SitesBLAST
Comparing 8499267 FitnessBrowser__Miya:8499267 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
Q51742 Ornithine carbamoyltransferase, anabolic; OTCase; EC 2.1.3.3 from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) (see 3 papers)
46% identity, 97% coverage: 3:294/300 of query aligns to 8:306/315 of Q51742
- W22 (= W17) mutation to A: Decreased heat stability.
- E26 (≠ Q21) mutation to Q: Increased dissociation of dodecamers into trimers.
- M30 (≠ E25) mutation to A: Increased dissociation of dodecamers into trimers.
- W34 (≠ T29) mutation to A: Increased dissociation of dodecamers into trimers.
- Y228 (≠ N216) mutation to C: Becomes active at low temperatures; when associated with G-278.
- A241 (≠ Q229) mutation to D: Becomes active at low temperatures; when associated with G-278.
- E278 (= E266) mutation to G: Becomes active at low temperatures; when associated with C-228 or D-241.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
8qeuA Crystal structure of ornithine transcarbamylase from arabidopsis thaliana (atotc) in complex with ornithine (see paper)
47% identity, 97% coverage: 3:294/300 of query aligns to 2:298/304 of 8qeuA
8qevA Crystal structure of ornithine transcarbamylase from arabidopsis thaliana (atotc) in complex with carbamoyl phosphate (see paper)
45% identity, 97% coverage: 3:294/300 of query aligns to 2:291/297 of 8qevA
Q81M99 Ornithine carbamoyltransferase; OTCase; EC 2.1.3.3 from Bacillus anthracis
46% identity, 88% coverage: 35:298/300 of query aligns to 43:309/316 of Q81M99
- STRT 57:60 (= STRT 49:52) binding carbamoyl phosphate
- Q84 (= Q76) binding carbamoyl phosphate
- R108 (= R100) binding carbamoyl phosphate
- HPCQ 135:138 (= HPCQ 127:130) binding carbamoyl phosphate
- N166 (= N158) binding L-ornithine
- D230 (= D218) binding L-ornithine
- SM 234:235 (= SM 222:223) binding L-ornithine
- CL 269:270 (= CL 258:259) binding carbamoyl phosphate
- R297 (= R286) binding carbamoyl phosphate
4nf2A Crystal structure of anabolic ornithine carbamoyltransferase from bacillus anthracis in complex with carbamoyl phosphate and l- norvaline
46% identity, 88% coverage: 35:298/300 of query aligns to 39:305/307 of 4nf2A
- active site: R55 (= R51), T56 (= T52), R83 (= R79), R104 (= R100), H131 (= H127), Q134 (= Q130), D226 (= D218), C265 (= C258), R293 (= R286)
- binding phosphoric acid mono(formamide)ester: S53 (= S49), T54 (= T50), R55 (= R51), T56 (= T52), R104 (= R100), H131 (= H127), Q134 (= Q130), C265 (= C258), L266 (= L259), R293 (= R286)
- binding norvaline: L126 (= L122), N162 (= N158), D226 (= D218), S230 (= S222), M231 (= M223)
P9WIT9 Ornithine carbamoyltransferase; OTCase; EC 2.1.3.3 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
44% identity, 99% coverage: 1:296/300 of query aligns to 1:302/307 of P9WIT9
- STRT 50:53 (= STRT 49:52) binding carbamoyl phosphate
- Q77 (= Q76) binding carbamoyl phosphate
- R101 (= R100) binding carbamoyl phosphate
- HPCQ 128:131 (= HPCQ 127:130) binding carbamoyl phosphate
- N160 (= N158) binding L-ornithine
- D224 (= D218) binding L-ornithine
- SM 228:229 (= SM 222:223) binding L-ornithine
- CL 264:265 (= CL 258:259) binding carbamoyl phosphate
- R292 (= R286) binding carbamoyl phosphate
2i6uA Crystal structure of ornithine carbamoyltransferase complexed with carbamoyl phosphate and l-norvaline from mycobacterium tuberculosis (rv1656) at 2.2 a (see paper)
44% identity, 98% coverage: 3:296/300 of query aligns to 3:302/307 of 2i6uA
- active site: R52 (= R51), T53 (= T52), R80 (= R79), R101 (= R100), H128 (= H127), Q131 (= Q130), D224 (= D218), C264 (= C258), R292 (= R286)
- binding phosphoric acid mono(formamide)ester: S50 (= S49), T51 (= T50), R52 (= R51), T53 (= T52), R101 (= R100), C264 (= C258), L265 (= L259), R292 (= R286)
- binding norvaline: L123 (= L122), N160 (= N158), D224 (= D218), S228 (= S222), M229 (= M223)
7nouA Crystal structure of mycobacterium tuberculosis argf in complex with (3,5-dichlorophenyl)boronic acid.
44% identity, 98% coverage: 3:296/300 of query aligns to 4:303/308 of 7nouA
- active site: R102 (= R100), H129 (= H127), Q132 (= Q130), D225 (= D218), C265 (= C258), R293 (= R286)
- binding [3,5-bis(chloranyl)phenyl]-oxidanyl-oxidanylidene-boron: I46 (= I44), T52 (= T50), R53 (= R51), R53 (= R51), F56 (≠ V54), F56 (≠ V54), L79 (= L77), D82 (≠ S80), E83 (= E81), V91 (= V89), Y95 (= Y93), L266 (= L259), R293 (= R286)
7nosA Crystal structure of mycobacterium tuberculosis argf in complex with 4-bromo-6-(trifluoromethyl)-1h-benzo[d]imidazole.
44% identity, 98% coverage: 3:296/300 of query aligns to 4:303/308 of 7nosA
7norA Crystal structure of mycobacterium tuberculosis argf in complex with 2-fluoro-4-hydroxybenzonitrile.
44% identity, 98% coverage: 3:296/300 of query aligns to 4:303/308 of 7norA
7nnyA Crystal structure of mycobacterium tuberculosis argf in complex with naphthalen-1-ol.
44% identity, 98% coverage: 3:296/300 of query aligns to 4:303/308 of 7nnyA
- active site: R102 (= R100), H129 (= H127), Q132 (= Q130), D225 (= D218), C265 (= C258), R293 (= R286)
- binding 1-naphthol: T52 (= T50), R53 (= R51), F56 (≠ V54), E83 (= E81), V91 (= V89), Y95 (= Y93)
7nnwA Crystal structure of mycobacterium tuberculosis argf in complex with methyl 4-hydroxy-3-iodobenzoate.
44% identity, 98% coverage: 3:296/300 of query aligns to 4:303/308 of 7nnwA
- active site: R102 (= R100), H129 (= H127), Q132 (= Q130), D225 (= D218), C265 (= C258), R293 (= R286)
- binding methyl 3-iodanyl-4-oxidanyl-benzoate: I46 (= I44), T52 (= T50), R53 (= R51), F56 (≠ V54), L79 (= L77), L92 (= L90), Y95 (= Y93)
7nnvA Crystal structure of mycobacterium tuberculosis argf in complex with carbamoyl phosphate.
44% identity, 98% coverage: 3:296/300 of query aligns to 4:303/308 of 7nnvA
- active site: R102 (= R100), H129 (= H127), Q132 (= Q130), D225 (= D218), C265 (= C258), R293 (= R286)
- binding phosphoric acid mono(formamide)ester: S51 (= S49), T52 (= T50), R53 (= R51), T54 (= T52), R102 (= R100), H129 (= H127), C265 (= C258), L266 (= L259), R293 (= R286)
7np0A Crystal structure of mycobacterium tuberculosis argf in complex with (4-nitrophenyl)boronic acid.
44% identity, 98% coverage: 3:296/300 of query aligns to 4:300/305 of 7np0A
7novA Crystal structure of mycobacterium tuberculosis argf in complex with (4-methyl-3-nitrophenyl)boronic acid.
43% identity, 98% coverage: 3:296/300 of query aligns to 4:297/302 of 7novA
- active site: R96 (= R100), H123 (= H127), Q126 (= Q130), D219 (= D218), C259 (= C258), R287 (= R286)
- binding (4-methyl-3-nitro-phenyl)-oxidanyl-oxidanylidene-boron: R53 (= R51), F56 (≠ V54), E77 (= E81), V85 (= V89), Y89 (= Y93), L260 (= L259), A284 (= A283), R287 (= R286)
7nnzB Crystal structure of mycobacterium tuberculosis argf in complex with 5-methyl-4-phenylthiazol-2-amine.
43% identity, 98% coverage: 3:296/300 of query aligns to 3:292/297 of 7nnzB
P00480 Ornithine transcarbamylase, mitochondrial; OTCase; Ornithine carbamoyltransferase, mitochondrial; EC 2.1.3.3 from Homo sapiens (Human) (see 31 papers)
46% identity, 84% coverage: 35:286/300 of query aligns to 76:330/354 of P00480
- K88 (= K47) modified: N6-acetyllysine; alternate; to N: in OTCD; late onset; dbSNP:rs72554339
- STRT 90:93 (= STRT 49:52) binding carbamoyl phosphate
- G100 (≠ A59) to D: in OTCD; late onset; dbSNP:rs72554349
- F101 (≠ V60) to L: in dbSNP:rs1133135
- L111 (≠ M70) to P: in dbSNP:rs1800324
- T125 (≠ R84) to M: in OTCD; neonatal; dbSNP:rs72554356
- D126 (= D85) to G: in OTCD; early onset; loss of ornithine carbamoyltransferase activity; 0.9% of wild-type activity; dbSNP:rs72554358
- R129 (= R88) to H: in OTCD; early onset; decreased ornithine carbamoyltransferase activity; 2.1% of wild-type activity; dbSNP:rs66656800
- A140 (≠ V99) to P: in OTCD; late onset; dbSNP:rs72556260
- R141 (= R100) binding carbamoyl phosphate; to Q: in OTCD; most common variant; loss of ornithine carbamoyltransferase activity; activity is 100-fold lower; dbSNP:rs68026851
- H168 (= H127) binding carbamoyl phosphate
- Q171 (= Q130) binding carbamoyl phosphate
- I172 (≠ V131) to M: in OTCD; early onset; loss of ornithine carbamoyltransferase activity; dbSNP:rs72556280
- Y176 (≠ V135) to C: in OTCD; late onset; dbSNP:rs72556283
- TL 178:179 (≠ TM 137:138) natural variant: Missing (in OTCD; neonatal)
- Y183 (≠ T142) to D: in OTCD; late onset; dbSNP:rs72556292
- G188 (≠ T147) to R: in OTCD; neonatal; dbSNP:rs72556294
- G195 (= G154) to R: in OTCD; loss of ornithine carbamoyltransferase activity; dbSNP:rs67294955
- D196 (= D155) to V: in OTCD; neonatal; decreased ornithine carbamoyltransferase activity; 3.7% activity; dbSNP:rs72556300
- L201 (≠ A160) to P: in OTCD; neonatal; dbSNP:rs72558407
- S207 (≠ A166) to R: in OTCD; neonatal; dbSNP:rs72558415
- A209 (≠ V168) to V: in OTCD; neonatal; dbSNP:rs72558417
- M213 (≠ F172) to K: in OTCD; late onset
- H214 (≠ E173) to Y: in OTCD; neonatal; dbSNP:rs72558420
- P220 (= P179) to A: in OTCD; late onset; dbSNP:rs72558425
- P225 (= P184) to T: in OTCD; late onset; dbSNP:rs72558428
- L244 (≠ I199) to Q: in OTCD; late onset; dbSNP:rs72558436
- T262 (= T217) to K: in OTCD; mild; dbSNP:rs67333670
- T264 (≠ V219) to A: in OTCD; late onset; decreased ornithine carbamoyltransferase activity; 8.9% activity; dbSNP:rs72558444; to I: in OTCD; late onset; dbSNP:rs67156896
- W265 (= W220) to L: in OTCD; mild; dbSNP:rs72558446
- G269 (= G224) to E: in OTCD; neonatal; dbSNP:rs72558450
- Q270 (= Q225) to R: in dbSNP:rs1800328
- E272 (= E227) natural variant: Missing (in OTCD; late onset; dbSNP:rs72558452)
- R277 (= R232) to Q: in OTCD; late onset; dbSNP:rs66724222; to W: in OTCD; late onset; dbSNP:rs72558454
- H302 (= H257) to L: in OTCD; female; late onset; dbSNP:rs67993095; to Y: in OTCD; neonatal; dbSNP:rs72558463
- C303 (= C258) to R: in OTCD; neonatal; dbSNP:rs67468335
- CL 303:304 (= CL 258:259) binding carbamoyl phosphate
- E309 (= E265) natural variant: Missing (in OTCD; late onset)
- R330 (= R286) binding carbamoyl phosphate
Sites not aligning to the query:
- 1:32 modified: transit peptide, Mitochondrion
- 15 R→G: Loss of cleavage of the transit peptide and loss of localization to mitochondrial matrix; when associated with G-23 and G-26.
- 23 R→G: Loss of cleavage of the transit peptide and loss of localization to mitochondrial matrix; when associated with G-15 and G-26.
- 26 R→G: Loss of cleavage of the transit peptide and loss of localization to mitochondrial matrix; when associated with G-15 and G-23.
- 39 G → C: in OTCD; late onset; dbSNP:rs72554306
- 40 R → H: in OTCD; late onset; dbSNP:rs72554308
- 43 L → F: in dbSNP:rs72554309
- 46 K → R: in dbSNP:rs1800321
- 55 Y → D: in OTCD; late onset; dbSNP:rs72554319
- 63 L → P: in OTCD; late onset; dbSNP:rs72554324
- 333 natural variant: T -> A
- 340 S → P: in OTCD; late onset; dbSNP:rs72558489
- 343 T → K: in OTCD; late onset; dbSNP:rs72558491
1othA Crystal structure of human ornithine transcarbamoylase complexed with n-phosphonacetyl-l-ornithine (see paper)
45% identity, 84% coverage: 35:286/300 of query aligns to 43:297/321 of 1othA
- active site: R59 (= R51), T60 (= T52), V87 (≠ R79), R108 (= R100), H135 (= H127), Q138 (= Q130), D230 (= D218), C270 (= C258), R297 (= R286)
- binding n-(phosphonoacetyl)-l-ornithine: S57 (= S49), T58 (= T50), R59 (= R51), T60 (= T52), R108 (= R100), L130 (= L122), H135 (= H127), N166 (= N158), D230 (= D218), S234 (= S222), M235 (= M223), C270 (= C258), L271 (= L259), R297 (= R286)
1c9yA Human ornithine transcarbamylase: crystallographic insights into substrate recognition and catalytic mechanism (see paper)
45% identity, 84% coverage: 35:286/300 of query aligns to 43:297/321 of 1c9yA
- active site: R59 (= R51), T60 (= T52), V87 (≠ R79), R108 (= R100), H135 (= H127), Q138 (= Q130), D230 (= D218), C270 (= C258), R297 (= R286)
- binding phosphoric acid mono(formamide)ester: S57 (= S49), T58 (= T50), R59 (= R51), T60 (= T52), R108 (= R100), C270 (= C258), L271 (= L259), R297 (= R286)
- binding norvaline: L130 (= L122), N166 (= N158), D230 (= D218), S234 (= S222), M235 (= M223)
P04391 Ornithine carbamoyltransferase subunit I; OTCase-1; EC 2.1.3.3 from Escherichia coli (strain K12) (see 7 papers)
41% identity, 99% coverage: 3:300/300 of query aligns to 7:334/334 of P04391
- S56 (= S49) mutation to H: Much less active than the wild-type.
- STRT 56:59 (= STRT 49:52) binding carbamoyl phosphate
- R58 (= R51) mutation to G: The mutant is drastically inefficient in catalysis, but affects only moderately the binding of carbamoyl phosphate.
- Q83 (= Q76) binding carbamoyl phosphate
- K87 (≠ S80) mutation to Q: Much less active than the wild-type.
- R107 (= R100) binding carbamoyl phosphate
- HPTQ 134:137 (≠ HPCQ 127:130) binding carbamoyl phosphate
- N168 (= N158) binding L-ornithine
- D232 (= D218) binding L-ornithine
- SM 236:237 (= SM 222:223) binding L-ornithine
- C274 (= C258) binding Zn(2+); mutation to A: Zinc ion is no longer a tight-binding inhibitor and does not promote isomerization.
- CL 274:275 (= CL 258:259) binding carbamoyl phosphate
- R320 (= R286) binding carbamoyl phosphate; mutation to A: Much less active than the wild-type.
- A326 (= A292) mutation to G: Activity greater than the wild-type and Km for ornithwinas increases about twofold.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
Query Sequence
>8499267 FitnessBrowser__Miya:8499267
MPRHFTRIRDLGYEGAWKLLQRAKEMKDTAHRGKIMEGKTAILIFEKASTRTRVSFEMAV
RHLGGSTIFMTPAESQLGRSEPLRDTARVLSRYADCMVVRTFGQSKITELAEYGSIPVIN
ALTDEGHPCQVMGDVLTMYERTPDLSTVRVAWVGDGNNMANSWIEAAVYFPFELFMAFPE
GYEPDRDLLGYALNAGAKIFLTHDPRMAVEGAHYVNTDVWASMGQEEEQKKREAAFKGYC
IDTEMMALAAPDAKFMHCLPAHRGEEVTDEVMESPASIIFDQAENRLHIQKAILEWAFSE
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory