SitesBLAST
Comparing 8499676 FitnessBrowser__Miya:8499676 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 18 hits to proteins with known functional sites (download)
2y27B Crystal structure of paak1 in complex with atp from burkholderia cenocepacia (see paper)
56% identity, 98% coverage: 8:431/434 of query aligns to 8:427/427 of 2y27B
- binding adenosine-5'-triphosphate: K65 (= K65), S90 (= S90), S91 (= S91), G92 (= G92), T93 (= T93), T94 (= T94), F138 (= F138), A211 (= A211), E212 (= E212), P213 (= P213), D232 (≠ N232), I233 (= I233), Y234 (= Y234), G235 (= G235), L236 (= L236), S237 (= S237), D302 (= D302), I320 (≠ V323), R323 (= R326), K419 (= K423)
- binding magnesium ion: V200 (≠ R200), S202 (≠ L202), L204 (= L204), M226 (≠ L226), G227 (= G227), Q347 (≠ T350), L350 (= L353)
2y4oA Crystal structure of paak2 in complex with phenylacetyl adenylate (see paper)
56% identity, 98% coverage: 8:432/434 of query aligns to 10:433/433 of 2y4oA
- binding 5'-o-[hydroxy(phenylacetyl)phosphoryl]adenosine: F135 (≠ Y133), F140 (= F138), A213 (= A211), E214 (= E212), P215 (= P213), I235 (= I233), G237 (= G235), L238 (= L236), S239 (= S237), P244 (= P242), D304 (= D302), R325 (= R326), I331 (= I332), N336 (= N337)
2y4oB Crystal structure of paak2 in complex with phenylacetyl adenylate (see paper)
56% identity, 98% coverage: 8:431/434 of query aligns to 10:432/432 of 2y4oB
- binding 5'-o-[hydroxy(phenylacetyl)phosphoryl]adenosine: F135 (≠ Y133), F140 (= F138), G212 (= G210), A213 (= A211), E214 (= E212), P215 (= P213), I235 (= I233), G237 (= G235), L238 (= L236), S239 (= S237), P244 (= P242), D304 (= D302), R325 (= R326), I331 (= I332), N336 (= N337)
- binding magnesium ion: S204 (≠ L202), V228 (≠ L226)
2y4nA Paak1 in complex with phenylacetyl adenylate (see paper)
56% identity, 98% coverage: 8:432/434 of query aligns to 8:426/426 of 2y4nA
- binding 5'-o-[hydroxy(phenylacetyl)phosphoryl]adenosine: Y131 (= Y133), F136 (= F138), G138 (= G140), G208 (= G210), A209 (= A211), E210 (= E212), P211 (= P213), I231 (= I233), Y232 (= Y234), G233 (= G235), L234 (= L236), S235 (= S237), P240 (= P242), D300 (= D302), R321 (= R326), K417 (= K423)
- binding magnesium ion: V198 (≠ R200), S200 (≠ L202), Q345 (≠ T350), L348 (= L353)
4r1mA Crystal structure of a putative acyl-coa ligase (bt_0428) from bacteroides thetaiotaomicron vpi-5482 at 2.48 a resolution
46% identity, 98% coverage: 8:433/434 of query aligns to 12:435/435 of 4r1mA
- binding adenosine monophosphate: A215 (= A211), E216 (= E212), P217 (= P213), N236 (= N232), S237 (≠ I233), F238 (≠ Y234), G239 (= G235), M240 (≠ L236), T241 (≠ S237), D305 (= D302), R329 (= R326), I335 (= I332), N340 (= N337)
- binding zinc ion: C252 (= C248), H259 (= H256), C314 (= C311), C316 (= C313)
4r1lA Crystal structure of a putative acyl-coa ligase (bt_0428) from bacteroides thetaiotaomicron vpi-5482 at 2.42 a resolution
46% identity, 98% coverage: 8:433/434 of query aligns to 12:433/433 of 4r1lA
- binding adenosine-5'-diphosphate: A215 (= A211), E216 (= E212), P217 (= P213), S237 (≠ I233), F238 (≠ Y234), G239 (= G235), M240 (≠ L236), T241 (≠ S237), D305 (= D302), R329 (= R326), N340 (= N337)
- binding adenosine monophosphate: A215 (= A211), E216 (= E212), P217 (= P213), S237 (≠ I233), F238 (≠ Y234), G239 (= G235), M240 (≠ L236), T241 (≠ S237), D305 (= D302), R329 (= R326), N340 (= N337)
- binding coenzyme a: S136 (≠ A132), A164 (≠ G160), G165 (= G161), N166 (≠ A162), S167 (≠ T163), I185 (≠ T181), Y188 (= Y184), K337 (≠ R334), T408 (= T406)
- binding zinc ion: C252 (= C248), H259 (= H256), C314 (= C311), C316 (= C313)
6he0A Crystal structure of 2-hydroxyisobutyryl-coa ligase (hcl) in complex with 2-hib-amp and coa in the thioesterfication state (see paper)
34% identity, 98% coverage: 7:432/434 of query aligns to 29:465/477 of 6he0A
- binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] 2-methyl-2-oxidanyl-propanoate: S241 (≠ G210), G242 (≠ A211), E243 (= E212), P244 (= P213), G267 (≠ Y234), S268 (≠ G235), M269 (≠ L236), A270 (≠ S237), D335 (= D302), I357 (≠ V323), N371 (= N337)
- binding adenosine monophosphate: G242 (≠ A211), E243 (= E212), P244 (= P213), C266 (≠ I233), G267 (≠ Y234), S268 (≠ G235), A270 (≠ S237), E271 (= E238), D335 (= D302), N371 (= N337)
- binding coenzyme a: Y166 (≠ F138), A188 (≠ G160), G189 (= G161), P191 (vs. gap), S194 (≠ T163), Y210 (≠ C179), G211 (≠ C180), T212 (= T181), Y215 (= Y184), H218 (≠ Y187), R368 (= R334), G369 (= G335), M401 (≠ L367), V439 (= V405), R440 (≠ T406)
6hdyA Crystal structure of 2-hydroxyisobutyryl-coa ligase (hcl) in the postadenylation state in complex with s3-hb-amp (see paper)
33% identity, 98% coverage: 7:432/434 of query aligns to 29:462/474 of 6hdyA
- binding (3s)-3-hydroxybutanoic acid: Y162 (≠ F138), S237 (≠ G210), G263 (≠ Y234), S264 (≠ G235), M265 (≠ L236), A266 (≠ S237), F271 (≠ G243)
- binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] (3~{S})-3-oxidanylbutanoate: Y162 (≠ F138), G164 (= G140), S237 (≠ G210), G238 (≠ A211), E239 (= E212), P240 (= P213), C262 (≠ I233), G263 (≠ Y234), S264 (≠ G235), A266 (≠ S237), F271 (≠ G243), D331 (= D302), I353 (≠ V323), R356 (= R326), K453 (= K423)
6hdxA Crystal structure of 2-hydroxyisobutyryl-coa ligase (hcl) in the postadenylation state in complex with r3-hib-amp (see paper)
33% identity, 98% coverage: 7:432/434 of query aligns to 29:462/474 of 6hdxA
- binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] (2~{R})-2-methyl-3-oxidanyl-propanoate: Y162 (≠ F138), G164 (= G140), S237 (≠ G210), G238 (≠ A211), E239 (= E212), P240 (= P213), C262 (≠ I233), G263 (≠ Y234), S264 (≠ G235), A266 (≠ S237), F271 (≠ G243), D331 (= D302), I353 (≠ V323), R356 (= R326), K453 (= K423)
- binding (2r)-3-hydroxy-2-methylpropanoic acid: Y162 (≠ F138), G164 (= G140), S237 (≠ G210), G263 (≠ Y234), S264 (≠ G235), A266 (≠ S237), F271 (≠ G243)
6siwA Paak family amp-ligase with amp (see paper)
30% identity, 86% coverage: 14:388/434 of query aligns to 12:378/432 of 6siwA
- binding adenosine monophosphate: S84 (= S91), A208 (= A211), E209 (= E212), P210 (= P213), E231 (≠ I233), Y232 (= Y234), G233 (= G235), S234 (≠ L236), T235 (≠ S237), D295 (= D302), V315 (= V323)
- binding magnesium ion: E75 (≠ D82), L77 (≠ I84), S83 (= S90), P116 (≠ V123), G143 (= G152), T145 (= T154), E236 (= E238)
- binding zinc ion: C244 (= C248), H250 (= H256), C304 (= C311), C306 (= C313)
6sixB Paak family amp-ligase with anp (see paper)
30% identity, 86% coverage: 14:388/434 of query aligns to 17:383/437 of 6sixB
- binding phosphoaminophosphonic acid-adenylate ester: S88 (= S90), S89 (= S91), A213 (= A211), E214 (= E212), P215 (= P213), E236 (≠ I233), Y237 (= Y234), G238 (= G235), S239 (≠ L236), T240 (≠ S237), E241 (= E238), D300 (= D302), V320 (= V323), R323 (= R326)
- binding magnesium ion: R79 (≠ K81), E80 (≠ D82), P121 (≠ V123), T150 (= T154)
- binding zinc ion: C249 (= C248), H255 (= H256), C309 (= C311), C311 (= C313)
6siyA Paak family amp-ligase with amp and substrate (see paper)
30% identity, 86% coverage: 14:388/434 of query aligns to 13:379/433 of 6siyA
- binding 3-hydroxyanthranilic acid: T125 (≠ N131), P126 (≠ A132), T132 (= T139), L135 (= L142), R153 (≠ G161), N177 (≠ T181), A209 (= A211), E232 (≠ I233), G234 (= G235), S235 (≠ L236)
- binding adenosine monophosphate: S85 (= S91), A209 (= A211), E210 (= E212), P211 (= P213), E232 (≠ I233), Y233 (= Y234), G234 (= G235), S235 (≠ L236), T236 (≠ S237), D296 (= D302), V316 (= V323)
- binding magnesium ion: R75 (≠ K81), E76 (≠ D82), L78 (≠ I84), P117 (≠ V123), G144 (= G152), A145 (= A153), T146 (= T154)
Q04747 Surfactin synthase subunit 2 from Bacillus subtilis (strain 168) (see paper)
27% identity, 71% coverage: 90:399/434 of query aligns to 1645:1960/3583 of Q04747
Sites not aligning to the query:
- 999 modified: O-(pantetheine 4'-phosphoryl)serine
- 2040 modified: O-(pantetheine 4'-phosphoryl)serine
Q84P21 Peroxisomal OPC-8:0-CoA ligase 1; 4-coumarate--CoA ligase isoform 9; At4CL9; 4-coumarate--CoA ligase-like 5; EC 6.2.1.- from Arabidopsis thaliana (Mouse-ear cress) (see paper)
23% identity, 62% coverage: 79:348/434 of query aligns to 186:461/546 of Q84P21
Sites not aligning to the query:
- 530 K→N: Lossed enzymatic activity.
3pbkA Structural and functional studies of fatty acyl-adenylate ligases from e. Coli and l. Pneumophila (see paper)
37% identity, 24% coverage: 323:426/434 of query aligns to 443:541/555 of 3pbkA
Sites not aligning to the query:
- active site: 171, 191, 216, 327, 328
- binding 5'-O-[(S)-(dodecanoyloxy)(hydroxy)phosphoryl]adenosine: 217, 222, 295, 296, 297, 323, 324, 325, 326, 327, 331, 432
3cw9A 4-chlorobenzoyl-coa ligase/synthetase in the thioester-forming conformation, bound to 4-chlorophenacyl-coa (see paper)
27% identity, 43% coverage: 169:353/434 of query aligns to 239:427/503 of 3cw9A
- active site: T307 (≠ S237), E308 (= E238), I406 (= I332), N411 (= N337)
- binding 4-Chlorophenacyl-coenzyme A: A280 (≠ G210), G305 (= G235), T306 (≠ L236), M310 (= M240), N311 (≠ G241), S407 (≠ I333), G408 (≠ R334), G409 (= G335), E410 (≠ V336)
- binding adenosine monophosphate: G281 (≠ A211), A282 (≠ E212), T283 (≠ P213), I303 (= I233), Y304 (= Y234), G305 (= G235), T306 (≠ L236), T307 (≠ S237), D385 (= D302), R400 (= R326), I406 (= I332), N411 (= N337)
Sites not aligning to the query:
- active site: 161, 181, 207, 492
- binding 4-Chlorophenacyl-coenzyme A: 87, 203, 204, 207, 208, 209, 440, 473, 475, 477
- binding adenosine monophosphate: 161
1t5dX 4-chlorobenzoyl-coa ligase/synthetase bound to 4-chlorobenzoate (see paper)
27% identity, 43% coverage: 169:353/434 of query aligns to 237:425/502 of 1t5dX
Sites not aligning to the query:
3cw8X 4-chlorobenzoyl-coa ligase/synthetase, bound to 4cba-adenylate (see paper)
27% identity, 43% coverage: 169:353/434 of query aligns to 239:427/501 of 3cw8X
- binding 5'-O-[(S)-{[(4-chlorophenyl)carbonyl]oxy}(hydroxy)phosphoryl]adenosine: G281 (≠ A211), A282 (≠ E212), T283 (≠ P213), I303 (= I233), Y304 (= Y234), G305 (= G235), T306 (≠ L236), T307 (≠ S237), M310 (= M240), N311 (≠ G241), M324 (≠ I257), D385 (= D302)
Sites not aligning to the query:
Query Sequence
>8499676 FitnessBrowser__Miya:8499676
MIFDVDRETLPREELEALQLRRLKNLCERVYANVPHYRRRFEEAGVTPADIKRLSDVTLL
PFTEKQDLRNNYPFGLFAVPKDTIVRLHASSGTTGKATVVGYTKRDLDNWAELMARSLAA
AGVTRRDLIHNAYGYGLFTGGLGAHYGAERLGATVIPVSGGATKRQVLLLRDFGATVICC
TPSYSLYLHEAAEEAGIDLRELPLRIGVFGAEPWSEEMRAEIETKLGIDALNIYGLSEIM
GPGVSMECVEAKCGMHIYEDHFLPEIIDPVSGEALPPGATGELVITTLTKEGIPLVRYRT
RDITSLDYTPCACGRTHVRMRRVTGRSDDMLIIRGVNVFPSQIESILMETEGLAPHYQLL
VTREGNLDNMEVQVEVSEEFFSDEIKNLQRREARLQKTIKEFLGVTAKVRLVEPRSIQRS
EGKAKRVIDMREKS
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory