SitesBLAST

P50870 Streptogramin A acetyltransferase; Virginiamycin acetyltransferase D; Vat(D); EC 2.3.1.- from Enterococcus faecium (Streptococcus faecium) (see paper)
45% identity, 27% coverage: 136:188/194 of query aligns to 116:170/209 of P50870

query
sites
P50870

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Sites not aligning to the query:

82 H→A: 105-fold decrease in activity.

3dhoA Structure of streptogramin acetyltransferase in complex with an inhibitor
45% identity, 27% coverage: 136:188/194 of query aligns to 116:170/203 of 3dhoA

query
sites
3dhoA

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binding 6-bromo-N'-[(1Z)-(3,5-dichloro-2-hydroxyphenyl)methylidene]-2-methylquinoline-4-carbohydrazide: I139 (≠ V157), A141 (= A159), V147 (≠ A165), L155 (≠ I173), G157 (≠ A175), P160 (= P178), I164 (≠ L182)

Sites not aligning to the query:

binding 6-bromo-N'-[(1Z)-(3,5-dichloro-2-hydroxyphenyl)methylidene]-2-methylquinoline-4-carbohydrazide: 84

4isxA The crystal structure of maltose o-acetyltransferase from clostridium difficile 630 in complex with acetyl-coa
30% identity, 55% coverage: 79:184/194 of query aligns to 77:183/186 of 4isxA

query
sites
4isxA

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binding acetyl coenzyme *a: N84 (≠ G86), A104 (≠ G103), W138 (= W141), G140 (= G143), G141 (≠ N144), A159 (= A160), V174 (≠ A175), K182 (≠ G183)

4hurA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with acetyl coenzyme a (see paper)
31% identity, 56% coverage: 78:185/194 of query aligns to 55:166/211 of 4hurA

query
sites
4hurA

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binding acetyl coenzyme *a: S67 (≠ N90), I68 (= I91), G69 (= G92), A79 (vs. gap), N80 (≠ T104), K110 (≠ R125), W120 (= W141), I121 (= I142), G122 (= G143), R123 (≠ N144), M128 (= M149), A140 (= A159), A141 (= A160), T146 (≠ A165), G156 (≠ A175), P159 (= P178), I163 (≠ L182), R164 (≠ G183)

Sites not aligning to the query:

binding acetyl coenzyme *a: 51

4husA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with virginiamycin m1 (see paper)
31% identity, 56% coverage: 78:185/194 of query aligns to 55:166/212 of 4husA

query
sites
4husA

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Sites not aligning to the query:

binding virginiamycin m1: 17, 36, 38

6x3cA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
31% identity, 56% coverage: 78:185/194 of query aligns to 55:166/207 of 6x3cA

query
sites
6x3cA

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binding (3R,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-en-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione: G78 (vs. gap), N80 (≠ T104), H81 (= H105), H91 (= H115), L92 (≠ F116), M101 (vs. gap), P102 (vs. gap), L104 (≠ D119)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: G69 (= G92), N80 (≠ T104), H81 (= H105), W120 (= W141), G122 (= G143), R123 (≠ N144), M128 (= M149), A140 (= A159), A141 (= A160)

Sites not aligning to the query:

6x3jA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f0224 (46) (see paper)
31% identity, 56% coverage: 78:185/194 of query aligns to 55:166/206 of 6x3jA

query
sites
6x3jA

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binding (2R)-2-[(3S,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-4,12-dimethyl-1,7,22-trioxo-4,7,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,3H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosin-3-yl]propyl isoquinolin-3-ylcarbamate: V55 (= V78), H91 (= H115), L92 (≠ F116), M101 (vs. gap), P102 (vs. gap), L107 (≠ V122)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: G69 (= G92), A79 (vs. gap), H81 (= H105), W120 (= W141), G122 (= G143)

Sites not aligning to the query:

binding (2R)-2-[(3S,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-4,12-dimethyl-1,7,22-trioxo-4,7,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,3H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosin-3-yl]propyl isoquinolin-3-ylcarbamate: 51, 53

6x3cE Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
31% identity, 56% coverage: 78:185/194 of query aligns to 55:166/203 of 6x3cE

query
sites
6x3cE

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binding magnesium ion: N158 (≠ I177), P159 (= P178)
binding (3R,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-en-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione: G78 (vs. gap), N80 (≠ T104), H81 (= H105), H91 (= H115), L92 (≠ F116), M101 (vs. gap), P102 (vs. gap)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: G69 (= G92), H81 (= H105), M83 (= M107), W120 (= W141), G122 (= G143), A140 (= A159), A141 (= A160)

Sites not aligning to the query:

3igjC Crystal structure of maltose o-acetyltransferase complexed with acetyl coenzyme a from bacillus anthracis
32% identity, 42% coverage: 102:182/194 of query aligns to 99:183/188 of 3igjC

query
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3igjC

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binding acetyl coenzyme *a: A106 (≠ T109), Y112 (≠ H115), A114 (≠ F117), H116 (≠ D119), G142 (= G143), N148 (≠ M149), A160 (= A159), S161 (≠ A160), T166 (≠ A165), N178 (≠ I177)

Sites not aligning to the query:

binding acetyl coenzyme *a: 84

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
32% identity, 57% coverage: 79:188/194 of query aligns to 78:188/203 of P07464

query
sites
P07464

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H

V

F

H

D

E

D

-

P

-

D

-

V

-

P

-

I

L

R

R

L

K

Q

N

G

G

G

E

R

M

D

Y

E

S

C

F

-

P

V

I

R

T

I

I

G

G

R

N

D

N

C

V

W

W

I

I

G

G

N
x
S

G

H

A

V

I

V

V

I

M

N

A

P

D

G

V

V

-

T

-

I

G

G

D

D

G

N

C

S

V

V

V

I

A

G

A
|
A

G

G

S

S

V

I

V

V

A
x
T

A
x
K

A

D

L

I

A

P

P

P

Y

N

S

V

I

V

A

A

G

G

I

V

P

P

A

C

R
|
R

V

V

L

I

G
x
R

R

E

R

I

G

N

D

D

R

R

N85 (≠ Y88) binding in other chain; binding in other chain
D93 (vs. gap) binding
H115 (= H113) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ N144) binding in other chain
A160 (= A160) binding in other chain
TK 165:166 (≠ AA 165:166) binding
R180 (= R180) binding
R183 (≠ G183) binding in other chain

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding
71 binding

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
32% identity, 57% coverage: 79:188/194 of query aligns to 77:187/200 of 1krrA

query
sites
1krrA

I

I

G

G

R

R

N

N

V

F

Y

Y

I

-

G

-

A

A

Y
x
N

C

F

N

N

I

L

G

T

H

I

A

V

D

D

-

D

-

Y

-

T

-

V

-

T

I

I

G

G

D

D

D

N

S

V

L

L

L
x
I

G
x
A

T
x
P

H

N

V

V

M

T

V

L

T

S

S

V

G
x
T

K

G

H
|
H

T

P

H

V

F

H

D

E

D

-

P

-

D

-

V

-

P

-

I

L

R

R

L

K

Q

N

G

G

G

E

R

M

D

Y

E

S

C

F

-

P

V

I

R

T

I

I

G

G

R

N

D

N

C

V

W

W

I

I

G
|
G

N
x
S

G

H

A

V

I

V

V

I

M
x
N

A

P

D

G

V

V

-

T

-

I

G

G

D

D

G

N

C

S

V

V

V

I

A
x
G

A
|
A

G

G

S

S

V

I

V

V

A

T

A

K

A

D

L

I

A

P

P

P

Y

N

S

V

I

V

A

A

A
|
A

G

G

I

V

P
|
P

A

C

R

R

V

V

L

I

G
x
R

R

E

R

I

G

N

D

D

R

R

binding acetyl coenzyme *a: N84 (≠ Y88), I103 (≠ L102), A104 (≠ G103), P105 (≠ T104), T112 (≠ G111), H114 (= H113), G140 (= G143), S141 (≠ N144), N146 (≠ M149), G158 (≠ A159), A159 (= A160), A174 (= A175), P177 (= P178), R182 (≠ G183)

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
32% identity, 57% coverage: 79:188/194 of query aligns to 77:187/201 of 1krvA

query
sites
1krvA

I

I

G

G

R

R

N

N

V

F

Y
|
Y

I

-

G

-

A

A

Y
x
N

C

F

N

N

I

L

G

T

H

I

A
x
V

D

D

-
x
D

-

Y

-

T

-

V

-

T

I

I

G

G

D

D

D

N

S

V

L

L

L

I

G
x
A

T

P

H

N

V

V

M

T

V

L

T
x
S

S

V

G
x
T

K

G

H

H

T

P

H

V

F

H

D

E

D

-

P

-

D

-

V

-

P

-

I

L

R

R

L

K

Q

N

G

G

G

E

R
x
M

D

Y

E

S

-

F

C

P

V

I

R

T

I

I

G

G

R

N

D

N

C

V

W
|
W

I

I

G
|
G

N
x
S

G

H

A

V

I

V

V

I

M
x
N

A

P

D

G

V

V

-

T

-

I

G

G

D

D

G

N

C

S

V

V

V

I

A

G

A
|
A

G

G

S

S

V

I

V

V

A

T

A

K

A

D

L

I

A

P

P

P

Y

N

S

V

I

V

A

A

G

G

I

V

P
|
P

A

C

R
|
R

V

V

L

I

G
x
R

R

E

R

I

G

N

D

D

R

R

binding 4-nitrophenyl beta-D-galactopyranoside: Y82 (= Y84), N84 (≠ Y88), V90 (≠ A94), D92 (vs. gap), M126 (≠ R130)
binding coenzyme a: A104 (≠ G103), S110 (≠ T109), T112 (≠ G111), W138 (= W141), G140 (= G143), S141 (≠ N144), N146 (≠ M149), A159 (= A160), P177 (= P178), R179 (= R180), R182 (≠ G183)

Sites not aligning to the query:

binding 4-nitrophenyl beta-D-galactopyranoside: 16, 25, 70

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
32% identity, 57% coverage: 79:188/194 of query aligns to 77:187/201 of 1kruA

query
sites
1kruA

I

I

G

G

R

R

N

N

V

F

Y
|
Y

I

-

G

-

A

A

Y
x
N

C

F

N

N

I

L

G

T

H

I

A
x
V

D

D

-
x
D

-

Y

-

T

-

V

-

T

I

I

G

G

D

D

D

N

S

V

L
|
L

L

I

G

A

T

P

H

N

V

V

M

T

V

L

T

S

S

V

G

T

K

G

H
|
H

T

P

H

V

F

H

D

E

D

-

P

-

D

-

V

-

P

-

I

L

R

R

L

K

Q

N

G

G

G

E

R
x
M

D

Y

E

S

-

F

C

P

V

I

R

T

I

I

G

G

R

N

D

N

C

V

W
|
W

I

I

G
|
G

N
x
S

G

H

A

V

I

V

V

I

M

N

A

P

D

G

V

V

-

T

-

I

G

G

D

D

G

N

C

S

V

V

V

I

A
x
G

A
|
A

G

G

S

S

V

I

V

V

A

T

A

K

A

D

L

I

A

P

P

P

Y

N

S

V

I

V

A

A

G

G

I

V

P
|
P

A

C

R

R

V

V

L

I

G
x
R

R

E

R

I

G

N

D

D

R

R

binding coenzyme a: H114 (= H113), W138 (= W141), G140 (= G143), S141 (≠ N144), G158 (≠ A159), A159 (= A160), P177 (= P178), R182 (≠ G183)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: Y82 (= Y84), Y82 (= Y84), N84 (≠ Y88), V90 (≠ A94), D92 (vs. gap), L102 (= L101), H114 (= H113), M126 (≠ R130)

Sites not aligning to the query:

binding 1-methylethyl 1-thio-beta-D-galactopyranoside: 16, 22, 25, 62, 70

6u9cA The 2.2 a crystal structure of the type b chloramphenicol acetyltransferase from vibrio cholerae in the complex with acetyl coa
29% identity, 52% coverage: 85:185/194 of query aligns to 58:162/206 of 6u9cA

query
sites
6u9cA

I

I

G

G

A

S

Y

F

C

C

N

S

I

I

G

G

H

S

A

G

D

-

I

-

G

-

D

-

S

-

L

-

G

-

T

-

H

A

V

V

M

F

V

M

T

M

S

A

G

G

K

N

H

Q

T

G

H

H

-

R

-

S

-

D

-

W

-

I

-

S

-

T

-

F

-

P

-

F

F

F

F

Y

D

Q

D

D

P

N

D

D

V

-

P

-

I

-

R

N

L

F

Q

A

G

D

G

A

R

R

D

D

E

G

C

F

V

T

R

R

-

S

-

G

-

D

-

T

-

I

I

I

G

G

R

H

D

D

C

V

W
|
W

I

I

G

G

N

T

G

E

A

A

I

M

V

I

M

M

A

P

-

G

-

V

D

K

V

I

G

G

D

H

G

G

C

A

V

I

A
|
A

A

S

G

R

S

S

V

V

A

T

A

K

A

D

L

V

A

A

P

P

Y

Y

S

E

I

V

A

V

A

G

G

S

I

N

P

P

A

A

R

K

V

H

L
x
I

G

K

R

F

R

R

binding acetyl coenzyme *a: W116 (= W141), A136 (= A159), I159 (≠ L182)

6pubA Crystal structure of the type b chloramphenicol acetyltransferase from vibrio cholerae in the complex with crystal violet
29% identity, 52% coverage: 85:185/194 of query aligns to 61:165/210 of 6pubA

query
sites
6pubA

I

I

G

G

A

S

Y

F

C

C

N

S

I

I

G

G

H

S

A

G

D

-

I

-

G

-

D

-

S

-

L

-

G

-

T

-

H

A

V

V

M

F

V

M

T

M

S

A

G

G

K

N

H

Q

T

G

H

H

-

R

-

S

-

D

-

W

-

I

-

S

-

T

-

F

-

P

-

F

F

F

F

Y

D

Q

D

D

P

N

D

D

V

-

P

-

I

-

R

N

L

F

Q

A

G

D

G

A

R

R

D

D

E

G

C

F

V

T

R

R

-

S

-

G

-

D

-

T

-

I

I

I

G

G

R

H

D

D

C

V

W

W

I

I

G

G

N

T

G

E

A

A

I

M

V

I

M

M

A

P

-

G

-

V

D

K

V

I

G

G

D

H

G

G

C

A

V

I

A

A

A

S

G

R

S

S

V

V

A

T

A

K

A

D

L

V

A

A

P

P

Y

Y

S

E

I

V

A

V

A

G

G

S

I

N

P

P

A

A

R

K

V

H

L

I

G

K

R

F

R

R

Sites not aligning to the query:

binding crystal violet: 4, 7, 9, 10, 11, 36, 37

8i04A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with serine (see paper)
37% identity, 34% coverage: 125:189/194 of query aligns to 183:247/258 of 8i04A

query
sites
8i04A

R

K

L

T

Q

S

G

G

G

D

R
|
R

D
x
H

E

P

C

K

V

I

R

R

I

E

G

G

R

-

D

-

C

V

W

M

I

I

G

G

N

A

G

G

A

A

I

K

V

I

M

L

A

G

-

N

-

I

D

E

V

V

G

G

D

R

G

G

C

A

V

K

V

I

A

G

A

A

G

G

S

S

V

V

A

L

A

Q

A

P

L

V

A

P

P

P

Y

H

S

T

I

T

A

A

G

G

I

V

P

P

A

A

R

R

V

I

L

V

G

G

R

K

R

P

G

G

D

S

R

D

R

K

binding serine: R188 (= R130), H189 (≠ D131)

Sites not aligning to the query:

binding serine: 88, 89, 153, 154, 180

7uumA Crystal structure of aminoglycoside resistance enzyme apma, complex with paromomycin and coenzyme a (see paper)
34% identity, 28% coverage: 132:185/194 of query aligns to 177:234/274 of 7uumA

query
sites
7uumA

E

E

C

P

V

M

R

T

I

I

G

G

R

S

D

D

C

V

W

Y

I

I

G

G

N

A

G

H

A

A

I
x
F

V

I

M

N

A

A

D

S

-

T

-

V

-

T

-

S

V

I

G

G

D

D

G

G

C

A

V

I

A

G

A

S

G

G

S

A

V

V

A

L

A

E

A

N

L

V

A

P

P

P

Y

F

S

A

I

V

A

V

G

G

I

V

P
|
P

A

A

R

R

V

I

L

K

G

R

R

Y

R

R

binding coenzyme a: F192 (≠ I147), P227 (= P178)

Sites not aligning to the query:

binding coenzyme a: 134, 136, 169
binding paromomycin: 114, 115, 137, 146, 147

Query Sequence

>8499909 FitnessBrowser__Miya:8499909
MLKAFVLVCAVPFYVLHRLEALVLGRERSFAGMTQLLALLPGVYGVLLRVAFLRLALHGC
TSACVVEFMTTFVTPQTVIGRNVYIGAYCNIGHADIGDDSLLGTHVMVTSGKHTHFFDDP
DVPIRLQGGRDECVRIGRDCWIGNGAIVMADVGDGCVVAAGSVVAAALAPYSIAAGIPAR
VLGRRGDRREREEA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory