SitesBLAST

7bkcK Formate dehydrogenase - heterodisulfide reductase - formylmethanofuran dehydrogenase complex from methanospirillum hungatei (dimeric, composite structure) (see paper)
30% identity, 44% coverage: 78:142/147 of query aligns to 63:143/321 of 7bkcK

query
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7bkcK

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binding iron/sulfur cluster: C77 (= C92), S78 (≠ M93), Y79 (≠ H94), C80 (= C95), G81 (= G96), C83 (= C98), C87 (= C102), L92 (= L107), I119 (≠ F118), C124 (= C123), V125 (≠ T124), C127 (= C126), C130 (= C129), C134 (= C133), P135 (= P134), I139 (≠ M138)

Sites not aligning to the query:

binding iron/sulfur cluster: 36, 37, 39, 42, 46, 47, 51, 178, 183, 184, 185, 186, 187, 189, 193, 194, 198, 208, 215, 216, 219, 221, 222, 226, 257, 258, 259, 260, 261, 263, 267, 268

7bkbK Formate dehydrogenase - heterodisulfide reductase - formylmethanofuran dehydrogenase complex from methanospirillum hungatei (hexameric, composite structure) (see paper)
30% identity, 44% coverage: 78:142/147 of query aligns to 63:143/386 of 7bkbK

query
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7bkbK

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binding iron/sulfur cluster: C77 (= C92), S78 (≠ M93), Y79 (≠ H94), C80 (= C95), G81 (= G96), C83 (= C98), C87 (= C102), L92 (= L107), I119 (≠ F118), C124 (= C123), V125 (≠ T124), C127 (= C126), C130 (= C129), C134 (= C133), I139 (≠ M138)

Sites not aligning to the query:

binding iron/sulfur cluster: 36, 37, 39, 42, 46, 47, 51, 173, 174, 175, 176, 179, 183, 186, 211, 212, 214, 215, 217, 221, 222, 241, 248, 249, 251, 254, 258, 259, 263, 265, 273, 280, 281, 284, 286, 287, 291, 322, 323, 324, 325, 326, 328, 332, 333

7ak5I Cryo-em structure of respiratory complex i in the deactive state from mus musculus at 3.2 a (see paper)
34% identity, 38% coverage: 83:138/147 of query aligns to 70:133/178 of 7ak5I

query
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7ak5I

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binding iron/sulfur cluster: C79 (= C92), I80 (≠ M93), A81 (≠ H94), C82 (= C95), K83 (≠ G96), C85 (= C98), C89 (= C102), C118 (= C123), I119 (≠ T124), Y120 (≠ A125), C121 (= C126), G122 (= G127), C124 (= C129), C128 (= C133), I133 (≠ M138)

Sites not aligning to the query:

binding iron/sulfur cluster: 67, 135

8e73S8 qcr9 (see paper)
34% identity, 38% coverage: 83:138/147 of query aligns to 73:136/181 of 8e73S8

query
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8e73S8

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binding iron/sulfur cluster: C82 (= C92), I83 (≠ M93), C85 (= C95), K86 (≠ G96), C88 (= C98), C92 (= C102), I97 (≠ L107), C121 (= C123), I122 (≠ T124), Y123 (≠ A125), C124 (= C126), G125 (= G127), F126 (≠ L128), C127 (= C129), C131 (= C133), P132 (= P134), A135 (= A137), I136 (≠ M138)

Sites not aligning to the query:

binding iron/sulfur cluster: 70, 138

8a8oD Paps reductase from methanothermococcus thermolithotrophicus refined to 1.45 a (see paper)
37% identity, 39% coverage: 89:146/147 of query aligns to 6:65/102 of 8a8oD

query
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8a8oD

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binding iron/sulfur cluster: C9 (= C92), I10 (≠ M93), G11 (≠ H94), C12 (= C95), G13 (= G96), C15 (= C98), C19 (= C102), P20 (= P103), S32 (≠ V115), C40 (= C123), W41 (≠ T124), D42 (≠ A125), C43 (= C126), A44 (≠ G127), C46 (= C129), C50 (= C133), I55 (vs. gap)

Sites not aligning to the query:

binding iron/sulfur cluster: 2

7arcI Cryo-em structure of polytomella complex-i (peripheral arm) (see paper)
34% identity, 38% coverage: 83:138/147 of query aligns to 91:154/199 of 7arcI

query
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7arcI

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binding iron/sulfur cluster: C100 (= C92), I101 (≠ M93), S102 (≠ H94), C103 (= C95), K104 (≠ G96), C106 (= C98), C110 (= C102), P111 (= P103), I115 (≠ L107), Y132 (≠ F118), C139 (= C123), I140 (≠ T124), Y141 (≠ A125), C142 (= C126), G143 (= G127), F144 (≠ L128), C145 (= C129), C149 (= C133), A153 (= A137), I154 (≠ M138)
binding : R131 (≠ V117)

Sites not aligning to the query:

binding iron/sulfur cluster: 88, 156
binding : 168, 169, 171, 172, 173

1durA Replacement for 1fdx 2(4fe4s) ferredoxin from (now) peptostreptococcus asaccharolyticus
44% identity, 35% coverage: 90:141/147 of query aligns to 6:54/55 of 1durA

query
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1durA

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binding iron/sulfur cluster: C8 (= C92), I9 (≠ M93), A10 (≠ H94), C11 (= C95), G12 (= G96), C14 (= C98), C18 (= C102), C22 (≠ A106), I23 (≠ L107), Y29 (≠ V116), I31 (≠ F118), C36 (= C123), I37 (≠ T124), C39 (= C126), G40 (= G127), C42 (= C129), C46 (= C133), P47 (= P134)

Sites not aligning to the query:

binding iron/sulfur cluster: 2, 4

8b9zI Drosophila melanogaster complex i in the active state (dm1) (see paper)
34% identity, 38% coverage: 83:138/147 of query aligns to 78:141/186 of 8b9zI

query
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8b9zI

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binding iron/sulfur cluster: C87 (= C92), I88 (≠ M93), A89 (≠ H94), C90 (= C95), K91 (≠ G96), C93 (= C98), C97 (= C102), P98 (= P103), Y119 (≠ F118), C126 (= C123), I127 (≠ T124), Y128 (≠ A125), C129 (= C126), G130 (= G127), F131 (≠ L128), C132 (= C129), C136 (= C133), A140 (= A137), I141 (≠ M138)

Sites not aligning to the query:

binding iron/sulfur cluster: 75, 143

Q9VF27 NADH dehydrogenase (ubiquinone) 23 kDa subunit; EC 7.1.1.2 from Drosophila melanogaster (Fruit fly) (see paper)
34% identity, 38% coverage: 83:138/147 of query aligns to 109:172/217 of Q9VF27

query
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Q9VF27

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Sites not aligning to the query:

199 G→D: Disrupts mitochondrial function and results in enlarged mitochondria. Neurons present vacuolar lesions leading to neurodegeneration in the central brain. Results in behavioral defects and shorten lifespan.

5exdH Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-di-oxido-methyl-tpp (coom-tpp) intermediate (see paper)
41% identity, 38% coverage: 83:138/147 of query aligns to 228:281/291 of 5exdH

query
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5exdH

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binding iron/sulfur cluster: P230 (≠ I85), C237 (= C92), T238 (≠ M93), E239 (≠ H94), C240 (= C95), Y241 (≠ G96), C243 (= C98), C247 (= C102), P259 (≠ R114), C266 (= C123), K267 (≠ T124), G268 (≠ A125), C269 (= C126), G270 (= G127), C272 (= C129), C276 (= C133), S278 (≠ V135), L281 (≠ M138)

7zm7I Cryoem structure of mitochondrial complex i from chaetomium thermophilum (inhibited by ddm) (see paper)
34% identity, 43% coverage: 83:145/147 of query aligns to 77:146/185 of 7zm7I

query
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7zm7I

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binding iron/sulfur cluster: C86 (= C92), I87 (≠ M93), A88 (≠ H94), C89 (= C95), K90 (≠ G96), C92 (= C98), C96 (= C102), P97 (= P103), I101 (≠ L107), Y118 (≠ F118), C125 (= C123), I126 (≠ T124), Y127 (≠ A125), C128 (= C126), C131 (= C129), C135 (= C133), A139 (= A137), I140 (≠ M138)

Sites not aligning to the query:

binding iron/sulfur cluster: 74

5c4iE Structure of an oxalate oxidoreductase (see paper)
41% identity, 38% coverage: 83:138/147 of query aligns to 249:302/312 of 5c4iE

query
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5c4iE

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binding iron/sulfur cluster: C258 (= C92), T259 (≠ M93), E260 (≠ H94), C261 (= C95), Y262 (≠ G96), C264 (= C98), C268 (= C102), C272 (≠ A106), C287 (= C123), K288 (≠ T124), G289 (≠ A125), C290 (= C126), G291 (= G127), C293 (= C129), C297 (= C133), P298 (= P134), S299 (≠ V135), L302 (≠ M138)

1clfA Clostridium pasteurianum ferredoxin (see paper)
46% identity, 33% coverage: 90:137/147 of query aligns to 6:51/55 of 1clfA

query
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1clfA

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V

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I

D

D

K

A

D

D

R

T

C
|
C

T
x
I

A

D

C
|
C

G

G

L
x
N

C
|
C

T

A

R

N

V

V

C
|
C

P

P

V

V

G

G

A
|
A

binding iron/sulfur cluster: C8 (= C92), V9 (≠ M93), C11 (= C95), G12 (= G96), A13 (≠ M97), C14 (= C98), C18 (= C102), P19 (= P103), V20 (≠ T104), F30 (≠ V116), C37 (= C123), I38 (≠ T124), C40 (= C126), N42 (≠ L128), C43 (= C129), C47 (= C133), A51 (= A137)

Sites not aligning to the query:

binding iron/sulfur cluster: 4

P00195 Ferredoxin from Clostridium pasteurianum (see 2 papers)
46% identity, 33% coverage: 90:137/147 of query aligns to 7:52/56 of P00195

query
sites
P00195

D

D

S

S

C
|
C

M

V

H

S

C

C

G

G

M

A

C

C

T

A

A

S

I

E

C

C

P

P

T

V

S

N

A

A

L

I

A

S

M

Q

D

G

I

-

E

-

A

D

R

S

V

I

V

F

V

V

F

I

D

D

K

A

D

D

R

T

C

C

T

I

A

D

C

C

G

G

L

N

C

C

T

A

R

N

V

V

C

C

P

P

V

V

G

G

A

A

C9 (= C92) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

5gupH structure of mammalian respiratory supercomplex I1III2IV1 (see paper)
33% identity, 38% coverage: 83:138/147 of query aligns to 68:131/176 of 5gupH

query
sites
5gupH

Q

R

R

R

I

Y

S

P

R

S

D

G

E

E

D

E

S

R

C
|
C

M
x
I

H
x
A

C
|
C

G
x
K

M

L

C
|
C

T

E

A

A

I

V

C
|
C

P

P

T

A

S

Q

A

A

L

I

A

T

M

I

D

E

I

A

E

E

A

P

R

R

V

A

-

D

-

G

-

S

-

R

-

T

V

T

V
x
R

F

Y

D

D

K

I

D

D

-

M

-

T

R

K

C
|
C

T
x
I

A
x
Y

C
|
C

G
|
G

L
x
F

C
|
C

T

Q

R

E

V

A

C
|
C

P
|
P

V

V

G

D

A
|
A

M
x
I

binding iron/sulfur cluster: C77 (= C92), I78 (≠ M93), A79 (≠ H94), C80 (= C95), K81 (≠ G96), C83 (= C98), C87 (= C102), C116 (= C123), I117 (≠ T124), Y118 (≠ A125), C119 (= C126), G120 (= G127), F121 (≠ L128), C122 (= C129), C126 (= C133), P127 (= P134), A130 (= A137), I131 (≠ M138)
binding : R108 (≠ V117)

Sites not aligning to the query:

8b6fAL ndutt15 (see paper)
33% identity, 38% coverage: 83:138/147 of query aligns to 109:172/218 of 8b6fAL

query
sites
8b6fAL

Q

R

R

R

I

Y

S

P

R

T

D

G

E

E

D

E

S

R

C
|
C

M
x
I

H

A

C
|
C

G
x
K

M

L

C
|
C

T

Q

A

S

I

A

C
|
C

P

P

T

A

S

R

A

A

L
x
I

A

T

M

I

D

E

I

T

E

E

A

P

R

R

-

P

-

D

-

G

-

S

-

R

V

T

V

V

V

R

F
x
Y

D

D

K

I

D

D

-

M

-

T

R

K

C
|
C

T
x
I

A
x
Y

C
|
C

G
|
G

L

F

C
|
C

T

Q

R

E

V

A

C
|
C

P
|
P

V

V

G

D

A
|
A

M
x
I

binding iron/sulfur cluster: C118 (= C92), I119 (≠ M93), C121 (= C95), K122 (≠ G96), C124 (= C98), C128 (= C102), I133 (≠ L107), Y150 (≠ F118), C157 (= C123), I158 (≠ T124), Y159 (≠ A125), C160 (= C126), G161 (= G127), C163 (= C129), C167 (= C133), P168 (= P134), A171 (= A137), I172 (≠ M138)

Sites not aligning to the query:

binding iron/sulfur cluster: 106, 174

8a6tB Cryo-em structure of the electron bifurcating fe-fe hydrogenase hydabc complex from thermoanaerobacter kivui in the reduced state (see paper)
35% identity, 35% coverage: 88:138/147 of query aligns to 578:627/630 of 8a6tB

query
sites
8a6tB

D

D

E

P

D

G

S

K

C
|
C

M
x
I

H

G

C
|
C

G

G

M

K

C
|
C

T

V

A

K

I

V

C
|
C

P

P

T

V

S

G

A
|
A

L
x
I

A

S

M

G

D

E

I

-

E

K

A

K

R

K

V

P

V

H

V

V

F
x
I

D

D

K

Q

D

S

R

K

C
|
C

T

I

A

K

C

C

G

G

L

A

C
|
C

T

A

R

E

V

N

C
|
C

P

P

V
x
K

G

G

A
|
A

M
x
I

binding iron/sulfur cluster: C582 (= C92), I583 (≠ M93), C585 (= C95), C588 (= C98), C592 (= C102), A596 (= A106), I597 (≠ L107), I607 (≠ F118), C612 (= C123), C618 (= C129), C622 (= C133), K624 (≠ V135), A626 (= A137), I627 (≠ M138)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 31, 33, 36, 82, 85, 86
binding flavin mononucleotide: 201, 227, 230, 355, 535, 536
binding nadp nicotinamide-adenine-dinucleotide phosphate: 320, 337, 340, 341, 342, 433
binding iron/sulfur cluster: 487, 488, 489, 491, 494, 534, 536, 537, 575, 577
binding zinc ion: 471, 558, 564

Query Sequence

>8499492 FitnessBrowser__Miya:8499492
MTEQQTKPYRKNIHLTFPPEISGKPIVSDLVRRYDLTVNILKAQITPRKEGYLTLEISGG
EDNCLKGIAYLREQDVTVTDVSQRISRDEDSCMHCGMCTAICPTSALAMDIEARVVVFDK
DRCTACGLCTRVCPVGAMNVEVENGGL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory