SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 8500263 DvMF_1020 oligopeptide/dipeptide ABC transporter, ATPase subunit (RefSeq) to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0AAH4 Putrescine export system ATP-binding protein SapD from Escherichia coli (strain K12) (see paper)
39% identity, 85% coverage: 17:320/358 of query aligns to 3:305/330 of P0AAH4

query
sites
P0AAH4

L

L

D

D

V

I

R

R

D

N

L

L

S

T

V

I

E

E

F

F

P

K

G

-

R

-

P

-

E

-

A

-

D

-

G

-

T

T

P

G

G

D

A

E

P

W

L

V

R

K

A

A

V

V

A

D

G

R

I

V

S

S

F

M

S

T

I

L

A

T

P

E

G

G

G

E

T

I

L

R

C

G

L

L

V

V

G

G

E

E

S

S

G

G

C

S

G

G

K
|
K

S

S

M

L

T

I

A

A

L

K

A

A

L

I

L

C

R

G

L

V

V

N

P

K

E

D

P

N

G

W

R

R

V

V

A

T

S

A

G

D

S

R

V

M

R

R

L

F

E

D

G

D

R

I

E

D

L

L

T

R

L

L

P

S

E

A

P

R

E

E

M

R

R

R

G

K

V

L

R

V

G

G

R

H

S

N

I

V

S

S

M

M

I

I

F

F

Q

Q

E

E

P

P

M

Q

T

S

S

C

L

L

N

D

P

P

V

S

F

E

R

R

V

V

G

G

E

R

Q

Q

I

L

A

M

E

Q

G

N

V

I

R

P

L

A

H

W

L

T

G

Y

L

K

S

G

R

R

S

W

D

W

A

Q

-

R

-

F

-

G

-

W

-

R

A

K

A

R

R

R

A

A

V

I

D

E

L

L

R

H

Q

R

V

V

G

G

I

I

P

K

S

D

P

H

E

K

L

D

R

A

A

M

R

R

D

S

F

F

P

P

H

Y

Q

E

M

L

S

T

G

E

G
|
G

M

E

R

C

Q
|
Q

R

K

V

V

M

M

I

I

A

A

M

I

A

A

L

L

A

A

C

N

D

Q

P

P

R

R

L

L

I

I

A

A

D
|
D

E

E

P

P

T

T

T

N

A

S

L

M

D

E

V

P

T

T

I

T

Q

Q

R

A

Q

Q

I

I

L

F

R

R

L

L

M

L

R

T

D

R

L

L

A

N

A

Q

H

N

R

S

G

N

A

T

S

T

V

I

L

L

I

I

T

S

H

H

D

D

L

L

G

Q

V

M

V

L

A

S

E

Q

T

W

A

A

D

D

H

K

V

I

A

N

V

V

M

L

Y

Y

A

C

G

G

R

Q

I

T

M

V

E

E

H

T

A

A

S

P

V

S

G

K

D

E

F

L

F

V

A

T

R

M

P

P

L

H

H

H

P

P

Y

Y

A

T

Q

Q

G

A

L

L

M

I

R

R

S

A

V

I

P

P

Q

D

A

F

V

G

S

S

G

A

-

M

P

P

R

H

A

K

E

S

R

R

L

L

S

N

A

T

I

L

P

P

G

G

T

A

V

I

P

P

P

L

L

L

W

E

A

Q

L

L

P

P

S

I

G

G

C

C

T

R

F

L

R

G

D

P

R

R

C

C

P

P

H

Y

A

A

F

Q

A

R

R

E

C

C

K46 (= K67) mutation K->G,I,Q,R: 25% to 50% stimulation of K(+) uptake by TrkH.; mutation Missing: Almost no stimulation of K(+) uptake by TrkH.
G162 (= G178) mutation to A: 80% stimulation of K(+) uptake by TrkH.
Q165 (= Q181) mutation to L: 60% stimulation of K(+) uptake by TrkH.
D183 (= D199) mutation to E: About 60% stimulation of K(+) uptake by TrkH.; mutation D->K,N: 15% to 20% stimulation of K(+) uptake by TrkH.

Q8RDH4 Dipeptide transport ATP-binding protein DppD; EC 7.4.2.9 from Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis) (see paper)
39% identity, 83% coverage: 41:337/358 of query aligns to 21:315/326 of Q8RDH4

query
sites
Q8RDH4

L

I

R

K

A

A

V

A

A

D

G

G

I

I

S

S

F

L

S

D

I

I

A

L

P

E

G

N

G

S

T

V

L

T

C

A

L

I

V

V

G

G

E

E

S

S

G
x
A

C
x
S

G
|
G

K
|
K

S
|
S

M
x
T

T

I

A

I

L

E

A

A

L

M

L

T

R

K

L

T

V

L

P

P

E

P

P
x
N

G

G

R

R

V

I

A

L

S

S

G

G

S

R

V

V

R

L

L

Y

E

K

G

G

R

K

E

D

L

L

T

T

L

M

P

R

E

E

P

E

E

E

M

L

R

R

G

K

V

I

R

R

G

W

R

K

S

E

I

I

S

A

M

L

I

V

F

P

Q
|
Q

E

A

P

A

M

Q

T

Q

S

S

L

L

N

N

P

P

V

T

F

M

R

K

V

V

G

I

E

E

Q

H

I

F

A

K

E

D

G

T

V

V

R

E

L

A

H

H

-

G

L

V

G

R

L

W

S

S

R

H

S

S

D

E

A

L

A

I

A

E

R

K

A

A

V

S

D

E

L

K

L

L

R

R

Q

M

V

V

G

R

I

L

P

-

S

N

P

P

E

E

L

A

R

V

A

L

R

N

D

S

F

Y

P

P

H

L

Q

Q

M

L

S

S

G

G

M

M

R

K

Q

Q

R

R

V

V

M

L

I

I

A

A

M

L

A

A

L

L

A

L

C

L

D

D

P

P

R

V

L

V

L

L

I

I

A

L

D

D

E

E

P

P

T

T

T

S

A

A

L

L

D

D

V

V

T

L

I

T

Q

Q

R

A

Q

H

I

I

L

I

R

Q

L

L

M

L

R

K

D

E

L

L

A

K

H

M

R

L

G

K

A

I

S

T

V

L

L

I

L

F

I

V

T

T

H

H

D

D

L

I

G

A

V

V

V

A

A

A

E

E

T

L

A

A

D

D

H

K

V

V

A

A

V

V

M

I

Y

Y

A

G

G

G

R

N

I

L

M

V

E

E

H

Y

A

N

S

S

V

T

G

F

D

Q

F

I

F

F

A

K

R

N

P

P

L

L

H

H

P

P

Y

Y

A

T

Q

R

G

G

L

L

M

I

R

N

S

S

V

I

P

-

Q

M

A

A

V

V

S

N

G

A

P

D

R

M

A

S

E

-

R

K

L

V

S

K

A

P

I

I

P

P

G

G

T

D

V

P

P

P

P

S

L

L

W

L

A

N

L

P

P

P

S

S

G

G

C
|
C

T

R

F

F

R

H

D

P

R

R

C
|
C

P

E

H

Y

A

A

F

M

A

E

R

I

C
|
C

A

K

E

K

Q

E

E

K

P

P

P

K

L

W

L

I

A

R

M

L

P

D

P

G

Q

E

A

A

L

H

A

V

A

A

ASGKST 44:49 (≠ GCGKSM 64:69) binding
N61 (≠ P81) binding
Q97 (= Q117) binding
C285 (= C307) binding
C291 (= C313) binding
C298 (= C320) binding

Sites not aligning to the query:

316 binding

4fwiB Crystal structure of the nucleotide-binding domain of a dipeptide abc transporter (see paper)
39% identity, 83% coverage: 41:337/358 of query aligns to 20:304/310 of 4fwiB

query
sites
4fwiB

L
x
I

R

K

A

A

V

A

A

D

G

G

I

I

S

S

F

L

S

D

I

I

A

L

P

E

G

N

G

S

T

V

L

T

C

A

L

I

V

V

G

G

E

E

S
|
S

G
x
A

C
x
S

G
|
G

K
|
K

S
|
S

M
x
T

T

I

A

I

L

E

A

A

L

M

L

T

R

K

L

T

V

L

P

P

E

P

P
x
N

G

G

R

R

V

I

A

L

S

S

G

G

S

R

V

V

R

L

L

Y

E

K

G

G

R

K

E

D

L

L

T

T

L

M

P

R

E

E

P

E

E

E

M

L

R

R

G

K

V

I

R

R

G

W

R

K

S

E

I

I

S

A

M

L

I

V

F

P

Q
|
Q

E

A

P

A

M

Q

T

Q

S

S

L

L

N

N

P

P

V

T

F

M

R

K

V

V

G

I

E

E

Q

H

I

F

A

K

E

D

G

T

V

V

R

E

L

A

H

H

-

G

L

V

G

R

L

W

S

S

R

H

S

S

D

E

A

L

A

I

A

E

R

K

A

A

V

S

D

E

L

K

L

L

R

R

Q

M

V

V

G

R

I

L

P

-

S

N

P

P

E

E

L

A

R

V

A

L

R

N

D

S

F

Y

P

P

H

L

Q

Q

M

L

S

S

G

G

M

M

R

K

Q

Q

R

R

V

V

M

L

I

I

A

A

M

L

A

A

L

L

A

L

C

L

D

D

P

P

R

V

L

V

L

L

I

I

A

L

D
|
D

E
|
E

P

P

T

T

T

S

A

A

L

L

D

D

V

V

T

L

I

T

Q

Q

R

A

Q

H

I

I

L

I

R

Q

L

L

M

L

R

K

D

E

L

L

A

K

H

M

R

L

G

K

A

I

S

T

V

L

L

I

L

F

I

V

T

T

H

H

D

D

L

I

G

A

V

V

V

A

A

A

E

E

T

L

A

A

D

D

H

K

V

V

A

A

V

V

M

I

Y

Y

A

G

G

G

R

N

I

L

M

V

E

E

H

Y

A

N

S

S

V

T

G

F

D

Q

F

I

F

F

A

K

R

N

P

P

L

L

H
|
H

P
|
P

Y

Y

A

T

Q

R

G

G

L

L

M

I

R

N

S

S

V

I

P

-

Q

-

A

-

V

-

S

-

G

-

P

-

R

-

A

-

E

-

R

-

L

-

S

M

A

P

I

I

P

P

G

G

T

D

V

P

P

P

P

S

L
|
L

W

L

A

N

L

P

P

P

S

S

G

G

C
|
C

T

R

F

F

R
x
H

D

P

R

R

C
|
C

P

E

H
x
Y

A
|
A

F

M

A

E

R

I

C
|
C

A

K

E

K

Q

E

E

K

P
|
P

P

K

L

W

L

I

A

R

M

L

P

D

P

G

Q

E

A

A

L

H

A

V

A

A

binding adenosine-5'-triphosphate: I20 (≠ L41), S42 (= S63), A43 (≠ G64), S44 (≠ C65), G45 (= G66), K46 (= K67), S47 (= S68), T48 (≠ M69), N60 (≠ P81), Q96 (= Q117), E179 (= E200), L267 (= L300)
binding magnesium ion: S47 (= S68), Q96 (= Q117), D178 (= D199)
binding iron/sulfur cluster: H247 (= H268), P248 (= P269), C274 (= C307), H277 (≠ R310), C280 (= C313), Y282 (≠ H315), A283 (= A316), C287 (= C320), P292 (= P325)

Sites not aligning to the query:

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
36% identity, 72% coverage: 41:296/358 of query aligns to 18:263/343 of P30750

query
sites
P30750

L

I

R

Q

A

A

V

L

A

N

G

N

I

V

S

S

F

L

S

H

I

V

A

P

P

A

G

G

G

Q

T

I

L

Y

C

G

L

V

V

I

G

G

E

A

S
|
S

G
|
G

C
x
A

G
|
G

K
|
K

S
|
S

M

-

T

-

A

-

L

-

A
x
T

L

L

L

I

R

R

L

C

V

V

P

N

E

L

P

L

G

E

R

R

V

P

A

T

S

E

G

G

S

S

V

V

R

L

L

V

E

D

G

G

R

Q

E

E

L

L

L

T

T

T

L

L

P

S

E

E

P

S

E

E

M

L

R

T

G

K

V

A

R

R

G

-

R

R

S

Q

I

I

S

G

M

M

I

I

F

F

Q

Q

E

H

-

F

P

N

M

L

T

L

S

S

L

S

N

R

P

T

V

V

F

F

R

G

V

-

G

-

E

-

Q

-

I

-

A

-

E

-

G

N

V

V

R

A

L

L

H

P

L

L

G

E

L

L

S

D

-

N

-

T

-

P

R

K

S

D

D

E

A

V

A

K

A

R

R

R

A

V

V

T

D

E

L

L

R

S

Q

L

V

V

G

G

I

L

P

G

S

D

P

-

E

-

L

-

R

K

A

H

R

D

D

S

F

Y

P

P

H

S

Q

N

M

L

S

S

G

G

M

Q

R

K

Q

Q

R

R

V

V

M

A

I

I

A

A

M

R

A

A

L

L

A

A

C

S

D

N

P

P

R

K

L

V

L

L

I

L

A

C

D

D

E
|
E

P

A

T

T

T

S

A

A

L

L

D

D

V

P

T

A

I

T

Q

T

R

R

Q

S

I

I

L

L

R

E

L

L

M

L

R

K

D

D

L

I

A

N

A

R

H

R

R

L

G

G

A

L

S

T

V

I

L

L

I

I

T

T

H

H

D

E

L

M

G

D

V

V

A

K

E

R

T

I

A

C

D

D

H

C

V

V

A

A

V

V

M

I

Y

S

A

N

G

G

R

E

I

L

M

I

E

E

H

Q

A

D

S

T

V

V

G

S

D

E

F

V

F

F

A

S

R

H

P

P

L

K

H

T

P

P

Y

L

A

A

Q

Q

G

K

L

F

M

I

R

Q

S

S

V

T

P

L

Q

H

A

L

-

D

V

I

S

P

G

E

P

D

R

Y

A

Q

E

E

R

R

L

L

S

Q

A

A

I

E

P

P

G

F

T

T

40:46 (vs. 63:73, 45% identical) binding
E166 (= E200) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
36% identity, 72% coverage: 41:296/358 of query aligns to 19:264/344 of 3tuzC

query
sites
3tuzC

L

I

R

Q

A
|
A

V

L

A

N

G

N

I

V

S

S

F

L

S

H

I

V

A

P

P

A

G

G

G

Q

T

I

L

Y

C

G

L

V

V

I

G

G

E

A

S

S

G
|
G

C
x
A

G
|
G

K
|
K

S
|
S

M

-

T

-

A

-

L

-

A
x
T

L

L

L

I

R

R

L

C

V

V

P

N

E

L

P

L

G

E

R

R

V

P

A

T

S

E

G

G

S

S

V

V

R

L

L

V

E

D

G

G

R

Q

E

E

L

L

L

T

T

T

L

L

P

S

E

E

P

S

E

E

M

L

R

T

G

K

V

A

R

R

G

-

R

R

S

Q

I

I

S

G

M

M

I

I

F

F

Q

Q

E

H

-

F

P

N

M

L

T

L

S

S

L

S

N

R

P

T

V

V

F

F

R

G

V

-

G

-

E

-

Q

-

I

-

A

-

E

-

G

N

V

V

R

A

L

L

H

P

L

L

G

E

L

L

S

D

-

N

-

T

-

P

R

K

S

D

D

E

A

V

A

K

A

R

R

R

A

V

V

T

D

E

L

L

R

S

Q

L

V

V

G

G

I

L

P

G

S

D

P

-

E

-

L

-

R

K

A

H

R

D

D

S

F

Y

P

P

H

S

Q

N

M

L

S

S

G

G

M

Q

R

K

Q

Q

R

R

V

V

M

A

I

I

A

A

M

R

A

A

L

L

A

A

C

S

D

N

P

P

R

K

L

V

L

L

I

L

A

C

D

D

E

Q

P

A

T

T

T

S

A

A

L

L

D

D

V

P

T

A

I

T

Q

T

R

R

Q

S

I

I

L

L

R

E

L

L

M

L

R

K

D

D

L

I

A

N

A

R

H

R

R

L

G

G

A

L

S

T

V

I

L

L

I

I

T

T

H

H

D

E

L

M

G

D

V

V

A

K

E

R

T

I

A

C

D

D

H

C

V

V

A

A

V

V

M

I

Y

S

A

N

G

G

R

E

I

L

M

I

E

E

H

Q

A

D

S

T

V

V

G

S

D

E

F

V

F

F

A

S

R

H

P

P

L

K

H

T

P

P

Y

L

A

A

Q

Q

G

K

L

F

M

I

R

Q

S

S

V

T

P

L

Q

H

A

L

-

D

V

I

S

P

G

E

P

D

R

Y

A

Q

E

E

R

R

L

L

S

Q

A

A

I

E

P

P

G

F

T

T

binding adenosine-5'-diphosphate: A21 (= A43), G42 (= G64), A43 (≠ C65), G44 (= G66), K45 (= K67), S46 (= S68), T47 (≠ A73)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 14
binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 312, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
36% identity, 72% coverage: 41:296/358 of query aligns to 19:264/344 of 3tuiC

query
sites
3tuiC

L

I

R

Q

A

A

V

L

A

N

G

N

I

V

S

S

F

L

S

H

I

V

A

P

P

A

G

G

G

Q

T

I

L

Y

C

G

L

V

V

I

G

G

E

A

S
|
S

G
|
G

C
x
A

G
|
G

K
|
K

S
|
S

M

-

T

-

A

-

L

-

A
x
T

L

L

L

I

R

R

L

C

V

V

P

N

E

L

P

L

G

E

R

R

V

P

A

T

S

E

G

G

S

S

V

V

R

L

L

V

E

D

G

G

R

Q

E

E

L

L

L

T

T

T

L

L

P

S

E

E

P

S

E

E

M

L

R

T

G

K

V

A

R

R

G

-

R

R

S

Q

I

I

S

G

M

M

I

I

F

F

Q

Q

E

H

-

F

P

N

M

L

T

L

S

S

L

S

N

R

P

T

V

V

F

F

R

G

V

-

G

-

E

-

Q

-

I

-

A

-

E

-

G

N

V

V

R

A

L

L

H

P

L

L

G

E

L

L

S

D

-

N

-

T

-

P

R

K

S

D

D

E

A

V

A

K

A

R

R

R

A

V

V

T

D

E

L

L

R

S

Q

L

V

V

G

G

I

L

P

G

S

D

P

-

E

-

L

-

R

K

A

H

R

D

D

S

F

Y

P

P

H

S

Q

N

M

L

S

S

G

G

M

Q

R

K

Q

Q

R

R

V

V

M

A

I

I

A

A

M

R

A

A

L

L

A

A

C

S

D

N

P

P

R

K

L

V

L

L

I

L

A

C

D

D

E

Q

P

A

T

T

T

S

A

A

L

L

D

D

V

P

T

A

I

T

Q

T

R

R

Q

S

I

I

L

L

R

E

L

L

M

L

R

K

D

D

L

I

A

N

A

R

H

R

R

L

G

G

A

L

S

T

V

I

L

L

I

I

T

T

H

H

D

E

L

M

G

D

V

V

A

K

E

R

T

I

A

C

D

D

H

C

V

V

A

A

V

V

M

I

Y

S

A

N

G

G

R

E

I

L

M

I

E

E

H

Q

A

D

S

T

V

V

G

S

D

E

F

V

F

F

A

S

R

H

P

P

L

K

H

T

P

P

Y

L

A

A

Q

Q

G

K

L

F

M

I

R

Q

S

S

V

T

P

L

Q

H

A

L

-

D

V

I

S

P

G

E

P

D

R

Y

A

Q

E

E

R

R

L

L

S

Q

A

A

I

E

P

P

G

F

T

T

binding adenosine-5'-diphosphate: S41 (= S63), G42 (= G64), A43 (≠ C65), G44 (= G66), K45 (= K67), S46 (= S68), T47 (≠ A73)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 14

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
36% identity, 72% coverage: 41:296/358 of query aligns to 19:264/344 of 6cvlD

query
sites
6cvlD

L
x
I

R

Q

A

A

V

L

A

N

G

N

I

V

S

S

F

L

S

H

I

V

A

P

P

A

G

G

G

Q

T

I

L

Y

C

G

L

V

V

I

G

G

E

A

S
|
S

G
|
G

C
x
A

G
|
G

K
|
K

S
|
S

M

-

T

-

A

-

L

-

A
x
T

L

L

L

I

R

R

L

C

V

V

P

N

E

L

P

L

G

E

R

R

V

P

A

T

S

E

G

G

S

S

V

V

R

L

L

V

E

D

G

G

R

Q

E

E

L

L

L

T

T

T

L

L

P

S

E

E

P

S

E

E

M

L

R

T

G

K

V

A

R

R

G

-

R

R

S

Q

I

I

S

G

M

M

I

I

F

F

Q

Q

E

H

-

F

P

N

M

L

T

L

S

S

L

S

N

R

P

T

V

V

F

F

R

G

V

-

G

-

E

-

Q

-

I

-

A

-

E

-

G

N

V

V

R

A

L

L

H

P

L

L

G

E

L

L

S

D

-

N

-

T

-

P

R

K

S

D

D

E

A

V

A

K

A

R

R

R

A

V

V

T

D

E

L

L

R

S

Q

L

V

V

G

G

I

L

P

G

S

D

P

-

E

-

L

-

R

K

A

H

R

D

D

S

F

Y

P

P

H

S

Q
x
N

M
x
L

S
|
S

G
|
G

M
x
Q

R

K

Q

Q

R

R

V

V

M

A

I

I

A

A

M

R

A

A

L

L

A

A

C

S

D

N

P

P

R

K

L

V

L

L

I

L

A

C

D

D

E

Q

P

A

T

T

T

S

A

A

L

L

D

D

V

P

T

A

I

T

Q

T

R

R

Q

S

I

I

L

L

R

E

L

L

M

L

R

K

D

D

L

I

A

N

A

R

H

R

R

L

G

G

A

L

S

T

V

I

L

L

I

I

T

T

H
|
H

D

E

L

M

G

D

V

V

A

K

E

R

T

I

A

C

D

D

H

C

V

V

A

A

V

V

M

I

Y

S

A

N

G

G

R

E

I

L

M

I

E

E

H

Q

A

D

S

T

V

V

G

S

D

E

F

V

F

F

A

S

R

H

P

P

L

K

H

T

P

P

Y

L

A

A

Q

Q

G

K

L

F

M

I

R

Q

S

S

V

T

P

L

Q

H

A

L

-

D

V

I

S

P

G

E

P

D

R

Y

A

Q

E

E

R

R

L

L

S

Q

A

A

I

E

P

P

G

F

T

T

binding phosphothiophosphoric acid-adenylate ester: I19 (≠ L41), S41 (= S63), G42 (= G64), A43 (≠ C65), G44 (= G66), K45 (= K67), S46 (= S68), T47 (≠ A73), N141 (≠ Q174), L142 (≠ M175), S143 (= S176), G144 (= G177), G145 (= G178), Q146 (≠ M179), H200 (= H233)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 12, 14

7z18I E. Coli c-p lyase bound to a phnk abc dimer and atp (see paper)
35% identity, 68% coverage: 39:280/358 of query aligns to 14:249/250 of 7z18I

query
sites
7z18I

A

A

P

P

L

G

R
x
K

A
x
G

V

F

A

S

G

D

I

V

S

S

F

F

S

D

I

L

A

W

P

P

G

G

G

E

T

V

L

L

C

G

L

I

V

V

G

G

E

E

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
x
T

M
x
T

T

L

A

L

L

K

A

S

L

I

-

S

L

A

R

R

L

L

V

T

P

P

E

Q

P

-

G

-

R

-

V

-

A

-

S

Q

G

G

S

E

V

I

R

H

L

Y

E

E

G

N

R

R

E

S

L

L

L

Y

T

A

L

M

P

S

E

E

P

A

E

D

M

R

R

R

G

R

V

L

R

L

G

R

R

T

S

E

I

W

S

G

M

V

I

V

F

H

Q
|
Q

E

H

P

P

M

L

T

D

S

G

L

L

N

R

P

R

V

Q

F

V

R

S

V

A

G

G

E

G

Q

N

I

I

A

G

E

E

G

R

V

L

R

M

L

A

H

T

L

G

G

A

L

R

S

H

R

Y

S

G

D

D

A

I

A

R

A

A

R

T

A

A

V

Q

D

K

L

W

L

L

R

E

Q

E

V

V

G

E

I

I

P

P

S

A

P

N

E

-

L

-

R
|
R

A

I

R

D

D

D

F

L

P

P

H

T

Q
x
T

M
x
F

S
|
S

G
|
G

M
|
M

R

Q

Q

Q

R

R

V

L

M

Q

I

I

A

A

M

R

A

N

L

L

A

V

C

T

D

H

P

P

R

K

L

L

L

V

I

F

A

M

D

D

E

E

P

P

T

T

T

G

A

G

L

L

D

D

V

V

T

S

I

V

Q

Q

R

A

Q

R

I

L

L

L

R

D

L

L

M

L

R

R

D

G

L

L

A

V

H

E

R

L

G

N

A

L

S

A

V

V

L

V

L

I

I

V

T

T

H
|
H

D

D

L

L

G

G

V

V

V

A

A

R

E

L

T

L

A

A

D

D

H

R

V

L

A

L

V

V

M

M

Y

K

A

Q

G

G

R

Q

I

V

M

V

E

E

H

S

A

G

S

L

V

T

G

D

D

R

F

V

F

L

A

D

R

D

P

P

L

H

H

H

P

P

Y

Y

A

T

Q

Q

G

L

L

L

M

V

R

S

S

S

V

V

P

L

Q

Q

binding adenosine-5'-triphosphate: K17 (≠ R42), G18 (≠ A43), S38 (= S63), G39 (= G64), S40 (≠ C65), G41 (= G66), K42 (= K67), T43 (≠ S68), T44 (≠ M69), Q88 (= Q117), R136 (= R167), T143 (≠ Q174), F144 (≠ M175), S145 (= S176), G146 (= G177), G147 (= G178), M148 (= M179), H202 (= H233)
binding magnesium ion: T43 (≠ S68), Q88 (= Q117)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 13

7z15I E. Coli c-p lyase bound to a phnk/phnl dual abc dimer and adp + pi (see paper)
35% identity, 68% coverage: 39:280/358 of query aligns to 14:249/253 of 7z15I

query
sites
7z15I

A

A

P

P

L

G

R
x
K

A
x
G

V

F

A

S

G

D

I

V

S

S

F

F

S

D

I

L

A

W

P

P

G

G

G

E

T

V

L

L

C

G

L

I

V

V

G

G

E
|
E

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
x
T

M
x
T

T

L

A

L

L

K

A

S

L

I

-

S

L

A

R

R

L

L

V

T

P

P

E

Q

P

-

G

-

R

-

V

-

A

-

S

Q

G

G

S

E

V

I

R

H

L

Y

E

E

G

N

R

R

E

S

L

L

L

Y

T

A

L

M

P

S

E

E

P

A

E

D

M

R

R

R

G

R

V

L

R

L

G

R

R

T

S

E

I

W

S

G

M

V

I

V

F

H

Q
|
Q

E

H

P

P

M

L

T

D

S

G

L

L

N

R

P

R

V

Q

F

V

R

S

V

A

G

G

E

G

Q

N

I

I

A

G

E

E

G

R

V

L

R

M

L

A

H

T

L

G

G

A

L

R

S

H

R

Y

S

G

D

D

A

I

A

R

A

A

R

T

A

A

V

Q

D

K

L

W

L

L

R

E

Q

E

V

V

G

E

I

I

P

P

S

A

P

N

E

-

L

-

R
|
R

A

I

R

D

D

D

F

L

P

P

H

T

Q
x
T

M

F

S
|
S

G

G

M
|
M

R

Q

Q

Q

R

R

V

L

M

Q

I

I

A

A

M

R

A

N

L

L

A

V

C

T

D

H

P

P

R

K

L

L

L

V

I

F

A

M

D
|
D

E

E

P

P

T

T

T

G

A

G

L

L

D

D

V

V

T

S

I

V

Q

Q

R

A

Q

R

I

L

L

L

R

D

L

L

M

L

R

R

D

G

L

L

A

V

H

E

R

L

G

N

A

L

S

A

V

V

L

V

L

I

I

V

T

T

H

H

D

D

L

L

G

G

V

V

V

A

A

R

E

L

T

L

A

A

D

D

H

R

V

L

A

L

V

V

M

M

Y

K

A

Q

G

G

R

Q

I

V

M

V

E

E

H

S

A

G

S

L

V

T

G

D

D

R

F

V

F

L

A

D

R

D

P

P

L

H

H

H

P

P

Y

Y

A

T

Q

Q

G

L

L

L

M

V

R

S

S

S

V

V

P

L

Q

Q

binding adenosine-5'-diphosphate: K17 (≠ R42), G18 (≠ A43), E37 (= E62), S38 (= S63), G39 (= G64), S40 (≠ C65), G41 (= G66), K42 (= K67), T43 (≠ S68), T44 (≠ M69), R136 (= R167), T143 (≠ Q174), S145 (= S176), M148 (= M179)
binding magnesium ion: T43 (≠ S68), Q88 (= Q117), D168 (= D199)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 13

7z16I E. Coli c-p lyase bound to phnk/phnl dual abc dimer with amppnp and phnk e171q mutation (see paper)
34% identity, 68% coverage: 39:280/358 of query aligns to 14:249/250 of 7z16I

query
sites
7z16I

A

A

P

P

L

G

R
x
K

A
x
G

V

F

A

S

G

D

I

V

S

S

F

F

S

D

I

L

A

W

P

P

G

G

G

E

T

V

L

L

C

G

L

I

V

V

G

G

E

E

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
x
T

M
x
T

T

L

A

L

L

K

A

S

L

I

-

S

L

A

R

R

L

L

V

T

P

P

E

Q

P

-

G

-

R

-

V

-

A

-

S

Q

G

G

S

E

V

I

R

H

L

Y

E

E

G

N

R

R

E

S

L

L

L

Y

T

A

L

M

P

S

E

E

P

A

E

D

M

R

R

R

G

R

V

L

R

L

G

R

R

T

S

E

I

W

S

G

M

V

I

V

F

H

Q

Q

E

H

P

P

M

L

T

D

S

G

L

L

N

R

P

R

V

Q

F

V

R

S

V

A

G

G

E

G

Q

N

I

I

A

G

E

E

G

R

V

L

R

M

L

A

H

T

L

G

G

A

L

R

S

H

R

Y

S

G

D

D

A

I

A

R

A

A

R

T

A

A

V

Q

D

K

L

W

L

L

R

E

Q

E

V

V

G

E

I

I

P

P

S

A

P

N

E

-

L

-

R
|
R

A

I

R

D

D

D

F

L

P

P

H

T

Q
x
T

M

F

S
|
S

G
|
G

M
|
M

R

Q

Q

Q

R

R

V

L

M

Q

I

I

A

A

M

R

A

N

L

L

A

V

C

T

D

H

P

P

R

K

L

L

L

V

I

F

A

M

D

D

E
x
Q

P

P

T

T

T

G

A

G

L

L

D

D

V

V

T

S

I

V

Q

Q

R

A

Q

R

I

L

L

L

R

D

L

L

M

L

R

R

D

G

L

L

A

V

H

E

R

L

G

N

A

L

S

A

V

V

L

V

L

I

I

V

T

T

H
|
H

D

D

L

L

G

G

V

V

V

A

A

R

E

L

T

L

A

A

D

D

H

R

V

L

A

L

V

V

M

M

Y

K

A

Q

G

G

R

Q

I

V

M

V

E

E

H

S

A

G

S

L

V

T

G

D

D

R

F

V

F

L

A

D

R

D

P

P

L

H

H

H

P

P

Y

Y

A

T

Q

Q

G

L

L

L

M

V

R

S

S

S

V

V

P

L

Q

Q

binding phosphoaminophosphonic acid-adenylate ester: K17 (≠ R42), G18 (≠ A43), S38 (= S63), G39 (= G64), S40 (≠ C65), G41 (= G66), K42 (= K67), T43 (≠ S68), T44 (≠ M69), R136 (= R167), T143 (≠ Q174), S145 (= S176), G146 (= G177), G147 (= G178), M148 (= M179), Q169 (≠ E200), H202 (= H233)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 13

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
35% identity, 67% coverage: 39:278/358 of query aligns to 13:239/241 of 4u00A

query
sites
4u00A

A

G

P

P

L
|
L

R

H

A
x
V

V

L

A

K

G

G

I

I

S

H

F

L

S

E

I

V

A

A

P

P

G

G

G

E

T

K

L

L

C

V

L

I

V

I

G

G

E

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

M

-

T

-

A

-

L

-

A
x
T

L

L

L

I

R

R

L

T

V

I

P

N

E

R

P

L

G

E

R

D

V

F

A

Q

S

E

G

G

S

E

V

V

R

V

L

V

E

D

G

G

R

-

E

-

L

L

L

S

T

V

L

K

P

D

E

D

P

R

E

A

M

L

R

R

G

E

V

I

R

R

G

-

R

R

S

E

I

V

S

G

M

M

I

V

F

F

Q

Q

E

Q

P

-

M

-

T

F

S

N

L

L

N

F

P

P

V

H

F

M

R

T

V

V

G

L

E

E

Q

N

I

V

A

T

E

L

G

A

V

P

R

M

L

R

H

V

L

R

G

R

L

W

S

P

R

R

S

E

D

K

A

A

A

E

A

K

R

K

A

A

V

L

D

E

L

L

R

E

Q

R

V

V

G

G

I

I

P

L

S

D

P

-

E

-

L

-

R

Q

A

A

R

R

D

K

F

Y

P

P

H

A

Q

Q

M

L

S

S

G

G

M

Q

R

Q

Q

Q

R

R

V

V

M

A

I

I

A

A

M

R

A

A

L

L

A

A

C

M

D

E

P

P

R

K

L

I

L

M

I

L

A

F

D

D

E

E

P

P

T

T

T

S

A

A

L

L

D

D

V

P

T

E

I

M

Q

V

R

G

Q

E

I

V

L

L

R

D

L

V

M

M

R

R

D

D

L

L

A

-

A

A

H

Q

R

G

G

G

A

M

S

T

V

M

L

V

I

V

T

T

H

H

D

E

L

M

G

G

V

F

V

A

A

R

E

E

T

V

A

A

D

D

H

R

V

V

A

V

V

F

M

M

Y

D

A

G

G

G

R

Q

I

I

M

V

E

E

H

E

A

G

S

R

V

P

G

E

D

E

F

I

F

F

A

T

R

R

P

P

L

K

H

E

P

E

Y

R

A

T

Q

R

G

S

L

F

M

L

R

Q

S

R

V

V

binding adenosine-5'-diphosphate: L15 (= L41), V17 (≠ A43), S37 (= S63), G38 (= G64), S39 (≠ C65), G40 (= G66), K41 (= K67), S42 (= S68), T43 (≠ A73)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

7ahhC Opua inhibited inward-facing, sbd docked (see paper)
30% identity, 68% coverage: 44:285/358 of query aligns to 42:273/382 of 7ahhC

query
sites
7ahhC

V

V

A

Y

G

D

I

T

S

N

F

F

S

E

I

I

A

N

P

E

G

G

G

E

T

I

L

F

C

V

L

I

V

M

G

G

E

L

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

M

-

T
|
T

A

L

L

L

A

R

L

L

R

N

L

R

V

L

P

I

E

E

P

P

G

-

R

-

V

-

A

T

S

S

G

G

S

K

V

I

R

F

L

I

E

D

G

N

R

Q

E

D

L

V

L

A

T

T

L

L

P

N

E

K

P

E

E

D

M

L

R

L

G

Q

V

V

R

R

G

R

R

K

S

T

I

M

S

S

M

M

I

V

F

F

Q

Q

E

N

P

-

M

-

T

F

S

G

L

L

N

F

P

P

V

H

F

R

R

T

V

I

G

L

E

E

Q

N

I

T

A

E

E

Y

G

G

V

L

R

E

L

V

H

Q

L

-

G

N

L

V

S

P

R

K

S

E

D

E

A

R

A

K

R

R

A

A

V

E

D

K

L

A

L

L

R

D

Q

N

V

A

G

N

I

L

P

-

S

-

P

-

E

-

L

L

R

D

A

F

R

K

D

D

-

Q

F

Y

P

P

H

K

Q

Q

M

L

S

S

G

G

M

M

R

Q

Q

Q

R

R

V

V

M

G

I

L

A

A

M

R

A

A

L

L

A

A

C

N

D

D

P

P

R

E

L

I

L

L

I

L

A

M

D
|
D

E
|
E

P

A

T

F

T

S

A

A

L

L

D

D

V

P

T

L

I

I

Q

R

R

R

Q

E

I

M

L

Q

R

D

L

E

M

L

R

L

D

E

L

L

A

Q

A

A

H

K

R

F

G

Q

A

K

S

T

V

I

L

I

L

F

I

V

T

S

H

H

D

D

L

L

G

N

V

E

V

A

A

L

E

R

T

I

A

G

D

D

H

R

V

I

A

A

V

I

M

M

Y

K

A

D

G

G

R

K

I

I

M

M

E

Q

H

I

A

G

S

T

V

G

G

E

D

E

F

I

F

L

A

T

R

N

P

P

L

A

H

N

P

D

Y

Y

A

V

Q

K

G

T

L

F

M

V

R

E

S

D

V

T

P

A

Q

E

A

N

V

I

S

M

G
x
I

P
|
P

binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: I272 (≠ G284), P273 (= P285)
binding phosphoaminophosphonic acid-adenylate ester: S61 (= S63), G62 (= G64), S63 (≠ C65), G64 (= G66), K65 (= K67), S66 (= S68), T67 (= T70), D187 (= D199), E188 (= E200)

Sites not aligning to the query:

7aheC Opua inhibited inward facing (see paper)
30% identity, 68% coverage: 44:285/358 of query aligns to 42:273/382 of 7aheC

query
sites
7aheC

V

V

A

Y

G

D

I

T

S

N

F

F

S

E

I

I

A

N

P

E

G

G

G

E

T

I

L

F

C

V

L

I

V

M

G

G

E

L

S

S

G

G

C

S

G

G

K

K

S

S

M

-

T

T

A

L

L

L

A

R

L

L

R

N

L

R

V

L

P

I

E

E

P

P

G

-

R

-

V

-

A

T

S

S

G

G

S

K

V

I

R

F

L

I

E

D

G

N

R

Q

E

D

L

V

L

A

T

T

L

L

P

N

E

K

P

E

E

D

M

L

R

L

G

Q

V

V

R

R

G

R

R

K

S

T

I

M

S

S

M

M

I

V

F

F

Q

Q

E

N

P

-

M

-

T

F

S

G

L

L

N

F

P

P

V

H

F

R

R

T

V

I

G

L

E

E

Q

N

I

T

A

E

E

Y

G

G

V

L

R

E

L

V

H

Q

L

-

G

N

L

V

S

P

R

K

S

E

D

E

A

R

A

K

R

R

A

A

V

E

D

K

L

A

L

L

R

D

Q

N

V

A

G

N

I

L

P

-

S

-

P

-

E

-

L

L

R

D

A

F

R

K

D

D

-

Q

F

Y

P

P

H

K

Q

Q

M

L

S

S

G

G

M

M

R

Q

Q

Q

R

R

V

V

M

G

I

L

A

A

M

R

A

A

L

L

A

A

C

N

D

D

P

P

R

E

L

I

L

L

I

L

A

M

D

D

E

E

P

A

T

F

T

S

A

A

L

L

D

D

V

P

T

L

I

I

Q

R

R

R

Q

E

I

M

L

Q

R

D

L

E

M

L

R

L

D

E

L

L

A

Q

A

A

H

K

R

F

G

Q

A

K

S

T

V

I

L

I

L

F

I

V

T

S

H

H

D

D

L

L

G

N

V

E

V

A

A

L

E

R

T

I

A

G

D

D

H

R

V

I

A

A

V

I

M

M

Y

K

A

D

G

G

R

K

I

I

M

M

E

Q

H

I

A

G

S

T

V

G

G

E

D

E

F

I

F

L

A

T

R

N

P

P

L

A

H

N

P

D

Y

Y

A

V

Q

K

G

T

L

F

M

V

R

E

S

D

V

T

P

A

Q

E

A

N

V

I

S

M

G

I

P

P

Sites not aligning to the query:

binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 295, 296, 297, 298, 357, 372

3c4jA Abc protein artp in complex with atp-gamma-s
30% identity, 73% coverage: 16:278/358 of query aligns to 2:241/242 of 3c4jA

query
sites
3c4jA

R

Q

L

M

L

I

D

D

V

V

R

H

D

Q

L

L

S

K

V

K

E

S

F
|
F

P

-

G

-

R

-

P

-

E

-

A

-

D

-

G

-

T

-

P

-

G

-

A

G

P

S

L

L

R

E

A
x
V

V

L

A

K

G

G

I

I

S

N

F

V

S

H

I

I

A

R

P

E

G

G

G

E

T

V

L

V

C

V

L

V

V

I

G

G

E
x
P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

M

-

T
|
T

A

F

L

L

A

R

L

C

L

L

R

N

L

L

V

L

P

E

E

D

P

-

G

-

R

-

V

F

A

D

S

E

G

G

S

E

V

I

R

I

L

I

E

D

G

G

R

I

E

N

L

L

L

-

T

K

L

A

P

K

E

D

P

T

E

N

M

L

R

N

G

K

V

V

R

R

G

-

R

E

S

E

I

V

S

G

M

M

I

V

F

F

Q

Q

E

R

-

F

-

N

-

L

-

F

-

P

P

H

M

M

T

T

S

V

L

L

N

N

P

N

V

I

F

T

R

L

V

A

G

P

E

M

Q

K

I

V

A

R

E

K

G

-

V

-

R

-

L

-

H

-

L

-

G

-

L

W

S

P

R

R

S

E

D

K

A

A

A

E

A

A

R

K

A

A

V

M

D

E

L

L

R

D

Q

K

V

V

G

G

I

L

P

K

S

D

P

-

E

-

L

-

R

K

A

A

R
x
H

D

A

F
x
Y

P

P

H

D

Q

S

M

L

S

S

G

G

M

Q

R

A

Q

Q

R

R

V

V

M

A

I

I

A

A

M

R

A

A

L

L

A

A

C

M

D

E

P

P

R

K

L

I

L

M

I

L

A

F

D

D

E
|
E

P

P

T

T

T

S

A

A

L

L

D

D

V

P

T

E

I

M

Q

V

R

G

Q

E

I

V

L

L

R

S

L

V

M

M

R

K

D

Q

L

L

A

-

A

A

H

N

R

E

G

G

A

M

S

T

V

M

L

V

I

V

T

T

H

H

D

E

L

M

G

G

V

F

V

A

A

R

E

E

T

V

A

G

D

D

H

R

V

V

A

L

V

F

M

M

Y

D

A

G

G

G

R

Y

I

I

M

I

E

E

H

E

A

G

S

K

V

P

G

E

D

D

F

L

F

F

A

D

R

R

P

P

L

Q

H

H

P

E

Y

R

A

T

Q

K

G

A

L

F

M

L

R

S

S

K

V

V

binding phosphothiophosphoric acid-adenylate ester: F13 (= F27), V18 (≠ A43), P37 (≠ E62), S38 (= S63), G39 (= G64), S40 (≠ C65), G41 (= G66), K42 (= K67), S43 (= S68), T44 (= T70), H133 (≠ R169), Y135 (≠ F171), E164 (= E200)

3c41J Abc protein artp in complex with amp-pnp/mg2+
30% identity, 73% coverage: 16:278/358 of query aligns to 2:241/242 of 3c41J

query
sites
3c41J

R

Q

L

M

L

I

D

D

V

V

R

H

D

Q

L

L

S

K

V

K

E

S

F
|
F

P

-

G

-

R

-

P

-

E

-

A

-

D

-

G

-

T

-

P

-

G

-

A

G

P

S

L

L

R

E

A
x
V

V

L

A

K

G

G

I

I

S

N

F

V

S

H

I

I

A

R

P

E

G

G

G

E

T

V

L

V

C

V

L

V

V

I

G

G

E
x
P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

M

-

T
|
T

A

F

L

L

A
x
R

L

C

L

L

R

N

L

L

V

L

P

E

E

D

P

-

G

-

R

-

V

F

A

D

S

E

G

G

S

E

V

I

R

I

L

I

E

D

G

G

R

I

E

N

L

L

L

-

T

K

L

A

P

K

E

D

P

T

E

N

M

L

R

N

G

K

V

V

R

R

G

-

R

E

S

E

I

V

S

G

M

M

I

V

F

F

Q

Q

E

R

-

F

-

N

-

L

-

F

-

P

P

H

M

M

T

T

S

V

L

L

N

N

P

N

V

I

F

T

R

L

V

A

G

P

E

M

Q

K

I

V

A

R

E

K

G

-

V

-

R

-

L

-

H

-

L

-

G

-

L

W

S

P

R

R

S

E

D

K

A

A

A

E

A

A

R

K

A

A

V

M

D

E

L

L

R

D

Q

K

V

V

G

G

I

L

P

K

S

D

P

-

E

-

L

-

R

K

A

A

R
x
H

D

A

F
x
Y

P

P

H

D

Q

S

M

L

S

S

G

G

M

Q

R

A

Q

Q

R

R

V

V

M

A

I

I

A

A

M

R

A

A

L

L

A

A

C

M

D

E

P

P

R

K

L

I

L

M

I

L

A

F

D
|
D

E

E

P

P

T

T

T

S

A

A

L

L

D

D

V

P

T

E

I

M

Q

V

R

G

Q

E

I

V

L

L

R

S

L

V

M

M

R

K

D

Q

L

L

A

-

A

A

H

N

R

E

G

G

A

M

S

T

V

M

L

V

I

V

T

T

H

H

D

E

L

M

G

G

V

F

V

A

A

R

E

E

T

V

A

G

D

D

H

R

V

V

A

L

V

F

M

M

Y

D

A

G

G

G

R

Y

I

I

M

I

E

E

H

E

A

G

S

K

V

P

G

E

D

D

F

L

F

F

A

D

R

R

P

P

L

Q

H

H

P

E

Y

R

A

T

Q

K

G

A

L

F

M

L

R

S

S

K

V

V

binding phosphoaminophosphonic acid-adenylate ester: F13 (= F27), V18 (≠ A43), P37 (≠ E62), S38 (= S63), G39 (= G64), S40 (≠ C65), G41 (= G66), K42 (= K67), S43 (= S68), T44 (= T70), R47 (≠ A73), H133 (≠ R169), Y135 (≠ F171)
binding magnesium ion: S43 (= S68), D163 (= D199)

2olkA Abc protein artp in complex with adp-beta-s
30% identity, 73% coverage: 16:278/358 of query aligns to 2:241/242 of 2olkA

query
sites
2olkA

R

Q

L

M

L

I

D

D

V

V

R

H

D

Q

L

L

S

K

V

K

E

S

F
|
F

P

-

G

-

R

-

P

-

E

-

A

-

D

-

G

-

T

-

P

-

G

-

A

G

P

S

L
|
L

R

E

A
x
V

V

L

A

K

G

G

I

I

S

N

F

V

S

H

I

I

A

R

P

E

G

G

G

E

T

V

L

V

C

V

L

V

V

I

G

G

E

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

M

-

T
|
T

A

F

L

L

A

R

L

C

L

L

R

N

L

L

V

L

P

E

E

D

P

-

G

-

R

-

V

F

A

D

S

E

G

G

S

E

V

I

R

I

L

I

E

D

G

G

R

I

E

N

L

L

L

-

T

K

L

A

P

K

E

D

P

T

E

N

M

L

R

N

G

K

V

V

R

R

G

-

R

E

S

E

I

V

S

G

M

M

I

V

F

F

Q

Q

E

R

-

F

-

N

-

L

-

F

-

P

P

H

M

M

T

T

S

V

L

L

N

N

P

N

V

I

F

T

R

L

V

A

G

P

E

M

Q

K

I

V

A

R

E

K

G

-

V

-

R

-

L

-

H

-

L

-

G

-

L

W

S

P

R

R

S

E

D

K

A

A

A

E

A

A

R

K

A

A

V

M

D

E

L

L

R

D

Q

K

V

V

G

G

I

L

P

K

S

D

P

-

E

-

L

-

R

K

A

A

R

H

D

A

F

Y

P

P

H

D

Q

S

M

L

S

S

G

G

M

Q

R

A

Q

Q

R

R

V

V

M

A

I

I

A

A

M

R

A

A

L

L

A

A

C

M

D

E

P

P

R

K

L

I

L

M

I

L

A

F

D

D

E

E

P

P

T

T

T

S

A

A

L

L

D

D

V

P

T

E

I

M

Q

V

R

G

Q

E

I

V

L

L

R

S

L

V

M

M

R

K

D

Q

L

L

A

-

A

A

H

N

R

E

G

G

A

M

S

T

V

M

L

V

I

V

T

T

H

H

D

E

L

M

G

G

V

F

V

A

A

R

E

E

T

V

A

G

D

D

H

R

V

V

A

L

V

F

M

M

Y

D

A

G

G

G

R

Y

I

I

M

I

E

E

H

E

A

G

S

K

V

P

G

E

D

D

F

L

F

F

A

D

R

R

P

P

L

Q

H

H

P

E

Y

R

A

T

Q

K

G

A

L

F

M

L

R

S

S

K

V

V

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (= F27), L16 (= L41), V18 (≠ A43), S38 (= S63), G39 (= G64), S40 (≠ C65), G41 (= G66), K42 (= K67), S43 (= S68), T44 (= T70)

2oljA Abc protein artp in complex with adp/mg2+
30% identity, 73% coverage: 16:278/358 of query aligns to 2:241/242 of 2oljA

query
sites
2oljA

R

Q

L

M

L

I

D

D

V

V

R

H

D

Q

L

L

S

K

V

K

E

S

F
|
F

P

-

G

-

R

-

P

-

E

-

A

-

D

-

G

-

T

-

P

-

G

-

A

G

P

S

L

L

R

E

A
x
V

V

L

A

K

G

G

I

I

S

N

F

V

S

H

I

I

A

R

P

E

G

G

G

E

T

V

L

V

C

V

L

V

V

I

G

G

E

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

M

-

T
|
T

A

F

L

L

A

R

L

C

L

L

R

N

L

L

V

L

P

E

E

D

P

-

G

-

R

-

V

F

A

D

S

E

G

G

S

E

V

I

R

I

L

I

E

D

G

G

R

I

E

N

L

L

L

-

T

K

L

A

P

K

E

D

P

T

E

N

M

L

R

N

G

K

V

V

R

R

G

-

R

E

S

E

I

V

S

G

M

M

I

V

F

F

Q

Q

E

R

-

F

-

N

-

L

-

F

-

P

P

H

M

M

T

T

S

V

L

L

N

N

P

N

V

I

F

T

R

L

V

A

G

P

E

M

Q

K

I

V

A

R

E

K

G

-

V

-

R

-

L

-

H

-

L

-

G

-

L

W

S

P

R

R

S

E

D

K

A

A

A

E

A

A

R

K

A

A

V

M

D

E

L

L

R

D

Q

K

V

V

G

G

I

L

P

K

S

D

P

-

E

-

L

-

R

K

A

A

R
x
H

D

A

F
x
Y

P

P

H

D

Q

S

M

L

S

S

G

G

M

Q

R

A

Q

Q

R

R

V

V

M

A

I

I

A

A

M

R

A

A

L

L

A

A

C

M

D

E

P

P

R

K

L

I

L

M

I

L

A

F

D

D

E

E

P

P

T

T