SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 8501132 FitnessBrowser__Miya:8501132 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 5 hits to proteins with known functional sites (download)

5odqB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with bromoethanesulfonate. (see paper)
28% identity, 33% coverage: 474:712/716 of query aligns to 1:265/291 of 5odqB

query
sites
5odqB

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binding Non-cubane [4Fe-4S]-cluster: G8 (= G481), C9 (= C482), C41 (= C514), C42 (= C515), C78 (= C551), G80 (≠ S553), C81 (= C554), G152 (≠ S613), C153 (= C614), H154 (= H615), C193 (= C646), C194 (= C647), C231 (= C684), F233 (≠ G686), C234 (= C687)
binding 2-bromanylethanesulfonic acid: G46 (≠ V519), G80 (≠ S553), H154 (= H615), G197 (= G650), R201 (≠ S654), F233 (≠ G686), L236 (≠ M689)

5odhH Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with heterodisulfide for 3.5 minutes (see paper)
28% identity, 33% coverage: 474:712/716 of query aligns to 1:265/291 of 5odhH

query
sites
5odhH

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binding Non-cubane [4Fe-4S]-cluster: G8 (= G481), C9 (= C482), C41 (= C514), C42 (= C515), C78 (= C551), G80 (≠ S553), C81 (= C554), C153 (= C614), H154 (= H615), C193 (= C646), C194 (= C647), C231 (= C684), F233 (≠ G686), C234 (= C687)
binding 1-thioethanesulfonic acid: A44 (≠ L517), P45 (= P518), G46 (≠ V519), G80 (≠ S553), H154 (= H615), G197 (= G650), G198 (= G651)
binding Coenzyme B: R201 (≠ S654), F233 (≠ G686), R240 (≠ G693)

5odhB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with heterodisulfide for 3.5 minutes (see paper)
28% identity, 33% coverage: 474:712/716 of query aligns to 1:265/291 of 5odhB

query
sites
5odhB

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binding Non-cubane [4Fe-4S]-cluster: G8 (= G481), C9 (= C482), I10 (≠ V483), C41 (= C514), C42 (= C515), C78 (= C551), G80 (≠ S553), C81 (= C554), G152 (≠ S613), C153 (= C614), H154 (= H615), C193 (= C646), C194 (= C647), C231 (= C684), F233 (≠ G686), C234 (= C687)
binding 1-thioethanesulfonic acid: P45 (= P518), G46 (≠ V519), G80 (≠ S553), F233 (≠ G686)

5odcB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus at 2.3 a resolution (see paper)
28% identity, 33% coverage: 474:712/716 of query aligns to 1:265/291 of 5odcB

query
sites
5odcB

L

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binding Non-cubane [4Fe-4S]-cluster: G8 (= G481), C9 (= C482), I10 (≠ V483), C41 (= C514), C42 (= C515), C78 (= C551), N79 (≠ A552), G80 (≠ S553), C81 (= C554), G152 (≠ S613), C153 (= C614), C193 (= C646), C194 (= C647), G197 (= G650), C231 (= C684), F233 (≠ G686), C234 (= C687)

7bkbB Formate dehydrogenase - heterodisulfide reductase - formylmethanofuran dehydrogenase complex from methanospirillum hungatei (hexameric, composite structure) (see paper)
25% identity, 33% coverage: 477:712/716 of query aligns to 5:269/296 of 7bkbB

query
sites
7bkbB

A

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I

I

L

A

T

L

L

I

C
|
C

A

N

S

G

C
|
C

A

Y

S

K

H

S

L

I

K

W

E

E

G

-

Y

V

P

N

N

H

I

I

L

L

A

K

G

H

Q

N

T

D

D

E

M

L

T

R

G

D

K

N

V

V

-

N

K

E

L

V

F

L

A

A

S

E

K

I

V

D

I

M

D

Q

F

F

S

K

S

G

F

T

V

I

H

-

D

D

V

V

L

W

G

H

M

L

T

A

A

E

D

L

D

Y

F

Y

K

D

G

D

K

K

-

V

-

C

-

G

-

V

-

Q

-

K

-

I

-

K

-

D

-

S

-

V

-

T

-

P

-

L

-

S

G

G

E

A

K

K

V

V

A

A

Y

A

H

H

S

Y

S
x
G

C
|
C

H

H

L

L

C

M

-

K

-

P

-

K

-

E

R

R

G

H

L

F

G

G

V

D

V

T

E

E

Q

N

P

P

-

M

-

W

-

F

R

E

A

E

L

L

I

I

A

G

S

A

S

L

G

G

S

A

E

E

Y

P

C

I

P

Q

A

Y

Q

R

E

N

E

K

A

M

V

Q

C
|
C

G

G

F

A

G

G

T

G

F

V

S

R

M

G

K

Y

F

D

P

I

E

V

L

H

S

A

K

L

E

D

L

I

L

T

D

N

K

E

K

K

L

L

N

I

N

N

A

I

E

Q

A

E

T

A

G

G

A

A

T

D

R

A

M

I

V

T

A

E

D

L

C
|
C

P

P

G
x
F

C
|
C

I

Q

M

L

Q

Q

I

F

R

D

G

R

G

G

A

Q

-

I

-

E

-

I

-

K

E

E

K

K

R

F

G

G

S

D

-

V

-

Y

R

N

M

I

K

P

V

V

G

L

H

H

I

Y

A

N

E

E

L

L

A

G

binding Non-cubane [4Fe-4S]-cluster: G9 (= G481), C10 (= C482), C42 (= C514), C43 (= C515), C79 (= C551), C82 (= C554), G156 (≠ S613), C157 (= C614), C197 (= C646), C198 (= C647), C235 (= C684), F237 (≠ G686), F237 (≠ G686), C238 (= C687)

Query Sequence

>8501132 FitnessBrowser__Miya:8501132
MQTAKTLKEYRKELRESLDNEFLREAMDKFATAYPVSRANAFRGLDEKAMIAEVADAKDA
AAKNMDALYAQFKAEAKKRGVKVHMAKDAAEANEIIARIAKNSNCKKIVKSKSMTAEETL
LNHRLEEDGLEVIETDLGEWIIQLRHEGPTHMVMPAIHLSRYQVADLFSEVTKQKQEVDI
QRLVKVARRELRQHFATADMGISGANFAIAESGTIGLVTNEGNARLVTTLPRVHVALCGL
DKLTPSLNDALKSLRVLPRNATGQAITSYVTWITGANECKAAADEKKEMHIVFLDNGRRA
LAQDPLFSQVMRCVRCGACANVCPVYRLVGGHKMGHIYIGAIGLILTYFFHGKDKARNLV
QNCINCESCKSICAGGIDLPRLIKEIRARLSEEDGAPVEATLLGKVLKNRKLFHTLLRFG
KFAQKPVTGGTPYLRHLPQMFMKDHGFRALPAIADKAFRDEWETIRPRVSNAKLRVALFS
GCVQDFVYPEQMKAAVKVLASRGVEMDFPMDQSCCGLPVQMMAERQATIDVARQNVMAFD
AAKYDYILTLCASCASHLKEGYPNILAGQTDMTGKVKLFASKVIDFSSFVHDVLGMTADD
FKGKGEKVAYHSSCHLCRGLGVVEQPRALIASSGSEYCPAQEEAVCCGFGGTFSMKFPEL
SKELLDKKLNNAEATGATRMVADCPGCIMQIRGGAEKRGSRMKVGHIAELLAENLK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory