SitesBLAST
Comparing 8500135 FitnessBrowser__Miya:8500135 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P16171 Mercuric reductase; Hg(II) reductase; EC 1.16.1.1 from Bacillus cereus (see paper)
32% identity, 93% coverage: 6:477/508 of query aligns to 167:610/631 of P16171
- Y264 (≠ S105) mutation to F: 30-fold decrease in activity. 300-fold decrease in activity; when associated with F-605.
- Y605 (= Y472) mutation to F: 10-fold decrease in activity. 300-fold decrease in activity; when associated with F-264.; mutation to H: 2-fold decrease in activity.
2eq7A Crystal structure of lipoamide dehydrogenase from thermus thermophilus hb8 with psbdo
35% identity, 93% coverage: 10:483/508 of query aligns to 3:445/452 of 2eq7A
- active site: P11 (≠ A18), L36 (= L43), C40 (= C47), C45 (= C52), S48 (= S55), G72 (≠ E80), V73 (≠ L81), V177 (≠ P185), E181 (= E189), S314 (≠ G351), H432 (= H470), H434 (≠ Y472), E439 (= E477)
- binding flavin-adenine dinucleotide: G10 (= G17), P11 (≠ A18), G12 (≠ A19), E31 (= E38), K32 (≠ R39), G38 (= G45), T39 (≠ D46), C40 (= C47), R42 (≠ H49), G44 (= G51), C45 (= C52), K49 (= K56), T110 (≠ P120), A111 (= A121), T137 (= T145), G138 (= G146), S157 (≠ N165), I178 (≠ M186), R262 (= R299), Y265 (≠ E302), D302 (= D339), M308 (≠ Q345), L309 (≠ F346), A310 (≠ T347), H311 (= H348), Y341 (≠ F379)
- binding nicotinamide-adenine-dinucleotide: W146 (≠ L154), G174 (= G182), G176 (= G184), V177 (≠ P185), I178 (≠ M186), E197 (≠ L205), Y198 (≠ R206), V231 (= V245), V260 (≠ L297), G261 (= G298), R262 (= R299), M308 (≠ Q345), L309 (≠ F346), V339 (≠ C377)
2yquB Crystal structures and evolutionary relationship of two different lipoamide dehydrogenase(e3s) from thermus thermophilus
35% identity, 93% coverage: 10:483/508 of query aligns to 3:445/455 of 2yquB
- active site: P11 (≠ A18), L36 (= L43), C40 (= C47), C45 (= C52), S48 (= S55), G72 (≠ E80), V73 (≠ L81), V177 (≠ P185), E181 (= E189), S314 (≠ G351), H432 (= H470), H434 (≠ Y472), E439 (= E477)
- binding carbonate ion: A310 (≠ T347), S314 (≠ G351), S423 (≠ R461), D426 (≠ A464)
- binding flavin-adenine dinucleotide: G8 (= G15), G10 (= G17), P11 (≠ A18), G12 (≠ A19), E31 (= E38), K32 (≠ R39), G38 (= G45), T39 (≠ D46), C40 (= C47), R42 (≠ H49), G44 (= G51), C45 (= C52), K49 (= K56), T110 (≠ P120), A111 (= A121), T137 (= T145), G138 (= G146), I178 (≠ M186), Y265 (≠ E302), G301 (= G338), D302 (= D339), M308 (≠ Q345), L309 (≠ F346), A310 (≠ T347), H311 (= H348)
2yquA Crystal structures and evolutionary relationship of two different lipoamide dehydrogenase(e3s) from thermus thermophilus
35% identity, 93% coverage: 10:483/508 of query aligns to 3:445/455 of 2yquA
- active site: P11 (≠ A18), L36 (= L43), C40 (= C47), C45 (= C52), S48 (= S55), G72 (≠ E80), V73 (≠ L81), V177 (≠ P185), E181 (= E189), S314 (≠ G351), H432 (= H470), H434 (≠ Y472), E439 (= E477)
- binding flavin-adenine dinucleotide: G8 (= G15), G10 (= G17), P11 (≠ A18), G12 (≠ A19), E31 (= E38), K32 (≠ R39), G38 (= G45), T39 (≠ D46), C40 (= C47), R42 (≠ H49), G44 (= G51), C45 (= C52), K49 (= K56), T110 (≠ P120), A111 (= A121), T137 (= T145), G138 (= G146), S157 (≠ N165), I178 (≠ M186), Y265 (≠ E302), G301 (= G338), D302 (= D339), M308 (≠ Q345), L309 (≠ F346), A310 (≠ T347)
4k8dA Crystal structure of the c558(464)a/c559(465)a double mutant of tn501 mera in complex with NADPH and hg2+
34% identity, 92% coverage: 13:477/508 of query aligns to 8:445/466 of 4k8dA
- active site: G13 (≠ A18), I37 (≠ L43), C41 (= C47), C46 (= C52), S49 (= S55), V75 (≠ L81), P76 (≠ E82), V185 (≠ P185), E189 (= E189), A320 (≠ G351), F438 (≠ H470), Y440 (= Y472), E445 (= E477)
- binding flavin-adenine dinucleotide: I9 (= I14), G10 (= G15), G12 (= G17), A14 (= A19), E33 (= E38), R34 (= R39), G39 (= G45), T40 (≠ D46), C41 (= C47), G45 (= G51), C46 (= C52), K50 (= K56), E115 (≠ P120), A116 (= A121), T145 (= T145), G146 (= G146), R268 (= R299), G307 (= G338), D308 (= D339), F315 (= F346), V316 (≠ T347), Y317 (≠ H348)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: S183 (≠ G183), S184 (≠ G184), V185 (≠ P185), V186 (≠ M186), E189 (= E189), R206 (= R206), N207 (≠ G207), R212 (= R213), T266 (≠ L297), G267 (= G298), Q314 (= Q345), F315 (= F346), V345 (≠ C377)
Sites not aligning to the query:
P00392 Mercuric reductase; Hg(II) reductase; EC 1.16.1.1 from Pseudomonas aeruginosa (see 2 papers)
34% identity, 94% coverage: 1:477/508 of query aligns to 86:540/561 of P00392
- A110 (≠ G20) binding
- G130 (≠ P41) binding
- T135 (≠ D46) binding
- C136 (= C47) modified: Disulfide link with 141, Redox-active
- C141 (= C52) modified: Disulfide link with 136, Redox-active
- K145 (= K56) binding
- A211 (= A121) binding
- D403 (= D339) binding
- V411 (≠ T347) binding
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 558 binding
- 559 binding
P11959 Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; EC 1.8.1.4 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
32% identity, 94% coverage: 8:483/508 of query aligns to 9:457/470 of P11959
- 39:47 (vs. 38:47, 50% identical) binding
- K56 (= K56) binding
- D314 (= D339) binding
- A322 (≠ T347) binding
1ebdA Dihydrolipoamide dehydrogenase complexed with the binding domain of the dihydrolipoamide acetylase (see paper)
32% identity, 94% coverage: 8:483/508 of query aligns to 3:451/455 of 1ebdA
- active site: P13 (≠ A18), L37 (= L43), C41 (= C47), C46 (= C52), S49 (= S55), N74 (≠ E80), V75 (≠ L81), Y180 (≠ P185), E184 (= E189), S320 (≠ G351), H438 (= H470), H440 (≠ Y472), E445 (= E477)
- binding flavin-adenine dinucleotide: G10 (= G15), G12 (= G17), P13 (≠ A18), V32 (≠ A37), E33 (= E38), K34 (≠ R39), G39 (= G45), V40 (≠ D46), C41 (= C47), G45 (= G51), C46 (= C52), K50 (= K56), E112 (≠ P120), A113 (= A121), T141 (= T145), G142 (= G146), Y180 (≠ P185), I181 (≠ M186), R268 (= R299), D308 (= D339), A314 (≠ Q345), L315 (≠ F346), A316 (≠ T347)
4k7zA Crystal structure of the c136(42)a/c141(47)a double mutant of tn501 mera in complex with NADP and hg2+
34% identity, 92% coverage: 13:477/508 of query aligns to 9:446/467 of 4k7zA
- active site: G14 (≠ A18), I38 (≠ L43), A42 (≠ C47), A47 (≠ C52), S50 (= S55), V76 (≠ L81), P77 (≠ E82), V186 (≠ P185), E190 (= E189), A321 (≠ G351), F439 (≠ H470), Y441 (= Y472), E446 (= E477)
- binding flavin-adenine dinucleotide: I10 (= I14), G11 (= G15), G13 (= G17), A15 (= A19), E34 (= E38), R35 (= R39), G40 (= G45), T41 (≠ D46), A42 (≠ C47), G46 (= G51), A47 (≠ C52), K51 (= K56), E116 (≠ P120), A117 (= A121), T146 (= T145), G147 (= G146), R269 (= R299), G308 (= G338), D309 (= D339), Q315 (= Q345), F316 (= F346), V317 (≠ T347), Y318 (≠ H348)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: S184 (≠ G183), S185 (≠ G184), V186 (≠ P185), V187 (≠ M186), E190 (= E189), R207 (= R206), N208 (≠ G207), R213 (= R213), T267 (≠ L297), G268 (= G298), R269 (= R299), Q315 (= Q345), F316 (= F346), V346 (≠ C377)
Sites not aligning to the query:
4ywoA Mercuric reductase from metallosphaera sedula (see paper)
32% identity, 94% coverage: 2:477/508 of query aligns to 1:432/444 of 4ywoA
- active site: A15 (= A18), I39 (≠ L43), C43 (= C47), C48 (= C52), S51 (= S55), A174 (≠ P185), E178 (= E189), G308 (= G351), H425 (= H470), F427 (≠ Y472), E432 (= E477)
- binding flavin-adenine dinucleotide: G12 (= G15), G14 (= G17), K36 (≠ R39), G41 (= G45), T42 (≠ D46), C43 (= C47), G47 (= G51), C48 (= C52), K52 (= K56), A110 (= A121), A133 (= A144), T134 (= T145), G135 (= G146), N154 (= N165), L175 (≠ M186), L263 (= L306), G295 (= G338), D296 (= D339), M302 (≠ Q345), L303 (≠ F346), E304 (≠ T347), A307 (= A350)
3o0hB Crystal structure of glutathione reductase from bartonella henselae
30% identity, 93% coverage: 8:477/508 of query aligns to 3:440/459 of 3o0hB
- active site: S13 (≠ A18), I37 (≠ L43), C41 (= C47), C46 (= C52), K49 (≠ S55), D74 (vs. gap), P75 (= P77), Y177 (≠ P185), E181 (= E189), I314 (≠ G351), A433 (≠ H470), H435 (≠ Y472), E440 (= E477)
- binding flavin-adenine dinucleotide: I9 (= I14), G10 (= G15), G12 (= G17), S13 (≠ A18), G14 (≠ A19), A32 (= A37), E33 (= E38), E34 (= E40), T40 (≠ D46), C41 (= C47), G45 (= G51), C46 (= C52), K49 (≠ S55), R112 (≠ P120), A113 (= A121), T139 (= T145), G140 (= G146), Y177 (≠ P185), R262 (= R299), N265 (≠ E302), G301 (= G338), D302 (= D339), Q308 (= Q345), L309 (≠ F346), T310 (= T347)
Sites not aligning to the query:
2eq6A Crystal structure of lipoamide dehydrogenase from thermus thermophilus hb8
36% identity, 93% coverage: 10:483/508 of query aligns to 5:448/460 of 2eq6A
- active site: V37 (≠ L43), C41 (= C47), C46 (= C52), T49 (≠ S55), A176 (≠ P185), E180 (= E189), H435 (= H470), H437 (≠ Y472), E442 (= E477)
- binding flavin-adenine dinucleotide: I9 (= I14), G10 (= G15), G12 (= G17), P13 (≠ A18), G14 (≠ A19), E33 (≠ R39), A34 (≠ E40), G39 (= G45), V40 (≠ D46), C41 (= C47), G45 (= G51), C46 (= C52), K50 (= K56), F111 (≠ P120), A112 (= A121), A135 (= A144), T136 (= T145), G137 (= G146), S155 (≠ T164), R269 (≠ E302), D306 (= D339), L312 (≠ Q345), L313 (≠ F346), A314 (≠ T347), H315 (= H348), Y344 (≠ F379)
Sites not aligning to the query:
1dxlA Dihydrolipoamide dehydrogenase of glycine decarboxylase from pisum sativum (see paper)
31% identity, 94% coverage: 8:483/508 of query aligns to 3:457/467 of 1dxlA
- active site: L38 (= L43), C42 (= C47), C47 (= C52), S50 (= S55), Y184 (≠ P185), E188 (= E189), H444 (= H470), H446 (≠ Y472), E451 (= E477)
- binding flavin-adenine dinucleotide: I9 (= I14), P13 (≠ A18), G14 (≠ A19), E33 (= E38), K34 (≠ R39), R35 (≠ E40), G40 (= G45), T41 (≠ D46), C42 (= C47), G46 (= G51), C47 (= C52), K51 (= K56), Y114 (≠ L112), G115 (= G113), T144 (= T145), G145 (= G146), Y184 (≠ P185), I185 (≠ M186), R274 (= R299), D314 (= D339), M320 (≠ Q345), L321 (≠ F346), A322 (≠ T347), H323 (= H348)
4jdrA Dihydrolipoamide dehydrogenase of pyruvate dehydrogenase from escherichia coli (see paper)
31% identity, 92% coverage: 11:477/508 of query aligns to 8:449/471 of 4jdrA
- active site: P15 (≠ A18), L40 (= L43), C44 (= C47), C49 (= C52), S52 (= S55), E77 (≠ L76), P78 (= P77), I184 (≠ P185), E188 (= E189), V324 (≠ G351), H442 (= H470), H444 (≠ Y472), E449 (= E477)
- binding flavin-adenine dinucleotide: G12 (= G15), G14 (= G17), P15 (≠ A18), A16 (= A19), E35 (= E38), R36 (= R39), Y37 (≠ E40), V43 (≠ D46), C44 (= C47), G48 (= G51), C49 (= C52), K53 (= K56), L115 (≠ P120), G116 (≠ A121), A144 (≠ T145), G145 (= G146), I185 (≠ M186), G311 (= G338), D312 (= D339), M318 (≠ Q345), L319 (≠ F346), A320 (≠ T347), H321 (= H348)
Sites not aligning to the query:
P31023 Dihydrolipoyl dehydrogenase, mitochondrial; Dihydrolipoamide dehydrogenase; Glycine cleavage system L protein; Pyruvate dehydrogenase complex E3 subunit; E3; PDC-E3; EC 1.8.1.4 from Pisum sativum (Garden pea) (Lathyrus oleraceus) (see 2 papers)
31% identity, 94% coverage: 8:483/508 of query aligns to 37:491/501 of P31023
- 67:76 (vs. 38:47, 60% identical) binding
- C76 (= C47) modified: Disulfide link with 81, Redox-active
- C81 (= C52) modified: Disulfide link with 76, Redox-active
- G149 (= G113) binding
- D348 (= D339) binding
- MLAH 354:357 (≠ QFTH 345:348) binding
Sites not aligning to the query:
- 1:31 modified: transit peptide, Mitochondrion
P0A9P0 Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein; EC 1.8.1.4 from Escherichia coli (strain K12) (see 2 papers)
31% identity, 92% coverage: 11:477/508 of query aligns to 9:450/474 of P0A9P0
- K220 (≠ G220) modified: N6-acetyllysine
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
P09063 Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; E3 component of branched-chain alpha-keto acid dehydrogenase complex; LPD-Val; EC 1.8.1.4 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see paper)
33% identity, 93% coverage: 11:483/508 of query aligns to 9:449/459 of P09063
- 36:44 (vs. 38:47, 60% identical) binding
- C44 (= C47) modified: Disulfide link with 49, Redox-active
- C49 (= C52) modified: Disulfide link with 44, Redox-active
- K53 (= K56) binding
- A119 (= A121) binding
- A142 (= A144) binding
- GGGYI 179:183 (≠ GGGPM 182:186) binding
- Y182 (≠ P185) binding
- E202 (≠ L205) binding
- V236 (= V239) binding
- AVGR 264:267 (≠ ALGR 296:299) binding
- D306 (= D339) binding
- M312 (≠ Q345) binding
- A314 (≠ T347) binding
1lvlA The refined structure of pseudomonas putida lipoamide dehydrogenase complexed with NAD+ at 2.45 angstroms resolution (see paper)
33% identity, 93% coverage: 11:483/508 of query aligns to 8:448/458 of 1lvlA
- active site: P15 (≠ A18), L39 (= L43), C43 (= C47), C48 (= C52), S51 (= S55), S79 (≠ A90), P80 (≠ R91), Y181 (≠ P185), E185 (= E189), M317 (≠ G351), H435 (= H470), H437 (≠ Y472), E442 (= E477)
- binding flavin-adenine dinucleotide: I11 (= I14), G12 (= G15), E35 (= E38), G36 (≠ R39), G41 (= G45), T42 (≠ D46), C43 (= C47), G47 (= G51), C48 (= C52), K52 (= K56), W117 (≠ P120), A118 (= A121), A141 (= A144), T142 (= T145), G143 (= G146), Y181 (≠ P185), R266 (= R299), R269 (≠ E302), D305 (= D339), L312 (≠ F346), A313 (≠ T347), H314 (= H348)
- binding nicotinamide-adenine-dinucleotide: V177 (≠ L181), G178 (= G182), Y181 (≠ P185), I182 (≠ M186), E201 (≠ L205), A263 (= A296), V264 (≠ L297), G265 (= G298), R266 (= R299), E309 (≠ D343), M311 (≠ Q345)
4dnaA Crystal structure of putative glutathione reductase from sinorhizobium meliloti 1021
33% identity, 87% coverage: 8:448/508 of query aligns to 3:413/461 of 4dnaA
- active site: Y37 (≠ L43), C41 (= C47), C46 (= C52), K49 (≠ S55), Y178 (≠ P185), E182 (= E189)
- binding flavin-adenine dinucleotide: I9 (= I14), G10 (= G15), G11 (= G16), G12 (= G17), G14 (≠ A19), E33 (= E38), E34 (= E40), G39 (= G45), T40 (≠ D46), C41 (= C47), G45 (= G51), C46 (= C52), K49 (≠ S55), R112 (≠ P120), A113 (= A121), V139 (≠ T145), G140 (= G146), Y178 (≠ P185), R264 (= R299), G303 (= G338), D304 (= D339), Q310 (= Q345), L311 (≠ F346), T312 (= T347)
Sites not aligning to the query:
2rabA Structure of glutathione amide reductase from chromatium gracile in complex with NAD (see paper)
32% identity, 91% coverage: 10:471/508 of query aligns to 5:434/451 of 2rabA
- active site: S13 (≠ A18), L37 (= L43), C41 (= C47), C46 (= C52), K49 (≠ S55), Y173 (≠ P185), E177 (= E189), I310 (≠ H352), A431 (= A468), H433 (= H470)
- binding flavin-adenine dinucleotide: G10 (= G15), G12 (= G17), S13 (≠ A18), G14 (≠ A19), I32 (≠ A37), E33 (= E38), S34 (≠ R39), T40 (≠ D46), G45 (= G51), C46 (= C52), K49 (≠ S55), H110 (≠ P120), A111 (= A121), T135 (= T145), G136 (= G146), R258 (= R299), G297 (= G338), D298 (= D339), Q304 (= Q345), L305 (≠ F346), T306 (= T347)
- binding nicotinamide-adenine-dinucleotide: K49 (≠ S55), I169 (≠ L181), G172 (= G184), Y173 (≠ P185), I174 (≠ M186), E177 (= E189), A193 (≠ L205), L194 (≠ R206), E195 (≠ G207), V227 (= V245), V256 (≠ L297), G257 (= G298), Q304 (= Q345), V337 (≠ C377)
Sites not aligning to the query:
Query Sequence
>8500135 FitnessBrowser__Miya:8500135
MARTPRHDADLLVIGGGAAGLTAAAGAARFGARVVLAEREPALGGDCLHHGCVPSKTLLA
TARARHVMRRAALFGLPAPELEPVDFAAVARRIREVQAVIQRHDSPQRFTGLGVDVRFGP
ARFCDEHTVEIGGRRVSAARILIATGSSPQLPPLPGLDTVPFLTNRDIFSLDALPASLLV
LGGGPMACEMAQAFARLGSRVTMVLRGPRILPRDDADMAGVVHASLAADGVRVLAGATVK
MLRAVSAISGRFGKPGEPVGADGNDAAGTGVEAELEVPQGEGRGPLVVRADRLLAALGRT
PETAGLDLAAAGVATGRHGGITVDGRMRTSQPHVFAAGDVTGDWQFTHAAGHEAGVVVAN
AVLRLPRRADHARMPWCTFTDPELASVGCNERMAAERGLPVDVHVEPFAANDRALAEGTP
EGRLKLVLRKGGNRVLGVQAVGPHAGEVLNEWVAVLGGGVRLSALAGAVHPYPTLGEISA
RAAGNVVSRVLFSVGARRLLRLLFGYRG
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory