SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 7025757 FitnessBrowser__ANA3:7025757 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

8ahxC Cryo-em structure of the nitrogen-fixation associated nadh:ferredoxin oxidoreductase rnf from azotobacter vinelandii
35% identity, 14% coverage: 313:376/464 of query aligns to 368:423/476 of 8ahxC

query
sites
8ahxC

C
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C

I
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I

R
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R

C
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C

G
x
A

G
x
S

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C

L

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N

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C

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L

binding flavin mononucleotide: C410 (= C363)
binding iron/sulfur cluster: C368 (= C313), I369 (= I314), R370 (= R315), C371 (= C316), A372 (≠ G317), S373 (≠ G318), C374 (= C319), C378 (= C323), P379 (= P324), C407 (= C360), I408 (≠ T361), L409 (= L362), C410 (= C363), G411 (= G364), C413 (= C366), C417 (= C370), P418 (= P371), S419 (≠ T372)

Sites not aligning to the query:

binding flavin mononucleotide: 137, 139, 163, 167, 238, 239, 266, 267, 334

5odcC Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus at 2.3 a resolution (see paper)
29% identity, 22% coverage: 293:393/464 of query aligns to 15:110/184 of 5odcC

query
sites
5odcC

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C

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C

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G
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binding iron/sulfur cluster: C34 (= C313), Y35 (≠ I314), Q36 (≠ R315), C37 (= C316), G38 (= G317), C40 (= C319), C44 (= C323), P45 (= P324), Y52 (≠ G331), T54 (≠ S333), C77 (= C360), T78 (= T361), T79 (≠ L362), C80 (= C363), Y81 (≠ G364), C83 (= C366), C87 (= C370), P88 (= P371), V91 (= V374)

5odcI Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus at 2.3 a resolution (see paper)
29% identity, 22% coverage: 293:393/464 of query aligns to 15:110/173 of 5odcI

query
sites
5odcI

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binding magnesium ion: R53 (≠ Y332), D73 (= D352)
binding iron/sulfur cluster: C34 (= C313), Y35 (≠ I314), Q36 (≠ R315), C37 (= C316), G38 (= G317), C40 (= C319), C44 (= C323), P45 (= P324), Y52 (≠ G331), T54 (≠ S333), C77 (= C360), T78 (= T361), T79 (≠ L362), C80 (= C363), Y81 (≠ G364), C83 (= C366), C87 (= C370), V91 (= V374)

Query Sequence

>7025757 FitnessBrowser__ANA3:7025757
MAYQHNHEATGSQVHAHKADIFCRDETRVDWHSKALWVLREKRDRAAGSLPEWEQLRQLG
SEIKLHTLTNLAQYLETFEQNCLANGIKVHWAKDGAEHNRIVHDILASHKVKKLVKSKSM
LTEECHLNPYLEQRGIEVIDTDLGERIIQLAKMPPSHIVVPAIHMKKEEVGDLFHDKLGT
KAGESDPLYLTRAARAHLREQFLSADAAMTGVNMAIADKGAVVVCTNEGNADMGANLPKL
QLHSMGIDKIVPDIDSAAVLLRTLARNATGQPVTTYSAFYRGPQVDGEMHVIIVDNGRTE
MLKDKILAESLKCIRCGGCLNTCPVYRRSGGYSYNYTIPGPIGIAVGAKHDDTNSIAWAC
TLCGSCTYVCPTKVPLDKIIFHHRRLKAEAGQLPYGKNGYMPLVGKFMASTTLLNCSMGA
ARTALRILPGSLLKPFSGAWGKYRELPVAPNSSFEAWFKKHRSL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory