SitesBLAST

P76129 Oxygen sensor protein DosP; Direct oxygen-sensing phosphodiesterase; Direct oxygen sensor protein; Ec DOS; Heme-regulated cyclic di-GMP phosphodiesterase; EC 3.1.4.52 from Escherichia coli (strain K12) (see 6 papers)
31% identity, 41% coverage: 348:599/610 of query aligns to 528:786/799 of P76129

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P76129

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H582 (≠ K401) mutation to A: Loss of cAMP PDE activity.
H586 (= H404) mutation to A: Loss of cAMP PDE activity.

Sites not aligning to the query:

69 mutation H->A,G: Loss of heme binding.
75 mutation H->A,G: No loss of heme binding.
87 mutation M->A,I: Ferrous heme iron changes from an exclusively hexacoordinate low-spin form to an exclusively pentacoordinate high-spin form. Ferric heme iron remains hexacoordinate but becomes a mixture of high and low spin. Increases c-di-GMP PDE activity 7-fold in absence of O(2), CO or NO, no additional increase upon addition of gases (M-A only).; M→H: No change in heme coordination; increases c-di-GMP PDE activity 2-fold in absence of O(2), CO or NO, and 2-fold more upon addition of gases.
89 mutation R->A,E,I: The Fe(2+)-O(2) form loses c-di-GMP PDE activity, due to reduced O(2) affinity and/or increased auto-oxidation. NO and CO forms are less affected.
91 L→F: Alters O(2) binding, increases auto-oxidation.; L→T: Increases auto-oxidation.
107 L→F: Significantly reduces heme-binding affinity; increases auto-oxidation.; L→T: Increases auto-oxidation.

3u2eA Eal domain of phosphodiesterase pdea in complex with 5'-pgpg and mg++
32% identity, 40% coverage: 360:606/610 of query aligns to 1:256/260 of 3u2eA

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binding magnesium ion: E37 (= E395), N98 (= N452), E130 (= E481), D160 (= D511), D160 (= D511), D161 (= D512), E217 (= E567)
binding : Q23 (= Q381), L39 (= L397), A40 (≠ I398), R41 (= R399), P52 (≠ G409), L56 (≠ I413), M72 (≠ V429), N98 (= N452), S100 (≠ D454), D160 (= D511), D161 (= D512), R184 (= R535), E217 (= E567), E220 (= E570), G238 (= G588), F239 (≠ W589)

4hjfA Eal domain of phosphodiesterase pdea in complex with c-di-gmp and ca++
32% identity, 40% coverage: 360:606/610 of query aligns to 3:258/263 of 4hjfA

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binding 9,9'-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one): Q25 (= Q381), L41 (= L397), A42 (≠ I398), R43 (= R399), P54 (≠ G409), L58 (≠ I413), M74 (≠ V429), N100 (= N452), S102 (≠ D454), R186 (= R535), G220 (= G568), E222 (= E570), G240 (= G588), F241 (≠ W589)
binding calcium ion: E39 (= E395), N100 (= N452), E132 (= E481), D162 (= D511), D162 (= D511), E219 (= E567)

4rnhA Pamora tandem diguanylate cyclase - phosphodiesterase, c-di-gmp complex (see paper)
30% identity, 41% coverage: 348:599/610 of query aligns to 159:417/423 of 4rnhA

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4rnhA

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binding 9,9'-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one): Q193 (= Q381), L209 (= L397), L210 (≠ I398), R211 (= R399), P222 (= P410), N266 (= N452), D328 (= D511), R352 (= R535), G386 (= G568), E388 (= E570), G406 (= G588), Y407 (≠ W589)
binding magnesium ion: E207 (= E395), N266 (= N452), E298 (= E481), D328 (= D511)

3n3tB Crystal structure of putative diguanylate cyclase/phosphodiesterase complex with cyclic di-gmp (see paper)
30% identity, 39% coverage: 362:600/610 of query aligns to 4:247/261 of 3n3tB

query
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3n3tB

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L

S

I

H

V

I

A

I

L

D

S

R

D

F

W

V

V

I

L

N

E

T

A

V

C

A

C

E

T

D

Q

L

L

A

R

R

A

W

W

E

Q

L

Q

E

Q

G

G

F

R

Y

A

P

A

K

D

-

D

-

L

-

T

V

L

S

S

I

V

N
|
N

I

I

D

S

P

T

N

R

N

Q

I

F

T

E

D

G

P

E

Q

H

L

L

L

T

A

R

T

A

M

V

N

D

E

R

-

A

-

L

-

A

R

R

L

S

G

G

S

L

V

R

A

P

N

D

R

C

V

L

E

E

I

L

E
|
E

M

I

L

T

E

E

S

N

A

V

F

M

M

L

L

V

D

M

L

T

N

D

R

E

I

V

D

R

N

T

S

C

M

L

K

D

Q

A

L

L

K

R

Q

A

H

R

G

G

F

V

S

R

F

L

F

A

L

L

D
|
D

F

F

G

G

T

T

G

G

F

Y

S

S

L

L

S

S

L

Y

L

L

S

S

R

Q

I

L

N

P

V

F

D

H

G

G

I

L

K

K

L

I

D

D

R
x
Q

S

S

I

F

L

V

A

R

N

K

-

I

T

P

C

A

E

H

P

P

K

S

G

E

R

T

T

Q

L

I

Y

V

L

T

Q

T

I

I

C

L

K

A

L

L

C

A

N

R

S

G

L

L

G

G

F

M

S

E

L

V

I

V

A

A

E
|
E

G
|
G

V

I

E
|
E

T

T

P

A

E

Q

A

Y

E

A

F

F

V

L

N

R

A

D

A

R

G

G

V

C

S

E

Y

F

V

G

Q

Q

G
|
G

W
x
N

L

L

Y

-

A

-

K

-

A

-

M

M

P

S

G

T

P

P

E

Q

A

A

binding 9,9'-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one): Q24 (= Q381), L40 (= L397), V41 (≠ I398), R42 (= R399), P53 (≠ G409), N99 (= N452), D161 (= D511), Q185 (≠ R535), E218 (= E567), G219 (= G568), E221 (= E570), G239 (= G588), N240 (≠ W589)
binding magnesium ion: E38 (= E395), N99 (= N452), E131 (= E481), D161 (= D511), D161 (= D511), D162 (= D512), E218 (= E567)

5m3cB Structure of the hybrid domain (ggdef-eal) of pa0575 from pseudomonas aeruginosa pao1 at 2.8 ang. With gtp and ca2+ bound to the active site of the ggdef domain (see paper)
29% identity, 41% coverage: 348:596/610 of query aligns to 159:414/426 of 5m3cB

query
sites
5m3cB

Q

E

H

Y

Y

Y

S

T

Q

R

Q

E

Q

L

S

T

E

Y

S

L

L

A

K

T

A

E

E

R

L

M

N

A

L

L

E

E

K

T

T

E

I

L

K

R

E

R

V

A

L

L

A

E

-

R

G

D

E

E

L

L

Q

Q

L

L

H

Y

Y

Y

Q

Q

P

P

K

K

I

L

A

S

L

L

S

E

T

S

N

G

H

L

V

L

V

V

G

G

Y

A

E
|
E

A

A

L

L

I

V

R

R

L

W

K

Y

D

H

E

P

-

L

H

F

G

G

K

E

L

I

K

S

G

P

P

E

Y

R

F

F

I

I

D

P

A

L

F

A

Q

E

R

D

A

C

G

G

Y

L

S

I

H

L

I

P

I

L

D

G

R

D

F

W

V

V

I

L

N

E

T

H

V

A

A

C

E

Q

D

Q

L

M

A

G

R

E

W

W

E

Q

-

K

L

L

E

H

G

A

F

P

Y

F

P

G

K

P

V

L

S

S

I

V

N
|
N

I

L

D

A

P

G

N

A

N

Q

I

L

T

G

D

Q

P

P

Q

Q

L

L

L

I

A

E

T

R

M

L

N

E

-

Q

-

L

E

E

R

Q

L

S

G

G

S

L

V

E

A

P

N

S

R

R

V

L

E

Q

I

L

E
|
E

M

I

L

T

E

E

S

S

A

F

F

I

M

M

L

N

D

Q

L

T

N

E

R

E

I

A

D

L

N

A

S

V

M

L

K

H

Q

G

L

L

K

K

Q

R

H

L

G

G

F

V

S

Q

F

L

F

A

L

I

D
|
D

D

D

F

F

G

G

T

T

G

G

F

Y

S

S

L

L

S

S

L

Y

L

L

S

K

R

R

I

L

N

P

V

L

D

D

G

I

I

L

K

K

L

I

D

D

R

K

S

S

I

F

L

I

A

R

N

G

-

L

T

P

C

D

E

D

P

P

K

H

G

D

R

A

T

A

L

I

Y

T

L

R

Q

A

I

I

C

I

K

A

L

L

C

G

N

R

S

S

L

M

G

Q

F

L

S

T

L

V

I

I

A

A

E

E

G

G

V

V

E

E

T

T

P

E

E

G

E

Q

A

Q

E

S

F

F

V

L

N

T

A

H

A

E

G

G

V

C

S

E

Y

Q

V

I

Q

Q

G

G

W

F

L

V

Y

L

A

S

K

P

A

P

M

L

P

P

binding calcium ion: E207 (= E395), N266 (= N452), E298 (= E481), D328 (= D511)

Sites not aligning to the query:

binding calcium ion: 38, 39, 81
binding guanosine-5'-triphosphate: 41, 42, 43, 46, 51, 55, 58, 80, 81, 151, 155

Q55434 Phytochrome-like protein cph2; Bacteriophytochrome cph2 from Synechocystis sp. (strain PCC 6803 / Kazusa) (see paper)
27% identity, 42% coverage: 344:597/610 of query aligns to 590:850/1276 of Q55434

query
sites
Q55434

R

K

M

N

T

T

E

Y

Q

Q

H

F

Y

Y

S

R

Q

P

Q

Q

Q

D

S

S

E

A

S

P

L

M

K

L

A

D

E

R

L

L

N

T

L

L

E

E

K

S

T

D

I

L

K

R

E

Q

V

A

L

L

A

T

G

N

-

Q

E

E

L

F

Q

V

L

L

H

Y

Y

F

Q

Q

P

P

K

Q

I

V

A

A

L

L

S

D

T

T

N

G

H

K

V

L

G

G

Y

V

E

E

A

A

L

L

I

V

R

R

L

W

K

Q

D

H

E

P

H

R

-

L

G

G

K

Q

L

V

K

A

G

P

P

D

Y

V

F

F

I

I

D

P

A

L

F

A

Q

E

R

E

A

L

G

G

Y

L

S

I

H

N

I

H

I

L

D

G

R

Q

F

W

V

V

I

L

N

E

T

T

V

A

A

C

E

A

D

T

L

H

A

Q

R

H

W

F

E

F

L

R

E

E

-

T

G

G

F

R

Y

R

P

L

K

R

V

M

S

A

I

V

N

N

I

I

D

S

P

A

N

R

N

Q

I

F

T

Q

D

D

P

E

Q

K

L

W

L

L

A

N

T

S

M

V

N

L

E

E

-

C

-

L

-

K

R

R

L

T

G

G

S

M

V

P

A

P

N

E

R

D

V

L

E

E

I

L

E

E

M

I

L

T

E

E

S

S

A

L

F

M

M

M

L

E

D

D

L

I

N

K

R

G

I

T

D

V

N

V

S

L

M

L

K

H

Q

R

L

L

K

R

Q

E

H

E

G

G

F

V

S

Q

F

V

F

A

L

I

D

D

F

F

G

G

T

T

G

G

F

Y

S

S

L

L

S

S

L

I

L

L

S

K

R

Q

I

L

N

P

V

I

D

H

G

R

I

L

K

K

L

I

D

D

R

K

S

S

I

F

L

V

A

N

N

D

T

L

C

L

E

N

P

E

K

G

G

A

R

D

T

T

L

A

Y

I

L

I

Q

Q

-

Y

I

V

C

I

K

D

L

L

C

A

N

N

S

G

L

L

G

N

F

L

S

E

L

T

I

V

A

A

E

E

G

G

V

I

E

E

T

S

P

E

E

A

E

Q

A

L

E

Q

F

R

V

L

N

Q

A

K

A

M

G

G

V

C

S

H

Y

L

V

G

Q

Q

G

G

W

Y

L

F

Y

L

A

T

K

R

A

P

M

L

P

P

G

A

Sites not aligning to the query:

129 C→S: Holoprotein exhibits no photochromic activity.
130 H→F: Chromophore ligating activity (in vitro) is 30-40% lower than wild-type.; H→Q: Chromophore ligating activity (in vitro) is about 10% more efficient than wild-type.

Q9I0R8 Cyclic di-GMP phosphodiesterase PA2567; c-di-GMP PDE; EC 3.1.4.52 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
25% identity, 50% coverage: 305:607/610 of query aligns to 285:587/587 of Q9I0R8

query
sites
Q9I0R8

I

V

G

G

L

L

G

G

V

V

L

L

I

-

Y

-

L

-

P

-

F

-

V

-

R

-

F

-

S

P

L

L

M

A

S

D

E

D

H

T

Y

L

E

D

F

G

D

D

S

Q

E

D

L

W

I

L

K

R

R

L

V

V

Q

V

F

S

Q

A

A

A

D

D

I

D

D

A

R

R

-

D

-

R

-

G

M

V

T

G

E

W

Q

A

H

R

Y

Y

S

N

Q

P

Q

L

S

D

E

Q

S

A

L

Q

K

Q

A

R

E

A

L

F

N

T

L

L

E

L

K

T

T

S

I

L

K

S

E

Q

V

A

L

I

A

G

G

T

E

E

L

E

Q

G

L

F

H

H

-

L

-

V

Y

Y

Q

Q

P

P

K

K

I

I

A

D

L

L

S

P

T

T

N

G

H

R

V

C

V

T

G

G

Y

V

E

E

A

A

L

L

I

L

R

R

L

W

K

R

D

H

E

P

H

Q

-

L

G

G

K

F

L

V

K

S

G

P

P

A

Y

E

F

F

I

V

D

P

A

L

F

A

Q

E

R

K

A

T

G

A

Y

L

S

M

H

R

I

P

I

L

D

S

R

D

F

W

V

V

I

L

N

R

T

H

V

A

A

M

E

A

D

Q

L

L

A

A

R

Q

W

W

E

N

L

A

E

R

G

N

F

I

Y

P

P

L

K

R

V

L

S

A

I

I

N

N

I

V

D

S

P

A

N

S

N

D

I

M

T

E

D

D

P

S

Q

S

L

F

L

L

A

E

T

E

M

A

N

V

E

-

R

R

L

L

G

A

S

K

V

T

A

Y

N

D

-

I

-

D

-

L

-

S

R

A

V

L

E

E

I

L

E

E

M

F

L

T

E
|
E

S

S

A

V

F

L

M

I

L

R

D

D

L

A

N

S

R

A

I

V

D

G

N

S

S

V

M

L

K

L

Q

R

L

A

K

R

Q

E

H

L

G

G

F

M

S

G

F

I

F

A

L

V

D

D

F

F

G

G

T

T

G

G

F

Y

S

S

S

N

L

W

S

T

L

Y

L

L

S

R

R

D

I

L

N

P

V

I

D

T

G

A

I

I

K

K

L

L

D

D

R

Q

S

S

I

F

L

T

A

R

N

D

-

L

T

A

C

G

E

S

P

P

K

K

G

A

R

Q

T

S

L

V

Y

T

L

Q

Q

A

I

V

C

I

K

G

L

L

C

A

N

S

S

Q

L

L

G

G

F

Y

S

R

L

V

I

V

A

A

E

E

G

G

V

I

E

E

T

T

P

H

E

D

E

T

A

F

E

H

F

L

V

L

N

Q

A

A

A

W

G

G

V

C

S

H

Y

E

V

G

Q

Q

G

G

W

Y

L

L

Y

I

A

A

K

Q

A

P

M

M

P

-

G

L

P

P

E

E

A

Q

K

L

A

E

F

D

W

W

L

L

E

R

R

R

E464 (= E484) mutation to A: Does not perturb the oligomeric state and does not affect kcat.

5yrpA Crystal structure of the eal domain of mycobacterium smegmatis dcpa (see paper)
30% identity, 37% coverage: 376:603/610 of query aligns to 2:217/224 of 5yrpA

query
sites
5yrpA

L

L

Q

V

L

L

H

H

Y

Y

Q

L

P

P

K

E

I

I

A

D

L

M

S

R

T

T

N

G

H

E

V

V

V

L

G

A

Y

A

E
|
E

A

A

L

L

I

V

R

R

L

W

K

I

D

N

E

L

H

A

G

G

K

E

L

L

K

-

G

-

P

-

Y

-

F

-

I

-

D

-

A

-

F

-

Q

-

R

-

A

-

G

-

Y

-

S

-

H

-

I

-

D

G

R

R

F

W

V

V

I

L

N

R

T

T

V

A

A

C

E

A

D

E

L

F

A

S

R

R

W

W

E

R

L

A

E

N

G

G

F

V

Y

G

P

R

K

N

V

I

-

V

-

L

S

R

I

I

N
|
N

I

V

D

S

P

P

N

V

N

Q

-

L

I

V

T

T

D

D

P

G

-

F

-

V

Q

E

L

S

L

V

A

A

T

G

M

I

N

M

E

K

R

E

L

F

G

G

S

L

V

P

A

R

N

G

R

S

V

V

E

C

I

L

E
|
E

M

I

L

T

E

E

S

S

A

V

F

V

M

V

L

Q

D

D

L

I

N

E

R

T

I

T

D

R

N

T

S

T

M

L

K

T

Q

G

L

L

K

H

Q

N

H

V

G

G

F

V

S

Q

F

V

F

A

L

I

D
|
D

D

D

F

F

G

G

T

T

G

G

F

Y

S

S

S

V

L

L

S

S

L

L

S

K

R

S

I

L

N

P

V

V

D

D

G

T

I

L

K

K

L

I

D

D

R

R

S

S

I

F

L

V

A

A

N

E

-

L

T

G

C

S

E

N

P

P

K

G

G

D

R

L

T

P

L

I

Y

V

L

R

Q

A

I

V

C

I

K

A

L

L

C

A

N

G

S

A

L

F

G

G

F

L

S

Q

L

L

I

V

A

A

E

E

G

G

V

V

E

E

T

T

P

E

E

R

E

A

A

A

E

L

F

T

V

L

N

L

A

R

A

H

G

G

V

C

S

Y

Y

R

V

A

Q

Q

G

G

W

F

L

L

Y

L

A

S

K

K

A

P

M

I

P

L

G

G

P

S

E

E

A

M

K

Q

A

T

F

L

binding magnesium ion: E21 (= E395), N62 (= N452), E94 (= E481), D124 (= D511)

6hq7A Structure of eal enzyme bd1971 - cgmp bound form (see paper)
29% identity, 36% coverage: 375:593/610 of query aligns to 133:364/377 of 6hq7A

query
sites
6hq7A

E

E

L

F

Q

E

L

L

H

F

Y

Y

Q

Q

P

P

K

I

I

V

A

N

L

L

S

K

T

N

N

K

H

T

V

I

V

T

G

G

Y

C

E
|
E

A

A

L

L

I

L

R

R

L

W

K

N

D

S

-

P

E

Q

H

H

G

G

K

L

L

V

K

S

G

P

P

N

Y

L

F

F

I

I

D

D

A

I

F

I

Q

E

R

N

A

S

G

S

Y

M

S

V

H

I

I

P

I

I

D

G

R

H

F

W

V

I

I

I

N

N

T

Q

V

A

A

L

E

K

D

D

L

L

A

R

R

T

W

I

E

Q

L

D

E

Q

G

L

F

R

Y

L

P

N

K

K

-

K

-

E

-

R

-

M

-

A

-

D

-

F

-

M

V

M

S

S

I

I

N

N

I

I

D

S

P

G

N

R

N

Q

I

F

T

T

D

H

P

S

Q

D

L

F

-

V

-

N

L

L

A

E

T

D

M

L

N

R

E

E

R

K

L

H

G

D

S

L

V

H

A

T

N

Q

R

N

V

I

E

K

I

L

E
|
E

M

M

L

T

E

E

S

R

A

I

F

M

M

M

L

D

D

G

L

A

N

I

R

A

I

I

D

D

N

-

S

A

M

L

K

N

Q

Q

L

C

K

R

Q

S

H

L

G

G

F

Y

S

A

F

I

F

S

L

I

D
|
D

D

D

F

F

G

G

T

T

G

G

F

F

S

S

S

G

L

L

S

Q

L

Y

L

L

S

T

R

Q

I

M

N

P

V

I

D

S

G

F

I

L

K

K

L

I

D

D

R

R

S

S

-

F

I

V

L

M

A

K

N

I

T

L

C

S

E

D

P

P

K

K

G

S

R

K

T

A

L

V

Y

V

L

S

Q

S

I

I

C

I

K

H

L

L

C

A

N

H

S

A

L

M

G

D

F

I

S

E

L

V

I

I

A

A

E

E

G

G

V

I

E

E

T

H

P

H

E

E

A

A

E

L

F

V

V

L

N

E

A

T

A

L

G

G

V

A

S

R

Y

F

V

G

Q

Q

G

G

W

Y

L

L

Y

F

A

S

K

K

binding magnesium ion: E153 (= E395), E252 (= E481), D281 (= D511)

Sites not aligning to the query:

binding cyclic guanosine monophosphate: 12, 31, 42, 51, 52, 53, 54, 55, 62, 63, 64, 103, 104, 106

6hq5A Structure of eal enzyme bd1971 - camp and cyclic-di-gmp bound form (see paper)
29% identity, 36% coverage: 375:593/610 of query aligns to 132:363/376 of 6hq5A

query
sites
6hq5A

E

E

L

F

Q

E

L

L

H

F

Y

Y

Q
|
Q

P

P

K

I

I

V

A

N

L

L

S

K

T

N

N

K

H

T

V

I

V

T

G

G

Y

C

E
|
E

A

A

L
|
L

I

L

R
|
R

L

W

K

N

D

S

-

P

E

Q

H

H

G

G

K

L

L

V

K

S

G

P

P

N

Y

L

F

F

I

I

D

D

A

I

F

I

Q

E

R

N

A

S

G

S

Y

M

S

V

H

I

I

P

I

I

D

G

R

H

F

W

V

I

I

I

N

N

T

Q

V

A

A

L

E

K

D

D

L

L

A

R

R

T

W

I

E

Q

L

D

E

Q

G

L

F

R

Y

L

P

N

K

K

-

K

-

E

-

R

-

M

-

A

-

D

-

F

-

M

V

M

S

S

I

I

N
|
N

I

I

D

S

P

G

N

R

N

Q

I

F

T

T

D

H

P

S

Q

D

L

F

-

V

-

N

L

L

A

E

T

D

M

L

N

R

E

E

R

K

L

H

G

D

S

L

V

H

A

T

N

Q

R

N

V

I

E

K

I

L

E
|
E

M

M

L

T

E

E

S

R

A

I

F

M

M

M

L

D

D

G

L

A

N

I

R

A

I

I

D

D

N

-

S

A

M

L

K

N

Q

Q

L

C

K

R

Q

S

H

L

G

G

F

Y

S

A

F

I

F

S

L

I

D
|
D

F

F

G

G

T

T

G

G

F

F

S

S

S

G

L

L

S

Q

L

Y

L

L

S

T

R

Q

I

M

N

P

V

I

D

S

G

F

I

L

K

K

L

I

D
|
D

R
|
R

S

S

-

F

I

V

L

M

A

K

N

I

T

L

C

S

E

D

P

P

K

K

G

S

R

K

T

A

L

V

Y

V

L

S

Q

S

I

I

C

I

K

H

L

L

C

A

N

H

S

A

L

M

G

D

F

I

S

E

L

V

I

I

A

A

E
|
E

G
|
G

V

I

E
|
E

T

H

P

H

E

E

A

A

E

L

F

V

V

L

N

E

A

T

A

L

G

G

V

A

S

R

Y

F

V

G

Q

Q

G
|
G

W
x
Y

L

L

Y

F

A

S

K

K

binding 9,9'-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one): Q138 (= Q381), L154 (= L397), R156 (= R399), N219 (= N452), D280 (= D511), R304 (= R535), G338 (= G568), E340 (= E570), G358 (= G588), Y359 (≠ W589)
binding calcium ion: E152 (= E395), N219 (= N452), E251 (= E481), D280 (= D511), D280 (= D511), D281 (= D512), D303 (= D534), E337 (= E567)

Sites not aligning to the query:

binding adenosine-3',5'-cyclic-monophosphate: 13, 32, 52, 53, 54, 55, 56, 63, 64, 65

5mf5A Pa3825-eal mg-cdg structure (see paper)
31% identity, 37% coverage: 373:596/610 of query aligns to 13:242/256 of 5mf5A

query
sites
5mf5A

A

A

G

N

E

E

L

F

Q

I

L

P

H

Y

Y

Y

Q
|
Q

P

P

K

L

I

S

A

P

L

G

S

Q

T

G

N

G

H

R

V

W

V

I

G

G

Y

V

E
|
E

A
x
V

L
|
L

I
x
M

R
|
R

L

W

K

R

D

H

-

P

E

R

H

E

G

G

K

L

L

I

K
x
R

G
x
P

P
x
D

Y

L

F

F

I

I

D

P

A

F

F

A

Q

E

R

R

A

S

G

G

Y

L

S

I

H

V

I

P

I

M

D

T

R

R

F

A

V

L

I

M

N

R

T

Q

V

V

A

A

E

E

D

D

L

L

A

G

-

G

-

H

R

A

W

G

E

K

L

L

E

E

-

P

G

G

F

F

Y

H

P

-

K

-

V

I

S

G

I

F

N
|
N

I

I

D

S

P

A

N

T

N

H

I

C

T

H

D

E

P

L

Q

A

L

L

L

V

A

D

T

D

M

C

N

R

E

E

R

L

L

L

G

A

S

A

-

F

-

P

-

G

-

H

-

I

-

T

-

L

V

V

A

L

N
x
E

R

L

V

T

E

E

I

R

E

E

M

L

L

I

E

E

S

S

A

S

F

E

M

V

L

T

D

D

L

-

N

-

R

R

I

L

D

F

N

D

S

E

M

L

K

H

Q

A

L

L

K

-

Q

-

H

-

G

G

F

V

S

K

F

I

F

A

L

I

D
|
D

F

F

G

G

T

T

G

G

F

H

S

S

L

L

S

A

L

Y

L

L

S

R

R

K

I

F

N

Q

V

V

D

D

G

C

I

L

K

K

L

I

D
|
D

R
x
Q

S

S

I

F

L

V

A

A

N

R

-

I

T

G

C

I

E

D

P

T

K

L

G

S

R

G

T

H

L

I

Y

L

L

D

Q

S

I

I

C

V

K

E

L

L

C

S

N

A

S

K

L

L

G

D

F

L

S

D

L

I

I

V

A

A

E

E

G
|
G

V

V

E
|
E

T

T

P

P

E

E

E

Q

A

R

E

D

F

Y

V

L

N

A

A

A

A

R

G

G

V

V

S

D

Y

Y

V

L

Q

Q

G
|
G

W
x
Y

L

L

Y

I

A

G

K

R

A
x
P

M

M

P

P

binding 9,9'-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one): Q21 (= Q381), L37 (= L397), M38 (≠ I398), R39 (= R399), R49 (≠ K408), P50 (≠ G409), D51 (≠ P410), N94 (= N452), Q180 (≠ R535), G214 (= G568), E216 (= E570), G234 (= G588), Y235 (≠ W589), P240 (≠ A594)
binding magnesium ion: E35 (= E395), V36 (≠ A396), N94 (= N452), N94 (= N452), E126 (≠ N476), D156 (= D511), D157 (= D512), D179 (= D534)

5mfuA Pa3825-eal mn-pgpg structure (see paper)
31% identity, 37% coverage: 373:596/610 of query aligns to 13:242/254 of 5mfuA

query
sites
5mfuA

A

A

G

N

E

E

L

F

Q

I

L

P

H

Y

Y

Y

Q
|
Q

P

P

K

L

I

S

A

P

L

G

S

Q

T

G

N

G

H

R

V

W

V

I

G

G

Y

V

E
|
E

A

V

L
|
L

I
x
M

R
|
R

L

W

K

R

D

H

-

P

E

R

H

E

G

G

K

L

L

I

K
x
R

G
x
P

P
x
D

Y

L

F

F

I

I

D

P

A

F

F

A

Q

E

R

R

A

S

G

G

Y

L

S

I

H

V

I

P

I

M

D

T

R

R

F

A

V

L

I

M

N

R

T

Q

V

V

A

A

E

E

D

D

L

L

A

G

-

G

-

H

R

A

W

G

E

K

L

L

E

E

-

P

G

G

F

F

Y

H

P

-

K

-

V

I

S

G

I

F

N
|
N

I

I

D

S

P

A

N

T

N

H

I

C

T

H

D

E

P

L

Q

A

L

L

L

V

A

D

T

D

M

C

N

R

E

E

R

L

L

L

G

A

S

A

-

F

-

P

-

G

-

H

-

I

-

T

-

L

V

V

A

L

N
x
E

R

L

V

T

E

E

I

R

E

E

M

L

L

I

E

E

S

S

A

S

F

E

M

V

L

-

D

-

L

-

N

-

R

-

I

T

D

D

N

R

S

L

M

F

K

D

Q

E

L

L

K

H

Q

A

H

L

G

G

F

V

S

K

F

I

F

A

L

I

D
|
D

F

F

G

G

T

T

G

G

F

H

S

S

L

L

S

A

L

Y

L

L

S

R

R

K

I

F

N

Q

V

V

D

D

G

C

I

L

K

K

L

I

D

D

R
x
Q

S

S

I

F

L

V

A

A

N

R

-

I

T

G

C

I

E

D

P

T

K

L

G

S

R

G

T

H

L

I

Y

L

L

D

Q

S

I

I

C

V

K

E

L

L

C

S

N

A

S

K

L

L

G

D

F

L

S

D

L

I

I

V

A

A

E
|
E

G
|
G

V

V

E
|
E

T

T

P

P

E

E

E

Q

A

R

E

D

F

Y

V

L

N

A

A

A

A

R

G

G

V

V

S

D

Y

Y

V

L

Q

Q

G
|
G

W
x
Y

L

L

Y

I

A

G

K

R

A

P

M

M

P

P

binding guanosine-5'-monophosphate: Q21 (= Q381), L37 (= L397), M38 (≠ I398), R39 (= R399), R49 (≠ K408), P50 (≠ G409), D51 (≠ P410), N94 (= N452), D156 (= D511), D157 (= D512), Q180 (≠ R535), E213 (= E567), G214 (= G568), E216 (= E570), G234 (= G588), Y235 (≠ W589)
binding manganese (ii) ion: E35 (= E395), N94 (= N452), E126 (≠ N476), D156 (= D511)

5m1tA Pamucr phosphodiesterase, c-di-gmp complex (see paper)
27% identity, 37% coverage: 375:599/610 of query aligns to 15:245/247 of 5m1tA

query
sites
5m1tA

E

Q

L

L

Q

V

L

L

H

H

Y

Y

Q
|
Q

P

P

K

K

I

V

A

L

L

A

S

P

T

N

N

G

H

P

V

M

V

I

G

G

Y

V

E
|
E

A

A

L
|
L

I

L

R
|
R

L

W

K

E

D

H

-

P

E

Q

H

H

G

G

K

L

L

I

K

T

G
x
P

P

G

Y

Q

F

F

I
x
L

D

P

A

L

F

A

Q

E

R

K

A

T

G

G

Y

L

S

I

H

V

I

Q

I

I

D

G

R

E

F

W

V
|
V

I

L

N

D

T

E

V

A

A

C

E

R

D

Q

L

M

A

R

R

L

W

W

E

-

L

L

E

D

G

G

F

G

Y

H

P

A

-

D

-

W

K

N

V

I

S

A

I

V

N
|
N

I

L

D

S

P

A

N

L

N

Q

I

F

T

A

D

H

P

A

Q

G

L

L

L

V

A

D

T

S

M

V

N

R

E

N

-

A

-

L

R

R

L

H

G

S

S

L

V

E

A

P

N

S

R

H

V

L

E

I

I

L

E
|
E

M

V

L

T

E

E

S

S

A

T

F

A

M

M

L

R

D

D

L

A

N

D

R

A

I

S

D

L

N

V

S

I

M

L

K

E

Q

Q

L

L

K

S

Q

A

H

M

G

G

F

V

S

G

F

I

F

S

L

I

D
|
D

F

F

G

G

T

T

G

G

F

Y

S

S

L

L

S

L

L

Y

L

L

S

K

R

R

I

L

N

P

V

A

D

S

G

E

I

L

K

K

L

I

D

D

R
|
R

S

G

I

F

L

I

A

N

N

E

T

L

C

A

-

H

E

D

P

S

K

D

G

D

R

A

T

A

L

I

Y

V

L

S

Q

A

I

I

C

V

K

A

L

L

C

G

N

R

S

T

L

L

G

N

F

L

S

K

L

I

I

V

A

A

E
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E

G
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G

V

V

E
|
E

T

T

P

E

E

A

E

Q

A

Q

E

E

F

F

V

L

N

T

A

R

A

L

G

G

V

C

S

N

Y

S

V

L

Q

Q

G
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G

W
x
F

L

L

Y

L

A

G

K

R

A

P

M

M

P

P

G

A

P

E

E

Q

binding 9,9'-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one): Q21 (= Q381), E35 (= E395), L37 (= L397), R39 (= R399), P50 (≠ G409), L54 (≠ I413), V70 (= V429), N94 (= N452), R180 (= R535), G214 (= G568), E216 (= E570), G234 (= G588), F235 (≠ W589)
binding magnesium ion: E35 (= E395), N94 (= N452), E126 (= E481), D156 (= D511), D156 (= D511), D157 (= D512), E213 (= E567)

P24400 PTS system lactose-specific EIICB component; EIICB-Lac; EII-Lac; EC 2.7.1.207 from Lacticaseibacillus casei (Lactobacillus casei) (see paper)
32% identity, 24% coverage: 177:325/610 of query aligns to 266:417/577 of P24400

query
sites
P24400

Q

Q

L

H

V

V

P

S

H

H

L

V

T

L

A

A

S

M

Q

N

F

T

M

M

D

D

L

Y

F

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Y

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G

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G

G

A

A

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T

L

L

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L

V

I

P

I

F

A

I

I

M

F

L

I

F

G

A

A

A

K

R

D

S

S

A

A

Q

T

L

R

K

H

A

I

V

A

G

K

K

I

A

A

A

T

F

P

V

F

P

A

C

I

T

F

F

N

G

I

V

N

N

E

E

I

P

L

V

I

L

Y

F

G

G

L

M

P

P

I

I

F

M

N

N

P

P

R

M

L

L

L

F

V

I

P

P

F

F

I

L

L

A

S

T

P

P

L

I

V

V

N

N

F

V

I

C

L

L

A

F

Y

K

S

F

A

F

I

V

H

S

V

V

G

L

L

G

L

M

S

N

F

S

E

M

G

M

H

Y

S

T

F

M

P

P

W

W

I

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T

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P

P

P

G

L

P

L

I

N

G

A

I

Y

L

I

I

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S

S

T

G

G

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M

A

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P

A

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A

F

F

-

V

F

F

Q

V

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L

L

L

L

T

I

L

G

V

L

L

G

D

V

V

L

A

I

I

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Y

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P

F

F

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I

R

R

R

V

F

Y

-

D

-

S

S

T

L

L

M

L

S

A

E

E

H

E

Sites not aligning to the query:

483 modified: Phosphocysteine; by EIIA; mutation C->H,S: Unable to be phosphorylated.

3pjuA Structure of pseudomonas fluorescence lapd eal domain complexed with c-di-gmp, p6522 (see paper)
28% identity, 36% coverage: 377:596/610 of query aligns to 28:247/249 of 3pjuA

query
sites
3pjuA

Q

E

L

L

H

F

Y

F

Q
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Q

P

P

K

V

I

V

A

A

L

A

-

Q

S

D

T

T

N

Q

H

L

V

V

V

L

G

H

Y

Y

E
x
K

A

V

L
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L

I
x
S

R
|
R

L

L

K

L

D

D

E

E

H

Q

G

G

K

Q

-

T

L

I

K

P

G
x
A

P
x
G

Y

R

F

F

I
x
L

D

P

A

W

F

L

Q

E

R

R

A

F

G

G

Y

W

S

T

H

A

I

R

I

L

D

D

R

R

F

L

V
x
M

I

L

N

E

T

R

V

V

A

L

E

E

D

Q

L

M

A

A

R

-

W

-

E

-

L

-

E

-

G

G

F

H

Y

E

P

E

K

S

V

L

S

A

I

L

N
|
N

I

L

D

S

P

S

N

A

N

T

I

L

T

A

D

D

P

P

Q

Q

L

A

L

L

A

N

T

K

M

V

N

F

E

E

R

I

L

L

G

R

S

A

V

H

A

S

N

N

-

L

-

G

-

A

R

R

V

L

E

T

I

L

E

E

M

I

L

G

E

E

S

E

A

Q

F

-

M

L

L

P

D

E

L

Q

N

A

R

V

I

L

D

E

N

Q

S

L

M

T

K

R

Q

R

L

L

K

R

Q

E

H

L

G

G

F

F

S

S

F

L

F

S

L

L

D
x
Q

D
x
R

F

F

G

G

T

G

G

R

F

F

S

S

S

M

L

I

S

G

L

N

L

L

S

A

R

R

I

L

N

G

V

L

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A

G

Y

I

L

K

K

L

I

D

D

R

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S

I

Y

L

I

A

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N

A

T

I

C

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E

Q

P

E

K

S

G

D

R

K

T

R

L

L

Y

F

L

I

Q

E

-

A

I

I

C

Q

K

R

L

A

C

A

N

H

S

S

L

I

G

D

F

L

S

P

L

L

I

I

A

A

E

E

G
x
R

V

V

E
|
E

T

T

P

E

E

G

E

E

A

L

E

S

F

V

V

I

N

R

A

E

A

M

G

G

V

L

S

Y

Y

G

V

V

Q

Q

G
|
G

W
x
Q

L

L

Y

F

A

G

K

E

A

P

M

K

P

P

binding 9,9'-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one): Q32 (= Q381), K47 (≠ E395), L49 (= L397), S50 (≠ I398), R51 (= R399), A62 (≠ G409), G63 (≠ P410), L66 (≠ I413), M82 (≠ V429), N100 (= N452), Q161 (≠ D511), R162 (≠ D512), R219 (≠ G568), E221 (= E570), G239 (= G588), Q240 (≠ W589)

Q9HX69 Cyclic di-GMP phosphodiesterase RocR; c-di-GMP PDE; Response regulator RocR; EC 3.1.4.52 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 3 papers)
27% identity, 37% coverage: 374:596/610 of query aligns to 154:381/392 of Q9HX69

query
sites
Q9HX69

G

G

E

E

L

F

Q

E

L

A

H

Y

Y

Y

Q
|
Q

P

P

K

K

I

V

A

A

L

L

S

D

T

G

N

G

H

G

V

L

V

I

G

G

Y

A

E
|
E

A

V

L

L

I

A

R
|
R

L

W

K

N

D

H

E

P

H

H

-

L

G

G

K

V

L

L

K

P

G

P

P

S

Y

H

F

F

I

L

D

Y

A

V

F

M

Q

E

R

-

A

-

G

-

Y

T

S

Y

H

N

I

L

I

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D

D

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K

F

L

V

F

I

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N

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A

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E

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D

G

L

L

A

A

-

T

R

R

W

R

E

K

L

L

E

A

G

Q

F

L

Y

G

P

Q

K

P

V

I

S

N

I

L

-

A

-

F

N
|
N

I

V

D

H

P

P

N

S

N

Q

I

L

T

G

D

S

P

R

Q

A

L

L

L

A

A

E

T

N

M

I

N

S

E

A

R

L

L

L

G

T

S

E

V

F

-

H

-

L

-

P

A

P

N

S

R

S

V

V

E

M

I

F

E
|
E

M

I

L
x
T

E
|
E

S

T

A

G

F

L

M

I

L

S

D

A

L

P

N

A

R

S

I

S

D

L

N

E

S

N

M

L

K

V

Q

R

L

L

K
x
R

Q

I

H

M

G

G

F

C

S

G

F

L

F

A

L

M

D
|
D

F
|
F

G

G

T

A

G

G

F

Y

S
|
S

S

S

L

L

S

D

L

R

L

L

S

C

R

E

I

F

N

P

V

F

D

S

G

Q

I

I

K
|
K

L

L

D
|
D

R

R

S

T

I

F

L

V

A

Q

N

K

-

M

T

K

C

T

E

Q

P

P

K

R

G

S

R

C

T

A

L

V

Y

I

L

S

Q

S

I

V

C

V

K

A

L

L

C

A

N

Q

S

A

L

L

G

G

F

I

S

S

L

L

I

V

A

V

E
|
E

G

G

V

V

E
|
E

T

S

P

D

E

E

E

Q

A

R

E

V

F

R

V

L

N

I

A

E

A

L

G

G

V

C

S

S

Y

I

V

A

Q
|
Q

G

G

W

Y

L

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Y

F

A

A

K

R

A

P

M

M

P

P

Q161 (= Q381) mutation to A: 5.1-fold decrease in kcat.
E175 (= E395) binding ; mutation to A: Loss of activity. Activity can be partially restored at elevated Mg(2+) concentrations (up to 500 mM).
R179 (= R399) mutation to A: 29.1-fold decrease in kcat.
N233 (= N452) binding ; mutation to A: Loss of activity. Activity can be fully restored at elevated Mg(2+) concentrations (up to 500 mM).
E265 (= E481) binding ; mutation to A: Loss of activity. Activity can be partially restored at elevated Mg(2+) concentrations (up to 500 mM).
T267 (≠ L483) mutation to A: 13.4-fold decrease in kcat.
E268 (= E484) mutation to A: Loss of activity. Activity can be partially restored at elevated Mg(2+) concentrations (up to 500 mM).; mutation to Q: 446-fold decrease in kcat.
R286 (≠ K502) mutation to W: Thermostable. Shows lower enzymatic activity.
D295 (= D511) binding ; mutation to A: Loss of activity. Activity can be partially restored at elevated Mg(2+) concentrations (up to 500 mM).
D296 (= D512) mutation to A: 33.5-fold decrease in kcat.
F297 (= F513) mutation to A: 33.5-fold decrease in kcat.
S302 (= S518) mutation to A: 4.8-fold decrease in kcat.
K316 (= K532) mutation to A: Loss of activity. Activity can be partially restored at elevated Mg(2+) concentrations (up to 500 mM).
D318 (= D534) mutation to A: 8.4-fold decrease in kcat.
E352 (= E567) mutation E->A,C: Loss of activity.; mutation to D: 30000-fold decrease in kcat.; mutation to Q: 61000-fold decrease in kcat.
E355 (= E570) mutation to A: 1.3-fold decrease in kcat.
Q372 (= Q587) mutation to A: 8.4-fold decrease in kcat.

Sites not aligning to the query:

56 D→N: 5.2-fold decrease in kcat.

6ij2A Crystal structure of a standalone versatile eal protein from vibrio cholerae o395 - 5'-pgpg bound form (see paper)
29% identity, 37% coverage: 375:600/610 of query aligns to 4:225/236 of 6ij2A

query
sites
6ij2A

E

D

L

F

Q

T

L

M

H

A

Y

F

Q
|
Q

P

P

K

I

I

V

A

N

L

C

S

R

T

T

N

K

H

E

V

I

V

F

G

G

Y

Y

E
|
E

A

A

L
|
L

I
x
V

R
|
R

-

G

L

L

K

N

D

N

E

E

H

S

G

A

K

-

L

-

K

-

G

-

P

-

Y

Y

F

S

I

V

D
x
I

A

S

F

R

Q

V

R

N

A

E

G

D

Y

N

S

R

H

Y

I

L

I

F

D

D

R

Q

F

M

V

C

I

R

N

V

T

K

V

A

A

I

E

A

D

L

L

A

A

A

R

K

W

L

E

-

L

-

E

-

G

G

F

L

Y

T

P

S

K

K

V

L

S

S

I

I

N
|
N

I

F

D
x
L

P

P

N

N

N

A

I

I

T

Y

D

V

P

P

Q

E

-

R

-

C

L

I

L

R

A

T

T

T

M

L

N

E

-

A

E

K

R

R

L

Y

G

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S

F

V

P

A

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N

E

R

N

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I

F

E
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E

M

F

L
x
T

E

E

S

A

A

E

F

R

M

V

L

E

D

D

L

V

N

N

R

H

I

I

D

K

N

R

S

I

M

V

K

E

Q

Y

L

Y

K

K

Q

S

H

L

G

G

F

F

S

Q

F

T

F

A

L

I

D
|
D

F

F

G

G

T
x
S

G

G

F
x
Y

S

S

S

G

L

L

S

N

L

L

S

A

R

D

I

F

N

Q

V

T

D

N

G

I

I

V

K

K

L

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D

D

R
x
M

S

G

I

L

L

I

A

R

N

N

T

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C

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A

P

D

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G

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R

T

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S

Y

I

L

M

Q

K

I

N

C

C

-

L

K

K

L

L

C

F

N

S

S

D

L

L

G

N

F

I

S

Q

L

P

I

L

A

A

E
|
E

G
|
G

V

V

E
|
E

T

S

P

H

E

A

E

E

A

F

E

A

F

W

V

L

N

K

A

A

G

G

V

V

S

E

Y

L

V

M

Q

Q

G
|
G

W
x
Y

L

Y

Y

F

A

A

K

K

A

-

M

-

P

P

G

G

P

F

E

E

A

S

binding calcium ion: E24 (= E395), N74 (= N452), E107 (= E481), T109 (≠ L483), D137 (= D511), D137 (= D511), D137 (= D511), D138 (= D512), D138 (= D512), D138 (= D512), S141 (≠ T515), Y143 (≠ F517), E194 (= E567)
binding : Q10 (= Q381), L26 (= L397), V27 (≠ I398), R28 (= R399), I39 (≠ D414), N74 (= N452), L76 (≠ D454), D137 (= D511), M161 (≠ R535), G195 (= G568), E197 (= E570), G215 (= G588), Y216 (≠ W589)

Query Sequence

>7026215 FitnessBrowser__ANA3:7026215
MQLSKIFPLLALISLSFHFAKYLQLSAIVVCSLSLSILLAINAQDTGNVFNLDYVRAILA
DPRIAVLPIISAYLLRFLSAWKGLQLIKAKALSRYLKQHLNLFFPIVIGFIGLFAVVAEA
SIFLEWLFAPFIEGLQQASLGVQLFMRILLTHVLWCFGVHGDNAYLLLIGVDNGLMQLVP
HLTASQFMDLFILYGGSGATLSLIIAIFIGAKDSATRHIAKIATPFAIFNINEILIYGLP
IIFNPRLLVPFILSPLVNFILAYSAIHVGLLSFEGHSFPWITPPLLNAYIASGHMSAVFF
QVLLIGLGVLIYLPFVRRFSLMSEHYEFDSELIKRVQFQADIDRMTEQHYSQQQSESLKA
ELNLEKTIKEVLAGELQLHYQPKIALSTNHVVGYEALIRLKDEHGKLKGPYFIDAFQRAG
YSHIIDRFVINTVAEDLARWELEGFYPKVSINIDPNNITDPQLLATMNERLGSVANRVEI
EMLESAFMLDLNRIDNSMKQLKQHGFSFFLDDFGTGFSSLSLLSRINVDGIKLDRSILAN
TCEPKGRTLYLQICKLCNSLGFSLIAEGVETPEEAEFVNAAGVSYVQGWLYAKAMPGPEA
KAFWLERNSE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory