SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 7026099 FitnessBrowser__ANA3:7026099 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
36% identity, 93% coverage: 20:272/272 of query aligns to 12:263/265 of P07821

query
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P07821

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K50 (= K58) mutation to Q: Lack of activity.
D172 (= D181) mutation to E: Lack of activity.
E173 (= E182) mutation to A: Lack of activity.

1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
32% identity, 78% coverage: 26:238/272 of query aligns to 10:226/232 of 1f3oA

query
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binding adenosine-5'-diphosphate: Y11 (= Y27), G41 (= G55), G43 (= G57), K44 (= K58), S45 (= S59), T46 (= T60)

5x40A Structure of a cbio dimer bound with amppcp (see paper)
34% identity, 83% coverage: 20:246/272 of query aligns to 5:229/280 of 5x40A

query
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binding phosphomethylphosphonic acid adenylate ester: F14 (≠ I29), V18 (≠ K32), A20 (≠ V34), N40 (= N54), G41 (= G55), G43 (= G57), K44 (= K58), S45 (= S59), T46 (= T60), Q88 (≠ P99), H139 (≠ P150), M140 (≠ T151), L141 (= L152), S142 (= S153), G144 (= G155), Q145 (≠ E156), Q166 (≠ E182), H198 (= H215)
binding magnesium ion: S45 (= S59), Q88 (≠ P99)

1l2tA Dimeric structure of mj0796, a bacterial abc transporter cassette (see paper)
32% identity, 78% coverage: 26:238/272 of query aligns to 10:226/230 of 1l2tA

query
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1l2tA

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binding adenosine-5'-triphosphate: Y11 (= Y27), S40 (≠ N54), G41 (= G55), S42 (≠ A56), G43 (= G57), K44 (= K58), S45 (= S59), T46 (= T60), F138 (≠ A145), Q145 (vs. gap), S147 (= S153), G149 (= G155), Q150 (≠ E156), H204 (= H215)

3nhaA Nucleotide binding domain of human abcb6 (adp mg bound structure) (see paper)
31% identity, 86% coverage: 14:246/272 of query aligns to 35:263/278 of 3nhaA

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binding adenosine-5'-diphosphate: Y50 (≠ I29), T55 (≠ V34), G76 (= G55), G78 (= G57), K79 (= K58), S80 (= S59), T81 (= T60)

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
30% identity, 82% coverage: 18:239/272 of query aligns to 2:216/369 of P19566

query
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P19566

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N

L

V

S

T

Y

K

A

A

I

W

G

G

D

D

K

V

A

V

V

V

L

S

K

K

D

D

I

I

S

N

V

L

Q

D

F

I

Q

H

P

D

G

G

S

E

F

F

T

V

A

V

L

F

L

V

G

G

P

P

N

S

G

G

A

C

G

G

K

K

S

S

T

T

L

L

K

R

A

M

L

I

C

A

Q

G

E

L

I

E

P

T

A

I

A

T

R

S

D

G

S

D

I

L

R

F

L

I

G

G

H

E

C

T

P

R

L

M

V

N

D

D

W

I

P

P

R

P

A

A

E

E

L

-

A

-

K

R

S

G

L

V

A

G

V

M

L

V

P

F

Q

Q

H

S

A

Y

S

A

L
|
L

T

Y

F

P

P

H

F

L

K

S

V

V

H

A

E

E

V

N

V

M

A

S

M

F

G

G

L

L

Y

K

P

L

L

A

T

G

L

A

S

K

Q

K

K

E

E

V

G

M

Q

N

Q

Q

L

R

V

V

T

N

K

Q

W

V

L

A

T

E

E

V

V

L

E

Q

I

L

V

A

H

H

L

L

A

L

Q

E

R

R

S

K

Y

P

P

K

T

A

L

L

S

S

G

G

E

Q

K

R

Q

Q

R

R

V

V

Q

A

L

I

A

G

R

R

V

T

L

L

T

V

Q

-

L

-

S

-

Q

-

S

-

P

A

F

E

P

P

P

R

I

V

L

F

L

L

D

D

E

E

P
|
P

T

L

S

S

A

N

L

L

D
|
D

L

A

A

A

Q

L

Q

R

H

V

K

Q

V

M

L

R

A

I

L

E

A

I

K

S

N

R

L

L

A

H

H

K

K

R

H

L

A

G

Y

R

T

T

V

M

I

I

V

Y

V

V

L

T

H

H

D

D

L

Q

N

V

Q

E

A

A

A

M

R

T

Y

L

S

A

D

D

R

K

V

I

I

V

V

V

L

L

K

D

H

A

G

G

E

R

I

V

V

A

S

Q

E

V

G

G

L86 (= L104) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P183) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D188) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
31% identity, 81% coverage: 22:240/272 of query aligns to 4:219/240 of 4ymuJ

query
sites
4ymuJ

V

V

D

N

R

D

L

V

S

Y

Y

K

A

N

I
x
F

G

G

D

S

K

L

A

E

V
|
V

L

L

K

K

D

G

I

V

S

T

V

L

Q

K

F

V

Q

N

P

K

G

G

S

E

F

V

T

V

A

V

L

I

G

G

P

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

K

R

A

C

-

I

-

N

L

L

C

L

Q

E

E

E

I

P

P

T

A

K

A

G

R

E

-

V

-

F

-

I

D

D

S

G

I

V

R

K

L

I

G

N

H

N

C

G

P

K

L

V

V

-

D

-

W

-

P

N

R

I

A

N

E

K

L

V

A

R

K

Q

S

K

L

V

A

G

V

M

L

V

P

F

Q

Q

H

H

A

F

S

N

L

L

T

-

F

F

P

P

F

-

K

H

V

L

H

T

E

A

V

I

V

E

A

N

M

I

G

T

L

L

Y

A

P

P

L

V

-

K

-

V

-

K

T

K

L

M

S

N

Q

K

K

K

E

E

G

A

Q

E

L

L

V

A

T

V

K

D

W

L

L

L

T

A

E

K

V

V

E

G

I

L

V

L

H

D

L

-

A

K

Q

K

R

D

S

Q

Y

Y

P

P

T

I

-

K

L

L

S

S

G

G

E

Q

K

K

Q

Q

R

R

V

L

Q

A

L

I

A

A

R

R

V

A

L

L

T

A

Q

M

L

Q

S

-

Q

-

S

-

P

-

F

-

P

P

P

E

I

V

L

M

L

L

L

F

D

D

E
|
E

P

P

T

T

S

S

A

A

L

L

D

D

L

P

A

E

Q

M

Q

V

H

K

K

E

V

V

L

L

A

N

L

V

A

M

K

K

N

Q

L

L

A

A

H

N

K

-

H

E

A

G

Y

M

T

T

V

M

I

V

V

V

L

T

H
|
H

D

E

L

M

N

G

Q

F

A

A

A

R

R

E

Y

V

S

G

D

D

R

R

V

V

I

I

V

F

L

M

K

D

H

D

G

G

E

V

I

I

V

V

S

E

E

E

G

G

S

T

binding adenosine-5'-triphosphate: F11 (≠ I29), V16 (= V34), S36 (≠ N54), G37 (= G55), S38 (≠ A56), G39 (= G57), K40 (= K58), S41 (= S59), T42 (= T60), E162 (= E182), H194 (= H215)
binding magnesium ion: S41 (= S59), E162 (= E182)

8bmsA Cryo-em structure of the mutant solitary ecf module 2eq in msp2n2 lipid nanodiscs in the atpase closed and atp-bound conformation (see paper)
29% identity, 81% coverage: 20:239/272 of query aligns to 5:222/278 of 8bmsA

query
sites
8bmsA

L

I

S

S

V

F

D

D

R

H

L

V

S

T

Y
x
F

A

T

I

D

G

S

D

P

K

R

A

P

V

A

L

L

K

S

D

D

I

L

S

S

V

F

Q

A

F

I

Q

E

P

R

G

G

S

S

F

W

T

T

A

A

L

L

L

I

G

G

P

H

N
|
N

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

V

L

S

K

K

A

L

L

I

-

N

-

G

-

L

-

A

-

P

-

D

C

L

Q

D

E

K

I

S

P

S

A

I

A

T

R

V

D

D

S

G

I

V

R

K

L

L

G

G

H

A

C

D

P

T

L

V

V

-

D

-

W

W

P

-

R

-

A

-

E

E

L

V

A

R

K

E

S

K

L

V

A

G

V

I

L

V

P

F

Q
|
Q

H

N

A

P

S

D

L

N

T

Q

F

F

-

V

P

G

F

A

K

T

V

V

H

S

E

D

V

D

V

V

A

A

M

F

G

G

L

L

Y

E

P

N

L

R

T

A

L

V

S

P

Q

R

K

P

E

E

G

M

Q

L

Q

K

L

I

V

V

T

A

K

Q

W

A

L

V

T

A

E

D

V

V

E

G

I

M

V

A

H

D

L
x
Y

A

A

Q

D

R

S

S

E

Y

P

P

S

T
x
N

L

L

S
|
S

G
|
G

E
x
Q

K

K

Q

Q

R

R

V

V

Q

A

L

I

A

A

R

G

V

I

L

L

T

-

Q

-

L

-

S

-

Q

-

S

A

P

V

F

K

P

P

P

Q

I

V

L

I

L

L

D

D

E
x
Q

P

S

T

T

S

S

A

M

L

L

D

D

L

P

A

E

Q

G

Q

K

H

E

K

Q

V

I

L

L

A

D

L

L

A

V

K

R

N

K

L

I

A

K

H

E

K

D

H

N

A

N

Y

L

T

T

V

V

I

I

V

S

V

I

L

T

H
|
H

D

D

L

L

N

E

Q

E

A

A

R

-

Y

G

S

A

D

D

R

Q

V

V

I

L

V

V

L

L

K

D

H

D

G

G

E

Q

I

L

V

L

S

D

E

Q

G

G

binding adenosine-5'-triphosphate: F12 (≠ Y27), N39 (= N54), G40 (= G55), S41 (≠ A56), G42 (= G57), K43 (= K58), S44 (= S59), T45 (= T60), Q88 (= Q100), Y133 (≠ L144), N140 (≠ T151), S142 (= S153), G143 (= G154), G144 (= G155), Q145 (≠ E156), Q166 (≠ E182), H199 (= H215)
binding magnesium ion: S44 (= S59), Q88 (= Q100)

3nh9A Nucleotide binding domain of human abcb6 (atp bound structure) (see paper)
31% identity, 86% coverage: 14:246/272 of query aligns to 29:258/273 of 3nh9A

query
sites
3nh9A

H

R

F

F

E

Q

R

K

A

G

Q

R

L

I

S

E

V

F

D

E

R

N

L

V

S

H

Y

F

A

S

I
x
Y

G

A

D

D

-

G

K

R

A

E

V

T

L

L

K

Q

D

D

I

V

S

S

V

F

Q

T

F

V

Q

M

P

P

G

G

S

Q

F

T

T

L

A

A

L

L

L

V

G

G

P

P

N
x
S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

I

L

L

K
x
R

A

L

L

L

C

F

Q

R

E

F

I

Y

P

D

A

I

A

S

R

S

D

G

S

C

I

I

R

R

L

I

G

D

H

G

C

Q

P

D

L

I

V

S

D

Q

W

V

P

T

R

Q

A

A

E

S

L

L

A

R

K

S

S

H

L

I

A

G

V

V

L

V

P

P

Q

Q

H

D

A

T

S

V

L

L

T

-

F

F

P

N

F

D

K

T

V

I

H

A

E

D

V

N

V

I

A

R

M

Y

G

G

L

-

Y

-

P

-

L

-

T

-

L

-

S

-

Q

-

K

-

E

-

G

-

Q

-

L

R

V

V

T

T

K

A

W

G

L

N

T

D

E

E

V

V

E

E

I

A

-

A

-

Q

-

A

-

G

V

I

H

H

L

D

A

A

Q

I

R

M

S

A

Y

F

P

P

-

E

-

G

-

Y

-

R

-

T

-

Q

-

V

-

G

-

E

-

R

-

G

-

L

T

K

L

L

S

S

G

G

E

E

K

K

Q

Q

R

R

V

V

Q

A

L

I

A

A

R

R

V

T

L

I

T

L

Q

K

L

A

S

-

Q

-

S

-

P

-

F

-

P

P

P

G

I

I

L

I

L

L

D

D

E

E

P

A

T

T

S

S

A

A

L

L

D

D

L

T

A

S

Q

N

Q

E

H

R

K

A

V

I

L

Q

A

A

-

S

L

L

A

A

K

K

N

V

L

C

A

A

H

N

K

R

H

-

A

-

Y

-

T

T

V

T

I

I

V

V

L

A

H

H

D

R

L

L

N

S

Q

T

A

V

R

N

Y

-

S

A

D

D

R

Q

V

I

I

L

V

V

L

I

K

K

H

D

G

G

E

C

I

I

V

V

S

E

E

R

G

G

S

R

A

H

S

E

K

A

A

L

L

L

S

S

binding adenosine-5'-triphosphate: Y44 (≠ I29), S70 (≠ N54), G71 (= G55), A72 (= A56), G73 (= G57), K74 (= K58), S75 (= S59), T76 (= T60), R79 (≠ K63)

5d3mA Folate ecf transporter: amppnp bound state (see paper)
31% identity, 81% coverage: 20:239/272 of query aligns to 6:225/280 of 5d3mA

query
sites
5d3mA

L

I

S

S

V

F

D

D

R

H

L

V

S

T

Y
x
F

A

T

I

Y

G

P

D

D

-

S

-

P

K

R

A

P

V

A

L

L

K

S

D

D

I

L

S

S

V

F

Q

A

F

I

Q

E

P

R

G

G

S

S

F

W

T

T

A

A

L

L

L

I

G

G

P

H

N
|
N

G
|
G

A

S

G

G

K
|
K

S
|
S

T

T

-

V

-

S

-

K

L

L

L

I

K

N

A

G

L

L

C

L

Q

A

E

P

I

D

P

D

A

L

A

D

R

K

D

S

S

S

I

I

R

T

L

V

G

D

H

G

C

V

P

K

L

L

V

G

D

A

W

D

P

T

R

V

A

W

E

E

L

V

A

R

K

E

S

K

L

V

A

G

V

I

L

V

P

F

Q

Q

H

N

A

P

S

D

L

N

T

Q

F

F

-

V

P

G

F

A

K

T

V

V

H

S

E

D

V

D

V

V

A

A

M

F

G

G

L

L

Y

E

P

N

L

R

T

A

L

V

S

P

Q

R

K

P

E

E

G

M

Q

L

Q

K

L

I

V

V

T

A

K

Q

W

A

L

V

T

A

E

D

V

V

E

G

I

M

V

A

H

D

L

Y

A

A

Q

D

R

S

S

E

Y

P

P

S

T

N

L

L

S

S

G

G

E

Q

K

K

Q

Q

R

R

V

V

Q

A

L

I

A

A

R

G

V

I

L

L

T

-

Q

-

L

-

S

-

Q

-

S

A

P

V

F

K

P

P

P

Q

I

V

L

I

L

L

D

D

E

E

P

S

T

T

S

S

A

M

L

L

D

D

L

P

A

E

Q

G

Q

K

H

E

K

Q

V

I

L

L

A

D

L

L

A

V

K

R

N

K

L

I

A

K

H

E

K

D

H

N

A

N

Y

L

T

T

V

V

I

I

V

S

V

I

L

T

H

H

D

D

L

L

N

E

Q

E

A

A

R

G

Y

-

S

A

D

D

R

Q

V

V

I

L

V

V

L

L

K

D

H

D

G

G

E

Q

I

L

V

L

S

D

E

Q

G

G

binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ Y27), N42 (= N54), G43 (= G55), K46 (= K58), S47 (= S59)

7dnyB Cryo-em structure of the human abcb6 (coproporphyrin iii-bound) (see paper)
31% identity, 86% coverage: 14:246/272 of query aligns to 331:560/575 of 7dnyB

query
sites
7dnyB

H

R

F

F

E

Q

R

K

A

G

Q

R

L

I

S

E

V

F

D

E

R

N

L

V

S

H

Y

F

A

S

I

Y

G

A

D

D

-

G

K

R

A

E

V

T

L

L

K

Q

D

D

I

V

S

S

V

F

Q

T

F

V

Q

M

P

P

G

G

S

Q

F

T

T

L

A

A

L

L

L

V

G

G

P

P

N

S

G

G

A

A

G

G

K

K

S

S

T

T

L

I

L

L

K

R

A

L

L

L

C

F

Q

R

E

F

I

Y

P

D

A

I

A

S

R

S

D

G

S

C

I

I

R

R

L

I

G

D

H

G

C

Q

P

D

L

I

V

S

D

Q

W

V

P

T

R

Q

A

A

E

S

L

L

A

R

K

S

S

H

L

I

A

G

V

V

L

V

P

P

Q

Q

H

D

A

T

S

V

L

L

T

-

F

F

P

N

F

D

K

T

V

I

H

A

E

D

V

N

V

I

A

R

M

Y

G

G

L

-

Y

-

P

-

L

-

T

-

L

-

S

-

Q

-

K

-

E

-

G

-

Q

-

L

R

V

V

T

T

K

A

W

G

L

N

T

D

E

E

V

V

E

E

I

A

-

A

-

Q

-

A

-

G

V

I

H

H

L

D

A

A

Q

I

R

M

S

A

Y

F

P

P

-

E

-

G

-

Y

-

R

-

T

-

Q

-

V

-

G

-

E

-

R

-

G

-

L

T

K

L

L

S

S

G

G

E

E

K

K

Q

Q

R

R

V

V

Q

A

L

I

A

A

R

R

V

T

L

I

T

L

Q

K

L

A

S

-

Q

-

S

-

P

-

F

-

P

P

P

G

I

I

L

I

L

L

D

D

E

E

P

A

T

T

S

S

A

A

L

L

D

D

L

T

A

S

Q

N

Q

E

H

R

K

A

V

I

L

Q

A

A

-

S

L

L

A

A

K

K

N

V

L

C

A

A

H

N

K

R

H

-

A

-

Y

-

T

T

V

T

I

I

V

V

L

A

H

H

D

R

L

L

N

S

Q

T

A

V

R

N

Y

-

S

A

D

D

R

Q

V

I

I

L

V

V

L

I

K

K

H

D

G

G

E

C

I

I

V

V

S

E

E

R

G

G

S

R

A

H

S

E

K

A

A

L

L

L

S

S

Sites not aligning to the query:

binding coproporphyrin III: 293, 297
binding cholesterol hemisuccinate: 19, 23, 24, 31, 34, 51, 54, 58, 151, 155

8k7bA Post-occluded structure of human abcb6 w546a mutant (adp/vo4-bound) (see paper)
31% identity, 86% coverage: 14:246/272 of query aligns to 347:576/590 of 8k7bA

query
sites
8k7bA

H

R

F

F

E

Q

R

K

A

G

Q

R

L

I

S

E

V

F

D

E

R

N

L

V

S

H

Y

F

A

S

I
x
Y

G

A

D

D

-

G

K

R

A

E

V

T

L

L

K

Q

D

D

I

V

S

S

V

F

Q

T

F

V

Q

M

P

P

G

G

S

Q

F

T

T

L

A

A

L

L

L

V

G

G

P

P

N

S

G
|
G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

L

I

L

L

K

R

A

L

L

L

C

F

Q

R

E

F

I

Y

P

D

A

I

A

S

R

S

D

G

S

C

I

I

R

R

L

I

G

D

H

G

C

Q

P

D

L

I

V

S

D

Q

W

V

P

T

R

Q

A

A

E

S

L

L

A

R

K

S

S

H

L

I

A

G

V

V

L

V

P

P

Q
|
Q

H

D

A

T

S

V

L

L

T

-

F

F

P

N

F

D

K

T

V

I

H

A

E

D

V

N

V

I

A

R

M

Y

G

G

L

-

Y

-

P

-

L

-

T

-

L

-

S

-

Q

-

K

-

E

-

G

-

Q

-

L

R

V

V

T

T

K

A

W

G

L

N

T

D

E

E

V

V

E

E

I

A

-

A

-

Q

-

A

-

G

V

I

H

H

L

D

A

A

Q

I

R

M

S

A

Y

F

P

P

-

E

-

G

-

Y

-

R

-

T

-

Q

-

V

-

G

-

E

-

R

-

G

-

L

T
x
K

L
|
L

S
|
S

G
|
G

G

G

E

E

K

K

Q

Q

R

R

V

V

Q

A

L

I

A

A

R

R

V

T

L

I

T

L

Q

K

L

A

S

-

Q

-

S

-

P

-

F

-

P

P

P

G

I

I

L

I

L

L

D

D

E

E

P

A

T

T

S

S

A

A

L

L

D

D

L

T

A

S

Q

N

Q

E

H

R

K

A

V

I

L

Q

A

A

-

S

L

L

A

A

K

K

N

V

L

C

A

A

H

N

K

R

H

-

A

-

Y

-

T

T

V

T

I

I

V

V

L

A

H
|
H

D

R

L

L

N

S

Q

T

A

V

R

N

Y

-

S

A

D

D

R

Q

V

I

I

L

V

V

L

I

K

K

H

D

G

G

E

C

I

I

V

V

S

E

E

R

G

G

S

R

A

H

S

E

K

A

A

L

L

L

S

S

binding adp orthovanadate: Y362 (≠ I29), G389 (= G55), G391 (= G57), K392 (= K58), S393 (= S59), T394 (= T60), K489 (≠ T151), L490 (= L152), S491 (= S153), G492 (= G154), H546 (= H215)
binding magnesium ion: S393 (= S59), Q434 (= Q100)

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
29% identity, 82% coverage: 18:239/272 of query aligns to 1:215/374 of 2awnB

query
sites
2awnB

A

A

Q

S

L

V

S

Q

V

L

D

Q

R

N

L

V

S

T

Y

K

A

A

I
x
W

G

G

D

E

K

V

A

V

V
|
V

L

S

K

K

D

D

I

I

S

N

V

L

Q

D

F

I

Q

H

P

E

G

G

S

E

F

F

T

V

A

V

L

F

L

V

G

G

P

P

N
x
S

G
|
G

A

C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

K

R

A

M

L

I

C

A

Q

G

E

L

I

E

P

T

A

I

A

T

R

S

D

G

S

D

I

L

R

F

L

I

G

G

H

E

C

K

P

R

L

M

V

N

D

D

W

T

P

P

R

P

A

A

E

E

L

-

A

-

K

R

S

G

L

V

A

G

V

M

L

V

P

F

Q

Q

H

S

A

Y

S

A

L

L

T

Y

F

P

P

H

F

L

K

S

V

V

H

A

E

E

V

N

V

M

A

S

M

F

G

G

L

L

Y

K

P

L

L

A

T

G

L

A

S

K

Q

K

K

E

E

V

G

I

Q

N

Q

Q

L

R

V

V

T

N

K

Q

W

V

L

A

T

E

E

V

V

L

E

Q

I

L

V

A

H

H

L

L

A

L

Q

D

R

R

S

K

Y

P

P

K

T

A

L

L

S

S

G

G

E

Q

K

R

Q

Q

R

R

V

V

Q

A

L

I

A

G

R

R

V

T

L

L

T

V

Q

-

L

-

S

-

Q

-

S

-

P

A

F

E

P

P

P

S

I

V

L

F

L

L

D

D

E

E

P

P

T

L

S

S

A

N

L

L

D

D

L

A

A

A

Q

L

Q

R

H

V

K

Q

V

M

L

R

A

I

L

E

A

I

K

S

N

R

L

L

A

H

H

K

K

R

H

L

A

G

Y

R

T

T

V

M

I

I

V

Y

V

V

L

T

H

H

D

D

L

Q

N

V

Q

E

A

A

A

M

R

T

Y

L

S

A

D

D

R

K

V

I

I

V

V

V

L

L

K

D

H

A

G

G

E

R

I

V

V

A

S

Q

E

V

G

G

binding adenosine-5'-diphosphate: W12 (≠ I29), V17 (= V34), S37 (≠ N54), G38 (= G55), G40 (= G57), K41 (= K58), S42 (= S59), T43 (= T60)

7dnzB Cryo-em structure of the human abcb6 (hemin and gsh-bound) (see paper)
31% identity, 86% coverage: 14:246/272 of query aligns to 349:578/591 of 7dnzB

query
sites
7dnzB

H

R

F

F

E

Q

R

K

A

G

Q

R

L

I

S

E

V

F

D

E

R

N

L

V

S

H

Y

F

A

S

I

Y

G

A

D

D

-

G

K

R

A

E

V

T

L

L

K

Q

D

D

I

V

S

S

V

F

Q

T

F

V

Q

M

P

P

G

G

S

Q

F

T

T

L

A

A

L

L

L

V

G

G

P

P

N

S

G

G

A

A

G

G

K

K

S

S

T

T

L

I

L

L

K

R

A

L

L

L

C

F

Q

R

E

F

I

Y

P

D

A

I

A

S

R

S

D

G

S

C

I

I

R

R

L

I

G

D

H

G

C

Q

P

D

L

I

V

S

D

Q

W

V

P

T

R

Q

A

A

E

S

L

L

A

R

K

S

S

H

L

I

A

G

V

V

L

V

P

P

Q

Q

H

D

A

T

S

V

L

L

T

-

F

F

P

N

F

D

K

T

V

I

H

A

E

D

V

N

V

I

A

R

M

Y

G

G

L

-

Y

-

P

-

L

-

T

-

L

-

S

-

Q

-

K

-

E

-

G

-

Q

-

L

R

V

V

T

T

K

A

W

G

L

N

T

D

E

E

V

V

E

E

I

A

-

A

-

Q

-

A

-

G

V

I

H

H

L

D

A

A

Q

I

R

M

S

A

Y

F

P

P

-

E

-

G

-

Y

-

R

-

T

-

Q

-

V

-

G

-

E

-

R

-

G

-

L

T

K

L

L

S

S

G

G

E

E

K

K

Q

Q

R

R

V

V

Q

A

L

I

A

A

R

R

V

T

L

I

T

L

Q

K

L

A

S

-

Q

-

S

-

P

-

F

-

P

P

P

G

I

I

L

I

L

L

D

D

E

E

P

A

T

T

S

S

A

A

L

L

D

D

L

T

A

S

Q

N

Q

E

H

R

K

A

V

I

L

Q

A

A

-

S

L

L

A

A

K

K

N

V

L

C

A

A

H

N

K

R

H

-

A

-

Y

-

T

T

V

T

I

I

V

V

L

A

H

H

D

R

L

L

N

S

Q

T

A

V

R

N

Y

-

S

A

D

D

R

Q

V

I

I

L

V

V

L

I

K

K

H

D

G

G

E

C

I

I

V

V

S

E

E

R

G

G

S

R

A

H

S

E

K

A

A

L

L

L

S

S

Sites not aligning to the query:

binding glutathione: 111, 135, 139, 204, 310, 314, 317
binding protoporphyrin ix containing fe: 85, 311
binding cholesterol hemisuccinate: 37, 42, 177, 181, 184, 280

7dnzA Cryo-em structure of the human abcb6 (hemin and gsh-bound) (see paper)
31% identity, 86% coverage: 14:246/272 of query aligns to 349:578/591 of 7dnzA

query
sites
7dnzA

H

R

F

F

E

Q

R

K

A

G

Q

R

L

I

S

E

V

F

D

E

R

N

L

V

S

H

Y

F

A

S

I

Y

G

A

D

D

-

G

K

R

A

E

V

T

L

L

K

Q

D

D

I

V

S

S

V

F

Q

T

F

V

Q

M

P

P

G

G

S

Q

F

T

T

L

A

A

L

L

L

V

G

G

P

P

N

S

G

G

A

A

G

G

K

K

S

S

T

T

L

I

L

L

K

R

A

L

L

L

C

F

Q

R

E

F

I

Y

P

D

A

I

A

S

R

S

D

G

S

C

I

I

R

R

L

I

G

D

H

G

C

Q

P

D

L

I

V

S

D

Q

W

V

P

T

R

Q

A

A

E

S

L

L

A

R

K

S

S

H

L

I

A

G

V

V

L

V

P

P

Q

Q

H

D

A

T

S

V

L

L

T

-

F

F

P

N

F

D

K

T

V

I

H

A

E

D

V

N

V

I

A

R

M

Y

G

G

L

-

Y

-

P

-

L

-

T

-

L

-

S

-

Q

-

K

-

E

-

G

-

Q

-

L

R

V

V

T

T

K

A

W

G

L

N

T

D

E

E

V

V

E

E

I

A

-

A

-

Q

-

A

-

G

V

I

H

H

L

D

A

A

Q

I

R

M

S

A

Y

F

P

P

-

E

-

G

-

Y

-

R

-

T

-

Q

-

V

-

G

-

E

-

R

-

G

-

L

T

K

L

L

S

S

G

G

E

E

K

K

Q

Q

R

R

V

V

Q

A

L

I

A

A

R

R

V

T

L

I

T

L

Q

K

L

A

S

-

Q

-

S

-

P

-

F

-

P

P

P

G

I

I

L

I

L

L

D

D

E

E

P

A

T

T

S

S

A

A

L

L

D

D

L

T

A

S

Q

N

Q

E

H

R

K

A

V

I

L

Q

A

A

-

S

L

L

A

A

K

K

N

V

L

C

A

A

H

N

K

R

H

-

A

-

Y

-

T

T

V

T

I

I

V

V

L

A

H

H

D

R

L

L

N

S

Q

T

A

V

R

N

Y

-

S

A

D

D

R

Q

V

I

I

L

V

V

L

I

K

K

H

D

G

G

E

C

I

I

V

V

S

E

E

R

G

G

S

R

A

H

S

E

K

A

A

L

L

L

S

S

Sites not aligning to the query:

binding glutathione: 111, 135, 139, 204, 310, 314, 317
binding cholesterol hemisuccinate: 177, 181, 184, 280

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
31% identity, 78% coverage: 35:246/272 of query aligns to 21:230/343 of P30750

query
sites
P30750

L

L

K

N

D

N

I

V

S

S

V

L

Q

H

F

V

Q

P

P

A

G

G

S

Q

F

I

T

Y

A

G

L

V

L

I

G

G

P

A

N
x
S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

K

R

A

C

L

V

C

N

Q

L

E

L

I

E

P

R

A

P

A

T

R

E

D

G

S

S

I

V

R

L

L

V

G

D

H

G

C

Q

P

E

L

L

V

T

D

T

W

L

P

S

R

E

A

S

E

E

L

L

A

T

K

K

S

A

-

R

-

Q

L

I

A

G

V

M

L

I

P

F

Q

Q

H

H

A

F

S

N

L

L

T

L

F

S

P

S

F

R

K

T

V

V

H

F

E

G

V

N

V

V

A

A

M

L

G

P

L

L

Y

E

P

L

L

D

T

N

L

T

S

P

Q

K

K

D

E

E

G

V

Q

K

Q

R

L

R

V

V

T

T

K

E

W

L

L

L

T

S

E

-

V

-

E

-

I

L

V

V

H

G

L

L

A

G

Q

D

R

K

-

H

-

D

S

S

Y

Y

P

P

T

S

-

N

L

L

S

S

G

G

E

Q

K

K

Q

Q

R

R

V

V

Q

A

L

I

A

A

R

R

V

A

L

L

T

-

Q

-

L

-

S

-

Q

-

S

A

P

S

F

N

P

P

P

K

I

V

L

L

L

C

D

D

E
|
E

P

A

T

T

S

S

A

A

L

L

D

D

L

P

A

A

Q

T

H

R

K

S

V

I

L

L

A

E

L

L

A

L

K

K

N

D

L

I

A

N

H

R

K

R

H

L

A

G

Y

L

T

T

V

I

I

L

V

L

V

I

L

T

H

H

D

E

L

M

N

D

Q

V

A

V

A

K

R

R

Y

I

S

C

D

D

R

C

V

V

I

A

V

V

L

I

K

S

H

N

G

G

E

E

I

L

V

I

S

E

E

Q

G

D

S

T

A

V

S

S

K

E

A

V

L

F

S

S

40:46 (vs. 54:60, 86% identical) binding
E166 (= E182) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
29% identity, 82% coverage: 18:239/272 of query aligns to 1:215/371 of 3puyA

query
sites
3puyA

A

A

Q

S

L

V

S

Q

V

L

D

Q

R

N

L

V

S

T

Y

K

A

A

I
x
W

G

G

D

E

K

V

A

V

V

V

L

S

K

K

D

D

I

I

S

N

V

L

Q

D

F

I

Q

H

P

E

G

G

S

E

F

F

T

V

A

V

L

F

L

V

G

G

P

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

K

R

A

M

L

I

C

A

Q

G

E

L

I

E

P

T

A

I

A

T

R

S

D

G

S

D

I

L

R

F

L

I

G

G

H

E

C

K

P

R

L

M

V

N

D

D

W

T

P

P

R

P

A

A

E

E

L

-

A

-

K

R

S

G

L

V

A

G

V

M

L

V

P

F

Q
|
Q

H

S

A

Y

S

A

L

L

T

Y

F

P

P

H

F

L

K

S

V

V

H

A

E

E

V

N

V

M

A

S

M

F

G

G

L

L

Y

K

P

L

L

A

T

G

L

A

S

K

Q

K

K

E

E

V

G

I

Q

N

Q

Q

L

R

V

V

T

N

K

Q

W

V

L

A

T

E

E

V

V

L

E

Q

I

L

V

A

H

H

L

L

A

L

Q

D

R
|
R

S

K

Y

P

P

K

T
x
A

L

L

S
|
S

G

G

G
|
G

E
x
Q

K

R

Q

Q

R

R

V

V

Q

A

L

I

A

G

R

R

V

T

L

L

T

V

Q

-

L

-

S

-

Q

-

S

-

P

A

F

E

P

P

P

S

I

V

L

F

L

L

D

D

E
|
E

P

P

T

L

S

S

A

N

L

L

D

D

L

A

A

A

Q

L

Q

R

H

V

K

Q

V

M

L

R

A

I

L

E

A

I

K

S

N

R

L

L

A

H

H

K

K

R

H

L

A

G

Y

R

T

T

V

M

I

I

V

Y

V

V

L

T

H
|
H

D

D

L

Q

N

V

Q

E

A

A

A

M

R

T

Y

L

S

A

D

D

R

K

V

I

I

V

V

V

L

L

K

D

H

A

G

G

E

R

I

V

V

A

S

Q

E

V

G

G

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ I29), S37 (≠ N54), G38 (= G55), C39 (≠ A56), G40 (= G57), K41 (= K58), S42 (= S59), T43 (= T60), Q81 (= Q100), R128 (= R147), A132 (≠ T151), S134 (= S153), G136 (= G155), Q137 (≠ E156), E158 (= E182), H191 (= H215)
binding magnesium ion: S42 (= S59), Q81 (= Q100)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
29% identity, 82% coverage: 18:239/272 of query aligns to 1:215/371 of 3puxA

query
sites
3puxA

A

A

Q

S

L

V

S

Q

V

L

D

Q

R

N

L

V

S

T

Y

K

A

A

I
x
W

G

G

D

E

K

V

A

V

V

V

L

S

K

K

D

D

I

I

S

N

V

L

Q

D

F

I

Q

H

P

E

G

G

S

E

F

F

T

V

A

V

L

F

L

V

G

G

P

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

K

R

A

M

L

I

C

A

Q

G

E

L

I

E

P

T

A

I

A

T

R

S

D

G

S

D

I

L

R

F

L

I

G

G

H

E

C

K

P

R

L

M

V

N

D

D

W

T

P

P

R

P

A

A

E

E

L

-

A

-

K

R

S

G

L

V

A

G

V

M

L

V

P

F

Q
|
Q

H

S

A

Y

S

A

L

L

T

Y

F

P

P

H

F

L

K

S

V

V

H

A

E

E

V

N

V

M

A

S

M

F

G

G

L

L

Y

K

P

L

L

A

T

G

L

A

S

K

Q

K

K

E

E

V

G

I

Q

N

Q

Q

L

R

V

V

T

N

K

Q

W

V

L

A

T

E

E

V

V

L

E

Q

I

L

V

A

H

H

L

L

A

L

Q

D

R
|
R

S

K

Y

P

P

K

T

A

L

L

S
|
S

G

G

G
|
G

E
x
Q

K

R

Q

Q

R

R

V

V

Q

A

L

I

A

G

R

R

V

T

L

L

T

V

Q

-

L

-

S

-

Q

-

S

-

P

A

F

E

P

P

P

S

I

V

L

F

L

L

D

D

E

E

P

P

T

L

S

S

A

N

L

L

D

D

L

A

A

A

Q

L

Q

R

H

V

K

Q

V

M

L

R

A

I

L

E

A

I

K

S

N

R

L

L

A

H

H

K

K

R

H

L

A

G

Y

R

T

T

V

M

I

I

V

Y

V

V

L

T

H
|
H

D

D

L

Q

N

V

Q

E

A

A

A

M

R

T

Y

L

S

A

D

D

R

K

V

I

I

V

V

V

L

L

K

D

H

A

G

G

E

R

I

V

V

A

S

Q

E

V

G

G

binding adenosine-5'-diphosphate: W12 (≠ I29), G38 (= G55), C39 (≠ A56), G40 (= G57), K41 (= K58), S42 (= S59), T43 (= T60), R128 (= R147), S134 (= S153), Q137 (≠ E156)
binding beryllium trifluoride ion: S37 (≠ N54), G38 (= G55), K41 (= K58), Q81 (= Q100), S134 (= S153), G136 (= G155), H191 (= H215)
binding magnesium ion: S42 (= S59), Q81 (= Q100)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
29% identity, 82% coverage: 18:239/272 of query aligns to 1:215/371 of 3puwA

query
sites
3puwA

A

A

Q

S

L

V

S

Q

V

L

D

Q

R

N

L

V

S

T

Y

K

A

A

I
x
W

G

G

D

E

K

V

A

V

V
|
V

L

S

K

K

D

D

I

I

S

N

V

L

Q

D

F

I

Q

H

P

E

G

G

S

E

F

F

T

V

A

V

L

F

L

V

G

G

P

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

K

R

A

M

L

I

C

A

Q

G

E

L

I

E

P

T

A

I

A

T

R

S

D

G

S

D

I

L

R

F

L

I

G

G

H

E

C

K

P

R

L

M

V

N

D

D

W

T

P

P

R

P

A

A

E

E

L

-

A

-

K

R

S

G

L

V

A

G

V

M

L

V

P

F

Q
|
Q

H

S

A

Y

S

A

L

L

T

Y

F

P

P

H

F

L

K

S

V

V

H

A

E

E

V

N

V

M

A

S

M

F

G

G

L

L

Y

K

P

L

L

A

T

G

L

A

S

K

Q

K

K

E

E

V

G

I

Q

N

Q

Q

L

R

V

V

T

N

K

Q

W

V

L

A

T

E

E

V

V

L

E

Q

I

L

V

A

H

H

L

L

A

L

Q

D

R
|
R

S

K

Y

P

P

K

T
x
A

L

L

S
|
S

G
|
G

E
x
Q

K

R

Q

Q

R

R

V

V

Q

A

L

I

A

G

R

R

V

T

L

L

T

V

Q

-

L

-

S

-

Q

-

S

-

P

A

F

E

P

P

P

S

I

V

L

F

L

L

D

D

E
|
E

P

P

T

L

S

S

A

N

L

L

D

D

L

A

A

A

Q

L

Q

R

H

V

K

Q

V

M

L

R

A

I

L

E

A

I

K

S

N

R

L

L

A

H

H

K

K

R

H

L

A

G

Y

R

T

T

V

M

I

I

V

Y

V

V

L

T

H
|
H

D

D

L

Q

N

V

Q

E

A

A

A

M

R

T

Y

L

S

A

D

D

R

K

V

I

I

V

V

V

L

L

K

D

H

A

G

G

E

R

I

V

V

A

S

Q

E

V

G

G

binding adenosine-5'-diphosphate: W12 (≠ I29), V17 (= V34), G38 (= G55), C39 (≠ A56), G40 (= G57), K41 (= K58), S42 (= S59), T43 (= T60), R128 (= R147), A132 (≠ T151), S134 (= S153), Q137 (≠ E156)
binding tetrafluoroaluminate ion: S37 (≠ N54), G38 (= G55),