SitesBLAST
Comparing 8502147 DvMF_2858 short-chain dehydrogenase/reductase SDR (RefSeq) to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8hfkA Crystal structure of cbar mutant (h162f) in complex with NADP+ and halogenated aryl ketone (see paper)
39% identity, 97% coverage: 8:245/245 of query aligns to 11:259/259 of 8hfkA
- binding 2-bromanyl-1-(4-bromanyl-2-oxidanyl-phenyl)ethanone: S143 (≠ T140), N144 (≠ S141), T145 (≠ V142), F153 (≠ Y150), Y156 (= Y153), G187 (= G184), M193 (≠ L190), V197 (vs. gap), A259 (= A245)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G12), R18 (= R15), I20 (= I17), A40 (= A37), N41 (≠ S38), S42 (≠ N39), D66 (= D63), N93 (≠ S90), S94 (≠ A91), L116 (≠ T113), T141 (≠ L138), Y156 (= Y153), K160 (= K157), P186 (= P183), G187 (= G184), G188 (≠ P185), T189 (≠ V186), T191 (= T188), M193 (≠ L190)
7yb2D Crystal structure of anthrol reductase (cbar) in complex with NADP+ and emodin (see paper)
39% identity, 97% coverage: 8:245/245 of query aligns to 15:264/264 of 7yb2D
- binding 3-methyl-1,6,8-trihydroxyanthraquinone: S147 (= S141), Y161 (= Y153), G193 (≠ P185), M198 (≠ L190), F199 (= F191), V202 (vs. gap), S203 (vs. gap), Y206 (vs. gap)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G19 (= G12), R22 (= R15), G23 (= G16), I24 (= I17), Y43 (= Y36), A44 (= A37), N45 (≠ S38), S46 (≠ N39), D70 (= D63), V71 (= V64), N97 (≠ S90), S98 (≠ A91), L120 (≠ T113), T145 (≠ S139), S147 (= S141), Y161 (= Y153), K165 (= K157), P191 (= P183), G192 (= G184), T194 (≠ V186), T196 (= T188), M198 (≠ L190)
8hfjC Crystal structure of cbar mutant (h162f) in complex with NADP+ and a bulky 1,3-cyclodiketone (see paper)
39% identity, 96% coverage: 8:243/245 of query aligns to 11:258/260 of 8hfjC
- binding 2-methyl-2-[(4-methylphenyl)methyl]cyclopentane-1,3-dione: N144 (≠ V142), T145 (≠ I143), F154 (≠ Y150), G189 (≠ P185), V198 (vs. gap)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G12), R18 (= R15), I20 (= I17), Y39 (= Y36), A40 (= A37), N41 (≠ S38), S42 (≠ N39), D66 (= D63), V67 (= V64), N93 (≠ S90), S94 (≠ A91), L116 (≠ T113), T141 (≠ S139), Y157 (= Y153), K161 (= K157), P187 (= P183), T190 (≠ V186), T192 (= T188), M194 (≠ L190)
Sites not aligning to the query:
3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
39% identity, 98% coverage: 6:244/245 of query aligns to 5:244/246 of 3osuA
3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
39% identity, 98% coverage: 6:244/245 of query aligns to 2:237/239 of 3sj7A
- active site: G12 (= G16), S138 (≠ T140), Q148 (≠ Y150), Y151 (= Y153), K155 (= K157)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), S10 (= S14), R11 (= R15), I13 (= I17), N31 (= N35), Y32 (= Y36), A33 (= A37), G34 (≠ S38), S35 (≠ N39), A58 (= A62), N59 (≠ D63), V60 (= V64), N86 (≠ S90), A87 (= A91), T109 (= T113), S138 (≠ T140), Y151 (= Y153), K155 (= K157), P181 (= P183), G182 (= G184)
4fj1B Crystal structure of the ternary complex between a fungal 17beta- hydroxysteroid dehydrogenase (holo form) and genistein (see paper)
37% identity, 97% coverage: 8:245/245 of query aligns to 10:259/259 of 4fj1B
- active site: G18 (= G16), S142 (= S141), N143 (≠ V142), H153 (≠ Y150), Y156 (= Y153), K160 (= K157), Y201 (vs. gap)
- binding genistein: G188 (≠ P185), F194 (= F191), S198 (vs. gap), Y201 (vs. gap), I202 (vs. gap), M216 (≠ A202), A217 (≠ I203)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G14 (= G12), R17 (= R15), G18 (= G16), I19 (= I17), A39 (= A37), N40 (≠ S38), S41 (≠ N39), I66 (≠ V64), N92 (≠ S90), S93 (≠ A91), G94 (= G92), L115 (≠ T113), T140 (≠ S139), S142 (= S141), Y156 (= Y153), K160 (= K157), G187 (= G184), T189 (≠ V186), T191 (= T188), M193 (≠ L190)
4fj2B Crystal structure of the ternary complex between a fungal 17beta- hydroxysteroid dehydrogenase (holo form) and biochanin a (see paper)
37% identity, 97% coverage: 8:245/245 of query aligns to 11:260/260 of 4fj2B
- active site: G19 (= G16), S143 (= S141), N144 (≠ V142), H154 (≠ Y150), Y157 (= Y153), K161 (= K157), Y202 (vs. gap)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G12), R18 (= R15), G19 (= G16), I20 (= I17), A40 (= A37), N41 (≠ S38), S42 (≠ N39), I67 (≠ V64), N93 (≠ S90), S94 (≠ A91), G95 (= G92), L116 (≠ T113), T141 (≠ S139), Y157 (= Y153), K161 (= K157), G188 (= G184), G189 (≠ P185), T190 (≠ V186), T192 (= T188), M194 (≠ L190)
- binding 5,7-dihydroxy-3-(4-methoxyphenyl)-4H-chromen-4-one: G189 (≠ P185), F195 (= F191), V198 (vs. gap), S199 (vs. gap), Y202 (vs. gap), I203 (vs. gap), M217 (≠ A202), A218 (≠ I203)
4fj0D Crystal structure of the ternary complex between a fungal 17beta- hydroxysteroid dehydrogenase (holo form) and 3,7-dihydroxy flavone (see paper)
37% identity, 97% coverage: 8:245/245 of query aligns to 12:261/261 of 4fj0D
- active site: G20 (= G16), S144 (= S141), N145 (≠ V142), H155 (≠ Y150), Y158 (= Y153), K162 (= K157), Y203 (vs. gap)
- binding 3,7-dihydroxy-2-phenyl-4H-chromen-4-one: S144 (= S141), N145 (≠ V142), G190 (≠ P185), F196 (= F191), S200 (vs. gap), Y203 (vs. gap), A219 (≠ I203)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G16 (= G12), R19 (= R15), G20 (= G16), I21 (= I17), A41 (= A37), N42 (≠ S38), S43 (≠ N39), I68 (≠ V64), N94 (≠ S90), S95 (≠ A91), G96 (= G92), L117 (≠ T113), T142 (≠ S139), Y158 (= Y153), K162 (= K157), P188 (= P183), G189 (= G184), G190 (≠ P185), T191 (≠ V186), T193 (= T188), M195 (≠ L190)
3qwiA Crystal structure of a 17beta-hydroxysteroid dehydrogenase (holo form) from fungus cochliobolus lunatus in complex with NADPH and coumestrol (see paper)
37% identity, 97% coverage: 8:245/245 of query aligns to 11:260/260 of 3qwiA
- active site: G19 (= G16), S143 (= S141), N144 (≠ V142), H154 (≠ Y150), Y157 (= Y153), K161 (= K157), Y202 (vs. gap)
- binding Coumestrol: F149 (= F147), G189 (≠ P185), M194 (≠ L190), Y202 (vs. gap), I203 (vs. gap), A218 (≠ I203)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G12), R18 (= R15), I20 (= I17), A40 (= A37), N41 (≠ S38), S42 (≠ N39), I67 (≠ V64), N93 (≠ S90), S94 (≠ A91), G95 (= G92), L116 (≠ T113), T141 (≠ S139), Y157 (= Y153), K161 (= K157), P187 (= P183), G188 (= G184), G189 (≠ P185), T190 (≠ V186), T192 (= T188), M194 (≠ L190)
3qwhA Crystal structure of the 17beta-hydroxysteroid dehydrogenase from cochliobolus lunatus in complex with NADPH and kaempferol (see paper)
37% identity, 97% coverage: 8:245/245 of query aligns to 11:260/260 of 3qwhA
- active site: G19 (= G16), S143 (= S141), N144 (≠ V142), H154 (≠ Y150), Y157 (= Y153), K161 (= K157), Y202 (vs. gap)
- binding 3,5,7-trihydroxy-2-(4-hydroxyphenyl)-4h-chromen-4-one: N144 (≠ V142), F149 (= F147), G189 (≠ P185), F195 (= F191), S199 (vs. gap), Y202 (vs. gap), I203 (vs. gap), A218 (≠ I203)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G12), R18 (= R15), G19 (= G16), I20 (= I17), A40 (= A37), N41 (≠ S38), S42 (≠ N39), D66 (= D63), I67 (≠ V64), N93 (≠ S90), S94 (≠ A91), G95 (= G92), L116 (≠ T113), T141 (≠ S139), Y157 (= Y153), K161 (= K157), P187 (= P183), G188 (= G184), G189 (≠ P185), T190 (≠ V186), T192 (= T188), M194 (≠ L190)
1ybvA Structure of trihydroxynaphthalene reductase in complex with NADPH and an active site inhibitor (see paper)
35% identity, 96% coverage: 8:243/245 of query aligns to 19:267/270 of 1ybvA
- active site: G27 (= G16), S151 (= S139), H162 (≠ Y150), Y165 (= Y153), K169 (= K157), Y210 (vs. gap)
- binding 5-methyl-1,2,4-triazolo[3,4-b]benzothiazole: S151 (= S139), Y165 (= Y153), G197 (≠ P185), M202 (≠ L190), Y203 (≠ F191), Y210 (vs. gap), W230 (≠ L206)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G23 (= G12), R26 (= R15), G27 (= G16), I28 (= I17), A48 (= A37), N49 (≠ S38), S50 (≠ N39), N74 (≠ D63), V75 (= V64), N101 (≠ S90), S102 (≠ A91), G103 (= G92), M149 (≠ L138), S151 (= S139), K169 (= K157), P195 (= P183), G197 (≠ P185), I198 (≠ V186), T200 (= T188), M202 (≠ L190)
1g0oC Structure of trihydroxynaphthalene reductase in complex with NADPH and pyroquilon (see paper)
35% identity, 96% coverage: 8:243/245 of query aligns to 30:278/281 of 1g0oC
- active site: G38 (= G16), S162 (= S139), H173 (≠ Y150), Y176 (= Y153), K180 (= K157), Y221 (vs. gap)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G34 (= G12), R37 (= R15), G38 (= G16), I39 (= I17), A59 (= A37), N60 (≠ S38), S61 (≠ N39), N85 (≠ D63), V86 (= V64), N112 (≠ S90), S113 (≠ A91), G114 (= G92), M160 (≠ L138), Y176 (= Y153), K180 (= K157), P206 (= P183), G208 (≠ P185), I209 (≠ V186), T211 (= T188), M213 (≠ L190)
- binding pyroquilon: S162 (= S139), I163 (≠ T140), Y176 (= Y153), G208 (≠ P185), Y221 (vs. gap)
1g0nA Structure of trihydroxynaphthalene reductase in complex with NADPH and 4,5,6,7-tetrachloro-phthalide (see paper)
35% identity, 96% coverage: 8:243/245 of query aligns to 22:270/273 of 1g0nA
- active site: G30 (= G16), S154 (= S139), H165 (≠ Y150), Y168 (= Y153), K172 (= K157)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G26 (= G12), R29 (= R15), G30 (= G16), I31 (= I17), A51 (= A37), N52 (≠ S38), S53 (≠ N39), V78 (= V64), N104 (≠ S90), S105 (≠ A91), G106 (= G92), I127 (≠ T113), M152 (≠ L138), Y168 (= Y153), K172 (= K157), P198 (= P183), G200 (≠ P185), I201 (≠ V186), T203 (= T188), M205 (≠ L190)
- binding 4,5,6,7-tetrachloro-phthalide: S154 (= S139), Y168 (= Y153), G200 (≠ P185), M205 (≠ L190), Y206 (≠ F191), C210 (vs. gap), Y213 (vs. gap), W233 (≠ L206)
1dohA Structure of trihydroxynaphthalene reductase in complex with NADPH and 4-nitro-inden-1-one (see paper)
35% identity, 96% coverage: 8:243/245 of query aligns to 22:270/273 of 1dohA
- active site: G30 (= G16), S154 (= S139), H165 (≠ Y150), Y168 (= Y153), K172 (= K157), Y213 (vs. gap)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G26 (= G12), R29 (= R15), G30 (= G16), I31 (= I17), A51 (= A37), N52 (≠ S38), S53 (≠ N39), N77 (≠ D63), V78 (= V64), N104 (≠ S90), S105 (≠ A91), G106 (= G92), M152 (≠ L138), Y168 (= Y153), K172 (= K157), P198 (= P183), G200 (≠ P185), I201 (≠ V186), T203 (= T188), M205 (≠ L190)
- binding 4-nitro-inden-1-one: Y168 (= Y153), G200 (≠ P185), Y206 (≠ F191), C210 (vs. gap), Y213 (vs. gap)
Q12634 Tetrahydroxynaphthalene reductase; T4HN reductase; THNR; EC 1.1.1.252 from Pyricularia oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Magnaporthe oryzae) (see 2 papers)
36% identity, 96% coverage: 8:243/245 of query aligns to 32:280/283 of Q12634
- 39:63 (vs. 15:39, 52% identical) binding
- Y178 (= Y153) active site, Proton acceptor
7nm8AAA Antimycin pathway standalone ketoreductase, AntM (see paper)
38% identity, 97% coverage: 6:243/245 of query aligns to 7:247/251 of 7nm8AAA
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G13 (= G12), S15 (= S14), R16 (= R15), G17 (= G16), I18 (= I17), H36 (≠ N35), Y37 (= Y36), G38 (≠ A37), H39 (≠ S38), L65 (≠ V64), N97 (≠ S90), G99 (= G92), S147 (≠ T140), Y160 (= Y153), K164 (= K157), G191 (= G184), T193 (≠ V186), T195 (= T188)
P73574 3-oxoacyl-[acyl-carrier-protein] reductase; 3-ketoacyl-acyl carrier protein reductase; EC 1.1.1.100 from Synechocystis sp. (strain PCC 6803 / Kazusa) (see paper)
41% identity, 98% coverage: 4:244/245 of query aligns to 5:244/247 of P73574
- A14 (≠ G13) mutation to G: 4.2-fold increase in activity on acetoacetyl-CoA.
- P151 (= P148) mutation to F: 2.7-fold increase in activity on acetoacetyl-CoA.; mutation to V: 5.7-fold increase in activity on acetoacetyl-CoA.
- K160 (= K157) mutation to A: Almost no activity on acetoacetyl-CoA.
- F188 (≠ P185) mutation to Y: 3.3-fold increase in activity on acetoacetyl-CoA.
- N198 (≠ K195) mutation to R: 3.5-fold increase in activity on acetoacetyl-CoA.
1edoA The x-ray structure of beta-keto acyl carrier protein reductase from brassica napus complexed with NADP+ (see paper)
39% identity, 97% coverage: 9:245/245 of query aligns to 5:243/244 of 1edoA
- active site: G12 (= G16), S138 (≠ T140), Y151 (= Y153), K155 (= K157)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), S10 (= S14), R11 (= R15), I13 (= I17), N31 (= N35), Y32 (= Y36), A33 (= A37), R34 (≠ S38), S35 (≠ N39), D59 (= D63), V60 (= V64), N86 (≠ S90), A87 (= A91), S138 (≠ T140), Y151 (= Y153), K155 (= K157), P181 (= P183), G182 (= G184), I184 (≠ V186), S186 (≠ T188), M188 (≠ L190)
4dmmB 3-oxoacyl-[acyl-carrier-protein] reductase from synechococcus elongatus pcc 7942 in complex with NADP
46% identity, 98% coverage: 4:244/245 of query aligns to 4:237/240 of 4dmmB
- active site: G16 (= G16), S142 (≠ T140), Q152 (≠ Y150), Y155 (= Y153), K159 (= K157)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G12), S14 (= S14), R15 (= R15), G16 (= G16), I17 (= I17), A37 (= A37), S38 (= S38), S39 (≠ N39), A62 (= A62), D63 (= D63), V64 (= V64), N90 (≠ S90), A91 (= A91), L113 (≠ T113), I140 (≠ L138), S142 (≠ T140), Y155 (= Y153), K159 (= K157), P185 (= P183), G186 (= G184), I188 (≠ V186), T190 (= T188), M192 (≠ L190)
5wuwA Serratia marcescens short-chain dehydrogenase/reductase f98l/f202l mutant (see paper)
37% identity, 97% coverage: 8:245/245 of query aligns to 8:244/245 of 5wuwA
- active site: G16 (= G16), S140 (= S139), Y154 (= Y153), L161 (≠ V160)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G12), R15 (= R15), I17 (= I17), Y36 (= Y36), A37 (= A37), A38 (≠ S38), D63 (= D63), S64 (≠ V64), N90 (≠ S90), A91 (= A91), G92 (= G92), Y154 (= Y153), K158 (= K157), G185 (= G184), P186 (= P185), V187 (= V186)
Query Sequence
>8502147 DvMF_2858 short-chain dehydrogenase/reductase SDR (RefSeq)
METTARNAIVTGGSRGIGRAIALRLAQDGFCVVVNYASNAPAALEVVDAIARTGGQAVAV
PADVGETGDVEGLFAAAHDAFGQVGVVVNSAGIMPMLPIAGGDTAAFEKVLRTNLTGTFN
VLSRAANALTAGGRIIALSTSVIAKPFPGYGPYIASKAGVEGLVRVLANELRGRSITVNA
VAPGPVATDLFLNGKTEEQIAAIGKLAPLERLGTPEEIAGVVSFLVGPEGGWINAQVVRV
NGGFA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory