SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 8500662 FitnessBrowser__Miya:8500662 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P10880 Shikimate kinase 2; SK 2; Shikimate kinase II; SKII; EC 2.7.1.71 from Dickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi) (see paper)
50% identity, 93% coverage: 11:175/177 of query aligns to 5:168/173 of P10880

query
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P10880

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C13 (≠ S19) mutation to S: 66% of wild-type activity. Increase in substrates affinity.
K15 (= K21) mutation to M: Loss of activity. Increased thermostability.
D34 (= D40) mutation to N: Loss of activity.
C162 (≠ E169) mutation to S: No effect on activity and substrates affinity.

1e6cA K15m mutant of shikimate kinase from erwinia chrysanthemi (see paper)
52% identity, 85% coverage: 11:160/177 of query aligns to 5:153/170 of 1e6cA

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1e6cA

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binding phosphate ion: R11 (= R17), G12 (≠ A18), M15 (≠ K21), T16 (= T22)

2shkB The three-dimensional structure of shikimate kinase from erwinia chrysanthemi complexed with adp (see paper)
50% identity, 85% coverage: 11:160/177 of query aligns to 5:143/162 of 2shkB

query
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binding adenosine-5'-diphosphate: G12 (≠ A18), C13 (≠ S19), G14 (= G20), K15 (= K21), T16 (= T22), T17 (= T23), R110 (= R117)
binding magnesium ion: Q134 (≠ R151), A137 (= A154)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 145, 147

1shkA The three-dimensional structure of shikimate kinase from erwinia chrysanthemi (see paper)
47% identity, 85% coverage: 11:160/177 of query aligns to 5:138/158 of 1shkA

query
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binding magnesium ion: R92 (= R99), A93 (≠ D100), G95 (= G102), Q129 (≠ R151), A132 (= A154)

4y0aA Shikimate kinase from acinetobacter baumannii in complex with shikimate (see paper)
40% identity, 86% coverage: 11:162/177 of query aligns to 11:159/179 of 4y0aA

query
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binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: M17 (≠ R17), D40 (= D40), F63 (= F63), R64 (= R64), G86 (= G86), G87 (= G87), L126 (≠ S129), R143 (= R146)
binding sulfate ion: G18 (≠ A18), A19 (≠ S19), G20 (= G20), K21 (= K21), T22 (= T22), E112 (≠ D112), R124 (= R127), R137 (≠ A140)

P07547 Pentafunctional AROM polypeptide; EC 4.2.3.4; EC 2.5.1.19; EC 2.7.1.71; EC 4.2.1.10; EC 1.1.1.25 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see 2 papers)
31% identity, 85% coverage: 6:156/177 of query aligns to 861:1009/1583 of P07547

query
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P07547

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Sites not aligning to the query:

44:46 binding
81:84 binding
114:116 binding
119 binding
139:140 binding
161 binding
179:182 binding
190 binding
194 binding
271 binding
287 binding

6hqvA Pentafunctional arom complex from chaetomium thermophilum (see paper)
31% identity, 84% coverage: 9:156/177 of query aligns to 843:989/1555 of 6hqvA

query
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binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D874 (= D40), G923 (= G86), G924 (= G87), R979 (= R146)

Sites not aligning to the query:

active site: 123, 145, 187, 243, 253, 257, 261, 264, 268, 280
binding (4S,5R)-4,5-dihydroxy-3-oxocyclohex-1-ene-1-carboxylic acid: 1060, 1062, 1181, 1224, 1232, 1242, 1243
binding glutamic acid: 139, 145, 187, 243, 257, 264, 280
binding nicotinamide-adenine-dinucleotide: 42, 44, 45, 76, 79, 107, 108, 109, 112, 132, 133, 135, 139, 140, 145, 154, 175, 176, 177, 180, 280
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: 413, 562, 563, 1277, 1279, 1323, 1327, 1348, 1368, 1526
binding zinc ion: 187, 264, 280

7tbvA Crystal structure of the shikimate kinase + 3-dehydroquinate dehydratase + 3-dehydroshikimate dehydrogenase domains of aro1 from candida albicans (see paper)
27% identity, 83% coverage: 9:155/177 of query aligns to 4:134/682 of 7tbvA

query
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L

A

E

L

K

A

L

G

G

M

T

T

G

V

I

E

K

Q

S

I

L

V

I

E

K

R

A

E

K

G

G

W

W

E

E

G

Y

F

F

R

R

R

Q

R

E

E

E

T

A

E

I

A

V

L

A

R

K

A

E

-

C

-

F

T

T

L

K

G

F

H

S

D

K

G

G

L

Y

V

V

V

L

A

S

T

T

G

G

M

I

V

V

L

E

A

G

E

E

E

D

N

A

R

R

R

Q

M

Q

L

L

R

K

-

S

-

Y

-

A

-

D

D

N

G

G

L

I

V

V

A

L

Y

H

L

L

C

H

G

R

S

D

V

L

D

D

L

E

L

-

A

-

G

-

R

-

L

-

A

-

R

-

D

-

P

-

L

-

A

-

Q

-

R

-

P

-

S

-

L

-

T

-

G

-

R

-

P

-

I

-

A

S

D

S

E

E

V

V

A

Q

E

E

V

V

L

W

A

L

A

R

R

R

E

E

P

K

L

W

Y

Y

R

H

E

E

C

C

A

S

H

N

Sites not aligning to the query:

binding magnesium ion: 300, 302, 329

1zyuA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with shikimate and amppcp at 2.85 angstrom resolution (see paper)
36% identity, 86% coverage: 8:159/177 of query aligns to 1:148/168 of 1zyuA

query
sites
1zyuA

A

A

R

P

R

K

V

A

Y

V

L

L

V

V

G

G

P

L

R
x
P

A
x
G

S

S

G
|
G

K
|
K

T
x
S

T
|
T

V

I

G

G

K

R

A

R

L

L

A

A

A

K

R

A

A

L

G

G

W

V

H

G

F

L

E

L

D

D

T

T

D
|
D

A

V

T

A

V

I

A

E

A

Q

L

R

A

T

G

G

M

R

T

S

V

I

E

A

Q

D

I

I

V

F

E

A

R

T

E

D

G

G

W

E

E

Q

G

E

F

F

R
|
R

R

R

R

I

E

E

T

E

E

D

A

V

L

V

R

R

A

A

T

A

L

L

G

A

-

D

H

H

D

D

G

G

L

-

V

V

V

L

A

S

T

L

G

G

G
|
G

M

A

V

V

L

T

A

S

E

P

E

G

N

V

R

R

R

A

M

A

L

L

R

-

D

A

G

G

G

H

L

T

V

V

A

V

Y

Y

L

L

C

E

G

I

S

S

V

A

D

-

L

-

A

A

G

E

R

G

L

V

A

R

R
|
R

D

T

P

G

L

G

A

N

A

T

Q

V

R

R

P
|
P

S
x
L

L

L

T

A

G

G

R

P

P

D

I

R

A

A

D

E

E

K

V

Y

A

R

E

A

V

L

L

M

A

A

A

K

R
|
R

E

A

P

P

L

L

Y

Y

R

R

E

R

C

V

A

A

H

T

V

M

V

R

L

V

D

D

binding phosphomethylphosphonic acid adenylate ester: P10 (≠ R17), G11 (≠ A18), G13 (= G20), K14 (= K21), S15 (≠ T22), T16 (= T23), R109 (= R120)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D40), R57 (= R64), G80 (= G87), P117 (= P128), L118 (≠ S129), R135 (= R146)

Sites not aligning to the query:

binding phosphomethylphosphonic acid adenylate ester: 149, 152, 154

4bqsA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with adp and a shikimic acid derivative. (see paper)
36% identity, 86% coverage: 8:159/177 of query aligns to 1:148/169 of 4bqsA

query
sites
4bqsA

A

A

R

P

R

K

V

A

Y

V

L

L

V

V

G

G

P

L

R
x
P

A
x
G

S

S

G
|
G

K
|
K

T
x
S

T
|
T

V

I

G

G

K

R

A

R

L

L

A

A

A

K

R

A

A

L

G

G

W

V

H

G

F

L

E

L

D

D

T

T

D
|
D

A

V

T

A

V

I

A

E

A

Q

L

R

A

T

G

G

M

R

T

S

V

I

E

A

Q

D

I

I

V

F

E

A

R

T

E

D

G

G

W

E

E

Q

G

E

F

F

R

R

R

I

E

E

T

E

E

D

A

V

L

V

R

R

A

A

T

A

L

L

G

A

-

D

H

H

D

D

G

G

L

-

V

V

V

L

A

S

T

L

G

G

M

A

V

V

L

T

A

S

E

P

E

G

N

V

R

R

R

A

M

A

L

L

R

-

D

A

G

G

G

H

L

T

V

V

A

V

Y

Y

L

L

C

E

G

I

S

S

V

A

D

-

L

-

A

A

G

E

R

G

L

V

A

R

R
|
R

D

T

P

G

L

G

A

N

A

T

Q

V

R

R

P

P

S

L

L

L

T

A

G

G

R

P

P

D

I

R

A

A

D

E

E

K

V

Y

A

R

E

A

V

L

L

M

A

A

A

K

R
|
R

E

A

P

P

L

L

Y

Y

R

R

E

R

C

V

A

A

H

T

V

M

V

R

L

V

D

D

binding adenosine-5'-diphosphate: P10 (≠ R17), G11 (≠ A18), G13 (= G20), K14 (= K21), S15 (≠ T22), T16 (= T23), R109 (= R120)
binding (1R,6R,10S)-6,10-dihydroxy-2-oxabicyclo[4.3.1]deca-4(Z),7-diene-8-carboxylic acid: D33 (= D40), R135 (= R146)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 152, 154

2iyyA Shikimate kinase from mycobacterium tuberculosis in complex with shikimate-3-phosphate and so4 (see paper)
36% identity, 86% coverage: 8:159/177 of query aligns to 1:148/169 of 2iyyA

query
sites
2iyyA

A

A

R

P

R

K

V

A

Y

V

L

L

V

V

G

G

P

L

R

P

A

G

S

S

G

G

K
|
K

T

S

T

T

V

I

G

G

K

R

A

R

L

L

A

A

A

K

R

A

A

L

G

G

W

V

H

G

F

L

E

L

D

D

T

T

D
|
D

A

V

T

A

V

I

A
x
E

A

Q

L

R

A

T

G

G

M

R

T
x
S

V
x
I

E

A

Q

D

I

I

V

F

E

A

R

T

E

D

G

G

W

E

E

Q

G

E

F

F

R
|
R

R

R

R

I

E

E

T

E

E

D

A

V

L

V

R

R

A

A

T

A

L

L

G

A

-

D

H

H

D

D

G

G

L

-

V

V

V

L

A

S

T

L

G

G

G
|
G

M

A

V

V

L

T

A

S

E

P

E

G

N

V

R

R

R

A

M

A

L

L

R

-

D

A

G

G

G

H

L

T

V

V

A

V

Y

Y

L

L

C

E

G

I

S

S

V

A

D

-

L

-

A

A

G

E

R

G

L

V

A

R

R

R

D

T

P

G

L

G

A

N

A

T

Q

V

R

R

P

P

S
x
L

L

L

T

A

G

G

R

P

P

D

I

R

A

A

D

E

E

K

V

Y

A

R

E

A

V

L

L

M

A

A

A

K

R
|
R

E

A

P

P

L

L

Y

Y

R

R

E

R

C

V

A

A

H

T

V

M

V

R

L

V

D

D

binding magnesium ion: E37 (≠ A44), S43 (≠ T50), I44 (≠ V51)
binding phosphate ion: K14 (= K21), G79 (= G86)
binding shikimate-3-phosphate: K14 (= K21), D33 (= D40), R57 (= R64), G79 (= G86), G80 (= G87), L118 (≠ S129), R135 (= R146)

3bafA Crystal structure of shikimate kinase from mycobacterium tuberculosis in complex with amp-pnp
36% identity, 86% coverage: 8:159/177 of query aligns to 1:148/165 of 3bafA

query
sites
3bafA

A

A

R

P

R

K

V

A

Y

V

L

L

V

V

G

G

P

L

R
x
P

A

G

S

S

G
|
G

K
|
K

T
x
S

T
|
T

V

I

G

G

K

R

A

R

L

L

A

A

A

K

R

A

A

L

G

G

W

V

H

G

F

L

E

L

D

D

T

T

D
|
D

A

V

T

A

V

I

A

E

A

Q

L

R

A

T

G

G

M

R

T

S

V

I

E

A

Q

D

I

I

V

F

E

A

R

T

E

D

G

G

W

E

E

Q

G

E

F

F

R

R

R

I

E

E

T

E

E

D

A

V

L

V

R

R

A

A

T

A

L

L

G

A

-

D

H

H

D

D

G

G

L

-

V

V

V

L

A

S

T

L

G

G

M

A

V

V

L

T

A

S

E

P

E

G

N

V

R

R

R

A

M

A

L

L

R

-

D

A

G

G

G

H

L

T

V

V

A

V

Y

Y

L

L

C

E

G

I

S

S

V

A

D

-

L

-

A

A

G

E

R

G

L

V

A

R

R
|
R

D

T

P

G

L

G

A

N

A

T

Q

V

R
|
R

P

P

S

L

L

L

T

A

G

G

R

P

P

D

I

R

A

A

D

E

E

K

V

Y

A

R

E

A

V

L

L

M

A

A

A

K

R
|
R

E

A

P

P

L

L

Y

Y

R

R

E

R

C

V

A

A

H

T

V

M

V

R

L

V

D

D

binding phosphoaminophosphonic acid-adenylate ester: P10 (≠ R17), G13 (= G20), K14 (= K21), S15 (≠ T22), T16 (= T23), R109 (= R120), R116 (= R127)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D40), R135 (= R146)

2dfnA Structure of shikimate kinase from mycobacterium tuberculosis complexed with adp and shikimate at 1.9 angstrons of resolution (see paper)
36% identity, 86% coverage: 8:159/177 of query aligns to 1:148/165 of 2dfnA

query
sites
2dfnA

A

A

R

P

R

K

V

A

Y

V

L

L

V

V

G

G

P

L

R
x
P

A
x
G

S
|
S

G
|
G

K
|
K

T
x
S

T
|
T

V

I

G

G

K

R

A

R

L

L

A

A

A

K

R

A

A

L

G

G

W

V

H

G

F

L

E

L

D

D

T

T

D
|
D

A

V

T

A

V

I

A

E

A

Q

L

R

A

T

G

G

M

R

T

S

V
x
I

E

A

Q

D

I

I

V

F

E

A

R

T

E

D

G

G

W

E

E

Q

G

E

F
|
F

R
|
R

R

R

R

I

E

E

T

E

E

D

A

V

L

V

R

R

A

A

T

A

L

L

G

A

-

D

H

H

D

D

G

G

L

-

V

V

V

L

A

S

T

L

G
|
G

M

A

V

V

L

T

A

S

E

P

E

G

N

V

R

R

R

A

M

A

L

L

R

-

D

A

G

G

G

H

L

T

V

V

A

V

Y

Y

L

L

C

E

G

I

S

S

V

A

D

-

L

-

A

A

G

E

R

G

L

V

A

R

R
|
R

D

T

P

G

L

G

A

N

A

T

Q

V

R
|
R

P

P

S

L

L

L

T

A

G

G

R

P

P

D

I

R

A

A

D

E

E

K

V

Y

A

R

E

A

V

L

L

M

A

A

A

K

R
|
R

E

A

P

P

L

L

Y

Y

R

R

E

R

C

V

A

A

H

T

V

M

V

R

L

V

D

D

binding adenosine-5'-diphosphate: G11 (≠ A18), S12 (= S19), G13 (= G20), K14 (= K21), S15 (≠ T22), T16 (= T23), R109 (= R120), R116 (= R127)
binding chloride ion: P10 (≠ R17), K14 (= K21), G79 (= G86)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D40), I44 (≠ V51), F56 (= F63), R57 (= R64), G78 (= G85), G79 (= G86), G80 (= G87), R135 (= R146)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 152, 154

1we2A Crystal structure of shikimate kinase from mycobacterium tuberculosis in complex with mgadp and shikimic acid (see paper)
36% identity, 86% coverage: 8:159/177 of query aligns to 1:148/165 of 1we2A

query
sites
1we2A

A

A

R

P

R

K

V

A

Y

V

L

L

V

V

G

G

P

L

R
x
P

A
x
G

S

S

G
|
G

K
|
K

T
x
S

T
|
T

V

I

G

G

K

R

A

R

L

L

A

A

A

K

R

A

A

L

G

G

W

V

H

G

F

L

E

L

D

D

T

T

D
|
D

A

V

T

A

V

I

A

E

A

Q

L

R

A

T

G

G

M

R

T

S

V
x
I

E

A

Q

D

I

I

V
x
F

E

A

R

T

E

D

G

G

W

E

E

Q

G

E

F

F

R

R

R

I

E

E

T

E

E

D

A

V

L

V

R

R

A

A

T

A

L

L

G

A

-

D

H

H

D

D

G

G

L

-

V

V

V

L

A

S

T

L

G
|
G

M

A

V

V

L

T

A

S

E

P

E

G

N

V

R

R

R

A

M

A

L

L

R

-

D

A

G

G

G

H

L

T

V

V

A

V

Y

Y

L

L

C

E

G

I

S

S

V

A

D

-

L

-

A

A

G

E

R

G

L

V

A

R

R
|
R

D

T

P

G

L

G

A

N

A

T

Q

V

R
|
R

P

P

S
x
L

L

L

T

A

G

G

R

P

P

D

I

R

A

A

D

E

E

K

V

Y

A

R

E

A

V

L

L

M

A

A

A

K

R
|
R

E

A

P

P

L

L

Y

Y

R

R

E

R

C

V

A

A

H

T

V

M

V

R

L

V

D

D

binding adenosine-5'-diphosphate: P10 (≠ R17), G11 (≠ A18), G13 (= G20), K14 (= K21), S15 (≠ T22), T16 (= T23), R109 (= R120), R116 (= R127)
binding 3-dehydroshikimate: D33 (= D40), I44 (≠ V51), F48 (≠ V55), G78 (= G85), G79 (= G86), G80 (= G87), L118 (≠ S129), R135 (= R146)

2iywA Shikimate kinase from mycobacterium tuberculosis in complex with mgatp, open lid (conf. B) (see paper)
36% identity, 86% coverage: 8:159/177 of query aligns to 1:148/178 of 2iywA

query
sites
2iywA

A

A

R

P

R

K

V

A

Y

V

L

L

V

V

G

G

P

L

R

P

A
x
G

S

S

G
|
G

K
|
K

T
x
S

T
|
T

V

I

G

G

K

R

A

R

L

L

A

A

A

K

R

A

A

L

G

G

W

V

H

G

F

L

E

L

D

D

T

T

D

D

A

V

T

A

V

I

A

E

A

Q

L

R

A

T

G

G

M

R

T

S

V

I

E

A

Q

D

I

I

V

F

E

A

R

T

E

D

G

G

W

E

E

Q

G

E

F

F

R

R

R

I

E

E

T

E

E

D

A

V

L

V

R

R

A

A

T

A

L

L

G

A

-

D

H

H

D

D

G

G

L

-

V

V

V

L

A

S

T

L

G

G

M

A

V

V

L

T

A

S

E

P

E

G

N

V

R

R

R

A

M

A

L

L

R

-

D

A

G

G

G

H

L

T

V

V

A

V

Y

Y

L

L

C

E

G

I

S

S

V

A

D

-

L

-

A

A

G

E

R

G

L

V

A

R

R
|
R

D

T

P

G

L

G

A

N

A

T

Q

V

R

R

P

P

S

L

L

L

T

A

G

G

R

P

P

D

I

R

A

A

D

E

E

K

V

Y

A

R

E

A

V

L

L

M

A

A

A

K

R

R

E

A

P

P

L

L

Y

Y

R

R

E

R

C

V

A

A

H

T

V

M

V

R

L

V

D

D

binding adenosine-5'-triphosphate: G11 (≠ A18), G13 (= G20), K14 (= K21), S15 (≠ T22), T16 (= T23), R109 (= R120)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 152, 154

2iyvA Shikimate kinase from mycobacterium tuberculosis in complex with adp, open lid (conf. B) (see paper)
36% identity, 86% coverage: 8:159/177 of query aligns to 1:148/179 of 2iyvA

query
sites
2iyvA

A

A

R

P

R

K

V

A

Y

V

L

L

V

V

G

G

P

L

R

P

A
x
G

S

S

G
|
G

K
|
K

T
x
S

T
|
T

V

I

G

G

K

R

A

R

L

L

A

A

A

K

R

A

A

L

G

G

W

V

H

G

F

L

E

L

D

D

T

T

D

D

A

V

T

A

V

I

A

E

A

Q

L

R

A

T

G

G

M

R

T

S

V

I

E

A

Q

D

I

I

V

F

E

A

R

T

E

D

G

G

W

E

E

Q

G

E

F

F

R

R

R

I

E

E

T

E

E

D

A

V

L

V

R

R

A

A

T

A

L

L

G

A

-

D

H

H

D

D

G

G

L

-

V

V

V

L

A

S

T

L

G

G

M

A

V

V

L

T

A

S

E

P

E

G

N

V

R

R

R

A

M

A

L

L

R

-

D

A

G

G

G

H

L

T

V

V

A

V

Y

Y

L

L

C

E

G

I

S

S

V

A

D

-

L

-

A

A

G

E

R

G

L

V

A

R

R
|
R

D

T

P

G

L

G

A

N

A

T

Q

V

R

R

P

P

S

L

L

L

T

A

G

G

R

P

P

D

I

R

A

A

D

E

E

K

V

Y

A

R

E

A

V

L

L

M

A

A

A

K

R

R

E

A

P

P

L

L

Y

Y

R

R

E

R

C

V

A

A

H

T

V

M

V

R

L

V

D

D

binding adenosine-5'-diphosphate: G11 (≠ A18), G13 (= G20), K14 (= K21), S15 (≠ T22), T16 (= T23), R109 (= R120)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 152, 154

1u8aA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with shikimate and adp at 2.15 angstrom resolution (see paper)
35% identity, 86% coverage: 8:159/177 of query aligns to 1:146/163 of 1u8aA

query
sites
1u8aA

A

A

R

P

R

K

V

A

Y

V

L

L

V

V

G

G

P

L

R

P

A
x
G

S

S

G
|
G

K
|
K

T
x
S

T
|
T

V

I

G

G

K

R

A

R

L

L

A

A

A

K

R

A

A

L

G

G

W

V

H

G

F

L

E

L

D

D

T

T

D
|
D

A

V

T

A

V

I

A

E

A

Q

L

R

A

T

G

G

M

R

T

S

V

I

E

A

Q

D

I

I

V
x
F

E

A

R

T

E

D

G

G

W

E

E

Q

G

E

F

F

R
|
R

R

R

R

I

E

E

T

E

E

D

A

V

L

V

R

R

A

A

T

A

L

L

G

A

-

D

H

H

D

D

G

G

L

-

V

V

V

L

A

S

T

L

G

G

G
|
G

M

A

V

V

L

T

A

S

E

P

E

G

N

V

R

R

R

A

M

A

L

L

R

-

D

A

G

G

G

H

L

T

V

V

A

V

Y

Y

L

L

C

-

G

-

S

-

V

-

D

E

L

I

L

S

A

A

G

A

R

E

L

G

A

V

R

R

D
x
R

P

-

L

T

A

G

Q

V

R

R

P

P

S
x
L

L

L

T

A

G

G

R

P

P

D

I

R

A

A

D

E

E

K

V

Y

A

R

E

A

V

L

L

M

A

A

A

K

R
|
R

E

A

P

P

L

L

Y

Y

R

R

E

R

C

V

A

A

H

T

V

M

V

R

L

V

D

D

binding adenosine-5'-diphosphate: G11 (≠ A18), G13 (= G20), K14 (= K21), S15 (≠ T22), T16 (= T23), R109 (≠ D121)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D40), F48 (≠ V55), R57 (= R64), G79 (= G86), G80 (= G87), L116 (≠ S129), R133 (= R146)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 150, 152

3n2eA Crystal structure of helicobactor pylori shikimate kinase in complex with nsc162535 (see paper)
30% identity, 86% coverage: 9:160/177 of query aligns to 2:146/168 of 3n2eA

query
sites
3n2eA

R

Q

R

H

V

L

Y

V

L

L

V

I

G

G

P
x
F

R

M

A

G

S

S

G

G

K

K

T

S

V

L

G

A

K

Q

A

E

L

L

A

G

A

L

R

A

A

L

G

K

W

L

H

E

F

V

E

L

D

D

T

T

D

D

A

M

T

I

V

I

A

S

A

E

L

R

A

V

G

G

M

L

T

S

V

V

E
x
R

Q

E

I

I

V
x
F

E

E

R

E

E

L

G

G

W

E

E

D

G

N

F

F

R
|
R

R

M

R

F

E

E

T

K

E

N

A

L

L

I

R

D

-

E

-

L

A

K

T

T

L

L

G

K

H

T

D

P

G

H

L

-

V

V

V

I

A

S

T

T

G

G

G
|
G

M

I

V

V

L
x
M

A

H

E

E

E

N

N

-

R

-

M

-

L

L

R

K

D

G

G

L

G

G

L

T

V

T

A

F

Y

Y

L

L

C

K

G

M

S

D

V

F

D

E

L

T

L

L

A

I

G

K

R

R

L

L

A

N

R

Q

D

K

P

E

L

-

A

R

A

A

Q

K

R
|
R

P

P

S

L

L

L

T

N

G

N

R

L

P

T

I

Q

A

A

D

K

E

E

V

L

A

F

E

E

V

-

L

-

A

-

A

K

R
|
R

E

Q

P

A

L
|
L

Y
|
Y

R

E

E

K

C

N

A

A

H

S

V

F

V

I

L

I

D

D

A

A

binding 7-amino-4-hydroxy-3-[(E)-(5-hydroxy-7-sulfonaphthalen-2-yl)diazenyl]naphthalene-2-sulfonic acid: F9 (≠ P16), R45 (≠ E52), F48 (≠ V55), R57 (= R64), G80 (= G86), G81 (= G87), M84 (≠ L90), R116 (= R127), R132 (= R146), L135 (= L149), Y136 (= Y150)

3mufA Shikimate kinase from helicobacter pylori in complex with shikimate-3- phosphate and adp (see paper)
29% identity, 86% coverage: 9:160/177 of query aligns to 1:145/160 of 3mufA

query
sites
3mufA

R

Q

R

H

V

L

Y

V

L

L

V

I

G

G

P

F

R
x
M

A
x
G

S
|
S

G
|
G

K
|
K

T
x
S

V

L

G

A

K

Q

A

E

L

L

A

G

A

L

R

A

A

L

G

K

W

L

H

E

F

V

E

L

D

D

T

T

D
|
D

A

M

T

I

V

I

A

S

A

E

L

R

A

V

G

G

M

L

T

S

V

V

E

R

Q

E

I

I

V

F

E

E

R

E

E

L

G

G

W

E

E

D

G

N

F

F

R
|
R

R

M

R

F

E

E

T

K

E

N

A

L

L

I

R

D

-

E

-

L

A

K

T

T

L

L

G

K

H

T

D

P

G

H

L

-

V

V

V

I

A

S

T

T

G
|
G

M

I

V

V

L

M

A

H

E

E

E

N

N

-

R

-

M

-

L

L

R

K

D

G

G

L

G

G

L

T

V

T

A

F

Y

Y

L

L

C

K

G
x
M

S

D

V

F

D

E

L

T

L

L

A

I

G

K

R
|
R

L

L

A

N

R

Q

D

K

P

E

L

-

A

R

A

E

Q

K

R
|
R

P

P

S

L

L

L

T

N

G

N

R

L

P

T

I

Q

A

A

D

K

E

E

V

L

A

F

E

E

V

-

L

-

A

-

A

K

R
|
R

E

Q

P

A

L

L

Y

Y

R

E

E

K

C

N

A

A

H

S

V

F

V

I

L

I

D

D

A

A

binding adenosine-5'-diphosphate: G10 (≠ A18), S11 (= S19), G12 (= G20), K13 (= K21), S14 (≠ T22), S15 (≠ T23), M98 (≠ G109), R106 (= R117)
binding shikimate-3-phosphate: M9 (≠ R17), D32 (= D40), R56 (= R64), G78 (= G85), G79 (= G86), G80 (= G87), R115 (= R127), R131 (= R146)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 147, 148

1zuiA Structural basis for shikimate-binding specificity of helicobacter pylori shikimate kinase (see paper)
29% identity, 86% coverage: 9:160/177 of query aligns to 1:143/158 of 1zuiA

query
sites
1zuiA

R

Q

R

H

V

L

Y

V

L

L

V

I

G

G

P

F

R
x
M

A

G

S

S

G

G

K

K

T

S

V

L

G

A

K

Q

A

E

L

L

A

G

A

L

R

A

A

L

G

K

W

L

H

E

F

V

E

L

D

D

T

T

D
|
D

A

M

T

I

V

I

A

S

A

E

L

R

A

V

G

G

M

L

T

S

V

V

E

R

Q

E

I

I

V
x
F

E

E

R

E

E

L

G

G

W

E

E

D

G

N

F

F

R
|
R

R

M

R

F

E

E

T

K

E

N

A

L

L

I

R

D

-

E

-

L

A

K

T

T

L

L

G

K

H

T

D

P

G

H

L

-

V

V

V

I

A

S

T

T

G
|
G

G

G

M

I

V

V

L

M

A

H

E

E

E

N

N

-

R

-

M

-

L

L

R

K

D

G

G

L

G

G

L

T

V

T

A

F

Y

Y

L

L

C

K

G

M

S

D

V

F

D

E

L

T

L

L

A

I

G

K

R

R

L

L

A

N

R

Q

D

-

P

-

L

-

A

R

A
x
E

Q

K

R
|
R

P

P

S

L

L

L

T

N

G

N

R

L

P

T

I

Q

A

A

D

K

E

E

V

L

A

F

E

E

V

-

L

-

A

-

A

K

R
|
R

E

Q

P

A

L

L

Y

Y

R

E

E

K

C

N

A

A

H

S

V

F

V

I

L

I

D

D

A

A

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: M9 (≠ R17), D32 (= D40), F47 (≠ V55), R56 (= R64), G78 (= G85), G79 (= G86), E111 (≠ A125), R113 (= R127), R129 (= R146)

Query Sequence

>8500662 FitnessBrowser__Miya:8500662
MNDGKRQARRVYLVGPRASGKTTVGKALAARAGWHFEDTDATVAALAGMTVEQIVEREGW
EGFRRRETEALRATLGHDGLVVATGGGMVLAEENRRMLRDGGLVAYLCGSVDLLAGRLAR
DPLAAQRPSLTGRPIADEVAEVLAAREPLYRECAHVVLDAGDDLRVLVERLASLLEG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory