SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 8501944 FitnessBrowser__Miya:8501944 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
60% identity, 100% coverage: 1:310/310 of query aligns to 1:310/310 of P9WP55

query
sites
P9WP55

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K44 (= K44) modified: N6-(pyridoxal phosphate)lysine
N74 (= N74) binding
GTGGT 178:182 (= GTGGT 178:182) binding
S266 (= S266) binding

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
61% identity, 98% coverage: 1:305/310 of query aligns to 1:305/306 of 2q3dA

query
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2q3dA

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active site: K44 (= K44), S266 (= S266), P293 (≠ C293)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K44), T71 (= T71), S72 (= S72), G73 (= G73), N74 (= N74), T75 (= T75), Q144 (= Q144), G176 (= G176), V177 (= V177), G178 (= G178), T179 (= T179), G180 (= G180), G181 (= G181), T182 (= T182), Q221 (= Q221), G222 (= G222), I223 (= I223), S266 (= S266), P293 (≠ C293), D294 (= D294)

3zeiA Structure of the mycobacterium tuberculosis o-acetylserine sulfhydrylase (oass) cysk1 in complex with a small molecule inhibitor (see paper)
60% identity, 97% coverage: 1:300/310 of query aligns to 1:300/300 of 3zeiA

query
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3zeiA

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active site: K44 (= K44), S266 (= S266), P293 (≠ C293)
binding 3-[(Z)-[(5Z)-5-[[2-(2-hydroxy-2-oxoethyloxy)phenyl]methylidene]-3-methyl-4-oxidanylidene-1,3-thiazolidin-2-ylidene]amino]benzoic acid: T71 (= T71), S72 (= S72), G73 (= G73), T75 (= T75), M122 (= M122), I126 (= I126), Q144 (= Q144), F145 (= F145), K215 (≠ P215), G222 (= G222), G224 (= G224), A225 (= A225), F227 (= F227)
binding pyridoxal-5'-phosphate: V43 (= V43), K44 (= K44), N74 (= N74), G176 (= G176), V177 (= V177), G178 (= G178), T179 (= T179), G180 (= G180), G181 (= G181), T182 (= T182), Q221 (= Q221), G222 (= G222), I223 (= I223), S266 (= S266), P293 (≠ C293), D294 (= D294)

2q3cA 2.1 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) holoenzyme from mycobacterium tuberculosis in complex with the inhibitory peptide dfsi (see paper)
60% identity, 97% coverage: 1:300/310 of query aligns to 1:300/300 of 2q3cA

query
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2q3cA

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x
P

D

D

T

F

G

G

E

E

R

R

Y

Y

L

L

active site: K44 (= K44), S266 (= S266), P293 (≠ C293)
binding : K44 (= K44), T71 (= T71), S72 (= S72), G73 (= G73), N74 (= N74), T75 (= T75), G121 (= G121), M122 (= M122), Q144 (= Q144), F145 (= F145), G178 (= G178), K215 (≠ P215), Q221 (= Q221), G222 (= G222), G224 (= G224), A225 (= A225)

4aecA Crystal structure of the arabidopsis thaliana o-acetyl-serine-(thiol)- lyasE C (see paper)
57% identity, 99% coverage: 1:308/310 of query aligns to 11:319/323 of 4aecA

query
sites
4aecA

M

L

E

N

I

I

A

A

R

D

D

N

M

V

T

S

E

Q

L

L

V

I

G

G

R

K

T

T

P

P

L

M

V

V

R

Y

L

L

N

N

R

S

V

I

S

A

E

K

G

G

C

C

A

V

A

A

E

N

V

I

V

A

A

A

K

K

L

L

E

E

F

I

N

M

N

E

P

P

C

C

A

C

S

S

V
|
V

K
|
K

D

D

R

R

I

I

A

G

L

Y

A

S

M

M

I

V

D

T

G

D

A

A

M

E

E

Q

R

K

G

G

E

F

L

I

A

S

P

P

G

G

-

K

G

S

L

V

L

L

V

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

V

I

G

G

L

L

A

A

F

F

V

I

A

A

V

S

R

R

G

G

L

Y

T

R

L

L

V

I

L

L

T

T

M

M

P

P

E

A

S

S

M

M

S

S

N

M

E

E

R

R

K

R

A

V

L

L

R

K

G

A

F

F

G

G

A

A

R

E

L

L

V

V

L

L

T

T

P

D

A

P

S

A

K

K

G

G

M

M

R

T

G

G

A

A

I

V

E

Q

E

K

A

A

E

E

R

E

I

I

V

L

A

K

E

N

T

T

P

P

G

D

A

A

V

Y

M

M

L

L

G

Q

Q

Q

F

F

V

D

N

N

P

P

D

A

N

N

P

P

L

K

V

I

H

H

R

Y

K

E

T

T

A

G

E

P

E

E

I

I

W

W

A

D

D

D

T

T

D

K

G

G

K

K

V

V

D

D

A

I

F

F

V

V

A

A

G
|
G

V
x
I

G
|
G

T
|
T

G
|
G

T
|
T

V

I

T

T

G

G

T

V

G

G

R

R

R

F

L

I

R

K

E

E

L

K

N

N

P

P

D

K

I

T

K

Q

V

V

F

I

A

G

V

V

E

E

P

P

D

T

E

E

S

S

P

D

V

I

L

L

S

S

G

G

A

K

P

P

G

G

P

P

H

H

P

K

I

I

Q
|
Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

P

K

V

N

L

L

D

D

T

Q

K

K

V

I

Y

M

D

D

E

E

I

V

I

I

R

A

V

I

P

S

G

S

K

E

D

E

A

A

L

I

V

E

T

T

A

A

R

K

R

Q

L

L

L

A

R

L

E

K

E

E

G

G

I

L

L

M

C

V

G

G

V

I

S
|
S

S

S

G

G

A

A

N

A

A

A

F

A

A

A

M

I

A

K

V

V

A

A

R

K

R

R

P

P

E

E

M

N

A

A

G

G

K

K

R

L

V

I

V

A

F

V

V

V

V

F

C
x
P

D
x
S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

P

L

L

F

F

T

Q

E

S

M

I

active site: K54 (= K44), S277 (= S266)
binding pyridoxal-5'-phosphate: V53 (= V43), K54 (= K44), N85 (= N74), G187 (= G176), I188 (≠ V177), G189 (= G178), T190 (= T179), G191 (= G180), G192 (= G181), T193 (= T182), Q232 (= Q221), G233 (= G222), S277 (= S266), P304 (≠ C293), S305 (≠ D294)

4lmaA Crystal structure analysis of o-acetylserine sulfhydrylase cysk1 from microcystis aeruginosa 7806 (see paper)
57% identity, 100% coverage: 1:310/310 of query aligns to 1:313/318 of 4lmaA

query
sites
4lmaA

M

M

E

R

I

I

A

A

R

H

D

D

M

V

T

T

E

E

L

L

V

V

G

G

R

R

T

T

P

P

L

L

V

V

R

Q

L

L

N

N

R

R

V

I

-

P

-

Q

S

A

E

E

G

G

C

C

A

L

A

G

E

R

V

I

V

V

A

M

K

K

L

L

E

E

F

G

N

M

N

N

P

P

C

A

A

A

S

S

V
|
V

K
|
K

D

D

R

R

I

I

A

G

L

T

A

H

M

M

I

I

D

N

G

S

A

A

M

E

E

K

R

A

G

G

E

L

L

I

A

N

P

P

-

E

G

T

L

V

L

L

V

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

V

I

G

A

L

L

A

A

F

M

V

T

A

A

V

A

R

K

G

G

L

Y

T

R

L

L

V

I

L

L

T

T

M

M

P

P

E

E

S

T

M

M

S

S

N

L

E

E

R

R

K

R

A

A

L

M

L

L

R

K

G

A

F

Y

G

G

A

A

R

T

L

L

V

E

L

L

T

T

P

P

A

G

S

S

K

Q

G

G

M

M

R

K

G

G

A

A

I

I

E

L

E

R

A

A

E

Q

R

Q

I

I

V

V

A

D

E

S

T

I

P

P

G

G

A

A

V

Y

M

M

L

L

G

Q

Q

Q

F

F

V

R

N

N

P

P

D

S

N

N

P

P

L

E

V

I

H

H

R

R

K

L

T

T

A

A

E

E

I

I

W

W

A

Q

D

D

T

T

D

E

G

G

K

Q

V

V

D

D

A

F

F

I

V

V

A

A

G
|
G

V
|
V

G
|
G

T
|
T

G
|
G

T
|
T

V

I

T

T

G

G

T

V

G

A

R

E

R

V

L

I

R

K

E

S

L

R

N

K

P

P

D

S

I

F

K

Q

V

V

F

V

A

A

V

V

E

E

P

P

D

F

E

N

S

S

P

P

V

V

L

I

S

S

G

G

A

N

P

P

G

G

P

P

H

H

P

K

I

I

Q
|
Q

G
|
G

I
|
I

G

G

A

A

G

G

F

F

V

I

P

P

P

E

V

V

L

L

D

R

T

T

K

D

V

L

Y

I

D

D

E

E

I

V

I

I

R

T

V

V

P

S

G

D

K

E

D

E

A

A

L

F

V

Q

T

F

A

G

R

R

L

L

L

A

R

K

E

E

G

G

I

L

L

L

C

S

G

G

V

I

S
|
S

S

S

G

G

A

A

N

N

A

L

F

C

A

A

M

I

A

Q

V

L

A

A

R

Q

R

R

P

P

E

E

M

N

A

E

G

G

K

K

R

L

V

I

V

V

F

V

V

I

V

Q

C
x
P

D
x
S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

L

L

M

F

F

T

Q

E

N

M

I

P

E

D

E

active site: K46 (= K44), S269 (= S266)
binding pyridoxal-5'-phosphate: V45 (= V43), K46 (= K44), N77 (= N74), G179 (= G176), V180 (= V177), G181 (= G178), T182 (= T179), G183 (= G180), G184 (= G181), T185 (= T182), Q224 (= Q221), G225 (= G222), I226 (= I223), S269 (= S266), P296 (≠ C293), S297 (≠ D294)

7n2tA O-acetylserine sulfhydrylase from citrullus vulgaris in the internal aldimine state, with citrate bound (see paper)
58% identity, 98% coverage: 3:305/310 of query aligns to 3:306/309 of 7n2tA

query
sites
7n2tA

I

I

A

A

R

K

D

D

M

V

T

T

E

E

L

L

V

I

G

G

R

N

T

T

P

P

L

L

V

V

R

Y

L

L

N

N

R

R

V

V

S

V

E

D

G

G

C

C

A

V

A

A

E

R

V

V

V

A

A

A

K

K

L

L

E

E

F

M

N

M

N

E

P

P

C

C

A

S

S

S

V

V

K

K

D

D

R

R

I

I

A

G

L

Y

A

S

M

M

I

I

D

S

G

D

A

A

M

E

E

N

R

K

G

G

E

L

L

I

A

T

P

P

G

G

-

E

G

S

L

V

L

L

V

I

E

E

P

P

T

T

S

S

G

G

N

N

T

T

G

G

V

I

G

G

L

L

A

A

F

F

V

I

A

A

V

A

R

K

G

G

L

Y

T

R

L

L

V

I

L

I

T

C

M

M

P

P

E

A

S

S

M

M

S

S

N

L

E

E

R

R

K

R

A

T

L

I

L

L

R

R

G

A

F

F

G

G

A

A

R

E

L

L

V

V

L

L

T

T

P

D

A

P

S

A

K

R

G

G

M

M

R

K

G

G

A

A

I

V

E

Q

E

K

A

A

E

E

R

E

I

I

V

K

A

A

E

K

T

T

P

P

G

N

A

S

V

Y

M

I

L

L

G

Q

Q

Q

F

F

V

E

N

N

P

P

D

A

N

N

P

P

L

K

V

I

H

H

R

Y

K

E

T

T

A

G

E

P

E

E

I

I

W

W

A

R

D

G

T

S

D

G

G

G

K

K

V

I

D

D

A

A

F

L

V

V

A

S

G

G

V

I

G

G

T

T

G

G

T

T

V

V

T

T

G

G

T

A

G

G

R

K

R

Y

L

L

R

K

E

E

L

Q

N

N

P

P

D

N

I

I

K

K

V

L

F

Y

A

G

V

V

E

E

P

P

D

V

E

E

S

S

P

A

V

I

L

L

S

S

G

G

A

K

P

P

G

G

P

P

H

H

P

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

P

G

V

V

L

L

D

D

T

V

K

N

V

L

Y

L

D

D

E

E

I

V

I

I

R

Q

V

V

P

S

G

S

K

E

D

E

A

S

L

I

V

E

T

T

A

A

R

K

R

L

L

L

L

A

R

L

E

K

E

E

G

G

I

L

L

L

C

V

G

G

V

I

S

S

G

G

A

A

N

A

A

A

F

A

A

A

M

I

A
x
R

V

I

A

A

R

K

R
|
R

P

P

E

E

M

N

A

A

G

G

K

K

R

L

V

I

V

V

F

A

V

V

V

F

C

P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

V

L

L

F

F

binding phosphate ion: R277 (≠ A276), R281 (= R280)

P47999 Cysteine synthase, chloroplastic/chromoplastic; At.OAS.7-4; Beta-substituted Ala synthase 2;1; ARAth-Bsas2;1; CSase B; AtCS-B; CS-B; O-acetylserine (thiol)-lyase; O-acetylserine sulfhydrylase; OAS-TL B; cpACS1; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
57% identity, 99% coverage: 1:308/310 of query aligns to 73:381/392 of P47999

query
sites
P47999

M

L

E

N

I

I

A

A

R

D

D

N

M

A

T

A

E

Q

L

L

V

I

G

G

R

K

T

T

P

P

L

M

V

V

R

Y

L

L

N

N

R

N

V

V

S

V

E

K

G

G

C

C

A

V

A

A

E

S

V

V

V

A

A

A

K

K

L

L

E

E

F

I

N

M

N

E

P

P

C

C

A

C

S

S

V

V

K

K

D

D

R

R

I

I

A

G

L

Y

A

S

M

M

I

I

D

T

G

D

A

A

M

E

E

E

R

K

G

G

E

L

L

I

A

T

P

P

G

G

-

K

G

S

L

V

L

L

V

V

E

E

P

S

T

T

S

S

G

G

N

N

T

T

G

G

V

I

G

G

L

L

A

A

F

F

V

I

A

A

V

S

R

K

G

G

L

Y

T

K

L

L

V

I

L

L

T

T

M

M

P

P

E

A

S

S

M

M

S

S

N

L

E

E

R

R

K

R

A

V

L

L

R

R

G

A

F

F

G

G

A

A

R

E

L

L

V

V

L

L

T

T

P

E

A

P

S

A

K

K

G

G

M

M

R

T

G

G

A

A

I

I

E

Q

E

K

A

A

E

E

R

E

I

I

V

L

A

K

E

K

T

T

P

P

G

N

A

S

V

Y

M

M

L

L

G

Q

Q

Q

F

F

V

D

N

N

P

P

D

A

N

N

P

P

L

K

V

I

H

H

R

Y

K

E

T

T

A

G

E

P

E

E

I

I

W

W

A

E

D

D

T

T

D

R

G

G

K

K

V

I

D

D

A

I

F

L

V

V

A

A

G

G

V

I

G

G

T

T

G

G

T

T

V

I

T

T

G

G

T

V

G

G

R

R

R

F

L

I

R

K

E

E

L

R

N

K

P

P

D

E

I

L

K

K

V

V

F

I

A

G

V

V

E

E

P

P

D

T

E

E

S

S

P

A

V

I

L

L

S

S

G

G

A

K

P

P

G

G

P

P

H

H

P

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

V

P

P

P

K

V

N

L

L

D

D

T

L

K

A

V

I

Y

V

D

D

E

E

I

Y

I

I

R

A

V

I

P

S

G

S

K

E

D

E

A

A

L

I

V

E

T

T

A

S

R

K

R

Q

L

L

L

A

R

L

E

Q

E

E

G

G

I

L

L

L

C

V

G

G

V

I

S

S

G

G

A

A

N

A

A

A

F

A

A

A

M

I

A

Q

V

V

A

A

R

K

R

R

P

P

E

E

M

N

A

A

G

G

K

K

R

L

V

I

V

A

F

V

V

V

V

F

C

P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

Q

L

L

F

F

T

Q

E

S

M

I

Sites not aligning to the query:

61 modified: N-acetylalanine

2isqA Crystal structure of o-acetylserine sulfhydrylase from arabidopsis thaliana in complex with c-terminal peptide from arabidopsis serine acetyltransferase (see paper)
57% identity, 98% coverage: 3:305/310 of query aligns to 3:306/320 of 2isqA

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active site: K44 (= K44), S267 (= S266)
binding pyridoxal-5'-phosphate: K44 (= K44), N75 (= N74), G177 (= G176), I178 (≠ V177), G179 (= G178), T180 (= T179), G181 (= G180), G182 (= G181), T183 (= T182), Q222 (= Q221), G223 (= G222), I224 (= I223), S267 (= S266), P294 (≠ C293), S295 (≠ D294)
binding : K44 (= K44), T72 (= T71), S73 (= S72), G74 (= G73), N75 (= N74), T76 (= T75), S98 (= S97), K121 (= K120), G122 (= G121), M123 (= M122), K124 (≠ R123), Q145 (= Q144), F146 (= F145), G179 (= G178), G217 (= G216), P218 (= P217), H219 (= H218), Q222 (= Q221), G223 (= G222), G225 (= G224), A226 (= A225), F228 (= F227)

P47998 Cysteine synthase 1; At.OAS.5-8; Beta-substituted Ala synthase 1;1; ARAth-Bsas1;1; CSase A; AtCS-A; Cys-3A; O-acetylserine (thiol)-lyase 1; OAS-TL A; O-acetylserine sulfhydrylase; Protein ONSET OF LEAF DEATH 3; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
57% identity, 98% coverage: 3:305/310 of query aligns to 5:308/322 of P47998

query
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P47998

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K46 (= K44) modified: N6-(pyridoxal phosphate)lysine; mutation to A: No cysteine synthase activity.
T74 (= T71) mutation to A: Strong reduction of cysteine synthase activity.; mutation to S: Reduction of cysteine synthase activity.
S75 (= S72) mutation S->A,N,T: Strong reduction of cysteine synthase activity.
N77 (= N74) binding ; mutation to A: Reduction of cysteine synthase activity.; mutation to D: Strong reduction of cysteine synthase activity.
T78 (= T75) mutation T->A,S: Reduction of cysteine synthase activity.
Q147 (= Q144) mutation Q->A,E: Strong reduction of cysteine synthase activity.
H157 (= H154) mutation H->Q,N: Slight reduction of cysteine synthase activity.
G162 (≠ A159) mutation to E: In old3-1; displays a early leaf death phenotype. Abolishes cysteine synthase activity.
GTGGT 181:185 (= GTGGT 178:182) binding
T182 (= T179) mutation T->A,S: Slight reduction of cysteine synthase activity.
T185 (= T182) mutation T->A,S: Strong reduction of cysteine synthase activity.
K217 (≠ A214) mutation to A: Impaired interaction with SAT1.
H221 (= H218) mutation to A: Impaired interaction with SAT1.
K222 (≠ P219) mutation to A: Impaired interaction with SAT1.
S269 (= S266) binding ; mutation to A: Strong reduction of cysteine synthase activity.; mutation to T: Reduction of cysteine synthase activity.

4lmbA Crystal structure analysis of o-acetylserine sulfhydrylase cysk2 complexed with cystine from microcystis aeruginosa 7806 (see paper)
57% identity, 99% coverage: 1:307/310 of query aligns to 1:310/310 of 4lmbA

query
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4lmbA

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active site: K46 (= K44), S269 (= S266)
binding cysteine: K46 (= K44), T74 (= T71), S75 (= S72), S75 (= S72), G76 (= G73), N77 (= N74), T78 (= T75), M101 (= M98), M125 (= M122), M125 (= M122), Q147 (= Q144), F148 (= F145), F148 (= F145), I223 (= I220), Q224 (= Q221), G225 (= G222), G225 (= G222), I226 (= I223), G227 (= G224), A228 (= A225)
binding pyridoxal-5'-phosphate: K46 (= K44), N77 (= N74), G179 (= G176), V180 (= V177), G181 (= G178), T182 (= T179), G183 (= G180), G184 (= G181), T185 (= T182), Q224 (= Q221), G225 (= G222), I226 (= I223), S269 (= S266), P296 (≠ C293), S297 (≠ D294)

1z7yA Crystal structure of the arabidopsis thaliana o-acetylserine sulfhydrylase k46a mutant (see paper)
56% identity, 98% coverage: 3:305/310 of query aligns to 3:306/320 of 1z7yA

query
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A

K

P

P

G

G

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P

H

H

P

K

I

I

Q
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Q

G
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G

I
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I

G

G

A

A

G

G

F

F

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I

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V

L

L

D

N

T

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K

D

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Y

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E

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D

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M

A

A

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R

Q

L

L

L

A

R

L

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K

E

E

G

G

I

L

L

L

C

V

G

G

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S

S

S

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G

A

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A

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A

A

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L

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x
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Y

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L

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F

active site: A44 (≠ K44), S267 (= S266)
binding n-[(3-hydroxy-2-methyl-5-{[(trihydroxyphosphoranyl)oxy]methyl}pyridin-4-yl)methylene]methionine: T72 (= T71), G74 (= G73), N75 (= N74), T76 (= T75), Q145 (= Q144), G177 (= G176), I178 (≠ V177), G179 (= G178), T180 (= T179), G181 (= G180), G182 (= G181), T183 (= T182), Q222 (= Q221), G223 (= G222), I224 (= I223), S267 (= S266), P294 (≠ C293), S295 (≠ D294)

Q93244 Cysteine synthase 1; O-acetylserine (thiol)-lyase 1; OAS-TL; EC 2.5.1.47 from Caenorhabditis elegans (see 2 papers)
53% identity, 99% coverage: 3:308/310 of query aligns to 7:313/341 of Q93244

query
sites
Q93244

I

I

A

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D

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M

A

T

A

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A

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L

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A

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D

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L

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x
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D

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I

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T

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G

T

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G

G

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R

R

Y

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L

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E

E

L

Q

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N

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P

D

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F

A

A

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V

E

E

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P

D

E

E

E

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A

V

I

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L

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S

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A

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A

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G

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G

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F

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L

D

D

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K

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Y

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R

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L

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E

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G

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L

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C

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S

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G

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A

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V

L

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M

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x
T

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V

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P75 (= P70) mutation to L: In n5537; severe loss of protein stability.
A88 (= A83) mutation to V: In n5522; severe loss of protein stability.
S144 (≠ A139) mutation to F: In mr26; susceptible to high levels of hydrogen sulfide.
G181 (= G176) mutation to E: In n5521 and mr23; severe loss of protein stability. Susceptible to high levels of hydrogen sulfide.
G183 (= G178) mutation to R: In n5515; severe loss of protein stability.
G229 (= G224) mutation to E: In mr33; susceptible to high levels of hydrogen sulfide.
R259 (= R254) mutation to K: In n5519; no loss of protein stability. No effect on enzyme activity.
S272 (= S267) mutation to F: In mr29; susceptible to high levels of hydrogen sulfide.
T295 (≠ F290) mutation to I: In mr39; susceptible to high levels of hydrogen sulfide.

8b9yC Cysteine synthase from trypanosoma cruzi with plp and oas (see paper)
53% identity, 99% coverage: 3:310/310 of query aligns to 10:317/330 of 8b9yC

query
sites
8b9yC

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A

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L

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D

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K

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G

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N

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L

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L

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E

E

A

A

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F

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A

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E

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A

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V

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L

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G

A

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I

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Q

G
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G

I

I

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A

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F

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P

P

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A

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A