SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing ABIE51_RS17405 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9HTK8 Rubredoxin-2; Rdxs from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
74% identity, 86% coverage: 10:63/63 of query aligns to 1:54/55 of Q9HTK8

query
sites
Q9HTK8

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C6 (= C15) binding Fe cation
C9 (= C18) binding Fe cation
C39 (= C48) binding Fe cation
C42 (= C51) binding Fe cation

2v3bB Crystal structure of the electron transfer complex rubredoxin - rubredoxin reductase from pseudomonas aeruginosa. (see paper)
77% identity, 83% coverage: 10:61/63 of query aligns to 1:52/53 of 2v3bB

query
sites
2v3bB

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binding fe (iii) ion: C6 (= C15), C9 (= C18), C39 (= C48), C42 (= C51)

2kn9A Solution structure of zinc-substituted rubredoxin b (rv3250c) from mycobacterium tuberculosis. Seattle structural genomics center for infectious disease target mytud.01635.A (see paper)
56% identity, 94% coverage: 5:63/63 of query aligns to 20:78/81 of 2kn9A

query
sites
2kn9A

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binding zinc ion: C30 (= C15), C63 (= C48)

7a9aA Crystal structure of rubredoxin b (rv3250c) from mycobacterium tuberculosis (see paper)
59% identity, 89% coverage: 8:63/63 of query aligns to 2:57/60 of 7a9aA

query
sites
7a9aA

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binding fe (iii) ion: C9 (= C15), C12 (= C18), C42 (= C48), C45 (= C51)
binding zinc ion: D34 (≠ E40), D35 (= D41)

P00272 Rubredoxin-2; Rdxs; Two-iron rubredoxin from Ectopseudomonas oleovorans (Pseudomonas oleovorans) (see 2 papers)
54% identity, 90% coverage: 2:58/63 of query aligns to 111:167/173 of P00272

query
sites
P00272

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C124 (= C15) binding Fe cation
C127 (= C18) binding Fe cation
C157 (= C48) binding Fe cation
C160 (= C51) binding Fe cation

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
6 binding Fe cation
9 binding Fe cation
39 binding Fe cation
42 binding Fe cation

1s24A Rubredoxin domain ii from pseudomonas oleovorans (see paper)
57% identity, 84% coverage: 9:61/63 of query aligns to 1:53/56 of 1s24A

query
sites
1s24A

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binding cadmium ion: C7 (= C15), C10 (= C18), C40 (= C48), C43 (= C51)

2pvxB Nmr and x-ray analysis of structural additivity in metal binding site- swapped hybrids of rubredoxin (see paper)
56% identity, 83% coverage: 10:61/63 of query aligns to 1:52/54 of 2pvxB

query
sites
2pvxB

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binding zinc ion: C6 (= C15), C9 (= C18), C39 (= C48), C42 (= C51)

8f6tA Cryo-em structure of alkane 1-monooxygenase alkb-alkg complex from fontimonas thermophila (see paper)
55% identity, 79% coverage: 13:62/63 of query aligns to 377:427/428 of 8f6tA

query
sites
8f6tA

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binding fe (iii) ion: C379 (= C15), C382 (= C18), C412 (= C48), C415 (= C51)

Sites not aligning to the query:

binding dodecane: 122, 252
binding fe (iii) ion: 125, 129, 155, 159, 160, 259, 260, 299, 302, 303

6akxA The crystal structure of human chemokine receptor ccr5 in complex with compound 21 (see paper)
51% identity, 78% coverage: 11:59/63 of query aligns to 204:252/339 of 6akxA

query
sites
6akxA

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binding zinc ion: C208 (= C15), C211 (= C18), C241 (= C48), C244 (= C51)

Sites not aligning to the query:

binding N-[(1S)-3-{(3-exo)-3-[3-methyl-5-(propan-2-yl)-4H-1,2,4-triazol-4-yl]-8-azabicyclo[3.2.1]octan-8-yl}-1-(thiophen-2-yl)propyl]cyclopentanecarboxamide: 16, 65, 68, 87, 88, 91, 177, 281, 285, 313

4mbsA Crystal structure of the ccr5 chemokine receptor (see paper)
51% identity, 78% coverage: 11:59/63 of query aligns to 207:255/346 of 4mbsA

query
sites
4mbsA

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binding zinc ion: C211 (= C15), C214 (= C18), C244 (= C48), C247 (= C51)

Sites not aligning to the query:

binding 4,4-difluoro-N-[(1S)-3-{(3-exo)-3-[3-methyl-5-(propan-2-yl)-4H-1,2,4-triazol-4-yl]-8-azabicyclo[3.2.1]oct-8-yl}-1-phenylpropyl]cyclohexanecarboxamide: 19, 68, 71, 90, 91, 94, 164, 177, 281, 284, 288, 316

6akyA The crystal structure of human chemokine receptor ccr5 in complex with compound 34 (see paper)
51% identity, 78% coverage: 11:59/63 of query aligns to 206:254/340 of 6akyA

query
sites
6akyA

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binding zinc ion: C243 (= C48), C246 (= C51)

Sites not aligning to the query:

binding 4,4-difluoro-N-[(1S)-3-{(3-exo)-3-[3-methyl-5-(propan-2-yl)-4H-1,2,4-triazol-4-yl]-8-azabicyclo[3.2.1]octan-8-yl}-1-(thiophen-3-yl)propyl]cyclohexane-1-carboxamide: 18, 67, 70, 89, 90, 93, 175, 176, 179, 280, 283, 287, 315

P56263 Rubredoxin; Rd from Heliobacterium mobile (Heliobacillus mobilis) (see paper)
55% identity, 81% coverage: 11:61/63 of query aligns to 2:52/52 of P56263

query
sites
P56263

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Sites not aligning to the query:

1 modified: N-formylmethionine; partial

4xnwC The human p2y1 receptor in complex with mrs2500 (see paper)
51% identity, 78% coverage: 11:59/63 of query aligns to 212:260/350 of 4xnwC

query
sites
4xnwC

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binding zinc ion: C216 (= C15), C219 (= C18), C249 (= C48), C252 (= C51)

Sites not aligning to the query:

binding [(1R,2S,4S,5S)-4-[2-iodo-6-(methylamino)-9H-purin-9-yl]-2-(phosphonooxy)bicyclo[3.1.0]hex-1-yl]methyl dihydrogen phosphate: 5, 9, 158, 167, 168, 295, 299, 303, 315, 318

6gpsA Crystal structure of ccr2a in complex with mk-0812 (see paper)
51% identity, 78% coverage: 11:59/63 of query aligns to 197:245/330 of 6gpsA

query
sites
6gpsA

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Sites not aligning to the query:

binding [(3~{S},4~{S})-3-methoxyoxan-4-yl]-[(1~{R},3~{S})-3-propan-2-yl-3-[[3-(trifluoromethyl)-7,8-dihydro-5~{H}-1,6-naphthyridin-6-yl]carbonyl]cyclopentyl]azanium: 5, 9, 62, 81, 85, 154, 297, 298, 300, 304

6me9A Xfel crystal structure of human melatonin receptor mt2 in complex with ramelteon (see paper)
51% identity, 78% coverage: 11:59/63 of query aligns to 309:357/448 of 6me9A

query
sites
6me9A

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binding zinc ion: C313 (= C15), C316 (= C18), C346 (= C48), C349 (= C51)

Sites not aligning to the query:

binding N-{2-[(8S)-1,6,7,8-tetrahydro-2H-indeno[5,4-b]furan-8-yl]ethyl}propanamide: 193, 197, 201, 251, 268, 280, 387

5xpdA Sugar transporter of atsweet13 in inward-facing state with a substrate analog (see paper)
51% identity, 78% coverage: 11:59/63 of query aligns to 219:267/269 of 5xpdA

query
sites
5xpdA

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Sites not aligning to the query:

binding 2'-deoxycytidine-5'-monophosphate: 15, 18, 19, 70, 190, 191

6me8A Xfel crystal structure of human melatonin receptor mt2 (n86d) in complex with 2-phenylmelatonin (see paper)
51% identity, 78% coverage: 11:59/63 of query aligns to 309:357/449 of 6me8A

query
sites
6me8A

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binding zinc ion: C313 (= C15), C316 (= C18), C346 (= C48), C349 (= C51)

Sites not aligning to the query:

binding N-[2-(5-methoxy-2-phenyl-1H-indol-3-yl)ethyl]acetamide: 197, 200, 201, 251, 268, 387, 388, 414, 417, 418

6gpxA Crystal structure of ccr2a in complex with mk-0812 (see paper)
51% identity, 78% coverage: 11:59/63 of query aligns to 190:238/324 of 6gpxA

query
sites
6gpxA

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G

G

F

Y

I

I

Y

Y

D

N

E

P

A

E

L

D

G

G

L

D

P

P

E

D

E

N

G

G

I

V

A

N

P

P

G

G

T

T

R

D

W

F

E

K

D

D

V

I

P

P

D

D

T

D

W

W

T

V

C
|
C

P

P

D

L

C
|
C

G

G

V

V

T

G

K

K

D

D

D

Q

F

F

E

E

binding zinc ion: C194 (= C15), C227 (= C48), C230 (= C51)

Sites not aligning to the query:

binding [(3~{S},4~{S})-3-methoxyoxan-4-yl]-[(1~{R},3~{S})-3-propan-2-yl-3-[[3-(trifluoromethyl)-7,8-dihydro-5~{H}-1,6-naphthyridin-6-yl]carbonyl]cyclopentyl]azanium: 2, 6, 10, 14, 63, 82, 86, 147, 290, 291, 293, 294, 297

1zrpA Solution-state structure by nmr of zinc-substituted rubredoxin from the marine hyperthermophilic archaebacterium pyrococcus furiosus (see paper)
52% identity, 79% coverage: 12:61/63 of query aligns to 2:51/53 of 1zrpA

query
sites
1zrpA

T

K

W

W

M

V

C
|
C

V

K

V

I

C
|
C

G

G

F

Y

I

I

Y

Y

D

D

E

E

A

D

L

A

G

G

L

D

P

P

E

D

E

N

G

G

I

I

A

S

P

P

G

G

T

T

R

K

W

F

E

E

D

E

V

L

P

P

D

D

T

D

W

W

T

V

C
|
C

P

P

D

I

C
|
C

G

G

V

A

T

P

K

K

D

S

D

E

F

F

E

E

M

K

M

L

binding zinc ion: C5 (= C15), C8 (= C18), C38 (= C48), C41 (= C51)

9bktA Crystal structure of rubredoxin from pyrococcus furiosus reconstituted with feso4 solved by fe/s-sad
52% identity, 79% coverage: 12:61/63 of query aligns to 3:52/54 of 9bktA

query
sites
9bktA

T

K

W

W

M

V

C
|
C

V

K

V

I

C
|
C

G

G

F

Y

I

I

Y

Y

D

D

E

E

A

D

L

A

G

G

L

D

P

P

E

D

E

N

G

G

I

I

A

S

P

P

G

G

T

T

R

K

W

F

E

E

D

E

V

L

P

P

D

D

T

D

W

W

T

V

C
|
C

P

P

D

I

C
|
C

G

G

V

A

T

P

K

K

D

S

D

E

F

F

E

E

M

K

M

L

binding fe (ii) ion: C6 (= C15), C9 (= C18), C39 (= C48), C42 (= C51)

Query Sequence

>ABIE51_RS17405
MSETAATTTFRTWMCVVCGFIYDEALGLPEEGIAPGTRWEDVPDTWTCPDCGVTKDDFEM
MPL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory