SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 7026015 FitnessBrowser__ANA3:7026015 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
40% identity, 94% coverage: 10:400/417 of query aligns to 11:420/425 of O59010

query
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O59010

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S65 (≠ A58) mutation to V: Strongly decreased chloride conductance.
R276 (≠ S261) mutation to S: Increased rate of aspartate transport; when associated with R-395.
RSS 276:278 (≠ SSS 261:263) binding
M311 (= M296) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
T314 (≠ C299) binding
V355 (= V341) binding
D394 (= D374) binding
M395 (= M375) mutation to R: Increased rate of aspartate transport; when associated with S-276.
R397 (= R377) mutation to A: Strongly decreased affinity for aspartate.
N401 (= N381) binding
D405 (= D385) mutation to N: Strongly decreased affinity for aspartate.

5e9sA Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
39% identity, 97% coverage: 2:407/417 of query aligns to 1:427/427 of 5e9sA

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binding aspartic acid: R274 (≠ S261), S275 (= S262), S276 (= S263), T313 (≠ C299), G353 (= G340), V354 (= V341), A357 (≠ S344), G358 (≠ A345), D394 (= D374), R397 (= R377), T398 (= T378)
binding decyl-beta-d-maltopyranoside: L194 (≠ Q180), G198 (≠ M184), Y202 (≠ L188)
binding sodium ion: Y87 (≠ F83), T90 (= T86), S91 (≠ G87), S276 (= S263), G305 (= G291), A306 (= A292), T307 (= T293), N309 (= N295), N309 (= N295), M310 (= M296), D311 (= D297), S348 (= S335), I349 (≠ V336), G350 (= G337), T351 (= T338), N401 (= N381), V402 (= V382), D405 (= D385)

6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
39% identity, 95% coverage: 10:407/417 of query aligns to 6:424/424 of 6zl4A

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A

I

Y

A

A

F

M

I

I

A

L

A

G

I

I

D

D

R

A

I

I

I

L

D

D

M

M

I

G

R
|
R

T
|
T

A

M

T

V

N

N

V

V

T

T

G

G

D

D

M

L

M

A

T

G

A

T

V

V

V

I

I

V

G

A

K

K

S

T

E

E

N

K

E

E

L

L

D

D

V

E

E

S

Q

K

F

W

Y

I

S

S

N

H

binding decyl-beta-d-maltopyranoside: L191 (≠ Q180), G195 (≠ M184), R282 (= R272)
binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (≠ S261), S272 (= S262), S273 (= S263), M307 (= M296), T310 (≠ C299), G353 (= G343), A354 (≠ S344), R394 (= R377), T395 (= T378)

Sites not aligning to the query:

binding decyl-beta-d-maltopyranoside: 3

6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
39% identity, 95% coverage: 10:407/417 of query aligns to 7:425/425 of 6zgbA

query
sites
6zgbA

P

P

F

V

W

L

Q

W

K

K

V

I

L

L

A

W

G

G

F

L

I

V

L

L

G

G

A

A

L

V

V

F

G

G

V

L

L

I

L

A

G

G

E

H

-

F

-

G

-

Y

-

A

-

G

-

A

T

V

A

K

T

T

V

Y

L

I

K

K

P

P

L

F

G

G

D

D

L

L

F

F

I

V

S

R

A

L

I

L

K

K

M

M

L

L

V

V

A

M

P

P

L

I

V

V

F

L

C

A

A

S

I

L

V

V

S

G

I

A

T

A

S

S

L

I

G

-

S

S

Q

P

T

A

N

R

L

L

K

G

R

R

L

V

S

G

L

V

K

K

T

I

L

V

G

V

M

Y

F

Y

M

L

L

A

T

T

G

S

T

A

V

M

A

A

S

V

L

F

I

F

G

G

L

L

A

I

V

V

G

G

S

R

L

L

I

F

D

N

M

V

G

G

G

A

T

N

M

V

Q

N

L

L

A

G

-

S

-

G

T

T

T

G

E

K

V

A

R

I

E

E

R

A

N

Q

I

P

P

P

G

S

F

L

A

V

Q

Q

V

T

L

L

D

N

M

I

I

V

P

P

V

T

N

N

P

P

F

F

A

A

S

S

L

L

A

A

D

K

G

G

K

E

V

V

L

L

Q

P

I

V

I

I

V

F

F

F

A

A

A

I

L

I

V

L

G

G

I

I

A

A

I

I

-

T

-

Y

-

L

-

M

-

N

-

R

-

N

-

E

N

E

K

R

V

V

G

R

E

K

K

S

A

A

E

E

P

T

L

L

K

L

R

R

T

V

I

F

E

D

A

G

G

L

A

A

E

E

V

A

M

M

F

Y

Q

L

L

I

T

V

R

G

M

G

V

V

L

M

K

Q

L

Y

T

A

P

P

I

I

G

G

V

V

F

F

G

A

L

L

M

I

A

A

W

Y

V

V

V

M

G

A

E

E

Y

Q

G

G

L

V

S

R

T

V

L

V

L

G

P

P

L

L

G

A

K

K

F

V

I

V

A

G

A

A

I

V

Y

Y

I

T

A

G

A

L

L

F

I

L

H

Q

M

I

I

V

F

I

V

T

Y

Y

G

F

G

I

L

L

V

L

K

K

F

-

A

V

A

F

G

G

L

I

S

D

P

P

V

I

Q

K

F

F

F

I

R

R

K

K

A

A

M

K

P

D

A

A

Q

M

L

I

V

T

A

A

F

F

S

V

T

T

S
x
R

S
|
S

F

S

G

G

T

T

L

L

P

P

A

V

S

T

T

M

R

R

-

V

A

A

V

E

E

E

T

E

M

M

G

G

V

V

S

D

K

K

R

G

Y

I

S

F

A

S

F

F

V

T

M

L

P

P

L

L

G

G

A

A

T

T

M

I

N

N

M
|
M

D

D

G

G

C

T

G

-

G

A

I

L

Y

Y

P

Q

A

G

I

V

A

T

A

V

I

L

F

F

I

V

A

A

Q

N

I

A

Y

I

G

G

I

H

P

P

L

L

D

T

T

L

L

G

D

Q

Y

Q

V

L

M

V

I

V

A

V

V

L

T

T

A

A

T

V

V

L

A

A

S

S

V

I

G

G

T

T

A

A

G

G

V

V

P

P

G

G

S

A

A

G

M

A

V

I

M

M

L

L

T

A

V

M

T

V

L

L

G

Q

V

S

I

V

G

G

L

L

P

D

L

L

E

T

-

P

-

G

-

S

-

P

-

V

-

A

-

L

G

A

I

Y

A

A

F

M

I

I

A

L

A

G

I

I

D

D

R

A

I

I

I

L

D
|
D

M

M

I

G

R
|
R

T
|
T

A

M

T

V

N

N

V

V

T

T

G

G

D

D

M

L

M

A

T

G

A

T

V

V

V

I

I

V

G

A

K

K

S

T

E

E

N

K

E

E

L

L

D

D

V

E

E

S

Q

K

F

W

Y

I

S

S

N

H

binding (2~{S},3~{S})-2-azanyl-3-[(2-nitrophenyl)methoxy]butanedioic acid: R272 (≠ S261), S273 (= S262), S274 (= S263), M308 (= M296), D392 (= D374), R395 (= R377), T396 (= T378)

6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor (see paper)
39% identity, 97% coverage: 2:406/417 of query aligns to 1:426/426 of 6xwnB

query
sites
6xwnB

I

L

L

L

Q

R

T

R

I

Y

S

L

R

D

I

Y

P

P

F

V

W

L

Q

W

K

K

V

I

L

L

A

W

G

G

F

L

I

V

L

L

G

G

A

A

L

V

V

F

G

G

V

L

L

I

L

A

G

G

E

H

-

F

-

G

-

Y

-

A

-

G

-

A

T

V

A

K

T

T

V

Y

L

I

K

K

P

P

L

F

G

G

D

D

L

L

F

F

I

V

S

R

A

L

I

L

K

K

M

M

L

L

V

V

A

M

P

P

L

I

V

V

F

L

C

A

A

S

I

L

V

V

S

G

I

A

T

A

S

S

L

I

G

-

S

S

Q

P

T

A

N

R

L

L

K

G

R

R

L

V

S

G

L

V

K

K

T

I

L

V

G

V

M

Y

F

Y

M

L

L

A

T

T

G

S

T

A

V

M

A

A

S

V

L

F

I

F

G

G

L

L

A

I

V

V

G

G

S

R

L

L

I

F

D

N

M

V

G

G

G

A

T

N

M

V

Q

N

L

L

A

G

-

S

-

G

T

T

T

G

E

K

V

A

R

I

E

E

R

A

N

Q

I

P

P

P

G

S

F

L

A

V

Q

Q

V

T

L

L

D

N

M

I

I

V

P

P

V

T

N

N

P

P

F

F

A

A

S

S

L

L

A

A

D

K

G

G

K

E

V

V

L

L

Q

P

I

V

I

I

V

F

F

F

A

A

A

I

L

I

V

L

G

G

I

I

A

A

I

I

-

T

-

Y

-

L

-

M

-

N

-

R

-

N

-

E

N

E

K

R

V

V

G

R

E

K

K

S

A

A

E

E

P

T

L

L

K

L

R

R

T

V

I

F

E

D

A

G

G

L

A

A

E

E

V

A

M

M

F

Y

Q

L

L

I

T

V

R

G

M

G

V

V

L

M

K

Q

L

Y

T

A

P

P

I

I

G

G

V

V

F

F

G

A

L

L

M

I

A

A

W

Y

V

V

V

M

G

A

E

E

Y

Q

G

G

L

V

S

R

T

V

L

V

L

G

P

P

L

L

G

A

K

K

F

V

I

V

A

G

A

A

I

V

Y

Y

I

T

A

G

A

L

L

F

I

L

H

Q

M

I

I

V

F

I

V

T

Y

Y

G

F

G

I

L

L

V

L

K

K

F

-

A

V

A

F

G

G

L

I

S

D

P

P

V

I

Q

K

F

F

F

I

R

R

K

K

A

A

M

K

P

D

A

A

Q

M

L

I

V

T

A

A

F

F

S

V

T

T

S

R

S
|
S

F

S

G

G

T

T

L

L

P

P

A

V

S

T

T

M

R

R

-

V

A

A

V

E

E

E

T

E

M

M

G

G

V

V

S

D

K

K

R

G

Y

I

S

F

A

S

F

F

V

T

M

L

P

P

L

L

G

G

A

A

T

T

M

I

N

N

M
|
M

D

D

G

G

C
x
T

G

-

G

A

I

L

Y

Y

P

Q

A

G

I

V

A

T

A

V

I

L

F

F

I

V

A

A

Q

N

I

A

Y

I

G

G

I

H

P

P

L

L

D

T

T

L

L

G

D

Q

Y

Q

V

L

M

V

I

V

A

V

V

L

T

T

A

A

T

V

V

L

A

A

S

S

V

I

G

G

T

T

A

A

G

G

V

V

P

P

G

G

S

A

A

G

M

A

V

I

M

M

L

L

T

A

V

M

T

V

L

L

G

Q

V

S

I

V

G

G

L

L

P

D

L

L

E

T

-

P

-

G

-

S

-

P

-

V

-

A

-

L

G

A

I

Y

A

A

F

M

I

I

A

L

A

G

I

I

D

D

R

A

I

I

I

L

D
|
D

M

M

I

G

R
|
R

T

T

A

M

T

V

N

N

V

V

T

T

G

G

D

D

M

L

M

A

T

G

A

T

V

V

V

I

I

V

G

A

K

K

S

T

E

E

N

K

E

E

L

L

D

D

V

E

E

S

Q

K

F

W

Y

I

S

S

binding (3s)-3-(benzyloxy)-l-aspartic acid: S275 (= S262), S276 (= S263), M310 (= M296), T313 (≠ C299), D394 (= D374), R397 (= R377)

6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
39% identity, 95% coverage: 10:407/417 of query aligns to 2:416/416 of 6r7rA

query
sites
6r7rA

P

P

F

V

W

L

Q

W

K

K

V

I

L

L

A

W

G

G

F

L

I

V

L

L

G

G

A

A

L

V

V

F

G

G

V

L

L

I

L

A

G

G

E
x
H

-

F

-

G

-

Y

-

A

-

G

-

A

T

V

A

K

T

T

V

Y

L

I

K

K

P

P

L

F

G

G

D

D

L

L

F

F

I

V

S

R

A

L

I

L

K

K

M

M

L

L

V

V

A

M

P

P

L

I

V

V

F

L

C

A

A

S

I

L

V

V

S

G

I

A

T

A

S

S

L

I

G

-

S

S

Q

P

T

A

N

R

L

L

K

G

R

R

L

V

S

G

L

V

K

K

T

I

L

V

G

V

M

Y

F

Y

M

L

L

A

T

T

G

S

T

A

V

M

A

A

S

V

L

F

I

F

G

G

L

L

A

I

V

V

G

G

S

R

L

L

I

F

D

N

M

V

G

G

G

A

T

N

M

V

Q

N

L

L

A

G

T

S

T

G

E

T

V

G

R

A

E

Q

R

P

N

-

I

-

P

P

G

S

F

L

A

V

Q

Q

V

T

L

L

D

N

M

I

I

V

P

P

V

T

N

N

P

P

F

F

A

A

S

S

L

L

A

A

D

K

G

G

K

E

V

V

L

L

Q

P

I

V

I

I

V

F

F

F

A

A

A

I

L

I

V

L

G

G

I

I

A

A

I

I

-

T

-

Y

-

L

-

M

-

N

-

R

-

N

-

E

N

E

K

R

V

V

G

R

E

K

K

S

A

A

E

E

P

T

L

L

K

L

R

R

T

V

I

F

E

D

A

G

G

L

A

A

E

E

V

A

M

M

F

Y

Q

L

L

I

T

V

R

G

M

G

V

V

L

M

K

Q

L

Y

T

A

P

P

I

I

G

G

V

V

F

F

G

A

L

L

M

I

A

A

W

Y

V

V

V

M

G

A

E

E

Y

Q

G

G

L

V

S

R

T

V

L
x
V

L

G

P

P

L

L

G
x
A

K

K

F

V

I

V

A

G

A

A

I

V

Y

Y

I

T

A

G

A

L

L

F

I

L

H

Q

M

I

I

V

F

I

V

T

Y

Y

G

F

G

I

L

L

V

L

K

K

F

-

A

V

A

F

G

G

L

I

S

D

P

P

V

I

Q

K

F

F

F

I

R

R

K

K

A

A

M

K

P

D

A

A

Q

M

L

I

V

T

A

A

F

F

S

V

T

T

S
x
R

S

S

S
|
S

F

S

G

G

T

T

L

L

P

P

A

V

S

T

T

M

R

R

-

V

A

A

V

E

E

E

T

E

M

M

G

G

V

V

S

D

K

K

R

G

Y

I

S

F

A

S

F

F

V

T

M

L

P

P

L

L

G

G

A

A

T

T

M

I

N

N

M
|
M

D

D

G

G

C
x
T

G

-

G

A

I

L

Y

Y

P

Q

A

G

I

V

A

T

A

V

I

L

F

F

I

V

A

A

Q

N

I

A

Y

I

G

G

I

H

P

P

L

L

D

T

T

L

L

G

D

Q

Y

Q

V

L

M

V

I

V

A

V

V

L

T

T

A

A

T

V

V

L

A

A

S

S

V

I

G

G

T
|
T

A

A

G
|
G

V
|
V

P

P

G

G

S

A

A
x
G

M

A

V

I

M

M

L

L

T

A

V

M

T

V

L

L

G

Q

V

S

I

V

G

G

L

L

P

D

L

L

E

T

-

P

-

G

-

S

-

P

-

V

-

A

-

L

G

A

I

Y

A

A

F

M

I

I

A

L

A

G

I

I

D

D

R

A

I

I

I

L

D
|
D

M

M

I

G

R
|
R

T
|
T

A

M

T

V

N
|
N

V

V

T

T

G

G

D

D

M

L

M

A

T

G

A

T

V

V

V

I

I

V

G

A

K

K

S

T

E

E

N

K

E

E

L

L

D

D

V

E

E

S

Q

K

F

W

Y

I

S

S

N

H

binding d-aspartic acid: R263 (≠ S261), S265 (= S263), M299 (= M296), T302 (≠ C299), T340 (= T338), G342 (= G340), V343 (= V341), G347 (≠ A345), D383 (= D374), R386 (= R377), T387 (= T378), N390 (= N381)
binding decyl-beta-d-maltopyranoside: H23 (≠ E31), V212 (≠ L209), A216 (≠ G213)

2nwwA Crystal structure of gltph in complex with tboa (see paper)
39% identity, 93% coverage: 10:396/417 of query aligns to 2:407/407 of 2nwwA

query
sites
2nwwA

P

P

F

V

W

L

Q

Q

K

K

V

I

L

L

A

I

G

G

F

L

I

I

L

L

G

G

A

A

L

I

V

V

G

G

V

L

L

I

L

L

G

G

E

H

-

Y

-

G

-

Y

-

A

-

H

-

A

T

V

A

H

T

T

V

Y

L

V

K

K

P

P

L

F

G

G

D

D

L

L

F

F

I

V

S

R

A

L

I

L

K

K

M

M

L

L

V

V

A

M

P

P

L

I

V

V

F

F

C

A

A

S

I

L

V

V

S

G

I

A

T

A

S

S

L

I

G

-

S

S

Q

P

T

A

N

R

L

L

K

G

R

R

L

V

S

G

L

V

K

K

T

I

L

V

G

V

M

Y

F

Y

M

L

L

L

T

T

G

S

T

A

V

F

A

A

S

V

L

T

I

L

G

G

L

I

A

I

V

M

G

A

S

R

L

L

I

F

D

N

M

P

G

G

G

A

T

G

M

I

Q

H

L

L

A

A

T

V

-

G

T

Q

E

Q

V

F

R

Q

E

P

R

H

N

Q

I

A

P

P

G

P

F

L

A

V

Q

H

V

I

L

L

D

D

M

I

I

V

P

P

V

T

N

N

P

P

F

F

A

G

S

A

L

L

A

A

D

N

G

G

K

Q

V

V

L

L

Q

P

I

T

I

I

V

F

F

F

A

A

A

I

L

I

V

L

G

G

I

I

A

A

I

I

-

T

-

Y

-

L

-

M

-

N

-

S

-

E

-

N

N

E

K

K

V

V

G

R

E

K

K

S

A

A

E

E

P

T

L

L

K

L

R

D

T

A

I

I

E

N

A

G

G

L

A

A

E

E

V

A

M

M

F

Y

Q

K

L

I

T

V

R

N

M

G

V

V

L

M

K

Q

L

Y

T

A

P

P

I

I

G

G

V

V

F

F

G

A

L

L

M

I

A

A

W

Y

V

V

V

M

G

A

E

E

Y

Q

G

G

L

V

S

H

T

V

L

V

L

G

P

E

L

L

G

A

K

K

F

V

I

T

A

A

I

V

Y

Y

I

V

A

G

A

L

L

T

I

L

H

Q

M

I

I

L

F

L

V

V

Y

Y

G

F

G

V

L

L

V

L

K

K

F

I

A

Y

A

-

G

G

L

I

S

D

P

P

V

I

Q

S

F

F

F

I

R

K

K

H

A

A

M

K

P

D

A

A

Q

M

L

L

V

T

A

A

F

F

S

V

T

T

S

R

S

S

S
|
S

F

S

G

G

T

T

L

L

P

P

A

V

S

T

T

M

R

R

A

V

V

A

E

K

T

E

M

M

G

G

V

I

S

S

K

E

R

G

Y

I

S

Y

A

S

F

F

V

T

M

L

P

P

L

L

G

G

A

A

T

T

M

I

N

N

M

M

D

D

G

G

C
x
T

G

-

G

A

I

L

Y

Y

P

Q

A

G

I

V

A

C

A

T

I

F

F

F

I

I

A

A

Q

N

I

A

Y

L

G

G

I

S

P

H

L

L

D

T

T

V

L

G

D

Q

Y

Q

V

L

M

T

I

I

A

V

V

L

T

T

A

A

T

V

V

L

A

A

S

S

V

I

G

G

T

T

A

A

G

G

V

V

P

P

G

G

S
x
A

A
x
G

M

A

V

I

M

M

L

L

T

A

V

M

T

V

L

L

G

H

V

S

I

V

G

G

L

L

P

P

L

L

E

T

-

D

-

P

-

N

-

V

-

A

G

A

I

Y

A

A

F

M

I

I

A

L

A

G

I

I

D

D

R

A

I

I

I

L

D
|
D

M

M

I

G

R
|
R

T
|
T

A

M

T

V

N
|
N

V

V

T

T

G

G

D

D

M

L

M

T

T

G

A

T

V

A

V

I

I

V

G

A

K

K

S

T

E

E

binding (3s)-3-(benzyloxy)-l-aspartic acid: S269 (= S263), T305 (≠ C299), A349 (≠ S344), G350 (≠ A345), D385 (= D374), R388 (= R377), T389 (= T378), N392 (= N381)

6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
39% identity, 94% coverage: 10:399/417 of query aligns to 11:419/419 of 6x15A

query
sites
6x15A

P

P

F

V

W

L

Q

Q

K

K

V

I

L

L

A

I

G

G

F

L

I

I

L

L

G

G

A

A

L

I

V

V

G

G

V

L

L

I

L

L

G

G

E

H

-

Y

-

G

-

Y

-

A

-

H

-

A

T

V

A

H

T

T

V

Y

L

V

K

K

P

P

L
x
F

G

G

D

D

L

L

F

F

I

V

S

R

A

L

I

L

K

C

M

M

L

L

V

V

A

M

P

P

L

I

V

V

F

F

C

A

A

S

I

L

V

V

S

G

I

A

T

A

S

S

L

I

G

-

S

S

Q
x
P

T

A

N

R

L

L

K

G

R

R

L

V

S

G

L

V

K

K

T

I

L

V

G

V

M

Y

F
x
Y

M

L

L
|
L

T
|
T

G
x
S

T

A

V
x
F

A

A

S

V

L

T

I

L

G

G

L

I

A

I

V

M

G

A

S

R

L

L

I

F

D

N

M

P

G

G

G

A

T

G

M

I

Q

H

L

L

A

A

T

V

-

G

T

Q

E

Q

V

F

R

Q

E

P

R

H

N

Q

I

A

P

P

G

P

F
x
L

A

V

Q

H

V
x
I

L

L

D

D

M

I

I

V

P

P

V

T

N

N

P

P

F

F

A

G

S

A

L

L

A

A

D

N

G

G

K

Q

V

V

L

L

Q

P

I

T

I

I

V

F

F

F

A

A

A
x
I

L

I

V

L

G

G

I

I

A

A

I

I

-

T

-
x
Y

-

L

-

M

-

N

-

S

-

E

-

N

N

E

K

K

V

V

G

R

E

K

K

S

A

A

E

E

P

T

L

L

K

L

R

D

T

A

I

I

E

N

A

G

G

L

A

A

E

E

V

A

M

M

F

Y

Q
x
K

L

I

T

V

R

N

M
x
G

V

V

L

M

K

Q

L

Y

T

A

P

P

I
|
I

G

G

V

V

F
|
F

G

A

L

L

M

I

A

A

W

Y

V

V

V

M

G

A

E

E

Y

Q

G

G

L

V

S

H

T

V

L

V

L

G

P

E

L

L

G

A

K

K

F

V

I

T

A

A

I

V

Y

Y

I

V

A

G

A

L

L

T

I

L

H

Q

M

I

I

L

F

L

V

V

Y

Y

G

F

G

V

L
|
L

V

L

K

K

F

I

A

Y

A

-

G

G

L

I

S

D

P

P

V

I

Q

S

F

F

F
x
I

R

K

K

H

A

A

M

K

P

D

A

A

Q
x
M

L

L

V

T

A

A

F

F

S

V

T

T

S

R

S
|
S

F

S

G

G

T

T

L

L

P

P

A

V

S

T

T

M

R

R

A

V

V

A

E

K

T

E

M

M

G

G

V

I

S

S

K

E

R

G

Y

I

S

Y

A

S

F

F

V

T

M

L

P

P

L

L

G
|
G

A

A

T
|
T

M

I

N
|
N

M
|
M

D
|
D

G

G

C
x
T

G

-

G

A

I

L

Y

Y

P

Q

A

G

I

V

A

A

A

T

I

F

F

F

I

I

A

A

Q

N

I

A

Y

L

G

G

I

S

P

H

L

L

D
x
T

T
x
V

L
x
G

D

Q

Y

Q

V

L

M
x
T

I

I

A

V

V
x
L

T

T

A

A

T

V

V

L

A

A

S
|
S

V
x
I

G

G

T
|
T

A

A

G
|
G

V

V

P

P

G

G

S
x
A

A
x
G

M

A

V

I

M

M

L

L

T

C

V

M

T

V

L

L

G

H

V

S

I

V

G

G

L

L

P

P

L

L

E

T

-

D

-

P

-

N

-

V

-

A

G

A

I

Y

A

A

F

M

I

I

A

L

A

G

I

I

D

D

R

A

I

I

I

L

D
|
D

M

M

I

G

R
|
R

T
|
T

A
x
M

T

V

N
|
N

V
|
V

T

T

G

G

D
|
D

M

L

M

T

T

G

A

T

V

A

V

I

I

V

G

A

K

K

S

T

E

E

N

G

E

T

L

L

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: F46 (≠ L39), F46 (≠ L39), P75 (≠ Q69), L91 (= L85), F95 (≠ V89), L130 (≠ F122), I133 (≠ V125), I159 (≠ A151), Y167 (vs. gap), K196 (≠ Q180), G200 (≠ M184), I207 (= I191), F210 (= F194), L250 (= L234), I262 (≠ F247), M269 (≠ Q254), T334 (≠ D320), V335 (≠ T321), G336 (≠ L322), T340 (≠ M326), L343 (≠ V329), M399 (≠ A379)
binding aspartic acid: S277 (= S262), S278 (= S263), T314 (≠ C299), G354 (= G340), A358 (≠ S344), G359 (≠ A345), D394 (= D374), R397 (= R377), T398 (= T378)
binding sodium ion: Y89 (≠ F83), T92 (= T86), S93 (≠ G87), G306 (= G291), T308 (= T293), N310 (= N295), N310 (= N295), M311 (= M296), D312 (= D297), S349 (= S335), I350 (≠ V336), T352 (= T338), N401 (= N381), V402 (= V382), D405 (= D385)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 4, 7

6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
39% identity, 93% coverage: 10:396/417 of query aligns to 8:413/413 of 6x14A

query
sites
6x14A

P

P

F

V

W

L

Q

Q

K

K

V
x
I

L

L

A

I

G

G

F

L

I

I

L

L

G

G

A

A

L

I

V

V

G

G

V

L

L

I

L

L

G

G

E

H

-

Y

-

G

-

Y

-

A

-

H

-

A

T

V

A

H

T

T

V

Y

L

V

K

K

P

P

L

F

G

G

D

D

L

L

F

F

I

V

S

R

A

L

I

L

K

C

M

M

L

L

V

V

A

M

P

P

L

I

V

V

F

F

C

A

A

S

I

L

V

V

S
x
G

I

A

T

A

S

S

L

I

G

-

S

S

Q

P

T

A

N

R

L

L

K

G

R

R

L

V

S

G

L

V

K

K

T

I

L
x
V

G

V

M

Y

F

Y

M

L

L

L

T

T

G

S

T

A

V

F

A

A

S

V

L

T

I

L

G

G

L

I

A

I

V

M

G

A

S

R

L

L

I

F

D

N

M

P

G

G

G

A

T

G

M

I

Q

H

L

L

A

A

T

V

-

G

T

Q

E

Q

V

F

R

Q

E

P

R

H

N

Q

I

A

P

P

G

P

F

L

A

V

Q

H

V

I

L

L

D

D

M

I

I

V

P

P

V

T

N

N

P

P

F

F

A

G

S

A

L

L

A

A

D

N

G

G

K

Q

V

V

L

L

Q

P

I

T

I

I

V

F

F

F

A

A

A

I

L
x
I

V

L

G

G

I

I

A

A

I

I

-

T

-
x
Y

-

L

-

M

-

N

-

S

-

E

-

N

N

E

K

K

V

V

G

R

E

K

K

S

A

A

E

E

P

T

L

L

K

L

R

D

T

A

I

I

E

N

A

G

G

L

A

A

E

E

V

A

M

M

F

Y

Q
x
K

L

I

T

V

R

N

M

G

V

V

L
x
M

K

Q

L

Y

T

A

P

P

I

I

G

G

V

V

F

F

G

A

L

L

M

I

A

A

W

Y

V

V

V

M

G

A

E

E

Y

Q

G

G

L

V

S

H

T

V

L

V

L

G

P

E

L

L

G

A

K

K

F

V

I

T

A

A

I

V

Y

Y

I

V

A

G

A

L

L

T

I

L

H

Q

M

I

I

L

F

L

V

V

Y

Y

G

F

G

V

L

L

V

L

K

K

F

I

A

Y

A

-

G

G

L

I

S

D

P

P

V

I

Q

S

F

F

F

I

R

K

K

H

A

A

M

K

P

D

A

A

Q

M

L

L

V

T

A

A

F

F

S

V

T

T

S

R

S

S

S
|
S

F

S

G

G

T

T

L

L

P

P

A

V

S

T

T

M

R

R

A

V

V

A

E

K

T

E

M

M

G

G

V

I

S

S

K

E

R

G

Y

I

S

Y

A

S

F

F

V

T

M

L

P

P

L

L

G

G

A

A

T
|
T

M
x
I

N

N

M

M

D

D

G

G

C
x
T

G

-

G

A

I

L

Y

Y

P

Q

A

G

I

V

A

A

A

T

I

F

F

F

I

I

A

A

Q

N

I

A

Y

L

G

G

I

S

P

H

L

L

D

T

T

V

L

G

D

Q

Y

Q

V

L

M

T

I

I

A

V

V

L

T

T

A

A

T

V

V

L

A

A

S

S

V
x
I

G

G

T

T

A

A

G

G

V

V

P

P

G

G

S

A

A
x
G

M

A

V

I

M

M

L

L

T

C

V

M

T

V

L

L

G

H

V

S

I

V

G

G

L

L

P

P

L

L

E

T

-

D

-

P

-

N

-

V

-

A

G

A

I

Y

A

A

F

M

I

I

A
x
L

A

G

I

I

D

D

R

A

I

I

I

L

D
|
D

M

M

I

G

R
|
R

T

T

A

M

T

V

N

N

V

V

T

T

G

G

D

D

M

L

M

T

T

G

A

T

V

A

V

I

I

V

G

A

K

K

S

T

E

E

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: G66 (≠ S62), V83 (≠ L80), I157 (≠ L152), Y164 (vs. gap), K193 (≠ Q180), T305 (= T293), I306 (≠ M294), I347 (≠ V336)
binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I13 (≠ V15), M199 (≠ L186), S275 (= S263), T311 (≠ C299), G356 (≠ A345), L384 (≠ A367), D391 (= D374), R394 (= R377)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 4

6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
39% identity, 93% coverage: 10:396/417 of query aligns to 3:408/408 of 6bauA

query
sites
6bauA

P

P

F

V

W

L

Q

Q

K

K

V

I

L

L

A

I

G

G

F

L

I

I

L

L

G

G

A

A

L

I

V

V

G

G

V

L

L

I

L

L

G

G

E

H

-

Y

-

G

-

Y

-

A

-

D

-

A

T

V

A

K

T

T

V

Y

L

V

K

K

P

P

L

F

G

G

D

D

L

L

F

F

I

V

S

R

A

L

I

L

K

K

M

M

L

L

V

V

A

M

P

P

L

I

V

V

F

F

C

A

A

S

I

L

V

V

S

G

I

A

T

A

S

S

L

I

G

-

S

S

Q

P

T

A

N

R

L

L

K

G

R

R

L

V

S

G

L

V

K

K

T

I

L

V

G

V

M

Y

F

Y

M

L

L

L

T

T

G

S

T

A

V

F

A

A

S

V

L

T

I

L

G

G

L

I

A

I

V

M

G

A

S

R

L

L

I

F

D

N

M

P

G

G

G

A

T

G

M

I

Q

H

L

L

A

A

T

V

-

G

T

Q

E

Q

V

F

R

Q

E

P

R

K

N

Q

I

A

P

P

G

P

F

L

A

V

Q

K

V

I

L

L

D

D

M

I

I

V

P

P

V

T

N

N

P

P

F

F

A

G

S

A

L

L

A

A

D

N

G

G

K

Q

V

V

L

L

Q

P

I

T

I

I

V

F

F

F

A

A

A

I

L

I

V

L

G

G

I

I

A

A

I

I

-

T

-

Y

-

L

-

M

-

N

-

S

-

E

-

N

N

E

K

K

V

V

G

R

E

K

K

S

A

A

E

E

P

T

L

L

K

L

R

D

T

A

I

I

E

N

A

G

G

L

A

A

E

E

V

A

M

M

F

Y

Q

K

L

I

T

V

R

N

M

G

V

V

L

M

K

Q

L

Y

T

A

P

P

I

I

G

G

V

V

F

F

G

A

L

L

M

I

A

A

W

Y

V

V

V

M

G

A

E

E

Y

Q

G

G

L

V

S

K

T

V

L

V

L

G

P

E

L

L

G

A

K

K

F

V

I

T

A

A

I

V

Y

Y

I

V

A

G

A

L

L

T

I

L

H

Q

M

I

I

L

F

L

V

V

Y

Y

G

F

G

V

L

L

V

L

K

K

F

I

A

Y

A

-

G

G

L

I

S

D

P

P

V

I

Q

S

F

F

F

I

R

K

K

K

A

A

M

K

P

D

A

A

Q

M

L

L

V

T

A

A

F

F

S

V

T

T

S

R

S

S

S
|
S

F

S

G

G

T

T

L

L

P

P

A

V

S

T

T

M

R

R

A

V

V

A

E

K

T

E

M

M

G

G

V

I

S

S

K

E

R

G

Y

I

S

Y

A

S

F

F

V

T

M

L

P

P

L

L

G

G

A

A

T

T

M

I

N

N

M
|
M

D

D

G

G

C
x
T

G

-

G

A

I

L

Y

Y

P

Q

A

G

I

V

A

C

A

T

I

F

F

F

I

I

A

A

Q

N

I

A

Y

L

G

G

I

S

P

H

L

L

D

T

T

V

L

G

D

Q

Y

Q

V

L

M

T

I

I

A

V

V

L

T

T

A

A

T

V

V

L

A

A

S

S

V

I

G

G

T

T

A
|
A

G
|
G

V
|
V

P

P

G

G

S

A

A
x
G

M

A

V

I

M

M

L

L

T

A

V

M

T

V

L

L

G

E

V

S

I

V

G

G

L

L

P

P

L

L

E

T

-

D

-

P

-

N

-

V

-

A

G

A

I

Y

A

A

F

M

I

I

A

L

A

G

I

I

D

D

R

A

I

I

I

L

D
|
D

M

M

I

G

R
x
C

T
|
T

A

M

T

V

N
|
N

V

V

T

T

G

G

D

D

M

L

M

T

T

G

A

T

V

A

V

I

I

V

G

A

K

K

S

T

E

E

binding cysteine: S270 (= S263), M303 (= M296), T306 (≠ C299), A345 (= A339), G346 (= G340), V347 (= V341), G351 (≠ A345), D386 (= D374), C389 (≠ R377), T390 (= T378), N393 (= N381)

6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
39% identity, 93% coverage: 10:396/417 of query aligns to 3:408/409 of 6bavA

query
sites
6bavA

P

P

F

V

W

L

Q

Q

K

K

V

I

L

L

A

I

G

G

F

L

I

I

L

L

G

G

A

A

L

I

V

V

G

G

V

L

L

I

L

L

G

G

E

H

-

Y

-

G

-

Y

-

A

-

D

-

A

T

V

A

K

T

T

V

Y

L

V

K

K

P

P

L

F

G

G

D

D

L

L

F

F

I

V

S

R

A

L

I

L

K

K

M

M

L

L

V

V

A

M

P

P

L

I

V

V

F

F

C

A

A

S

I

L

V

V

S

G

I

A

T

A

S

S

L

I

G

-

S

S

Q

P

T

A

N

R

L

L

K

G

R

R

L

V

S

G

L

V

K

K

T

I

L

V

G

V

M

Y

F

Y

M

L

L

L

T

T

G

S

T

A

V

F

A

A

S

V

L

T

I

L

G

G

L

I

A

I

V

M

G

A

S

R

L

L

I

F

D

N

M

P

G

G

G

A

T

G

M

I

Q

H

L

L

A

A

T

V

-

G

T

Q

E

Q

V

F

R

Q

E

P

R

K

N

Q

I

A

P

P

G

P

F

L

A

V

Q

K

V

I

L

L

D

D

M

I

I

V

P

P

V

T

N

N

P

P

F

F

A

G

S

A

L

L

A

A

D

N

G

G

K

Q

V

V

L

L

Q

P

I

T

I

I

V

F

F

F

A

A

A

I

L

I

V

L

G

G

I

I

A

A

I

I

-

T

-

Y

-

L

-

M

-

N

-

S

-

E

-

N

N

E

K

K

V

V

G

R

E

K

K

S

A

A

E

E

P

T

L

L

K

L

R

D

T

A

I

I

E

N

A

G

G

L

A

A

E

E

V

A

M

M

F

Y

Q

K

L

I

T

V

R

N

M

G

V

V

L

M

K

Q

L

Y

T

A

P

P

I

I

G

G

V

V

F

F

G

A

L

L

M

I

A

A

W

Y

V

V

V

M

G

A

E

E

Y

Q

G

G

L

V

S

K

T

V

L

V

L

G

P

E

L

L

G

A

K

K

F

V

I

T

A

A

I

V

Y

Y

I

V

A

G

A

L

L

T

I

L

H

Q

M

I

I

L

F

L

V

V

Y

Y

G

F

G

V

L

L

V

L

K

K

F

I

A

Y

A

-

G

G

L

I

S

D

P

P

V

I

Q

S

F

F

F

I

R

K

K

K

A

A

M

K

P

D

A

A

Q

M

L

L

V

T

A

A

F

F

S

V

T

T

S
x
R

S

S

S
|
S

F

S

G

G

T

T

L

L

P

P

A

V

S

T

T

M

R

R

A

V

V

A

E

K

T

E

M

M

G

G

V

I

S

S

K

E

R

G

Y

I

S

Y

A

S

F

F

V

T

M

L

P

P

L

L

G

G

A

A

T

T

M

I

N

N

M

M

D

D

G

G