SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 8501230 FitnessBrowser__Miya:8501230 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

6j2lA Crystal structure of bi-functional enzyme (see paper)
47% identity, 72% coverage: 14:109/133 of query aligns to 10:104/200 of 6j2lA

query
sites
6j2lA

D

D

F

W

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E

K

K

M

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D

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G

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binding zinc ion: D79 (= D83), D81 (= D85), D83 (= D87), C96 (= C101), C103 (= C108)

Sites not aligning to the query:

binding zinc ion: 152, 171, 174

6j2lB Crystal structure of bi-functional enzyme (see paper)
45% identity, 66% coverage: 14:101/133 of query aligns to 9:96/185 of 6j2lB

query
sites
6j2lB

D

D

F

W

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E

K

K

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D
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D

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C

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C

binding zinc ion: D78 (= D83), D80 (= D85), D82 (= D87)

Sites not aligning to the query:

binding magnesium ion: 137, 140, 156, 159

7bgnA Crystal structure of mthisn2-amp complex, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
41% identity, 72% coverage: 20:115/133 of query aligns to 15:109/204 of 7bgnA

query
sites
7bgnA

G

G

L

L

V

A

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V

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A

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D

binding adenosine monophosphate: W60 (= W65), K62 (= K67), G63 (= G68), T65 (= T70), S66 (= S71), T94 (≠ A100), H96 (= H102)
binding zinc ion: D78 (= D83), D80 (= D85), D82 (= D87), C95 (= C101), C102 (= C108)

Sites not aligning to the query:

binding adenosine monophosphate: 121, 128, 135, 136, 137, 180, 203, 204
binding zinc ion: 154, 174, 177

7bgmA Crystal structure of mthisn2, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
42% identity, 68% coverage: 20:110/133 of query aligns to 17:106/213 of 7bgmA

query
sites
7bgmA

G

G

L

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binding zinc ion: D80 (= D83), D82 (= D85), D84 (= D87), C97 (= C101), C104 (= C108)

Sites not aligning to the query:

binding zinc ion: 163, 183, 186

Query Sequence

>8501230 FitnessBrowser__Miya:8501230
MAQASDAAQGFRPDFAKMNGLVPAIAQCAATGEVLMMAWMNEEAWDATLATGEAHYFSRS
RGRLWHKGGTSGHTQHIKAVRLDCDSDTVLLLVDQKGDAACHEGYRSCFYRELKDGAPVI
CSPRVFDPKEVYK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory