SitesBLAST

C26 (≠ R37) mutation to A: Lower ATPase activity and transport efficiency.
F27 (= F38) mutation to L: Lower ATPase activity and transport efficiency.
F45 (= F56) mutation to L: Lower ATPase activity and transport efficiency.
C54 (= C65) mutation to T: Loss of ATPase activity and transport.
L60 (= L71) mutation to F: Lower ATPase activity and transport efficiency.
L76 (≠ I87) mutation to P: Lower ATPase activity and transport efficiency.
V135 (= V146) mutation to M: Loss of ATPase activity and transport.
D172 (= D183) mutation to N: Loss of ATPase activity and transport.
C276 (≠ F287) mutation to A: Lower ATPase activity and transport efficiency.
E297 (= E308) mutation E->K,D: Lower ATPase activity and transport efficiency.; mutation to Q: Loss of ATPase activity and transport.

2d62A Crystal structure of multiple sugar binding transport atp- binding protein
48% identity, 56% coverage: 27:260/418 of query aligns to 5:244/375 of 2d62A

query
sites
2d62A

A

A

D

E

L

V

S

K

V

L

T

I

R

N

L

I

V

W

K

K

R

R

F

F

E

G

K

D

F

V

T

T

A

A

V

V

N

K

D

D

V

L

S

S

F

L

E

E

V

I

E

K

Q

D

G

G

R

E

F

F

F

L

S

V

I

L

L

L

G

G

P
|
P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
|
T

L

T

L

L

R

R

M

M

I

I

A

A

G

G

F

L

E

E

S

E

P

P

D

T

S

R

G

G

V

Q

I

I

A

Y

I

I

R

E

G

D

R

N

D

L

M

V

A

A

G

D

-

P

-

E

-

K

-

G

-

V

-

F

I

V

A

P

P

P

N

K

R

E

R

R

P

D

V

V

N

A

L

M

I

V

F

F

Q

Q

H

S

L

Y

A

A

L

L

F

Y

P

P

M

H

M

M

S

T

V

V

A

Y

E

D

N

N

V

I

A

A

F

F

G

P

L

L

K

K

R

L

R

R

G

K

M

V

A

P

G

K

G

Q

E

E

I

I

S

D

R

K

R

R

V

V

Q

R

D

E

V

V

L

A

E

E

R

M

V

L

G

G

L

L

P

T

G

E

Y

L

G

L

V

N

K

R

M

K

P

P

A

R

Q

E

L

L

S

S

G

G

Q

Q

K

R

Q

Q

R

R

V

V

A

A

I

L

A

G

R

R

C

A

L

I

V

I

L

R

E

R

P

P

A

K

V

V

L

F

L

L

L

M

D

D

E

E

P

P

L

L

G

S

A

N

L

L

D

D

L

A

K

K

L

L

R

R

E

V

Q

K

M

M

K

R

V

A

E

E

L

L

K

K

T

K

L

L

Q

Q

A

R

E

Q

V

L

G

G

T

V

T

T

F

T

V

I

Y

Y

I

V

T

T

H

H

D

D

Q

Q

S

V

E

E

A

A

L

M

V

T

M

M

S

G

D

D

H

R

V

I

A

A

V

V

M

M

N

N

A

K

G

G

R

E

F

L

E

Q

Q

Q

V

V

D

G

T

T

P

P

R

D

N

E

L

V

Y

Y

R

Y

R

K

P

P

A

V

S

N

A

T

F

F

V

V

A

A

G

G

F

F

V

I

G

G

binding pyrophosphate 2-: P40 (= P62), S41 (= S63), G42 (= G64), C43 (= C65), G44 (= G66), K45 (= K67), T46 (= T68), T47 (= T69)

1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
44% identity, 69% coverage: 27:313/418 of query aligns to 5:281/353 of 1vciA

query
sites
1vciA

A

V

D

E

L

V

S

K

V

L

T

E

R

N

L

L

V

T

K

K

R

R

F
|
F

E

G

K

N

F
|
F

T

T

A

A

V

V

N

N

D

K

V

L

S

N

F

L

E

T

V

I

E

K

Q

D

G

G

R

E

F

F

F

L

S

V

I

L

L

L

G

G

P
|
P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
|
T

L

T

L

L

R

R

M

M

I

I

A

A

G

G

F

L

E

E

S

E

P

P

D

T

S

E

G

G

V

R

I

I

A

Y

I

F

R

G

G

D

R

R

D

D

M

V

A

T

G

Y

I

L

A

P

P

P

N

K

R

D

R

R

P

N

V

I

N

S

L

M

I

V

F

F

Q

Q

H

H

L

-

A

-

L

-

F

-

P

-

M

-

M

M

S

T

V

V

A

Y

E

E

N

N

V

I

A

A

F

F

G

P

L

L

K

K

R

K

R

-

G

-

M

F

A

P

G

K

G

D

E

E

I

I

S

D

R

K

R

R

V

V

Q

R

D

W

V

A

L

A

E

E

R

L

V

L

G

Q

L

I

P

E

G

E

Y

L

G

L

V

N

K

R

M

Y

P

P

A

A

Q

Q

L

L

S

S

G

G

Q

Q

K

R

Q

Q

R

R

V

V

A

A

I

V

A

A

R

R

C

A

L

I

V

V

L

V

E

E

P

P

A

D

V

V

L

L

L

M

D

D

E

E

P

P

L

L

G

S

A

N

L

L

D

D

L

A

K

K

L

L

R

R

E

V

Q

A

M

M

K

R

V

A

E

E

L

I

K

K

T

K

L

L

Q

Q

A

Q

E

K

V

L

G

K

T

V

T

T

F

T

V

I

Y

Y

I

V

T

T

H

H

D

D

Q

Q

S

V

E

E

A

A

L

M

V

T

M

M

S

G

D

D

H

R

V

I

A

A

V

V

M

M

N

N

A

R

G

G

R

Q

F

L

E

L

Q

Q

V

I

D

G

T

S

P

P

R

T

N

E

L

V

Y

Y

R

L

R

R

P

P

A

N

S

S

A

V

F

F

V

V

A

A

G

T

F

F

V

I

G

G

-

A

-

P

E

E

T

M

N

N

V

I

W

-

S

-

G

-

T

-

L

L

E

E

E

V

A

S

T

V

G

G

D

D

A

G

G

Y

L

L

V

-

R

-

T

-

D

-

E

E

G

G

A

R

V

G

F

F

R

R

A

I

R

E

V

L

-

P

A

Q

A

M

G

D

L

L

A

L

K

K

-

D

-

Y

-

V

G

G

T

K

R

T

V

V

D

L

M

F

F

G

I

I

R

R

P

P

E

E

A

H

V

M

L

T

I

V

D

E

binding adenosine-5'-triphosphate: F16 (= F38), F19 (= F41), P40 (= P62), S41 (= S63), G42 (= G64), C43 (= C65), G44 (= G66), K45 (= K67), T46 (= T68), T47 (= T69)

1g291 Malk (see paper)
49% identity, 54% coverage: 36:260/418 of query aligns to 11:241/372 of 1g291

query
sites
1g291

K

K

R

V

F

F

E

G

K

E

F

V

T

T

A

A

V

V

N

R

D

E

V

M

S

S

F

L

E

E

V

V

E

K

Q

D

G

G

R

E

F

F

F

M

S

I

I

L

L

L

G

G

P
|
P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
|
T

L

T

L

L

R

R

M

M

I

I

A

A

G

G

F

L

E

E

S

E

P

P

D

S

S

R

G

G

V

Q

I

I

A

Y

I

I

R

G

G

D

R

K

D

L

M

V

A

A

G
x
D

-

P

-
x
E

-
x
K

-

G

-

I

-

F

I

V

A

P

P

P

N
x
K

R
x
D

R

R

P

D

V

I

N

A

L

M

I

V

F

F

Q

Q

H

S

L

Y

A

A

L

L

F

Y

P

P

M

H

M

M

S

T

V

V

A

Y

E

D

N

N

V

I

A

A

F

F

G

P

L

L

K

K

R

L

R

R

G

K

M

V

A

P

G

R

G

Q

E

E

I

I

S

D

R

Q

R

R

V

V

Q

R

D

E

V

V

L

A

E

E

R

L

V

L

G

G

L

L

P

T

G

E

Y

L

G

L

V

N

K

R

M

K

P

P

A

R

Q

E

L

L

S

S

G

G

Q

Q

K

R

Q

Q

R

R

V

V

A

A

I

L

A

G

R

R

C

A

L

I

V

V

L

R

E

K

P

P

A

Q

V

V

L

F

L

L

L

M

D

D

E

E

P

P

L

L

G

S

A

N

L

L

D

D

L

A

K

K

L

L

R

R

E

V

Q

R

M

M

K

R

V

A

E

E

L

L

K

K

T

K

L

L

Q

Q

A

R

E

Q

V

L

G

G

T

V

T

T

F

T

V

I

Y

Y

I

V

T

T

H

H

D

D

Q

Q

S

V

E

E

A

A

L

M

V

T

M

M

S

G

D

D

H

R

V

I

A

A

V

V

M

M

N

N

A

R

G

G

R

V

F

L

E

Q

Q

Q

V

V

D

G

T

S

P

P

R

D

N

E

L

V

Y
|
Y

R
x
D

R

K

P

P

A

A

S

N

A

T

F

F

V

V

A

A

G

G

F

F

V

I

G

G

binding magnesium ion: D69 (≠ G94), E71 (vs. gap), K72 (vs. gap), K79 (≠ N98), D80 (≠ R99), Y228 (= Y247), D229 (≠ R248)
binding pyrophosphate 2-: P37 (= P62), S38 (= S63), G39 (= G64), C40 (= C65), G41 (= G66), K42 (= K67), T43 (= T68), T44 (= T69)

Sites not aligning to the query:

binding magnesium ion: 292, 293, 341, 342, 359

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
46% identity, 56% coverage: 27:260/418 of query aligns to 1:234/374 of 2awnB

query
sites
2awnB

A

A

D

S

L

V

S

Q

V

L

T

Q

R

N

L

V

V

T

K

K

R

A

F
x
W

E

G

K

E

F

V

T

V

A
x
V

V

S

N

K

D

D

V

I

S

N

F

L

E

D

V

I

E

H

Q

E

G

G

R

E

F

F

F

V

S

V

I

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

M

M

I

I

A

A

G

G

F

L

E

E

S

T

P

I

D

T

S

S

G

G

V

D

I

L

A

F

I

I

R

G

G

E

R

K

D

R

M

M

A

N

G

D

I

T

A

P

P

P

N

A

R

E

R

R

P

G

V

V

N

G

L

M

I

V

F

F

Q

Q

H

S

L

Y

A

A

L

L

F

Y

P

P

M

H

M

L

S

S

V

V

A

A

E

E

N

N

V

M

A

S

F

F

G

G

L

L

K

K

R

L

R

A

G

G

M

A

A

K

G

K

G

E

E

V

I

I

S

N

R

Q

R

R

V

V

Q

N

D

Q

V

V

L

A

E

E

R

V

V

L

G

Q

L

L

P

A

G

H

Y

L

G

L

V

D

K

R

M

K

P

P

A

K

Q

A

L

L

S

S

G

G

Q

Q

K

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

C

T

L

L

V

V

L

A

E

E

P

P

A

S

V

V

L

F

L

L

D

D

E

E

P

P

L

L

G

S

A

N

L

L

D

D

L

A

K

A

L

L

R

R

E

V

Q

Q

M

M

K

R

V

I

E

E

L

I

K

S

T

R

L

L

Q

H

A

K

E

R

V

L

G

G

T

R

T

T

F

M

V

I

Y

Y

I

V

T

T

H

H

D

D

Q

Q

S

V

E

E

A

A

L

M

V

T

M

L

S

A

D

D

H

K

V

I

A

V

V

V

M

L

N

D

A

A

G

G

R

R

F

V

E

A

Q

Q

V

V

D

G

T

K

P

P

R

L

N

E

L

L

Y

Y

R

H

R

Y

P

P

A

A

S

D

A

R

F

F

V

V

A

A

G

G

F

F

V

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ F38), V17 (≠ A43), S37 (= S63), G38 (= G64), C39 (= C65), G40 (= G66), K41 (= K67), S42 (≠ T68), T43 (= T69)

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
46% identity, 56% coverage: 27:260/418 of query aligns to 1:234/371 of 3puyA

query
sites
3puyA

A

A

D

S

L

V

S

Q

V

L

T

Q

R

N

L

V

V

T

K

K

R

A

F
x
W

E

G

K

E

F

V

T

V

A
x
V

V

S

N

K

D

D

V

I

S

N

F

L

E

D

V

I

E

H

Q

E

G

G

R

E

F

F

F

V

S

V

I

F

L

V

G

G

P
|
P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

M

M

I

I

A

A

G

G

F

L

E

E

S

T

P

I

D

T

S

S

G

G

V

D

I

L

A

F

I

I

R

G

G

E

R

K

D

R

M

M

A

N

G

D

I

T

A

P

P

P

N

A

R

E

R

R

P

G

V

V

N

G

L

M

I

V

F

F

Q
|
Q

H

S

L

Y

A

A

L

L

F

Y

P

P

M

H

M

L

S

S

V

V

A

A

E

E

N

N

V

M

A

S

F

F

G

G

L

L

K

K

R

L

R

A

G

G

M

A

A

K

G

K

G

E

E

V

I

I

S

N

R

Q

R

R

V

V

Q

N

D

Q

V

V

L

A

E

E

R

V

V

L

G

Q

L

L

P

A

G

H

Y

L

G

L

V

D

K
x
R

M

K

P

P

A

K

Q
x
A

L

L

S
|
S

G
|
G

Q
|
Q

K

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

C

T

L

L

V

V

L

A

E

E

P

P

A

S

V

V

L

F

L

L

D
|
D

E
|
E

P

P

L

L

G

S

A

N

L

L

D

D

L

A

K

A

L

L

R

R

E

V

Q

Q

M

M

K

R

V

I

E

E

L

I

K

S

T

R

L

L

Q

H

A

K

E

R

V

L

G

G

T

R

T

T

F

M

V

I

Y

Y

I

V

T

T

H
|
H

D

D

Q

Q

S

V

E

E

A

A

L

M

V

T

M

L

S

A

D

D

H

K

V

I

A

V

V

V

M

L

N

D

A

A

G

G

R

R

F

V

E

A

Q

Q

V

V

D

G

T

K

P

P

R

L

N

E

L

L

Y

Y

R

H

R

Y

P

P

A

A

S

D

A

R

F

F

V

V

A

A

G

G

F

F

V

I

G

G

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F38), V17 (≠ A43), P36 (= P62), S37 (= S63), G38 (= G64), C39 (= C65), G40 (= G66), K41 (= K67), S42 (≠ T68), T43 (= T69), Q81 (= Q107), R128 (≠ K154), A132 (≠ Q158), S134 (= S160), G135 (= G161), G136 (= G162), Q137 (= Q163), E158 (= E184), H191 (= H217)
binding magnesium ion: S42 (≠ T68), Q81 (= Q107), D157 (= D183)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
46% identity, 56% coverage: 27:260/418 of query aligns to 1:234/371 of 3puxA

query
sites
3puxA

A

A

D

S

L

V

S

Q

V

L

T

Q

R

N

L

V

V

T

K

K

R

A

F
x
W

E

G

K

E

F

V

T

V

A
x
V

V

S

N

K

D

D

V

I

S

N

F

L

E

D

V

I

E

H

Q

E

G

G

R

E

F

F

F

V

S

V

I

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

M

M

I

I

A

A

G

G

F

L

E

E

S

T

P

I

D

T

S

S

G

G

V

D

I

L

A

F

I

I

R

G

G

E

R

K

D

R

M

M

A

N

G

D

I

T

A

P

P

P

N

A

R

E

R

R

P

G

V

V

N

G

L

M

I

V

F

F

Q
|
Q

H

S

L

Y

A

A

L

L

F

Y

P

P

M

H

M

L

S

S

V

V

A

A

E

E

N

N

V

M

A

S

F

F

G

G

L

L

K

K

R

L

R

A

G

G

M

A

A

K

G

K

G

E

E

V

I

I

S

N

R

Q

R

R

V

V

Q

N

D

Q

V

V

L

A

E

E

R

V

V

L

G

Q

L

L

P

A

G

H

Y

L

G

L

V

D

K
x
R

M

K

P

P

A

K

Q
x
A

L
|
L

S
|
S

G
|
G

Q
|
Q

K

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

C

T

L

L

V

V

L

A

E

E

P

P

A

S

V

V

L

F

L

L

D
|
D

E
|
E

P

P

L

L

G

S

A

N

L

L

D

D

L

A

K

A

L

L

R

R

E

V

Q

Q

M

M

K

R

V

I

E

E

L

I

K

S

T

R

L

L

Q

H

A

K

E

R

V

L

G

G

T

R

T

T

F

M

V

I

Y

Y

I

V

T

T

H
|
H

D

D

Q

Q

S

V

E

E

A

A

L

M

V

T

M

L

S

A

D

D

H

K

V

I

A

V

V

V

M

L

N

D

A

A

G

G

R

R

F

V

E

A

Q

Q

V

V

D

G

T

K

P

P

R

L

N

E

L

L

Y

Y

R

H

R

Y

P

P

A

A

S

D

A

R

F

F

V

V

A

A

G

G

F

F

V

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ F38), V17 (≠ A43), S37 (= S63), G38 (= G64), C39 (= C65), G40 (= G66), K41 (= K67), S42 (≠ T68), T43 (= T69), R128 (≠ K154), A132 (≠ Q158), L133 (= L159), S134 (= S160), Q137 (= Q163)
binding beryllium trifluoride ion: S37 (= S63), G38 (= G64), K41 (= K67), Q81 (= Q107), S134 (= S160), G135 (= G161), G136 (= G162), E158 (= E184), H191 (= H217)
binding magnesium ion: S42 (≠ T68), Q81 (= Q107), D157 (= D183)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
46% identity, 56% coverage: 27:260/418 of query aligns to 1:234/371 of 3puwA

query
sites
3puwA

A

A

D

S

L

V

S

Q

V

L

T

Q

R

N

L

V

V

T

K

K

R

A

F
x
W

E

G

K

E

F

V

T

V

A
x
V

V

S

N

K

D

D

V

I

S

N

F

L

E

D

V

I

E

H

Q

E

G

G

R

E

F

F

F

V

S

V

I

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

M

M

I

I

A

A

G

G

F

L

E

E

S

T

P

I

D

T

S

S

G

G

V

D

I

L

A

F

I

I

R

G

G

E

R

K

D

R

M

M

A

N

G

D

I

T

A

P

P

P

N

A

R

E

R

R

P

G

V

V

N

G

L

M

I

V

F

F

Q
|
Q

H

S

L

Y

A

A

L

L

F

Y

P

P

M

H

M

L

S

S

V

V

A

A

E

E

N

N

V

M

A

S

F

F

G

G

L

L

K

K

R

L

R

A

G

G

M

A

A

K

G

K

G

E

E

V

I

I

S

N

R

Q

R

R

V

V

Q

N

D

Q

V

V

L

A

E

E

R

V

V

L

G

Q

L

L

P

A

G

H

Y

L

G

L

V

D

K
x
R

M

K

P

P

A

K

Q
x
A

L

L

S
|
S

G
|
G

Q
|
Q

K

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

C

T

L

L

V

V

L

A

E

E

P

P

A

S

V

V

L

F

L

L

D

D

E
|
E

P

P

L

L

G

S

A
x
N

L

L

D

D

L

A

K

A

L

L

R

R

E

V

Q

Q

M

M

K

R

V

I

E

E

L

I

K

S

T

R

L

L

Q

H

A

K

E

R

V

L

G

G

T

R

T

T

F

M

V

I

Y

Y

I

V

T

T

H
|
H

D

D

Q

Q

S

V

E

E

A

A

L

M

V

T

M

L

S

A

D

D

H

K

V

I

A

V

V

V

M

L

N

D

A

A

G

G

R

R

F

V

E

A

Q

Q

V

V

D

G

T

K

P

P

R

L

N

E

L

L

Y

Y

R

H

R

Y

P

P

A

A

S

D

A

R

F

F

V

V

A

A

G

G

F

F

V

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ F38), V17 (≠ A43), S37 (= S63), G38 (= G64), C39 (= C65), G40 (= G66), K41 (= K67), S42 (≠ T68), T43 (= T69), R128 (≠ K154), A132 (≠ Q158), S134 (= S160), Q137 (= Q163)
binding tetrafluoroaluminate ion: S37 (= S63), G38 (= G64), K41 (= K67), Q81 (= Q107), S134 (= S160), G135 (= G161), G136 (= G162), E158 (= E184), N162 (≠ A188), H191 (= H217)
binding magnesium ion: S42 (≠ T68), Q81 (= Q107)

2awoA Crystal structure of the adp-mg-bound e. Coli malk (crystallized with adp-mg) (see paper)
46% identity, 56% coverage: 27:260/418 of query aligns to 1:234/372 of 2awoA

query
sites
2awoA

A

A

D

S

L

V

S

Q

V

L

T

Q

R

N

L

V

V

T

K

K

R

A

F
x
W

E

G

K

E

F

V

T

V

A
x
V

V

S

N

K

D

D

V

I

S

N

F

L

E

D

V

I

E

H

Q

E

G

G

R

E

F

F

F

V

S

V

I

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

M

M

I

I

A

A

G

G

F

L

E

E

S

T

P

I

D

T

S

S

G

G

V

D

I

L

A

F

I

I

R

G

G

E

R

K

D

R

M

M

A

N

G

D

I

T

A

P

P

P

N

A

R

E

R

R

P

G

V

V

N

G

L

M

I

V

F

F

Q

Q

H

S

L

Y

A

A

L

L

F

Y

P

P

M

H

M

L

S

S

V

V

A

A

E

E

N

N

V

M

A

S

F

F

G

G

L

L

K

K

R

L

R

A

G

G

M

A

A

K

G

K

G

E

E

V

I

I

S

N

R

Q

R

R

V

V

Q

N

D

Q

V

V

L

A

E

E

R

V

V

L

G

Q

L

L

P

A

G

H

Y

L

G

L

V

D

K

R

M

K

P

P

A

K

Q

A

L

L

S

S

G

G

Q

Q

K

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

C

T

L

L

V

V

L

A

E

E

P

P

A

S

V

V

L

F

L

L

D
|
D

E

E

P

P

L

L

G

S

A

N

L

L

D

D

L

A

K

A

L

L

R

R

E

V

Q

Q

M

M

K

R

V

I

E

E

L

I

K

S

T

R

L

L

Q

H

A

K

E

R

V

L

G

G

T

R

T

T

F

M

V

I

Y

Y

I

V

T

T

H

H

D

D

Q

Q

S

V

E

E

A

A

L

M

V

T

M

L

S

A

D

D

H

K

V

I

A

V

V

V

M

L

N

D

A

A

G

G

R

R

F

V

E

A

Q

Q

V

V

D

G

T

K

P

P

R

L

N

E

L

L

Y

Y

R

H

R

Y

P

P

A

A

S

D

A

R

F

F

V

V

A

A

G

G

F

F

V

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ F38), V17 (≠ A43), S37 (= S63), G38 (= G64), C39 (= C65), G40 (= G66), K41 (= K67), S42 (≠ T68), T43 (= T69)
binding magnesium ion: S42 (≠ T68), D157 (= D183)

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
46% identity, 56% coverage: 27:260/418 of query aligns to 2:235/371 of P68187

query
sites
P68187

A

A

D

S

L

V

S

Q

V

L

T

Q

R

N

L

V

V

T

K

K

R

A

F

W

E

G

K

E

F

V

T

V

A

V

V

S

N

K

D

D

V

I

S

N

F

L

E

D

V

I

E

H

Q

E

G

G

R

E

F

F

F

V

S

V

I

F

L

V

G

G

P

P

S

S

G

G

C

C

G

G

K

K

T

S

T

T

L

L

R

R

M

M

I

I

A

A

G

G

F

L

E

E

S

T

P

I

D

T

S

S

G

G

V

D

I

L

A

F

I

I

R

G

G

E

R

K

D

R

M

M

A

N

G

D

I

T

A

P

P

P

N

A

R

E

R

R

P

G

V

V

N

G

L

M

I

V

F

F

Q

Q

H

S

L

Y

A
|
A

L

L

F

Y

P

P

M

H

M

L

S

S

V

V

A

A

E

E

N

N

V

M

A

S

F

F

G

G

L

L

K

K

R

L

R

A

G

G

M

A

A
x
K

G

K

G

E

E

V

I

I

S

N

R

Q

R

R

V
|
V

Q

N

D

Q

V
|
V

L

A

E
|
E

R

V

V

L

G

Q

L

L

P
x
A

G

H

Y

L

G

L

V

D

K

R

M

K

P

P

A

K

Q

A

L

L

S

S

G

G

G
|
G

Q

Q

K

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

C

T

L

L

V

V

L

A

E

E

P

P

A

S

V

V

L

F

L

L

D
|
D

E

E

P

P

L

L

G

S

A

N

L

L

D

D

L

A

K

A

L

L

R

R

E

V

Q

Q

M

M

K

R

V

I

E

E

L

I

K

S

T

R

L

L

Q

H

A

K

E

R

V

L

G

G

T

R

T

T

F

M

V

I

Y

Y

I

V

T

T

H

H

D

D

Q

Q

S

V

E

E

A

A

L

M

V

T

M

L

S

A

D

D

H

K

V

I

A

V

V

V

M

L

N

D

A

A

G

G

R

R

F

V

E

A

Q

Q

V

V

D

G

T

K

P

P

R

L

N

E

L

L

Y

Y

R

H

R

Y

P

P

A

A

S

D

A
x
R

F

F

V

V

A

A

G

G

F

F

V

I

G

G

A85 (= A110) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ A131) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (= V139) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (= V142) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (= E144) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ P149) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G162) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D183) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
R228 (≠ A253) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.

Sites not aligning to the query:

241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
46% identity, 56% coverage: 29:260/418 of query aligns to 1:232/367 of 1q12A

query
sites
1q12A

L

V

S

Q

V

L

T

Q

R

N

L

V

V

T

K

K

R

A

F
x
W

E

G

K

E

F

V

T

V

A
x
V

V

S

N

K

D

D

V

I

S

N

F

L

E

D

V

I

E

H

Q

E

G

G

R

E

F

F

F

V

S

V

I

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

M

M

I

I

A

A

G

G

F

L

E

E

S

T

P

I

D

T

S

S

G

G

V

D

I

L

A

F

I

I

R

G

G

E

R

K

D

R

M

M

A

N

G

D

I

T

A

P

P

P

N

A

R

E

R

R

P

G

V

V

N

G

L

M

I

V

F

F

Q

Q

H

S

L

Y

A

A

L

L

F

Y

P

P

M

H

M

L

S

S

V

V

A

A

E

E

N

N

V

M

A

S

F

F

G

G

L

L

K

K

R

L

R

A

G

G

M

A

A

K

G

K

G

E

E

V

I

I

S

N

R

Q

R

R

V

V

Q

N

D

Q

V

V

L

A

E

E

R

V

V

L

G

Q

L

L

P

A

G

H

Y

L

G

L

V

D

K
x
R

M

K

P

P

A
x
K

Q
x
A

L
|
L

S
|
S

G
|
G

Q
|
Q

K

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

C

T

L

L

V

V

L

A

E

E

P

P

A

S

V

V

L

F

L

L

D

D

E

E

P

P

L

L

G

S

A
x
N

L

L

D

D

L

A

K

A

L

L

R

R

E

V

Q

Q

M

M

K

R

V

I

E

E

L

I

K

S

T

R

L

L

Q

H

A

K

E

R

V

L

G

G

T

R

T

T

F

M

V

I

Y

Y

I

V

T

T

H
|
H

D

D

Q

Q

S

V

E

E

A

A

L

M

V

T

M

L

S

A

D

D

H

K

V

I

A

V

V

V

M

L

N

D

A

A

G

G

R

R

F

V

E

A

Q

Q

V

V

D

G

T

K

P

P

R

L

N

E

L

L

Y

Y

R

H

R

Y

P

P

A

A

S

D

A

R

F

F

V

V

A

A

G

G

F

F

V

I

G

G

binding adenosine-5'-triphosphate: W10 (≠ F38), V15 (≠ A43), S35 (= S63), G36 (= G64), C37 (= C65), G38 (= G66), K39 (= K67), S40 (≠ T68), T41 (= T69), R126 (≠ K154), K129 (≠ A157), A130 (≠ Q158), L131 (= L159), S132 (= S160), G133 (= G161), G134 (= G162), Q135 (= Q163), N160 (≠ A188), H189 (= H217)

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
40% identity, 68% coverage: 27:311/418 of query aligns to 2:289/369 of P19566

query
sites
P19566

A

A

D

S

L

V

S

Q

V

L

T

R

R

N

L

V

V

T

K

K

R

A

F

W

E

G

K

D

F

V

T

V

A

V

V

S

N

K

D

D

V

I

S

N

F

L

E

D

V

I

E

H

Q

D

G

G

R

E

F

F

F

V

S

V

I

F

L

V

G

G

P

P

S

S

G

G

C

C

G

G

K

K

T

S

T

T

L

L

R

R

M

M

I

I

A

A

G

G

F

L

E

E

S

T

P

I

D

T

S

S

G

G

V

D

I

L

A

F

I

I

R

G

G

E

R

T

D

R

M

M

A

N

G

D

I

I

A

P

P

P

N

A

R

E

R

R

P

G

V

V

N

G

L

M

I

V

F

F

Q

Q

H

S

L

Y

A

A

L
|
L

F

Y

P

P

M

H

M

L

S

S

V

V

A

A

E

E

N

N

V

M

A

S

F

F

G

G

L

L

K

K

R

L

R

A

G

G

M

A

A

K

G

K

G

E

E

V

I

M

S

N

R

Q

R

R

V

V

Q

N

D

Q

V

V

L

A

E

E

R

V

V

L

G

Q

L

L

P

A

G

H

Y

L

G

L

V

E

K

R

M

K

P

P

A

K

Q

A

L

L

S

S

G

G

Q

Q

K

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

C

T

L

L

V

V

L

A

E

E

P

P

A

R

V

V

L

F

L

L

D

D

E

E

P
|
P

L

L

G

S

A

N

L

L

D
|
D

L

A

K

A

L

L

R

R

E

V

Q

Q

M

M

K

R

V

I

E

E

L

I

K

S

T

R

L

L

Q

H

A

K

E

R

V

L

G

G

T

R

T

T

F

M

V

I

Y

Y

I

V

T

T

H

H

D

D

Q

Q

S

V

E

E

A

A

L

M

V

T

M

L

S

A

D

D

H

K

V

I

A

V

V

V

M

L

N

D

A

A

G

G

R

R

F

V

E

A

Q

Q

V

V

D

G

T

K

P

P

R

L

N

E

L

L

Y

Y

R

H

R

Y

P

P

A

A

S

D

A

R

F

F

V

V

A

A

G

G

F

F

V

I

G

G

E

S

-

P

-

K

-

M

-

N

-

F

-

L

-

P

T

V

N

K

V

V

W

T

S

A

G

T

T

A

L

I

E

E

E

Q

A

V

T

Q

G

V

D

E

A

L

G

-

L

-

V

-

R

-

T

P

D

N

E

R

G

Q

A

Q

V

I

F

W

R

L

A

P

R

V

V

E

A

S

A

R

G

G

L

V

A

Q

K

V

G

G

T

A

R

N

V

M

D

S

M

L

F

G

I

I

R

R

P

P

E

E

A

H

V

L

L

L

L86 (= L111) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P185) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D190) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
47% identity, 52% coverage: 42:260/418 of query aligns to 18:236/393 of P9WQI3

query
sites
P9WQI3

T

T

A

A

V

V

N

R

D

D

V

L

S

N

F

L

E

T

V

I

E

A

Q

D

G

G

R

E

F

F

F

L

S

I

I

L

L

V

G

G

P

P

S

S

G

G

C

C

G

G

K

K

T

T

L

T

L

L

R

N

M

M

I

I

A

A

G

G

F

L

E

E

S

D

P

I

D

S

S

S

G

G

V

E

I

L

A

R

I

I

R

A

G

G

R

E

D

R

M

V

A

N

G

E

I

K

A

A

P

P

N

K

R

D

R

R

P

D

V

I

N

A

L

M

I

V

F

F

Q

Q

H

S

L

Y

A

A

L

L

F

Y

P

P

M

H

M

M

S

T

V

V

A

R

E

Q

N

N

V

I

A

A

F

F

G

P

L

L

K

T

R

L

R

A

G

K

M

M

A

R

G

K

G

A

E

D

I

I

S

A

R

Q

R

K

V

V

Q

S

D

E

V

T

L

A

E

K

R

I

V

L

G

D

L

L

P

T

G

N

Y

L

G

L

V

D

K

R

M

K

P

P

A

S

Q

Q

L

L

S

S

G

G

Q

Q

K

R

Q

Q

R

R

V

V

A

A

I

M

A

G

R

R

C

A

L

I

V

V

L

R

E

H

P

P

A

K

V

A

L

F

L

L

L

M

D

D

E

E

P

P

L

L

G

S

A

N

L

L

D

D

L

A

K

K

L

L

R

R

E

V

Q

Q

M

M

K

R

V

G

E

E

L

I

K

A

T

Q

L

L

Q

Q

A

R

E

R

V

L

G

G

T

T

F

T

V

V

Y

Y

I

V

T

T

H
|
H

D

D

Q

Q

S

T

E

E

A

A

L

M

V

T

M

L

S

G

D

D

H

R

V

V

A

V

V

V

M

M

N

Y

A

G

G

G

R

I

F

A

E

Q

Q

Q

V

I

D

G

T

T

P

P

R

E

N

E

L

L

Y

Y

R

E

R

R

P

P

A

A

S

N

A

L

F

F

V

V

A

A

G

G

F

F

V

I

G

G

H193 (= H217) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.

7cagC Mycobacterium smegmatis lpqy-sugabc complex in the catalytic intermediate state (see paper)
44% identity, 56% coverage: 27:260/418 of query aligns to 1:232/362 of 7cagC

query
sites
7cagC

A

A

D

E

L

I

S

V

V

L

T

D

R

R

L

V

V

T

K

K

R

S

F

Y

E

P

K

-

F

-

T
x
A

A

A

V

V

N

K

D

E

V

F

S

S

F

M

E

T

V

I

E

A

Q

D

G

G

R

E

F

F

F

I

S

I

I

L

L

V

G

G

P
|
P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

T

L

L

R

N

M

M

I

I

A

A

G

G

F

L

E

E

S

E

P

I

D

T

S

S

G

G

V

E

I

L

A

R

I

I

R

G

G

G

R

E

D

R

M

V

A

N

G

E

I

K

A

A

P

P

N

K

R

D

R

R

P

D

V

I

N

A

L

M

I

V

F

F

Q
|
Q

H

S

L

Y

A

A

L

L

F

Y

P

P

M

H

M

M

S

T

V

V

A

R

E

Q

N

N

V

I

A

A

F

F

G

P

L

L

K

T

R

L

R

A

G

K

M

V

A

P

G

K

G

A

E

E

I

I

S

A

R

A

R

K

V

V

Q

E

D

E

V

T

L

A

E

K

R

I

V

L

G

D

L

L

P

S

G

E

Y
x
L

G

L

V

D

K
x
R

M

K

P

P

A

G

Q
|
Q

L

L

S
|
S

G
|
G

Q
|
Q

K

R

Q

Q

R

R

V

V

A

A

I

M

A

G

R

R

C

A

L

I

V

V

L

R

E

S

P

P

A

K

V

A

L

F

L

L

L

M

D

D

E

Q

P

P

L

L

G

S

A

N

L

L

D

D

L

A

K

K

L

L

R

R

E

V

Q

Q

M

M

K

R

V

A

E

E

L

I

K

S

T

R

L

L

Q

Q

A

D

E

R

V

L

G

G

T

T

F

T

V

V

Y

Y

I

V

T

T

H

H

D

D

Q

Q

S

T

E

E

A

A

L

M

V

T

M

L

S

G

D

D

H

R

V

V

A

V

V

V

M

M

N

L

A

A

G

G

R

E

F

V

E

Q

Q

Q

V

I

D

G

T

T

P

P

R

D

N

E

L

L

Y

Y

R

S

P

P

A

A

S

N

A

L

F

F

V

V

A

A

G

G

F

F

V

I

G

G

binding adenosine-5'-triphosphate: A14 (≠ T42), P34 (= P62), S35 (= S63), G36 (= G64), C37 (= C65), G38 (= G66), K39 (= K67), S40 (≠ T68), T41 (= T69), Q79 (= Q107), L123 (≠ Y151), R126 (≠ K154), Q130 (= Q158), S132 (= S160), G133 (= G161), G134 (= G162), Q135 (= Q163)
binding magnesium ion: S40 (≠ T68), Q79 (= Q107)

3d31A Modbc from methanosarcina acetivorans (see paper)
35% identity, 74% coverage: 29:337/418 of query aligns to 2:303/348 of 3d31A

query
sites
3d31A

L

I

S

E

V

I

T

E

R

S

L

L

V

S

K

R

R

K

F

W

E

K

K

N

F

F

T

S

A

-

V

L

N

D

D

N

V

L

S

S

F

L

E

K

V

V

E

E

Q

S

G

G

R

E

F

Y

F

F

S

V

I

I

L

L

G

G

P

P

S

T

G

G

C

A

G

G

K

K

T

T

T

L

L

F

L

L

R

E

M

L

I

I

A

A

G

G

F

F

E

H

S

V

P

P

D

D

S

S

G

G

V

R

I

I

A

L

I

L

R

D

G

G

R

K

D

D

M

V

A

T

G

D

I

L

A

S

P

P

N

E

R

K

R

H

P

D

V

I

N

A

L

F

I

V

F

Y

Q

Q

H

N

L

Y

A

S

L

L

F

F

P

P

M

H

M

M

S

N

V

V

A

K

E

K

N

N

V

L

A

E

F

F

G

G

L

M

K

R

R

M

R

K

G

K

M

I

A

K

G

D

G

-

E

-

I

-

S

P

R

K

R

R

V

V

Q

L

D

D

V

T

L

A

E

R

R

D

V

L

G

K

L

I

P

E

G

H

Y

L

G

L

V

D

K

R

M

N

P

P

A

L

Q

T

L

L

S

S

G

G

Q

E

K

Q

Q

Q

R

R

V

V

A

A

I

L

A

A

R

R

C

A

L

L

V

V

L

T

E

N

P

P

A

K

V

I

L

L

D

D

E

E

P

P

L

L

G

S

A

A

L

L

D

D

L

P

K

R

L

T

R

Q

E

E

Q

N

M

A

K

R

V

E

E

M

L

L

K

S

T

V

L

L

Q

H

A

K

E

K

V

N

G

K

T

L

T

T

F

V

V

L

Y

H

I

I

T

T

H

H

D

D

Q

Q

S

T

E

E

A

A

L

R

V

I

M

M

S

A

D

D

H

R

V

I

A

A

V

V

M

V

N

M

A

D

G

G

R

K

F

L

E

I

Q

Q

V

V

D

G

T

K

P

P

R

E

N

E

L

I

Y

F

R

E

R

K

P

P

A

V

S

E

A

G

F

R

V

V

A

A

G

S

F

F

V

V

G

G

E

F

T

E

N

N

V

V

W

L

S

K

G

G

T

R

L

V

E

-

A

I

T

S

G

A

D

E

A

Q

G

G

L

L

V

L

R

R

T

I

D

R

E

V

G

G

A

E

V

V

F

V

R

-

A

I

R

D

V

A

A

A

A

G

G

D

L

M

A

E

K

V

G

G

T

D

R

Q

V

V

D

Y

M

A

F

F

I

L

R

R

P

P

E

E

A

N

V

I

L

A

I

L

D

S

P

K

D

S

G

S

A

T

S

Q

C

S

A
x
S

T
x
I

D
x
R

G

N

A

S

T

L

D

Q

G

G

A

R

T

V

D

T

G

E

A

A

A

W

G

V

D

L

G

G

A

A

binding tungstate(vi)ion: S286 (≠ A320), I287 (≠ T321), R288 (≠ D322)

Sites not aligning to the query:

binding tungstate(vi)ion: 319, 320, 323, 340, 341, 342

2awnC Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
44% identity, 54% coverage: 37:260/418 of query aligns to 4:204/344 of 2awnC

query
sites
2awnC

R

Q

F

L

E

Q

K

N

F

V

T

T

A
x
V

V

S

N

K

D

D

V

I

S

N

F

L

E

D

V

I

E

H

Q

E

G

G

R

E

F

F

F

V

S

V

I

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

M

M

I

I

A

A

G

G

F

L

E

G

S

-

P

-

D

-

S

-

G

-

V

-

I

-

A

-

I

-

R

-

G

-

R

-

D

-

M

-

A

-

G

-

I

-

A

-

P

-

N

-

R

-

P

-

V

V

N

G

L

M

I

V

F

F

Q

Q

H

S

L

Y

A

A

L

L

F

Y

P

P

M

H

M

L

S

S

V

V

A

A

E

E

N

N

V

M

A

S

F

F

G

G

L

L

K

K

R

L

R

A

G

G

M

A

A

K

G

K

G

E

E

V

I

I

S

N

R

Q

R

R

V

V

Q

N

D

Q

V

V

L

A

E

E

R

V

V

L

G

Q

L

L

P

A

G

H

Y

L

G

L

V

D

K

R

M

K

P

P

A

K

Q

A

L

L

S

S

G

G

Q

Q

K

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

C

T

L

L

V

V

L

A

E

E

P

P

A

S

V

V

L

F

L

L

D

D

E

E

P

P

L

L

G

S

A

N

L

L

D

D

L

A

K

A

L

L

R

R

E

V

Q

Q

M

M

K

R

V

I

E

E

L

I

K

S

T

R

L

L

Q

H

A

K

E

R

V

L

G

G

T

R

T

T

F

M

V

I

Y

Y

I

V

T

T

H

H

D

D

Q

Q

S

V

E

E

A

A

L

M

V

T

M

L

S

A

D

D

H

K

V

I

A

V

V

V

M

L

N

D

A

A

G

G

R

R

F

V

E

A

Q

Q

V

V

D

G

T

K

P

P

R

L

N

E

L

L

Y

Y

R

H

R

Y

P

P

A

A

S

D

A

R

F

F

V

V

A

A

G

G

F

F

V

I

G

G

binding adenosine-5'-diphosphate: V10 (≠ A43), S30 (= S63), G31 (= G64), C32 (= C65), G33 (= G66), K34 (= K67), S35 (≠ T68), T36 (= T69)

1oxvD Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
35% identity, 68% coverage: 30:315/418 of query aligns to 8:286/353 of 1oxvD

query
sites
1oxvD

S

N

V

V

T

S

R

K

L

V

V
x
F

K

K

R

K

F

-

E

G

K

K

F
x
V

T

V

A
|
A

V

L

N

D

D

N

V

V

S

N

F

I

E

N

V

I

E

E

Q

N

G

G

R

E

F

R

F

F

S

G

I

I

L

L

G

G

P
|
P

S
|
S

G
|
G

C
x
A

G
|
G

K
|
K

T
|
T

L

F

L

M

R

R

M

I

I

I

A

A

G

G

F

L

E

D

S

V

P

P

D

S

S

T

G

G

V

E

I

L

A

Y

I

F

R

D

G

D

R

R

D

L

M

V

A

A

G

S

-

N

-

G

-

K

-

L

-

I

I

V

A

P

P

P

N

E

R

D

R

R

P

K

V

I

N

G

L

M

I

V

F

F

Q
|
Q

H

T

L

W

A

A

L

L

F

Y

P

P

M

N

M

L

S

T

V

A

A

F

E

E

N

N

V

I

A

A

F

F

G

P

L

L

K

T

R

N

R

M

G

K

M

M

A

S

G

K

G

E

E

E

I

I

S

R

R

K

R

R

V

V

Q

E

D

E

V

V

L

A

E

K

R

I

V

L

G

D

L

I

P

H

G

H

Y

V

G

L

V

N

K

H

M

F

P

P

A

R

Q

E

L

L

S

S

G

G

Q

Q

K

Q

Q

Q

R

R

V

V

A

A

I

L

A

A

R

R

C

A

L

L

V

V

L

K

E

D

P

P

A

S

V

L

L

L

D

D

E
|
E

P

P

L

F

G

S

A

N

L

L

D

D

L

A

K

R

L

M

R

R

E

D

Q

S

M

A

K

R

V

A

E

L

L

V

K

K

T

E

L

V

Q

Q

A

S

E

R

V

L

G

G

T

V

T

T

F

L

V

L

Y

V

I

V

T

S

H

H

D

D

Q

P

S

A

E

D

A

I

L

F

V

A

M

I

S

A

D

D

H

R

V

V

A

G

V

V

M

L

N

V

A

K

G

G

R

K

F

L

E

V

Q

Q

V

V

D

G

T

K

P

P

R

E

N

D

L

L

Y

Y

R

D

R

N

P

P

A

V

S

S

A

I

F

Q

V

V

A

A

G

S

F

L

V

I

G

G

E

E

T

I

N

N

V

E

W

L

S

E

G

G

T

K

L

V

E

-

A

-

T

-

G

-

D

-

A

-

G

-

L

-

V

-

R

-

T

T

D

N

E

E

G

G

A

V

V

V

F

I

R

G

A

S

R

L

V

R

A

F

A

P

G

V

L

S

A

V

K

S

G

S

T

D

R

R

V

A

D

I

M

I

F

G

I

I

R

R

P

P

E

E

A

D

V

V

L

K

I

L

D

S

P

K

D

D

binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ V35), V18 (≠ F41), A20 (= A43), P39 (= P62), S40 (= S63), G41 (= G64), A42 (≠ C65), G43 (= G66), K44 (= K67), T45 (= T68), T46 (= T69), Q89 (= Q107), E166 (= E184)
binding magnesium ion: T45 (= T68), Q89 (= Q107)

1oxvA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
35% identity, 68% coverage: 30:315/418 of query aligns to 8:286/353 of 1oxvA

query
sites
1oxvA

S

N

V

V

T

S

R

K

L

V

V
x
F

K

K

R

K

F

-

E

G

K

K

F
x
V

T

V

A
|
A

V

L

N

D

D

N

V

V

S

N

F

I

E

N

V

I

E

E

Q

N

G

G

R

E

F

R

F

F

S

G

I

I

L

L

G

G

P
|
P

S
|
S

G
|
G

C
x
A

G
|
G

K
|
K

T
|
T

L

F

L

M