SitesBLAST

Q8U2I1 6-oxopurine nucleoside phosphorylase; Purine nucleoside phosphorylase; PNP; PfPNP; EC 2.4.2.1 from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) (see 2 papers)
42% identity, 94% coverage: 1:230/245 of query aligns to 1:232/265 of Q8U2I1

query
sites
Q8U2I1

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M1 (= M1) modified: Initiator methionine, Removed
C136 (≠ S134) modified: Disulfide link with 202
C162 (= C160) modified: Disulfide link with 190
E169 (= E167) mutation to S: Decreases catalytic activity for insosine and allows the enzyme to phosphorolytically cleave MTA with a catalytic efficiency about 4-fold higher than for inosine; when associated with D-211, D-213 and A-223.
C190 (≠ M188) modified: Disulfide link with 162
C202 (≠ D200) modified: Disulfide link with 136
N211 (= N209) mutation to D: Decreases catalytic activity for insosine and allows the enzyme to phosphorolytically cleave MTA with a catalytic efficiency about 4-fold higher than for inosine; when associated with S-169, D-213 and A-223.
A213 (= A211) mutation to D: Decreases catalytic activity for insosine and allows the enzyme to phosphorolytically cleave MTA with a catalytic efficiency about 4-fold higher than for inosine; when associated with S-169, D-211 and A-223.
H223 (≠ M221) mutation to A: Decreases catalytic activity for insosine and allows the enzyme to phosphorolytically cleave MTA with a catalytic efficiency about 4-fold higher than for inosine; when associated with S-169, D-211 and D-213.

Sites not aligning to the query:

254 modified: Disulfide link with 256; C→S: Fully active, but reduces thermodynamic and kinetic stability of the enzyme; when associated with S-256.
256 modified: Disulfide link with 254; C→S: Fully active, but reduces thermodynamic and kinetic stability of the enzyme; when associated with S-254.

9jd2A Crystal structure of 5'-deoxy-5'-methylthioadenosine phosphorylase from aeropyrum pernix complex with 5'-deoxy-5'-methylthioadenosine 353k
40% identity, 97% coverage: 6:243/245 of query aligns to 14:250/273 of 9jd2A

query
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9jd2A

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binding 5'-deoxy-5'-methylthioadenosine: V95 (= V87), G96 (= G88), F173 (≠ L166), I191 (≠ V183), M193 (= M185), D217 (≠ N209), V231 (≠ I224)

1wtaA Crystal structure of 5'-deoxy-5'-methylthioadenosine from aeropyrum pernix (r32 form)
40% identity, 97% coverage: 6:243/245 of query aligns to 14:250/273 of 1wtaA

query
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1wtaA

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active site: D217 (≠ N209), D219 (≠ A211)
binding adenine: V95 (= V87), G96 (= G88), F173 (≠ L166), I191 (≠ V183), D217 (≠ N209), D219 (≠ A211)

Q8U4Q8 S-methyl-5'-thioadenosine phosphorylase; 5'-methylthioadenosine phosphorylase; MTA phosphorylase; MTAP; PfMTAP; EC 2.4.2.28 from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) (see paper)
36% identity, 99% coverage: 1:243/245 of query aligns to 1:237/257 of Q8U4Q8

query
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Q8U4Q8

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C130 (≠ S134) modified: Disulfide link with 195
C195 (≠ D200) modified: Disulfide link with 130

Sites not aligning to the query:

246 modified: Disulfide link with 248
248 modified: Disulfide link with 246

3t94A Crystal structure of 5'-deoxy-5'-methylthioadenosine phosphorylase (mtap) ii complexed with 5'-deoxy-5'-methylthioadenosine and sulfate (see paper)
37% identity, 96% coverage: 6:239/245 of query aligns to 12:243/270 of 3t94A

query
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3t94A

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V

I

-

T

T

M
x
A

A

E

E

E

I
x
V

E

T

Q

R

A

V

L

M

H

A

E

E

G

N

M

T

G

E

K

K

V

A

K

K

S

K

T

L

L

L

active site: S16 (≠ T10), P39 (= P33), H63 (= H57), I65 (≠ F59), P66 (= P60), A92 (= A86), M190 (= M185), T191 (= T186), D214 (≠ N209), D216 (≠ A211), A225 (≠ M221)
binding 5'-deoxy-5'-methylthioadenosine: G94 (= G88), F170 (≠ L166), I188 (≠ V183), M190 (= M185), D214 (≠ N209), A225 (≠ M221), V228 (≠ I224)

Q97W94 S-methyl-5'-thioadenosine phosphorylase; 5'-methylthioadenosine phosphorylase; MTA phosphorylase; MTAP; MTAPII; EC 2.4.2.28 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see 2 papers)
37% identity, 96% coverage: 6:239/245 of query aligns to 12:243/270 of Q97W94

query
sites
Q97W94

I

I

G

G

T

S

G

G

L

L

T

Y

Q

D

L

P

E

G

G

I

L

F

S

S

I

E

R

S

Q

K

S

E

L

I

A

K

L

V

D

Y

T

T

P

P

Y

Y

G

G

A

Q

P

P

S

S

A

D

E

F

I

I

Q

T

V

I

G

G

E

K

Y

I

A

G

G

N

R

K

E

S

V

V

L

A

F

F

L

L

A

P

R

R

H

H

G

G

H

R

P

G

H

H

R

R

F

I

P

P

H

H

Q

K

V

I

N

N

Y

Y

R

R

A

A

N

N

L

I

W

W

A

A

L

L

K

K

Q

E

A

L

G

G

A

V

Q

R

A

W

I

V

L

I

A

S

V

V

N

S

A

A

V

V

G

G

G

S

I

L

H

R

A

M

A

D

M

Y

G

K

T

L

G

G

H

D

F

F

C

V

V

I

P

P

H

D

Q

Q

L

F

I

I

D

D

Y

M

T

T

S

K

G

N

R

R

Q

E

H

Y

T

S

Y

F

F

F

A

-

D

-

D

D

L

G

E

P

Q

V

V

V

T

A

H

H

I

V

D

S

F

M

S

A

Y

D

P

P

Y

F

S
x
C

E

N

P

S

L

L

R

R

E

K

Q

L

L

A

I

I

A

E

A

T

L

A

A

K

A

E

E

L

G

N

C

I

A

K

Y

T

S

H

S

E

H

S

G

G

V

T

Y

Y

A

I

C

C

T

I

Q

E

G

G

P

P

R

R

L

F

E

S

T

T

V

R

A

A

E

E

I

-

A

S

R

R

L

T

E

W

R

R

D

E

-

V

-

Y

G

K

C

A

D

D

I

I

V

I

G

G

M

M

T

T

G

L

M

V

P

P

E

E

A

V

A

N

L

L

A

A

R
x
C

E

E

L

A

E

Q

L

M

D
x
C

Y

Y

A

A

C

T

L

I

A

A

L

M

V

V

V

T

N

D

P

Y

A

D

A

V

G

F

K

A

S

E

T

I

A

P

V

V

I

-

T

T

M

A

A

E

E

E

I

V

E

T

Q

R

A

V

L

M

H

A

E

E

G

N

M

T

G

E

K

K

V

A

K

K

S

K

T

L

L

L

C138 (≠ S134) modified: Disulfide link with 205
C200 (≠ R195) modified: Disulfide link with 262
C205 (≠ D200) modified: Disulfide link with 138

Sites not aligning to the query:

259 modified: Disulfide link with 261; C→S: Reduces thermostability of the enzyme; when associated with S-261.
261 modified: Disulfide link with 259; C→S: Reduces thermostability of the enzyme; when associated with S-259.
262 modified: Disulfide link with 200; C→S: Reduces thermostability of the enzyme.

4gljA Crystal structure of methylthioadenosine phosphorylase in complex with rhodamine b (see paper)
41% identity, 84% coverage: 5:209/245 of query aligns to 7:212/287 of 4gljA

query
sites
4gljA

A

A

I

I

G

G

T
x
S

G

G

L

L

T
x
Y

Q

Q

L

M

E

Q

G

A

L

L

S

T

I

N

R

K

Q

R

S

S

L

V

A

K

L

I

D

E

T

T

P

P

Y

Y

G

G

A

E

P

P

S

S

A

D

E

D

I

I

Q

V

V

L

G

G

E

E

Y

L

A

N

G

G

R

V

E

T

V

V

L

A

F

F

L

L

A

T

R

R

H
|
H

G

G

H

Q

P

G

H

H

R

R

F

L

P

T

P

P

H

S

Q

E

V

V

N

P

Y

Y

R

R

A

A

N

N

L

I

W

Y

A

A

L

L

K

K

Q

T

A

L

G

G

A

V

Q

R

A

Y

I

I

L

V

A

S

V

V

N

S

A
|
A

V

V

G
|
G

G

S

I

L

H

Q

A

E

A

T

M

L

G

K

T

P

G

L

H

D

F

M

C

V

V

I

P

P

H

D

Q

Q

L

M

I

I

D

D

Y

M

T

T

S

K

G

Q

R

R

Q

V

H

S

T

T

Y

F

F

F

A

G

D

D

D

G

L

-

E

-

Q

A

V

V

T

A

H

H

I

V

D

S

F

M

S

A

Y

D

P

P

Y

L

S

C

E

P

P

E

L

V

R

A

E

D

Q

I

L

L

I

I

A

R

A

A

L

Y

-

D

-

N

-

A

-

D

A

I

A

A

E

D

G

G

C

Q

A

C

Y

H

S

A

S

K

H

-

G

A

V

T

Y

Y

A

V

C

C

T

I

Q

E

G

G

P

P

R

Q

L
x
F

E

S

T

T

V

R

A

A

E

E

I

S

A

H

R

W

L

Y

E

R

R

Q

D

M

G

Q

C

A

D

D

I

I

V
x
I

G
|
G

M
|
M

T

T

G

N

M

M

P

P

E

E

A

A

A

K

L

L

A

A

R

R

E

E

L

A

E

S

L

I

D

A

Y

Y

A

A

C

T

L

L

A

A

L

L

V

V

V

T

N
x
D

active site: D212 (≠ N209)
binding N-[9-(2-carboxyphenyl)-6-(diethylamino)-3H-xanthen-3-ylidene]-N-ethylethanaminium: S12 (≠ T10), Y15 (≠ T13), H55 (= H53), A88 (= A86), G90 (= G88), F169 (≠ L166), F169 (≠ L166), I186 (≠ V183), G187 (= G184), M188 (= M185), D212 (≠ N209)

Sites not aligning to the query:

active site: 214
binding N-[9-(2-carboxyphenyl)-6-(diethylamino)-3H-xanthen-3-ylidene]-N-ethylethanaminium: 213, 214, 219, 228, 229, 232

4l5aA Methylthioadenosine phosphorylase from schistosoma mansoni in complex with tubercidin (see paper)
35% identity, 89% coverage: 6:223/245 of query aligns to 6:239/282 of 4l5aA

query
sites
4l5aA

I

I

G

G

T

S

G

G

L

F

T

D

Q

L

L

F

E

K

G

K

L

V

S

G

I

V

R

R

Q

Q

S

-

L

-

A

-

L

V

D

T

T

T

P

P

Y

F

G

G

A
x
K

P

P

S

S

A

D

E

T

I

L

Q

V

V

E

G

G

E

F

Y

V

A

G

G

D

R

V

E

A

V

C

L

V

F

V

L

L

A

P

R

R

H

H

G

G

H

K

P

G

H
|
H

R

L

F

I

P

P

H

S

Q

E

V

V

N

N

Y

Y

R

R

A

A

N

N

L

V

W

W

A

A

L

L

K

K

Q

D

A

L

G

G

A

C

Q

T

A

H

I

I

L

L

A

A

V

T

N

N

A
|
A

V
x
C

G
|
G

G

S

I

L

H

Q

A

E

A

D

M

L

G

V

T

P

G

G

H

D

F

F

C

V

V

V

P

L

H

N

Q

Q

L

F

I

M

D

D

Y

K

T

T

S

W

G

G

R

R

Q

E

H

N

T

T

Y

F

F

Y

A

G

-

S

-

K

-

P

D

D

D

S

L

L

E

K

Q

G

V

V

T

L

H

H

I

M

D

P

F

M

S

A

Y

E

P

P

Y

F

S

C

E

E

P

R

L

T

R

R

E

Q

Q

I

L

L

I

I

A

Q

A

A

-

A

-

R

-

N

-

K

-

S

-

I

-

N

-

V

-

Y

-

D

-

K

-

T

L

M

A

D

A

K

E

S

G

A

C

C

A

I

Y

H

S

P

-

C

-

V

-

H

S

A

H

E

G

G

V

S

Y

A

A

V

C

T

T

I

Q

N

G

G

P

P

R

R

L
x
F

E

S

T

T

V

R

A

C

E

E

I

S

A

F

R

I

L

H

E

K

R

A

D

M

G

G

C

L

D

D

I

I

V

V

G
x
N

M
|
M

T

T

G

L

M

V

P

P

E

E

A

V

A

S

L

L

A

A

R

R

E

E

L

A

E

G

L

L

D

S

Y

Y

A

A

C

S

L

I

A

A

L

I

V

V

V

T

N
x
D

P

F

A
x
D

A

C

G

W

K

K

S

S

T

V

A

C

V

V

I

D

T

M

M
x
V

A

L

E

E

active site: K29 (≠ A32), H54 (= H57), D225 (≠ N209), D227 (≠ A211)
binding '2-(4-amino-pyrrolo[2,3-d]pyrimidin-7-yl)-5-hydroxymethyl-tetrahydro-furan-3,4-diol: A83 (= A86), C84 (≠ V87), G85 (= G88), F182 (≠ L166), N200 (≠ G184), M201 (= M185), V237 (≠ M221)

1sd2A Structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase complexed with 5'-methylthiotubercidin (see paper)
33% identity, 96% coverage: 6:239/245 of query aligns to 6:232/262 of 1sd2A

query
sites
1sd2A

I

I

G

G

T
|
T

G

G

L

L

T

D

Q

D

L

P

E

E

G

I

L

L

S

E

I

G

R

R

Q

T

S

E

L

K

A

Y

L

V

D

D

T

T

P

P

Y

F

G

G

A

K

P
|
P

S

S

A

D

E

A

I

L

Q

I

V

L

G

G

E

K

Y

I

A

K

G

N

R

V

E

D

V

C

L

V

F

L

L

L

A

A

R

R

H

H

G

G

H

R

P

Q

H
|
H

R

T

F
x
I

P
x
M

P

P

H

S

Q

K

V

V

N

N

Y

Y

R

Q

A

A

N

N

L

I

W

W

A

A

L

L

K

K

Q

E

A

E

G

G

A

C

Q

T

A

H

I

V

L

I

A

V

V

T

N

T

A
|
A

V
x
C

G
|
G

G

S

I

L

H

R

A

E

M

I

G

Q

T

P

G

G

H

D

F

I

C

V

V

I

P

I

H

D

Q

Q

L

F

I

I

D

D

Y

R

T

T

S

T

G

M

R

R

Q

P

H

Q

T

S

Y

F

F

Y

A

D

D

G

L

-

E

-

Q

-

V

V

T

C

H

H

I

I

D

P

F

M

S

A

Y

E

P

P

Y

F

S

C

E

P

P

K

L

T

R

R

E

E

Q

V

L

L

I

I

A

E

A

T

L

A

A

K

E

L

G

G

C

L

A

R

Y

C

S

H

S

S

H

K

G

G

V

T

Y

M

A

V

C

T

T

I

Q

E

G

G

P

P

R

R

L
x
F

E

S

T

S

V

R

A

A

E

E

I

S

A

F

R

M

L

F

E

R

R

T

D

W

G

G

C

A

D

D

I

V

V
x
I

G
x
N

M
|
M

T
|
T

G

T

M

V

P

P

E

E

A

V

L

L

A

A

R

K

E

E

L

A

E

G

L

I

D

C

Y

Y

A

A

C

S

L

I

A

A

L

M

V

-

N

-

P

-

A

A

A

T

G
x
D

K

Y

S
x
D

T

C

A

W

V

A

I

V

T

S

M
x
V

A

D

E

R

I
x
V

E

L

Q

K

A

T

L

L

H

K

E

E

G

N

M

A

G

N

K

K

V

A

K

K

S

S

T

L

L

L

active site: T10 (= T10), P33 (= P33), H57 (= H57), I59 (≠ F59), M60 (≠ P60), A86 (= A86), M182 (= M185), T183 (= T186), D206 (≠ G213), D208 (≠ S215), V214 (≠ M221)
binding 2-(4-amino-pyrrolo[2,3-d]pyrimidin-7-yl)-5-methylsulfanylmethyl-tetrahydro-furan-3,4-diol: C87 (≠ V87), G88 (= G88), F163 (≠ L166), I180 (≠ V183), N181 (≠ G184), M182 (= M185), D206 (≠ G213), V217 (≠ I224)

6dz0A Crystal structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase in complex with (3r,4s)-1-((4-amino-5h-pyrrolo[3,2- d]pyrimidin-7-yl)methyl)-4-((pent-4-yn-1-ylthio)methyl)pyrrolidin-3- ol (see paper)
32% identity, 96% coverage: 6:239/245 of query aligns to 8:245/274 of 6dz0A

query
sites
6dz0A

I

I

G

G

G
|
G

T
|
T

G

G

L

L

T

D

Q

D

L

P

E

E

G

I

L

L

S

E

I

G

R

R

Q

T

S

E

L

K

A

Y

L

V

D

D

T

T

P

P

Y

F

G

G

A

K

P
|
P

S

S

A

D

E

A

I

L

Q

I

V

L

G

G

E

K

Y

I

A

K

G

N

R

V

E

D

V

C

L

V

F

L

L

L

A

A

R
|
R

H
|
H

G

G

H

R

P

Q

H
|
H

R

T

F
x
I

P
x
M

P

P

H

S

Q

K

V

V

N

N

Y

Y

R

Q

A

A

N

N

L

I

W

W

A

A

L

L

K

K

Q

E

A

E

G

G

A

C

Q

T

A

H

I

V

L

I

A

V

V

T

N
x
T

A
|
A

V
x
C

G
|
G

G

S

I

L

H

R

A

E

M

I

G

Q

T

P

G

G

H

D

F

I

C

V

V

I

P

I

H

D

Q

Q

L

F

I

I

D

D

Y

R

T

T

S

T

G

M

R

R

Q

P

H

Q

T

S

Y

F

F

Y

A

D

D

G

D

S

-

H

-

S

-

C

L

A

E

R

Q

G

V

V

T

C

H

H

I

I

D

P

F

M

S

A

Y

E

P

P

Y

F

S

C

E

P

P

K

L

T

R

R

E

E

Q

V

L

L

I

I

A

E

A

T

L

A

A

K

E

L

G

G

C

L

A

R

Y

C

S

H

S

S

H

K

G

G

V

T

Y

M

A

V

C

T

T

I

Q

E

G

G

P

P

R

R

L
x
F

E

S

T

S

V

R

A

A

E

E

I

S

A

F

R

M

L

F

E

R

R

T

D

W

G

G

C

A

D

D

I

V

V
x
I

G
x
N

M
|
M

T
|
T

G

T

M

V

P

P

E

E

A

V

L

L

A

A

R

K

E

E

L

A

E

G

L

I

D

C

Y

Y

A

A

C

S

L

I

A

A

L

M

V

A

V
x
T

N
x
D

P

Y

A
x
D

A

C

G

W

K

K

S

E

-

H

T

E

A

E

V

A

I

V

T

S

M
x
V

A

D

E

R

I
x
V

E

L

Q

K

A

T

L

L

H

K

E

E

G

N

M

A

G

N

K

K

V

A

K

K

S

S

T

L

L

L

active site: T12 (= T10), P35 (= P33), H59 (= H57), I61 (≠ F59), M62 (≠ P60), A88 (= A86), M190 (= M185), T191 (= T186), D214 (≠ N209), D216 (≠ A211), V227 (≠ M221)
binding (3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-{[(pent-4-yn-1-yl)sulfanyl]methyl}pyrrolidin-3-ol: T12 (= T10), H59 (= H57), A88 (= A86), C89 (≠ V87), G90 (= G88), F171 (≠ L166), I188 (≠ V183), N189 (≠ G184), M190 (= M185), T213 (≠ V208), D214 (≠ N209), D216 (≠ A211), V227 (≠ M221), V230 (≠ I224)
binding phosphate ion: G11 (= G9), T12 (= T10), R54 (= R52), H55 (= H53), T87 (≠ N85), T191 (= T186)

5eubA Crystal structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase in complex with 2-amino-mta and sulfate
32% identity, 96% coverage: 6:239/245 of query aligns to 5:242/272 of 5eubA

query
sites
5eubA

I

I

G

G

T
|
T

G

G

L

L

T

D

Q

D

L

P

E

E

G

I

L

L

S

E

I

G

R

R

Q

T

S

E

L

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A

Y

L

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D

D

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T

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P

Y

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G

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P

S

S

A

D

E

A

I

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I

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G

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Y

I

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L

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L

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A

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H
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H

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x
I

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x
M

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P

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Q

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V

N

N

Y

Y

R

Q

A

A

N

N

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I

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W

A

A

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L

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K

Q

E

A

E

G

G

A

C

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T

A

H

I

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L

I

A

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N

T

A
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A

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x
C

G
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G

G

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A

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M

I

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G

G

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F

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P

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Q

L

F

I

I

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D

Y

R

T

T

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M

R

R

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P

H

Q

T

S

Y

F

F

Y

A

D

D

G

D

S

-

H

-

S

-

C

L

A

E

R

Q

G

V

V

T

C

H

H

I

I

D

P

F

M

S

A

Y

E

P

P

Y

F

S

C

E

P

P

K

L

T

R

R

E

E

Q

V

L

L

I

I

A

E

A

T

L

A

A

K

E

L

G

G

C

L

A

R

Y

C

S

H

S

S

H

K

G

G

V

T

Y

M

A

V

C

T

T

I

Q

E

G

G

P

P

R

R

L
x
F

E

S

T

S

V

R

A

A

E

E

I

S

A

F

R

M

L

F

E

R

R

T

D

W

G

G

C

A

D

D

I

V

V
x
I

G
x
N

M
|
M

T
|
T

G

T

M

V

P

P

E

E

A

V

L

L

A

A

R

K

E

E

L

A

E

G

L

I

D

C

Y

Y

A

A

C

S

L

I

A

A

L

M

V

A

V
x
T

N
x
D

P

Y

A
x
D

A

C

G

W

K

K

S

E

-

H

T

E

A

E

V

A

I

V

T

S

M
x
V

A

D

E

R

I
x
V

E

L

Q

K

A

T

L

L

H

K

E

E

G

N

M

A

G

N

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K

V

A

K

K

S

S

T

L

L

L

active site: T9 (= T10), P32 (= P33), H56 (= H57), I58 (≠ F59), M59 (≠ P60), A85 (= A86), M187 (= M185), T188 (= T186), D211 (≠ N209), D213 (≠ A211), V224 (≠ M221)
binding (2~{R},3~{R},4~{S},5~{S})-2-[2,6-bis(azanyl)purin-9-yl]-5-(methylsulfanylmethyl)oxolane-3,4-diol: A85 (= A86), C86 (≠ V87), G87 (= G88), F168 (≠ L166), I185 (≠ V183), N186 (≠ G184), T210 (≠ V208), D211 (≠ N209), D213 (≠ A211), V227 (≠ I224)

6dz3A Crystal structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase in complex with (3r,4s)-1-((4-amino-5h-pyrrolo[3,2- d]pyrimidin-7-yl)methyl)-4-(((3-(1-butyl-1h-1,2,3-triazol-4-yl) propyl)thio)methyl)pyrrolidin-3-ol (see paper)
32% identity, 96% coverage: 6:239/245 of query aligns to 6:243/273 of 6dz3A

query
sites
6dz3A

I

I

G

G

T
|
T

G

G

L

L

T

D

Q

D

L

P

E

E

G

I

L

L

S

E

I

G

R

R

Q

T

S

E

L

K

A

Y

L

V

D

D

T

T

P

P

Y

F

G

G

A

K

P
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P

S

S

A

D

E

A

I

L

Q

I

V

L

G

G

E

K

Y

I

A

K

G

N

R

V

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D

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C

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V

F

L

L

L

A

A

R

R

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H

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G

H

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Q

H
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H

R

T

F
x
I

P
x
M

P

P

H

S

Q

K

V

V

N

N

Y

Y

R

Q

A

A

N

N

L

I

W

W

A

A

L

L

K

K

Q

E

A

E

G

G

A

C

Q

T

A

H

I

V

L

I

A

V

V
x
T

N

T

A
|
A

V
x
C

G
|
G

G

S

I

L

H

R

A

E

M

I

G

Q

T

P

G

G

H

D

F

I

C

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V

I

P

I

H

D

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Q

L

F

I

I

D

D

Y

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T

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S

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M

R

R

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H

Q

T

S

Y

F

F

Y

A

D

D

G

D

S

-

H

-

S

-

C

L

A

E

R

Q

G

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V

T

C

H

H

I

I

D

P

F

M

S

A

Y

E

P

P

Y

F

S

C

E

P

P

K

L

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R

R

E

E

Q

V

L

L

I

I

A

E

A

T

L

A

A

K

E

L

G

G

C

L

A

R

Y

C

S

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S

S

H

K

G

G

V

T

Y

M

A

V

C

T

T

I

Q

E

G

G

P

P

R

R

L
x
F

E

S

T

S

V

R

A

A

E

E

I

S

A

F

R

M

L

F

E

R

R

T

D

W

G

G

C

A

D

D

I

V

V
x
I

G
x
N

M
|
M

T
|
T

G

T

M

V

P

P

E

E

A

V

L

L

A

A

R

K

E

E

L

A

E

G

L

I

D

C

Y

Y

A

A

C

S

L

I

A

A

L

M

V

A

V

T

N
x
D

P

Y

A
x
D

A

C

G

W

K

K

S

E

-

H

T

E

A

E

V

A

I

V

T

S

M
x
V

A

D

E

R

I
x
V

E

L

Q

K

A

T

L
|
L

H

K

E

E

G

N

M

A

G

N

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K

V

A

K

K

S

S

T

L

L

L

active site: T10 (= T10), P33 (= P33), H57 (= H57), I59 (≠ F59), M60 (≠ P60), A86 (= A86), M188 (= M185), T189 (= T186), D212 (≠ N209), D214 (≠ A211), V225 (≠ M221)
binding (3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-({[3-(1-butyl-1H-1,2,3-triazol-4-yl)propyl]sulfanyl}methyl)pyrrolidin-3-ol: T84 (≠ V84), A86 (= A86), C87 (≠ V87), G88 (= G88), F169 (≠ L166), I186 (≠ V183), N187 (≠ G184), M188 (= M185), D212 (≠ N209), D214 (≠ A211), V228 (≠ I224), L232 (= L228)

6dyzA Crystal structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase in complex with (3r,4s)-1-((4-amino-5h-pyrrolo[3,2- d]pyrimidin-7-yl)methyl)-4-((prop-2-yn-1-ylthio)methyl)pyrrolidin-3- ol (see paper)
32% identity, 96% coverage: 6:239/245 of query aligns to 6:243/273 of 6dyzA

query
sites
6dyzA

I

I

G

G

G
|
G

T
|
T

G

G

L

L

T

D

Q

D

L

P

E

E

G

I

L

L

S

E

I

G

R

R

Q

T

S

E

L

K

A

Y

L

V

D

D

T

T

P

P

Y

F

G

G

A

K

P
|
P

S

S

A

D

E

A

I

L

Q

I

V

L

G

G

E

K

Y

I

A

K

G

N

R

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E

D

V

C

L

V

F

L

L

L

A

A

R
|
R

H
|
H

G

G

H

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P

Q

H
|
H

R

T

F
x
I

P
x
M

P

P

H

S

Q

K

V

V

N

N

Y

Y

R

Q

A

A

N

N

L

I

W

W

A

A

L

L

K

K

Q

E

A

E

G

G

A

C

Q

T

A

H

I

V

L

I

A

V

V

T

N
x
T

A
|
A

V
x
C

G
|
G

G

S

I

L

H

R

A

E

M

I

G

Q

T

P

G

G

H

D

F

I

C

V

V

I

P

I

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D

Q

Q

L

F

I

I

D

D

Y

R

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T

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M

R

R

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P

H

Q

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S

Y

F

F

Y

A

D

D

G

D

S

-

H

-

S

-

C

L

A

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Q

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V

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C

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H

I

I

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F

M

S

A

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F

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P

K

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R

R

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E

Q

V

L

L

I

I

A

E

A

T

L

A

A

K

E

L

G

G

C

L

A

R

Y

C

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S

S

H

K

G

G

V

T

Y

M

A

V

C

T

T

I

Q

E

G

G

P

P

R

R

L
x
F

E

S

T

S

V

R

A

A

E

E

I

S

A

F

R

M

L

F

E

R

R

T

D

W

G

G

C

A

D

D

I

V

V
x
I

G
x
N

M
|
M

T
|
T

G

T

M

V

P

P

E

E

A

V

L

L

A

A

R

K

E

E

L

A

E

G

L

I

D

C

Y

Y

A

A

C

S

L

I

A

A

L

M

V

A

V

T

N
x
D

P

Y

A
x
D

A

C

G

W

K

K

S

E

-

H

T

E

A

E

V

A

I

V

T

S

M
x
V

A

D

E

R

I
x
V

E

L

Q

K

A

T

L

L

H

K

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E

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N

M

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K

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A

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K

S

S

T

L

L

L

active site: T10 (= T10), P33 (= P33), H57 (= H57), I59 (≠ F59), M60 (≠ P60), A86 (= A86), M188 (= M185), T189 (= T186), D212 (≠ N209), D214 (≠ A211), V225 (≠ M221)
binding (3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-{[(prop-2-yn-1-yl)sulfanyl]methyl}pyrrolidin-3-ol: T10 (= T10), H57 (= H57), A86 (= A86), C87 (≠ V87), G88 (= G88), F169 (≠ L166), I186 (≠ V183), N187 (≠ G184), M188 (= M185), D212 (≠ N209), D214 (≠ A211), V228 (≠ I224)
binding phosphate ion: G9 (= G9), T10 (= T10), R52 (= R52), H53 (= H53), T85 (≠ N85), A86 (= A86), T189 (= T186)

5tc8A Crystal structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase in complex with methylthio-dadme-immucillin-a
32% identity, 96% coverage: 6:239/245 of query aligns to 6:243/273 of 5tc8A

query
sites
5tc8A

I

I

G

G

T
|
T

G

G

L

L

T

D

Q

D

L

P

E

E

G

I

L

L

S

E

I

G

R

R

Q

T

S

E

L

K

A

Y

L

V

D

D

T

T

P

P

Y

F

G

G

A

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P
|
P

S

S

A

D

E

A

I

L

Q

I

V

L

G

G

E

K

Y

I

A

K

G

N

R

V

E

D

V

C

L

V

F

L

L

L

A

A

R

R

H

H

G

G

H

R

P

Q

H
|
H

R

T

F
x
I

P
x
M

P

P

H

S

Q

K

V

V

N

N

Y

Y

R

Q

A

A

N

N

L

I

W

W

A

A

L

L

K

K

Q

E

A

E

G

G

A

C

Q

T

A

H

I

V

L

I

A

V

V

T

N

T

A
|
A

V
x
C

G
|
G

G

S

I

L

H

R

A

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M

I

G

Q

T

P

G

G

H

D

F

I

C

V

V

I

P

I

H

D

Q

Q

L

F

I

I

D

D

Y

R

T

T

S

T

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M

R

R

Q

P

H

Q

T

S

Y

F

F

Y

A

D

D

G

D

S

-

H

-

S

-

C

L

A

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R

Q

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V

T

C

H

H

I

I

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P

F

M

S

A

Y

E

P

P

Y

F

S

C

E

P

P

K

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R

R

E

E

Q

V

L

L

I

I

A

E

A

T

L

A

A

K

E

L

G

G

C

L

A

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Y

C

S

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S

S

H

K

G

G

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T

Y

M

A

V

C

T

T

I

Q

E

G

G

P

P

R

R

L
x
F

E

S

T

S

V

R

A

A

E

E

I

S

A

F

R

M

L

F

E

R

R

T

D

W

G

G

C

A

D

D

I

V

V
x
I

G
x
N

M
|
M

T
|
T

G

T

M

V

P

P

E

E

A

V

L

L

A

A

R

K

E

E

L

A

E

G

L

I

D

C

Y

Y

A

A

C

S

L

I

A

A

L

M

V

A

V
x
T

N
x
D

P

Y

A
x
D

A

C

G

W

K

K

S

E

-

H

T

E

A

E

V

A

I

V

T

S

M
x
V

A

D

E

R

I
x
V

E

L

Q

K

A

T

L

L

H

K

E

E

G

N

M

A

G

N

K

K

V

A

K

K

S

S

T

L

L

L

active site: T10 (= T10), P33 (= P33), H57 (= H57), I59 (≠ F59), M60 (≠ P60), A86 (= A86), M188 (= M185), T189 (= T186), D212 (≠ N209), D214 (≠ A211), V225 (≠ M221)
binding (3r,4s)-1-[(4-amino-5h-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(methylsulfanyl)methyl]pyrrolidin-3-ol: T10 (= T10), A86 (= A86), C87 (≠ V87), G88 (= G88), F169 (≠ L166), I186 (≠ V183), N187 (≠ G184), M188 (= M185), T211 (≠ V208), D212 (≠ N209), D214 (≠ A211), V228 (≠ I224)

5tc6A Crystal structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase in complex with propylthio-immucillin-a
32% identity, 96% coverage: 6:239/245 of query aligns to 6:243/273 of 5tc6A

query
sites
5tc6A

I

I

G

G

T
|
T

G

G

L

L

T

D

Q

D

L

P

E

E

G

I

L

L

S

E

I

G

R

R

Q

T

S

E

L

K

A

Y

L

V

D

D

T

T

P

P

Y

F

G

G

A

K

P
|
P

S

S

A

D

E

A

I

L

Q

I

V

L

G

G

E

K

Y

I

A

K

G

N

R

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E

D

V

C

L

V

F

L

L

L

A

A

R

R

H

H

G

G

H

R

P

Q

H
|
H

R

T

F
x
I

P
x
M

P

P

H

S

Q

K

V

V

N

N

Y

Y

R

Q

A

A

N

N

L

I

W

W

A

A

L

L

K

K

Q

E

A

E

G

G

A

C

Q

T

A

H

I

V

L

I

A

V

V

T

N

T

A
|
A

V
x
C

G
|
G

G

S

I

L

H

R

A

E

M

I

G

Q

T

P

G

G

H

D

F

I

C

V

V

I

P

I

H

D

Q

Q

L

F

I

I

D

D

Y

R

T

T

S

T

G

M

R

R

Q

P

H

Q

T

S

Y

F

F

Y

A

D

D

G

D

S

-

H

-

S

-

C

L

A

E

R

Q

G

V

V

T

C

H

H

I

I

D

P

F

M

S

A

Y

E

P

P

Y

F

S

C

E

P

P

K

L

T

R

R

E

E

Q

V

L

L

I

I

A

E

A

T

L

A

A

K

E

L

G

G

C

L

A

R

Y

C

S

H

S

S

H

K

G

G

V

T

Y

M

A

V

C

T

T

I

Q

E

G

G

P

P

R

R

L
x
F

E

S

T

S

V

R

A

A

E

E

I

S

A

F

R

M

L

F

E

R

R

T

D

W

G

G

C

A

D

D

I

V

V

I

G
x
N

M
|
M

T
|
T

G

T

M

V

P

P

E

E

A

V

L

L

A

A

R

K

E

E

L

A

E

G

L

I

D

C

Y

Y

A

A

C

S

L

I

A

A

L

M

V

A

V

T

N
x
D

P

Y

A
x
D

A

C

G

W

K

K

S

E

-

H

T

E

A

E

V

A

I

V

T

S

M
x
V

A

D

E

R

I
x
V

E
x
L

Q

K

A

T

L

L

H

K

E

E

G

N

M

A

G

N

K

K

V

A

K

K

S

S

T

L

L

L

active site: T10 (= T10), P33 (= P33), H57 (= H57), I59 (≠ F59), M60 (≠ P60), A86 (= A86), M188 (= M185), T189 (= T186), D212 (≠ N209), D214 (≠ A211), V225 (≠ M221)
binding (2S,3S,4R,5S)-2-(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)-5-[(propylsulfanyl)methyl]pyrrolidine-3,4-diol: T10 (= T10), A86 (= A86), C87 (≠ V87), G88 (= G88), F169 (≠ L166), N187 (≠ G184), M188 (= M185), D212 (≠ N209), V225 (≠ M221), V228 (≠ I224), L229 (≠ E225)

3ozcA Crystal structure of human 5'-deoxy-5'-methyladenosine phosphorylase in complex with pcl-phenylthiodadmeimma
32% identity, 96% coverage: 6:239/245 of query aligns to 6:243/273 of 3ozcA

query
sites
3ozcA

I

I

G

G

T
|
T

G

G

L

L

T

D

Q

D

L

P

E

E

G

I

L

L

S

E

I

G

R

R

Q

T

S

E

L

K

A

Y

L

V

D

D

T

T

P

P

Y

F

G

G

A

K

P
|
P

S

S

A

D

E

A

I

L

Q

I

V

L

G

G

E

K

Y

I

A

K

G

N

R

V

E

D

V

C

L

V

F

L

L

L

A

A

R

R

H

H

G

G

H

R

P

Q

H
|
H

R

T

F
x
I

P
x
M

P

P

H

S

Q

K

V

V

N

N

Y

Y

R

Q

A

A

N

N

L

I

W

W

A

A

L

L

K

K

Q

E

A

E

G

G

A

C

Q

T

A

H

I

V

L

I

A

V

V

T

N

T

A
|
A

V
x
C

G
|
G

G

S

I

L

H

R

A

E

M

I

G

Q

T

P

G

G

H

D

F

I

C

V

V

I

P

I

H

D

Q

Q

L

F

I

I

D

D

Y

R

T

T

S

T

G

M

R

R

Q

P

H

Q

T

S

Y

F

F

Y

A

D

D

G

D

S

-

H

-

S

-

C

L

A

E

R

Q

G

V

V

T

C

H

H

I

I

D

P

F

M

S

A

Y

E

P

P

Y

F

S

C

E

P

P

K

L

T

R

R

E

E

Q

V

L

L

I

I

A

E

A

T

L

A

A

K

E

L

G

G

C

L

A

R

Y

C

S

H

S

S

H

K

G

G

V

T

Y

M

A

V

C

T

T

I

Q

E

G

G

P

P

R

R

L
x
F

E

S

T

S

V

R

A

A

E

E

I

S

A

F

R

M

L

F

E

R

R

T

D

W

G

G

C

A

D

D

I

V

V
x
I

G
x
N

M
|
M

T
|
T

G

T

M

V

P

P

E

E

A

V

L

L

A

A

R

K

E

E

L

A

E

G

L

I

D

C

Y

Y

A

A

C

S

L

I

A

A

L

M

V

A

V

T

N
x
D

P

Y

A
x
D

A

C

G

W

K

K

S

E

-

H

T

E

A

E

V

A

I

V

T

S

M
x
V

A

D

E

R

I
x
V

E
x
L

Q

K

A

T

L

L

H

K

E

E

G

N

M

A

G

N

K

K

V

A

K

K

S

S

T

L

L

L

active site: T10 (= T10), P33 (= P33), H57 (= H57), I59 (≠ F59), M60 (≠ P60), A86 (= A86), M188 (= M185), T189 (= T186), D212 (≠ N209), D214 (≠ A211), V225 (≠ M221)
binding (3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-{[(4-chlorophenyl)sulfanyl]methyl}pyrrolidin-3-ol: T10 (= T10), H57 (= H57), A86 (= A86), C87 (≠ V87), G88 (= G88), F169 (≠ L166), I186 (≠ V183), N187 (≠ G184), M188 (= M185), D212 (≠ N209), D214 (≠ A211), V228 (≠ I224), L229 (≠ E225)

5tc5A Crystal structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase in complex with butylthio-dadme-immucillin-a and chloride
32% identity, 96% coverage: 6:239/245 of query aligns to 19:256/286 of 5tc5A

query
sites
5tc5A

I

I

G

G

T
|
T

G

G

L

L

T

D

Q

D

L

P

E

E

G

I

L

L

S

E

I

G

R

R

Q

T

S

E

L

K

A

Y

L

V

D

D

T

T

P

P

Y

F

G

G

A

K

P
|
P

S

S

A

D

E

A

I

L

Q

I

V

L

G

G

E

K

Y

I

A

K

G

N

R

V

E

D

V

C

L

V

F

L

L

L

A

A

R

R

H

H

G

G

H

R

P

Q

H
|
H

R

T

F
x
I

P
x
M

P

P

H

S

Q

K

V

V

N

N

Y

Y

R

Q

A

A

N

N

L

I

W

W

A

A

L

L

K

K

Q

E

A

E

G

G

A

C

Q

T

A

H

I

V

L

I

A

V

V

T

N

T

A
|
A

V
x
C

G
|
G

G

S

I

L

H

R

A

E

M

I

G

Q

T

P

G

G

H

D

F

I

C

V

V

I

P

I

H

D

Q

Q

L

F

I

I

D

D

Y

R

T

T

S

T

G

M

R

R

Q

P

H

Q

T

S

Y

F

F

Y

A

D

D

G

D

S

-

H

-

S

-

C

L

A

E

R

Q

G

V

V

T

C

H

H

I

I

D

P

F

M

S

A

Y

E

P

P

Y

F

S

C

E

P

P

K

L

T

R

R

E

E

Q

V

L

L

I

I

A

E

A

T

L

A

A

K

E

L

G

G

C

L

A

R

Y

C

S

H

S

S

H

K

G

G

V

T

Y

M

A

V

C

T

T

I

Q

E

G

G

P

P

R

R

L
x
F

E

S

T

S

V

R

A

A

E

E

I

S

A

F

R

M

L

F

E

R

R

T

D

W

G

G

C

A

D

D

I

V

V
x
I

G

N

M
|
M

T
|
T

G

T

M

V

P

P

E

E

A

V

L

L

A

A

R

K

E

E

L

A

E

G

L

I

D

C

Y

Y

A

A

C

S

L

I

A

A

L

M

V

A

V

T

N
x
D

P

Y

A
x
D

A

C

G

W

K

K

S

E

-

H

T

E

A

E

V

A

I

V

T

S

M
x
V

A

D

E

R

I
x
V

E

L

Q

K

A

T

L

L

H

K

E

E

G

N

M

A

G

N

K

K

V

A

K

K

S

S

T

L

L

L

active site: T23 (= T10), P46 (= P33), H70 (= H57), I72 (≠ F59), M73 (≠ P60), A99 (= A86), M201 (= M185), T202 (= T186), D225 (≠ N209), D227 (≠ A211), V238 (≠ M221)
binding (3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(butylsulfanyl)methyl]pyrrolidin-3-ol: A99 (= A86), C100 (≠ V87), G101 (= G88), F182 (≠ L166), I199 (≠ V183), M201 (= M185), D225 (≠ N209), D227 (≠ A211), V241 (≠ I224)

1sd1A Structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase complexed with formycin a (see paper)
32% identity, 96% coverage: 6:239/245 of query aligns to 6:238/268 of 1sd1A

query
sites
1sd1A

I

I

G

G

T
|
T

G

G

L

L

T

D

Q

D

L

P

E

E

G

I

L

L

S

E

I

G

R

R

Q

T

S

E

L

K

A

Y

L

V

D

D

T

T

P

P

Y

F

G

G

A

K

P
|
P

S

S

A

D

E

A

I

L

Q

I

V

L

G

G

E

K

Y

I

A

K

G

N

R

V

E

D

V

C

L

V

F

L

L

L

A

A

R

R

H

H

G

G

H

R

P

Q

H
|
H

R

T

F
x
I

P
x
M

P

P

H

S

Q

K

V

V

N

N

Y

Y

R

Q

A

A

N

N

L

I

W

W

A

A

L

L

K

K

Q

E

A

E

G

G

A

C

Q

T

A

H

I

V

L

I

A

V

V

T

N

T

A
|
A

V
x
C

G
|
G

G

S

I

L

H

R

A

E

M

I

G

Q

T

P

G

G

H

D

F

I

C

V

V

I

P

I

H

D

Q

Q

L

F

I

I

D

D

Y

R

T

T

S

T

G

M

R

R

Q

P

H

Q

T

S

Y

F

F

Y

A

D

D

G

D

S

-

H

-

S

-

C

L

A

E

R

Q

G

V

V

T

C

H

H

I

I

D

P

F

M

S

A

Y

E

P

P

Y

F

S

C

E

P

P

K

L

T

R

R

E

E

Q

V

L

L

I

I

A

E

A

T

L

A

A

K

E

L

G

G

C

L

A

R

Y

C

S

H

S

S

H

K

G

G

V

T

Y

M

A

V

C

T

T

I

Q

E

G

G

P

P

R

R

L
x
F

E

S

T

S

V

R

A

A

E

E

I

S

A

F

R

M

L

F

E

R

R

T

D

W

G

G

C

A

D

D

I

V

V
x
I

G
x
N

M
|
M

T
|
T

G

T

M

V

P

P

E

E

A

V

L

L

A

A

R

K

E

E

L

A

E

G

L

I

D

C

Y

Y

A

A

C

S

L

I

A

A

L

M

V

-

N

-

P

-

A

A

A

T

G
x
D

K

Y

S
x
D

T

C

A

W

V

A

I

V

T

S

M
x
V

A

D

E

R

I

V

E

L

Q

K

A

T

L

L

H

K

E

E

G

N

M

A

G

N

K

K

V

A

K

K

S

S

T

L

L

L

active site: T10 (= T10), P33 (= P33), H57 (= H57), I59 (≠ F59), M60 (≠ P60), A86 (= A86), M188 (= M185), T189 (= T186), D212 (≠ G213), D214 (≠ S215), V220 (≠ M221)
binding (1S)-1-(7-amino-1H-pyrazolo[4,3-d]pyrimidin-3-yl)-1,4-anhydro-D-ribitol: A86 (= A86), C87 (≠ V87), G88 (= G88), F169 (≠ L166), I186 (≠ V183), N187 (≠ G184), M188 (= M185), D212 (≠ G213), D214 (≠ S215)

1cg6A Structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase complexed with 5'-deoxy-5'-methylthioadenosine and sulfate at 1.7 a resolution (see paper)
32% identity, 96% coverage: 6:239/245 of query aligns to 6:238/268 of 1cg6A

query
sites
1cg6A

I

I

G

G

G
|
G

T
|
T

G

G

L

L

T

D

Q

D

L

P

E

E

G

I

L

L

S

E

I

G

R

R

Q

T

S

E

L

K

A

Y

L

V

D

D

T

T

P

P

Y

F

G

G

A

K

P
|
P

S

S

A

D

E

A

I

L

Q

I

V

L

G

G

E

K

Y

I

A

K

G

N

R

V

E

D

V

C

L

V

F

L

L

L

A

A

R
|
R

H
|
H

G

G

H

R

P

Q

H
|
H

R

T

F
x
I

P
x
M

P

P

H

S

Q

K

V

V

N

N

Y

Y

R

Q

A

A

N

N

L

I

W

W

A

A

L

L

K

K

Q

E

A

E

G

G

A

C

Q

T

A

H

I

V

L

I

A

V

V

T

N
x
T

A
|
A

V
x
C

G
|
G

G

S

I

L

H

R

A

E

M

I

G

Q

T

P

G

G

H

D

F

I

C

V

V

I

P

I

H

D

Q

Q

L

F

I

I

D

D

Y

R

T

T

S

T

G

M

R

R

Q

P

H

Q

T

S

Y

F

F

Y

A

D

D

G

D

S

-

H

-

S

-

C

L

A

E

R

Q

G

V

V

T

C

H

H

I

I

D

P

F

M

S

A

Y

E

P

P

Y

F

S

C

E

P

P

K

L

T

R

R

E

E

Q

V

L

L

I

I

A

E

A

T

L

A

A

K

E

L

G

G

C

L

A

R

Y

C

S

H

S

S

H

K

G

G

V

T

Y

M

A

V

C

T

T

I

Q

E

G

G

P

P

R

R

L
x
F

E

S

T

S

V

R

A

A

E

E

I

S

A

F

R

M

L

F

E

R

R

T

D

W

G

G

C

A

D

D

I

V

V

I

G
x
N

M
|
M

T
|
T

G

T

M

V

P

P

E

E

A

V

L

L

A

A

R

K

E

E

L

A

E

G

L

I

D

C

Y

Y

A

A

C

S

L

I

A

A

L

M

V

-

N

-

P

-

A

A

A

T

G
x
D

K

Y

S
x
D

T

C

A

W

V

A

I

V

T

S

M
x
V

A

D

E

R

I
x
V

E

L

Q

K

A

T

L

L

H

K

E

E

G

N

M

A

G

N

K

K

V

A

K

K

S

S

T

L

L

L

active site: T10 (= T10), P33 (= P33), H57 (= H57), I59 (≠ F59), M60 (≠ P60), A86 (= A86), M188 (= M185), T189 (= T186), D212 (≠ G213), D214 (≠ S215), V220 (≠ M221)
binding 5'-deoxy-5'-methylthioadenosine: A86 (= A86), C87 (≠ V87), G88 (= G88), F169 (≠ L166), N187 (≠ G184), M188 (= M185), D212 (≠ G213), V223 (≠ I224)
binding sulfate ion: G9 (= G9), T10 (= T10), R52 (= R52), H53 (= H53), T85 (≠ N85), T189 (= T186)

Query Sequence

>AO353_00450 FitnessBrowser__pseudo3_N2E3:AO353_00450
MTVYAIIGGTGLTQLEGLSIRQSLALDTPYGAPSAEIQVGEYAGREVLFLARHGHPHRFP
PHQVNYRANLWALKQAGAQAILAVNAVGGIHAAMGTGHFCVPHQLIDYTSGRQHTYFADD
LEQVTHIDFSYPYSEPLREQLIAALAAEGCAYSSHGVYACTQGPRLETVAEIARLERDGC
DIVGMTGMPEAALARELELDYACLALVVNPAAGKSTAVITMAEIEQALHEGMGKVKSTLA
RVLKG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory