SitesBLAST
Comparing 7026177 FitnessBrowser__ANA3:7026177 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4cukA Structure of salmonella d-lactate dehydrogenase in complex with nadh
64% identity, 100% coverage: 1:329/329 of query aligns to 1:329/330 of 4cukA
- active site: S101 (= S101), R234 (= R234), D258 (= D258), E263 (= E263), H295 (= H295)
- binding 1,4-dihydronicotinamide adenine dinucleotide: Y100 (= Y100), G153 (= G153), K154 (= K154), I155 (= I155), F173 (= F173), D174 (= D174), P175 (= P175), H204 (= H204), C205 (= C205), P206 (= P206), N211 (= N211), T232 (= T232), Y260 (= Y260), H295 (= H295), A297 (= A297)
5z20F The ternary structure of d-lactate dehydrogenase from pseudomonas aeruginosa with nadh and oxamate (see paper)
57% identity, 98% coverage: 1:321/329 of query aligns to 8:328/336 of 5z20F
- active site: S108 (= S101), R241 (= R234), D265 (= D258), E270 (= E263), H302 (= H295)
- binding 1,4-dihydronicotinamide adenine dinucleotide: Y107 (= Y100), G160 (= G153), Q161 (≠ K154), I162 (= I155), Y180 (≠ F173), D181 (= D174), P182 (= P175), C212 (= C205), P213 (= P206), T218 (≠ N211), T239 (= T232), G240 (≠ S233), R241 (= R234), H302 (= H295), A304 (= A297)
5z21B The ternary structure of d-lactate dehydrogenase from fusobacterium nucleatum with nadh and oxamate (see paper)
50% identity, 99% coverage: 2:328/329 of query aligns to 3:329/331 of 5z21B
- active site: S101 (= S101), R235 (= R234), D259 (= D258), E264 (= E263), H296 (= H295)
- binding 1,4-dihydronicotinamide adenine dinucleotide: Y100 (= Y100), I105 (≠ V105), G153 (= G153), K154 (= K154), I155 (= I155), D174 (= D174), L175 (≠ P175), P207 (= P206), T212 (≠ N211), T233 (= T232), G234 (≠ S233), R235 (= R234), H296 (= H295), Y299 (≠ F298)
8grvA Dictyostelium discoideum lactate dehydrogenase (dicldha)with NAD
49% identity, 95% coverage: 1:314/329 of query aligns to 3:317/336 of 8grvA
- binding nicotinamide-adenine-dinucleotide: V106 (= V105), G154 (= G153), N155 (≠ K154), I156 (= I155), D176 (= D174), I177 (≠ P175), I178 (≠ Y176), T208 (≠ C205), P209 (= P206), T214 (≠ N211), V235 (≠ T232), H298 (= H295), A300 (= A297), W301 (≠ F298)
4zgsA Identification of the pyruvate reductase of chlamydomonas reinhardtii (see paper)
52% identity, 88% coverage: 38:328/329 of query aligns to 48:344/346 of 4zgsA
- active site: S111 (= S101), R244 (= R234), D268 (= D258), E273 (= E263), H311 (= H295)
- binding nicotinamide-adenine-dinucleotide: Y110 (= Y100), G163 (= G153), A164 (≠ K154), I165 (= I155), D184 (= D174), C215 (= C205), P216 (= P206), L218 (≠ T208), S220 (≠ D210), T221 (≠ N211), S243 (= S233), H311 (= H295), F314 (= F298)
3kb6B Crystal structure of d-lactate dehydrogenase from aquifex aeolicus complexed with NAD and lactic acid (see paper)
38% identity, 84% coverage: 46:322/329 of query aligns to 44:321/334 of 3kb6B
- active site: S97 (= S101), R231 (= R234), D255 (= D258), E260 (= E263), H294 (= H295)
- binding lactic acid: F49 (= F51), S72 (≠ C76), V73 (≠ A77), G74 (= G78), Y96 (= Y100), R231 (= R234), H294 (= H295)
- binding nicotinamide-adenine-dinucleotide: V73 (≠ A77), Y96 (= Y100), V101 (= V105), G150 (= G153), R151 (≠ K154), I152 (= I155), D171 (= D174), V172 (≠ P175), P203 (= P206), T229 (= T232), A230 (≠ S233), R231 (= R234), H294 (= H295), A296 (= A297), Y297 (≠ F298)
1dxyA Structure of d-2-hydroxyisocaproate dehydrogenase (see paper)
33% identity, 98% coverage: 1:324/329 of query aligns to 1:325/330 of 1dxyA
- active site: S101 (= S101), R234 (= R234), D258 (= D258), E263 (= E263), H295 (= H295)
- binding 2-oxo-4-methylpentanoic acid: V77 (≠ A77), Y100 (= Y100), Y298 (≠ F298)
- binding nicotinamide-adenine-dinucleotide: Y100 (= Y100), G152 (= G151), G154 (= G153), H155 (≠ K154), I156 (= I155), Y174 (≠ F173), D175 (= D174), P176 (= P175), H204 (= H204), V205 (≠ C205), P206 (= P206), N211 (= N211), T232 (= T232), A233 (≠ S233), R234 (= R234), H295 (= H295), Y298 (≠ F298)
P17584 D-2-hydroxyisocaproate dehydrogenase; D-HICDH; EC 1.1.1.- from Lacticaseibacillus paracasei (Lactobacillus paracasei) (see paper)
33% identity, 98% coverage: 1:324/329 of query aligns to 1:325/333 of P17584
P26297 D-lactate dehydrogenase; D-LDH; D-specific 2-hydroxyacid dehydrogenase; EC 1.1.1.28 from Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC 11842 / DSM 20081 / BCRC 10696 / JCM 1002 / NBRC 13953 / NCIMB 11778 / NCTC 12712 / WDCM 00102 / Lb 14) (see 2 papers)
35% identity, 91% coverage: 26:324/329 of query aligns to 28:326/333 of P26297
- HI 156:157 (≠ KI 154:155) binding
- D176 (= D174) binding
- H206 (= H204) mutation to Q: Increase of activity.
- VP 207:208 (≠ CP 205:206) binding
- N213 (= N211) binding
- R236 (= R234) mutation to K: Decrease of activity.
- D260 (= D258) binding ; mutation to N: Decrease of activity.
- E265 (= E263) mutation to Q: Decrease of activity.
- H297 (= H295) mutation to Q: 90% loss of activity.
1j49A Insights into domain closure, substrate specificity and catalysis of d-lactate dehydrogenase from lactobacillus bulgaricus (see paper)
34% identity, 91% coverage: 26:324/329 of query aligns to 28:326/332 of 1j49A
- active site: S103 (= S101), R236 (= R234), D260 (= D258), E265 (= E263), H297 (= H295)
- binding nicotinamide-adenine-dinucleotide: Y102 (= Y100), I107 (≠ V105), G153 (= G151), G155 (= G153), I157 (= I155), Y175 (≠ F173), D176 (= D174), I177 (≠ P175), V207 (≠ C205), P208 (= P206), N213 (= N211), V234 (≠ T232), S235 (= S233), R236 (= R234), H297 (= H295), A299 (= A297), F300 (= F298)
4prlA Crystal structure of d-lactate dehydrogenase with NAD+ from lactobacillus jensenii (see paper)
33% identity, 98% coverage: 1:324/329 of query aligns to 1:324/330 of 4prlA
- binding nicotinamide-adenine-dinucleotide: Y101 (= Y100), I106 (≠ V105), V154 (≠ T152), G155 (= G153), H156 (≠ K154), I157 (= I155), Y175 (≠ F173), D176 (= D174), H205 (= H204), T206 (≠ C205), P207 (= P206), A233 (≠ T232), A234 (≠ S233), D259 (= D258), H295 (= H295), A297 (= A297)
2dldA D-lactate dehydrogenase complexed with nadh and oxamate
32% identity, 92% coverage: 26:328/329 of query aligns to 28:330/337 of 2dldA
- active site: S103 (= S101), R236 (= R234), D260 (= D258), E265 (= E263), H297 (= H295)
- binding 1,4-dihydronicotinamide adenine dinucleotide: T154 (= T152), G155 (= G153), H156 (≠ K154), I157 (= I155), D176 (= D174), I177 (≠ P175), V207 (≠ C205), P208 (= P206), N213 (= N211), C234 (≠ T232), S235 (= S233), H297 (= H295)
Sites not aligning to the query:
P30901 D-lactate dehydrogenase; D-LDH; D-specific 2-hydroxyacid dehydrogenase; EC 1.1.1.28 from Lactobacillus helveticus (Lactobacillus suntoryeus) (see paper)
32% identity, 92% coverage: 26:328/329 of query aligns to 28:330/337 of P30901
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
2yq5C Crystal structure of d-isomer specific 2-hydroxyacid dehydrogenase from lactobacillus delbrueckii ssp. Bulgaricus: NAD complexed form (see paper)
31% identity, 98% coverage: 2:323/329 of query aligns to 2:325/331 of 2yq5C
- active site: S102 (= S101), R236 (= R234), D260 (= D258), E265 (= E263), H297 (= H295)
- binding nicotinamide-adenine-dinucleotide: Y101 (= Y100), I106 (≠ V105), V155 (≠ T152), G156 (= G153), H157 (≠ K154), I158 (= I155), Y176 (≠ F173), D177 (= D174), V178 (≠ P175), H206 (= H204), T207 (≠ C205), P208 (= P206), A235 (≠ S233), R236 (= R234), H297 (= H295), F300 (= F298)
3ddnB Crystal structure of hydroxypyruvic acid phosphate bound d-3- phosphoglycerate dehydrogenase in mycobacterium tuberculosis (see paper)
35% identity, 92% coverage: 19:322/329 of query aligns to 15:305/525 of 3ddnB
3dc2A Crystal structure of serine bound d-3-phosphoglycerate dehydrogenase from mycobacterium tuberculosis (see paper)
35% identity, 92% coverage: 19:322/329 of query aligns to 14:304/526 of 3dc2A
Sites not aligning to the query:
1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
33% identity, 85% coverage: 24:303/329 of query aligns to 21:286/304 of 1wwkA
- active site: S96 (= S101), R230 (= R234), D254 (= D258), E259 (= E263), H278 (= H295)
- binding nicotinamide-adenine-dinucleotide: V100 (= V105), G146 (= G151), F147 (≠ T152), G148 (= G153), R149 (≠ K154), I150 (= I155), Y168 (≠ F173), D169 (= D174), P170 (= P175), V201 (≠ C205), P202 (= P206), T207 (≠ N211), T228 (= T232), S229 (= S233), D254 (= D258), H278 (= H295), G280 (≠ A297)
6rj5A Crystal structure of phgdh in complex with compound 39 (see paper)
35% identity, 59% coverage: 70:263/329 of query aligns to 66:260/301 of 6rj5A
6rj3A Crystal structure of phgdh in complex with compound 15 (see paper)
35% identity, 59% coverage: 70:263/329 of query aligns to 65:259/297 of 6rj3A
7ewhA Crystal structure of human phgdh in complex with homoharringtonine (see paper)
35% identity, 59% coverage: 70:263/329 of query aligns to 66:260/302 of 7ewhA
- binding (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine: L146 (≠ I150), G147 (= G151), L148 (≠ T152), G149 (= G153), R150 (≠ K154), I151 (= I155), G152 (= G156), D170 (= D174), H201 (= H204), T202 (≠ C205), P203 (= P206)
Query Sequence
>7026177 FitnessBrowser__ANA3:7026177
MRIGFFSAKHYDMQHFNRTNAAFGAQIEYFDYRLCMQTVKLAEGFEVVCAFVNDSLCEEV
LVELAKGGTKIIAMRCAGFNNVDLVAAKRLGMQVVNVPAYSPESVAEHTVALMLTLNRKI
HKAYQRTRDANFSLEGLVGFNMFGKTVGVIGTGKIGVATIKVLLGFGCKVIAFDPYPNPA
VEALNVEYQDLDTIYANSDIISLHCPLTADNHHLLNKESFAKMKPGVMVINTSRGGLLNA
FDAMEALKLGQIGSLGLDVYENEKELFFEDKSNQIIQDDVFRRLSACHNVIFTGHQAFLT
EEALGAIAHTTLSNVKALLAGERSGNELF
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory