SitesBLAST

12 H→L: Increases the stability of phosphorylated form.
56 D→A: Prevents both swarming motility and pellicle formation.; D→N: Lack of phosphorylation. Decreases degradative enzyme production, but allows full expression of late competence genes.
98 T→I: Increases phosphorylation.
131 V→L: Increases phosphorylation.

4wu4B Crystal structure of e. Faecalis DNA binding domain liard191n complexed with 22bp DNA (see paper)
47% identity, 80% coverage: 12:70/74 of query aligns to 7:65/68 of 4wu4B

query
sites
4wu4B

L

L

T
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T

K

N

R
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R

E

E

R

H

E

E

V

I

F

L

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M

L

L

L

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A

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D

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x
N

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S

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Y

L

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H

G

G

binding : T8 (= T13), R10 (= R15), N24 (≠ T29), I33 (= I38), T34 (≠ S39), K36 (= K41), K36 (= K41), T37 (= T42), K39 (≠ R44), H41 (= H46), S43 (= S48), N53 (≠ G58), R54 (= R59)

5hevF Crystal structure of the beryllofluoride-activated liar from enterococcus faecium (see paper)
46% identity, 80% coverage: 12:70/74 of query aligns to 149:207/210 of 5hevF

query
sites
5hevF

L

L

T

T

K

N

R

R

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E

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H

G

G

Sites not aligning to the query:

binding beryllium trifluoride ion: 54, 55, 56, 82, 104
binding magnesium ion: 9, 54, 56

P94439 Transcriptional regulatory protein LnrK from Bacillus subtilis (strain 168) (see paper)
47% identity, 80% coverage: 12:70/74 of query aligns to 158:216/220 of P94439

query
sites
P94439

L

L

T

T

K

E

R

R

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Y

G

G

Sites not aligning to the query:

54 D→A: Mutant is sensitive to linearmycins.

P9WGM5 Probable transcriptional regulatory protein NarL from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
34% identity, 92% coverage: 1:68/74 of query aligns to 144:211/216 of P9WGM5

query
sites
P9WGM5

M

I

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R

Sites not aligning to the query:

61 modified: 4-aspartylphosphate; D→N: Complete loss of phosphorylation.

4ldzA Crystal structure of the full-length response regulator desr in the active state (see paper)
56% identity, 70% coverage: 12:63/74 of query aligns to 140:191/196 of 4ldzA

query
sites
4ldzA

L

L

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Sites not aligning to the query:

binding beryllium trifluoride ion: 56, 57, 58, 82, 83, 84, 104
binding magnesium ion: 11, 56, 58

P0AGA6 Transcriptional regulatory protein UhpA from Escherichia coli (strain K12) (see paper)
59% identity, 62% coverage: 12:57/74 of query aligns to 138:183/196 of P0AGA6

query
sites
P0AGA6

L

L

T

T

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K

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S

Sites not aligning to the query:

54 modified: 4-aspartylphosphate; D→N: Lack of phosphorylation.

P9WMF9 DNA-binding transcriptional activator DevR/DosR from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 4 papers)
45% identity, 72% coverage: 12:64/74 of query aligns to 150:202/217 of P9WMF9

query
sites
P9WMF9

L

L

T

T

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D

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V

T198 (≠ S60) modified: Phosphothreonine; by PknH; mutation to A: Decreases phosphorylation by PknH.

Sites not aligning to the query:

8 D→N: No phosphorylation by DevS (DosS).
9 D→N: No phosphorylation by DevS (DosS).
54 D→E: Able to bind DNA, no longer induces hypoxic response.; D→V: No phosphorylation by DevS (DosS) nor by DosT.
82 T→A: No longer induces expression of DevR regulon under hypoxic conditions, phosphorylated slowly and incompletely by DevS, decreased cooperative binding of target DNA.
104 K→E: No phosphorylation by DevS (DosS).
205 modified: Phosphothreonine; by PknH; T→A: No phosphorylation by PknH.

1zlkA Crystal structure of the mycobacterium tuberculosis hypoxic response regulator dosr c-terminal domain-DNA complex (see paper)
46% identity, 74% coverage: 10:64/74 of query aligns to 2:58/65 of 1zlkA

query
sites
1zlkA

P

P

L

L

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S

-

G

L

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Q

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binding : Q9 (≠ R15), T22 (= T28), L32 (≠ I38), K35 (= K41), T36 (= T42), K38 (≠ R44), N39 (= N45), Y40 (≠ H46), S42 (= S48), R53 (= R59)

3kloA Vibrio cholerae vpst bound to c-di-gmp (see paper)
43% identity, 72% coverage: 12:64/74 of query aligns to 157:209/217 of 3kloA

query
sites
3kloA

L

L

T

T

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Sites not aligning to the query:

binding 9,9'-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one): 129, 131, 132

7ve5A C-terminal domain of vrar (see paper)
44% identity, 73% coverage: 11:64/74 of query aligns to 5:58/66 of 7ve5A

query
sites
7ve5A

L

M

L

L

T

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binding : S22 (≠ T28), N23 (≠ T29), K38 (≠ R44), S42 (= S48), D52 (≠ G58), R53 (= R59)

Q7A2Q1 Response regulator protein VraR from Staphylococcus aureus (strain Mu50 / ATCC 700699) (see paper)
44% identity, 73% coverage: 11:64/74 of query aligns to 147:200/209 of Q7A2Q1

query
sites
Q7A2Q1

L

M

L

L

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I

Sites not aligning to the query:

55 modified: 4-aspartylphosphate; D→A: Complete loss of phosphorylation.

4if4A Crystal structure of the magnesium and beryllofluoride-activated vrar from staphylococcus aureus (see paper)
44% identity, 73% coverage: 11:64/74 of query aligns to 146:199/208 of 4if4A

query
sites
4if4A

L

M

L

L

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I

Sites not aligning to the query:

binding beryllium trifluoride ion: 54, 55, 56, 82, 83, 104
binding magnesium ion: 9, 54, 56

Q2FX09 Response regulator protein VraR from Staphylococcus aureus (strain NCTC 8325 / PS 47) (see paper)
44% identity, 73% coverage: 11:64/74 of query aligns to 147:200/209 of Q2FX09

query
sites
Q2FX09

L

M

L

L

T

T

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Sites not aligning to the query:

13 M→A: Loss of dimerization and binding to target promoter.

3qp6A Crystal structure of cvir (chromobacterium violaceum 12472) bound to c6-hsl (see paper)
42% identity, 72% coverage: 12:64/74 of query aligns to 192:244/258 of 3qp6A

query
sites
3qp6A

L

L

T

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V

V

Sites not aligning to the query:

binding N-[(3S)-2-oxotetrahydrofuran-3-yl]hexanamide: 74, 78, 91, 105, 109, 120, 147, 149

3qp5A Crystal structure of cvir bound to antagonist chlorolactone (cl) (see paper)
42% identity, 72% coverage: 12:64/74 of query aligns to 190:242/251 of 3qp5A

query
sites
3qp5A

L

L

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K

Q

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N

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V

Sites not aligning to the query:

binding 4-(4-chlorophenoxy)-N-[(3S)-2-oxotetrahydrofuran-3-yl]butanamide: 49, 76, 80, 103, 127, 147

4yn8A Crystal structure of response regulator chra in heme-sensing two component system (see paper)
41% identity, 76% coverage: 8:63/74 of query aligns to 135:192/201 of 4yn8A

query
sites
4yn8A

S

S

K

K

P

P

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R

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Q

L

A

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A

A

A

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I

Sites not aligning to the query:

binding magnesium ion: 10, 12, 24, 26, 29, 52

7r3hA Pross optimitzed variant of rhlr (75 mutations) in complex with native autoinducer c4-hsl (see paper)
35% identity, 95% coverage: 1:70/74 of query aligns to 164:234/236 of 7r3hA

query
sites
7r3hA

M

L

K

N

E

H

K

P

E

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F

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Q

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K

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P

P

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C

L

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N

N

F

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H

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N

A

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K

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Q

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A

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Y

L

A

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A

M

L

G

G

Sites not aligning to the query:

binding N-[(3S)-2-oxotetrahydrofuran-3-yl]butanamide: 59, 63, 78, 79, 91, 106, 130

7r3gB Pross optimitzed variant of rhlr (75 mutations) in complex with the synthetic antagonist mbtl (see paper)
35% identity, 95% coverage: 1:70/74 of query aligns to 164:234/236 of 7r3gB

query
sites
7r3gB

M

L

K

N

E

H

K

P

E

S

F

L

Q

Q

S

P

K

Q

P

P

L

I

-

C

L

L

T

S

K

K

R

R

E

E

R

R

E

E

V

I

F

L

E

R

L

W

L

T

V

A

Q

D

D

G

K

K

T

T

T

S

K

A

E

E

I

I

A

A

S

K

E

I

L

L

F

G

I

I

S

S

E

E

K

S

T

T

V

V

R

N

N

F

H

H

I

L

S

K

N

N

A

I

M

Q

Q

K

K

K

L

F

G

N

V

A

K

P

G

N

R

K

S

T

Q

Q

A

A

V

A

E

Y

L

A

R

A

M

L

G

G

Sites not aligning to the query:

binding 4-(3-bromophenoxy)-N-[(3S)-2-oxothiolan-3-yl]butanamide: 39, 41, 55, 56, 59, 63, 64, 67, 76, 78, 79, 91, 96, 130

Q7AKE4 Response regulator RamR; Transcriptional activator RamR from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) (see 2 papers)
40% identity, 70% coverage: 12:63/74 of query aligns to 142:193/202 of Q7AKE4

query
sites
Q7AKE4

L

L

T

T

K

T

R

R

E

E

R

L

E

G

V

V

F

L

E

T

L

L

L

A

V

S

Q

Q

D

G

K

A

T

P

T

I

K

A

E

E

I

I

A

A

S

A

E

R

L

L

F

H

I

L

S

S

E

R

K

G

T

T

V

V

R

R

N

N

H

Y

I

M

S

A

N

T

A

A

M

V

Q

R

K

K

L

V

G

G

V

A

K

R

G

N

R

R

S

V

Q

D

A

A

V

I

Sites not aligning to the query:

12 D→A: Restores aerial hyphae formation in ramR deletion mutants, activates RamC expression.
56 D→A: Does not restore aerial hyphae formation in ramR deletion mutants. Does not activate RamC expression, does not bind ramC promoter, does not form homodimers.; D→E: Restores aerial hyphae formation in ramR deletion mutants, does not bind ramC promoter.; D→N: Does not restore aerial hyphae formation in ramR deletion mutants, does not bind ramC promoter, forms homodimers.
84 mutation T->A,I: Restores aerial hyphae formation in ramR deletion mutants, activates RamC expression.
102 mutation L->A,W: Does not restore aerial hyphae formation in ramR deletion mutants, does not activate RamC expression. Has lost cooperative binding to the ramC promoter, binds only the high affinity site, forms homodimers.; L→Y: Restores aerial hyphae formation in ramR deletion mutants, activates RamC expression.
105 K→A: Does not restore aerial hyphae formation in ramR deletion mutants, does not activate RamC expression, does not bind ramC promoter, does not form homodimers.

Query Sequence

>74 a.a. (MKEKEFQSKP...)
MKEKEFQSKPLLTKREREVFELLVQDKTTKEIASELFISEKTVRNHISNAMQKLGVKGRS
QAVVELLRMGELEL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory