SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

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Comparing 74 a.a. (KRNRIPLGCT...) to proteins with known functional sites using BLASTp with E ≤ 0.001.

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Found 7 hits to proteins with known functional sites (download)

P0CS82 Heme-responsive zinc finger transcription factor HAP1; CYP1 activatory protein; Heme activator protein 1 from Saccharomyces cerevisiae (Baker's yeast) (see 2 papers)
99% identity, 100% coverage: 1:74/74 of query aligns to 56:129/1483 of P0CS82

query
sites
P0CS82

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S63 (≠ G8) mutation to R: In CYP1-18; activates the expression of CYP3 (Iso-2) while reducing that of CYC1 (Iso-1).

2hapC Structure of a hap1-18/DNA complex reveals that protein/DNA interactions can have direct allosteric effects on transcriptional activation (see paper)
99% identity, 100% coverage: 1:74/74 of query aligns to 2:75/76 of 2hapC

query
sites
2hapC

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binding zinc ion: C10 (= C9), C10 (= C9), C13 (= C12), C20 (= C19), C27 (= C26), C27 (= C26), C30 (= C29), C39 (= C38)
binding : K2 (= K1), R3 (= R2), R5 (= R4), R9 (≠ G8), R16 (= R15), K17 (= K16), K17 (= K16)

Sites not aligning to the query:

binding : 1

Q1MTM9 Uncharacterized transcriptional regulatory protein C1327.01c from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
55% identity, 45% coverage: 2:34/74 of query aligns to 65:97/977 of Q1MTM9

query
sites
Q1MTM9

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Sites not aligning to the query:

220 modified: Phosphoserine

Q59QC7 Sterol uptake control protein 2 from Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) (see paper)
35% identity, 78% coverage: 8:65/74 of query aligns to 53:110/712 of Q59QC7

query
sites
Q59QC7

G

G

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Sites not aligning to the query:

478 W→C: Leads to increased ERG11 expression, increased cellular ergosterol, and decreased susceptibility to fluconazole.
642 Y→F: Leads to increased ERG11 expression, increased cellular ergosterol, and decreased susceptibility to fluconazole.
643 mutation A->T,V: Leads to increased ERG11 expression, increased cellular ergosterol, and decreased susceptibility to fluconazole.
646 A→V: Leads to increased ERG11 expression, increased cellular ergosterol, and decreased susceptibility to fluconazole.
648 mutation G->D,S: Leads to increased ERG11 expression, increased cellular ergosterol, and decreased susceptibility to fluconazole.

P39001 Transcriptional regulatory protein UME6; Negative transcriptional regulator of IME2; Regulator of inducer of meiosis protein 16; Unscheduled meiotic gene expression protein 6 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
37% identity, 81% coverage: 8:67/74 of query aligns to 770:829/836 of P39001

query
sites
P39001

G

G

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Sites not aligning to the query:

99 mutation T->N,A: Impairs meiotic genes expression and sporulation, reduces interactions with IME1 and RIM11, and reduces phosphorylation.
99:109 TPVHTPSGSPS→APVHAPAGAPA: Impairs meiotic genes expression, sporulation and interactions with IME1 and RIM11, and abolishes phosphorylation.
103 T→A: Impairs meiotic genes expression and sporulation, reduces interaction with IME, and reduces phosphorylation.
107 S→A: Impairs meiotic genes expression and sporulation, reduces interaction with IME, and reduces phosphorylation.
523 A→S: Impairs SIN3-binding and gene repression activity.
524 A→T: Impairs gene repression activity.
525 mutation Missing: Impairs gene repression activity.
526 V→Q: Impairs gene repression activity.
527 L→P: Impairs SIN3-binding and gene repression activity.
528 S→P: Impairs SIN3-binding and gene repression activity.
530 mutation M->T,V: Impairs SIN3-binding and gene repression activity.
635 K→E: Impairs gene repression activity.

I1RC73 Zn(2)-C6 fungal-type transcription factor from Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) (Wheat head blight fungus) (Fusarium graminearum) (see paper)
52% identity, 36% coverage: 8:34/74 of query aligns to 13:39/492 of I1RC73

query
sites
I1RC73

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Sites not aligning to the query:

82 modified: Phosphothreonine; T→A: Impairs phosphorylation by HOG1, blocks induction of CYP51A expression and exhibits hypersensitivity to tebuconazole; when associated with A-92, A-102, A-217 and A-305.; T→D: Mimics phosphorylation by HOG1; when associated with D-92, D-102, D-217 and D-305.
92 modified: Phosphoserine; S→A: Impairs phosphorylation by HOG1, blocks induction of CYP51A expression and exhibits hypersensitivity to tebuconazole; when associated with A-82, A-102, A-217 and A-305.; S→D: Mimics phosphorylation by HOG1; when associated with D-82, D-102, D-217 and D-305.
102 modified: Phosphoserine; S→A: Impairs phosphorylation by HOG1, blocks induction of CYP51A expression and exhibits hypersensitivity to tebuconazole; when associated with A-82, A-92, A-217 and A-305.; S→D: Mimics phosphorylation by HOG1; when associated with D-82, D-92, D-217 and D-305.
217 modified: Phosphothreonine; T→A: Impairs phosphorylation by HOG1, blocks induction of CYP51A expression and exhibits hypersensitivity to tebuconazole; when associated with A-82, A-92, A-102 and A-305.; T→D: Mimics phosphorylation by HOG1; when associated with D-82, D-92, D-102 and D-305.
305 modified: Phosphoserine; S→A: Impairs phosphorylation by HOG1, blocks induction of CYP51A expression and exhibits hypersensitivity to tebuconazole; when associated with A-82, A-92, A-102 and A-217.; S→D: Mimics phosphorylation by HOG1; when associated with D-82, D-92, D-102 and D-217.

Q5A4G2 Multidrug resistance regulator 1 from Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) (see 5 papers)
43% identity, 54% coverage: 1:40/74 of query aligns to 23:61/1108 of Q5A4G2

query
sites
Q5A4G2

K

K

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Y

Sites not aligning to the query:

683 P→S: Leads to constitutive MDR1 overexpression and multidrug resistance.
997 G→V: Leads to constitutive MDR1 overexpression and multidrug resistance.

Query Sequence

>74 a.a. (KRNRIPLGCT...)
KRNRIPLGCTICRKRKVKCDKLRPHCQQCTKTGVAHLCHYMEQTWAEEAEKELLKDNELK
KLRERVKSLEKTLS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory