SitesBLAST
Comparing 7025945 FitnessBrowser__ANA3:7025945 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 9 hits to proteins with known functional sites (download)
7cyxA Crystal strcuture of glycine oxidase from bacillus cereus atcc 14579 (see paper)
22% identity, 83% coverage: 31:387/428 of query aligns to 3:347/363 of 7cyxA
- binding flavin-adenine dinucleotide: I7 (= I35), G8 (= G36), G10 (= G38), V11 (≠ Y39), I12 (≠ T40), V30 (≠ L58), E31 (= E59), K32 (≠ A60), E38 (≠ G66), A39 (= A67), S40 (= S68), A43 (≠ N71), G45 (= G73), L46 (≠ Q74), V171 (= V210), G200 (= G237), G201 (≠ N238), W203 (≠ Y240), G298 (= G340), R300 (≠ F342), P301 (≠ L343), Y326 (vs. gap), R327 (vs. gap), N328 (vs. gap), G329 (= G369), I330 (≠ V370)
5i39A High resolution structure of l-amino acid deaminase from proteus vulgaris with the deletion of the specific insertion sequence (see paper)
23% identity, 85% coverage: 29:392/428 of query aligns to 24:374/383 of 5i39A
- active site: F66 (≠ N71), Q69 (= Q74), A70 (≠ I75), Q248 (vs. gap), P267 (≠ D288)
- binding flavin-adenine dinucleotide: V30 (≠ I35), G31 (= G36), G33 (= G38), I34 (≠ Y39), L35 (≠ T40), V53 (≠ L58), E54 (= E59), K55 (≠ A60), Q62 (≠ A67), S63 (= S68), F66 (≠ N71), Y67 (≠ G72), Q69 (= Q74), A196 (= A209), A197 (≠ V210), G226 (= G237), G227 (≠ N238), W229 (≠ Y240), Q248 (vs. gap), Q250 (≠ T257), G321 (= G340), M323 (≠ F342), T348 (≠ S366), G349 (= G367), W350 (≠ H368), G351 (= G369), M352 (≠ V370), T353 (= T371)
1ng3A Complex of thio (glycine oxidase) with acetyl-glycine (see paper)
24% identity, 71% coverage: 93:397/428 of query aligns to 57:359/364 of 1ng3A
- binding acetylamino-acetic acid: Y246 (= Y285), R302 (≠ F342), R329 (≠ G367)
- binding flavin-adenine dinucleotide: V174 (= V210), S202 (≠ G237), G203 (≠ N238), W205 (≠ Y240), F209 (≠ L244), G300 (= G340), R302 (≠ F342), H327 (≠ C365), R329 (≠ G367), N330 (≠ H368), G331 (= G369), I332 (≠ V370)
- binding phosphate ion: R89 (≠ D121), R254 (= R297)
Sites not aligning to the query:
- active site: 47, 48, 49
- binding flavin-adenine dinucleotide: 11, 13, 15, 33, 34, 35, 41, 42, 43, 46, 47, 48, 49
O31616 Glycine oxidase; GO; EC 1.4.3.19 from Bacillus subtilis (strain 168) (see 3 papers)
24% identity, 71% coverage: 93:397/428 of query aligns to 57:359/369 of O31616
- V174 (= V210) binding
- H244 (≠ Y278) mutation to A: 2-fold decrease in catalytic efficiency on glycine and similar catalytic efficiency on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and R-54.
- R302 (≠ F342) binding
- 327:333 (vs. 365:371, 14% identical) binding
- R329 (≠ G367) binding
Sites not aligning to the query:
- 14:15 binding
- 34:35 binding
- 42:43 binding
- 47:49 binding
- 51 G→R: 130-fold decrease in catalytic efficiency on glycine and 28-fold increase in that on glyphosate.; G→S: 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with R-54 and A-244.
- 54 A→R: 20-fold decrease in catalytic efficiency on glycine and 34-fold increase in that on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and A-244.
S5FMM4 Glycine oxidase; GO; BliGO; EC 1.4.3.19 from Bacillus licheniformis (see paper)
24% identity, 83% coverage: 43:397/428 of query aligns to 18:359/369 of S5FMM4
- G51 (≠ V76) mutation to S: Shows 4.3- and 107-fold increase of affinity to glyphosate and glycine, respectively. Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with R-54, R-81, C-202, V-332 and V-342.
- A54 (≠ Y79) mutation to R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-81, C-202, V-332 and V-342.
- K81 (≠ G106) mutation to R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, C-202, V-332 and V-342.
- S202 (≠ G237) mutation to C: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, V-332 and V-342.
- I332 (≠ V370) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-342.
- M342 (≠ I380) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-332.
3if9A Crystal structure of glycine oxidase g51s/a54r/h244a mutant in complex with inhibitor glycolate (see paper)
24% identity, 71% coverage: 93:397/428 of query aligns to 57:359/364 of 3if9A
- binding flavin-adenine dinucleotide: P173 (≠ A209), V174 (= V210), S202 (≠ G237), G203 (≠ N238), W205 (≠ Y240), F209 (≠ L244), G300 (= G340), R302 (≠ F342), H327 (≠ C365), F328 (≠ S366), R329 (≠ G367), N330 (≠ H368), G331 (= G369), I332 (≠ V370)
- binding glycolic acid: Y246 (= Y285), R302 (≠ F342), R329 (≠ G367)
Sites not aligning to the query:
- active site: 47, 48, 49
- binding flavin-adenine dinucleotide: 11, 13, 15, 34, 35, 42, 43, 46, 47, 48, 49
5hxwA L-amino acid deaminase from proteus vulgaris (see paper)
26% identity, 55% coverage: 29:264/428 of query aligns to 16:249/433 of 5hxwA
- active site: F58 (≠ N71), Q61 (= Q74), A62 (≠ I75), Q240 (vs. gap)
- binding flavin-adenine dinucleotide: V22 (≠ I35), G23 (= G36), G25 (= G38), I26 (≠ Y39), L27 (≠ T40), E46 (= E59), K47 (≠ A60), E53 (≠ G66), Q54 (≠ A67), S55 (= S68), R57 (= R70), F58 (≠ N71), Y59 (≠ G72), G60 (= G73), Q61 (= Q74), A188 (= A209), A189 (≠ V210), G218 (= G237), G219 (≠ N238), W221 (≠ Y240), Q240 (vs. gap), Q242 (≠ T257)
Sites not aligning to the query:
- active site: 284, 288
- binding cetyl-trimethyl-ammonium: 291, 294, 310, 311, 317, 318, 320, 373, 379, 399, 400
- binding flavin-adenine dinucleotide: 331, 371, 373, 398, 399, 400, 401, 402, 403
1y56B Crystal structure of l-proline dehydrogenase from p.Horikoshii (see paper)
22% identity, 81% coverage: 45:391/428 of query aligns to 20:358/374 of 1y56B
- active site: F44 (≠ G69), G47 (= G72), T48 (≠ G73), H224 (≠ T257), P239 (= P275), G305 (= G340), M338 (≠ T371)
- binding flavin-adenine dinucleotide: I33 (≠ L58), E34 (= E59), K35 (≠ A60), S42 (≠ A67), T43 (≠ S68), R45 (= R70), C46 (≠ N71), G47 (= G72), G49 (≠ Q74), E170 (≠ A209), V171 (= V210), T200 (≠ G237), N201 (= N238), W203 (≠ Y240), G305 (= G340), Y306 (≠ N341), Y307 (≠ F342), G334 (= G367), H335 (= H368), G336 (= G369), F337 (≠ V370), M338 (≠ T371)
- binding flavin mononucleotide: F44 (≠ G69), R45 (= R70), I260 (≠ D295), R301 (≠ Y336), W303 (= W338)
Sites not aligning to the query:
Q9UI17 Dimethylglycine dehydrogenase, mitochondrial; ME2GLYDH; EC 1.5.8.4 from Homo sapiens (Human) (see 4 papers)
22% identity, 57% coverage: 22:267/428 of query aligns to 42:280/866 of Q9UI17
- CV 59:60 (≠ YT 39:40) binding
- EK 80:81 (≠ EA 59:60) binding
- 87:95 (vs. 66:75, 20% identical) binding
- H91 (≠ R70) modified: Tele-8alpha-FAD histidine
- H109 (≠ G91) to R: in DMGDHD; shows 10 fold lower catalytic efficiency due to lower cofactor saturation and reduced thermal stability; dbSNP:rs121908331
- V219 (= V210) binding
- S279 (≠ D266) to P: in dbSNP:rs532964
Sites not aligning to the query:
- 397:402 binding
- 530 A → G: in dbSNP:rs1805073
- 646 S → P: in dbSNP:rs1805074
Query Sequence
>7025945 FitnessBrowser__ANA3:7025945
MSAIPHTGSYYAASANDKVERARLQESIEADVCVIGAGYTGLSAALHLLESGFSVVVLEA
ARIGWGASGRNGGQIVNSYSRDIDTIEKTVGKEQAKLFGQMAFEGGRIIRERIAKYNIDC
DLKDGGVFAAMNEKQMGHLRHQKQLWESHGHVGKLELLDANSIRSVVNTERYVGGMLDKS
GGHIHPLNLALGEARAVESLGGKIFEDSAVLRVDEGDSPVVHTAKGSVKAKFVVVAGNAY
LGKLMPELQAKSMPCGTQVITTEPLDEELAASLLPQDYCVEDCNYLLDYFRLSGDKRLIY
GGGVVYGARDPADIESLIIPNMLKTFPQLKGVKVDYAWTGNFLLTLSRLPQVGRIGKNIY
YSQGCSGHGVTYTHLAGKLIAEMLNGQATRFDAFAALPHYPFPGGHALRVPFSALGAWYY
SLRDKLGI
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory