation to W: Retains 4% of transport activity. Forms mostly monomers.
M295 (≠ S292) mutation to E: Does not affect transport activity. Forms mostly monomers. Can also form small amounts of homodimers and homotrimers.
R299 (≠ N296) mutation to A: Does not affect transport activity. Forms mostly monomers. Can also form small amounts of homodimers and homotrimers. Shows a high tendency to aggregate.
T304 (≠ L301) mutation to A: Does not affect transport activity. Forms mostly monomers. Shows a high tendency to aggregate.
GW 315:316 (= GW 313:314) binding
W316 (= W314) mutation to L: Decrease in transport activity.
W323 (= W321) binding ; mutation to L: Abolishes transport activity.
WW 323:324 (= WW 321:322) binding
W324 (= W322) mutation to L: Abolishes transport activity.
Y327 (≠ W325) mutation to L: Strong decrease in transport activity.
YAIQ 327:330 (≠ WSPF 325:328) binding
Q330 (≠ F328) mutation to L: Decrease in transport activity.
M331 (≠ V329) binding

2wsxA Crystal structure of carnitine transporter from escherichia coli (see paper)
26% identity, 75% coverage: 5:497/658 of query aligns to 1:495/496 of 2wsxA

query
sites
2wsxA

S

T

S

G

I

I

N

E

P

P

P

K

V

V

F

F

Y

F

S

P

V

L

F

I

I

V

T

G

L

I

M

L

V

C

M

W

I

L

C

T

A

V

I

R

W

D

P

L

T

D

E

A

A

A

N

N

H

V

F

V

F

I

K

N

S

A

M

V

Q

F

S

S

W

Y

L

V

E

T

A

N

K

V

A

W

G

G

W

W

L

A

Y

F

I

E

L

W

G

Y

V

M

A

V

I

V

F

M

L

L

I

F

F

G

I

W

I

F

F

W

V

L

M

V

V

F

S

G

R

P

F

Y

G

A

D

K

I

K

K

R

L

L

G

G

P

E

D

E

H

P

A

-

V

-

P

P

D

E

Y

F

S

S

Y

T

K

A

S

S

W

W

I

I

A

F

M

M

L

M

F

F

S

A

A

S

G

C

M

T

G

S

I

A

G

A

L

V

M

L

F

F

F

W

G

G

V

S

A

I

E

E

P

I

V

Y

M

Y

H

Y

Y

I

L

S

A

T

P

P

-

F

D

G

A

L

T

E

P

P

E

N

S

S

L

T

A

G

A

A

A

K

K

E

E

L

A

G

M

L

K

A

I

Y

T

S

F

L

F

F

H

H

W
|
W

G

G

I

P

H

L

A

P

W

W

A

A

I

T

Y

Y

G

S

V

F

V

L

A

S

L

V

S

A

L

F

A

A

Y

Y

F

F

A

F

Y

F

R

V

H

R

K

K

L

M

P

E

L

V

L

I

-

R

P

P

R

S

S

S

A

T

L

L

Y

V

P

P

L

L

I

V

G

G

E

E

R

K

-

H

I

A

H

K

G

G

P

L

I

F

G

G

H

T

C

I

V

V

D

D

T

N

F

F

A

Y

V

L

L

V

G

A

T

L

M

I

F

F

G

A

V

M

A

G

T

T

S

S

L

L

G

G

F

L

G

A

V

T

L

P

Q

L

V

V

N

T

S

E

G

C

L

M

S

Q

Y

W

L

L

F

F

E

-

Q

G

L

I

P

P

N

H

N

T

T

L

T

Q

V

L

Q

D

V

A

S

I

L

I

I

I

I

T

G

C

I

W

T

I

L

I

L

L

A

N

T

A

L

I

S

C

V

V

F

A

S

C

G

G

L

L

D

Q

K

K

G

A

V

V

K

R

R

I

L

A

S

S

E

D

L

V

N

R

L

S

G

Y

L

L

A

S

L

F

I

L

L

M

L

L

L

G

I

W

V

V

L

F

I

I

L

V

G

S

P

G

T

A

V

S

M

F

L

I

L

M

Q

N

A

Y

F

F

V

T

Q

D

N

S

T

V

G

G

S

M

Y

L

L

L

S

M

D

Y

I

L

V

P

N

R

K

M

T

L

F

F

N

Y

L

T

Y

D

A

P

Y

I

Q

A

H

-

K

K

E

G

D
x
G

W

F

L

P

G

Q

G
|
G

W

W

T

T

L

V

L

F

Y

Y

W

W

G

A

W
|
W

W

W

I

V

S

I

W
x
Y

S

A

P

I

F

Q

V
x
M

G

S

T

I

F

F

I

L

A

A

R

R

V

I

S

S

R

R

G

G

R

R

T

T

I

V

R

R

E

E

F

L

L

C

V

F

G

G

I

M

L

V

F

L

V

G

P

L

S

T

A

A

L

S

T

T

F

W

L

I

W

L

M

W

T

T

V

V

F

L

G

G

N

S

S

N

A

T

I

L

D

L

A

L

I

I

M

D

N

K

Q

N

G

I

A

L

T

N

Y

I

L

P

S

N

E

L

A

I

V

E

N

Q

T

Y

N

G

V

V

P

A

V

R

A

A

L

I

F

I

V

E

F

T

F

W

E

A

H

A

M

L

P

P

F

L

-

S

-

T

P

A

H

T

L

M

L

W

S

G

G

F

I

F

G

I

I

L

C

C

L

F

V

I

V

A

T

T

F

V

F

T

V

L

T

V

S

N

S

A

D

C

S

S

G

Y

S

T

L

L

V

A

I

M

D

S

N

T

L

C

T

R

S

E

G

V

G

R

D

D

-

G

N

E

N

D

A

P

P

P

V

L

W

L

Q

V

R

R

V

I

F

G

W

W

A

S

L

I

L

L

Q

V

G

G

V

I

A

G

S

I

V

V

L

L

L

A

A

L

G

G

L

L

Q

K

A

P

L

I

Q
|
Q

T

T

A

A

A

I

I

I

A

A

S

G

A

G

M

C

P

P

F

L

L

F

C

F

V

V

M

N

L

I

L

M

M

V

C

T

L

L

G

S

L

F

Y

I

K

K

A

D

L

A

K

K

D

Q

D

N

W

W

binding 3-carboxy-n,n,n-trimethylpropan-1-aminium: W135 (= W140), G304 (≠ D309), G308 (= G313), W316 (= W321), Y320 (≠ W325), M324 (≠ V329), Q466 (= Q468)

3hfxA Crystal structure of carnitine transporter (see paper)
27% identity, 73% coverage: 15:497/658 of query aligns to 2:491/493 of 3hfxA

query
sites
3hfxA

S

K

V

V

F

F

I

P

T

P

L

L

M

I

V

I

M

V

I

G

C

I

A

L

I

C

W

W

P

L

T

T

-

V

-

R

-

D

-

L

-

D

E

A

A

A

N

N

H

V

F

V

F

I

K

N

S

A

M

V

Q

F

S

S

W

Y

L

V

E

T

A

N

K

V

A

W

G

G

W

W

L

A

Y

F

I

E

L

W

G

Y

V

M

A

V

I

V

F

M

L

L

I

F

F

G

I

W

I

F

F

W

V

L

M

V

V

F

S

G

R

P

F

Y

G

A

D

K

I

K

K

R

L

L

G

G

P

N

D

E

H

P

A

-

V

-

P

P

D

E

Y

F

S

S

Y

T

K

A

S

S

W

W

I

I

A

F

M

M

L

M

F

F

S

A

A

S

G

C

M

T

G

S

I

A

G

A

L

V

M

L

F

F

F

W

G

G

V

S

A

I

E

E

P

I

V

Y

M
x
Y

H

Y

Y

I

L

S

A

T

P

P

-

F

D

G

A

L

T

E

P

P

E

N

S

S

L

T

A

G

A

A

A

K

K

E

E

L

A

G

M

L

K

A

I

Y

T

S

F

L

F

F

H

H

W
|
W

G

G

I

P

H

L

A

P

W

W

A

A

I

T

Y

Y

G

S

V

F

V

L

A

S

L

V

S

A

L

F

A

A

Y

Y

F

F

A

F

Y

F

R

V

H

R

K

K

L

M

P

E

L

V

L

I

-

R

P

P

R

S

S

S

A

T

L

L

Y

V

P

P

L

L

I

V

G

G

E

E

R

K

-

H

I

A

H

K

G

G

P

L

I

F

G

G

H

T

C

I

V

V

D

D

T

N

F

F

A

Y

V

L

L

V

G

A

T

L

M

I

F

F

G

A

V

M

A

G

T

T

S

S

L

L

G

G

F

L

G

A

V

T

L

P

Q

L

V

V

N

T

S

E

G

C

L

M

S

Q

Y

W

L

L

F

F

E

-

Q

G

L

I

P

P

N

H

N

T

T

L

T

Q

V

L

Q

D

V

A

S

I

L

I

I

I

I

T

G

C

I

W

T

I

L

I

L

L

A

N

T

A

L

I

S

C

V

V

F

A

S

C

G

G

L

L

D

Q

K

K

G

G

V

V

K

R

R

I

L

A

S

S

E

D

L

V

N

R

L

S

G

Y

L

L

A

S

L

F

I

L

L

M

L

L

L

G

I

W

V

V

L

F

I

I

L

V

G

S

P

G

T

A

V

S

M

F

L

I

L

M

Q

N

A

Y

F

F

V

T

Q

D

N

S

T

V

G

G

S

M

Y

L

L

L

S

M

D

Y

I

L

V

P

N

R

K

M

T

L

F

F

N

Y

L

T

Y

D

A

P

Y

I

Q

A

H

-

K

K

E

G

D

G

W

F

L

P

G

Q

G

G

W
|
W

T

T

L

V

L

F

Y

Y

W

W

G

A

W
|
W

I

V

S

I

W
x
Y

S

A

P

I

F
x
Q

V

M

G

S

T

I

F
|
F

I

L

A

A

R

R

V

I

S

S

R

R

G

G

R

R

T

T

I

V

R

R

E

E

F

L

L

C

V

F

G

G

I

M

L

V

F

L

V

G

P

L

S

T

A

A

L

S

T

T

F

W

L

I

W

L

M

W

T

T

V

V

F

L

G

G

N

S

S

N

A

T

I

L

D

L

A

L

I

I

M

D

N

K

Q

N

G

I

A

I

T

N

Y

I

L

P

S

N

E

L

A

I

V

E

N

Q

T

Y

N

G

V

V

P

A

V

R

A

A

L

I

F

I

V

E

F

T

F

W

E

A

H

A

M

L

P

P

F

L

-

S

-

T

P

A

H

T

L

M

L

W

S

G

G

F

I

F

G

I

I

L

C

C

L

F

V

I

V

A

T

T

F

V

F

T

V

L

T

V

S

N

S

A

D

C

S

S

G

Y

S

T

L

L

V

A

I

M

D

S

N

T

L

C

T

R

S

E

G

V

G

R

D

D

-

G

N

E

A

P

P

P

V

L

W

L

Q

V

R

R

V

I

F

G

W

W

A

S

L

I

L

L

Q

V

G

G

V

I

A

G

S

I

V

V

L

L

L

A

A

L

G

G

L

L

Q

K

A

P

L

I

Q

Q

T

T

A

A

A

I

I

I

A

A

S

G

A

G

M

C

P

P

F

L

L

F

C

F

V

V

M

N

L

I

L

M

M

V

C

T

L

L

G

S

L

F

Y

I

K

K

A

D

L

A

K

K

D

Q

D

N

W

W

binding carnitine: Y103 (≠ M113), W131 (= W140), W305 (= W314), W312 (= W321), W313 (= W322), Y316 (≠ W325), Y316 (≠ W325), Q319 (≠ F328), F323 (= F332)

Query Sequence

>7025568 FitnessBrowser__ANA3:7025568
MSIKSSINPPVFYSSVFFITLMVMICAIWPTEANHFFKSMQSWLEAKAGWLYILGVAIFL
IFIIFVMVSRFGDIKLGPDHAVPDYSYKSWIAMLFSAGMGIGLMFFGVAEPVMHYLAPPD
ATPESLAAAKEAMKITFFHWGIHAWAIYGVVALSLAYFAYRHKLPLLPRSALYPLIGERI
HGPIGHCVDTFAVLGTMFGVATSLGFGVLQVNSGLSYLFEQLPNNTTVQVSLIIGITLLA
TLSVFSGLDKGVKRLSELNLGLALILLLIVLILGPTVMLLQAFVQNTGSYLSDIVNKTFN
LYAYQHKEDWLGGWTLLYWGWWISWSPFVGTFIARVSRGRTIREFLVGILFVPSALTFLW
MTVFGNSAIDAIMNQGATYLSEAVNTNVPVALFVFFEHMPFPHLLSGIGICLVVTFFVTS
SDSGSLVIDNLTSGGDNNAPVWQRVFWALLQGVVASVLLLAGGLQALQTAAIASAMPFLC
VMLLMCLGLYKALKDDWLKINSVQLHTTSVQYAKTNISWEERIEVLVSHPTEDEARIFLN
NVATPALSKVCQQFMSKGLTADLEYLDGKVRLVISNEAYQPFVYGVRMRCFEIVNPVGEE
LEQGNNWYYRAEVYLEQGGQHYDVMGYTEEQLLADVVTQYEKYLHYLHLSNADQGHVA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory