SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AO353_03310 FitnessBrowser__pseudo3_N2E3:AO353_03310 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P69451 Long-chain-fatty-acid--CoA ligase; Long-chain acyl-CoA synthetase; Acyl-CoA synthetase; EC 6.2.1.3 from Escherichia coli (strain K12) (see paper)
55% identity, 99% coverage: 2:562/566 of query aligns to 1:560/561 of P69451

query
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P69451

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Y213 (= Y214) mutation to A: Loss of activity.
T214 (= T215) mutation to A: 10% of wild-type activity.
G216 (= G217) mutation to A: Decreases activity.
T217 (= T218) mutation to A: Decreases activity.
G219 (= G220) mutation to A: Decreases activity.
K222 (= K223) mutation to A: Decreases activity.
E361 (= E362) mutation to A: Loss of activity.

6k4dA Ancestral luciferase anclamp in complex with atp and d-luciferin (see paper)
32% identity, 89% coverage: 48:552/566 of query aligns to 47:534/539 of 6k4dA

query
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binding [[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] (4S)-2-(6-oxidanyl-1,3-benzothiazol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylate: H243 (= H262), F245 (≠ Y264), T249 (≠ H269), G314 (= G335), A315 (≠ M336), P316 (≠ A337), G337 (= G357), Y338 (= Y358), G339 (= G359), L340 (≠ M360), T341 (= T361), S345 (≠ P365), A346 (≠ V366), D420 (= D439), I432 (= I451), K527 (= K545)
binding (4S)-2-(6-hydroxy-1,3-benzothiazol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid: F245 (≠ Y264), R335 (≠ C355), G337 (= G357), G339 (= G359), L340 (≠ M360), A346 (≠ V366)

6k4cA Ancestral luciferase anclamp in complex with dlsa (see paper)
32% identity, 89% coverage: 48:552/566 of query aligns to 47:534/538 of 6k4cA

query
sites
6k4cA

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|
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binding 5'-o-[n-(dehydroluciferyl)-sulfamoyl] adenosine: H243 (= H262), F245 (≠ Y264), T249 (≠ H269), G314 (= G335), A315 (≠ M336), P316 (≠ A337), G337 (= G357), Y338 (= Y358), G339 (= G359), L340 (≠ M360), T341 (= T361), A346 (≠ V366), D420 (= D439), I432 (= I451), K527 (= K545)

Q84P21 Peroxisomal OPC-8:0-CoA ligase 1; 4-coumarate--CoA ligase isoform 9; At4CL9; 4-coumarate--CoA ligase-like 5; EC 6.2.1.- from Arabidopsis thaliana (Mouse-ear cress) (see paper)
29% identity, 93% coverage: 24:551/566 of query aligns to 30:536/546 of Q84P21

query
sites
Q84P21

P

P

N

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I

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K530 (= K545) mutation to N: Lossed enzymatic activity.

4gxqA Crystal structure of atp bound rpmatb-bxbclm chimera b1 (see paper)
29% identity, 90% coverage: 47:553/566 of query aligns to 26:499/506 of 4gxqA

query
sites
4gxqA

G

G

K

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Y

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|
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D

P

E

D

R

G

G

Y

Y

M

V

R

H

I

I

V

L

D

G

R
|
R

K

G

K

K

D

D

M

L

I

V

L
x
I

V

T

S

G

G

G

F

F

N
|
N

V

V

Y

Y

P

P

N

K

E

E

L

I

E

E

D

S

V

E

L

I

A

D

T

A

L

M

P

P

G

G

V

V

L

V

Q

E

C

S

A

A

A

V

I

I

G

G

V

V

P

P

D

H

E

A

K

D

S

F

G

G

E

E

A

G

I

V

K

T

I

A

F

F

I

V

V

V

A

L

K

K

-

R

-

E

-

F

-

A

P

P

G

S

V

E

T

I

L

L

T

A

K

-

E

E

Q

E

V

L

M

K

T

A

H

F

M

V

R

K

A

D

N

R

V

L

T

A

G

K

Y

F

K

K

V

M

P

P

R

K

A

K

I

V

E

I

F

F

R

V

D

D

A

D

L

L

P

P

T

R

T

N

N

T

V

M

G

G

K
x
A

I

V

L

Q

R

K

R

N

E

V

L

L

R

R

D

E

active site: T163 (= T215), N183 (= N235), H207 (= H262), T303 (= T361), E304 (= E362), I403 (≠ L460), N408 (= N465), A491 (≠ K545)
binding adenosine-5'-triphosphate: T163 (= T215), S164 (≠ G216), G165 (= G217), T166 (= T218), T167 (= T219), H207 (= H262), S277 (≠ G335), A278 (≠ M336), P279 (≠ A337), E298 (= E356), M302 (= M360), T303 (= T361), D382 (= D439), R397 (= R454)
binding carbonate ion: H207 (= H262), S277 (≠ G335), R299 (≠ G357), G301 (= G359)

5bsrA Crystal structure of 4-coumarate:coa ligase complexed with adenosine monophosphate and coenzyme a (see paper)
30% identity, 94% coverage: 20:552/566 of query aligns to 9:525/528 of 5bsrA

query
sites
5bsrA

P

P

D

D

E

I

Y

Y

-

I

P

P

N

N

-

H

-

L

-

P

I

L

Q

H

A

S

V

Y

L

C

K

F

Q

E

S

N

C

I

Q

S

R

E

F

F

A

S

N

S

K

R

P

P

A

C

F

L

S

I

N

N

L

G

G

A

-

N

-

K

K

Q

T

I

L

Y

T

T

Y

Y

G

A

E

D

L

V

Y

E

E

L

L

N

S

S

G

R

A

K

F

V

A

A

Y

G

L

L

Q

H

Q

K

H

Q

T

-

D

G

L

I

Q

Q

P

P

G

K

D

D

R

T

I

I

A

M

V

I

Q

L

L

L

P

P

N

N

L

S

L

P

Q

E

Y

F

P

V

I

F

A

A

V

F

F

I

G

G

A

A

I

S

R

Y

A

L

G

G

L

A

I

I

V

S

V

T

N

M

T

A

N

N

P

P

L
|
L

Y

F

T

T

A

P

R

A

E

E

M

V

E

V

H

K

Q

Q

F

A

N

K

D

A

S

S

G

S

A

A

K

K

A

I

L

I

V

V

C

T

L

Q

A

A

N

C

M

H

A

V

H

N

L

K

A

V

E

K

A

D

V

Y

V

A

P

F

K

E

T

N

G

D

V

V

K

K

H

I

V

I

I

C

V

I

T

D

E

S

V

A

A

-

D

-

L

-

P

-

L

-

K

-

R

-

L

-

I

-

N

-

S

-

V

-

I

-

K

-

Y

-

V

-

K

-

M

-

V

-

P

-

A

-

Y

-

H

-

L

-

P

P

K

E

A

G

V

C

K

L

F

H

N

F

D

S

V

V

L

L

S

T

K

Q

G

A

H

N

G

E

Q

H

P

D

V

I

T

P

E

E

A

V

N

E

P

I

A

Q

S

P

S

D

D

D

V

V

A

V

V

A

L

L

Q

P

Y

Y

T
x
S

G

S

G

G

T

T

G

G

V

L

A

P

K

K

G

G

A

V

M

M

L

L

T

T

H

H

R

K

N

G

L

L

V

V

A

T

N
x
S

M

V

M

A

Q

Q

C

-

R

-

A

Q

L

V

M

D

G

G

S

E

N

N

L

P

N

N

-

L

-

Y

-

I

E

H

G

S

C

E

E

D

I

V

L

M

I

L

T

C

P

V

L

L

P
|
P

L

L

Y

F

H
|
H

I

I

Y
|
Y

A

S

F

L

T

N

F

S

H

V

C

L

M

L

A

C

M

G

M

L

L

R

I

V

G

G

N

A

H

A

N

-

I

I

L

L

I

I

S

M

N

Q

P

K

R
x
F

D

D

L

I

P

V

A

S

M

F

V

L

K

E

E

L

L

I

S

Q

K

R

W

Y

K

K

F

V

S

T

G

I

F

G

V

P

G

F

L

V

N

P

T

P

L

I

F

V

V

L

A

A

L

I

C

A

N

K

N

S

E

P

A

M

F

V

R

D

K

D

L

Y

D

D

F

L

S

S

G

S

L

V

K

R

I

T

T

V

L

M

S

S

G

G

G
x
A

M

A

A

P

L

L

-

G

-

K

Q

E

L

L

A

E

A

D

A

T

E

V

R

R

W

A

K

K

A

-

V

F

T

P

G

N

C

A

A

K

I

L

C

G

E

Q

G

G

Y

Y

G
|
G

M

M

T
|
T

E
|
E

T

A

S

G

P

P

V

V

-

L

-

A

-

M

-

C

-

L

A

A

T

F

V

A

N

K

P

E

N

P

Q

F

N

E

I

I

Q

K

I

S

G

G

T

A

I

C

G

G

I

T

P

V

V

V

P

R

S

N

T

A

L

E

C

M

K

K

V

I

I

V

D

D

-

P

D

K

A

T

G

G

V

N

E

S

Q

L

P

P

L

R

G

N

E

Q

I

S

G

G

E

E

L

I

C

C

V

I

K

R

G

G

P

D

Q

Q

V

I

M

M

K

K

G

G

Y

Y

W

L

Q

N

R

D

Q

P

E

E

A

A

T

T

D

A

E

R

I

T

L

I

D

D

S

K

E

E

G

G

W

W

L

L

K

Y

T

T

G

G

D
|
D

I

I

A

G

L

Y

I

I

Q

D

P

D

D

D

G

D

Y

E

M

L

R

F

I

I

V

V

D

D

R

R

K

L

K
|
K

D

E

M

L

I

I

L
x
K

V

Y

S
x
K

G
|
G

F
|
F

N
x
Q

V

V

Y

A

P

P

N

A

E

E

L

L

E

E

D

A

V

L

L

L

A

L

T

N

L

H

P

P

G

N

V

I

L

S

Q

D

C

A

A

A

A

V

I

V

G

P

V

M

P

K

D

D

E

E

K

Q

S

A

G

G

E

E

A

V

I

P

K

V

I

A

F

F

I

V

V

V

A

R

K

S

P

N

G

G

V

S

T

T

L

I

T

T

K

E

E

D

Q

E

V

V

M

K

T

D

H

F

M

I

R

S

A

K

N

Q

V

V

T

I

G
x
F

Y

Y

K

K

V

R

P

I

R

K

A

R

I

V

E

F

F

F

R

V

D

D

A

A

L

I

P

P

T

K

T

S

N

P

V

S

G

G

K
|
K

I

I

L

L

R

R

R

K

E

D

L

L

R

R

active site: S181 (≠ T215), S201 (≠ N235), H229 (= H262), T328 (= T361), E329 (= E362), K433 (≠ L460), Q438 (≠ N465), K518 (= K545)
binding adenosine monophosphate: A301 (≠ G335), G326 (= G359), T328 (= T361), D412 (= D439), K429 (= K456), K433 (≠ L460), Q438 (≠ N465)
binding coenzyme a: L102 (= L108), P226 (= P259), H229 (= H262), Y231 (= Y264), F253 (≠ R287), K435 (≠ S462), G436 (= G463), F437 (= F464), F498 (≠ G525)

O24146 4-coumarate--CoA ligase 2; 4CL 2; Nt4CL-19; Nt4CL-2; 4-coumaroyl-CoA synthase 2; Caffeate--CoA ligase; Cinnamate--CoA ligase; Ferulate--CoA ligase; EC 6.2.1.12; EC 6.2.1.-; EC 6.2.1.-; EC 6.2.1.34 from Nicotiana tabacum (Common tobacco) (see paper)
30% identity, 94% coverage: 20:552/566 of query aligns to 17:533/542 of O24146

query
sites
O24146

P

P

D

D

E

I

Y

Y

-

I

P

P

N

N

-

H

-

L

-

P

I

L

Q

H

A

S

V

Y

L

C

K

F

Q

E

S

N

C

I

Q

S

R

E

F

F

A

S

N

S

K

R

P

P

A

C

F

L

S

I

N

N

L

G

G

A

-

N

-

K

K

Q

T

I

L

Y

T

T

Y

Y

G

A

E

D

L

V

Y

E

E

L

L

N

S

S

G

R

A

K

F

V

A

A

Y

G

L

L

Q

H

Q

K

H

Q

T

-

D

G

L

I

Q

Q

P

P

G

K

D

D

R

T

I

I

A

M

V

I

Q

L

L

L

P

P

N

N

L

S

L

P

Q

E

Y

F

P

V

I

F

A

A

V

F

F

I

G

G

A

A

I

S

R

Y

A

L

G

G

L

A

I

I

V

S

V

T

N

M

T

A

N

N

P

P

L

L

Y

F

T

T

A

P

R

A

E

E

M

V

E

V

H

K

Q

Q

F

A

N

K

D

A

S

S

G

S

A

A

K

K

A

I

L

I

V

V

C

T

L

Q

A

A

N

C

M

H

A

V

H

N

L

K

A

V

E

K

A

D

V

Y

V

A

P

F

K

E

T

N

G

D

V

V

K

K

H

I

V

I

I

C

V

I

T

D

E

S

V

A

A

-

D

-

L

-

P

-

L

-

K

-

R

-

L

-

I

-

N

-

S

-

V

-

I

-

K

-

Y

-

V

-

K

-

M

-

V

-

P

-

A

-

Y

-

H

-

L

-

P

P

K

E

A

G

V

C

K

L

F

H

N

F

D

S

V

V

L

L

S

T

K

Q

G

A

H

N

G

E

Q

H

P

D

V

I

T

P

E

E

A

V

N

E

P

I

A

Q

S

P

S

D

D

D

V

V

A

V

V

A

L

L

Q

P

Y

Y

T
x
S

G
x
S

G
|
G

T
|
T

G

G

V

L

A

P

K
|
K

G

G

A

V

M

M

L

L

T

T

H

H

R

K

N

G

L

L

V

V

A

T

N

S

M

V

M

A

Q

Q

C

-

R

-

A

Q

L

V

M

D

G

G

S

E

N

N

L

P

N

N

-

L

-

Y

-

I

E

H

G

S

C

E

E

D

I

V

L

M

I

L

T

C

P

V

L

L

P

P

L

L

Y

F

H
|
H

I

I

Y
|
Y

A

S

F

L

T

N

F
x
S

H

V

C

L

M

L

A

C

M

G

M

L

L

R

I

V

G

G

N

A

H

A

N

-

I

I

L

L

I

I

S

M

N

Q

P
x
K

R

F

D

D

L

I

P

V

A

S

M

F

V

L

K

E

E

L

L

I

S

Q

K

R

W

Y

K

K

F

V

S

T

G

I

F

G

V

P

G

F

L

V

N

P

T

P

L

I

F

V

V

L

A

A

L

I

C

A

N

K

N

S

E

P

A

M

F

V

R

D

K

D

L

Y

D

D

F

L

S

S

G

S

L

V

K

R

I

T

T

V

L

M

S

S

G

G

G
x
A

M

A

A

P

L

L

-

G

-

K

Q

E

L

L

A

E

A

D

A

T

E

V

R

R

W

A

K

K

A

-

V

F

T

P

G

N

C

A

A

K

I

L

C

G

E
x
Q

G
|
G

Y

Y

G

G

M

M

T
|
T

E

E

T

A

S

G

P

P

V
|
V

-

L

-

A

-
x
M

-

C

-

L

A

A

T

F

V

A

N

K

P

E

N

P

Q

F

N

E

I

I

Q

K

I

S

G

G

T

A

I

C

G

G

I

T

P

V

V

V

P

R

S

N

T

A

L

E

C

M

K

K

V

I

I

V

D

D

-

P

D

K

A

T

G

G

V

N

E

S

Q

L

P

P

L

R

G

N

E

Q

I

S

G

G

E

E

L

I

C

C

V

I

K

R

G

G

P

D

Q

Q

V

I

M

M

K

K

G

G

Y

Y

W

L

Q

N

R

D

Q

P

E

E

A

A

T

T

D

A

E

R

I

T

L

I

D

D

S

K

E

E

G

G

W

W

L

L

K

Y

T

T

G

G

D
|
D

I

I

A

G

L

Y

I

I

Q

D

P

D

D

D

G

D

Y

E

M

L

R

F

I

I

V

V

D

D

R
|
R

K

L

K
|
K

D

E

M

L

I

I

L
x
K

V

Y

S
x
K

G
|
G

F

F

N
x
Q

V

V

Y

A

P

P

N

A

E

E

L

L

E

E

D

A

V

L

L

L

A

L

T

N

L

H

P

P

G

N

V

I

L

S

Q

D

C

A

A

A

A

V

I

V

G

P

V

M

P

K

D

D

E

E

K

Q

S

A

G

G

E

E

A

V

I

P

K

V

I

A

F

F

I

V

V

V

A

R

K

S

P

N

G

G

V

S

T

T

L

I

T

T

K

E

E

D

Q

E

V

V

M

K

T

D

H

F

M

I

R

S

A

K

N

Q

V

V

T

I

G

F

Y

Y

K

K

V

R

P

I

R

K

A

R

I

V

E

F

F

F

R

V

D

D

A

A

L

I

P

P

T

K

T

S

N

P

V

S

G

G

K
|
K

I

I

L

L

R

R

R

K

E

D

L

L

R

R

S189 (≠ T215) binding
S190 (≠ G216) binding
G191 (= G217) binding
T192 (= T218) binding
T193 (= T219) binding ; mutation to A: Reduced activity against 4-coumarate.
K197 (= K223) binding ; mutation to A: Reduced activity against 4-coumarate.
H237 (= H262) mutation to A: Strongly reduced activity against 4-coumarate.
Y239 (= Y264) binding ; binding ; binding ; mutation to A: Strongly reduced activity against 4-coumarate.; mutation to F: Reduced activity against 4-coumarate.
S243 (≠ F268) binding ; binding ; binding
K260 (≠ P286) binding
A309 (≠ G335) binding ; binding ; binding
Q331 (≠ E356) binding
G332 (= G357) binding ; binding ; binding ; binding ; binding
T336 (= T361) binding ; binding ; binding ; binding ; binding ; mutation to A: Strongly reduced activity against 4-coumarate.
V341 (= V366) mutation to G: Reduced activity against 4-coumarate.; mutation Missing: Reduced activity against 4-coumarate, but acquired ability to use sinapate as substrate.
M344 (vs. gap) binding ; binding ; binding ; mutation to A: Reduced activity against 4-coumarate.
D420 (= D439) binding ; binding ; binding ; binding ; binding
R435 (= R454) binding ; binding ; binding ; binding ; mutation to A: Strongly reduced activity against 4-coumarate.
K437 (= K456) binding ; binding ; binding ; binding
K441 (≠ L460) binding ; binding ; binding ; binding ; mutation to A: Abolished activity against 4-coumarate.
K443 (≠ S462) binding ; mutation to A: Normal activity against 4-coumarate.
G444 (= G463) binding
Q446 (≠ N465) binding
K526 (= K545) binding ; mutation to A: Abolished activity against 4-coumarate.

5bsvA Crystal structure of 4-coumarate:coa ligase complexed with feruloyl adenylate (see paper)
30% identity, 94% coverage: 20:552/566 of query aligns to 10:526/529 of 5bsvA

query
sites
5bsvA

P

P

D

D

E

I

Y

Y

-

I

P

P

N

N

-

H

-

L

-

P

I

L

Q

H

A

S

V

Y

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C

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F

A

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S

K

R

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P

A

C

F

L

S

I

N

N

L

G

G

A

-

N

-

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K

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T

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Y

T

T

Y

Y

G

A

E

D

L

V

Y

E

E

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G

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A

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A

A

Y

G

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L

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G

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D

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N

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E

Y

F

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F

A

A

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F

I

G

G

A

A

I

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R

Y

A

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G

G

L

A

I

I

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T

N

M

T

A

N

N

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P

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L

Y

F

T

T

A

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A

E

E

M

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E

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H

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A

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D

A

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G

S

A

A

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K

A

I

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I

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A

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x
S

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G

G

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G

G

A

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M

M

L

L

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H

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N

G

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x
S

M

V

M

A

Q

Q

C

-

R

-

A

Q

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D

G

G

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N

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G

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x
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x
A

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x
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-

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K

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E

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x
K

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G

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F

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x
Q

V

V

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A

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P

N

A

E

E

L

L

E

E

D

A

V

L

L

L

A

L

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N

L

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P

G

N

V

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Q

D

C

A

A

A

A

V

I

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P

K

D

D

E

E

K

Q

S

A

G

G

E

E

A

V

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P

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V

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A

F

F

I

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N

G

G

V

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E

E

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Q

E

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L

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R

active site: S182 (≠ T215), S202 (≠ N235), H230 (= H262), T329 (= T361), E330 (= E362), K434 (≠ L460), Q439 (≠ N465), K519 (= K545)
binding 5'-O-[(R)-hydroxy{[(2E)-3-(5-methoxy-4-oxocyclohexa-1,5-dien-1-yl)prop-2-enoyl]oxy}phosphoryl]adenosine: H230 (= H262), Y232 (= Y264), S236 (≠ F268), A302 (≠ G335), A303 (≠ M336), P304 (≠ A337), G325 (= G357), G327 (= G359), M328 (= M360), T329 (= T361), P333 (= P365), V334 (= V366), D413 (= D439), K430 (= K456), K434 (≠ L460), Q439 (≠ N465)

5bsuA Crystal structure of 4-coumarate:coa ligase complexed with caffeoyl adenylate (see paper)
30% identity, 94% coverage: 20:552/566 of query aligns to 10:526/529 of 5bsuA

query
sites
5bsuA

P

P

D

D

E

I

Y

Y

-

I

P

P

N

N

-

H

-

L

-

P

I

L

Q

H

A

S

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Y

L

C

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F

Q

E

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R

E

F

F

A

S

N

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K

R

P

P

A

C

F

L

S

I

N

N

L

G

G

A

-

N

-

K

K

Q

T

I

L

Y

T

T

Y

Y

G

A

E

D

L

V

Y

E

E

L

L

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S

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R

A

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F

V

A

A

Y

G

L

L

Q

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Q

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H

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G

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I

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Q

P

P

G

K

D

D

R

T

I

I

A

M

V

I

Q

L

L

L

P

P

N

N

L

S

L

P

Q

E

Y

F

P

V

I

F

A

A

V

F

F

I

G

G

A

A

I

S

R

Y

A

L

G

G

L

A

I

I

V

S

V

T

N

M

T

A

N

N

P

P

L

L

Y

F

T

T

A

P

R

A

E

E

M

V

E

V

H

K

Q

Q

F

A

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K

D

A

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G

S

A

A

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K

A

I

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A

A

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A

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H

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A

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E

K

A

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K

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A

A

-

D

-

L

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V

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I

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A

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D

D

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x
S

G

S

G

G

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G

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K