SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 8499708 FitnessBrowser__Miya:8499708 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 9 hits to proteins with known functional sites (download)

6addA The crystal structure of rv2747 from mycobacterium tuberculosis in complex with coa and nlq (see paper)
37% identity, 85% coverage: 3:134/155 of query aligns to 3:134/168 of 6addA

query
sites
6addA

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binding coenzyme a: Y24 (≠ T24), I28 (≠ L29), V75 (≠ L75), A76 (≠ V76), V77 (= V77), T82 (≠ R82), G83 (= G83), H84 (≠ K84), G85 (= G85), G87 (= G87), H88 (≠ R88), T110 (= T110), E112 (≠ Q112), F115 (= F115)
binding n~2~-acetyl-l-glutamine: K27 (≠ Q27), I28 (≠ L29), L30 (= L31), T74 (≠ S74), L109 (= L109)

Sites not aligning to the query:

5yo2A The crystal structure of rv2747 from mycobacterium tuberculosis in complex with acetyl coa and l-arginine (see paper)
37% identity, 85% coverage: 3:134/155 of query aligns to 3:134/168 of 5yo2A

query
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5yo2A

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binding acetyl coenzyme *a: Y24 (≠ T24), I28 (≠ L29), I72 (≠ V72), R73 (= R73), T74 (≠ S74), V75 (≠ L75), V77 (= V77), T82 (≠ R82), G83 (= G83), H84 (≠ K84), G85 (= G85), G87 (= G87), H88 (≠ R88), T110 (= T110), E112 (≠ Q112), F115 (= F115), F116 (= F116)
binding arginine: G26 (= G26), L29 (= L30), L30 (= L31), R73 (= R73), L109 (= L109)

Sites not aligning to the query:

binding arginine: 147

5ygeA Arga complexed with acecoa and glutamate (see paper)
37% identity, 85% coverage: 3:134/155 of query aligns to 4:135/169 of 5ygeA

query
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binding acetyl coenzyme *a: Y25 (≠ T24), I73 (≠ V72), V76 (≠ L75), A77 (≠ V76), V78 (= V77), T83 (≠ R82), G84 (= G83), H85 (≠ K84), G86 (= G85), G88 (= G87), H89 (≠ R88), T111 (= T110), E113 (≠ Q112), F117 (= F116)
binding glutamic acid: K33 (≠ R33), E44 (≠ D44), H64 (≠ S63), R74 (= R73)

Sites not aligning to the query:

binding acetyl coenzyme *a: 1, 2

4i49A Structure of ngnags bound with bisubstrate analog coa-NAG (see paper)
35% identity, 85% coverage: 6:136/155 of query aligns to 277:407/424 of 4i49A

query
sites
4i49A

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binding (2S)-2-({(3S,5R,9R)-1-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]-3,5,9-trihydroxy-8,8-dimethyl-3,5-dioxido-10,14,20-trioxo-2,4,6-trioxa-18-thia-11,15-diaza-3lambda~5~,5lambda~5~-diphosphaicosan-20-yl}amino)pentanedioic acid (non-preferred name): I300 (≠ L29), L301 (= L30), L302 (= L31), R304 (= R33), A343 (≠ R73), C344 (≠ S74), L345 (= L75), A346 (≠ V76), V347 (= V77), Q352 (≠ R82), D353 (≠ G83), G355 (= G85), G357 (= G87), E358 (≠ R88), L379 (= L109), S380 (≠ T110), T383 (≠ V113), W386 (≠ F115), F387 (= F116)

Sites not aligning to the query:

binding (2S)-2-({(3S,5R,9R)-1-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]-3,5,9-trihydroxy-8,8-dimethyl-3,5-dioxido-10,14,20-trioxo-2,4,6-trioxa-18-thia-11,15-diaza-3lambda~5~,5lambda~5~-diphosphaicosan-20-yl}amino)pentanedioic acid (non-preferred name): 413, 415

3d2mA Crystal structure of n-acetylglutamate synthase from neisseria gonorrhoeae complexed with coenzyme a and l-glutamate (see paper)
35% identity, 85% coverage: 6:136/155 of query aligns to 277:407/424 of 3d2mA

query
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3d2mA

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binding coenzyme a: L345 (= L75), V347 (= V77), Q352 (≠ R82), D353 (≠ G83), G355 (= G85), G357 (= G87), E358 (≠ R88), S380 (≠ T110), T383 (≠ V113), W386 (≠ F115)
binding glutamic acid: L302 (= L31), R304 (= R33), A343 (≠ R73), C344 (≠ S74), L379 (= L109)

Sites not aligning to the query:

active site: 26
binding glutamic acid: 413, 415

3b8gA Crysta structure of n-acetylglutamate synthase from neisseria gonorrhoeae complexed with coenzyme a and n-acetyl-glutamate (see paper)
35% identity, 85% coverage: 6:136/155 of query aligns to 277:407/424 of 3b8gA

query
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3b8gA

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binding coenzyme a: I300 (≠ L29), L345 (= L75), V347 (= V77), Q352 (≠ R82), D353 (≠ G83), G355 (= G85), Y356 (≠ W86), G357 (= G87), E358 (≠ R88), S380 (≠ T110), T383 (≠ V113), E385 (= E114), W386 (≠ F115)
binding n-acetyl-l-glutamate: L302 (= L31), R304 (= R33), A343 (≠ R73), C344 (≠ S74), L379 (= L109)

Sites not aligning to the query:

active site: 26
binding n-acetyl-l-glutamate: 413, 415

2r8vA Native structure of n-acetylglutamate synthase from neisseria gonorrhoeae (see paper)
35% identity, 85% coverage: 6:136/155 of query aligns to 277:407/424 of 2r8vA

query
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2r8vA

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binding acetyl coenzyme *a: I300 (≠ L29), L301 (= L30), L345 (= L75), V347 (= V77), Q352 (≠ R82), D353 (≠ G83), G355 (= G85), G357 (= G87), E358 (≠ R88), T383 (≠ V113), E385 (= E114), W386 (≠ F115), F387 (= F116)

Sites not aligning to the query:

active site: 26

3d2pA Crystal structure of n-acetylglutamate synthase from neisseria gonorrhoeae complexed with coenzyme a and l-arginine (see paper)
33% identity, 85% coverage: 6:136/155 of query aligns to 285:411/424 of 3d2pA

query
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3d2pA

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S

-

G

-

Q

-

G

-

L

I

L

L

L

L

P

H

R

R

S

S

L

R

N

E

Q

Y

L

L

Y

E

S

N

H

H

L

I

R

S

D

E

F

F

L

S

V

I

I

L

D

E

P

H

D

D

D

-

G

-

G

G

P

N

L

L

V

Y

G

G

C

C

C

A

A

A

L

L

-

K

S

T

I

F

A

A

W

E

D

A

D

D

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C

A

G

E

E

V

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C

L
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L

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V

A

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D

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D

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L

A

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x
Q

G
x
D

K

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G
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G

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Y

G
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G

R
x
E

K

R

L

L

V

L

D

A

A

H

C

I

L

I

S

D

D

K

A

A

V

R

T

G

L

I

G

G

I

I

F

S

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R

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F

A

A

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L

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Y

T

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E
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E

F
x
W

F

F

L

A

R

E

M

R

G

G

F

F

R

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T

V

A

E

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K

E

D

D

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L

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P

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K

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D

binding coenzyme a: L303 (≠ T24), L349 (= L75), V351 (= V77), Q356 (≠ R82), D357 (≠ G83), G359 (= G85), G361 (= G87), E362 (≠ R88), E389 (= E114), W390 (≠ F115)

Sites not aligning to the query:

active site: 26
binding arginine: 13, 197, 216, 253, 266, 267, 269, 270, 271, 274, 276

P0A6C5 Amino-acid acetyltransferase; N-acetylglutamate synthase; AGS; NAGS; EC 2.3.1.1 from Escherichia coli (strain K12) (see paper)
30% identity, 81% coverage: 6:131/155 of query aligns to 298:423/443 of P0A6C5

query
sites
P0A6C5

I

I

R

R

K

R

A

A

R

T

V

I

Q

N

D

D

V

I

K

G

P

G

I

I

H

L

A

E

L

L

L

I

M

R

H

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T

L

S

E

G

Q

Q

Q

G

G

L

I

L

L

L

V

P

R

R

R

S

S

L

R

N

E

Q

Q

L

L

Y

E

S

M

H

E

L

I

R

D

D

K

F

F

L

T

V

I

I

I

D

Q

P

R

D

D

D

N

G

-

G

-

P

T

L

T

V

I

G

A

C

C

C

A

A

A

L

L

-

Y

S

P

I

F

A

P

W

E

D

E

D

K

I

I

A

G

E

E

V

M

R

A

S

C

L

V

V

A

V

V

A

H

D

P

D

D

L

Y

R

R

G

S

K

S

G

S

W

R

G

G

R

E

K

V

L

L

V

L

D

E

A

R

C

I

L

A

S

A

D

Q

A

A

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K

T

Q

L

S

G

G

I

L

F

S

R

K

V

L

F

F

A

V

L

L

T

T

Y

T

Q

R

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S

V

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H

F

W

F

F

L

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M

R

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G

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F

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V

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K

I

D

D

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L

L

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E

Sites not aligning to the query:

15 H→Y: In EE17.
19 Y→C: In EE51.
54 S→N: In PT2M217.
58 R→H: In EE11.
287 G→S: In PT2M216.
432 Q→R: In PT2M217.

Query Sequence

>8499708 FitnessBrowser__Miya:8499708
MGQPYIRKARVQDVKPIHALLMHTSGQGLLLPRSLNQLYSHLRDFLVIDPDDGGPLVGCC
ALSIAWDDIAEVRSLVVADDLRGKGWGRKLVDACLSDAVTLGIFRVFALTYQVEFFLRMG
FRVVEKDVLPQKIWADCIHCPKFPDCDETAVLLEM

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory