SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AO353_02720 FitnessBrowser__pseudo3_N2E3:AO353_02720 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P16703 Cysteine synthase B; CSase B; O-acetylserine (thiol)-lyase B; OAS-TL B; O-acetylserine sulfhydrylase B; EC 2.5.1.47 from Escherichia coli (strain K12) (see paper)
69% identity, 97% coverage: 7:296/300 of query aligns to 3:292/303 of P16703

query
sites
P16703

T

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N71 (= N75) binding pyridoxal 5'-phosphate
S255 (= S259) binding pyridoxal 5'-phosphate

2bhtA Crystal structure of o-acetylserine sulfhydrylase b (see paper)
69% identity, 97% coverage: 7:296/300 of query aligns to 3:292/294 of 2bhtA

query
sites
2bhtA

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active site: K41 (= K45), S69 (= S73), Q199 (= Q203), G203 (= G207), S255 (= S259), C280 (= C284)
binding pyridoxal-5'-phosphate: K41 (= K45), N71 (= N75), M173 (= M177), G174 (= G178), T175 (= T179), T176 (= T180), T178 (= T182), G208 (= G212), S255 (= S259), C280 (= C284)

P47998 Cysteine synthase 1; At.OAS.5-8; Beta-substituted Ala synthase 1;1; ARAth-Bsas1;1; CSase A; AtCS-A; Cys-3A; O-acetylserine (thiol)-lyase 1; OAS-TL A; O-acetylserine sulfhydrylase; Protein ONSET OF LEAF DEATH 3; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
43% identity, 97% coverage: 8:298/300 of query aligns to 9:310/322 of P47998

query
sites
P47998

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K46 (= K45) modified: N6-(pyridoxal phosphate)lysine; mutation to A: No cysteine synthase activity.
T74 (= T72) mutation to A: Strong reduction of cysteine synthase activity.; mutation to S: Reduction of cysteine synthase activity.
S75 (= S73) mutation S->A,N,T: Strong reduction of cysteine synthase activity.
N77 (= N75) binding pyridoxal 5'-phosphate; mutation to A: Reduction of cysteine synthase activity.; mutation to D: Strong reduction of cysteine synthase activity.
T78 (= T76) mutation T->A,S: Reduction of cysteine synthase activity.
Q147 (= Q144) mutation Q->A,E: Strong reduction of cysteine synthase activity.
H157 (= H154) mutation H->Q,N: Slight reduction of cysteine synthase activity.
G162 (= G159) mutation to E: In old3-1; displays a early leaf death phenotype. Abolishes cysteine synthase activity.
GTGGT 181:185 (≠ GTTGT 178:182) binding pyridoxal 5'-phosphate
T182 (= T179) mutation T->A,S: Slight reduction of cysteine synthase activity.
T185 (= T182) mutation T->A,S: Strong reduction of cysteine synthase activity.
K217 (≠ R214) mutation to A: Impaired interaction with SAT1.
H221 (≠ Q218) mutation to A: Impaired interaction with SAT1.
K222 (≠ E219) mutation to A: Impaired interaction with SAT1.
S269 (= S259) binding pyridoxal 5'-phosphate; mutation to A: Strong reduction of cysteine synthase activity.; mutation to T: Reduction of cysteine synthase activity.

2isqA Crystal structure of o-acetylserine sulfhydrylase from arabidopsis thaliana in complex with c-terminal peptide from arabidopsis serine acetyltransferase (see paper)
43% identity, 97% coverage: 8:298/300 of query aligns to 7:308/320 of 2isqA

query
sites
2isqA

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A

active site: K44 (= K45), S267 (= S259)
binding pyridoxal-5'-phosphate: K44 (= K45), N75 (= N75), G177 (≠ S176), G179 (= G178), T180 (= T179), G181 (≠ T180), T183 (= T182), G223 (vs. gap), S267 (= S259), P294 (≠ C284)
binding : T72 (= T72), S73 (= S73), G74 (= G74), T76 (= T76), G122 (= G122), M123 (= M123), K124 (≠ E124), G217 (≠ W216), P218 (= P217), H219 (≠ Q218), Q222 (vs. gap), G223 (vs. gap)

1z7yA Crystal structure of the arabidopsis thaliana o-acetylserine sulfhydrylase k46a mutant (see paper)
43% identity, 97% coverage: 8:298/300 of query aligns to 7:308/320 of 1z7yA

query
sites
1z7yA

I

V

A

T

D

E

C

L

V

I

G

G

N

N

T

T

P

P

L

L

V

V

R

Y

L

L

Q

N

R

N

L

V

P

A

G

E

A

G

T

C

S

V

N

G

T

R

L

V

L

A

K

K

L

L

E

E

G

M

N

M

N

E

P

P

A

C

G

S

S

S

V

V

K
x
A

D

D

R

R

P

I

A

G

L

F

S

S

M

M

I

I

T

S

R

D

A

A

E

E

L

K

R

K

G

G

Q

L

I

I

H

K

A

P

G

G

D

E

T

S

-

V

L

L

I

I

E

E

A

P

T

T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

M

F

A

T

A

A

I

A

K

K

G

G

Y

Y

K

K

M

L

I

I

L

I

I

T

M

M

P

P

D

A

N

S

S

M

S

S

A

T

E

E

R

R

K

R

A

I

M

L

T

L

A

A

Y

F

G

G

A

V

E

E

L

L

I

V

L

L

V

T

S

D

Q

P

E

A

E

K

G

G

M

M

E

K

G

G

A

A

R

I

D

A

L

K

A

A

E

E

R

E

M

I

Q

L

A

A

E

K

G

T

-

P

R

N

G

G

K

Y

V

M

L

L

D

Q

Q
|
Q

F

F

A

E

N

N

G

P

D

A

N

N

P

P

E

K

A

I

H

H

Y

Y

T

E

G

T

T

T

G

G

P

P

E

E

I

I

W

W

R

K

Q

G

T

T

Q

G

G

G

T

K

I

I

T

D

H

G

F

F

I

V

S

S

S

G

M
x
I

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

M

T

G

G

V

A

S

G

R

K

Y

Y

L

L

K

K

E

E

Q

Q

N

N

P

A

A

N

V

V

Q

K

I

L

V

Y

G

G

L

V

Q

E

P

P

M

V

E

E

G

S

S

A

A

I

I

L

P

S

G

G

I

G

R

K

R

P

W

G

P

P

Q

H

E

K

-

I

-

Q

-
x
G

-

I

-

G

-

A

-

G

Y

F

L

I

P

P

K

S

I

V

Y

L

Q

N

A

V

D

D

R

L

V

I

D

D

R

E

I

V

V

V

D

Q

M

V

A

S

Q

S

S

D

E

E

A

S

E

I

D

D

V

M

T

A

R

R

R

Q

L

L

A

A

R

L

E

K

E

E

G

G

I

L

F

L

C

V

G

G

V

I

S
|
S

S

S

G

G

G

A

A

A

V

A

A

A

M

A

L

I

R

K

L

L

S

A

K

Q

E

R

V

P

E

E

N

N

A

A

-

G

-

K

V

L

M

F

V

V

A

A

I

I

I

F

C
x
P

D
x
S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

V

I

L

F

F

D

D

A

A

active site: A44 (≠ K45), S267 (= S259)
binding n-[(3-hydroxy-2-methyl-5-{[(trihydroxyphosphoranyl)oxy]methyl}pyridin-4-yl)methylene]methionine: G74 (= G74), N75 (= N75), T76 (= T76), Q145 (= Q144), I178 (≠ M177), G179 (= G178), T180 (= T179), G181 (≠ T180), T183 (= T182), G223 (vs. gap), S267 (= S259), P294 (≠ C284), S295 (≠ D285)

8b9wA Cysteine synthase from trypanosoma theileri with plp bound (see paper)
41% identity, 97% coverage: 6:296/300 of query aligns to 11:311/329 of 8b9wA

query
sites
8b9wA

P

P

T

S

I

V

A

D

D

A

C

L

V

I

G

G

N

N

T

T

P

P

L

A

V

V

R

Y

L

L

Q

R

R

N

L

M

P

-

G

N

A

K

T

T

S

A

N

A

T

T

L

I

L

V

L

L

K

K

L

L

E

E

G

S

N

E

N

N

P

P

A

M

G

A

S

S

V

V

K
|
K

D

D

R

R

P

L

A

A

L

Y

S

A

M

I

I

Y

T

D

R

K

A

A

E

E

L

K

R

E

G

G

Q

K

I

I

H

K

A

R

G

G

D

K

T

S

L

V

I

I

-

V

E

E

A

A

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

M

L

A

L

A

G

A

A

I

V

K

R

G

G

Y

Y

K

K

M

V

I

I

L

I

I

T

M

M

P

P

D

D

N

S

S

M

S

S

A

M

E

E

R

R

K

R

A

S

A

L

M

L

T

R

A

I

Y

F

G

G

A

A

E

E

L

L

I

I

L

L

V

T

S

P

Q

A

E

S

E

L

G

G

M

M

E

K

G

G

A

A

R

V

D

A

L

M

A

A

E

N

R

R

M

I

Q

V

A

S

E

N

G

N

R

P

G

D

K

A

V

V

L

L

-

A

D

D

Q

Q

F

F

A

C

N

T

G

K

D

Y

N

N

P

A

E

Q

A

I

H

H

Y

E

T

E

G

T

T

T

G

G

P

P

E

E

I

I

W

W

R

R

Q

Q

T

T

Q

K

G

G

T

H

I

V

T

D

H

C

F

F

I

V

S

A

S

G

M

V

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

L

M

T

G

G

V

V

S

A

R

R

Y

F

L

L

K

K

E

S

Q

V

N

G

P

C

A

N

V

A

Q

N

I

I

V

V

G

A

L

V

Q

E

P

P

M

Q

E

E

G

S

S

P

A

V

I

L

P

S

G

G

I

G

R

R

R

P

W

G

P

P

Q

H

E

R

-

I

-

Q

-
x
G

-

I

-

G

-

A

-

G

Y

F

L

V

P

P

K

E

I

V

Y

L

Q

D

A

R

D

E

R

L

V

V

D

D

R

E

I

I

V

I

D

Q

M

V

A

S

Q

S

S

D

E

K

A

A

E

I

D

E

V

T

T

A

R

Q

R

K

L

L

A

S

R

R

E

M

E

D

G

G

I

I

F

F

C

C

G

G

V

F

S
|
S

S

A

G

G

G

A

A

N

V

V

A

F

A

A

M

A

L

L

R

K

L

I

S

A

K

E

E

R

V

P

E

E

N

M

A

A

-

G

-

K

V

T

M

I

V

V

A

T

I

V

I

I

C
x
P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

A

I

L

F

Y

binding pyridoxal-5'-phosphate: K49 (= K45), N80 (= N75), G184 (= G178), T185 (= T179), G186 (≠ T180), T188 (= T182), G228 (vs. gap), S272 (= S259), P299 (≠ C284)

7n2tA O-acetylserine sulfhydrylase from citrullus vulgaris in the internal aldimine state, with citrate bound (see paper)
41% identity, 97% coverage: 8:298/300 of query aligns to 7:308/309 of 7n2tA

query
sites
7n2tA

I

V

A

T

D

E

C

L

V

I

G

G

N

N

T

T

P

P

L

L

V

V

R

Y

L

L

Q

N

R

R

L

V

P

V

G

D

A

G

T

C

S

V

N

A

T

R

L

V

L

A

K

K

L

L

E

E

G

M

N

M

N

E

P

P

A

C

G

S

S

S

V

V

K

K

D

D

R

R

P

I

A

G

L

Y

S

S

M

M

I

I

T

S

R

D

A

A

E

E

L

N

R

K

G

G

Q

L

I

I

H

T

A

P

G

G

D

E

T

S

-

V

L

L

I

I

E

E

A

P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

G

L

L

A

A

M

F

A

I

A

A

I

A

K

K

G

G

Y

Y

K

R

M

L

I

I

L

I

I

C

M

M

P

P

D

A

N

S

S

M

S

S

A

L

E

E

R

R

K

R

A

T

A

I

M

L

T

R

A

A

Y

F

G

G

A

A

E

E

L

L

I

V

L

L

V

T

S

D

Q

P

E

A

E

R

G

G

M

M

E

K

G

G

A

A

R

V

D

Q

L

K

A

A

E

E

R

E

M

I

Q

K

A

A

E

K

G

T

-

P

R

N

G

S

K

Y

V

I

L

L

D

Q

Q

Q

F

F

A

E

N

N

G

P

D

A

N

N

P

P

E

K

A

I

H

H

Y

Y

T

E

G

T

T

T

G

G

P

P

E

E

I

I

W

W

R

R

Q

G

T

S

Q

G

G

G

T

K

I

I

T

D

H

A

F

L

I

V

S

S

S

G

M

I

G

G

T

T

T

G

G

G

T

T

I

V

M

T

G

G

V

A

S

G

R

K

Y

Y

L

L

K

K

E

E

Q

Q

N

N

P

P

A

N

V

I

Q

K

I

L

V

Y

G

G

L

V

Q

E

P

P

M

V

E

E

G

S

S

A

A

I

I

L

P

S

G

G

I

G

R

K

R

P

W

G

P

P

Q

H

E

K

-

I

-

Q

-

G

-

I

-

G

-

A

-

G

Y

F

L

I

P

P

K

G

I

V

Y

L

Q

D

A

V

D

N

R

L

V

L

D

D

R

E

I

V

V

I

D

Q

M

V

A

S

Q

S

S

E

E

E

A

S

E

I

D

E

V

T

T

A

R

K

R

L

L

L

A

A

R

L

E

K

E

E

G

G

I

L

F

L

C

V

G

G

V

I

S

S

G

G

G

A

A

A

V

A

A

A

M

A

L

I

R
|
R

L

I

S

A

K

K

E
x
R

V

P

E

E

N

N

A

A

-

G

-

K

V

L

M

I

V

V

A

A

I

V

I

F

C

P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

V

I

L

F

F

D

E

A

S

binding phosphate ion: R277 (= R269), R281 (≠ E273)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
41% identity, 96% coverage: 8:296/300 of query aligns to 7:305/310 of P9WP55

query
sites
P9WP55

I

I

A

T

D

Q

C

L

V

I

G

G

N

R

T

T

P

P

L

L

V

V

R

R

L

L

Q

R

R

R

L

V

P

T

G

D

A

G

T

A

S

V

N

A

T

D

L

I

L

V

L

A

K

K

L

L

E

E

G

F

N

F

N

N

P

P

A

A

G

N

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

V

S

A

M

M

I

L

T

Q

R

A

A

A

E

E

L

Q

R

A

G

G

Q

L

I

I

H

K

A

P

G

D

D

T

T

I

L

I

I

L

E

E

A

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

M

M

A

V

A

C

A

A

I

A

K

R

G

G

Y

Y

K

R

M

C

I

V

L

L

I

T

M

M

P

P

D

E

N

T

S

M

S

S

A

L

E

E

R

R

K

R

A

M

A

L

M

L

T

R

A

A

Y

Y

G

G

A

A

E

E

L

L

I

I

L

L

V

T

S

P

Q

G

E

A

E

D

G

G

M

M

E

S

G

G

A

A

R

I

D

A

L

K

A

A

E

E

R

E

M

L

-

A

Q

K

A

T

E

D

G

Q

R

R

G

Y

K

F

V

V

L

P

D

Q

Q

Q

F

F

A

E

N

N

G

P

D

A

N

N

P

P

E

A

A

I

H

H

Y

R

T

V

G

T

T

T

G

A

P

E

E

E

I

V

W

W

R

R

Q

D

T

T

Q

D

G

G

T

K

I

V

T

D

H

I

F

V

I

V

S

A

S

G

M

V

G
|
G

T
|
T

T
x
G

G
|
G

T
|
T

I

I

M

T

G

G

V

V

S

A

R

Q

Y

V

L

I

K

K

E

E

Q

R

N

K

P

P

A

S

V

A

Q

R

I

F

V

V

G

A

L

V

Q

E

P

P

M

A

E

A

G

S

S

P

A

V

I

L

P

S

G

G

I

G

R

Q

R

K

W

G

P

P

Q

H

E

P

-

I

-

Q

-

G

-

I

-

G

-

A

-

G

Y

F

L

V

P

P

K

P

I

V

Y

L

Q

D

A

Q

D

D

R

L

V

V

D

D

R

E

I

I

V

I

D

T

M

V

A

G

Q

N

S

E

E

D

A

A

E

L

D

N

V

V

T

A

R

R

L

L

A

A

R

R

E

E

G

G

I

L

F

L

C

V

G

G

V

I

S
|
S

S

S

G

G

G

A

A

A

V

T

A

V

A

A

M

A

L

L

R

Q

L

V

S

A

K

R

E

R

V

P

E

E

N

N

A

A

-

G

-

K

V

L

M

I

V

V

A

V

I

V

I

L

C

P

D

D

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

P

I

L

F

F

K44 (= K45) modified: N6-(pyridoxal phosphate)lysine
N74 (= N75) binding pyridoxal 5'-phosphate
GTGGT 178:182 (≠ GTTGT 178:182) binding pyridoxal 5'-phosphate
S266 (= S259) binding pyridoxal 5'-phosphate

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
41% identity, 96% coverage: 8:296/300 of query aligns to 7:305/306 of 2q3dA

query
sites
2q3dA

I

I

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x
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active site: K44 (= K45), S266 (= S259), P293 (≠ C284)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K45), T71 (= T72), S72 (= S73), N74 (= N75), T75 (= T76), Q144 (= Q144), V177 (≠ M177), G178 (= G178), T179 (= T179), G180 (≠ T180), T182 (= T182), G222 (vs. gap), I223 (vs. gap), S266 (= S259), P293 (≠ C284), D294 (= D285)

Q93244 Cysteine synthase 1; O-acetylserine (thiol)-lyase 1; OAS-TL; EC 2.5.1.47 from Caenorhabditis elegans (see 2 papers)
41% identity, 96% coverage: 9:296/300 of query aligns to 12:310/341 of Q93244

query
sites
Q93244

A

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P75 (≠ A71) mutation to L: In n5537; severe loss of protein stability.
A88 (= A84) mutation to V: In n5522; severe loss of protein stability.
S144 (≠ K140) mutation to F: In mr26; susceptible to high levels of hydrogen sulfide.
G181 (≠ S176) mutation to E: In n5521 and mr23; severe loss of protein stability. Susceptible to high levels of hydrogen sulfide.
G183 (= G178) mutation to R: In n5515; severe loss of protein stability.
G229 (vs. gap) mutation to E: In mr33; susceptible to high levels of hydrogen sulfide.
R259 (= R247) mutation to K: In n5519; no loss of protein stability. No effect on enzyme activity.
S272 (= S260) mutation to F: In mr29; susceptible to high levels of hydrogen sulfide.
T295 (≠ I283) mutation to I: In mr39; susceptible to high levels of hydrogen sulfide.

8b9yC Cysteine synthase from trypanosoma cruzi with plp and oas (see paper)
40% identity, 98% coverage: 6:298/300 of query aligns to 12:314/330 of 8b9yC

query
sites
8b9yC

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S

binding pyridoxal-5'-phosphate: K50 (= K45), N81 (= N75), G185 (= G178), T186 (= T179), G187 (≠ T180), T189 (= T182), G229 (vs. gap), S273 (= S259), P300 (≠ C284), S301 (≠ D285)

8b9yA Cysteine synthase from trypanosoma cruzi with plp and oas (see paper)
41% identity, 98% coverage: 6:298/300 of query aligns to 10:297/313 of 8b9yA

query
sites
8b9yA

P

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F

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A

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K

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x
P

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x
S

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Y

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G

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Y

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A

S

binding o-acetylserine: T76 (= T72), G78 (= G74), N79 (= N75), T80 (= T76)
binding pyridoxal-5'-phosphate: V47 (= V44), K48 (= K45), N79 (= N75), G183 (= G178), T184 (= T179), G185 (≠ T180), T187 (= T182), S256 (= S259), P283 (≠ C284), S284 (≠ D285)
binding alpha-D-ribofuranose: C40 (≠ G37), E41 (≠ N38), N42 (= N39), P43 (= P40), A45 (≠ G42), Y285 (≠ R286)

4lmaA Crystal structure analysis of o-acetylserine sulfhydrylase cysk1 from microcystis aeruginosa 7806 (see paper)
41% identity, 96% coverage: 8:296/300 of query aligns to 7:308/318 of 4lmaA

