SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AO353_03845 FitnessBrowser__pseudo3_N2E3:AO353_03845 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 1 hits to proteins with known functional sites (download)

P68183 Maltose/maltodextrin transport system permease protein MalG from Escherichia coli (strain K12) (see 2 papers)
30% identity, 51% coverage: 85:229/282 of query aligns to 87:236/296 of P68183

query
sites
P68183

T

N

S

S

L

V

M

K

V

V

S

A

F

G

M

I

A

S

M

A

I

I

F

G

A

I

T

V

L

A

V

L

A

S

V

T

P

T

A

C

A

A

Y

Y

A

A

L

F

T

A

R

R

L

M

E

R

F

F

R

P

G

G

K

K

A

A

M

T

A

L

L

L

Q

K

L

G

M

M

S

L

L

I

P

F

L

Q

V

M

F

F

P

P

M

A

V

V

V

L

L

S

G

L

L

V

A

A

L

L

L

Y

L

A

V

L

F

F

D

D

S

R

L

L

-

G

-

E

-

Y

-

I

P

P

F

F

-

I

Q

G

M

L

T

N

V

T

S

H

R

G

L

G

V

V

I

I

A

F

H

A

V

Y

I

L

L

G

A

G

L

I

P

A

F

L

V

H

V

V

K

W

N

T

C

I

T

K

A

G

A

Y

M

F

L

E

S

T

I

I

G

D

S

S

E

S

V

L

E

E

E
|
E

A

A

A
|
A

Q

A

M

L

L

D

G
|
G

A

A

S

T

P

P

T

W

R

Q

A

A

I

F

V

R

D

L

V

V

V

L

V

L

P
|
P

L

L

M

S

K

V

S

P

G

I

I

L

L

A

A

V

G

V

M

F

L

I

L

L

A

S

F

F

I

I

V

A

S

A

F

I

N

T

E

E

F

V

T

P

V

V

T

A

Y

S

F

L

L

L

E190 (= E183) mutation E->A,C,K,L: Reduction of transport rate.
A192 (= A185) mutation A->D,S,L: Loss of transport and MalK dissociation from the membrane.
G196 (= G189) mutation to A: No effect.; mutation to P: Loss of transport and MalK dissociation from the membrane.
P209 (= P202) mutation to A: No effect.

Query Sequence

>AO353_03845 FitnessBrowser__pseudo3_N2E3:AO353_03845
MSTLSKKRLSLLPGDTGKFAGILSGVILFLAVLPILTMIVMSFSGSSNLDFPPSSYSLQW
YKAAWHTFVSPDASDVLSLGKAMSTSLMVSFMAMIFATLVAVPAAYALTRLEFRGKAMAL
QLMSLPLVFPMVVLGLALLLVFDSLPFQMTVSRLVIAHVILALPFVVKNCTAAMLSIGSE
VEEAAQMLGASPTRAIVDVVVPLMKSGILAGMLLAFIVSFNEFTVTYFLYTIDVMTVPIW
MFSRTVSSLDPTVFSFAVLIVLIDFVLIWALEKLVGEGGVSF

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory