SitesBLAST

R257 (= R286) mutation to C: Sodium-independent binding affinity for proline.
C281 (≠ T314) mutation to S: Does not affect proline uptake activity. Confers resistance to N-ethylmaleimide. Na(+)-dependent proline binding activity is similar to wild-type carrier.
C344 (≠ G376) mutation to S: Small decrease in proline uptake activity. Confers resistance to N-ethylmaleimide. Exhibits low Na(+)-dependent proline binding.
C349 (≠ G381) mutation to S: Does not affect proline uptake activity. Sensitive to N-ethylmaleimide. Na(+)-dependent proline binding activity is similar to wild-type carrier.
R376 (≠ K409) mutation R->E,Q: No change in activity.; mutation to K: Loss of activity.

5nv9A Substrate-bound outward-open state of a na+-coupled sialic acid symporter reveals a novel na+-site (see paper)
22% identity, 54% coverage: 44:339/552 of query aligns to 13:312/480 of 5nv9A

query
sites
5nv9A

F

Y

V

L

G

A

A

A

T

M

L

L

C

L

I

V

T

G

Y

V

W

Y

A

F

S

S

K

K

R

R

N

Q

K

K

S

T

A

A

A

D

D

D

Y

Y

Y

F

A

R

A

G

G

G

R

R

I

V

T

P

G

G

L

W

Q

A

N

A

G

G

L

V

A

S

I

V

A

F

G
x
A

D
x
T

Y
x
T

M
x
L

S
|
S

A

S

A
x
I

S
x
T

F

F

L

M

G

S

I

I

S

P

A

A

L

K

V

A

F

Y

A

T

S

S

G

D

Y

W

D

T

G

F

L

I

I

I

Y
x
G

S
x
Q

I

Y

G

L

F

A

L

I

V

A

G

I

W

L

P

P

I

L

I

V

L

F

F

Y

L

F

I

Y

A

I

E

P

R

F

L

F

R

R

N

K

L

L

G

-

K

K

Y

I

T

T

F

S

A

A

-

Y

-

E

-

Y

-

L

-

E

-

A

-

R

-

F

D

D

V

V

A

R

S

S

Y

-

R

R

L

L

G

F

Q

A

T

S

Q

L

I

S

R

F

T

M

L

L

S

F

A

H

C

I

G

G

S
x
R

L

V

V

A

V

I

A

T

F

Y

Y

L

L

T

I

V

A

L

Q

A

M

L

V

-

G

-

A

-

G

-

K

-

L

-

I

-

Q

R

L

P

L

F

F

M

G

G

L

I

D

D

Y

P

H

V

V

V

A

L

V

I

I

V

L

L

V

I

G

S

I

L

L

L

M

C

C

I

L

I

Y

Y

V

T

L

W

F

M

G

G

M

I

L

E

A

G

T
x
V

T

I

W

W

V

T

Q
x
D

I

V

I

I

K

Q

A

G

V

L

L

L

L

S

S

G

G

G

A

A

S

V

F

L

M

I

A

F

L

I

M

M

V

I

M

C

K

F

H

K

V

V

H

D

F

G

D

G

F

I

N

S

T

E

L

I

F

F

S

T

-

T

-

A

E

Q

A

A

I

D

K

K

V

F

H

F

P

P

K

T

G

T

E

Q

A

W

I

R

M

W

S

S

P

-

G

-

L

W

V

T

K

D

D

S

P

T

I

I

S

P

A

V

F

L

S

M

L

I

G

G

-
x
F

-

L

L

F

A

A

L

N

M

I

F

Q

G

Q

T

F

A

T

G

A

L

S

P

Q

H

D

I

V

L

V

M

Q

R

R

F

Y

F

I

T

V

V

T

S

D

D

S

A

I

K

K

E

E

A

T

R

K

K

R

S

T

V

L

L

I

Y

T

A

N

T

A

G

K

F

L

I
x
V

G

A

Y

I

F

I

Y

P

I

I

L

F

T

F

F

F

I

A

I

I

G

G

F

S

G

A

A

L

I

F

L

V

L

Y

V

Y

S

Q

T

Q

N

N

P

P

A

S

F

L

K

L

D

P

A

A

-

G

-

F

-

N

T

T

G

G

A

G

L

I

L

L

binding sodium ion: A52 (≠ G83), T53 (≠ D84), L55 (≠ M86), S56 (= S87), V174 (≠ T206), D178 (≠ Q210)
binding N-acetyl-beta-neuraminic acid: T54 (≠ Y85), L55 (≠ M86), S56 (= S87), I58 (≠ A89), T59 (≠ S90), G77 (≠ Y108), Q78 (≠ S109), R131 (≠ S156), F239 (vs. gap), V277 (≠ I307)

Sites not aligning to the query:

binding sodium ion: 335, 338, 338, 339, 341, 342

Q92911 Sodium/iodide cotransporter; Na(+)/I(-) cotransporter; Natrium iodide transporter; Sodium-iodide symporter; Na(+)/I(-) symporter; Solute carrier family 5 member 5 from Homo sapiens (Human) (see 3 papers)
27% identity, 29% coverage: 59:220/552 of query aligns to 41:201/643 of Q92911

query
sites
Q92911

N

Q

K

R

S

S

A

A

A

E

D

D

Y

F

A

T

A

G

G

G

G

R

R

R

I

L

T

A

G

A

L

L

Q

P

N

V

G

G

L

L

A

S

I

L

A

S

G

A

D

S

Y

F

M

M

S

S

A

A

A

V

S

Q

F

V

L

L

G

G

I

V

S

P

A

S

L

E

V

A

F

Y

A

R

S

Y

G

G

Y

L

D

K

G

F

L

L

I

W

Y

M

S

C

I

L

G

G

F

Q

L

L

V

L

G

N

W

S

P

V

I

L

I

T

L
x
A

F

L

L

L

I

F

A

M

E

P

R

V

L

F

R

Y

N

R

L

L

G

G

K

L

Y

T

T

S

F

T

A

Y

D

E

V

Y

A

L

S

E

Y

M

R

R

L

F

G

S

Q

-

T

-

Q

-

I

-

R

R

T

A

L

V

S

R

A

L

C

C

G

G

S

T

L

L

V

Q

V

-

A

-

F

-

Y

Y

L

I

I

V

A

A

Q

T

M

M

V

L

G

Y

A

T

G

G

K

I

L

V

I

I

-

Y

-

A

-

P

-

A

-

L

-

I

-

L

Q

N

L

Q

L

V

F

T

G

G

L

L

D

D

Y

I

H

W

V

A

A

S

V

L

I

L

L

S

V

T

G

G

I

I

L

I

M

C

C

T

L

F

Y

Y

V

T

L

A

F

V

G

G

M

M

L

K

A

A

T

V

W

W

V

T

Q

D

I

V

I

F

K

Q

A

V

V

V

L

V

L

M

L

L

S

S

G

G

A102 (≠ L120) natural variant: A -> P

Sites not aligning to the query:

226 mutation H->A,D,E,K: Significant loss of iodide transport activity but no effect on its localization to the cell membrane.
237 D→A: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization.
242 Required for homodimerization; Y→A: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization. Reduced homodimerization; when associated with A-471. Loss of iodide transport activity; when associated with F-535.
243 Required for homodimerization; T→A: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization. Reduced homodimerization; when associated with A-471.
471 Required for homodimerization; Q→A: No effect on localization to the cell membrane, iodide transport activity and homodimerization. Significant loss of homodimerization; when associated with A-242 or A243.
525 A→F: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization. Loss of iodide transport activity; when associated with A-242.
536 T → Q: requires 2 nucleotide substitutions
556 S → Q: requires 2 nucleotide substitutions

P11170 Sodium/glucose cotransporter 1; Na(+)/glucose cotransporter 1; High affinity sodium-glucose cotransporter; Solute carrier family 5 member 1 from Oryctolagus cuniculus (Rabbit) (see 2 papers)
19% identity, 82% coverage: 27:480/552 of query aligns to 18:501/662 of P11170

query
sites
P11170

E

E

V

S

H

Y

K

E

Q

R

P

I

L

R

N

N

V

A

S

A

A

D

I

I

L

S

M

V

F

I

V

V

V

I

F

Y

V

F

G

L

A

V

T

V

L

M

C

A

I

V

T

G

Y

L

W

W

A

A

-

M

-

F

S

S

K

T

R

N

N

R

K

G

S

T

A

V

A

G

D

G

Y

F

A

L

A

A

G

G

G

R

R

S

I

M

T

V

G

W

L

W

Q

P

N

I

G

G

L

A

A

S

I

L

A

F

G

A

D

S

Y

N

M

I

S

G

A

S

A

G

S

H

F

F

L

V

G

G

I

L

S

A

A

G

L

T

V

G

F

A

A

A

S

S

G

G

-

I

-

A

-

T

-

G

-

F

-

E

Y

W

D

N

G

A

L

L

I

I

Y

M

S

V

I

V

G

-

F

-

L

V

V

L

G

G

W

W

P

V

I

F

I

V

L

P

F

I

L

Y

I

I

A

-

E

-

R

-

L

-

R

-

N

R

L

A

G

G

K

V

Y

V

T

T

F

M

A

P

D

E

V

Y

A

L

S

Q

Y

K

R

R

L

F

G

G

Q

G

T

K

Q

R

I

I

R

Q

T

I

L

Y

S

L

A

S

C

I

G

L

S

S

L

L

V

L

V

Y

A

I

F

F

Y

T

L

K

I

I

A

S

Q

A

M

D

V

I

G

F

A

S

G

G

K

A

L

I

-

F

I

I

Q

Q

L

L

L

T

F

L

G

G

L

L

D

D

Y

I

H

Y

V

V

A

A

V

I

I

I

L

I

V

L

G

L

I

V

L

I

M

T

C

G

L

L

Y

Y

V

T

L

I

F

T

G

G

M

L

L

A

A

A

T

V

T

I

W

Y

V

T

Q

D

I

T

I

L

K

Q

A

T

V

A

L

I

L

M

S

V

G

G

A

S

S

V

F

I

M

L

A

T

L

G

M

F

V

A

M

F

K

H

H

E

V

V

-

G

H

Y

F

E

D

A

F

F

N

T

T

E

L

K

F

Y

S

M

E

R

A

A

I

I

K

P

V

S

H

Q

-

I

-

S

-

Y

-

G

-

N

-

T

-

S

-

I

-

P

-

Q

-

K

-
x
C

-

Y

-

T

P

P

K

R

G

E

E

D

A

A

I

F

M

H

S

-

P

-

G

-

L

I

V

F

K

R

D

D

P

A

I

I

S

T

A

G

F

D

S

I

L

P

G

W

L

P

A

G

L

L

M

V

F

F

G

G

T

M

A

S

G

I

L

L

-

T

-

L

-

W

-

Y

-

W

-

C

-

T

-

D

P

Q

H

V

I

I

L

V

M

Q

R

R

F

C

F

L

T

S

V

A

S

K

D

N

A

L

K

S

E

H

A

V

R

K

K

A

S

G

V

C

L

I

Y

-

A

-

T

-

G

-

F

L

I

C

G

G

Y

Y

F

L

Y

K

I

V

-

M

-

P

-

M

-

F

L

L

T

I

F

V

I

M

G

G

F

M

G

V

A

S

I

R

L

I

L

L

V

Y

S

T

T

D

N

K

P

V

A

A

-

C

-

V

-

P

-

S

-

E

F

C

K

E

D

R

A

Y

T

C

G

G

A

T

L

R

L

V

G

G

G

C

N

T

N

N

M

I

A

A

A

F

V

P

H

T

L

L

A

V

N

V

A

E

V

L

G

M

G

P

S

N

I

G

F

L

L

R

G

G

F

L

I

M

S

L

A

S

V

V

A

M

F

M

A

A

T

S

I

L

L

M

A

S

V

S

V

L

A

T

G

S

L

I

T

F

L

N

A

S

G

A

A

S

T

T

A

L

V

F

S

T

H

M

D

D

L

I

Y

Y

A

T

S

K

V

I

I

R

K

K

K

-

G

-

K

K

A

A

N

S

E

E

K

K

D

E

E

L

I

M

R

I

V

A

S

G

K

R

I

L

T

F

T

M

I

L

A

F

L

L

G

I

V

G

L

I

A

S

I

I

V

A

L

W

G

V

I

P

L

I

F

V

E

Q

S

S

Q

A

N

Q

I

S

A

G

F

Q

M

L

V

F

G

D

L

Y

A

I

F
x
Q

S

S

I

I

A
x
T

A

S

S

Y

C

L

N

G

F

P

P

P

-

I

-

A

V

V

L

F

L

L

S

A

M

I

Y

F

W

W

K

K

K

R

L

V

T

N

T

E

R

P

G

G

A

A

M

F

V

W

G

G

G

L

W

V

M

L

G

G

L

F

V

L

S

I

A

G

V

I

G

S

L

R

M

M

V

I

C255 (vs. gap) modified: Disulfide link with 608
Q457 (≠ F439) mutation to W: Drasticly decreased affinity for glucose and phlorizin.
T460 (≠ A442) mutation to W: Decreased affinity for glucose and phlorizin.

Sites not aligning to the query:

608 modified: Disulfide link with 255

7sl8A Cryoem structure of sglt1 at 3.4 a resolution (see paper)
21% identity, 61% coverage: 131:464/552 of query aligns to 92:453/582 of 7sl8A

query
sites
7sl8A

G

G

K

V

Y

V

T

T

F

M

A

P

D

E

V

Y

A

L

S

R

Y

K

R

R

L

F

G

G

Q

G

T

Q

Q

R

I

I

R

Q

T

V

L

Y

S

L

A

S

C

V

G

L

S

S

L

L

V

F

V

L

V

Y

A

I

F

F

Y

T

L

K

I

I

A

S

Q

V

M

D

V

I

G

F

A

S

G

G

K

A

L

I

-

F

I

I

Q

N

L

L

L

A

F

L

G

G

L

W

D

N

Y

L

H

Y

V

L

A

S

V

I

I

I

L

L

V

L

G

L

I

A

L

I

M

T

C

A

L

L

Y

Y

V

T

L

I

F

T

G

G

M

L

L

A

A

A

T

V

T

I

W

Y

V

T

Q

D

I

T

I

L

K

Q

A

T

V

L

L

I

L

M

L

L

S

I

G

G

A

A

-

L

-

I

-

L

-

M

-

G

-

F

-

A

-

F

-

H

-

E

-

V

-

G

-

Y

-

D

S

A

F

F

M

M

A

E

-

K

-

Y

L

M

M

K

V

A

M

I

K

P

H

T

V

I

H

V

F

S

D

D

F

G

N

N

T

T

L

T

F

F

S

Q

E

E

A

K

I

C

K

Y

V

T

H

-

P

P

K

R

G

A

E

D

A

S

I

F

M

-

S

-

P

-

G

-

G

H

L

I

V

F

K

R

D

D

P

P

I

L

S

T

A

G

-

D

-

L

-

P

-

W

-

P

-

G

-

F

-

I

F

F

S

G

L

L

G

T

L

I

A

L

L

A

M

L

F

W

G

Y

T

W

A

C

G

T

L

D

P

Q

H

V

I

I

L

V

M

Q

R

R

F

C

F

L

T

A

V

A

S

K

D

N

A

M

K

S

E

H

A

V

R

K

K
x
G

S

G

V

C

L

I

Y

L

A

A

T

-

G

-

F

-

I

-

G

G

Y
|
Y

F

L

Y

K

I

L

L

L

T

P

F

M

I

F

I

I

-

M

-

V

-

M

-

P

G

G

F

M

G

I

A

S

I

R

L

I

L

L

V

F

S

P

T

D

N

K

P

V

A

A

F

C

-

V

-

P

-

S

-

E

-

C

K

E

D

K

A

Y

T

C

G

G

A

T

L

K

L

V

G

G

G

C

N

T

N

N

M

I

A

A

A

Y

V

P

H

T

L

L

A

V

N

V

A

E

V

L

G

M

G

P

S

N

I

G

F

L

L

R

G

G

F

L

I

M

S

L

A

A

V

V

A

M

F

L

A

A

T

A

I

L

L

M

A

S

V

S

V

L

A

T

G

S

L

I

T

F

L

N

A

S

G

A

A

S

T

T

A

L

V

F

S

T

H

M

D

D

L

I

Y

Y

A

A

S

K

V

V

I

R

K

K

K

R

G

-

K

-

A

A

N

S

E

E

K

K

D

E

E

L

I

M

R

I

V

V

S

G

K

R

I

L

T

F

T

V

I

L

A

F

L

L

G

V

V

V

L

V

A

S

I

I

V

A

L

W

G

I

I

P

L

I

F

V

E

Q

S

S

Q

A

N

Q

I

S

A

G

F

Q

M

L

V

F

G

D

L

Y

A

I

F

Q

S

S

I

V

A

S

S

Y

C

L

N

A

F

P

P

P

V

V

-

A

-

V

L

F

L

L

S

A

M

I

Y

F

W

W

K

K

K

R

L

V

T

N

T

E

R

Q

G

G

A

A

binding cholesterol: G280 (≠ K298), Y287 (= Y309)

Sites not aligning to the query:

binding cholesterol: 35, 551, 554, 555, 558

7slaA Cryoem structure of sglt1 at 3.15 angstrom resolution (see paper)
21% identity, 61% coverage: 131:464/552 of query aligns to 93:454/585 of 7slaA

query
sites
7slaA

G

G

K

V

Y

V

T

T

F

M

A

P

D

E

V

Y

A

L

S

R

Y

K

R

R

L

F

G

G

Q

G

T

Q

Q

R

I

I

R

Q

T

V

L

Y

S

L

A

S

C

V

G

L

S

S

L

L

V

F

V

L

V

Y

A

I

F

F

Y

T

L

K

I

I

A

S

Q

V

M

D

V

I

G

F

A

S

G

G

K

A

L

I

-

F

I

I

Q

N

L

L

L

A

F

L

G

G

L

W

D

N

Y

L

H

Y

V

L

A

S

V

I

I

I

L

L

V

L

G

L

I

A

L

I

M

T

C

A

L

L

Y

Y

V

T

L

I

F

T

G

G

M

L

L

A

A

A

T

V

T

I

W

Y

V

T

Q

D

I

T

I

L

K

Q

A

T

V

L

L

I

L

M

L

L

S

I

G

G

A

A

-

L

-

I

-

L

-

M

-

G

-

F

-

A

-

F

-

H

-

E

-

V

-

G

-

Y

-

D

S

A

F

F

M

M

A

E

-

K

-

Y

L

M

M

K

V

A

M

I

K

P

H

T

V

I

H

V

F

S

D

D

F

G

N

N

T

T

L

T

F

F

S

Q

E

E

A

K

I

C

K

Y

V

T

H

-

P

P

K

R

G

A

E

D

A

S

I

F

M

-

S

-

P

-

G

-

G

H

L

I

V

F

K

R

D

D

P

P

I

L

S

T

A

G

-

D

-

L

-

P

-

W

-

P

-

G

-

F

-

I

F

F

S

G

L

L

G

T

L

I

A

L

L

A

M

L

F

W

G

Y

T

W

A

C

G

T

L

D

P

Q

H

V

I

I

L

V

M

Q

R

R

F

C

F

L

T

A

V

A

S

K

D

N

A

M

K
x
S

E

H

A

V

R

K

K

G

S

G

V

C

L

I

Y

L

A

A

T

-

G

-

F

-

I

-

G

G

Y
|
Y

F

L

Y

K

I

L

L

L

T

P

F

M

I

F

I

I

-

M

-

V

-

M

-

P

G

G

F

M

G

I

A

S

I

R

L

I

L

L

V

F

S

P

T

D

N

K

P

V

A

A

F

C

-

V

-

P

-

S

-

E

-

C

K

E

D

K

A

Y

T

C

G

G

A

T

L

K

L

V

G

G

G

C

N

T

N

N

M

I

A

A

A

Y

V

P

H

T

L

L

A

V

N

V

A

E

V

L

G

M

G

P

S

N

I

G

F

L

L

R

G

G

F

L

I

M

S

L

A

A

V

V

A

M

F

L

A

A

T

A

I

L

L

M

A

S

V

S

V

L

A

T

G

S

L

I

T

F

L

N

A

S

G

A

A

S

T

T

A

L

V

F

S

T

H

M

D

D

L

I

Y

Y

A

A

S

K

V

V

I

R

K

K

K

R

G

-

K

-

A

A

N

S

E

E

K

K

D

E

E

L

I

M

R

I

V

V

S

G

K

R

I

L

T

F

T

V

I

L

A

F

L

L

G

V

V

V

L

V

A

S

I

I

V

A

L

W

G

I

I

P

L

I

F

V

E

Q

S

S

Q

A

N

Q

I

S

A

G

F

Q

M

L

V

F

G

D

L

Y

A

I

F

Q

S

S

I

V

A

S

S

Y

C

L

N

A

F

P

P

P

V

V

-

A

-

V

L

F

L

L

S

A

M

I

Y

F

W

W

K

K

K

R

L

V

T

N

T

E

R

Q

G

G

A

A

binding cholesterol hemisuccinate: S277 (≠ K294), Y288 (= Y309)

Sites not aligning to the query:

binding cholesterol hemisuccinate: 36, 554, 558, 561

P13866 Sodium/glucose cotransporter 1; Na(+)/glucose cotransporter 1; High affinity sodium-glucose cotransporter; Solute carrier family 5 member 1 from Homo sapiens (Human) (see 6 papers)
20% identity, 79% coverage: 27:464/552 of query aligns to 18:485/664 of P13866

query
sites
P13866

E

E

V

T

H

H

K

E

Q

L

P

I

L

R

N

N

V

A

S

A

A

D

I

I

L

S

M

I

F

I

V

V

V

I

F

Y

V

F

G

V

A

V

T

V

L

M

C

A

I

V

T

G

Y

L

W

W

A

A

-

M

-

F

S

S

K

T

R
x
N

N

R

K

G

S

T

A

V

A

G

D

G

Y

F

A

L

A

A

G

G

G

R

R

S

I

M

T

V

G
x
W

L

W

Q

P

N

I

G

G

L

A

A

S

I

L

A

F

G

A

D
x
S

Y

N

M

I

S

G

A

S

A

G

S
x
H

F

F

L

V

G

G

I

L

S

A

A

G

L

T

V

G

F

A

A

A

S

S

G

G

-

I

-

A

-

I

-

G

Y

F

D

E

G

W

L

N

I

A

Y

L

S

V

I

L

G

V

F

V

L

V

V

L

G

G

W

W

P

L

I

F

I

V

L

P

F

I

L

Y

I

I

A

-

E

-

R

-

L

-

R

-

N

K

L

A

G

G

K

V

Y

V

T

T

F

M

A

P

D

E

V

Y

A

L

S

R

Y

K

R
|
R

L

F

G

G

Q

G

T

Q

Q

R

I

I

R

Q

T

V

L

Y

S

L

A

S

C

L

G

L

S

S

L

L

V

L

V

Y

A

I

F

F

Y

T

L

K

I

I

A
x
S

Q

A

M

D

V

I

G

F

A

S

G

G

K
x
A

L

I

-

F

I

I

Q

N

L

L

L

A

F

L

G

G

L

L

D

N

Y

L

H

Y

V

L

A

A

V

I

I

F

L

L

V

L

G

L

I

A

L

I

M

T

C

A

L

L

Y
|
Y

V
x
T

L
x
I

F
x
T

G
|
G

M
x
L

L
x
A

A
|
A

T
x
V

T
x
I

W
x
Y

V
x
T

Q
x
D

I
x
T

I
x
L

K
x
Q

A
x
T

V
|
V

L
x
I

L
x
M

L
|
L

S
x
V

G
|
G

A
x
S

-
x
L

-
x
I

-
x
L

-
x
T

-
x
G

-
x
F

-
x
A

-
x
F

-
x
H

-
x
E

-
x
V

-
x
G

-
x
Y

-
x
D

S
x
A

F
|
F

M
|
M

A
x
E

-
x
K

-
x
Y

L
x
M

M
x
K

V
x
A

M
x
I

K
x
P

H
x
T

V
x
I

H
x
V

F
x
S

D
|
D

F
x
G

N
|
N

T
|
T

L
x
T

F
|
F

S
x
Q

E
|
E

A
x
K

I
x
C

K
x
Y

V
x
T

H

-

P
|
P

K
x
R

G
x
A

E
x
D

A
x
S

I
x
F

M
x
H

S

-

P

-

G

-

L
x
I

V
x
F

K
x
R

D
|
D

P
|
P

I
x
L

S
x
T

A
x
G

F
x
D

S
x
L

L
x
P

G
x
W

L
x
P

A
x
G

L
x
F

M
x
I

F
|
F

G
|
G

T
x
M

A
x
S

G

-

L

-

P

-

H

-

I
|
I

L
|
L

M
x
T

R
x
L

F
x
W

F
x
Y

T
x
W

V
x
C

S
x
T

D
|
D

A
x
Q

K
x
V

E
x
I

A
x
V

R
x
Q

-
x
R

-
x
C

-
x
L

-
x
S

-
x
A

K
|
K

S
x
N

V
x
M

L
x
S

Y
x
H

A
x
V

T
x
K

G
|
G

-
x
G

-
x
C

-
x
I

F
x
L

I
x
C

G
|
G

Y
|
Y

F
x
L

Y
x
K

I
x
L

L
x
M

T
x
P

F
x
M

I
x
F

I
|
I

-
x
M

-
x
V

-
x
M

-
x
P

G
|
G

F
x
M

G
x
I

A
x
S

I
x
R

L
x
I

L
|
L

V
x
Y

S
x
T

T
x
E

N
x
K

P
x
I

A
|
A

-
x
C

-
x
V

-
x
P

-
x
S

-
x
E

F
x
C

K
x
E

D
x
K

A
x
Y

T
x
C

G
|
G

A
x
T

L
x
K

L
x
V

G
|
G

G
x
C

N
x
T

N
|
N

M
x
I

A
|
A

A
x
Y

V
x
P

H
x
T

L
|
L

A
x
V

N
x
V

A
x
E

V
x
L

G
x
M

G
x
P

S
x
N

I
x
G

F
x
L

L
x
R

G
|
G

F
x
L

I
x
M

S
x
L

A
x
S

V
|
V

A
x
M

F
x
L

A
|
A

T
x
S

I
x
L

L
x
M

A
x
S

V
x
S

V
x
L

A
x
T

G
x
S

L
x
I

T
x
F

L
x
N

A
x
S

G
x
A

A
x
S

T
|
T

A
x
L

V
x
F

S
x
T

H
x
M

D
|
D

L
x
I

Y
|
Y

A
|
A

S
x
K

V
|
V

I
x
R

K
|
K

K

-

G

-

K
x
R

A
|
A

N
x
S

E
|
E

K
|
K

D
x
E

E
x
L

I
x
M

R
x
I

V
x
A

S
x
G

K
x
R

I
x
L

T
x
F

T
x
I

I
x
L

A
x
V

L
|
L

G
x
I

V
x
G

L
x
I

A
x
S

I
|
I

V
x
A

L
x
W

G
x
V

I
x
P

L
x
I

F
x
V

E
x
Q

S
|
S

Q
x
A

N
x
Q

I
x
S

A
x
G

F
x
Q

M
x
L

V
x
F

G
x
D

L
x
Y

A
x
I

F
x
Q

S
|
S

I
|
I

A
x
T

A
x
S

S
x
Y

C
x
L

N
x
G

F
x
P

P
|
P

-
x
I

-
x
A

V
|
V

L
x
F

L
|
L

S
x
A

M
x
I

Y
x
F

W
|
W

K
|
K

K
x
R

L
x
V

T
x
N

T
x
E

R
x
P

G
|
G

A
|
A

N51 (≠ R58) to S: in GGM; slightly decreased activity; dbSNP:rs17683011
W67 (≠ G74) mutation to A: Strong reduction in D-glucose transporter activity.
S77 (≠ D84) mutation to A: Loss of activity.
H83 (≠ S90) mutation to L: Acquires D-mannose, D-fructose and L-sorbose transporter activity; when associated with A-287 and C-290.; mutation to Q: Loss of D-glucose transporter activity.
R135 (= R142) to W: in GGM; loss of activity
S159 (≠ A166) to P: in GGM; loss of activity
A166 (≠ K173) to T: in GGM; about 90% reduction in activity
D204 (≠ Q210) mutation to A: Loss of activity.
N248 (= N237) modified: carbohydrate, N-linked (GlcNAc...) asparagine; mutation to Q: Loss of N-glycosylation.
C255 (≠ I244) modified: Disulfide link with 511
W276 (≠ G270) to L: in GGM; about 95% reduction in activity
T287 (≠ M285) mutation to A: Acquires D-mannose, D-fructose and L-sorbose transporter activity; when associated with L-83 and C-290.; mutation to N: Loss of D-glucose transporter activity. Has strict selectivity for D-galactose.; mutation T->S,A: Has normal D-glucose and D-galactose transporter activity.
Y290 (≠ F288) mutation to C: Loss of D-galactose transporter activity. Has strict selectivity for D-glucose. Acquires D-mannose, D-fructose and L-sorbose transporter activity; when associated with A-287 and L-83.
W291 (≠ T289) mutation to A: Loss of D-glucose transporter activity.
C292 (≠ V290) to Y: in GGM; loss of activity; mutation to A: Has no effect on water permeability.
Q295 (≠ A293) to R: in GGM; loss of activity
R300 (vs. gap) to S: in GGM; loss of activity
A304 (vs. gap) to V: in GGM; impairs trafficking to the plasma membrane
K321 (≠ Y311) mutation to Q: Acquires D-mannose and D-allose transporter activity comparable to glucose and galactose.
C345 (vs. gap) modified: Disulfide link with 351
C351 (≠ F331) modified: Disulfide link with 345
C355 (≠ T335) modified: Disulfide link with 361
C361 (≠ G341) modified: Disulfide link with 355
N363 (= N343) mutation to A: Loss of water permeation.
L369 (= L349) to S: in GGM; loss of activity
R379 (≠ L359) to Q: in GGM; loss of activity
A388 (= A368) to V: in GGM; loss of activity
S396 (≠ G376) mutation to A: Loss of activity.
F405 (≠ V385) to S: in GGM; loss of activity
A411 (= A391) to T: in GGM; slightly decreased activity; dbSNP:rs17683430
G426 (≠ S408) to R: in GGM; loss of activity
Q451 (≠ F433) mutation to A: Strong reduction in water permeation.
L452 (≠ M434) mutation to A: Loss of water permeation.
D454 (≠ G436) mutation to A: Has no effect on water permeation.
Q457 (≠ F439) mutation to A: Loss of D-glucose transporter activity.; mutation to C: Strong reduction in D-glucose transporter activity.
T460 (≠ A442) mutation to A: Loss of D-glucose transporter activity.
V470 (= V449) to N: in GGM; requires 2 nucleotide substitutions; about 90% reduction in activity

Sites not aligning to the query:

191:664 natural variant: Missing (in GGM; loss of activity)
379:664 natural variant: Missing (in GGM; loss of activity)
499 R → H: in GGM; impairs trafficking to the plasma membrane; decreases the sugar affinity
511 modified: Disulfide link with 255
517 modified: Disulfide link with 522
522 modified: Disulfide link with 517
615 H → Q: in GGM; slightly decreased activity
641 W→A: Slightly reduced D-glucose transporter activity.
660:661 HA→WG: Loss of D-glucose transporter activity.

7wmvA Structure of human sglt1-map17 complex bound with lx2761 (see paper)
20% identity, 79% coverage: 27:464/552 of query aligns to 1:468/602 of 7wmvA

query
sites
7wmvA

E

E

V

T

H

H

K

E

Q

L

P

I

L

R

N

N

V

A

S

A

A

D

I

I

L

S

M

I

F

I

V

V

V

I

F

Y

V

F

G

V

A

V

T

V

L

M

C

A

I

V

T

G

Y

L

W

W

A

A

-

M

-

F

S

S

K

T

R

N

N

R

K

G

S

T

A

V

A

G

D

G

Y

F

A

L

A

A

G

G

G

R

R

S

I

M

T

V

G

W

L

W

Q

P

N

I

G

G

L

A

A

S

I

L

A

F

G

A

D

S

Y
x
N

M

I

S

G

A

S

A

G

S
x
H

F

F

L

V

G

G

I
x
L

S

A

A

G

L

T

V

G

F

A

A

A

S

S

G

G

-

I

-

A

-
x
I

-

G

Y
x
F

D
x
E

G

W

L

N

I
x
A

Y

L

S

V

I

L

G

V

F

V

L

V

V

L

G

G

W

W

P

L

I

F

I

V

L

P

F

I

L

Y

I

I

A

K

E

A

R

-

L

-

R

-

N

-

L

-

G

G

K

V

Y

V

T

T

F

M

A

P

D

E

V

Y

A

L

S

R

Y

K

R

R

L

F

G

G

Q

G

T

Q

Q

R

I

I

R

Q

T

V

L

Y

S

L

A

S

C

L

G

L

S

S

L

L

V

L

V

Y

A

I

F

F

Y

T

L

K

I

I

A

S

Q

A

M

D

V

I

G

F

A

S

G

G

K

A

L

I

-

F

I

I

Q

N

L

L

L

A

F

L

G

G

L

L

D

N

Y

L

H

Y

V

L

A

A

V

I

I

F

L

L

V

L

G

L

I

A

L

I

M

T

C

A

L

L

Y

Y

V

T

L

I

F

T

G

G

M

L

L

A

A

A

T

V

T

I

W

Y

V

T

Q

D

I

T

I

L

K

Q

A

T

V

V

L

I

L

M

L

L

S

V

G

G

A

S

-

L

-

I

-

L

-

T

-

G

-

F

-

A

-

F

-

H

-

E

-

V

-

G

-

Y

-

D

S

A

F

F

M

M

A

E

-

K

-

Y

L

M

M

K

V

A

M

I

K

P

H

T

V

I

H

V

F

S

D

D

F

G

N

N

T

T

L

T

F

F

S

Q

E

E

A

K

I

C

K

Y

V

T

H

-

P

P

K

R

G

A

E

D

A

S

I

F

M

-

S

-

P

-

G

-

G

H

L

I

V

F

K

R

D

D

P

P

I

L

S

T

A

G

F
x
D

S
x
L

L

P

G

W

L

P

A

G

L

F

M

I

F

F

G

G

T
x
M

A

S

G

-

L

-

P

-

H

-

I

I

L
|
L

M
x
T

R

L

F

W

F
x
Y

T
x
W

V

C

S

T

D

D

A

Q

K

V

E

I

A

V

R

Q

-

R

-

C

-

L

-

S

-

A

K

K

S

N

V

M

L

S

Y

H

A

V

T

K

G

G

-

G

-

C

-

I

F

L

I

C

G

G

Y

Y

F

L

Y
x
K

I

L

L

M

T

P

F

M

I

F

I

I

-

M

-

V

-

M

-

P

G

G

F

M

G

I

A

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I

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L

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L

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S

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T

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N

K

P

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A

A

-

C

-

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E

F

C

K

E

D

K

A

Y

T

C

G

G

A

T

L

K

L

V

G

G

G

C

N

T

N

N

M

I

A

A

A

Y

V

P

H

T

L

L

A

V

N

V

A

E

V

L

G

M

G

P

S

N

I

G

F

L

L

R

G

G

F

L

I

M

S

L

A

S

V

V

A

M

F

L

A

A

T

S

I

L

L

M

A

S

V

S

V

L

A

T

G

S

L

I

T

F

L

N

A

S

G

A

A

S

T

T

A

L

V

F

S

T

H

M

D

D

L

I

Y

Y

A

A

S

K

V

V

I

R

K

K

K

-

G

-

K

R

A

A

N

S

E

E

K

K

D

E

E

L

I

M

R

I

V

A

S

G

K

R

I

L

T

F

T

I

I

L

A

V

L

L

G

I

V

G

L

I

A

S

I

I

V

A

L

W

G

V

I

P

L

I

F

V

E

Q

S

S

Q

A

N

Q

I

S

A

G

F

Q

M

L

V
x
F

G
x
D

L

Y

A

I

F
x
Q

S

S

I

I

A

T

A

S

S

Y

C

L

N

G

F

P

P

P

-

I

-

A

V

V

L

F

L

L

S

A

M

I

Y

F

W

W

K

K

K

R

L

V

T

N

T

E

R

P

G

G

A

A

binding N-[2-(dimethylamino)ethyl]-2-methyl-2-[4-[4-[[2-methyl-5-[(2S,3R,4R,5S,6R)-6-methylsulfanyl-3,4,5-tris(oxidanyl)oxan-2-yl]phenyl]methyl]phenyl]butanoylamino]propanamide: N61 (≠ Y85), H66 (≠ S90), L70 (≠ I94), I81 (vs. gap), F84 (≠ Y103), E85 (≠ D104), A88 (≠ I107), D256 (≠ F267), L257 (≠ S268), M266 (≠ T277), L269 (= L284), T270 (≠ M285), Y273 (≠ F288), W274 (≠ T289), K304 (≠ Y311), F436 (≠ V435), D437 (≠ G436), Q440 (≠ F439)

Sites not aligning to the query:

binding N-[2-(dimethylamino)ethyl]-2-methyl-2-[4-[4-[[2-methyl-5-[(2S,3R,4R,5S,6R)-6-methylsulfanyl-3,4,5-tris(oxidanyl)oxan-2-yl]phenyl]methyl]phenyl]butanoylamino]propanamide: 508

Query Sequence

>AO353_01865 AO353_01865 cation/acetate symporter ActP
MIRRLLAALGIAAFAPAVWAAEALTGEVHKQPLNVSAILMFVVFVGATLCITYWASKRNK
SAADYYAAGGRITGLQNGLAIAGDYMSAASFLGISALVFASGYDGLIYSIGFLVGWPIIL
FLIAERLRNLGKYTFADVASYRLGQTQIRTLSACGSLVVVAFYLIAQMVGAGKLIQLLFG
LDYHVAVILVGILMCLYVLFGGMLATTWVQIIKAVLLLSGASFMALMVMKHVHFDFNTLF
SEAIKVHPKGEAIMSPGGLVKDPISAFSLGLALMFGTAGLPHILMRFFTVSDAKEARKSV
LYATGFIGYFYILTFIIGFGAILLVSTNPAFKDATGALLGGNNMAAVHLANAVGGSIFLG
FISAVAFATILAVVAGLTLAGATAVSHDLYASVIKKGKANEKDEIRVSKITTIALGVLAI
VLGILFESQNIAFMVGLAFSIAASCNFPVLLLSMYWKKLTTRGAMVGGWMGLVSAVGLMV
LGPTIWVQIMGHEKAIFPYEYPALFSMAIAFVGIWFFSITDKSAAADNERALFFPQFVRS
QTGLGASGAVSH

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory