SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 8499376 FitnessBrowser__Miya:8499376 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P09114 Acetolactate synthase 2, chloroplastic; ALS II; Acetohydroxy-acid synthase II; Acetolactate synthase II; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see paper)
47% identity, 99% coverage: 4:560/562 of query aligns to 92:655/664 of P09114

query
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P09114

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P191 (= P102) mutation to A: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with L-568.
W568 (= W480) mutation to L: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with A-191.

P09342 Acetolactate synthase 1, chloroplastic; ALS I; Acetohydroxy-acid synthase I; Acetolactate synthase I; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see 2 papers)
47% identity, 99% coverage: 4:560/562 of query aligns to 95:658/667 of P09342

query
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P09342

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C161 (= C69) modified: Disulfide link with 307
P194 (= P102) mutation to Q: In C3; highly resistant to sulfonylurea herbicides.
C307 (≠ V213) modified: Disulfide link with 161

P17597 Acetolactate synthase, chloroplastic; AtALS; Acetohydroxy-acid synthase; Protein CHLORSULFURON RESISTANT 1; EC 2.2.1.6 from Arabidopsis thaliana (Mouse-ear cress) (see 8 papers)
46% identity, 99% coverage: 4:560/562 of query aligns to 98:661/670 of P17597

query
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P17597

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V

P

P

K
|
K

D

D

V

I

M

Q

Q

Q

A

Q

K

L

T

A

E

I

F

P

V

N

W

W

P

E

E

Q

D

A

V

M

K

R

L

L

R

P

S

G

Y

Y

N

M

P

S

T

R

Y

M

-

P

-

K

R

P

P

P

N

E

L

D

N

S

Q

H

L

L

R

E

R

Q

A

I

V

V

E

R

A

L

L

I

I

S

E

E

A

S

K

K

R

K

P

P

L

V

F

L

F

Y

A

V

G

G

G
|
G

G

G

V

C

I

L

M

-

S

-

D

N

A

S

S

S

E

D

E

E

L

L

G

G

W

R

L

F

A

V

R

E

T

L

L

T

S

G

I

I

P

P

V

V

T

A

S

S

T
|
T

L
|
L

M

M

G

G

L

L

G

G

A

S

F

Y

P

P

G
x
C

D

D

P

E

L

L

W

S

L

L

G

H

M

M

I
x
L

G
|
G

M
|
M

H
|
H

G

G

T

T

Y

V

A

Y

G

A

N

N

K

Y

A

A

V

V

N

E

N

H

A

S

D

D

C

L

I

L

V

L

A

A

V

F

G
|
G

A
x
V

R
|
R

F
|
F

D
|
D

R
|
R

V

V

T

T

G

G

R

K

L

L

S

E

A

A

F

F

A

A

S

S

G

R

A

A

R

K

I

I

I

V

H

H

I

I

D
|
D

I
|
I

D

D

P

S

T

A

S

E

I

I

R

G

K

K

N

N

V

K

Q

T

V

P

D

H

I

V

P

S

V

V

V

C

G

G

D
|
D

C
x
V

R

K

K

L

A

A

L

L

A

Q

G

G

I

M

R

N

E

K

I

V

A

L

T

E

T

N

R

R

L

A

A

E

E

E

-

L

-

K

K

L

D

D

W

F

A

G

T

V

A

-

H

-

E

-

P

-

W

W

L

R

T

N

Q

E

V

L

S

N

A

V

W

Q

R

K

T

Q

E

K

Q

F

P

P

L

L

T

S

Y

F

V

K

K

T

N

F

G

G

-

E

S

A

I

I

K

P

P

P

Q

Q

Q

Y

V

A

V

I

E

K

T

V

I

L

F

D

S

E

I

L

T

T

R

D

G

G

D

K

A

A

I

I

V

I

T

S

T

T

E

G

V

V

G

G

Q
|
Q

N
x
H

Q

Q

M

M

W

W

T

A

A

A

Q

Q

F

F

F

Y

T

N

F

Y

R

K

K

K

P

P

R

R

T

Q

L

W

L

L

T

S

S

S

G
|
G

L

L

G
|
G

T
x
A

M
|
M

G

G

Y

F

G

G

F

L

P

P

A

A

I

I

G

G

A

A

Q

S

F

V

A

A

F

N

P

P

D

D

K

A

L

I

V

V

I

V

D

D

M

I

A

D

G

G

D
|
D

G
|
G

S
|
S

I

F

Q

I

M

M

N

N

I

V

Q

Q

E

E

L

L

A

A

T

T

A

I

V

R

C

V

N

E

K

N

L

L

P

P

I

V

K

K

I

V

V

L

I

L

L

L

N

N

N
|
N

G
x
Q

Y
x
H

L
|
L

G
|
G

M
|
M

V

V

R

M

Q

Q

W
|
W

Q

E

E

D

L

R

F

F

Y

Y

Q

K

R

A

N

N

Y

R

C

A

S

H

T

T

C

F

M

L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

E

E

A

I

Q

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

A

A

Y

C

G

G

A

I

E

P

G

A

Y

A

R

R

I

V

T

T

E

K

A

K

G

A

E

D

L

L

E

R

S

E

T

A

L

I

R

Q

A

T

A

M

F

L

A

D

S

T

P

P

H

G

T

P

A

Y

V

L

I

L

D

D

V

V

R

I

V

C

E

P

R

H

E

Q

E

E

N

H

V

V

Y

L

P

P

M

M

V

I

P

P

A
x
S

G

G

A

G

A

T

L

F

D

N

E

D

M

V

L

I

A122 (= A28) mutation to V: Reduced catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
M124 (≠ I30) mutation to E: Reduced catalytic activity. Resistant to imidazolinone herbicides and reduced sensitivity to sulfonylurea herbicides.; mutation to I: No effect on catalytic activity. Increased resistance to imidazolinone herbicides.
E144 (= E49) binding
S186 (= S91) binding
P197 (= P102) mutation to S: In csr1-1/GH50; resistant to sulfonylurea but not to imidazolinone herbicides.
R199 (≠ P104) mutation R->A,E: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
Q207 (= Q112) binding
K220 (= K125) binding
R246 (= R151) binding ; binding
K256 (= K161) binding
G308 (= G211) binding
TL 331:332 (= TL 236:237) binding
C340 (≠ G245) modified: Cysteine sulfinic acid (-SO2H)
LGMH 349:352 (≠ IGMH 254:257) binding
GVRFD 371:375 (≠ GARFD 276:280) binding
DR 376:377 (= DR 281:282) binding
DI 395:396 (= DI 300:301) binding
DV 414:415 (≠ DC 319:320) binding
QH 487:488 (≠ QN 393:394) binding
GG 508:509 (= GG 414:415) binding
GAM 511:513 (≠ GTM 417:419) binding
D538 (= D444) binding
DGS 538:540 (= DGS 444:446) binding
N565 (= N471) binding
NQHLGM 565:570 (≠ NGYLGM 471:476) binding
H567 (≠ Y473) binding
W574 (= W480) binding ; mutation to L: Increased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.; mutation to S: Slightly decreased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.
S653 (≠ A552) binding ; mutation to A: No effect on catalytic activity or sensitivity to herbicides.; mutation to F: No effect on catalytic activity. Resistant to imidazolinone herbicides and also slightly sulfonylurea-resistant.; mutation to N: In csr1-2/GH90; no effect on catalytic activity. Resistant to imidazolinone but not to sulfonylurea herbicides.; mutation to T: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.

8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
46% identity, 99% coverage: 4:560/562 of query aligns to 13:576/582 of 8et4A

query
sites
8et4A

N

K

G

G

A

A

R

D

I

I

L

L

L

V

E

E

C

A

L

L

K

E

R

R

E

Q

G

G

V

V

D

E

V

T

L

V

F

F

G

A

Y

Y

P

P

G

G

A

A

V

S

I

M

D

E

I

I

Y

H

D

Q

E

A

L

L

P

T

R

R

H

S

D

S

-

S

I

I

R

R

H

N

I

V

L

L

V

P

R

R

H

H

E

E

Q

Q

A

G

V

V

H

F

A

A

D

E

G

G

F

Y

A

A

R

R

A

S

S

S

G

G

K

K

V

P

G

G

V

I

C

C

L

I

V

A

T

T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

T

L

V

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

A

L

D

D

S

S

I

V

P

P

M

L

V

V

V

A

L

I

T

T

G

G

Q

Q

V

V

P

P

T

R

P

R

L

M

I

I

G

G

N

T

D

D

A

A

F

F

Q

Q

E

E

V

T

D

P

I

I

V

V

G

E

I

V

T

T

R

R

P

S

C

I

T

T

K

K

H

H

N

N

Y

Y

L

L

V

V

K

M

D

D

V

V

R

E

D

D

L

I

A

P

R

R

V

I

L

I

R

E

Q

E

A

A

F

F

Y

F

L

L

A

A

R

T

S

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

V

V

D

D

L

V

P

P

K

K

D

D

V

I

M

Q

Q

Q

A

Q

K

L

T

A

E

I

F

P

V

N

W

W

P

E

E

Q

D

A

V

M

K

R

L

L

R

P

S

G

Y

Y

N

M

P

S

T

R

Y

M

-

P

-

K

R

P

P

P

N

E

L

D

N

S

Q

H

L

L

R

E

R

Q

A

I

V

V

E

R

A

L

L

I

I

S

E

E

A

S

K

K

R

K

P

P

L

V

F

L

F

Y

A

V

G
|
G

V

C

I

L

M

-

S

-

D

N

A

S

S

S

E

D

E

E

L

L

G

G

W

R

L

F

A

V

R

E

T

L

L

T

S

G

I

I

P

P

V

V

T

A

S

S

T
|
T

L
|
L

M
|
M

G

G

L

L

G

G

A

S

F

Y

P

P

G

C

D

D

P

E

L

L

W

S

L

L

G

H

M
|
M

I
x
L

G
|
G

M
|
M

H
|
H

G

G

T

T

Y

V

A

Y

G

A

N

N

K

Y

A

A

V

V

N

E

N

H

A

S

D

D

C

L

I

L

V

L

A

A

V

F

G
|
G

A
x
V

R
|
R

F

F

D
|
D

D

D

R
|
R

V
|
V

T

T

G

G

R

K

L

L

S

E

A

A

F

F

A

A

S

S

G

R

A

A

R

K

I

I

I

V

H

H

I

I

D
|
D

I
|
I

D
|
D

P

S

T

A

S
x
E

I

I

R

G

K

K

N

N

V

K

Q

T

V

P

D

H

I

V

P

S

V

V

V

C

G
|
G

D
|
D

C
x
V

R

K

K

L

A

A

L

L

A

Q

G

G

I

M

R

N

E

K

I

V

A

L

T

E

T

N

R

R

L

A

A

E

E

E

-

L

-

K

K

L

D

D

W

F

A

G

T

V

A

-

H

-

E

-

P

-

W

W

L

R

T

N

Q

E

V

L

S

N

A

V

W

Q

R

K

T

Q

E

K

Q

F

P

P

L

L

T

S

Y
x
F

V

K

K

T

N

F

G

G

-

E

S

A

I

I

K

P

P

P

Q

Q

Q
x
Y

V

A

V

I

E

K

T

V

I

L

F

D

S

E

I

L

T

T

R

D

G

G

D

K

A

A

I

I

V

I

T

S

T

T

E

G

V
|
V

G
|
G

Q
|
Q

N
x
H

Q
|
Q

M
|
M

W

W

T

A

A

A

Q

Q

F

F

F

Y

T

N

F

Y

R

K

K

K

P

P

R

R

T

Q

L

W

L

L

T

S

S

S

G
|
G

L

L

G
|
G

T
x
A

M
|
M

G

G

Y

F

G

G

F

L

P

P

A

A

I

I

G

G

A

A

Q

S

F

V

A

A

F

N

P

P

D

D

K

A

L

I

V

V

I

V

D

D

M

I

A

D

G
|
G

D
|
D

G
|
G

S
|
S

I

F

Q

I

M
|
M

N

N

I

V

Q
|
Q

E

E

L

L

A

A

T

T

A

I

V

R

C

V

N

E

K

N

L

L

P

P

I

V

K

K

I

V

V

L

I

L

L

L

N

N

N
|
N

G

Q

Y
x
H

L
|
L

G
|
G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

E

D

L

R

F

F

Y

Y

Q

K

R

A

N

N

Y

R

C

A

S

H

T

T

C

F

M

L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

E

E

A

I

Q

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

A

A

Y

C

G

G

A

I

E

P

G

A

Y

A

R

R

I

V

T

T

E

K

A
x
K

G

A

E

D

L

L

E

R

S

E

T

A

L

I

R

Q

A

T

A

M

F

L

A

D

S

T

P

P

H

G

T

P

A

Y

V

L

I

L

D

D

V

V

R

I

V

C

E

P

R

H

E

Q

E

E

N

H

V

V

Y

L

P

P

M

M

V

I

P

P

A
x
S

G

G

A

G

A

T

L

F

D

N

E

D

M

V

L

I

binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V391), G401 (= G392), Q402 (= Q393), H403 (≠ N394), G426 (= G417), A427 (≠ T418), M428 (= M419), G452 (= G443), D453 (= D444), G454 (= G445), S455 (= S446), M458 (= M449), N480 (= N471), H482 (≠ Y473), L483 (= L474), G484 (= G475), M485 (= M476), V486 (= V477)
binding flavin-adenine dinucleotide: R161 (= R151), G222 (= G210), G223 (= G211), G224 (= G212), T246 (= T236), L247 (= L237), M248 (= M238), M263 (= M253), L264 (≠ I254), G265 (= G255), M266 (= M256), H267 (= H257), G286 (= G276), V287 (≠ A277), R288 (= R278), D290 (= D280), R292 (= R282), V293 (= V283), D310 (= D300), I311 (= I301), D312 (= D302), E315 (≠ S305), G328 (= G318), D329 (= D319), V330 (≠ C320), Q404 (= Q395), M405 (= M396), G423 (= G414), G424 (= G415)
binding magnesium ion: F370 (≠ Y362), Y381 (≠ Q372), D453 (= D444), M458 (= M449), Q461 (= Q452), N480 (= N471), H482 (≠ Y473), K533 (≠ A517)
binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: M266 (= M256), H267 (= H257), R292 (= R282), M485 (= M476), V486 (= V477), W489 (= W480), S568 (≠ A552)

5wj1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a triazolopyrimidine herbicide, penoxsulam (see paper)
46% identity, 99% coverage: 4:560/562 of query aligns to 13:576/582 of 5wj1A

query
sites
5wj1A

N

K

G

G

A

A

R

D

I

I

L

L

L

V

E

E

C

A

L

L

K

E

R

R

E

Q

G

G

V

V

D

E

V

T

L

V

F

F

G

A

Y
|
Y

P

P

G
|
G

A
|
A

V
x
S

I

M

D

E

I

I

Y

H

D

Q

E

A

L

L

P

T

R

R

H

S

D

S

-

S

I

I

R

R

H

N

I

V

L

L

V

P

R

R

H

H

E
|
E

Q

Q

A

G

V

V

H

F

A

A

D

E

G

G

F

Y

A

A

R

R

A

S

S

S

G

G

K

K

V

P

G

G

V

I

C

C

L

I

V

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

T

L

V

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

A

L

D

D

S

S

I

V

P

P

M

L

V

V

V

A

L

I

T

T

G

G

Q

Q

V

V

P

P

T

R

P

R

L

M

I

I

G

G

N

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

V

T

D

P

I

I

V

V

G

E

I

V

T

T

R

R

P

S

C

I

T

T

K

K

H

H

N

N

Y

Y

L

L

V

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K

M

D

D

V

V

R

E

D

D

L

I

A

P

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R

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I

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I

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E

Q

E

A

A

F

F

Y

F

L

L

A

A

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T

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S

G

G

R
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R

P

P

G

G

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P

V

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L

L

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D

D

L

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P

K
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K

D

D

V

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Q

Q

A

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I

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W

W

P

E

E

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A

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L

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Y

Y

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-

P

-

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A

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G

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-

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N

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M

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M

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x
L

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G

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M

H
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H

G

G

T

T

Y

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A

Y

G

A

N

N

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Y

A

A

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V

N

E

N

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A

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A

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G

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x
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D

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I
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I

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D

I
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I

D
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D

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E

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N

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x
V

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G

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N

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K

I

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A

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T

N

R

R

L

A

A

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E

E

-

L

-

K

K

L

D

D

W

F

A

G

T

V

A

-

H

-

E

-

P

-

W

W

L

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N

Q

E

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L

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N

A

V

W

Q

R

K

T

Q

E

K

Q

F

P

P

L

L

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Y

F

V

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T

N

F

G

G

-

E

S

A

I

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Y

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A

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I

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I

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D

K

A

A

I

I

V

I

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T

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E

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V

G
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G

Q
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Q

N
x
H

Q
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Q

M
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M

W

W

T

A

A

A

Q

Q

F

F

F

Y

T

N

F

Y

R

K

K

K

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P

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R

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Q

L

W

L

L

T

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G
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G

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L

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G

T
x
A

M
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M

G

G

Y

F

G

G

F

L

P

P

A

A

I

I

G

G

A

A

Q

S

F

V

A

A

F

N

P

P

D

D

K

A

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I

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V

I

V

D

D

M

I

A

D

G
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G

D
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D

G
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G

S
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S

I

F

Q

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M
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M

N

N

I

V

Q

Q

E

E

L

L

A

A

T

T

A

I

V

R

C

V

N

E

K

N

L

L

P

P

I

V

K

K

I

V

V

L

I

L

L

L

N

N

N
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N

G

Q

Y
x
H

L
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L

G
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G

M
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M

V
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V

R

M

Q

Q

W
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W

Q

E

E

D

L

R

F

F

Y

Y

Q

K

R

A

N

N

Y

R

C

A

S

H

T

T

C

F

M

L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

E

E

A

I

Q

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

A

A

Y

C

G

G

A

I

E

P

G

A

Y

A

R

R

I

V

T

T

E

K

A

K

G

A

E

D

L

L

E

R

S

E

T

A

L

I

R

Q

A

T

A

M

F

L

A

D

S

T

P

P

H

G

T

P

A

Y

V

L

I

L

D

D

V

V

R

I

V

C

E

P

R
x
H

E

Q

E

E

N

H

V

V

Y

L

P

P

M

M

V

I

P

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x
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A

G

A

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L

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E

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I

active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ V29), E59 (= E49), T82 (= T72), F121 (= F111), Q122 (= Q112), E123 (= E113), K171 (= K161), M266 (= M256), V293 (= V283), V400 (= V391), G426 (= G417), M428 (= M419), D453 (= D444), N480 (= N471), H482 (≠ Y473), L483 (= L474), M485 (= M476), V486 (= V477), W489 (= W480), H558 (≠ R542)
binding flavin-adenine dinucleotide: R161 (= R151), G222 (= G210), G223 (= G211), G224 (= G212), T246 (= T236), L247 (= L237), M248 (= M238), M263 (= M253), L264 (≠ I254), G265 (= G255), M266 (= M256), H267 (= H257), G286 (= G276), V287 (≠ A277), R288 (= R278), D290 (= D280), R292 (= R282), V293 (= V283), I309 (= I299), D310 (= D300), I311 (= I301), D312 (= D302), E315 (≠ S305), G328 (= G318), D329 (= D319), V330 (≠ C320), Q404 (= Q395), M405 (= M396), G423 (= G414), G424 (= G415), M485 (= M476)
binding magnesium ion: D453 (= D444), N480 (= N471), H482 (≠ Y473)
binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M266 (= M256), D291 (= D281), R292 (= R282), M485 (= M476), V486 (= V477), W489 (= W480), S568 (≠ A552), G569 (= G553)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V391), G401 (= G392), Q402 (= Q393), H403 (≠ N394), G426 (= G417), A427 (≠ T418), M428 (= M419), G452 (= G443), D453 (= D444), G454 (= G445), S455 (= S446), M458 (= M449), N480 (= N471), H482 (≠ Y473), L483 (= L474), G484 (= G475), M485 (= M476), V486 (= V477)

5k6tA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, propoxycarbazone-sodium (see paper)
46% identity, 99% coverage: 4:560/562 of query aligns to 13:576/582 of 5k6tA

query
sites
5k6tA

N

K

G

G

A

A

R

D

I

I

L

L

L

V

E

E

C

A

L

L

K

E

R

R

E

Q

G

G

V

V

D

E

V

T

L

V

F

F

G

A

Y
|
Y

P

P

G
|
G

A
|
A

V
x
S

I

M

D

E

I

I

Y

H

D

Q

E

A

L

L

P

T

R

R

H

S

D

S

-

S

I

I

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R

H

N

I

V

L

L

V

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R

R

H

H

E
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E

Q

Q

A

G

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V

H

F

A

A

D

E

G

G

F

Y

A

A

R

R

A

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G

G

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K

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P

G

G

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I

C

C

L

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T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

T

L

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V

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G

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D

A

A

Y

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A

L

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M

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V

A

L

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V

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G

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E

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R

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K

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L

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Y

Y

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M

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-

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A

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L

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-

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N

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-

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x
H

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M
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M

W

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A

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Q

F

F

F

Y

T

N

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Y

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L

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G

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P

A

A

I

I

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G

A

A

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F

V

A

A

F

N

P

P

D

D

K

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I

V

D

D

M

I

A

D

G
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G

D
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G
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S
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S

I

F

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M
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M

N

N

I

V

Q

Q

E

E

L

L

A

A

T

T

A

I

V

R

C

V

N

E

K

N

L

L

P

P

I

V

K

K

I

V

V

L

I

L

L

L

N

N

N
|
N

G

Q

Y
x
H

L
|
L

G
|
G

M
|
M

V
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V

R

M

Q

Q

W
|
W

Q

E

E

D

L

R

F

F

Y

Y

Q

K

R

A

N

N

Y

R

C

A

S

H

T

T

C

F

M

L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

E

E

A

I

Q

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

A

A

Y

C

G

G

A

I

E

P

G

A

Y

A

R

R

I

V

T

T

E

K

A

K

G

A

E

D

L

L

E

R

S

E

T

A

L

I

R

Q

A

T

A

M

F

L

A

D

S

T

P

P

H

G

T

P

A

Y

V

L

I

L

D

D

V

V

R

I

V

C

E

P

R
x
H

E

Q

E

E

N

H

V

V

Y

L

P

P

M

M

V

I

P

P

A
x
S

G

G

A

G

A

T

L

F

D

N

E

D

M

V

L

I

active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ V29), E59 (= E49), T82 (= T72), F121 (= F111), Q122 (= Q112), E123 (= E113), K171 (= K161), M266 (= M256), V293 (= V283), V400 (= V391), G426 (= G417), M428 (= M419), D453 (= D444), N480 (= N471), H482 (≠ Y473), L483 (= L474), M485 (= M476), V486 (= V477), W489 (= W480), H558 (≠ R542)
binding methyl 2-[(4-methyl-5-oxidanylidene-3-propoxy-1,2,4-triazol-1-yl)carbonylsulfamoyl]benzoate: H267 (= H257), D291 (= D281), R292 (= R282), M485 (= M476), W489 (= W480), S568 (≠ A552)
binding flavin-adenine dinucleotide: R161 (= R151), G222 (= G210), G223 (= G211), G224 (= G212), T246 (= T236), L247 (= L237), M248 (= M238), M263 (= M253), L264 (≠ I254), G265 (= G255), M266 (= M256), H267 (= H257), G286 (= G276), V287 (≠ A277), R288 (= R278), D290 (= D280), R292 (= R282), V293 (= V283), I309 (= I299), D310 (= D300), I311 (= I301), D312 (= D302), E315 (≠ S305), G328 (= G318), D329 (= D319), V330 (≠ C320), Q404 (= Q395), M405 (= M396), G423 (= G414), G424 (= G415)
binding magnesium ion: D453 (= D444), N480 (= N471), H482 (≠ Y473)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (= V391), G401 (= G392), Q402 (= Q393), H403 (≠ N394), G426 (= G417), M428 (= M419), G452 (= G443), D453 (= D444), G454 (= G445), S455 (= S446), M458 (= M449), N480 (= N471), H482 (≠ Y473), L483 (= L474), G484 (= G475), M485 (= M476), V486 (= V477)

5k6rA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, thiencarbazone-methyl (see paper)
46% identity, 99% coverage: 4:560/562 of query aligns to 13:576/582 of 5k6rA

query
sites
5k6rA

N

K

G

G

A

A

R

D

I

I

L

L

L

V

E

E

C

A

L

L

K

E

R

R

E

Q

G

G

V

V

D

E

V

T

L

V

F

F

G

A

Y
|
Y

P

P

G
|
G

A
|
A

V
x
S

I

M

D

E

I

I

Y

H

D

Q

E

A

L

L

P

T

R

R

H

S

D

S

-

S

I

I

R

R

H

N

I

V

L

L

V

P

R

R

H

H

E
|
E

Q

Q

A

G

V

V

H

F

A

A

D

E

G

G

F

Y

A

A

R

R

A

S

S

S

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active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ V29), E59 (= E49), T82 (= T72), F121 (= F111), Q122 (= Q112), E123 (= E113), K171 (= K161), M266 (= M256), V293 (= V283), V400 (= V391), G426 (= G417), M428 (= M419), D453 (= D444), N480 (= N471), H482 (≠ Y473), L483 (= L474), M485 (= M476), V486 (= V477), W489 (= W480), H558 (≠ R542)
binding methyl 4-[(3-methoxy-4-methyl-5-oxidanylidene-1,2,4-triazol-1-yl)carbonylsulfamoyl]-5-methyl-thiophene-3-carboxylate: D291 (= D281), R292 (= R282), W489 (= W480), S568 (≠ A552)
binding flavin-adenine dinucleotide: R161 (= R151), G222 (= G210), G223 (= G211), G224 (= G212), T246 (= T236), L247 (= L237), M248 (= M238), M263 (= M253), L264 (≠ I254), G265 (= G255), M266 (= M256), H267 (= H257), G286 (= G276), V287 (≠ A277), R288 (= R278), D290 (= D280), R292 (= R282), V293 (= V283), I309 (= I299), D310 (= D300), I311 (= I301), D312 (= D302), E315 (≠ S305), G328 (= G318), D329 (= D319), V330 (≠ C320), Q404 (= Q395), M405 (= M396), G423 (= G414), G424 (= G415)
binding magnesium ion: D453 (= D444), N480 (= N471), H482 (≠ Y473)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (= V391), G401 (= G392), Q402 (= Q393), H403 (≠ N394), G426 (= G417), M428 (= M419), G452 (= G443), D453 (= D444), G454 (= G445), S455 (= S446), M458 (= M449), N480 (= N471), H482 (≠ Y473), L483 (= L474), G484 (= G475), M485 (= M476), V486 (= V477)

1z8nA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with an imidazolinone herbicide, imazaquin (see paper)
46% identity, 99% coverage: 4:560/562 of query aligns to 13:576/582 of 1z8nA

query
sites
1z8nA

N

K

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A

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L