query
sites
4lmaA

I

V

A

T

D

E

C

L

V

V

G

G

N

R

T

T

P

P

L

L

V

V

R

Q

L

L

Q

N

R

R

L

I

P

P

G

Q

A

A

T

E

S

G

-

C

-

L

N

G

T

R

L

I

L

V

L

M

K

K

L

L

E

E

G

G

N

M

N

N

P

P

A

A

G

A

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

T

S

H

M

M

I

I

T

N

R

S

A

A

E

E

L

K

R

A

G

G

Q

L

I

I

H

N

A

P

G

E

D

T

T

T

-

V

L

L

I

V

E

E

A

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

M

M

A

T

A

A

I

A

K

K

G

G

Y

Y

K

R

M

L

I

I

L

L

I

T

M

M

P

P

D

E

N

T

S

M

S

S

A

L

E

E

R

R

K

R

A

A

A

M

M

L

T

K

A

A

Y

Y

G

G

A

A

E

T

L

L

I

E

L

L

V

T

S

P

Q

G

E

S

E

Q

G

G

M

M

E

K

G

G

A

A

R

I

D

L

L

R

A

A

E

Q

R

Q

M

I

Q

V

A

D

E

S

G

I

R

P

G

G

K

A

-

Y

V

M

L

L

D

Q

Q

Q

F

F

A

R

N

N

G

P

D

S

N

N

P

P

E

E

A

I

H

H

Y

R

T

L

G

T

T

T

G

A

P

E

E

E

I

I

W

W

R

Q

Q

D

T

T

Q

E

G

G

T

Q

I

V

T

D

H

F

F

I

I

V

S

A

S

G

M

V

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

M

T

G

G

V

V

S

A

R

E

Y

V

L

I

K

K

E

S

Q

R

N

K

P

P

A

S

V

F

Q

Q

I

V

V

V

G

A

L

V

Q

E

P

P

M

F

E

N

G

S

S

P

A

V

I

I

P

S

G

G

I

G

R

N

R

P

W

G

P

P

Q

H

E

K

-

I

-

Q

-
x
G

-

I

-

G

-

A

-

G

Y

F

L

I

P

P

K

E

I

V

Y

L

Q

R

A

T

D

D

R

L

V

I

D

D

R

E

I

V

V

I

D

T

M

V

A

S

Q

D

S

E

E

E

A

A

E

F

D

Q

V

F

T

G

R

R

L

L

A

A

R

K

E

E

G

G

I

L

F

L

C

S

G

G

V

I

S
|
S

S

S

G

G

G

A

A

N

V

L

A

C

A

A

M

A

L

I

R

Q

L

L

S

A

K

Q

E

R

V

P

E

E

N

N

-

E

-

G

A

K

V

L

M

I

V

V

A

V

I

I

I

Q

C
x
P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

L

I

M

F

F

active site: K46 (= K45), S269 (= S259)
binding pyridoxal-5'-phosphate: K46 (= K45), N77 (= N75), G181 (= G178), T182 (= T179), G183 (≠ T180), T185 (= T182), G225 (vs. gap), S269 (= S259), P296 (≠ C284)

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
40% identity, 96% coverage: 9:297/300 of query aligns to 9:307/310 of 5xoqA

query
sites
5xoqA

A

S

D

E

C

S

V

I

G

G

N

R

T

T

P

P

L

L

V

V

R

Q

L

I

Q

N

R

R

L

L

P

T

G

A

A

G

T

L

S

S

N

S

T

R

L

V

L

L

L

A

K

K

L

I

E

E

G

G

N

R

N

N

P

P

A

A

G

Y

S

S

V

V

K

K

D

C

R

R

P

I

A

G

L

A

S

A

M

M

I

I

T

W

R

D

A

A

E

E

L

Q

R

S

G

G

Q

K

I

L

H

K

A

P

G

G

D

M

T

H

L

V

I

V

E

E

A

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

A

M

F

A

V

A

C

A

A

I

A

K

R

G

G

Y

Y

K

K

M

L

I

T

L

L

I

T

M

M

P

P

D

E

N

T

S

M

S

S

A

I

E

E

R

R

K

R

A

M

M

L

T

K

A

S

Y

F

G

G

A

A

E

D

L

L

I

V

L

L

V

T

S

P

Q

G

E

A

E

D

G

G

M
|
M

E

K

G

G

A

A

R

I

D

S

L

K

A

A

E

E

R

E

M

L

Q

A

A

A

E

Q

G

P

R

G

G

W

K

F

V

I

L

P

D

Q

Q
|
Q

F

F

A

K

N

N

G

P

D

A

N

N

P

P

E

A

A

I

H

H

Y

V

T

K

G

T

T

T

G

G

P

P

E

E

I

I

W

W

R

N

Q

D

T

T

Q

E

G

G

T

Q

I

V

T

D

H

V

F

F

I

V

S

A

S

G

M

V

G

G

T

T

T

G

G

G

T

T

I

I

M

T

G

G

V

V

S

A

R

R

Y

F

L

L

K

K

-

H

E

E

Q

K

N

K

P

H

A

P

V

V

Q

H

I

V

V

V

G

A

L

V

Q

E

P

P

M

A

E

A

G

S

S

P

A

V

I

L

P

A

G

G

-

G

-

P

-

A

-

G

-
x
R

-
x
H

-

K

I

I

R
x
Q

R
x
G

W

I

P

G

Q
x
A

E

G

Y

F

L

V

P

P

K

D

I

T

Y

F

Q

D

A

R

D

S

R

V

V

V

D

D

R

E

I

I

V

L

D

S

M

V

A

T

Q

D

S

D

E

E

A

A

E

I

D

E

V

T

T

A

R

R

R

K

L

L

A

A

R

M

E

E

G

G

I

I

F

S

C

C

G

G

V

I

S

S

S

C

G

G

G

A

A

A

V

M

A

A

A

G

M

A

L

L

R

K

L

V

S

A

K

A

E

R

V

P

E

E

N

F

A

A

-

G

-

K

V

T

M

I

V

V

A

T

I

V

I

L

C

P

D

D

R

A

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

A

I

L

F

F

D

E

binding : T72 (= T72), S73 (= S73), G74 (= G74), T76 (= T76), M123 (= M123), Q144 (= Q144), R218 (vs. gap), H219 (vs. gap), Q222 (≠ R214), G223 (≠ R215), A226 (≠ Q218)

3vbeC Crystal structure of beta-cyanoalanine synthase in soybean (see paper)
39% identity, 96% coverage: 8:296/300 of query aligns to 15:314/329 of 3vbeC

query
sites
3vbeC

I

V

A

S

D

Q

C

L

V

I

G

G

N

R

T

T

P

P

L

L

V

V

R

Y

L

L

Q

N

R

K

L

V

P

T

G

E

A

G

T

C

S

G

N

A

T

Y

L

V

L

A

L

V

K

K

L

Q

E

E

G

M

N

M

N

Q

P

P

A

T

G

A

S

S

V

I

K
|
K

D

D

R

R

P

P

A

A

L

Y

S

A

M

M

I

I

T

T

R

D

A

A

E

E

L

E

R

K

G

N

Q

L

I

I

H

T

A

P

G

G

D

K

T

T

-

T

L

L

I

I

E

E

A

P

T

T

S
|
S

G

G

N
|
N

T

M

G

G

I

I

A

S

L

M

A

A

M

F

A

M

A

A

I

M

K

K

G

G

Y

Y

K

K

M

M

I

V

L

L

I

T

M

M

P

P

D

S

N

Y

S

T

S

S

A

L

E

E

R

R

K

R

A

V

A

T

M

M

T

R

A

A

Y

F

G

G

A

A

E

E

L

L

I

I

L

L

V

T

S

D

Q

P

E

A

E

K

G

G

M

M

E

G

G

G

-

T

A

V

R

K

D

K

L

A

A

Y

E

E

R

L

M

L

Q

E

A

N

E

T

G

P

R

N

G

A

K

H

V

M

L

L

D

Q

Q

Q

F

F

A

S

N

N

G

P

D

A

N

N

P

T

E

Q

A

V

H

H

Y

F

T

E

G

T

T

T

G

G

P

P

E

E

I

I

W

W

R

E

Q

D

T

T

Q

N

G

G

T

Q

I

V

T

D

H

I

F

F

I

V

S
x
M

S

G

M

I

G
|
G

T
x
S

T
x
G

G

G

T
|
T

I

V

M

S

G

G

V

V

S

G

R

Q

Y

Y

L

L

K

K

E

S

Q

K

N

N

P

P

A

N

V

V

Q

K

I

I

V

Y

G

G

L

V

Q
x
E

P

P

M

S

E

E

G
x
S

S

N

A

V

I

L

P

N

G

G

I

G

R

K

R

P

W

G

P

P

Q

H

E

H

-

I

-

T

-
x
G

-

N

-

G

-

V

-

G

Y

F

L

K

P

P

K

D

I

I

Y

L