SitesBLAST

C45 (≠ V34) mutation to S: Decreased solubility, loss of dimerization and strongly decreased activity.
E149 (≠ A138) mutation to D: Small effect on NAD(+) interaction, but 40% loss of efficiency. Ability to use NADP(+). 70% loss of efficiency with NAD(+); when associated with V-200. Impaired affinity for both cofactors and decreased catalytic efficiency; when associated with G-200.; mutation to N: Ability to use NADP(+) and 33% decreased efficiency with NAD(+). 70% loss of efficiency with NAD(+); when associated with V-200. Impaired affinity for both cofactors and decreased catalytic efficiency; when associated with G-200.; mutation to Q: Loss of specificity for NAD(+) and loss of 25% efficiency. 15% efficiency with NAD(+); when associated with V-200. Impaired affinity for both cofactors and decreased catalytic efficiency; when associated with G-200.; mutation to T: Loss of specificity and increased NADP(+) binding. Decerased catalytic efficiency. Loss of cofactor specificity and same lower efficiency with both; when associated with V-200. Impaired affinity for both cofactors and decreased catalytic efficiency; when associated with G-200. Changed coenzyme preference from NAD(+) to NADP(+), but no effect on the catalytic efficiency; when associated with R-178 and V-200.
V178 (≠ A167) mutation to R: Changed coenzyme preference from NAD(+) to NADP(+), but no effect on the catalytic efficiency; when associated with T-149 and V-200.
I200 (≠ G189) mutation to G: Changed coenzyme preference from NAD(+) to NADP(+), but impaired affinities for both cofactors. No effect on the interaction with the substrate. Impaired affinities for both cofactors and decreased catalytic efficiencies; when associated with D-149, Q-149, N-149 or T-149.; mutation to V: Also able to use NADP(+) as coenzyme, but no effect on the interaction with the substrate. 15% efficiency with NAD(+); when associated with Q-149. 70% loss of efficiency with NAD(+); when associated with D-149 or N-149. Loss of cofactor specificity and same lower efficiency with both; when associated with T-149. Changed coenzyme preference from NAD(+) to NADP(+), but no effect on the catalytic efficiency; when associated with T-149 and R-178.
C247 (≠ L235) mutation to S: No effect on solubility, but 10% loss of activity.
C253 (≠ R241) mutation to S: No effect on solubility, but loss of activity.

P51648 Aldehyde dehydrogenase family 3 member A2; Aldehyde dehydrogenase 10; Fatty aldehyde dehydrogenase; Microsomal aldehyde dehydrogenase; EC 1.2.1.3; EC 1.2.1.94 from Homo sapiens (Human) (see 5 papers)
31% identity, 98% coverage: 8:460/460 of query aligns to 9:461/485 of P51648

query
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P51648

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I45 (= I45) to F: in SLS; severe loss of activity
V64 (= V64) to D: in SLS; severe loss of activity; dbSNP:rs72547556
L106 (≠ V106) to R: in SLS; severe loss of activity; dbSNP:rs72547558
N112 (≠ A112) mutation to A: Loss of enzyme activity.
P114 (= P114) to L: in SLS; severe loss of activity; dbSNP:rs72547559
P121 (= P121) to L: in SLS; severe loss of activity; dbSNP:rs72547560
T184 (≠ D184) to M: in SLS; severe loss of activity; dbSNP:rs72547562; to R: in SLS; severe loss of activity
G185 (= G185) to A: in SLS; severe loss of activity; dbSNP:rs72547563
E207 (≠ I207) active site; mutation to Q: Loss of enzyme activity.
C214 (≠ A214) to Y: in SLS; 4% of activity; dbSNP:rs72547564
R228 (= R228) to C: in SLS; severe loss of activity; dbSNP:rs72547566
C237 (≠ A237) to Y: in SLS; severe loss of activity; dbSNP:rs72547567
C241 (≠ R241) active site; mutation to S: Loss of enzyme activity.
D245 (= D245) to N: in SLS; severe loss of activity; dbSNP:rs72547568
K266 (≠ E266) to N: in SLS; mild reduction of activity; the underlying nucleotide substitution affects transcript stability; dbSNP:rs72547569
Y279 (≠ F279) to N: in SLS; severe loss of activity; dbSNP:rs72547570
AP 314:315 (≠ PP 320:321) natural variant: AP -> GAKSTVGA (in SLS; 8% of activity)
P315 (= P321) to S: in SLS; common mutation in Europeans; severe loss of enzymatic activity; dbSNP:rs72547571
E331 (= E337) mutation to Q: Loss of enzyme activity.
S365 (≠ T371) to L: in SLS; severe loss of activity; dbSNP:rs72547573
Y410 (≠ M416) mutation to F: Decreased enzyme activity with dodecanal and hexadecanal. No effect on enzyme activity with octanal.
H411 (≠ A417) to Y: in SLS; severe loss of activity
S415 (≠ G421) to N: in SLS; severe loss of activity
F419 (= F425) to S: in SLS; severe loss of activity; dbSNP:rs72547576
R423 (= R429) to H: in SLS; severe loss of activity; dbSNP:rs768290318
K447 (= K451) to E: in SLS; severe loss of activity; dbSNP:rs67939114

Sites not aligning to the query:

445:485 mutation Missing: Decreased enzyme activity with dodecanal. Strongly decreased enzyme activity with hexadecanal. No effect on enzyme activity with octanal.

4l2oA Crystal structure of human aldh3a1 with its selective inhibitor 1-(4- fluorophenyl)sulfonyl-2-methylbenzimidazole
33% identity, 93% coverage: 7:433/460 of query aligns to 9:429/446 of 4l2oA

query
sites
4l2oA

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active site: N114 (≠ A112), K137 (≠ R135), E209 (≠ I207), C243 (≠ R241), E333 (= E337), Y412 (≠ M416)
binding 1-[(4-fluorophenyl)sulfonyl]-2-methyl-1H-benzimidazole: E61 (≠ R59), E62 (= E60), Y65 (≠ P63), Y115 (≠ D113), N118 (≠ R116), L119 (≠ S117), M237 (≠ L235), C243 (≠ R241), I391 (≠ A395), I394 (≠ L398), T395 (≠ A399), F401 (≠ Q405), H413 (≠ A417)
binding nicotinamide-adenine-dinucleotide: T112 (≠ H110), W113 (= W111), N114 (≠ A112), L119 (≠ S117), E140 (≠ A138), L141 (≠ E139), V169 (≠ A167), T172 (≠ D170), T186 (≠ D184), G187 (= G185), S188 (≠ D186), V191 (≠ G189), I194 (≠ T192), E209 (≠ I207), L210 (≠ T208), G211 (= G209), C243 (≠ R241), H289 (≠ G287), E333 (= E337), F335 (= F339), L361 (≠ T365), F401 (≠ Q405)

4h80A Crystal structure of human aldh3a1 with its isozyme selective inhibitor - n-[4-(4-methylsulfonyl-2-nitroanilino)phenyl]acetamide
33% identity, 93% coverage: 7:433/460 of query aligns to 9:429/446 of 4h80A

query
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4h80A

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V

Q

P

P

L

M

A

V

D

G

A

A

I

I

G

A

A

A

G

G

N

N

A

A

V

V

L

L

R
x
K

P

P

S

S

A

E

E

L

A

S

P

E

A

N

T

M

A

A

E

S

M

L

V

L

T

A

R

T

M

I

V

I

R

P

Q

Q

Y

Y

F

L

E

D

P

K

E

D

H

L

V

Y

A

P

V

V

V

I

G

N

G

G

A

V

E

P

T

E

D

T

E

T

A

E

L

L

A

K

T

E

A

R

P

F

D

D

F

H

V

I

W

L

Y

Y

D

T

G

G

D

S

A

T

R

G

G

V

A

G

R

K

T

I

I

I

A

M

V

T

L

A

A

A

P

K

T

H

L

L

T

T

P

P

Y

V

A

T

A

L

I
x
E

T

L

G

G

P

K

S

S

A

P

A

C

L

Y

V

V

H

D

G

K

D

N

A

C

D

D

M

L

A

D

M

V

A

A

A

C

R

R

I

I

V

A

W
|
W

A

G

K

K

F

F

L
x
M

H

N

A

S

G

G

Q

Q

L
x
T

R
x
C

A
x
V

A

A

P

P

D

D

V

Y

L

I

L

L

V

C

Q

D

R

P

T

S

V

I

L

Q

D

N

R

Q

V

I

L

V

D

E

A

K

L

L

R

K

T

K

E

S

L

L

E

K

R

E

A

F

F

Y

G

G

P

E

Q

D

P

A

R

K

T

K

S

S

A

R

D

D

F

Y

G

G

R

R

M

I

V

I

S

S

A

A

A

R

G

H

F

F

A

Q

R

R

-

V

-

M

-

G

-

L

-

I

Q

E

A

G

E

Q

R

K

L

V

A

A

I

Y

G

G

R

-

A

-

L

-

P

-

F

-

G

G

P

T

G

G

D

D

A

A

N

T

Q

R

P

-

D

-

R

-

A

-

S

-

L

-

Y

Y

V

I

P

A

P

P

T

T

L

I

L

L

T

T

D

D

V

V

P

D

D

P

D

Q

S

S

P

P

V

V

L

M

R

Q

E

E

E
|
E

G

I

F

F

G

G

P

P

V

V

L

L

V

P

V

I

R

V

P

C

Y

V

T

R

R

S

L

L

D

E

E

E

A

A

T

I

A

Q

F

F

L

I

A

N

G

Q

L

R

P

E

A

K

L

P

T

L

A

A

L

L

Y

Y

A

M

F

F

T

S

A

N

H

D

A

K

R

V

G

I

E

K

R

K

L

M

M

I

E

A

N

E

T

T

R

S

A

S

G

G

A

G

V

V

L

A

I

A

N

N

D

D

A

V

A
x
I

T

V

H

H

L
x
I

A
x
T

N

L

P

H

R

S

L

L

P

P

Q
x
F

G

G

V

V

G

G

E

N

T

S

G

G

H

M

G

G

A

S

M
x
Y

A

H

G

G

P

K

A

K

G

S

L

F

A

E

T

T

F

F

S

S

A

H

P

R

R

R

A

S

T

C

A

L

V

V

active site: N114 (≠ A112), K137 (≠ R135), E209 (≠ I207), C243 (≠ R241), E333 (= E337), Y412 (≠ M416)
binding N-(4-{[4-(methylsulfonyl)-2-nitrophenyl]amino}phenyl)acetamide: E61 (≠ R59), E62 (= E60), Y65 (≠ P63), Y115 (≠ D113), N118 (≠ R116), L119 (≠ S117), W233 (= W231), M237 (≠ L235), T242 (≠ L240), C243 (≠ R241), V244 (≠ A242), I391 (≠ A395), I394 (≠ L398), T395 (≠ A399), F401 (≠ Q405)

3szbA Crystal structure of human aldh3a1 modified with the beta-elimination product of aldi-1; 1-phenyl- 2-propen-1-one (see paper)
33% identity, 93% coverage: 7:433/460 of query aligns to 9:429/447 of 3szbA

query
sites
3szbA

R

R

Q

A

K

R

A

A

F

A

I

F

A

S

S

S

G

G

A

R

V

T

L

R

P

P

S

L

G

Q

A

F

R

R

V

I

D

Q

A

Q

L

L

R

E

R

A

L

L

R

Q

E

R

G

L

V

I

Q

Q

G

E

Y

Q

R

E

D

Q

R

E

L

L

A

V

D

G

A

A

I

L

R

A

A

A

D

D

Y

L

G

H

R

K

P

N

E

E

H

W

P

N

F

A

L

Y

V

Y

R
x
E

E

E

V

V

P

Y

V

V

L

L

H

E

E

E

I

I

D

E

W

Y

L

M

I

I

K

Q

A

K

V

L

P

P

G

E

F

W

C

A

G

A

G

D

R

E

R

P

V

V

L

E

P

K

S

T

L

P

G

Q

Q

T

F

Q

K

Q

A

D

R

E

S

L

Y

Y

V

I

R

H

R

S

Q

E

P

P

L

L

G

G

R

V

V

V

V

L

A

V

Y

I

A

G

H

T

W

W

A
x
N

D
x
Y

P

P

F

F

R
x
N

S
x
L

L

T

L

I

V

Q

P

P

L

M

A

V

D

G

A

A

I

I

G

A

A

A

G

G

N

N

A

A

V

V

L

L

R
x
K

P

P

S

S

A

E

E

L

A

S

P

E

A

N

T

M

A

A

E

S

M

L

V

L

T

A

R

T

M

I

V

I

R

P

Q

Q

Y

Y

F

L

E

D

P

K

E

D

H

L

V

Y

A

P

V

V

V

I

G

N

G

G

A

V

E

P

T

E

D

T

E

T

A

E

L

L

A

K

T

E

A

R

P

F

D

D

F

H

V

I

W

L

Y

Y

D

T

G

G

D

S

A

T

R

G

G

V

A

G

R

K

T

I

I

I

A

M

V

T

L

A

A

A

P

K

T

H

L

L

T

T

P

P

Y

V

A

T

A

L

I
x
E

T

L

G

G

P

K

S

S

A

P

A

C

L

Y

V

V

H

D

G

K

D

N

A

C

D

D

M

L

A

D

M

V

A

A

A

C

R

R

I

I

V

A

W

W

A

G

K

K

F

F

L

M

H

N

A

S

G

G

Q

Q

L
x
T

R
x
C

A

V

A

A

P

P

D

D

V

Y

L

I

L

L

V

C

Q

D

R

P

T

S

V

I

L

Q

D

N

R

Q

V

I

L

V

D

E

A

K

L

L

R

K

T

K

E

S

L

L

E

K

R

E

A

F

F

Y

G

G

P

E

Q

D

P

A

R

K

T

K

S

S

A

R

D

D

F

Y

G

G

R

R

M

I

V

I

S

S

A

A

A

R

G

H

F

F

A

Q

R

R

-

V

-

M

-

G

-

L

-

I

Q

E

A

G

E

Q

R

K

L

V

A

A

I

Y

G

G

R

-

A

-

L

-

P

-

F

-

G

G

P

T

G

G

D

D

A

A

N

T

Q

R

P

-

D

-

R

-

A

-

S

-

L

-

Y

Y

V

I

P

A

P

P

T

T

L

I

L

L

T

T

D

D

V

V

P

D

D

P

D

Q

S

S

P

P

V

V

L

M

R

Q

E

E

E
|
E

G

I

F

F

G

G

P

P

V

V

L

L

V

P

V

I

R

V

P

C

Y

V

T

R

R

S

L

L

D

E

E

E

A

A

T

I

A

Q

F

F

L

I

A

N

G

Q

L

R

P

E

A

K

L

P

T

L

A

A

L

L

Y

Y

A

M

F

F

T

S

A

N

H

D

A

K

R

V

G

I

E

K

R

K

L

M

M

I

E

A

N

E

T

T

R

S

A

S

G

G

A

G

V

V

L

A

I

A

N

N

D

D

A

V

A

I

T

V

H

H

L
x
I

A

T

N

L

P

H

R

S

L

L

P

P

Q
x
F

G

G

V

V

G

G

E

N

T

S

G

G

H

M

G

G

A

S

M
x
Y

A

H

G

G

P

K

A

K

G

S

L

F

A

E

T

T

F

F

S

S

A

H

P

R

R

R

A

S

T

C

A

L

V

V

active site: N114 (≠ A112), K137 (≠ R135), E209 (≠ I207), C243 (≠ R241), E333 (= E337), Y412 (≠ M416)
binding 1-phenylpropan-1-one: E61 (≠ R59), Y115 (≠ D113), N118 (≠ R116), L119 (≠ S117), T242 (≠ L240), C243 (≠ R241), I394 (≠ L398), F401 (≠ Q405)

4l1oB Crystal structure of human aldh3a1 with inhibitor 1-{[4-(1,3- benzodioxol-5-ylmethyl)piperazin-1-yl]methyl}-1h-indole-2,3-dione
33% identity, 93% coverage: 7:433/460 of query aligns to 9:429/452 of 4l1oB

query
sites
4l1oB

R

R

Q

A

K

R

A

A

F

A

I

F

A

S

S

S

G

G

A

R

V

T

L

R

P

P

S

L

G

Q

A

F

R

R

V

I

D

Q

A

Q

L

L

R

E

R

A

L

L

R

Q

E

R

G

L

V

I

Q

Q

G

E

Y

Q

R

E

D

Q

R

E

L

L

A

V

D

G

A

A

I

L

R

A

A

A

D

D

Y

L

G

H

R

K

P

N

E

E

H

W

P

N

F

A

L

Y

V

Y

R
x
E

E

E

V

V

P
x
Y

V

V

L

L

H

E

E

E

I

I

D

E

W

Y

L

M

I

I

K

Q

A

K

V

L

P

P

G

E

F

W

C

A

G

A

G

D

R

E

R

P

V

V

L

E

P

K

S

T

L

P

G

Q

Q

T

F

Q

K

Q

A

D

R

E

S

L

Y

Y

V

I

R

H

R

S

Q

E

P

P

L

L

G

G

R

V

V

V

V

L

A

V

Y

I

A

G

H

T

W

W

A
x
N

D
x
Y

P

P

F

F

R
x
N

S
x
L

L

T

L

I

V

Q

P

P

L

M

A

V

D

G

A

A

I

I

G

A

A

A

G

G

N

N

A

A

V

V

L

L

R
x
K

P

P

S

S

A

E

E

L

A

S

P

E

A

N

T

M

A

A

E

S

M

L

V

L

T

A

R

T

M

I

V

I

R

P

Q

Q

Y

Y

F

L

E

D

P

K

E

D

H

L

V

Y

A

P

V

V

V

I

G

N

G

G

A

V

E

P

T

E

D

T

E

T

A

E

L

L

A

K

T

E

A

R

P

F

D

D

F

H

V

I

W

L

Y

Y

D

T

G

G

D

S

A

T

R

G

G

V

A

G

R

K

T

I

I

I

A

M

V

T

L

A

A

A

P

K

T

H

L

L

T

T

P

P

Y

V

A

T

A

L

I
x
E

T

L

G

G

P

K

S

S

A

P

A

C

L

Y

V

V

H

D

G

K

D

N

A

C

D

D

M

L

A

D

M

V

A

A

A

C

R

R

I

I

V

A

W

W

A

G

K

K

F

F

L

M

H

N

A

S

G

G

Q

Q

L
x
T

R
x
C

A

V

A

A

P

P

D

D

V

Y

L

I

L

L

V

C

Q

D

R

P

T

S

V

I

L

Q

D

N

R

Q

V

I

L

V

D

E

A

K

L

L

R

K

T

K

E

S

L

L

E

K

R

E

A

F

F

Y

G

G

P

E

Q

D

P

A

R

K

T

K

S

S

A

R

D

D

F

Y

G

G

R

R

M

I

V

I

S

S

A

A

A

R

G

H

F

F

A

Q

R

R

-

V

-

M

-

G

-

L

-

I

Q

E

A

G

E

Q

R

K

L

V

A

A

I

Y

G

G

R

-

A

-

L

-

P

-

F

-

G

G

P

T

G

G

D

D

A

A

N

T

Q

R

P

-

D

-

R

-

A

-

S

-

L

-

Y

Y

V

I

P

A

P

P

T

T

L

I

L

L

T

T

D

D

V

V

P

D

D

P

D

Q

S

S

P

P

V

V

L

M

R

Q

E

E

E
|
E

G

I

F

F

G

G

P

P

V

V

L

L

V

P

V

I

R

V

P

C

Y

V

T

R

R

S

L

L

D

E

E

E

A

A

T

I

A

Q

F

F

L

I

A

N

G

Q

L

R

P

E

A

K

L

P

T

L

A

A

L

L

Y

Y

A

M

F

F

T

S

A

N

H

D

A

K

R

V

G

I

E

K

R

K

L

M

M

I

E

A

N

E

T

T

R

S

A

S

G

G

A

G

V

V

L

A

I

A

N

N

D

D

A

V

A
x
I

T

V

H

H

L
x
I

A

T

N

L

P

H

R

S

L

L

P

P

Q
x
F

G

G

V

V

G

G

E

N

T

S

G

G

H

M

G

G

A

S

M
x
Y

A
x
H

G

G

P

K

A

K

G

S

L

F

A

E

T

T

F

F

S

S

A

H

P

R

R

R

A

S

T

C

A

L

V

V

active site: N114 (≠ A112), K137 (≠ R135), E209 (≠ I207), C243 (≠ R241), E333 (= E337), Y412 (≠ M416)
binding (3S)-1-{[4-(1,3-benzodioxol-5-ylmethyl)piperazin-1-yl]methyl}-3-hydroxy-1,3-dihydro-2H-indol-2-one: E61 (≠ R59), Y65 (≠ P63), Y115 (≠ D113), N118 (≠ R116), L119 (≠ S117), E209 (≠ I207), T242 (≠ L240), C243 (≠ R241), I391 (≠ A395), I394 (≠ L398), F401 (≠ Q405), H413 (≠ A417)

P30838 Aldehyde dehydrogenase, dimeric NADP-preferring; ALDHIII; Aldehyde dehydrogenase 3; Aldehyde dehydrogenase family 3 member A1; EC 1.2.1.5 from Homo sapiens (Human) (see 4 papers)
33% identity, 93% coverage: 7:433/460 of query aligns to 10:430/453 of P30838

query
sites
P30838

R

R

Q

A

K

R

A

A

F

A

I

F

A

S

S

S

G

G

A

R

V

T

L

R

P

P

S

L

G

Q

A

F

R

R

V

I

D

Q

A

Q

L

L

R

E

R

A

L

L

R

Q

E

R

G

L

V

I

Q

Q

G

E

Y

Q

R

E

D

Q

R

E

L

L

A

V

D

G

A

A

I

L

R

A

A

A

D

D

Y

L

G

H

R

K

P

N

E

E

H

W

P

N

F

A

L

Y

V

Y

R

E

E

E

V

V

P

Y

V

V

L

L

H

E

E

E

I

I

D

E

W

Y

L

M

I

I

K

Q

A

K

V

L

P

P

G

E

F

W

C

A

G

A

G

D

R

E

R

P

V

V

L

E

P

K

S

T

L

P

G

Q

Q

T

F

Q

K

Q

A

D

R

E

S

L

Y

Y

V

I

R

H

R

S

Q

E

P

P

L

L

G

G

R

V

V

V

V

L

A

V

Y

I

A

G

H

T

W

W

A

N

D

Y

P

P

F

F

R

N

S

L

L

T

L

I

V

Q

P

P

L

M

A

V

D

G

A

A

I

I

G

A

A

A

G

G

N

N

A
x
S

V

V

L

L

R

K

P

P

S

S

A

E

E

L

A

S

P

E

A

N

T

M

A

A

E

S

M

L

V

L

T

A

R

T

M

I

V

I

R

P

Q

Q

Y

Y

F

L

E

D

P

K

E

D

H

L

V

Y

A

P

V

V

V

I

G

N

G

G

A

V

E

P

T

E

D

T

E

T

A

E

L

L

A

K

T

E

A

R

P

F

D

D

F

H

V

I

W

L

Y

Y

D

T

G

G

D

S

A

T

R

G

G

V

A

G

R

K

T

I

I

I

A

M

V

T

L

A

A

A

P

K

T

H

L

L

T

T

P

P

Y

V

A

T

A

L

I
x
E

T

L

G

G

P

K

S

S

A

P

A

C

L

Y

V

V

H

D

G

K

D

N

A

C

D

D

M

L

A

D

M

V

A

A

A

C

R

R

I

I

V

A

W

W

A

G

K

K

F

F

L

M

H

N

A

S

G

G

Q

Q

L

T

R
x
C

A

V

A

A

P

P

D

D

V

Y

L

I

L

L

V

C

Q

D

R

P

T

S

V

I

L

Q

D

N

R

Q

V

I

L

V

D

E

A

K

L

L

R

K

T

K

E

S

L

L

E

K

R

E

A

F

F

Y

G

G

P

E

Q

D

P

A

R

K

T

K

S

S

A

R

D

D

F

Y

G

G

R

R

M

I

V

I

S

S

A

A

A

R

G

H

F

F

A

Q

R

R

-

V

-

M

-

G

-

L

-

I

Q

E

A

G

E

Q

R

K

L

V

A

A

I

Y

G

G

R

-

A

-

L

-

P

-

F

-

G

G

P

T

G

G

D

D

A

A

N

T

Q

R

P

-

D

-

R

-

A

-

S

-

L

-

Y

Y

V

I

P

A

P

P

T

T

L

I

L

L

T

T

D

D

V

V

P

D

D

P

D

Q

S

S

P
|
P

V

V

L

M

R

Q

E

E

G

I

F

F

G

G

P

P

V

V

L

L

V

P

V

I

R

V

P

C

Y

V

T

R

R

S

L

L

D

E

E

E

A

A

T

I

A

Q

F

F

L

I

A

N

G

Q

L

R

P

E

A

K

L

P

T

L

A

A

L

L

Y

Y

A

M

F

F

T

S

A

N

H

D

A

K

R

V

G

I

E

K

R

K

L

M

M

I

E

A

N

E

T

T

R

S

A

S

G

G

A

G

V

V

L

A

I

A

N

N

D

D

A

V

A

I

T

V

H

H

L

I

A

T

N

L

P

H

R

S

L

L

P

P

Q

F

G

G

V

V

G

G

E

N

T

S

G

G

H

M

G

G

A

S

M

Y

A

H

G

G

P

K

A

K

G

S

L

F

A

E

T

T

F

F

S

S

A

H

P

R

R

R

A

S

T

C

A

L

V

V

S134 (≠ A131) to A: in dbSNP:rs887241
E210 (≠ I207) active site
C244 (≠ R241) active site; mutation to S: Abolishes activity.
P329 (= P332) to A: in allele ALDH3A1*2; dbSNP:rs2228100

E9Q3E1 Aldehyde dehydrogenase family 3 member B2; Aldehyde dehydrogenase 8; EC 1.2.1.3 from Mus musculus (Mouse) (see paper)
32% identity, 94% coverage: 2:435/460 of query aligns to 19:445/479 of E9Q3E1

query
sites
E9Q3E1

A

A

A

T

L

L

A

H

A

S

R

L

Q

R

K

E

A

A

F

F

I

N

A

A

S

-

G

G

A

R

V

T

L

R

P

P

S

T

G

E

A

F

R

R

V

T

D

A

A

Q

L

L

R

R

R

S

L

L

R

G

E

R

G

F

V

L

Q

Q

G

E

Y

N

R

K

D

E

R

L

L

L

A

Q

D

D

A

A

I

L

R

A

A

K

D

D

Y

V

G

G

R

K

P

S

E

G

H

F

P

E

F

S

L

D

V

M

R

S

E

E

V

I

P

L

V

C

L

E

H

N

E

E

I

V

D

D

W

L

L

A

I

L

K

K

A

N

V

L

P

Q

G

T

F

W

C

M

G

K

G

D

R

E

R

P

V

V

L

S

P

T

S

N

L

L

G

L

Q

T

F

K

K

L

A

S

R

S

S

A

Y

F

V

I

R

R

R

K

Q

E

P

P

L

F

G

G

R

L

V

V

V

L

A

I

Y

I

A

A

H

P

W

W

A

N

D

Y

P

P

F

V

R

N

S

L

L

M

L

I

V

I

P

P

L

L

A

V

D

G

A

A

I

I

G

A

A

A

G

G

N

N

A

C

V

V

L

L

R

K

P

P

S

S

A

E

E

I

A

S

P

K

A

N

T

T

A

E

E

K

M

V

V

L

T

A

R

E

M

L

V

L

R

P

Q

Q

Y

Y

F

L

E

D

P

Q

E

S

H

C

V

F

A

A

V

V

V

M

G

L

G

G

A

P

E

E

T

E

D

T

E

R

A

Q

L

L

A

E

T

H

A

K

P

F

D

D

F

Y

V

I

W

F

Y

F

D

T

G

G

D

S

A

P

R

R

G

V

A

G

R

K

T

I

I

V

A

M

V

T

L

A

A

A

P

K

T

H

L

L

T

T

P

P

Y

I

A

T

A

L

I

E

T

L

G

G

P

K

S

N

A

P

A

C

L

Y

V

V

H

D

G

D

D

N

A

C

D

D

M

P

A

Q

M

T

A

V

A

A

R

N

R

R

I

V

V

A

W

W

A

F

K

R

F

Y

L

F

H

N

A

A

G

G

Q

Q

L

T

R

C

A

V

A

A

P

P

D

D

V

Y

L

I

L

L

V

C

Q

S

R

Q

T

E

V

M

L

Q

D

E

R

R

V

L

L

V

D

P

A

A

L

L

R

Q

T

N

E

S

L

I

E

T

R

R

A

F

F

Y

G

G

P

D

Q

N

P

P

R

Q

T

T

S

S

A

P

D

N

F

L

G

G

R

R

M

I

V

I

S

N

A

Q

A

K

G

H

F

F

A

K

R

R

Q

L

A

Q

E

G

R

L

L

L

A

G

I

C

G

G

R

R

A

V

L

A

P

I

F

G

G

G

P

-

G

-

D

-

A

-

N

-

Q

Q

P

S

D

D

R

E

A

G

S

E

L

R

Y

Y

V

I

P

A

P

P

T

T

L

V

L

L

T

V

D

D

V

V

P

Q

D

E

D

T

S

E

P

P

V

V

L

M

R

Q

E

E

G

I

F

F

G

G

P

P

V

I

L

L

V

P

V

L

R

V

P

T

Y

V

T

R

R

S

L

L

D

D

E

E

A

A

T

I

A

E

F

F

L

I

A

N

G

R

L

R

P

E

A

K

L

P

T

L

A

A

L

L

Y

Y

A

A

F

F

T

S

T

N

A

N

H

N

A

Q

R

V

G

V

E

N

R

Q

L

M

M

L

E

E

N

R

T

T

R

S

A

S

G

G

A

G

V

F

L

G

I

G

N

N

D

D

A

G

A

F

T

L

H

Y

L

L

A

T

N

L

P

P

R

A

L

L

P

P

Q

L

G

G

V

V

G

G

E

N

T

S

G

G

H

M

G

G

A

R

M

Y

A

H

G

G

P

K

A

F

G

S

L

F

A

D

T

T

F

F

S

S

A

H

P

H

R

R

A

A

T

C

A

L

V

L

G

R

S

S

Sites not aligning to the query:

462 W→A: Reduces lipid droplet localization.
469 W→A: Reduces lipid droplet localization.
476 C→S: Reduces lipid droplet localization.

1ad3A Class 3 aldehyde dehydrogenase complex with nicotinamide- adenine-dinucleotide (see paper)
31% identity, 93% coverage: 7:435/460 of query aligns to 8:430/446 of 1ad3A

query
sites
1ad3A

R

R

Q

A

K

R

A

E

F

A

I

F

A

N

S

S

G

G

A

K

V

T

L

R

P

S

S

L

G

Q

A

F

R

R

V

I

D

Q

A

Q

L

L

R

E

R

A

L

L

R

Q

E

R

G

M

V

I

Q

N

G

E

Y

N

R

L

D

K

R

S

L

I

A

S

D

G

A

A

I

L

R

A

A

S

D

D

Y

L

G

G

R

K

P

N

E

E

H

W

P

T

F

S

L

Y

V

Y

R

E

E

E

V

V

V

A

P

H

V

V

L

L

H

E

E

E

I

L

D

D

W

T

L

T

I

I

K

K

A

E

V

L

P

P

G

D

F

W

C

A

G

E

G

D

R

E

R

P

V

V

L

A

P

K

S

T

L

R

G

Q

Q

T

F

Q

K

Q

A

D

R

D

S

L

Y

Y

V

I

R

H

R

S

Q

E

P

P

L

L

G

G

R

V

V

V

V

L

A

V

Y

I

A

G

H
x
A

W
|
W

A
x
N

D

Y

P

P

F

F

R

N

S

L

L

T

L

I

V

Q

P

P

L

M

A

V

D

G

A

A

I

V

G

A

A

A

G

G

N

N

A

A

V

V

V

I

L

L

R
x
K

P

P

S

S

A
x
E

E
x
V

A

S

P

G

A

H

T

M

A

A

E

D

M

L

V

L

T

A

R

T

M

L

V

I

R

P

Q

Q

Y

Y

F

M

E

D

P

Q

E

N

H

L

V

Y

A

L

V

V

G

K

G

G

G
|
G

A
x
V

E

P

T

E

D
x
T

E

T

A

E

L

L

A

K

T

E

A

R

P

F

D

D

F

H

V

I

W

M

Y

Y

D

T

G
|
G

D
x
S

A

T

R

A

G
x
V

A

G

R

K

T
x
I

I

V

A

M

V

A

L

A

A

A

P

K

T

H

L

L

T

T

P

P

Y

V

A

T

A

L

I
x
E

T

L

G

G

P

K

S

S

A

P

A

C

L

Y

V

V

H

D

G

K

D

D

A

C

D

D

M

L

A

D

M

V

A

A

A

C

R

R

I

I

V

A

W

W

A

G

K

K

F

F

L

M

H

N

A

S

G

G

Q

Q

L

T

R
x
C

A

V

A

A

P

P

D

D

V

Y

L

I

L

L

V

C

Q

D

R

P

T

S

V

I

L

Q

D

N

R

Q

V

I

L

V

D

E

A

K

L

L

R

K

T

K

E

S

L

L

E

K

R

D

A

F

F

Y

G

G

P

E

Q

D

P

A

R

K

T

Q

S

S

A

R

D

D

F

Y

G

G

R

R

M

I

V

I

S

N

A

D

A
x
R

G
x
H

F

F

A

Q

R
|
R

Q

-

A

V

E

K

R

G

L

L

A

I

I

D

G

N

R

Q

A

K

L

V

P

A

F

H

G

G

P

-

G

-

D

-

A

-

N

G

Q

T

P

W

D

D

R

Q

A

S

S

S

L

R

Y

Y

V

I

P

A

P

P

T

T

L

I

L

L

T

V

D

D

V

V

P

D

D

P

D

Q

S

S

P

P

V

V

L

M

R

Q

E

E

E
|
E

G

I

F
|
F

G

G

P

P

V

V

L

M

V

P

V

I

R

V

P

C

Y

V

T

R

R

S

L

L

D

E

E

E

A

A

T

I

A

Q

F

F

L

I

A

N

G

Q

L

R

P

E

A

K

L

P

T

L

A

A

L

L

Y

Y

A

V

F

F

T

S

T

N

A

N

H

E

A

K

R

V

G

I

E

K

R

K

L

M

M

I

E

A

N

E

T

T

R

S

A

S

G

G

A

G

V

V

L

T

I

A

N

N

D

D

A

V

A

I

T

V

H

H

L

I

A

T

N

V

P

P

R

T

L

L

P

P

Q

F

G

G

V

V

G

G

E

N

T

S

G

G

H

M

G

G

A

A

M
x
Y

A

H

G

G

P

K

A

K

G

S

L

F

A

E

T

T

F

F

S

S

A

H

P

R

R

R

A

S

T

C

A

L

V

V

G

K

S

S

active site: N113 (≠ A112), K136 (≠ R135), E208 (≠ I207), C242 (≠ R241), E332 (= E337), Y411 (≠ M416)
binding nicotinamide-adenine-dinucleotide: A111 (≠ H110), W112 (= W111), N113 (≠ A112), E139 (≠ A138), V140 (≠ E139), G167 (= G166), V168 (≠ A167), T171 (≠ D170), G186 (= G185), S187 (≠ D186), V190 (≠ G189), I193 (≠ T192), R287 (≠ A286), H288 (≠ G287), R291 (= R290), E332 (= E337), F334 (= F339)

J3QMK6 Aldehyde dehydrogenase family 3 member B3; EC 1.2.1.3 from Mus musculus (Mouse) (see paper)
30% identity, 93% coverage: 7:435/460 of query aligns to 23:445/479 of J3QMK6

query
sites
J3QMK6

R

R

Q

L

K

K

A

E

F

A

I

F

A

N

S

T

G

G

A

K

V

T

L

K

P

T

S

A

G

K

A

F

R

R

V

A

D

E

A

Q

L

L

R

Q

R

S

L

L

R

G

E

R

G

F

V

L

Q

Q

G

D

Y

N

R

S

D

K

R

Q

L

L

A

H

D

D

A

A

I

L

R

D

A

G

D

D

Y

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Sites not aligning to the query:

462:463 RR→AA: Reduces membrane localization.

Q80VQ0 Aldehyde dehydrogenase family 3 member B1; Aldehyde dehydrogenase 7; EC 1.2.1.28; EC 1.2.1.5; EC 1.2.1.7 from Mus musculus (Mouse) (see paper)
30% identity, 94% coverage: 23:455/460 of query aligns to 26:454/468 of Q80VQ0

query
sites
Q80VQ0

R

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