SitesBLAST

1htpA Refined structures at 2 angstroms and 2.2 angstroms of the two forms of the h-protein, a lipoamide-containing protein of the glycine decarboxylase complex (see paper)
50% identity, 99% coverage: 2:129/129 of query aligns to 1:127/131 of 1htpA

query
sites
1htpA

S

S

N

N

I

V

P

L

T

D

E

G

L

L

K

K

Y

Y

A

A

S

P

S
|
S

H
|
H

E
|
E

W

W

I

V

R

-

K

K

E

H

E

E

D

G

G

S

S

V

Y

A

T

T

V

I

G

G

I

I

T

T

E

D

H

H

A

A

Q

Q

E

D

L
x
H

L

L

G

G

D

E

M

V

V

V

F

F

V

V

E

E

L

L

P

P

E

E

V

P

G

G

D

V

T

S

V

V

T

T

A

K

G

G

D

K

D

G

C

F

A

G

V

A

A

V

E

E

S

S

V

V

K
|
K

A

A

A

T

S

S

D

D

I

V

Y

N

A

S

P

P

I

I

S

S

G

G

E

E

V

V

I

I

A

E

V

V

N

N

E

T

A

G

L

L

E

T

D

G

S

K

P

P

E

G

L

L

V

I

N

N

S

S

D

S

A

P

Y

Y

G

E

E

D

G

G

W

W

F

M

F

I

R

K

V

I

M

K

P

P

S

T

D

S

E

P

S

D

E

E

V

L

D

E

A

S

L

L

D

G

A

A

E

K

G

E

Y

Y

Q

T

A

K

V

F

I

C

D

E

E

E

E
|
E

binding 6-(hydroxyethyldithio)-8-(aminomethylthio)octanoic acid: S12 (= S13), H13 (= H14), E14 (= E15), H34 (≠ L36), K63 (= K65), E127 (= E129)

1hpcB Refined structures at 2 angstroms and 2.2 angstroms of the two forms of the h-protein, a lipoamide-containing protein of the glycine decarboxylase (see paper)
50% identity, 99% coverage: 2:129/129 of query aligns to 1:127/131 of 1hpcB

query
sites
1hpcB

S

S

N

N

I

V

P

L

T

D

E

G

L

L

K

K

Y

Y

A

A

S

P

S

S

H

H

E

E

W

W

I

V

R

-

K

K

E

H

E

E

D

G

G

S

S

V

Y

A

T

T

V

I

G

G

I

I

T

T

E

D

H

H

A

A

Q

Q

E

D

L
x
H

L

L

G

G

D

E

M

V

V

V

F

F

V

V

E

E

L

L

P

P

E

E

V

P

G

G

D

V

T

S

V

V

T

T

A

K

G

G

D

K

D

G

C

F

A

G

V

A

A

V

E

E

S

S

V

V

K
|
K

A

A

A

T

S

S

D

D

I

V

Y

N

A

S

P

P

I

I

S

S

G

G

E

E

V

V

I

I

A

E

V

V

N

N

E

T

A

G

L

L

E

T

D

G

S

K

P

P

E

G

L

L

V

I

N

N

S

S

D

S

A

P

Y

Y

G

E

E

D

G

G

W

W

F

M

F

I

R

K

V

I

M

K

P

P

S

T

D

S

E

P

S

D

E

E

V

L

D

E

A

S

L

L

D

G

A

A

E

K

G

E

Y

Y

Q

T

A

K

V

F

I

C

D

E

E

E

binding lipoic acid: H34 (≠ L36), K63 (= K65)

1hpcA Refined structures at 2 angstroms and 2.2 angstroms of the two forms of the h-protein, a lipoamide-containing protein of the glycine decarboxylase (see paper)
50% identity, 99% coverage: 2:129/129 of query aligns to 1:127/131 of 1hpcA

query
sites
1hpcA

S

S

N

N

I

V

P

L

T

D

E

G

L

L

K

K

Y

Y

A

A

S

P

S

S

H

H

E

E

W

W

I

V

R

-

K

K

E

H

E

E

D

G

G

S

S

V

Y

A

T

T

V

I

G

G

I

I

T

T

E

D

H

H

A

A

Q

Q

E

D

L
x
H

L

L

G

G

D

E

M

V

V

V

F

F

V

V

E

E

L

L

P

P

E

E

V

P

G

G

D

V

T

S

V

V

T

T

A

K

G

G

D

K

D

G

C

F

A

G

V

A

A

V

E

E

S

S

V

V

K
|
K

A

A

A

T

S

S

D

D

I

V

Y

N

A

S

P

P

I

I

S

S

G

G

E

E

V

V

I

I

A

E

V

V

N

N

E

T

A

G

L

L

E

T

D

G

S

K

P

P

E

G

L

L

V

I

N

N

S

S

D

S

A

P

Y

Y

G

E

E

D

G

G

W

W

F

M

F

I

R

K

V

I

M

K

P

P

S

T

D

S

E

P

S

D

E

E

V

L

D

E

A

S

L

L

D

G

A

A

E

K

G

E

Y

Y

Q

T

A

K

V

F

I

C

D

E

E

E

binding 5-[(3S)-1,2-dithiolan-3-yl]pentanoic acid: H34 (≠ L36), K63 (= K65)

1dxmA Reduced form of the h protein from glycine decarboxylase complex (see paper)
50% identity, 99% coverage: 2:129/129 of query aligns to 1:127/131 of 1dxmA

query
sites
1dxmA

S

S

N

N

I

V

P

L

T

D

E

G

L

L

K

K

Y

Y

A

A

S

P

S

S

H

H

E

E

W

W

I

V

R

-

K

K

E

H

E

E

D

G

G

S

S

V

Y

A

T

T

V

I

G

G

I

I

T

T

E

D

H

H

A

A

Q

Q

E

D

L
x
H

L
|
L

G

G

D

E

M

V

V

V

F

F

V

V

E

E

L

L

P

P

E

E

V

P

G

G

D

V

T

S

V

V

T

T

A

K

G

G

D

K

D

G

C

F

A

G

V

A

A

V

E

E

S

S

V

V

K
|
K

A

A

A

T

S

S

D

D

I

V

Y

N

A

S

P

P

I

I

S

S

G

G

E

E

V

V

I

I

A

E

V

V

N

N

E

T

A

G

L

L

E

T

D

G

S

K

P

P

E

G

L

L

V

I

N

N

S

S

D

S

A

P

Y

Y

G

E

E

D

G

G

W

W

F

M

F

I

R

K

V

I

M

K

P

P

S

T

D

S

E

P

S

D

E

E

V

L

D

E

A

S

L

L

D

G

A

A

E

K

G

E

Y

Y

Q

T

A

K

V

F

I

C

D

E

E

E

E
|
E

binding dihydrolipoic acid: H34 (≠ L36), L35 (= L37), K63 (= K65), E127 (= E129)

P23434 Glycine cleavage system H protein, mitochondrial; Lipoic acid-containing protein from Homo sapiens (Human) (see 4 papers)
46% identity, 92% coverage: 9:127/129 of query aligns to 52:169/173 of P23434

query
sites
P23434

K

K

Y

F

A

T

S

E

S

K

H
|
H

E

E

W

W

I

V

R

-

K

T

E

T

E

E

D

N

G

G

S

I

Y

G

T

T

V

V

G

G

I

I

T

S

E
x
N

H

F

A

A

Q
|
Q

E
|
E

L
x
A

L
|
L

G
|
G

D
|
D

M
x
V

V
|
V

F
x
Y

V
x
C

E
x
S

L
|
L

P
|
P

E
|
E

V
|
V

G
|
G

D
x
T

T
x
K

V
x
L

T
x
N

A
x
K

G
x
Q

D
|
D

D
x
E

C
x
F

A
x
G

V
x
A

A
x
L

E
|
E

S
|
S

V
|
V

K
|
K

A
|
A

S
|
S

D
x
E

I
x
L

Y
|
Y

A
x
S

P
|
P

I
x
L

S
|
S

G
|
G

E
|
E

V
|
V

I
x
T

A
x
E

V
x
I

N
|
N

E
|
E

A
|
A

L
|
L

E
x
A

D
x
E

S
x
N

P
|
P

E
x
G

L
|
L

V
|
V

N
|
N

S
x
K

D
x
S

A
x
C

Y
|
Y

G
x
E

E
x
D

G
|
G

W
|
W

F
x
L

F
x
I

R
x
K

V
x
M

M
x
T

P
x
L

S
|
S

D
x
N

E
x
P

S
|
S

E
|
E

V
x
L

D
|
D

A
x
E

L
|
L

L
x
M

D
x
S

A
x
E

E
|
E

G
x
A

Y
|
Y

Q
x
E

A
x
K

V
x
Y

I
|
I

D
x
K

H57 (= H14) to R: in MMDS7; uncertain significance
N73 (≠ E31) to S: in dbSNP:rs8177877
K107 (= K65) modified: N6-lipoyllysine
P115 (= P73) to L: in MMDS7; uncertain significance; reduced lipoylation of DLAT and DLST in homozygous patient cells; no effect on localization to mitochondrion
T148 (≠ M106) to P: in MMDS7; uncertain significance; reduced function in glycine decarboxylation via glycine cleavage system; no effect on localization to mitochondrion

Sites not aligning to the query:

21 S → L: in dbSNP:rs8052579
76:173 natural variant: Missing (in MMDS7; likely pathogenic)

P20821 Glycine cleavage system H protein, mitochondrial; Lipoic acid-containing protein from Bos taurus (Bovine) (see 3 papers)
45% identity, 92% coverage: 9:127/129 of query aligns to 52:169/173 of P20821

query
sites
P20821

K

K

Y

F

A

T

S

E

S

K

H

H

E

E

W

W

I

V

R

-

K

T

E

T

E

E

D

N

G

G

S

V

Y

G

T

T

V

V

G

G

I

I

T

S

E

N

H

F

A

A

Q

Q

E

E

L

A

L

L

G

G

D

D

M

V

V

V

F

Y

V

C

E

S

L

L

P

P

E

E

V

V

G

G

D

T

T

K

V

L

T

N

A

K

G

Q

D

E

C

F

A

G

V

A

A

L

E

E

S

S

V

V

K
|
K

A

A

S

S

D

E

I

L

Y

Y

A

S

P

P

I

L

S

S

G

G

E

E

V

V

I

T

A

E

V

I

N

N

E

K

A

A

L

L

E

A

D

E

S

N

P

P

E

G

L

L

V

V

N

N

S

K

D

S

A

C

Y

Y

G

E

E

D

G

G

W

W

F

L

F

I

R

K

V

M

M

T

P

F

S

S

D

N

E

P

S

S

E

E

V

L

D

D

A

E

L

L

L

M

D

S

A

E

E

E

G

A

Y

Y

Q

E

A

K

V

Y

I

I

D

K

K107 (= K65) modified: N6-lipoyllysine

Sites not aligning to the query:

1:48 modified: transit peptide, Mitochondrion

P11183 Glycine cleavage system H protein, mitochondrial; Lipoic acid-containing protein from Gallus gallus (Chicken) (see paper)
43% identity, 91% coverage: 9:126/129 of query aligns to 43:159/164 of P11183

query
sites
P11183

K

K

Y

F

A

T

S

D

S

K

H

H

E

E

W

W

I

I

R

-

K

S

E

V

E

E

D

N

G

G

S

I

Y

G

T

T

V

V

G

G

I

I

T

S

E

N

H

F

A

A

Q

Q

E

E

L

A

L

L

G

G

D

D

M

V

V

V

F

Y

V

C

E

S

L

L

P

P

E

E

V

I

G

G

D

T

T

K

V

L

T

N

A

K

G

D

D

D

D

E

C

F

A

G

V

A

A

L

E

E

S

S

V

V

K
|
K

A

A

S

S

D

E

I

L

Y

Y

A

S

P

P

I

L

S

T

G

G

E

E

V

V

I

T

A

D

V

I

N

N

E

A

A

A

L

L

E

A

D

D

S

N

P

P

E

G

L

L

V

V

N

N

S

K

D

S

A

C

Y

Y

G

Q

E

D

G

G

W

W

F

L

F

I

R

K

V

M

M

T

P

V

S

E

D

K

E

P

S

A

E

E

V

L

D

D

A

E

L

L

L

M

D

S

A

E

E

D

G

A

Y

Y

Q

E

A

K

V

Y

I

I

K98 (= K65) modified: N6-lipoyllysine

Sites not aligning to the query:

1:39 modified: transit peptide, Mitochondrion

Q9HDV9 Putative glycine cleavage system H protein, mitochondrial; Glycine decarboxylase complex subunit H from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
45% identity, 93% coverage: 10:129/129 of query aligns to 47:165/169 of Q9HDV9

query
sites
Q9HDV9

Y

F

A

T

S

K

S

E

H

H

E

E

W

W

I

V

R

K

K

V

E

D

E

G

D

D

G

V

S

G

Y

-

T

T

V

V

G

G

I

I

T

T

E

S

H

Y

A

A

Q

A

E

N

L

A

L

L

G

G

D

E

M

V

V

V

F

F

V

V

E

E

L

L

P

P

E

E

V

P

G

E

D

T

T

T

V

V

T

S

A

V

G

G

D

D

D

G

C

I

A

G

V

A

A

V

E

E

S

S

V

V

K

K

A

S

A

A

S

S

D

D

I

V

Y

Y

A

S

P

P

I

V

S

S

G

G

E

T

V

V

I

T

A

S

V

I

N

N

E

E

A

S

L

L

E

G

D

D

S

S

P

P

E

D

L

K

V

V

N

S

S

S

D
x
S

A

P

Y

E

G

E

E

E

G

G

W

W

F

I

F

C

R

K

V

I

M

K

P

L

S

S

D

S

E

P

S

D

E

E

V

L

D

K

A

S

L

L

D

N

A

D

E

E

G

S

Y

Y

Q

A

A

Q

V

F

I

C

D

K

E

E

S131 (≠ D95) modified: Phosphoserine

P0DN69 Glycine cleavage system H-like protein; GcvH-L from Streptococcus pyogenes serotype M5 (strain Manfredo) (see paper)
29% identity, 87% coverage: 8:119/129 of query aligns to 1:110/110 of P0DN69

query
sites
P0DN69

L

M

K

K

Y

K

A

I

S

A

S

N

H

Y

E

L

W

L

I

I

R
x
E

K

K

E

T

E

D

D

D

G

-

S

R

Y

Y

T

T

V

I

G

S

I

M

T

T

E

P

H
x
E

A

L

Q

Q

E
x
D

L
x
D

L

I

G

G

D

T

M

I

V

G

F

Y

V

A

E

E

L

F

P

T

E

D

V

-

G

N

D

D

T

H

V

L

T

A

A

V

G
x
D

D
|
D

D

I

C

I

A

L

V

N

A

L

E
|
E

S

A

V

S

K
|
K

A

T

A

V

S

M

D

S

I

V

Y

L

A

S

P

P

I

L

S

A

G

G

E

A

V

V

I

V

A

E

V

R

N

N

E

E

A

A

L

A

E

T

D

L

S

T

P

P

E

T

L

L

V

L

N

N

S

S

D

E

A

K

Y

A

G

E

E

E

G

N

W

W

F

I

F

V

R

V

V

L

M

T

P

D

S

V

D

D

E

Q

S

A

E

A

V

F

D

D

A

A

L

L

L

E

D

D

A

A

E11 (≠ R18) mutation to A: No effect on ADP-ribosylation.
E24 (≠ H32) mutation to A: Large decrease in ADP-ribosylation.
D27 (≠ E35) modified: ADP-ribosyl aspartic acid; mutation to A: Total loss of ADP-ribosylation.
D28 (≠ L36) mutation to A: No effect on ADP-ribosylation.
D46 (≠ G55) mutation to A: No effect on ADP-ribosylation.
D47 (= D56) mutation to A: No effect on ADP-ribosylation.
E53 (= E62) mutation to Q: Decrease in lipoylation.
K56 (= K65) modified: N6-lipoyllysine; mutation to R: Loss of lipoylation.

A0A0H3JT43 Glycine cleavage system H-like protein; GcvH-L from Staphylococcus aureus (strain Mu50 / ATCC 700699) (see paper)
29% identity, 87% coverage: 8:119/129 of query aligns to 1:110/110 of A0A0H3JT43

query
sites
A0A0H3JT43

L

M

K

K

Y

K

A

L

S

A

S

N

H

Y

E

L

W

W

I

V

R

E

K

K

E

V

E

G

D

D

G

-

S

L

Y

Y

T

V

V

F

G

S

I

M

T

T

E

P

H

E

A

L

Q

Q

E

D

L

D

L

I

G

G

D

T

M

V

V

G

F

Y

V

V

E

E

L

F

P

V

E

S

V

-

G

P

D

D

T

E

V

V

T

K

A

V

G

D

D

D

D

E

C

I

A

V

V

S

A

I

E
|
E

S

A

V

S

K
|
K

A

T

A

V

S

I

D

D

I

V

Y

Q

A

T

P

P

I

L

S

S

G

G

E

T

V

I

I

I

A

E

V

R

N

N

E

T

A

K

L

A

E

E

D

E

S

E

P

P

E

T

L

I

V

L

N

N

S

S

D

E

A

K

Y

P

G

E

E

E

G

N

W

W

F

L

F

F

R

K

V

L

M

D

P

D

S

V

D

D

E

K

S

E

E

A

V

F

D

L

A

A

L

L

L

P

D

E

A

A

E53 (= E62) mutation to Q: Decrease in lipoylation.
K56 (= K65) modified: N6-lipoyllysine; mutation to R: Loss of lipoylation.

Query Sequence

>7026358 FitnessBrowser__ANA3:7026358
MSNIPTELKYASSHEWIRKEEDGSYTVGITEHAQELLGDMVFVELPEVGDTVTAGDDCAV
AESVKAASDIYAPISGEVIAVNEALEDSPELVNSDAYGEGWFFRVMPSDESEVDALLDAE
GYQAVIDEE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory