SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 8501604 DvMF_2322 bifunctional acetaldehyde-CoA/alcohol dehydrogenase (RefSeq) to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7bvpA Adhe spirosome in extended conformation (see paper)
60% identity, 95% coverage: 32:889/904 of query aligns to 5:864/869 of 7bvpA

query
sites
7bvpA

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binding nicotinamide-adenine-dinucleotide: I110 (= I137), V111 (≠ I138), P112 (= P139), T113 (= T140), T114 (= T141), H139 (= H166), T193 (= T220), G194 (= G221), G195 (= G222), G197 (= G224), M198 (= M225), A201 (= A228), V212 (= V239), G213 (= G240), A214 (= A241), C246 (= C273), E335 (= E362), L337 (= L364), H367 (= H394), L417 (= L443), T418 (= T444), L419 (= L445), D487 (= D511), F489 (≠ T513), A518 (≠ P542), D519 (= D543), P520 (= P544), G546 (= G570), S547 (= S571), D550 (= D574), T597 (= T621), T598 (= T622), T601 (= T625), S603 (= S627), F608 (= F632), V610 (= V634), K619 (= K643), M642 (= M666), L646 (= L670), F714 (= F738), H737 (= H761)
binding zinc ion: D653 (= D677), H657 (= H681), H723 (= H747), H737 (= H761)

6tqmA Escherichia coli adhe structure in its compact conformation (see paper)
60% identity, 95% coverage: 32:889/904 of query aligns to 5:864/869 of 6tqmA

query
sites
6tqmA

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binding fe (iii) ion: D653 (= D677), H657 (= H681), H723 (= H747), H737 (= H761)
binding [[(2R,3S,4R,5R)-5-[(3R)-3-aminocarbonyl-3,4-dihydro-2H-pyridin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanidyl-phosphoryl] [(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl phosphate: D487 (= D511), L490 (≠ M514), G545 (= G569), G546 (= G570), S547 (= S571), D550 (= D574), T597 (= T621), S603 (= S627), F608 (= F632), A609 (= A633), V610 (= V634), L646 (= L670), H727 (= H751), H737 (= H761)

P0A9Q7 Bifunctional aldehyde-alcohol dehydrogenase AdhE; Alcohol dehydrogenase E; EC 1.2.1.10; EC 1.1.1.1 from Escherichia coli (strain K12) (see 8 papers)
60% identity, 95% coverage: 32:889/904 of query aligns to 5:864/891 of P0A9Q7

query
sites
P0A9Q7

N

N

S

V

I

A

T

E

V

L

D

N

D

A

I

L

V

V

T

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E

E

A

A

G

G

I

V

A

Q

E

E

A

A

D

D

F

F

L

L

E

A

R

N

V

V

D

D

L

K

L

L

A

S

E

E

Q

D

A

A

F

F

D

D

D

D

Q

Q

C

C

T

T

G

G

G

A

N

N

P

P

R

R

Y

Y

P

P

L

L

I

I

A

S

E

E

I

L

R

K

E

Q

L

I

Y

L

L

L

K

D

A

T

Y

Y

Y

Y

G

G

IVPTTN 110:115 (≠ IIPTTN 137:142) binding
G195 (= G222) binding
G213 (= G240) binding
A267 (= A294) mutation to T: Shows aerobic growth ability on ethanol. Shows 5-6 fold increase in acetaldehyde dehydrogenase activity, but does not affect ethanol dehydrogenase activity. Shows decreased thermal enzyme stability and increased sensitivity to MCO damage. Shows increased protein stability and resistance to MCO; when associated with K-568.
E335 (= E362) binding
K358 (≠ R385) modified: N6-acetyllysine
L419 (= L445) binding
KRAE 446:449 (≠ ERRE 472:475) mutation Missing: Can form dimers, but does not assemble into long filaments. Strongly affects ALDH activity, but not ADH activity.
D487 (= D511) binding
D519 (= D543) binding
GSPMD 546:550 (= GSPMD 570:574) binding
E568 (= E592) mutation to K: Partially restores protein stability and resistance to MCO damage; when associated with T-267.
V610 (= V634) binding
K619 (= K643) binding
D653 (= D677) binding
H657 (= H681) binding
F670 (= F694) mutation F->A,E,V: Disrupts spirosome formation. Affects the forward activity of ALDH.
H723 (= H747) binding
H737 (= H761) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P0A9Q8 Bifunctional aldehyde-alcohol dehydrogenase AdhE; Alcohol dehydrogenase E; EC 1.2.1.10; EC 1.1.1.1 from Escherichia coli O157:H7 (see paper)
60% identity, 95% coverage: 32:889/904 of query aligns to 5:864/891 of P0A9Q8

query
sites
P0A9Q8

N

N

S

V

I

A

T

E

V

L

D

N

D

A

I

L

V

V

T

E

R

R

V

V

N

K

E

K

A

A

Q

Q

R

R

A

E

F

Y

A

A

N

S

F

F

T

T

Q

Q

Q

E

Q

Q

V

V

D

D

A

K

I

I

F

F

H

R

A

A

A

A

A

A

A

L

A

A

A

A

T

A

A

D

Q

A

R

R

I

I

H

P

L

L

A

A

R

K

M

M

A

A

V

V

Q

A

E

E

T

S

G

G

M

M

G

G

I

I

L

V

E

E

D

D

K

K

V

V

I

I

K

K

N

N

H

H

F

F

A

A

S

S

E

E

Y

Y

I

I

Y

Y

N

N

K

A

Y

Y

K

K

D

D

D

E

K

K

T

T

C

C

G

G

V

V

I

L

R

S

D

E

D

D

P

D

A

T

Y

F

G

G

Y

T

R

I

E

T

V

I

A

A

A

E

P

P

I

I

G

G

V

I

I

I

A

C

G

G

I

I

I

V

P

P

T

T

T

T

N

N

P

P

T

T

S

S

T

T

T

A

I

I

F

F

K

K

A

S

L

L

L

I

A

S

L

L

K

K

T

T

R

R

N

N

G

A

I

I

I

I

F

F

A

S

P

P

H

H

P

P

R

R

A

A

A

K

K

D

S

A

T

T

V

N

E

K

A

A

A

A

R

D

I

I

V

V

H

L

E

Q

A

A

A

A

V

I

A

A

A

A

G

G

A

A

P

P

R

K

G

D

I

L

I

I

G

G

W

W

V

I

E

D

A

Q

P

P

T

S

P

V

D

E

L

L

T

S

R

N

Q

A

L

L

M

M

Q

H

H

H

R

P

G

D

V

I

A

N

L

L

I

I

L

L

A

A

T

T

G

G

G

G

P

P

G

G

M

M

V

V

H

K

A

A

A

A

Y

Y

S

S

S

S

G

G

K

K

P

P

A

A

I

I

G

G

V

V

G

G

A

A

G

G

N

N

T

T

P

P

V

V

V

V

V

I

D

D

A

E

S

T

A

A

N

D

V

I

K

K

M

R

A

A

V

V

N

A

S

S

I

V

I

L

L

M

S

S

K

K

T

T

F

F

D

D

N

N

G

G

M

V

I

I

C

C

A

A

S

S

E

E

Q

Q

A

S

V

V

I

V

V

V

E

V

D

D

A

S

A

V

A

Y

D

D

A

A

V

V

K

R

A

E

E

R

F

F

A

A

A

T

R

H

G

G

C

G

H

Y

F

L

A

L

S

Q

P

G

Q

K

E

E

A

L

E

K

A

A

L

V

A

Q

G

D

V

V

V

I

F

L

T

K

D

N

G

G

R

A

L

L

N

N

A

A

A

A

I

I

V

V

G

G

R

Q

S

P

A

A

A

Y

E

K

I

I

A

A

A

E

M

L

A

A

G

G

I

F

T

S

V

V

P

P

P

E

T

N

T

T

K

K

I

I

L

L

I

I

A

G

E

E

R

V

D

T

A

V

I

V

D

D

P

E

L

S

D

E

P

P

F

F

A

A

H

H

E

E

K

K

L

L

S

S

P

P

V

T

L

L

G

A

F

M

Y

Y

R

R

A

A

P

K

D

D

F

F

A

E

A

D

A

A

V

V

D

E

M

K

A

A

Q

E

R

K

L

L

V

V

E

A

L

M

G

G

G

G

A

I

G

G

H

H

T

T

S

S

V

C

L

L

Y

Y

T

T

N

D

E

Q

A

D

N

N

R

Q

-

P

E

A

R

R

I

V

V

S

H

Y

F

F

Q

G

N

Q

V

K

L

M

T

K

T

T

G

A

R

R

T

I

L

L

V

I

N

N

M

T

P

P

S

A

S

S

Q

Q

G

G

A

G

I

I

G

G

D

D

V

L

Y

Y

N

N

F

F

E

K

L

L

A

A

P

P

S

S

L

L

T

T

L

L

G

G

C

C

G

G

S

S

W

W

G

G

D

G

N

N

S

S

V

I

S

S

E

E

N

N

I

V

G

G

V

P

K

K

H

H

L

L

M

I

N

N

V

K

K

K

T

T

V

V

A

A

E

K

R

R

R

A

E

E

N

N

M

M

L

L

W

W

F

H

R

K

V

L

P

P

P

K

K

S

I

I

Y

Y

F

F

K

R

M

R

G

G

A

S

L

L

R

P

L

I

A

A

L

L

E

D

D

E

M

V

-

I

-

T

R

D

D

G

R

H

K

K

R

R

A

A

F

L

I

I

V

V

T

T

D
|
D

R

R

T

F

M

L

E

F

D

N

L

N

G

G

H

Y

V

A

G

D

K

Q

V

I

T

T

A

S

V

V

L

L

E

K

K

A

L

A

G

G

I

V

Q

E

F

T

R

E

V

V

F

F

S

F

D

E

V

V

K

E

P

A

D
|
D

P

P

D

T

L

L

S

S

G

I

T

V

Y

R

A

K

A

G

L

A

D

E

S

L

I

A

R

N

A

S

F

F

R

K

P

P

D

D

M

V

F

I

I

I

A

A

L

L

G

G

G

G

G
|
G

S
|
S

P
|
P

M
|
M

D
|
D

A

A

A

A

K

K

I

I

M

M

W

W

L

V

M

M

Y

Y

E

E

Q

H

P

P

D

E

L

T

K

H

F

F

E

E

E

E

I

L

S

A

L

L

R

R

F

F

M

M

D

D

I

I

R

R

K

K

R

R

V

I

H

Y

A

K

F

F

P

P

A

K

L

M

G

G

K

V

K

K

A

A

V

K

M

M

V

I

A

A

V

V

P

T

T
|
T

T
|
T

S

S

G

G

T

T

G

G

S

S

E

E

V

V

T

T

P

P

F

F

A

A

V

V

I

V

T

T

D

D

D

D

A

A

T

T

G

G

M

Q

K

K

Y

Y

P

P

I

L

A

A

D

D

Y

Y

E

A

L

L

T

T

P

P

D

D

M

M

A

A

I

I

V

V

D

D

P

A

E

N

F
x
L

V

V

M

M

D

D

M

M

P

P

K

K

T

S

L

L

T

C

A

A

H

F

S

G

G

G

L

L

D
|
D

A

A

L

V

T

T

H
|
H

A

A

V

M

E

E

A

A

F

Y

T

V

S

S

T

V

Y

L

A

A

N

S

N

E

F

F

S

S

D

D

G

G

N

Q

A

A

L

L

E

Q

A

A

V

L

R

K

L

L

V

L

F

K

K

E

Y

Y

L

L

R

P

R

A

A

S

Y

Y

N

H

D

E

G

G

A

S

R

K

D

N

V

P

M

V

A

A

R

R

E

E

K

R

M

V

H

H

Y

S

A

A

G

A

T

T

I

I

A

A

G

G

M

I

A

A

F

F

A

A

N

N

A

A

F

F

L

L

G

G

V

V

C

C

H
|
H

S

S

M

M

A

A

H

H

K

K

L

L

G

G

A

S

A

Q

F

F

H

H

M

I

P

P

H
|
H

G

G

L

L

A

A

N

N

A

A

L

L

L

L

L

I

S

C

H

N

V

V

I

I

E

R

Y

Y

N

N

A

A

T

N

D

D

T

N

P

P

T

T

K

K

Q

Q

G

T

L

A

M

F

P

S

Q

Q

Y

Y

R

D

Y

R

P

P

F

Q

V

A

K

R

G

R

R

R

Y

Y

A

A

R

E

I

I

A

A

D

D

M

H

L

L

G

G

L

L

T

S

E

-

G

A

C

P

G

G

D

D

D

R

R

T

D

A

R

A

K

K

V

I

A

E

R

K

L

L

V

L

Q

A

A

W

I

L

E

E

Q

T

L

L

K

K

A

A

D

E

L

L

N

G

V

I

P

P

G

K

S

S

L

I

R

R

E

E

A

A

G

G

I

V

A

Q

E

E

A

A

D

D

F

F

L

L

E

A

R

N

V

V

D

D

L

K

L

L

A

S

E

E

Q

D

A

A

F

F

D

D

D

D

Q

Q

C

C

T

T

G

G

G

A

N

N

P

P

R

R

Y

Y

P

P

L

L

I

I

A

S

E

E

I

L

R

K

E

Q

L

I

Y

L

L

L

K

D

A

T

Y

Y

Y

Y

G

G

D487 (= D511) binding
D519 (= D543) binding
GSPMD 546:550 (= GSPMD 570:574) binding
TT 597:598 (= TT 621:622) binding
L638 (≠ F662) binding
D653 (= D677) binding
H657 (= H681) binding
H723 (= H747) binding
H737 (= H761) binding

6scgA Structure of adhe form 1 (see paper)
57% identity, 46% coverage: 476:889/904 of query aligns to 1:401/406 of 6scgA

query
sites
6scgA

N

D

M

M

L

L

W

W

F

H

R

K

V

L

P

P

P

K

K

S

I

I

Y

Y

F

F

K

R

M

R

G

G

A

S

L

L

R

P

L

I

A

A

L

L

E

D

D

E

M

V

-

I

-

T

R

D

D

G

R

H

K

K

R

R

A

A

F

L

I

I

V

V

T

T

D
|
D

R

R

T
x
F

M
x
L

E

F

D

N

L

N

G

G

H

Y

V

A

G

D

K

Q

V

I

T

T

A

S

V

V

L

L

E

K

K

A

L

A

G

G

I

V

Q

E

F

T

R

E

V

V

F

F

S

F

D

E

V

V

K

E

P
x
A

D
|
D

P

P

D

T

L

L

S

S

G

I

T

V

Y

R

A

K

A

G

L

A

D

E

S

L

I

A

R

N

A

S

F

F

R

K

P

P

D

D

M

V

F

I

I

I

A

A

L

L

G

G

G
|
G

G
|
G

S
|
S

P

P

M

M

D
|
D

A

A

A

A

K

K

I

I

M

M

W

W

L

V

M

M

Y

Y

E

E

Q

H

P

P

D

E

L

T

K

H

F

F

E

E

E

E

I

L

S

A

L

L

R

R

F

F

M

M

D

D

I

I

R

R

K

K

R

R

V

I

H

Y

A

K

F

F

P

P

A

K

L

M

G

G

K

V

K

K

A

A

V

K

M

M

V

I

A

A

V

V

P

T

T
|
T

T
|
T

S

S

G

G

T
|
T

G

G

S

S

E

E

V

V

T

T

P

P

F

F

A

A

V
|
V

I

V

T

T

D

D

D

D

A

A

T

T

G

G

M

Q

K

K

Y

Y

P

P

I

L

A

A

D

D

Y

Y

E

A

L

L

T

T

P

P

D

D

M

M

A

A

I

I

V

V

D

D

P

A

E

N

F
x
L

V

V

M

M

D

D

M
|
M

P
|
P

K

K

T

S

L
|
L

T

C

A

A

H

F

S

G

G

G

L

L

D
|
D

A

A

L

V

T

T

H
|
H

A

A

V

M

E

E

A

A

F

Y

T

V

S

S

T

V

Y

L

A

A

N

S

N

E

F

F

S

S

D

D

G

G

N

Q

A

A

L

L

E

Q

A

A

V

L

R

K

L

L

V

L

F

K

K

E

Y

Y

L

L

R

P

R

A

A

S

Y

Y

N

H

D

E

G

G

A

S

R

K

D

N

V

P

M

V

A

A

R

R

E

E

K

R

M

V

H

H

Y

S

A

A

G

A

T

T

I

I

A

A

G

G

M

I

A

A

F

F

A

A

N

N

A

A

F

F

L

L

G

G

V

V

C

C

H
|
H

S

S

M

M

A

A

H
|
H

K

K

L

L

G

G

A

S

A

Q

F

F

H

H

M

I

P

P

H
|
H

G

G

L

L

A

A

N

N

A

A

L

L

L

L

L

I

S

C

H

N

V

V

I

I

E

R

Y

Y

N

N

A

A

T

N

D

-

T

-

P

-

T

-

K

-

Q

-

G

-

L

-

M

-

P

P

Q

Q

Y

A

R

R

Y

R

P

-

F

-

V

-

K

-

G

-

R

R

Y

Y

A

A

R

E

I

I

A

A

D

D

M

H

L

L

G

G

L

L

T

S

E

-

G

A

C

P

G

G

D

D

D

R

R

T

D

A

R

A

K

K

V

I

A

E

R

K

L

L

V

L

Q

A

A

W

I

L

E

E

Q

T

L

L

K

K

A

A

D

E

L

L

N

G

V

I

P

P

G

K

S

S

L

I

R

R

E

E

A

A

G

G

I

V

A

Q

E

E

A

A

D

D

F

F

L

L

E

A

R

N

V

V

D

D

L

K

L

L

A

S

E

E

Q

D

A

A

F

F

D

D

D

D

Q

Q

C

C

T

T

G

G

G

A

N

N

P

P

R

R

Y

Y

P

P

L

L

I

I

A

S

E

E

I

L

R

K

E

Q

L

I

Y

L

L

L

K

D

A

T

Y

Y

Y

Y

G

G

binding fe (iii) ion: D204 (= D677), H208 (= H681), H274 (= H747), H288 (= H761)
binding nicotinamide-adenine-dinucleotide: D38 (= D511), F40 (≠ T513), L41 (≠ M514), A69 (≠ P542), D70 (= D543), G96 (= G569), G97 (= G570), S98 (= S571), D101 (= D574), T148 (= T621), T149 (= T622), T152 (= T625), V161 (= V634), L189 (≠ F662), M193 (= M666), P194 (= P667), L197 (= L670), H278 (= H751)

3zdrA Structure of the alcohol dehydrogenase (adh) domain of a bifunctional adhe dehydrogenase from geobacillus thermoglucosidasius ncimb 11955 (see paper)
54% identity, 46% coverage: 476:889/904 of query aligns to 1:402/403 of 3zdrA

query
sites
3zdrA

N

N

M

M

L

Q

W

W

F

F

R

K

V

V

P

P

P

P

K

K

I

I

Y

Y

F

F

K

E

M

K

G

N

A

A

L

V

R

Q

L

Y

A

-

L

L

E

A

D

K

M

M

R

P

D

D

R

I

K

S

R

R

A

A

F

F

I

I

V

V

T

T

D

D

R

P

T

G

M

M

E

V

D

K

L

L

G

G

H

Y

V

V

G

D

K

K

V

V

T

L

A

Y

V

Y

L

L

E

R

K

R

L

R

G

P

-

D

-

Y

I

V

Q

H

F

S

R

E

V

I

F

F

S

S

D

E

V

V

K

E

P

P

D

D

P

P

D

S

L

I

S

E

G

T

T

V

Y

M

A

K

A

G

L

V

D

D

S

M

I

M

R

R

A

S

F

F

R

E

P

P

D

D

M

V

F

I

I

I

A

A

L

L

G

G

G

G

G

G

S

S

P

P

M

M

D

D

A

A

A

A

K

K

I

A

M

M

W

W

L

L

M

F

Y

Y

E

E

Q

H

P

P

D

T

L

A

K

D

F

F

E

N

E

A

I

L

S

K

L

Q

R

K

F

F

M

L

D

D

I

I

R

R

K

K

R

R

V

V

H

Y

A

K

F

Y

P

P

A

K

L

L

G

G

K

Q

K

K

A

A

V

K

M

F

V

V

A

A

V

I

P

P

T

T

T

T

S

S

G

G

T

T

G

G

S

S

E

E

V

V

T

T

P

S

F

F

A

A

V

V

I

I

T

T

D

D

D

K

A

K

T

T

G

N

M

I

K

K

Y

Y

P

P

I

L

A

A

D

D

Y

Y

E

E

L

L

T

T

P

P

D

D

M

V

A

A

I

I

V

V

D

D

P

P

E

Q

F

F

V

V

M

M

D

T

M

V

P

P

K

K

T

H

L

V

T

T

A

A

H

D

S

T

G

G

L

M

D
|
D

A

V

L

L

T

T

H
|
H

A

A

V

I

E

E

A

A

F

Y

T

V

S

S

T

N

Y

M

A

A

N

N

N

D

F

Y

S

T

D

D

G

G

N

L

A

A

L

M

E

K

A

A

V

I

R

Q

L

L

V

V

F

F

K

E

Y

Y

L

L

R

P

R

R

A

A

Y

Y

N

Q

D

N

G

G

A

A

R

-

D

D

V

E

M

L

A

A

R

R

E

E

K

K

M

M

H

H

Y

N

A

A

G

S

T

T

I

I

A

A

G

G

M

M

A

A

F

F

A

A

N

N

A

A

F

F

L

L

G

G

V

I

C

N

H
|
H

S

S

M

L

A

A

H

H

K

K

L

L

G

G

A

A

A

E

F

F

H

H

M

I

P

P

H
|
H

G

G

L

R

A

A

N

N

A

T

L

I

L

L

L

M

S

P

H

H

V

V

I

I

E

R

Y

Y

N

N

A

A

T

A

D

-

T

K

P

P

T

K

K

K

Q

-

G

-

L

-

M

-

P

-

Q

-

Y

-

R

-

Y

Y

P

F

F

K

V

A

K

D

G

Q

R

R

Y

Y

A

A

R

E

I

I

A

A

D

R

M

M

L

L

G

G

L

L

T

P

E

A

G

R

C

T

G

T

D

E

D

E

R

-

D

-

R

-

K

G

V

V

A

E

R

S

L

L

V

V

Q

Q

A

A

I

I

E

I

Q

K

L

L

K

A

A

K

D

Q

L

L

N

D

V

M

P

P

G

L

S

S

L

I

R

E

E

A

A

C

G

G

I

V

A

S

E

K

A

Q

D

E

F

F

L

E

E

S

R

K

V

V

D

E

L

K

L

L

A

A

E

E

Q

L

A

A

F

F

D

E

D

D

Q

Q

C

C

T

T

G

T

G

A

N

N

P

P

R

K

Y

L

P

P

L

L

I

V

A

S

E

D

I

L

R

V

E

H

L

I

Y

Y

L

R

K

Q

A

A

Y

F

Y

K

G

G

binding zinc ion: D203 (= D677), H207 (= H681), H272 (= H747), H286 (= H761)

5j7iA Crystal structure of a geobacillus thermoglucosidasius acetylating aldehyde dehydrogenase in complex with adp (see paper)
47% identity, 50% coverage: 21:471/904 of query aligns to 2:444/455 of 5j7iA

query
sites
5j7iA

L

L

N

R

D

D

I

I

S

D

P

L

S

Q

S

S

I

I

A

Q

P

E

N

-

S

-

I

-

T

-

V

V

D

R

D

N

I

Y

V

L

T

E

R

E

V

A

N

K

E

A

A

A

Q

Q

R

K

A

I

F

L

A

E

N

K

F

M

T

T

Q

Q

Q

S

Q

E

V

I

D

D

A

K

I

I

F

V

H

E

A

S

A

M

A

A

A

N

A

A

A

A

T

R

A

E

Q

E

R

A

I

G

H

R

L

L

A

A

R

A

M

M

A

A

V

V

Q

E

E

E

T

T

G

G

M

F

G

G

I

N

L

V

E

E

D

D

K

K

V

T

I

L

K

K

N

N

H

L

F

F

A

A

S

A

E

N

Y

D

I

V

Y

Y

N

N

K

S

Y

I

K

K

D

D

D

V

K

K

T

T

C

V

G

G

V

I

I

I

R

R

D

R

D

D

P

E

A

E

Y

N

G

R

Y

V

R

W

E

E

V

I

A

A

A

Q

P

P

I

V

G

G

V

I

I

V

A

A

G

G

I

I

I

I

P

P

T
x
S

T

T

N

N

P

P

T

T

S

S

T

T

T

V

I

I

F

F

K

K

A

A

L

L

L

I

A

A

L

V

K

K

T

A

R

R

N

N

G

A

I

I

I

V

F

F

A

S

P

P

H
|
H

P
|
P

R

S

A

A

A

A

K

K

S

C

T

T

V

A

E

E

A

A

A

A

R

R

I

I

V

M

H

Q

E

E

A

A

A

A

V

E

A

R

A

A

G

G

A

A

P

P

R

K

G

G

I

L

I

I

G

S

W

C

V

I

E

T

A

Q

P

P

T

T

P

M

D

A

L

A

T
|
T

R

N

Q

E

L

L

M

M

Q

K

H

H

R

K

G

L

V

T

A

D

L

V

I

I

L

L

A

A

T

T

G

G

G

G

P

P

G

G

M

L

V

V

H

K

A

A

A

A

Y

Y

S

S

S

S

G

G

K

K

P

P

A

A

I

Y

G

G

V

V

G

G

A

P

G

G

N

N

T

V

P

P

V

V

V

Y

V

I

D

H

A

E

S