SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 7026925 FitnessBrowser__ANA3:7026925 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5u1oB 2.3 angstrom resolution crystal structure of glutathione reductase from vibrio parahaemolyticus in complex with fad.
76% identity, 100% coverage: 1:451/451 of query aligns to 1:451/451 of 5u1oB

query
sites
5u1oB

M

M

A

A

Q

T

H

H

F

F

D

D

Y

Y

I

I

C

C

L

I

G

G

A

G

G
|
G

S
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S

G
|
G

G

G

I

I

A

A

S

S

A

A

N

N

R

R

A

A

A

A

M

M

R

Y

G

G

A

A

K

K

V

V

L

A

L

L

I

I

E
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E

A
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A

K

Q

H

D

V
x
L

G

G

G

G

T
|
T

C
|
C

V

V

N

N

V

V

G
|
G

C
|
C

V

V

P

P

K
|
K

K

K

V

V

M

M

W

W

Y

H

G

G

A

A

H

Q

I

I

A

A

E

E

A

A

M

M

N

N

L

L

Y

Y

A

A

K

E

D

D

Y

Y

G

G

F

F

D

D

V

V

S

D

V

V

N

K

K

G

F

F

D

D

W

W

N

S

T

K

L

L

V

V

N

E

S

S

R

R

E

Q

A

A

Y

Y

I

I

G

G

R

R

I

I

H

H

E

Q

A

S

Y

Y

G

D

R

R

G

V

F

L

T

G

N

N

N

N

K

K

V

V

T

N

L

V

L

I

N

K

G

G

Y
x
F

G
x
A

R

K

F

F

V

V

N

D

G

E

N

K

T

T

I

V

E

E

V

V

N

N

G

G

E

E

H

H

Y

Y

T

T

A

A

D

D

H

H

I

I

L

L

I

I

A

A

T
x
V

G

G

G

G

A

R

P

P

T

T

I

I

P

P

N

N

I

I

P

P

G

G

A

A

E

E

Y

Y

G

G

I

I

D

D

S

S

D

N

G

G

F

F

F

F

A

D

L

L

R

A

E

E

Q

Q

P

P

K

K

R

R

V

V

A

A

V

V

V

V

G

G

A

A

G

G

Y
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Y

I
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I

A

A

V

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E
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E

V

I

A

A

G

G

V

V

L

L

H

H

A

A

L

L

G

G

S

T

E

E

T

T

H

H

L

L

F

F

V

V

R

R

K

K

H

E

A

S

P

P

L

L

R

R

N

S

F

F

D

D

P

P

M

M

L

I

I

I

D

D

A

T

L

L

V

V

D

E

A

V

M

M

K

E

T

A

E

E

G

G

P

P

T

T

L

L

H

H

T

T

N

H

S

S

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V

P

P

Q

K

S

E

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V

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V

K

K

N

E

A

A

D

D

D

G

S

S

L

L

T

T

L

L

N

H

L

L

E

E

N

N

G

G

E

E

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S

V

Q

T

N

V

V

D

D

C

Q

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L

I

I

W

W

A

A

I

I

G

G

R
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R

S

H

P

P

A

A

T

T

G

D

N

A

I

I

G

N

L

L

E

A

N

S

T

T

D

G

V

V

Q

A

L

T

D

N

S

E

K

K

G

G

Y

Y

V

I

I

K

T

V

D

D

A

E

Q

Y

Q

Q

N

E

T

T

T

N

H

V

K

K

G

G

I

I

Y

Y

C

C

V

V

G
|
G

D
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D

I

I

M

M

A

E

G

G

G

G

V

I

E
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E

L
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L

T
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T

P

P

V

V

A

A

V

V

K

K

A

A

G

G

R

R

L

Q

L

L

S

S

E

E

R

R

L

L

F

F

N

N

G

N

M

K

S

P

D

N

A

A

K

K

M

M

D

D

Y

Y

S

D

Q

L

I

V

P

P

T

T

V

V

V

V

F

F

S

S

H

H

P

P

P

P

I

I

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M

I

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G

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L

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T

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Q

E

E

A

A

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E

A

E

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K

Y

Y

G

G

D

K

D

D

N

N

V

I

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K

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V

Y

Y

T

T

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S

G

G

F

F

T

T

S

A

M

M

Y

Y

T

T

A

A

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V

T

T

S

K

H

H

R

R

Q

Q

A

P

C

C

K

K

M

M

K

K

L

L

V

V

C

C

A

A

G

G

K

E

E

E

E

E

K

T

V

V

V

V

G

G

I

L

H

H

G

G

I

I

G

G

F

F

G

T

M

V

D

D

E

E

I

M

L

I

Q

Q

G

G

F

F

G

G

V

V

A

A

M

M

K

K

M

M

G

G

A

A

T

T

K

K

A

A

D

D

F

F

D

D

A

S

V

V

V

V

A
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A

I

I

H
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H

P

P

T

T

G

G

A

S

E
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E

E

E

F

F

V

V

T

T

M

M

R

R

active site: L38 (≠ V38), C42 (= C42), C47 (= C47), K50 (= K50), Y177 (= Y177), E181 (= E181), A438 (= A438), H440 (= H440), E445 (= E445)
binding flavin-adenine dinucleotide: G13 (= G13), S14 (= S14), G15 (= G15), E34 (= E34), A35 (= A35), T41 (= T41), C42 (= C42), G46 (= G46), C47 (= C47), K50 (= K50), F114 (≠ Y114), A115 (≠ G115), V139 (≠ T139), Y177 (= Y177), I178 (= I178), R263 (= R263), G302 (= G302), D303 (= D303), E310 (= E310), L311 (= L311), T312 (= T312)

5vdnA 1.55 angstrom resolution crystal structure of glutathione reductase from yersinia pestis in complex with fad
71% identity, 100% coverage: 3:451/451 of query aligns to 2:449/449 of 5vdnA

query
sites
5vdnA

Q

K

H

H

F

Y

D

D

Y

Y

I

L

C

A

L
x
I

G

G

A

G

G
|
G

S
|
S

G
|
G

G

G

I

I

A

A

S

S

A

I

N

N

R

R

A

A

A

A

M

M

R

Y

G

G

A

K

K

K

V

C

L

A

L

L

I

I

E
|
E

A
|
A

K
|
K

H

Q

V
x
L

G

G

G
|
G

T
|
T

C
|
C

V

V

N

N

V

V

G
|
G

C
|
C

V

V

P

P

K
|
K

K

K

V

V

M

M

W

W

Y

H

G

A

A

A

H

Q

I

I

A

A

E

E

A

A

M

I

N

H

L

L

Y

Y

A

G

K

P

D

D

Y

Y

G

G

F

F

D

D

V

T

S

T

V

V

N

N

K

H

F

F

D

D

W

W

N

K

T

K

L

L

V

I

N

A

S

N

R

R

E

T

A

A

Y

Y

I

I

G

D

R

R

I

I

H

H

E

Q

A

S

Y

Y

G

E

R

R

G

G

F

L

T

G

N

N

N

N

K

K

V

V

T

D

L

V

L

I

N

Q

G

G

Y
x
F

G
x
A

R

R

F

F

V

V

N

D

G

A

N

H

T

T

I

V

E

E

V

V

N

N

G

G

E

E

H

T

Y

I

T

T

A

A

D

D

H

H

I

I

L

L

I

I

A

A

T
|
T

G
|
G

G
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G

A

R

P

P

T

S

I

H

P

P

N

D

I

I

P

P

G

G

A

A

E

E

Y

Y

G

G

I

I

D

D

S

S

D
|
D

G

G

F

F

F

F

A

E

L

L

R

D

E

E

Q

M

P

P

K

K

R

R

V

V

A

A

V

V

V

V

G

G

A

A

G

G

Y
|
Y

I
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I

A

A

V

V

E
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E

V

I

A

A

G

G

V

V

L

L

H

N

A

G

L

L

G

G

S

T

E

E

T

T

H

H

L

L

F

F

V

V

R

R

K

K

H

H

A

A

P

P

L

L

R

R

N

T

F

F

D

D

P

P

M

L

L

I

I

V

D

E

A

T

L

L

V

L

D

E

A

V

M

M

K

N

T

T

E

E

G

G

P

P

T

K

L

L

H

H

T

T

N

E

S

S

V

V

P

P

Q

K

S

A

V

V

V

I

K

K

N

N

A

A

D

D

D

G

S

S

L

L

T

T

L

L

N

Q

L

L

E

E

N

N

G

G

E

T

S

E

V

V

T

T

V

V

D

D

C

H

L

L

I

I

W

W

A

A

I

I

G

G

R
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R

S

E

P

P

A

A

T

T

G

D

N

N

I

L

G

N

L

L

E

S

N

V

T

T

D

G

V

V

Q

K

L

T

D

N

S

D

K

K

G

G

Y

Y

V

I

I

E

T

V

D

D

A

K

Q

F

Q

Q

N

N

T

T

T

N

H

V

K

K

G

G

I

I

Y

Y

C

A

V

V

G
|
G

D
|
D

I

-

M

N

A

T

G

G

G

V

V

V

E
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E

L
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L

T
|
T

P

P

V

V

A

A

V

V

K

A

A

A

G

G

R

R

L

R

L

L

S

S

E

E

R

R

L

L

F

F

N

N

G

N

M

K

S

P

D

D

A

E

K

H

M

L

D

D

Y

Y

S

S

Q

N

I

I

P

P

T

T

V

V

V

V

F

F

S

S

H

H

P

P

P

P

I

I

G

G

T

T

M

I

G

G

L

L

T

T

E

E

P

P

E

Q

A

A

R

R

A

E

Q

K

Y

F

G

G

D

D

D

D

N

Q

V

V

K

K

V

V

Y

Y

T

T

S

S

G

S

F

F

T

T

S

A

M

M

Y

Y

T

S

A

A

V

V

T

T

S

Q

H

H

R

R

Q

Q

A

P

C

C

K

R

M

M

K

K

L

L

V

V

C

C

A

V

G

G

K

A

E

E

E

E

K

K

V

I

V

V

G

G

I

I

H

H

G

G

I

I

G

G

F

F

G

G

M

M

D

D

E

E

I

I

L

L

Q

Q

G

G

F

F

G

A

V

V

A

A

M

M

K

K

M

M

G

G

A

A

T

T

K

K

A

K

D

D

F

F

D

D

A

N

V

T

V

V

A
|
A

I

I

H
|
H

P

P

T

T

G

A

A

A

E
|
E

E

E

F

F

V

V

T

T

M

M

R

R

active site: L37 (≠ V38), C41 (= C42), C46 (= C47), K49 (= K50), Y176 (= Y177), E180 (= E181), A436 (= A438), H438 (= H440), E443 (= E445)
binding beta-D-fructopyranose: K35 (= K36), T40 (= T41), G140 (= G141), D157 (= D158)
binding flavin-adenine dinucleotide: I9 (≠ L10), G12 (= G13), S13 (= S14), G14 (= G15), E33 (= E34), A34 (= A35), G39 (= G40), T40 (= T41), C41 (= C42), G45 (= G46), C46 (= C47), K49 (= K50), F113 (≠ Y114), A114 (≠ G115), T138 (= T139), G139 (= G140), Y176 (= Y177), I177 (= I178), R262 (= R263), G301 (= G302), D302 (= D303), E308 (= E310), L309 (= L311), T310 (= T312)

P06715 Glutathione reductase; GR; GRase; EC 1.8.1.7 from Escherichia coli (strain K12) (see paper)
69% identity, 100% coverage: 1:451/451 of query aligns to 1:450/450 of P06715

query
sites
P06715

M

M

A

T

Q

K

H

H

F

Y

D

D

Y

Y

I

I

C

A

L

I

G

G

A

G

G

G

S

S

G

G

G

G

I

I

A

A

S

S

A

I

N

N

R

R

A

A

A

A

M

M

R

Y

G

G

A

Q

K

K

V

C

L

A

L

L

I

I

E

E

A

A

K

K

H

E

V

L

G

G

G

G

T

T

C
|
C

V

V

N

N

V

V

G

G

C
|
C

V

V

P

P

K

K

K

K

V

V

M

M

W

W

Y

H

G

A

A

A

H

Q

I

I

A

R

E

E

A

A

M

I

N

H

L

M

Y

Y

A

G

K

P

D

D

Y

Y

G

G

F

F

D

D

V

T

S

T

V

I

N

N

K

K

F

F

D

N

W

W

N

E

T

T

L

L

V

I

N

A

S

S

R

R

E

T

A

A

Y

Y

I

I

G

D

R

R

I

I

H

H

E

T

A

S

Y

Y

G

E

R

N

G

V

F

L

T

G

N

K

N

N

K

N

V

V

T

D

L

V

L

I

N

K

G

G

Y

F

G

A

R

R

F

F

V

V

N

D

G

A

N

K

T

T

I

L

E

E

V

V

N

N

G

G

E

E

H

T

Y

I

T

T

A

A

D

D

H

H

I

I

L

L

I

I

A

A

T

T

G

G

G

G

A

R

P

P

T

S

I

H

P

P

N

D

I

I

P

P

G

G

A

V

E

E

Y

Y

G

G

I

I

D

D

S

S

D

D

G

G

F

F

F

F

A

A

L

L

R

P

E

A

Q

L

P

P

K

E

R

R

V

V

A

A

V

V

V

V

G

G

A

A

G

G

Y

Y

I

I

A

A

V

V

E

E

V

L

A

A

G

G

V

V

L

I

H

N

A

G

L

L

G

G

S

A

E

K

T

T

H

H

L

L

F

F

V

V

R

R

K

K

H

H

A

A

P

P

L

L

R

R

N

S

F

F

D

D

P

P

M

M

L

I

I

S

D

E

A

T

L

L

V

V

D

E

A

V

M

M

K

N

T

A

E

E

G

G

P

P

T

Q

L

L

H

H

T

T

N

N

S

A

V

I

P

P

Q

K

S

A

V

V

V

V

K

K

N

N

A

T

D

D

D

G

S

S

L

L

T

T

L

L

N

E

L

L

E

E

N

D

G

G

E

R

S

S

V

E

T

T

V

V

D

D

C

C

L

L

I

I

W

W

A

A

I

I

G

G

R

R

S

E

P

P

A

A

T

N

G

D

N

N

I

I

G

N

L

L

E

E

N

A

T

A

D

G

V

V

Q

K

L

T

D

N

S

E

K

K

G

G

Y

Y

V

I

I

V

T

V

D

D

A

K

Q

Y

Q

Q

N

N

T

T

T

N

H

I

K

E

G

G

I

I

Y

Y

C

A

V

V

G

G

D

D

I

-

M

N

A

T

G

G

G

A

V

V

E

E

L

L

T

T

P

P

V

V

A

A

V

V

K

A

A

A

G

G

R

R

L

R

L

L

S

S

E

E

R

R

L

L

F

F

N

N

G

N

M

K

S

P

D

D

A

E

K

H

M

L

D

D

Y

Y

S

S

Q

N

I

I

P

P

T

T

V

V

V

V

F

F

S

S

H

H

P

P

P

P

I

I

G

G

T

T

M

V

G

G

L

L

T

T

E

E

P

P

E

Q

A

A

R

R

A

E

Q

Q

Y

Y

G

G

D

D

D

D

N

Q

V

V

K

K

V

V

Y

Y

T

K

S

S

G

S

F

F

T

T

S

A

M

M

Y

Y

T

T

A

A

V

V

T

T

S

T

H

H

R

R

Q

Q

A

P

C

C

K

R

M

M

K

K

L

L

V

V

C

C

A

V

G

G

K

S

E

E

E

E

K

K

V

I

V

V

G

G

I

I

H

H

G

G

I

I

G

G

F

F

G

G

M

M

D

D

E

E

I

M

L

L

Q

Q

G

G

F

F

G

A

V

V

A

A

M

L

K

K

M

M

G

G

A

A

T

T

K

K

A

K

D

D

F

F

D

D

A

N

V

T

V

V

A

A

I

I

H

H

P

P

T

T

G

A

A

A

E

E

E

E

F

F

V

V

T

T

M

M

R

R

C42 (= C42) modified: Disulfide link with 47, Redox-active
C47 (= C47) modified: Disulfide link with 42, Redox-active

1gerB The structure of glutathione reductase from escherichia coli at 1.86 angstroms resolution: comparison with the enzyme from human erythrocytes (see paper)
69% identity, 100% coverage: 3:451/451 of query aligns to 2:449/449 of 1gerB

query
sites
1gerB

Q

K

H

H

F

Y

D

D

Y

Y

I

I

C

A

L
x
I

G
|
G

A

G

G
|
G

S
|
S

G
|
G

G

G

I

I

A

A

S

S

A

I

N

N

R

R

A

A

A

A

M

M

R

Y

G

G

A

Q

K

K

V

C

L

A

L

L

I

I

E
|
E

A
|
A

K

K

H

E

V
x
L

G

G

G
|
G

T
|
T

C
|
C

V

V

N

N

V

V

G
|
G

C
|
C

V

V

P

P

K
|
K

K

K

V

V

M

M

W

W

Y

H

G

A

A

A

H

Q

I

I

A

R

E

E

A

A

M

I

N

H

L

M

Y

Y

A

G

K

P

D

D

Y

Y

G

G

F

F

D

D

V

T

S

T

V

I

N

N

K

K

F

F

D

N

W

W

N

E

T

T

L

L

V

I

N

A

S

S

R

R

E

T

A

A

Y

Y

I

I

G

D

R

R

I

I

H

H

E

T

A

S

Y

Y

G

E

R

N

G

V

F

L

T

G

N

K

N

N

K

N

V

V

T

D

L

V

L

I

N

K

G

G

Y
x
F

G
x
A

R

R

F

F

V

V

N

D

G

A

N

K

T

T

I

L

E

E

V

V

N

N

G

G

E

E

H

T

Y

I

T

T

A

A

D

D

H

H

I

I

L

L

I

I

A

A

T
|
T

G

G

G

G

A

R

P

P

T

S

I

H

P

P

N

D

I

I

P

P

G

G

A

V

E

E

Y

Y

G

G

I

I

D

D

S

S

D

D

G

G

F

F

F

F

A

A

L

L

R

P

E

A

Q

L

P

P

K

E

R

R

V

V

A

A

V

V

V

V

G

G

A

A

G

G

Y
|
Y

I
|
I

A

A

V

V

E
|
E

V

L

A

A

G

G

V

V

L

I

H

N

A

G

L

L

G

G

S

A

E

K

T

T

H

H

L

L

F

F

V

V

R

R

K

K

H

H

A

A

P

P

L

L

R

R

N

S

F

F

D

D

P

P

M

M

L

I

I

S

D

E

A

T

L

L

V

V

D

E

A

V

M

M

K

N

T

A

E

E

G

G

P

P

T

Q

L

L

H

H

T

T

N

N

S

A

V

I

P

P

Q

K

S

A

V

V

V

V

K

K

N

N

A

T

D

D

D

G

S

S

L

L

T

T

L

L

N

E

L

L

E

E

N

D

G

G

E

R

S

S

V

E

T

T

V

V

D

D

C

C

L

L

I

I

W

W

A

A

I

I

G

G

R
|
R

S

E

P

P

A

A

T

N

G

D

N

N

I

I

G

N

L

L

E

E

N

A

T

A

D

G

V

V

Q

K

L

T

D

N

S

E

K

K

G

G

Y

Y

V

I

I

V

T

V

D

D

A

K

Q

Y

Q

Q

N

N

T

T

T

N

H

I

K

E

G

G

I

I

Y

Y

C

A

V

V

G
|
G

D
|
D

I

-

M

N

A

T

G

G

G

A

V

V

E
|
E

L
|
L

T
|
T

P

P

V

V

A

A

V

V

K

A

A

A

G

G

R

R

L

R

L

L

S

S

E

E

R

R

L

L

F

F

N

N

G

N

M

K

S

P

D

D

A

E

K

H

M

L

D

D

Y

Y

S

S

Q

N

I

I

P

P

T

T

V

V

V

V

F

F

S

S

H

H

P

P

P

P

I

I

G

G

T

T

M

V

G

G

L

L

T

T

E

E

P

P

E

Q

A

A

R

R

A

E

Q

Q

Y

Y

G

G

D

D

D

D

N

Q

V

V

K

K

V

V

Y

Y

T

K

S

S

G

S

F

F

T

T

S

A

M

M

Y

Y

T

T

A

A

V

V

T

T

S

T

H

H

R

R

Q

Q

A

P

C

C

K

R

M

M

K

K

L

L

V

V

C

C

A

V

G

G

K

S

E

E

E

E

K

K

V

I

V

V

G

G

I

I

H

H

G

G

I

I

G

G

F

F

G

G

M

M

D

D

E

E

I

M

L

L

Q

Q

G

G

F

F

G

A

V

V

A

A

M

L

K

K

M

M

G

G

A

A

T

T

K

K

A

K

D

D

F

F

D

D

A

N

V

T

V

V

A
|
A

I

I

H
|
H

P

P

T

T

G

A

A

A

E
|
E

E

E

F

F

V

V

T

T

M

M

R

R

active site: L37 (≠ V38), C41 (= C42), C46 (= C47), K49 (= K50), Y176 (= Y177), E180 (= E181), A436 (= A438), H438 (= H440), E443 (= E445)
binding flavin-adenine dinucleotide: I9 (≠ L10), G10 (= G11), G12 (= G13), S13 (= S14), G14 (= G15), E33 (= E34), A34 (= A35), G39 (= G40), T40 (= T41), C41 (= C42), G45 (= G46), C46 (= C47), K49 (= K50), F113 (≠ Y114), A114 (≠ G115), T138 (= T139), Y176 (= Y177), I177 (= I178), R262 (= R263), G301 (= G302), D302 (= D303), E308 (= E310), L309 (= L311), T310 (= T312)

1getA Anatomy of an engineered NAD-binding site (see paper)
69% identity, 100% coverage: 3:451/451 of query aligns to 1:448/448 of 1getA

query
sites
1getA

Q

K

H

H

F

Y

D

D

Y

Y

I

I

C

A

L
x
I

G
|
G

A

G

G
|
G

S
|
S

G

G

G

G

I

I

A

A

S

S

A

I

N

N

R

R

A

A

A

A

M

M

R

Y

G

G

A

Q

K

K

V

C

L

A

L

L

I

I

E
|
E

A
|
A

K

K

H

E

V
x
L

G

G

G
|
G

T
|
T

C
|
C

V

V

N

N

V

V

G
|
G

C
|
C

V

V

P

P

K
|
K

K

K

V

V

M

M

W

W

Y

H

G

A

A

A

H

Q

I

I

A

R

E

E

A

A

M

I

N

H

L

M

Y

Y

A

G

K

P

D

D

Y

Y

G

G

F

F

D

D

V

T

S

T

V

I

N

N

K

K

F

F

D

N

W

W

N

E

T

T

L

L

V

I

N

A

S

S

R

R

E

T

A

A

Y

Y

I

I

G

D

R

R

I

I

H

H

E

T

A

S

Y

Y

G

E

R

N

G

V

F

L

T

G

N

K

N

N

K

N

V

V

T

D

L

V

L

I

N

K

G

G

Y
x
F

G
x
A

R

R

F

F

V

V

N

D

G

A

N

K

T

T

I

L

E

E

V

V

N

N

G

G

E

E

H

T

Y

I

T

T

A

A

D

D

H

H

I

I

L

L

I

I

A

A

T
|
T

G

G

G

G

A

R

P

P

T

S

I

H

P

P

N

D

I

I

P

P

G

G

A

V

E

E

Y

Y

G

G

I

I

D

D

S

S

D

D

G

G

F

F

F

F

A

A

L

L

R

P

E

A

Q

L

P

P

K

E

R

R

V

V

A

A

V

V

V

V

G

G

A
|
A

G
|
G

Y
|
Y

I
|
I

A

A

V

V

E
|
E

V

L

A

A

G

G

V

V

L

I

H

N

A

G

L

L

G

G

S

A

E

K

T

T

H

H

L

L

F

F

V

V

R
|
R

K

K

H

H

A

A

P

P

L

L

R
|
R

N

S

F

F

D

D

P

P

M

M

L

I

I

S

D

E

A

T

L

L

V

V

D

E

A

V

M

M

K

N

T

A

E

E

G

G

P

P

T

Q

L

L

H

H

T

T

N

N

S

A

V

I

P

P

Q

K

S

A

V

V

V

V

K

K

N

N

A

T

D

D

D

G

S

S

L

L

T

T

L

L

N

E

L

L

E

E

N

D

G

G

E

R

S

S

V

E

T

T

V

V

D

D

C

C

L

L

I

I

W

W

A

A

I
|
I

G
|
G

R
|
R

S

E

P

P

A

A

T

N

G

D

N

N

I

I

G

N

L

L

E

E

N

A

T

A

D

G

V

V

Q

K

L

T

D

N

S

E

K

K

G

G

Y

Y

V

I

I

V

T

V

D

D

A

K

Q

Y

Q

Q

N

N

T

T

T

N

H

I

K

E

G

G

I

I

Y

Y

C

A

V

V

G
|
G

D
|
D

I

-

M

N

A

T

G

G

G

A

V

V

E
|
E

L
|
L

T
|
T

P

P

V

V

A

A

V

V

K

A

A

A

G

G

R

R

L

R

L

L

S

S

E

E

R

R

L

L

F

F

N

N

G

N

M

K

S

P

D

D

A

E

K

H

M

L

D

D

Y

Y

S

S

Q

N

I

I

P

P

T

T

V
|
V

V

V

F

F

S

S

H

H

P

P

P

P

I

I

G

G

T

T

M

V

G

G

L

L

T

T

E

E

P

P

E

Q

A

A

R

R

A

E

Q

Q

Y

Y

G

G

D

D

D

D

N

Q

V

V

K

K

V

V

Y

Y

T

K

S

S

G

S

F

F

T

T

S

A

M

M

Y

Y

T

T

A

A

V

V

T

T

S

T

H

H

R

R

Q

Q

A

P

C

C

K

R

M

M

K

K

L

L

V

V

C

C

A

V

G

G

K

S

E

E

E

E

K

K

V

I

V

V

G

G

I

I

H

H

G

G

I

I

G

G

F

F

G

G

M

M

D

D

E

E

I

M

L

L

Q

Q

G

G

F

F

G

A

V

V

A

A

M

L

K

K

M

M

G

G

A

A

T

T

K

K

A

K

D

D

F

F

D

D

A

N

V

T

V

V

A
|
A

I

I

H
|
H

P

P

T

T

G

A

A

A

E
|
E

E

E

F

F

V

V

T

T

M

M

R

R

active site: L36 (≠ V38), C40 (= C42), C45 (= C47), K48 (= K50), Y175 (= Y177), E179 (= E181), A435 (= A438), H437 (= H440), E442 (= E445)
binding flavin-adenine dinucleotide: I8 (≠ L10), G9 (= G11), G11 (= G13), S12 (= S14), E32 (= E34), A33 (= A35), G38 (= G40), T39 (= T41), C40 (= C42), G44 (= G46), C45 (= C47), K48 (= K50), F112 (≠ Y114), A113 (≠ G115), T137 (= T139), I176 (= I178), R261 (= R263), G300 (= G302), D301 (= D303), E307 (= E310), L308 (= L311), T309 (= T312)
binding nadp nicotinamide-adenine-dinucleotide phosphate: A173 (= A175), G174 (= G176), Y175 (= Y177), I176 (= I178), E179 (= E181), R196 (= R198), R202 (= R204), I259 (= I261), G260 (= G262), E307 (= E310), L308 (= L311), V340 (= V343)

1geuA Anatomy of an engineered NAD-binding site (see paper)
67% identity, 100% coverage: 3:451/451 of query aligns to 1:448/448 of 1geuA

query
sites
1geuA

Q

K

H

H

F

Y

D

D

Y

Y

I

I

C

A

L

I

G
|
G

A

G

G
|
G

S
|
S

G
|
G

G

G

I

I

A

A

S

S

A

I

N

N

R

R

A

A

A

A

M

M

R

Y

G

G

A

Q

K

K

V

C

L

A

L

L

I

I

E
|
E

A
|
A

K
|
K

H

E

V
x
L

G

G

G
|
G

T
|
T

C
|
C

V

V

N

N

V

V

G
|
G

C
|
C

V

V

P

P

K
|
K

K

K

V

V

M

M

W

W

Y

H

G

A

A

A

H

Q

I

I

A

R

E

E

A

A

M

I

N

H

L

M

Y

Y

A

G

K

P

D

D

Y

Y

G

G

F

F

D

D

V

T

S

T

V

I

N

N

K

K

F

F

D

N

W

W

N

E

T

T

L

L

V

I

N

A

S

S

R

R

E

T

A

A

Y

Y

I

I

G

D

R

R

I

I

H

H

E

T

A

S

Y

Y

G

E

R

N

G

V

F

L

T

G

N

K

N

N

K

N

V

V

T

D

L

V

L

I

N

K

G

G

Y
x
F

G
x
A

R

R

F

F

V

V

N

D

G

A

N

K

T

T

I

L

E

E

V

V

N

N

G

G

E

E

H

T

Y

I

T

T

A

A

D

D

H

H

I

I

L

L

I

I

A

A

T
|
T

G

G

G

G

A

R

P

P

T

S

I

H

P

P

N

D

I

I

P

P

G

G

A

V

E

E

Y

Y

G

G

I

I

D

D

S

S

D

D

G

G

F

F

F

F

A

A

L

L

R

P

E

A

Q

L

P

P

K

E

R

R

V

V

A

A

V

V

V

V

G

G

A

A

G

G

Y
|
Y

I
|
I

A

G

V

V

E
|
E

V

L

A

G

G

G

V

V

L

I

H

N

A

G

L

L

G

G

S

A

E

K

T

T

H

H

L

L

F

F

V
x
E

R
x
M

K
x
F

H

D

A

A

P

P

L

L

R

P

N

S

F

F

D

D

P

P

M

M

L

I

I

S

D

E

A

T

L

L

V

V

D

E

A

V

M

M

K

N

T

A

E

E

G

G

P

P

T

Q

L

L

H

H

T

T

N

N

S

A

V

I

P

P

Q

K

S

A

V

V

V

V

K

K

N

N

A

T

D

D

D

G

S

S

L

L

T

T

L

L

N

E

L

L

E

E

N

D

G

G

E

R

S

S

V

E

T

T

V

V

D

D

C

C

L

L

I

I

W

W

A
|
A

I
|
I

G
|
G

R

R

S

E

P

P

A

A

T

N

G

D

N

N

I

I

G

N

L

L

E

E

N

A

T

A

D

G

V

V

Q

K

L

T

D

N

S

E

K

K

G

G

Y

Y

V

I

I

V

T

V

D

D

A

K

Q

Y

Q

Q

N

N

T

T

T

N

H

I

K

E

G

G

I

I

Y

Y

C

A

V

V

G
|
G

D
|
D

I

-

M

N

A

T

G

G

G

A

V

V

E
|
E

L
|
L

T
|
T

P

P

V

V

A

A

V

V

K

A

A

A

G

G

R

R

L

R

L

L

S

S

E

E

R

R

L

L

F

F

N

N

G

N

M

K

S

P

D

D

A

E

K

H

M

L

D

D

Y

Y

S

S

Q

N

I

I

P

P

T

T

V
|
V

V

V

F

F

S

S

H

H

P

P

P

P

I

I

G

G

T

T

M

V

G

G

L

L

T

T

E

E

P

P

E

Q

A

A

R

R

A

E

Q

Q

Y

Y

G

G

D

D

D

D

N

Q

V

V

K

K

V

V

Y

Y

T

K

S

S

G

S

F

F

T

T

S

A

M

M

Y

Y

T

T

A

A

V

V

T

T

S

T

H

H

R

R

Q

Q

A

P

C

C

K

R

M

M

K

K

L

L

V

V

C

C

A

V

G

G

K

S

E

E

E

E

K

K

V

I

V

V

G

G

I

I

H

H

G

G

I

I

G

G

F

F

G

G

M

M

D

D

E

E

I

M

L

L

Q

Q

G

G

F

F

G

A

V

V

A

A

M

L

K

K

M

M

G

G

A

A

T

T

K

K

A

K

D

D

F

F

D

D

A

N

V

T

V

V

A
|
A

I

I

H
|
H

P

P

T

T

G

A

A

A

E
|
E

E

E

F

F

V

V

T

T

M

M

R

R

active site: L36 (≠ V38), C40 (= C42), C45 (= C47), K48 (= K50), Y175 (= Y177), E179 (= E181), A435 (= A438), H437 (= H440), E442 (= E445)
binding flavin-adenine dinucleotide: G9 (= G11), G11 (= G13), S12 (= S14), G13 (= G15), E32 (= E34), A33 (= A35), K34 (= K36), G38 (= G40), T39 (= T41), C40 (= C42), G44 (= G46), C45 (= C47), K48 (= K50), F112 (≠ Y114), A113 (≠ G115), T137 (= T139), G300 (= G302), D301 (= D303), L308 (= L311), T309 (= T312)
binding nicotinamide-adenine-dinucleotide: Y175 (= Y177), I176 (= I178), E179 (= E181), E195 (≠ V197), M196 (≠ R198), F197 (≠ K199), A258 (= A260), I259 (= I261), G260 (= G262), E307 (= E310), L308 (= L311), V340 (= V343)

5v36A 1.88 angstrom resolution crystal structure of glutathione reductase from streptococcus mutans ua159 in complex with fad
66% identity, 100% coverage: 1:451/451 of query aligns to 2:451/451 of 5v36A

query
sites
5v36A

M

M

A

T

Q

K

H

Q

F

Y

D

D

Y

Y

I

I

C

V

L
x
I

G

G

A

G

G
|
G

S
|
S

G
|
G

G

G

I

I

A

A

S

S

A

A

N

N

R

R

A

A

A

A

M

M

R

H

G

G

A

A

K

K

V

V

L

I

L

L

I

F

E
|
E

A
x
G

K

K

H

Q

V
|
V

G

G

G
|
G

T
|
T

C
|
C

V

V

N

N

V

V

G
|
G

C
|
C

V

V

P

P

K
|
K

K

K

V

V

M

M

W

W

Y

Y

G

G

A

A

H

Q

I

V

A

A

E

E

A

T

M

I

N

N

L

N

Y

Y

A

A

K

A

D

D

Y

Y

G

G

F

F

D

D

V

V

S

T

V

T

N

Q

K

A

F

F

D

H

W

F

N

D

T

V

L

L

V

K

N

Q

S

N

R

R

E

Q

A

A

Y

Y

I

I

G

D

R

R

I

I

H

H

E

D

A

S

Y

Y

G

E

R

R

G

G

F

F

T

D

N

S

N

N

K

G

V

V

T

E

L

R

L

V

N

Y

G

G

Y
|
Y

G
x
A

R

T

F

F

V

V

N

D

G

A

N

H

T

T

I

V

E

E

V

V

N

A

G

G

E

E

H

H

Y

Y

T

T

A

A

D

P

H

H

I

I

L

L

I

I

A

A

T
|
T

G
|
G

G

G

A

H

P

A

T

L

I

L

P

P

N

D

I

I

P

P

G

G

A

S

E

E

Y

Y

G

G

I

I

D

T

S

S

D

D

G

G

F

F

F

F

A

E

L

L

R

D

E

A

Q

I

P

P

K

K

R

R

V

T

A

A

V

V

V

V

G

G

A

A

G

G

Y
|
Y

I
|
I

A

A

V

V

E
|
E

V

I

A

S

G

G

V

I

L

L

H

H

A

A

L

L

G

G

S

S

E

E

T

T

H

H

L

L

F

F

V

V

R

R

K

R

H

D

A

R

P

P

L

L

R

R

N

K

F

F

D

D

P

K

M

E

L

I

I

V

D

G

A

T

L

L

V

V

D

D

A

E

M

M

K

K

T

K

E

D

G

G

P

P

T

H

L

L

H

H

T

T

N

F

S

S

V

V

P

P

Q

K

S

E

V

V

V

I

K

K

N

N

A

T

D

D

D

N

S

S

L

L

T

T

L

L

N

I

L

L

E

E

N

N

G

G

E

E

S

E

V

Y

T

T

V

V

D

D

C

T

L

L

I

I

W

W

A

A

I

I

G

G

R
|
R

S

A

P

A

A

N

T

T

G

K

N

G

I
x
F

G

N

L

L

E

E

N

V

T

T

D

G

V

V

Q

T

L

L

D

D

S

S

K

R

G

G

Y

F

V

I

I

A

T

T

D

D

A

A

Q

F

Q

E

N

N

T

T

T

N

H

V

K

E

G

G

I

L

Y

Y

C

A

V

L

G
|
G

D
|
D

I

V

M

-

A

N

G

G

G

K

V

L

E

E

L
|
L

T
|
T

P

P

V

V

A

A

V

V

K

K

A

A

G

G

R

R

L

Q

L

L

S

S

E

E

R

R

L

L

F

F

N

N

G

H

M

K

S

P

D

Q

A

A

K

K

M

M

D

D

Y

Y

S

K

Q

D

I

V

P

A

T

T

V

V

V

I

F

F

S

S

H

H

P

P

P

V

I

I

G

G

T

S

M

I

G

G

L

L

T

S

E

E

P

E

E

A

A

A

R

L

A

D

Q

Q

Y

Y

G

G

D

E

D

E

N

N

V

V

K

T

V

V

Y

Y

T

R

S

S

G

T

F

F

T

T

S

S

M

M

Y

Y

T

T

A

A

V

V

T

T

S

S

H

H

R

R

Q

Q

A

A

C

C

K

K

M

M

K

K

L

L

V

V

C

T

A

V

G

G

K

E

E

D

E

E

K

K

V

I

V

V

G

G

I

L

H

H

G

G

I

I

G

G

F
x
Y

G
|
G

M

V

D
|
D

E

E

I

M

L

I

Q

Q

G

G

F

F

G

A

V

V

A

A

M

I

K

K

M

M

G

G

A

A

T

T

K

K

A

A

D

D

F

F

D

D

A

N

V

T

V

V

A
|
A

I

I

H
|
H

P

P

T

T

G

G

A

S

E
|
E

E

E

F

F

V

V

T

T

M

M

R

R

active site: V39 (= V38), C43 (= C42), C48 (= C47), K51 (= K50), Y178 (= Y177), E182 (= E181), A438 (= A438), H440 (= H440), E445 (= E445)
binding beta-D-fructopyranose: Y411 (≠ F411), G412 (= G412), D414 (= D414)
binding flavin-adenine dinucleotide: I11 (≠ L10), G14 (= G13), S15 (= S14), G16 (= G15), E35 (= E34), G36 (≠ A35), G41 (= G40), T42 (= T41), C43 (= C42), G47 (= G46), C48 (= C47), K51 (= K50), Y115 (= Y114), A116 (≠ G115), T140 (= T139), G141 (= G140), Y178 (= Y177), I179 (= I178), R264 (= R263), F271 (≠ I270), G303 (= G302), D304 (= D303), L311 (= L311), T312 (= T312)

6n7fA 1.90 angstrom resolution crystal structure of glutathione reductase from streptococcus pyogenes in complex with fad.
62% identity, 100% coverage: 1:451/451 of query aligns to 2:451/451 of 6n7fA

query
sites
6n7fA

M

M

A

V

Q

I

H

P

F

Y

D

D

Y

Y

I

I

C

V

L
x
I

G
|
G

A

G

G
|
G

S
|
S

G
x
A

G

G

I

I

A

A

S

S

A

A

N

N

R

R

A

A

A

A

M

M

R

H

G

G

A

A

K

K

V

V

L

L

L

L

I
x
A

E
|
E

A
x
G

K
|
K

H

E

V

I

G

G

G
|
G

T
|
T

C
|
C

V

V

N

N

V

L

G
|
G

C
|
C

V

V

P

P

K
|
K

K

K

V

V

M

M

W

W

Y

Y

G

G

A

A

H

Q

I

V

A

A

E

D

A

I

M

L

N

G

L

T

Y

Y

A

A

K

K

D

D

Y

Y

G

G

F

F

D

D

V

F

S

K

V

E

N

K

K

A

F

F

D

D

W

F

N

K

T

Q

L

L

V

K

N

A

S

N

R

R

E

Q

A

A

Y

Y

I

I

G

D

R

R

I

I

H

H

E

A

A

S

Y

Y

G

E

R

R

G

G

F

F

T

E

N

Q

N

N

K

G

V

V

T

D

L

R

L

I

N

Y

G

D

Y
|
Y

G
x
A

R

V

F

F

V

K

N

D

G

A

N

H

T

T

I

V

E

E

V

I

N

A

G

G

E

Q

H

L

Y

Y

T

T

A

A

D

P

H

H

I

I

L

L

I

I

A

A

T
|
T

G
|
G

G

G

A

H

P

P

T

V

I

F

P

P

N

D

I

I

P

E

G

G

A

A

E

Q

Y

Y

G

G

I

I

D

S

S

S

D

D

G

G

F

F

F

F

A

A

L

L

R

D

E

E

Q

V

P

P

K

K

R

R

V

T

A

A

V

V

V

V

G

G

A

A

G

G

Y
|
Y

I
|
I

A

A

V

V

E
|
E

V

L

A

A

G

G

V

V

L

L

H

H

A

A

L

L

G

G

S

S

E

K

T

T

H

D

L

L

F

F

V

I

R

R

K

H

H

D

A

R

P

P

L

L

R

R

N

S

F

F

D

D

P

K

M

T

L

I

I

V

D

D

A

V

L

L

V

V

D

D

A

E

M

M

K

A

T

V

E

N

G

G

P

P

T

R

L

L

H

H

T

T

N

H

S

A

V

E

P

V

Q

A

S

K

V

V

V

V

K

K

N

N

A

T

D

D

D

E

S

S

L

L

T

T

L

L

N

Y

L

L

E

K

N

D

G

G

E

Q

S

E

V

V

T

E

V

V

D

D

C

Q

L

L

I

I

W

W

A

A

I

I

G

G

R
|
R

S

K

P

P

A

N

T

L

G

E

N
x
G

I

F

G

S

L

L

E

D

N

K

T

T

D

G

V

V

Q

T

L

L

D

N

S

D

K

K

G

G

Y

Y

V

I

I

E

T

T

D

D

A

A

Q

Y

Q

E

N

N

T

T

T

S

H

V

K

K

G

G

I

I

Y

Y

C

A

V

V

G
|
G

D
|
D

I

V

M

-

A

N

G

G

G

K

V

L

E

A

L
|
L

T
|
T

P

P

V

V

A

A

V

V

K

A

A

A

G

G

R

R

L

R

L

L

S

S

E

E

R

R

L

L

F

F

N

N

G

G

M

K

S

T

D

D

A

E

K

K

M

L

D

D

Y

Y

S

Q

Q

N

I

V

P

A

T

T

V

V

V

I

F

F

S

S

H

H

P

P

P

V

I

I

G

G

T

S

M

V

G

G

L

L

T

S

E

E

P

E

E

A

A

A

R

V

A

K

Q

Q

Y

Y

G

G

D

Q

D

E

N

A

V

V

K

K

V

T

Y

Y

T

Q

S

S

G

R

F

F

T

T

S

S

M

M

Y

F

T

T

A

A

V

I

T

T

S

N

H

H

R

R

Q

Q

A

P

C

C

K

L

M

M

K

K

L

L

V

V

C

T

A

V

G

G

K

D

E

T

E

E

K

K

V

I

V

V

G

G

I

L

H

H

G

G

I

I

G

G

F

Y

G

G

M

V

D

D

E

E

I

M

L

I

Q

Q

G

G

F

F

G

A

V

V

A

A

M

I

K

K

M

M

G

G

A

A

T

T

K

K

A

A

D

D

F

F

D

D

A

N

V

T

V

V

A

A

I

I

H
|
H

P

P

T

T

G

G

A

S

E
|
E

E

E

F

F

V

V

T

T

M

M

R

R

active site: C43 (= C42), C48 (= C47), K51 (= K50), Y178 (= Y177), E182 (= E181), H440 (= H440), E445 (= E445)
binding flavin-adenine dinucleotide: I11 (≠ L10), G12 (= G11), G14 (= G13), S15 (= S14), A16 (≠ G15), A34 (≠ I33), E35 (= E34), G36 (≠ A35), K37 (= K36), G41 (= G40), T42 (= T41), C43 (= C42), G47 (= G46), C48 (= C47), K51 (= K50), Y115 (= Y114), A116 (≠ G115), T140 (= T139), G141 (= G140), Y178 (= Y177), I179 (= I178), R264 (= R263), G303 (= G302), D304 (= D303), L311 (= L311), T312 (= T312)
binding riboflavin: G36 (≠ A35), K37 (= K36), Y115 (= Y114), G270 (≠ N269)

6b4oA 1.73 angstrom resolution crystal structure of glutathione reductase from enterococcus faecalis in complex with fad
58% identity, 99% coverage: 5:451/451 of query aligns to 6:451/451 of 6b4oA

query
sites
6b4oA

F

Y

D

D

Y

Y

I

I

C

V

L
x
I

G

G

A

G

G
|
G

S
|
S

G
|
G

G

G

I

I

A

A

S

S

A

A

N

N

R

R

A

A

A

G

M

M

R

H

G

G

A

A

K

N

V

V

L

L

L

L

I

I

E
|
E

A
x
G

K

N

H

E

V
x
I

G

G

G
|
G

T
|
T

C
|
C

V

V

N

N

V

V

G
|
G

C
|
C

V

V

P

P

K
|
K

K

K

V

V

M

M

W

W

Y

Q

G

A

A

S

H

S

I

M

A

M

E

E

A

M

M

M

N

E

L

R

Y

D

A

T

K

A

D

G

Y

Y

G

G

F

F

D

D

V

V

S

E

V

I

N

K

K

N

F

F

D

S

W

F

N

K

T

Q

L

L

V

V

N

E

S

N

R

R

E

E

A

K

Y

Y

I

I

G

D

R

F

I

L

H

H

E

G

A

A

Y

Y

G

N

R

R

G

G

F

L

T

D

N

S

N

N

K

N

V

I

T

E

L

R

L

I

N

H

G

G

Y
|
Y

G
x
A

R

T

F

F

V

T

N

G

G

E

N

Q

T

T

I

I

E

E

V

V

N

N

G

G

E

T

H

E

Y

Y

T

T

A

A

D

P

H

H

I

I

L

L

I

I

A

A

T
|
T

G
|
G

G

G

A

R

P

P

T

K

I

K

P

L

N

G

I

I

P

P

G

G

A

E

E

E

Y

Y

G

A

I

L

D

D

S

S

D

N

G

G

F

F

F

F

A

A

L

L

R

E

E

E

Q

M

P

P

K

K

R

R

V

V

A

V

V

F

V

V

G

G

A

A

G

G

Y
|
Y

I
|
I

A

A

V

A

E
|
E

V

L

A

A

G

G

V

T

L

L

H

H

A

G

L

L

G

G

S

A

E

E

T

T

H

H

L

W

F

A

V

F

R

R

K

H

H

E

A

R

P

P

L

L

R

R

N

S

F

F

D

D

P

D

M

M

L

L

I

S

D

E

A

K

L

V

V

V

D

E

A

R

M

Y

K

Q

T

E

E

M

G

G

P

M

T

Q

L

I

H

H

T

P

N

N

S

A

V

T

P

P

Q

A

S

K

V

I

V

E

K

K

N

T

A

A

D

Q

D

N

S

E

L

Y

T

V

L

I

N

T

L

F

E

E

N

N

G

G

E

E

S

S

V

I

T

T

V

T

D

D

C

A

L

V

I

I

W

F

A

G

I

T

G

G

R

R

S

Q

P

P

A

N

T

T

G

D

N

Q

I

L

G

G

L

L

E

E

N

N

T

T

D

K

V

V

Q

A

L

L

D

D

S

E

K

K

G

G

Y

Y

V

V

I

K

T

V

D

D

A

K

Q

F

Q

Q

N

N

T

T

T

T

H

Q

K

N

G

G

I

I

Y

Y

C

A

V

V

G
|
G

D
|
D

I

V

M

I

A

-

G

G

G

K

V

I

E
x
D

L
|
L

T
|
T

P

P

V

V

A

A

V

I

K

A

A

A

G

G

R

R

L

R

L

L

S

S

E

E

R

R

L

L

F

F

N

N

G

G

M

Q

S

T

D

D

A

L

K

Y

M

L

D

D

Y

Y

S

N

Q

L

I

V

P

P

T

T

V

V

V

V

F

F

S

T

H

H

P

P

P

P

I

V

G

A

T

T

M

I

G

G

L

L

T

T

E

E

P

K

E

A

A

A

R

L

A

E

Q

E

Y

Y

G

G

D

E

D

D

N

Q

V

V

K

K

V

I

Y

Y

T

R

S

S

G

S

F

F

T

T

S

P

M

M

Y

Y

T

F

A

A

V

L

T

G

S

E

H

Y

R

R

Q

Q

A

K

C

C

K

D

M

M

K

K

L

L

V

I

C

C

A

V

G

G

K

K

E

E

E

E

K

K

V

I

V

V

G

G

I

L

H

H

G

G

I

I

G

G

F

I

G

G

M

V

D

D

E

E

I

M

L

L

Q

Q

G

G

F

F

G

A

V

V

A

A

M

I

K

K

M

M

G

G

A

A

T

T

K

K

A

A

D

D

F

F

D

D

A

N

V

T

V

V

A
|
A

I

I

H
|
H

P

P

T

T

G

G

A

S

E
|
E

E

E

F

F

V

V

T

T

M

M

R

R

active site: I39 (≠ V38), C43 (= C42), C48 (= C47), K51 (= K50), Y178 (= Y177), E182 (= E181), A438 (= A438), H440 (= H440), E445 (= E445)
binding flavin-adenine dinucleotide: I11 (≠ L10), G14 (= G13), S15 (= S14), G16 (= G15), E35 (= E34), G36 (≠ A35), G41 (= G40), T42 (= T41), C43 (= C42), G47 (= G46), C48 (= C47), K51 (= K50), Y115 (= Y114), A116 (≠ G115), T140 (= T139), G141 (= G140), Y178 (= Y177), I179 (= I178), G303 (= G302), D304 (= D303), D310 (≠ E310), L311 (= L311), T312 (= T312)

6du7A Glutathione reductase from streptococcus pneumoniae (see paper)
56% identity, 96% coverage: 19:451/451 of query aligns to 18:448/448 of 6du7A

query
sites
6du7A

S

T

A

M

N

N

R

R

A

A

A

G

M

E

R

H

G

G

A

A

K

Q

V

A

L

A

L

V

I
|
I

E
|
E

A
x
E

K

K

H

K

V

L

G

G

G
|
G

T
|
T

C
|
C

V

V

N

N

V

V

G
|
G

C
|
C

V

V

P

P

K
|
K

K

K

V

I

M

M

W

W

Y

Y

G

G

A

A

H

Q

I

I

A

A

E

E

A

T

M

F

N

H

L

Q

Y

F

A

G

K

E

D

D

Y

Y

G

G

F

F

D

K

V

T

S

T

V

D

N

L

K

N

F

F

D

D

W

F

N

A

T

T

L

L

V

R

N

R

S

N

R

R

E

E

A

S

Y

Y

I

I

G

D

R

R

I

A

H

R

E

S

A

S

Y

Y

G

D

R

G

G

S

F

F

T

K

N

R

N

N

K

G

V

V

T

D

L

L

L

I

N

E

G

G

Y
x
H

G
x
A

R

E

F

F

V

V

N

D

G

S

N

H

T

T

I

V

E

S

V

V

N

N

G

G