SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 8499412 FitnessBrowser__Miya:8499412 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 12 hits to proteins with known functional sites (download)

Q96XT4 2-oxoacid:ferredoxin oxidoreductase 2, subunit beta; OFOR2; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
40% identity, 93% coverage: 14:275/283 of query aligns to 11:290/304 of Q96XT4

query
sites
Q96XT4

W

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C12 (= C15) binding
C15 (= C18) binding
IGCS 44:47 (≠ IGQA 47:50) binding
C46 (≠ Q49) binding
D90 (= D91) binding
GD 91:92 (≠ GC 92:93) binding
N118 (= N119) binding
V120 (≠ I121) binding
GL 122:123 (= GL 123:124) binding
C197 (= C198) binding

5b46B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - ligand free form (see paper)
40% identity, 93% coverage: 14:275/283 of query aligns to 8:287/301 of 5b46B

query
sites
5b46B

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W

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binding magnesium ion: D87 (= D91), N115 (= N119), V117 (≠ I121)
binding iron/sulfur cluster: W8 (= W14), C9 (= C15), C12 (= C18), C43 (≠ Q49), C194 (= C198), T196 (≠ S200), Y197 (≠ F201)
binding thiamine diphosphate: I41 (= I47), G42 (= G48), C43 (≠ Q49), S44 (≠ A50), H62 (= H66), G86 (= G90), G88 (= G92), D89 (≠ C93), N115 (= N119), V117 (≠ I121), Y118 (= Y122), G119 (= G123), L120 (= L124), T121 (= T125)

P72579 2-oxoacid:ferredoxin oxidoreductase subunit beta; OFOR; EC 1.2.7.11 from Sulfolobus sp. (see paper)
44% identity, 83% coverage: 14:249/283 of query aligns to 11:259/305 of P72579

query
sites
P72579

W

W

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C

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P

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K49 (= K52) mutation to I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to R: Increase the oxidoreductase activity with pyruvate.; mutation to V: Slight decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.
L123 (= L124) mutation L->A,I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to N: Strong decrease of the oxidoreductase activity with pyruvate and 2-oxobutyrate. However, this mutant shows almost the same activity with 2-oxoglutarate as the wild-type.

Q96Y68 2-oxoacid:ferredoxin oxidoreductase 1, subunit beta; OFOR1; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 2 papers)
44% identity, 83% coverage: 14:249/283 of query aligns to 11:259/305 of Q96Y68

query
sites
Q96Y68

W

W

C
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C

P

P

G

G

C
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C

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G

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N

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C12 (= C15) binding
C15 (= C18) binding
C46 (≠ Q49) binding
K49 (= K52) mutation to I: Loss of oxidoreductase activity toward 2-oxoglutarate but retains its activity toward pyruvate.
D90 (= D91) binding
GD 91:92 (≠ GC 92:93) binding
N118 (= N119) binding
V120 (≠ I121) binding
GL 122:123 (= GL 123:124) binding
K125 (= K126) Plays an important role in the binding of CoA; mutation to A: Shows a strong decrease of affinity for CoA and a poor inactivation by 4-fluoro-7-nitrobenzofurazan (NBD-F).
K173 (≠ P174) mutation to A: Same oxidoreductase activity as the wild-type.
C197 (= C198) binding

6n2oD 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
35% identity, 95% coverage: 12:280/283 of query aligns to 22:290/291 of 6n2oD

query
sites
6n2oD

T

T

A

T

W
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W

C
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C

P

P

G

G

C
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C

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G

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G

G

G

D
|
D

G
|
G

C
x
D

N

G

Y

F

G

S

E

I

G

G

N

G

H

H

F

M

L

P

A

H

A

M

I

A

R

R

R

K

N

N

V

V

D

N

M

M

T

T

L

Y

L

V

V

L

H

M

D

D

N
|
N

Q

G

I
|
I

Y

Y

G
|
G

L
|
L

T
|
T

K
|
K

G

G

Q

Q

A

Y

S

S

P

P

T

T

T

S

P

R

E

P

G

E

Q

M

I

T

T

A

K

Y

S

T

Q

T

P

P

D

Y

G

G

V

G

R

P

N

E

A

N

P

P

F

M

N

N

P

P

I

L

A

L

V

Y

A

M

V

L

A

T

M

Y

K

G

C

A

S

T

F

Y

V

V

A

A

R

Q

S

A

F

F

S

A

G

G

N

K

T

P

P

K

H

D

L

C

V

A

K

E

M

L

I

I

Q

K

L

G

A

A

I

M

Q

E

H

H

K

E

G

G

F

F

A

A

L

Y

V

V

D

N

L

I

F

F

S

S

P

Q

C
|
C

V

P

S
x
T

F
|
F

N

N

K

K

V

I

N

D

T

T

F

V

G

D

W

F

Y

Y

K

R

Q

D

R

L

C

V

Q

E

E

P

L

I

P

P

E

E

D

D

Y

H

D

D

P

T

T

S

D

D

W

L

A

G

A

A

M

M

Q

E

V

L

A

A

Q

R

E

R

F

P

G

G

E

G

R

K

I

A

P

P

I

T

G

G

V

L

I

L

Y

Y

R

K

N

T

D

S

R

A

P

P

S

T

L

L

D

D

A

Q

S

N

L

L

P

A

V

K

L

I

Q

R

K

E

G

R

P

L

L

G

G

G

R

H

Q

V

P

G

V

Y

D

D

R

K

D

N

G

K

L

I

R

I

A

A

L

L

M

A

Q

K

binding magnesium ion: D101 (= D91), N129 (= N119), I131 (= I121)
binding succinyl-coenzyme a: I57 (= I47), R62 (≠ K52), L134 (= L124), K136 (= K126)
binding iron/sulfur cluster: W24 (= W14), C25 (= C15), C28 (= C18), C59 (≠ Q49), C208 (= C198), T210 (≠ S200), F211 (= F201)
binding thiamine diphosphate: I57 (= I47), G58 (= G48), C59 (≠ Q49), S60 (≠ A50), H76 (= H66), G102 (= G92), D103 (≠ C93), N129 (= N119), I131 (= I121), G133 (= G123), L134 (= L124), T135 (= T125)

6n2oB 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
35% identity, 95% coverage: 12:280/283 of query aligns to 22:290/291 of 6n2oB

query
sites
6n2oB

T

T

A

T

W
|
W

C
|
C

P

P

G

G

C
|
C

G

G

N

H

H

F

D

G

V

I

M

L

K

N

A

G

V

V

R

Y

Q

R

A

A

L

M

A

A

G

E

L

L

D

G

L

I

A

D

P

S

N

T

R

K

V

F

A

A

H

A

V

I

S

S

G

G

I
|
I

G
|
G

Q
x
C

A
x
S

A

S

K
x
R

A

M

P

P

H

Y

Y

F

I

V

D

D

L

S

N

Y

G

K

F

M

N

H

G

T

L

L

H
|
H

G

G

R

R

G

A

L

G

P

A

P

V

A

A

Q

T

A

G

I

T

K

Q

L

V

C

A

N

R

P

P

E

D

L

L

T

C

V

V

I

V

A

V

Q

A

S

G

G

G

D
|
D

G
|
G

C
x
D

N

G

Y

F

G

S

E

I

G

G

N

G

H

H

F

M

L

P

A

H

A

M

I

A

R

R

R

K

N

N

V

V

D

N

M

M

T

T

L

Y

L

V

V

L

H

M

D

D

N
|
N

Q

G

I
|
I

Y
|
Y

G
|
G

L
|
L

T
|
T

K
|
K

G

G

Q

Q

A

Y

S

S

P

P

T

T

T

S

P

R

E

P

G

E

Q

M

I

T

T

A

K
x
Y

S

T

Q

T

P

P

D

Y

G

G

V

G

R

P

N

E

A

N

P

P

F

M

N

N

P

P

I

L

A

L

V

Y

A

M

V

L

A

T

M

Y

K

G

C

A

S

T

F

Y

V

V

A

A

R

Q

S

A

F

F

S

A

G

G

N

K

T

P

P

K

H

D

L

C

V

A

K

E

M

L

I

I

Q

K

L

G

A

A

I

M

Q

E

H

H

K

E

G

G

F

F

A

A

L

Y

V

V

D

N

L

I

F

F

S

S

P

Q

C
|
C

V

P

S
x
T

F
|
F

N

N

K

K

V

I

N

D

T

T

F

V

G

D

W

F

Y

Y

K

R

Q

D

R

L

C

V

Q

E

E

P

L

I

P

P

E

E

D

D

Y

H

D

D

P

T

T

S

D

D

W

L

A

G

A

A

M

M

Q

E

V

L

A

A

Q

R

E

R

F

P

G

G

E

G

R

K

I

A

P

P

I

T

G

G

V

L

I

L

Y

Y

R

K

N

T

D

S

R

A

P

P

S

T

L

L

D

D

A

Q

S

N

L

L

P

A

V

K

L

I

Q

R

K

E

G

R

P

L

L

G

G

G

R

H

Q

V

P

G

V

Y

D

D

R

K

D

N

G

K

L

I

R

I

A

A

L

L

M

A

Q

K

binding 2-oxoglutaric acid: R62 (≠ K52), L134 (= L124)
binding coenzyme a: K136 (= K126), Y150 (≠ K140)
binding magnesium ion: D101 (= D91), N129 (= N119), I131 (= I121)
binding iron/sulfur cluster: W24 (= W14), C25 (= C15), C28 (= C18), C59 (≠ Q49), C208 (= C198), T210 (≠ S200), F211 (= F201)
binding thiamine diphosphate: I57 (= I47), G58 (= G48), C59 (≠ Q49), S60 (≠ A50), H76 (= H66), G102 (= G92), D103 (≠ C93), N129 (= N119), I131 (= I121), Y132 (= Y122), G133 (= G123), L134 (= L124), T135 (= T125)

6n2nB Crystal structure of 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus (see paper)
35% identity, 95% coverage: 12:280/283 of query aligns to 22:290/291 of 6n2nB

query
sites
6n2nB

T

T

A

T

W
|
W

C
|
C

P

P

G

G

C
|
C

G

G

N
x
H

H

F

D

G

V

I

M

L

K

N

A

G

V

V

R

Y

Q

R

A

A

L

M

A

A

G

E

L

L

D

G

L

I

A

D

P

S

N

T

R

K

V

F

A

A

H

A

V

I

S

S

G

G

I
|
I

G
|
G

Q
x
C

A
x
S

A

S

K

R

A

M

P

P

H

Y

Y

F

I

V

D

D

L

S

N

Y

G

K

F

M

N

H

G

T

L

L

H
|
H

G

G

R

R

G

A

L

G

P

A

P

V

A

A

Q

T

A

G

I

T

K

Q

L

V

C

A

N

R

P

P

E

D

L

L

T

C

V

V

I

V

A

V

Q

A

S

G

G
|
G

D
|
D

G
|
G

C
x
D

N

G

Y

F

G

S

E

I

G

G

N

G

H

H

F

M

L

P

A

H

A

M

I

A

R

R

R

K

N

N

V

V

D

N

M

M

T

T

L

Y

L

V

V

L

H

M

D

D

N
|
N

Q

G

I
|
I

Y
|
Y

G
|
G

L
|
L

T
|
T

K

K

G

G

Q

Q

A

Y

S

S

P

P

T

T

T

S

P

R

E

P

G

E

Q

M

I

T

T

A

K

Y

S

T

Q

T

P

P

D

Y

G

G

V

G

R

P

N

E

A

N

P

P

F

M

N

N

P

P

I

L

A

L

V

Y

A

M

V

L

A

T

M

Y

K

G

C

A

S

T

F

Y

V

V

A

A

R

Q

S

A

F

F

S

A

G

G

N

K

T

P

P

K

H

D

L

C

V

A

K

E

M

L

I

I

Q

K

L

G

A

A

I

M

Q

E

H

H

K

E

G

G

F

F

A

A

L

Y

V

V

D

N

L

I

F

F

S

S

P

Q

C
|
C

V

P

S
x
T

F
|
F

N

N

K

K

V

I

N

D

T

T

F

V

G

D

W

F

Y

Y

K

R

Q

D

R

L

C

V

Q

E

E

P

L

I

P

P

E

E

D

D

Y

H

D

D

P

T

T

S

D

D

W

L

A

G

A

A

M

M

Q

E

V

L

A

A

Q

R

E

R

F

P

G

G

E

G

R

K

I

A

P

P

I

T

G

G

V

L

I

L

Y

Y

R

K

N

T

D

S

R

A

P

P

S

T

L

L

D

D

A

Q

S

N

L

L

P

A

V

K

L

I

Q

R

K

E

G

R

P

L

L

G

G

G

R

H

Q

V

P

G

V

Y

D

D

R

K

D

N

G

K

L

I

R

I

A

A

L

L

M

A

Q

K

binding magnesium ion: D101 (= D91), N129 (= N119), I131 (= I121)
binding iron/sulfur cluster: W24 (= W14), C25 (= C15), C28 (= C18), H30 (≠ N20), C59 (≠ Q49), C208 (= C198), T210 (≠ S200), F211 (= F201)
binding thiamine diphosphate: I57 (= I47), G58 (= G48), C59 (≠ Q49), S60 (≠ A50), H76 (= H66), G100 (= G90), D101 (= D91), G102 (= G92), D103 (≠ C93), N129 (= N119), I131 (= I121), Y132 (= Y122), G133 (= G123), L134 (= L124), T135 (= T125)

5b48B 2-oxoacid:ferredoxin oxidoreductase 1 from sulfolobus tokodai (see paper)
40% identity, 92% coverage: 14:274/283 of query aligns to 7:271/286 of 5b48B

query
sites
5b48B

W

W

C
|
C

P

P

G

G

C
|
C

G

G

N

N

H

F

D

G

V

I

M

L

K

N

A

A

V

E

R

Q

Q

Q

A

A

L

I

A

V

G

E

L

L

D

G

L

V

A

D

P

T

N

K

R

N

V

V

A

V

H

V

V

V

S

S

G

G

I
|
I

G
|
G

Q
x
C

A
x
S

A

G

K

K

A

I

P

P

H

H

Y

F

I

T

D

P

L

I

N

S

G

G

F

V

N

H

G

T

L

L

H
|
H

G

G

R

R

G

A

L

I

P

A

P

F

A

A

Q

T

A

G

I

I

K

K

L

L

C

S

N

N

P

P

E

D

L

L

T

V

V

V

I

I

A

V

Q

N

S

G

G

G

D
|
D

G
|
G

C
x
D

N

L

Y

L

G

G

E

I

G

G

G

A

N

G

H

H

F

F

L

V

A

A

I

G

R

R

N

N

V

V

D

D

M

M

T

V

L

V

L

I

V

L

H

H

D

D

N
|
N

Q

G

I
x
V

Y
|
Y

G
|
G

L
|
L

T

T

K

K

G

G

Q

Q

A

A

S

S

P

P

T

T

T

L

P

K

E

R

G

G

Q

E

I

N

T

I

K

-

S

-

Q

-

P

-

D

-

G

-

V

-

R

-

N

N

A

D

P

A

F

V

N

N

P

P

I

I

A

A

V

L

A

A

V

I

A

S

M

S

K

G

C

Y

S

T

F

F

V

V

A

A

R

R

S

G

F

Y

S

A

G

Y

N

D

T

V

P

K

H

H

L

L

V

K

K

E

M

L

I

I

Q

K

L

S

A

A

I

I

Q

K

H

H

K

K

G

G

F

L

A

A

L

L

V

I

D

D

L

V

F

L

S

Q

P

P

C
|
C

V

P

S
x
T

F

Y

N

N

K

D

V

I

N

N

T

T

F

K

G

E

W

W

Y

R

K

I

Q

Y

R

K

C

L

Q

D

E

T

L

L

P

P

E

-

D

D

Y

W

D

D

P

P

T

V

-

V

-

K

-

P

-

E

-

V

-

N

-

E

D

K

W

I

A

K

A

R

A

A

M

I

Q

D

V

K

A

S

Q

L

E

E

F

W

G

G

E

D

R

-

I

I

P

P

I

I

G

G

V

I

I

F

Y

Y

R

Q

N

N

D

E

-

L

-

V

-

P

-

S

-

Y

R

E

P

E

S

R

L

I

D

K

A

A

S

N

L

S

P

P

V

A

L

Y

Q

L

K

D

G

Y

P

T

L

P

G

A

R

K

Q

Q

P

L

V

I

D

E

R

K

D

E

G

G

binding magnesium ion: D84 (= D91), N112 (= N119), V114 (≠ I121)
binding iron/sulfur cluster: C8 (= C15), C11 (= C18), C42 (≠ Q49), C183 (= C198), T185 (≠ S200)
binding 2-[(2E)-3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-(1-oxidanylpropylidene)-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: I40 (= I47), G41 (= G48), C42 (≠ Q49), S43 (≠ A50), H59 (= H66), G85 (= G92), D86 (≠ C93), N112 (= N119), V114 (≠ I121), Y115 (= Y122), G116 (= G123), L117 (= L124)

5b47B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - pyruvate complex (see paper)
37% identity, 93% coverage: 14:275/283 of query aligns to 4:261/275 of 5b47B

query
sites
5b47B

W

W

C
|
C

P

P

G

G

C
|
C

G

G

N

D

H

F

D

G

V

I

M

L

K

R

A

A

V

E

R

E

Q

M

A

A

L

I

A

R

G

E

L

L

D

G

L

I

A

N

P

P

N

K

R

S

V

V

A

V

H

I

V

V

S

S

G

G

I
|
I

G
|
G

Q
x
C

A
x
S

A

G

K

K

A

I

P

P

H

H

Y

F

I

M

D

N

L

L

-

P

-

I

N

S

G

G

F

V

N

H

G

T

L

L

H
|
H

G

G

R

R

G

S

L

I

P

A

P

F

A

A

Q

T

A

G

I

I

K

K

L

L

C

S

N

N

P

P

E

S

L

L

T

E

V

V

I

I

A

V

Q

N

S

V

G

G

D
|
D

G
|
G

C
x
D

N

G

Y

L

G

G

E

I

G

G

G

M

N

G

H

H

F

F

L

V

A

H

A

L

I

G

R

R

N

N

V

I

D

D

M

I

T

A

L

V

L

L

V

V

H

H

D

N

N
|
N

Q

G

I
x
V

Y
|
Y

G

G

L
|
L

T

-

K

-

G

-

Q

Q

A

A

S

S

P

P

T

T

T

L

P

H

E

R

G

G

Q

E

I

-

T

-

K

-

S

N

Q

I

P

M

D

D

G

A

V

V

R

-

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binding magnesium ion: D83 (= D91), N111 (= N119)
binding iron/sulfur cluster: C5 (= C15), C8 (= C18), C39 (≠ Q49), C179 (= C198)
binding thiamine diphosphate: I37 (= I47), G38 (= G48), C39 (≠ Q49), S40 (≠ A50), H58 (= H66), G84 (= G92), D85 (≠ C93), N111 (= N119), V113 (≠ I121), Y114 (= Y122), L116 (= L124)

5exdF Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-di-oxido-methyl-tpp (coom-tpp) intermediate (see paper)
28% identity, 40% coverage: 85:198/283 of query aligns to 104:225/310 of 5exdF

query
sites
5exdF

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active site: N143 (≠ L124)
binding magnesium ion: D110 (= D91), N138 (= N119), S140 (≠ I121)
binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-[1,1,2-tris(oxidanyl)-2-oxidanylidene-ethyl]-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: D110 (= D91), G111 (= G92), Y136 (≠ H117), N138 (= N119), S140 (≠ I121), Y141 (= Y122), A142 (≠ G123), N143 (≠ L124), T144 (= T125)
binding iron/sulfur cluster: A142 (≠ G123), C225 (= C198)

Sites not aligning to the query:

5c4iC Structure of an oxalate oxidoreductase (see paper)
28% identity, 40% coverage: 85:198/283 of query aligns to 104:225/312 of 5c4iC

query
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5c4iC

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active site: N143 (≠ L124)
binding magnesium ion: D110 (= D91), N138 (= N119), S140 (≠ I121)
binding iron/sulfur cluster: A142 (≠ G123), C225 (= C198)
binding thiamine diphosphate: G109 (= G90), D110 (= D91), G111 (= G92), Y136 (≠ H117), N138 (= N119), S140 (≠ I121), Y141 (= Y122), A142 (≠ G123), N143 (≠ L124), T144 (= T125)

Sites not aligning to the query:

binding iron/sulfur cluster: 24, 27, 29, 52, 226, 227
binding thiamine diphosphate: 50, 51, 52, 74

5exeC Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-tpp adduct (see paper)
28% identity, 40% coverage: 85:198/283 of query aligns to 104:225/314 of 5exeC

query
sites
5exeC

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active site: N143 (≠ L124)
binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-carboxy-4-methyl-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: G109 (= G90), D110 (= D91), G111 (= G92), Y136 (≠ H117), N138 (= N119), S140 (≠ I121), Y141 (= Y122), A142 (≠ G123), N143 (≠ L124), T144 (= T125)
binding magnesium ion: D110 (= D91), D130 (= D111), N138 (= N119), S140 (≠ I121), L211 (≠ I184), Q213 (≠ H186)
binding iron/sulfur cluster: C225 (= C198)

Sites not aligning to the query:

Query Sequence

>8499412 FitnessBrowser__Miya:8499412
MDIEVYGEYKKTAWCPGCGNHDVMKAVRQALAGLDLAPNRVAHVSGIGQAAKAPHYIDLN
GFNGLHGRGLPPAQAIKLCNPELTVIAQSGDGCNYGEGGNHFLAAIRRNVDMTLLVHDNQ
IYGLTKGQASPTTPEGQITKSQPDGVRNAPFNPIAVAVAMKCSFVARSFSGNTPHLVKMI
QLAIQHKGFALVDLFSPCVSFNKVNTFGWYKQRCQELPEDYDPTDWAAAMQVAQEFGERI
PIGVIYRNDRPSLDASLPVLQKGPLGRQPVDRDGLRALMQSYT

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory