SitesBLAST
Comparing AMB_RS14100 FitnessBrowser__Magneto:AMB_RS14100 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P04805 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Escherichia coli (strain K12) (see 4 papers)
44% identity, 98% coverage: 1:466/477 of query aligns to 1:461/471 of P04805
- C98 (= C98) mutation to S: 10-fold decrease in activity. Strong decrease in zinc content.
- C100 (= C100) mutation to S: Loss of activity. Strong decrease in zinc content.; mutation to Y: Does not prevent zinc binding. Reduces only 2-fold the binding affinity for tRNA(Glu), but reduces more than 10-fold the affinity for glutamate in the presence of tRNA(Glu).
- C125 (≠ W125) mutation to S: Loss of activity. Strong decrease in zinc content.
- H127 (≠ D127) mutation to Q: 10-fold decrease in activity. Strong decrease in zinc content.
- H129 (≠ P129) mutation to Q: No change in activity or in zinc content.
- H131 (≠ T131) mutation to Q: No change in activity or in zinc content.
- H132 (≠ D132) mutation to Q: No change in activity or in zinc content.
- C138 (≠ F139) mutation to S: No change in activity or in zinc content.
- S239 (= S240) modified: Phosphoserine; mutation to D: Does not aminoacylate tRNA(Glu), not phosphorylated by HipA.
8i9iA Glutamyl-tRNA synthetase from escherichia coli bound to glutamate and zinc
44% identity, 98% coverage: 1:466/477 of query aligns to 1:461/468 of 8i9iA
4g6zA Crystal structure of a glutamyl-tRNA synthetase glurs from burkholderia thailandensis bound to l-glutamate (see paper)
48% identity, 77% coverage: 3:370/477 of query aligns to 3:355/380 of 4g6zA
1g59A Glutamyl-tRNA synthetase complexed with tRNA(glu). (see paper)
41% identity, 97% coverage: 3:467/477 of query aligns to 2:468/468 of 1g59A
- binding : D44 (= D45), R45 (= R46), A46 (= A47), R47 (= R48), P109 (≠ Q102), V145 (= V140), R163 (≠ Q158), V166 (= V161), E172 (≠ Q167), V177 (= V172), K180 (≠ R175), S181 (≠ A176), D182 (= D177), E207 (≠ D202), E208 (≠ D203), R237 (≠ H232), K241 (≠ G236), T242 (≠ A237), K243 (= K238), M273 (≠ L268), G274 (= G269), E282 (= E276), S299 (≠ G293), L300 (≠ R294), P303 (≠ S297), V304 (≠ R298), K309 (= K303), W312 (≠ N306), R319 (= R313), P357 (≠ E353), R358 (= R354), R417 (= R416), K426 (= K425), L427 (= L426), Q432 (= Q431), R435 (= R434), L442 (≠ S441), E443 (≠ V442), T444 (≠ S443), P445 (= P444), G446 (≠ P445), L447 (≠ I446), F448 (= F447)
2cv2A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu) and an enzyme inhibitor, glu-ams (see paper)
41% identity, 97% coverage: 3:467/477 of query aligns to 2:468/468 of 2cv2A
- active site: K246 (= K241)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R5 (= R6), A7 (= A8), S9 (= S10), G17 (= G18), I21 (≠ T22), E41 (= E42), Y187 (= Y182), R205 (= R200), A206 (≠ G201), E208 (≠ D203), W209 (≠ H204), L235 (= L230), L236 (≠ I231)
- binding : S9 (= S10), T43 (= T44), D44 (= D45), R47 (= R48), V145 (= V140), R163 (≠ Q158), Y168 (≠ V163), E172 (≠ Q167), V177 (= V172), K180 (≠ R175), S181 (≠ A176), Y187 (= Y182), E207 (≠ D202), E208 (≠ D203), W209 (≠ H204), V211 (≠ T206), R237 (≠ H232), K241 (≠ G236), L272 (≠ R267), M273 (≠ L268), G274 (= G269), E282 (= E276), S299 (≠ G293), P303 (≠ S297), V304 (≠ R298), K309 (= K303), W312 (≠ N306), R319 (= R313), P357 (≠ E353), R358 (= R354), R417 (= R416), Q432 (= Q431), R435 (= R434), L442 (≠ S441), E443 (≠ V442), T444 (≠ S443), G446 (≠ P445), L447 (≠ I446), F448 (= F447)
2cv1A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu), atp, and an analog of l-glutamate: a quaternary complex
41% identity, 97% coverage: 3:467/477 of query aligns to 2:468/468 of 2cv1A
- active site: K246 (= K241)
- binding adenosine-5'-triphosphate: P8 (= P9), S9 (= S10), G17 (= G18), T18 (≠ G19), I21 (≠ T22), R47 (= R48), A206 (≠ G201), W209 (≠ H204), L235 (= L230), L236 (≠ I231)
- binding (4s)-4-amino-5-hydroxypentanoic acid: R5 (= R6), A7 (= A8), E41 (= E42), Y187 (= Y182), R205 (= R200), W209 (≠ H204)
- binding : S9 (= S10), E41 (= E42), T43 (= T44), D44 (= D45), R47 (= R48), V145 (= V140), R163 (≠ Q158), V166 (= V161), E172 (≠ Q167), V177 (= V172), K180 (≠ R175), S181 (≠ A176), Y187 (= Y182), E207 (≠ D202), E208 (≠ D203), W209 (≠ H204), V211 (≠ T206), R237 (≠ H232), K241 (≠ G236), K243 (= K238), M273 (≠ L268), G274 (= G269), S276 (= S271), E282 (= E276), S299 (≠ G293), P303 (≠ S297), V304 (≠ R298), K309 (= K303), W312 (≠ N306), R319 (= R313), P357 (≠ E353), R358 (= R354), R417 (= R416), L427 (= L426), Q432 (= Q431), R435 (= R434), L442 (≠ S441), E443 (≠ V442), T444 (≠ S443), G446 (≠ P445), L447 (≠ I446), F448 (= F447)
2cuzA Glutamyl-tRNA synthetase from thermus thermophilus in complex with l- glutamate (see paper)
41% identity, 97% coverage: 3:467/477 of query aligns to 2:468/468 of 2cuzA
1n78A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with tRNA(glu) and glutamol-amp. (see paper)
41% identity, 97% coverage: 3:467/477 of query aligns to 2:468/468 of 1n78A
- active site: K246 (= K241)
- binding glutamol-amp: R5 (= R6), A7 (= A8), P8 (= P9), S9 (= S10), G17 (= G18), T18 (≠ G19), I21 (≠ T22), E41 (= E42), Y187 (= Y182), N191 (≠ V186), R205 (= R200), A206 (≠ G201), E208 (≠ D203), W209 (≠ H204), L235 (= L230), L236 (≠ I231)
- binding : S9 (= S10), T43 (= T44), D44 (= D45), R47 (= R48), V145 (= V140), R163 (≠ Q158), V166 (= V161), Y168 (≠ V163), E172 (≠ Q167), V177 (= V172), K180 (≠ R175), S181 (≠ A176), Y187 (= Y182), E207 (≠ D202), E208 (≠ D203), W209 (≠ H204), L210 (= L205), V211 (≠ T206), R237 (≠ H232), K241 (≠ G236), M273 (≠ L268), G274 (= G269), E282 (= E276), R297 (≠ S291), P303 (≠ S297), V304 (≠ R298), K309 (= K303), W312 (≠ N306), R319 (= R313), P357 (≠ E353), R358 (= R354), R417 (= R416), L427 (= L426), Q432 (= Q431), R435 (= R434), L442 (≠ S441), E443 (≠ V442), T444 (≠ S443), G446 (≠ P445), L447 (≠ I446), F448 (= F447)
1j09A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with atp and glu (see paper)
41% identity, 97% coverage: 3:467/477 of query aligns to 2:468/468 of 1j09A
- active site: K246 (= K241)
- binding adenosine-5'-triphosphate: H15 (= H16), E208 (≠ D203), L235 (= L230), L236 (≠ I231), K243 (= K238), I244 (≠ L239), S245 (= S240), K246 (= K241), R247 (= R242)
- binding glutamic acid: R5 (= R6), A7 (= A8), S9 (= S10), E41 (= E42), Y187 (= Y182), N191 (≠ V186), R205 (= R200), W209 (≠ H204)
P27000 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
41% identity, 97% coverage: 3:467/477 of query aligns to 2:468/468 of P27000
- R358 (= R354) mutation to Q: Reduces affinity for tRNA and abolishes the ability to discriminate between tRNA(Glu) and tRNA(Gln).
4griB Crystal structure of a glutamyl-tRNA synthetase glurs from borrelia burgdorferi bound to glutamic acid and zinc (see paper)
35% identity, 97% coverage: 5:465/477 of query aligns to 4:478/485 of 4griB
- active site: S9 (= S10), K253 (= K241)
- binding glutamic acid: R5 (= R6), A7 (= A8), S9 (= S10), E41 (= E42), Y194 (= Y182), R212 (= R200), W216 (≠ H204)
- binding zinc ion: C105 (= C98), C107 (= C100), Y128 (= Y121), C132 (≠ W125)
3al0C Crystal structure of the glutamine transamidosome from thermotoga maritima in the glutamylation state. (see paper)
36% identity, 97% coverage: 3:466/477 of query aligns to 103:559/564 of 3al0C
- active site: S110 (= S10), K335 (= K241)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R106 (= R6), A108 (= A8), P109 (= P9), G118 (= G18), T122 (= T22), E142 (= E42), Y276 (= Y182), R294 (= R200), G295 (= G201), D297 (= D203), H298 (= H204), L324 (= L230), I325 (= I231), L333 (= L239)
- binding : T144 (= T44), D145 (= D45), R148 (= R48), Y208 (≠ Q102), P213 (≠ E111), K252 (≠ Q158), M255 (≠ V161), I266 (≠ V172), K269 (≠ R175), S270 (≠ A176), Y276 (= Y182), D297 (= D203), H298 (= H204), L299 (= L205), S300 (≠ T206), N301 (= N207), K304 (≠ R210), R330 (≠ G236), P332 (≠ K238), G363 (= G269), W364 (= W270), R365 (≠ S271), E370 (= E276), S387 (≠ G293), K389 (≠ S295), V391 (≠ S297), I392 (≠ R298), K397 (= K303), W400 (≠ N306), R407 (= R313), E446 (= E353), K447 (≠ R354), Q453 (≠ E360), I457 (≠ S364), R509 (= R416), K520 (≠ G427), Q524 (= Q431), R527 (= R434), V535 (= V442), T536 (≠ S443), G538 (≠ P445), L539 (≠ I446)
8vc5A Crystal structure of glutamyl-tRNA synthetase glurs from pseudomonas aeruginosa (zinc bound)
36% identity, 97% coverage: 2:466/477 of query aligns to 3:466/488 of 8vc5A
Q8DLI5 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1) (see paper)
39% identity, 95% coverage: 1:453/477 of query aligns to 1:464/485 of Q8DLI5
- R6 (= R6) binding L-glutamate
- Y192 (= Y182) binding L-glutamate
2cfoA Non-discriminating glutamyl-tRNA synthetase from thermosynechococcus elongatus in complex with glu (see paper)
38% identity, 95% coverage: 2:453/477 of query aligns to 1:463/484 of 2cfoA
6brlA Crystal structure of a glutamate tRNA ligase from elizabethkingia meningosepticum ccug26117 in complex with its amino acid (see paper)
35% identity, 97% coverage: 3:466/477 of query aligns to 3:498/502 of 6brlA
4a91A Crystal structure of the glutamyl-queuosine trnaasp synthetase from e. Coli complexed with l-glutamate (see paper)
36% identity, 51% coverage: 4:246/477 of query aligns to 5:234/290 of 4a91A
- active site: S11 (= S10), K229 (= K241)
- binding glutamic acid: R7 (= R6), A9 (= A8), S11 (= S10), E43 (= E42), Y170 (= Y182), R188 (= R200), L192 (≠ H204)
- binding zinc ion: C99 (= C98), C101 (= C100), Y113 (≠ W125), C117 (≠ D127)
P27305 Glutamyl-Q tRNA(Asp) synthetase; Glu-Q-RSs; EC 6.1.1.- from Escherichia coli (strain K12) (see paper)
36% identity, 51% coverage: 4:245/477 of query aligns to 17:245/308 of P27305
- E55 (= E42) binding L-glutamate
- Y182 (= Y182) binding L-glutamate
- R200 (= R200) binding L-glutamate
3aiiA Archaeal non-discriminating glutamyl-tRNA synthetase from methanothermobacter thermautotrophicus (see paper)
33% identity, 56% coverage: 3:269/477 of query aligns to 11:278/455 of 3aiiA
4h3sA The structure of glutaminyl-tRNA synthetase from saccharomyces cerevisiae (see paper)
37% identity, 22% coverage: 3:105/477 of query aligns to 38:139/585 of 4h3sA
Sites not aligning to the query:
Query Sequence
>AMB_RS14100 FitnessBrowser__Magneto:AMB_RS14100
MTVVTRFAPSPTGFLHIGGGRTALFNWLFARHHGGKFLLRIEDTDRARSTDAAVEAIFDG
IKWLGLDWDGEAVMQFARACRHAEVARQLLDEGKAYRCYCTQDELTAIREEQKAKGQPMR
YPGIWRDRPATDAPEGAPFVVRLKAPAEGETIIADLVQGDVRVANDQLDDMVLLRADGTP
TYMLSVVVDDHDMGITHVIRGDDHLTNAFRQYQLYKACGWEVPTFAHIPLIHGPDGAKLS
KRHGALGVDAYRDMGFLPEAMRNYLLRLGWSHGDDEIISTEQAVEWFTLDSVGRSPSRFD
FVKLTNLNGHYMRGADDGRLTEVLVPLLEAKTGKALSGESVARLRTGMTGLKERAKTMVE
LADSALFYVAERPLALDEKAAKTMADETATADLAAYRTEVKELGAWTRDTLEDAARRLAE
ARGQKLGKIAQPLRAALAGSSVSPPIFEVMEVLGREESLGRIEDALGGTRPTTSTAA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory