SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 8501181 FitnessBrowser__Miya:8501181 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

B0SR19 Sugar transporter SemiSWEET from Leptospira biflexa serovar Patoc (strain Patoc 1 / ATCC 23582 / Paris) (see paper)
38% identity, 84% coverage: 16:97/98 of query aligns to 3:84/85 of B0SR19

query
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B0SR19

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W48 (= W61) mutation to A: Impairs glucose transport.
N64 (= N77) mutation to A: Impairs glucose transport.

5uhsA Structure of a semisweet d57a mutant (see paper)
39% identity, 79% coverage: 16:92/98 of query aligns to 3:79/81 of 5uhsA

query
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5uhsA

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binding beta-D-glucopyranose: F41 (≠ L54), W48 (= W61)

Query Sequence

>8501181 FitnessBrowser__Miya:8501181
MPAPTADSAALSTLFEIIGYAAGLLTSLAYLPQVVRIARTRSADDISLPTFRLLAVGVAL
WLVYGIGIGSWPVMAANAVGLALILAVIWLKLRYSRQL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory