SitesBLAST

HLN 66:68 (= HLN 69:71) binding glycerol
Y138 (≠ F141) binding glycerol
GFA 199:201 (= GFA 202:204) binding glycerol
N203 (= N206) binding glycerol
R206 (= R209) binding glycerol
PL 236:237 (≠ PI 239:240) mutation to FW: No detectable water or glycerol permeability.

1fx8A Crystal structure of the e. Coli glycerol facilitator (glpf) with substrate glycerol (see paper)
74% identity, 90% coverage: 9:262/283 of query aligns to 1:254/254 of 1fx8A

query
sites
1fx8A

S

T

L

L

S

K

S

G

Q

Q

C

C

M

I

A

A

E

E

F

F

L

L

G

G

T

T

A

G

L

L

I

I

F

F

G

G

T

V

G

G

C

C

V

V

A

A

L

L

K

K

V

V

A

A

G

G

A

A

S

S

F

F

G

G

L

Q

W

W

E

E

I

I

S

S

I

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I

I

W
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W

G

G

I

L

G

G

V

V

S

A

M

M

A

A

I

I

Y

Y

L

L

T

T

A

A

G

G

V

V

S

S

G

G

A

A

H

H

L

L

N
|
N

P

P

A

A

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V

S

T

I

I

A

A

L

L

C

W

I

L

F

F

A

A

D

C

F

F

E

D

K

K

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R

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K

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I

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P

Y

F

I

I

F

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S

Q

Q

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V

A

A

G

G

A

A

F

F

C

C

A

A

L

A

L

L

V

V

Y

Y

T

G

L

L

Y

Y

S

Y

N

N

L

L

F

F

D

D

F

F

E

E

Q

Q

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T

H

H

Q

H

M

I

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R

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E

D

L

L

A

A

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F

F

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Y

P

P

N

N

P

P

A

H

L

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N

T

F

A

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Q

A

A

F

F

L

A

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M

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D

N

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R

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G

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L

A

A

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P

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I

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G

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I

A

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M

M

G

G

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P

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T

G
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G

F
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A
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A

M

M

N
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N

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P

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A

R
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R

D

D

F

F

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G

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P

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K

L

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M

F

T

A

F

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F

L

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E

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T

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Y

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I

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F

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P

P

I

I

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G

A

A

C

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G

A

A

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H

H

L

L

binding glycerol: W43 (= W51), N63 (= N71), G194 (= G202), F195 (= F203), A196 (= A204), N198 (= N206), R201 (= R209)

B1VB61 Propanediol uptake facilitator PduF from Citrobacter freundii (see paper)
66% identity, 95% coverage: 6:273/283 of query aligns to 1:268/269 of B1VB61

query
sites
B1VB61

Q

M

Q

N

P

D

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A

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F

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T

A

G

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I

L

F

F

G

G

T

I

G

G

C

C

V

L

A

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A

A

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K

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V

A

A

G

G

A

A

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S

F

L

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G

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W

E

E

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C

I

I

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W

G

G

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G

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M

L

A

A

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Y

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L

T

T

A

A

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G

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S

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G

A

A

H

H

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L

N
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N

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A
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A

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A

A

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C

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I

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F

F

A

A

D

C

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Y

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A

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A

A

G

G

A

A

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F

C

G

A

G

A

A

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A

Y

Y

T

L

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Y

Y

S

G

N

S

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L

F

F

F

T

D

E

F

Y

E

E

Q

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T

A

H

H

Q

Q

M

M

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R

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E

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E

H

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A

A

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I

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T

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Y

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A

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A

W

Q

Q

A

A

F

A

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M

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G

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M

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A

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T

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D

D

N

G

N

N

G

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K

G

G

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P

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L

A

A

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P

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L

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I

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G

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A

A

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I

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G

S

A

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M

T

G

G

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P

L

L

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T

G

G

F

F

A

A

M

M

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A
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A

R

R

D

D

F

F

G

G

P

P

K

K

L

L

M

F

T

A

F

W

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A

A

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G

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D

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Y

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A

A

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G

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A

A

C

C

L

A

G

G

A

A

A

I

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R

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Y

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N

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C

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D

D

E

A

N

E

E

NPA 66:68 (= NPA 71:73) NPA 1
NPA 201:203 (= NPA 206:208) NPA 2

P37451 Propanediol uptake facilitator PduF from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
68% identity, 90% coverage: 9:263/283 of query aligns to 4:258/264 of P37451

query
sites
P37451

S

S

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L

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A

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Q

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A

A

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E

F

F

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G

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I

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C

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A

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A

A

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A

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C

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A

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T

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A

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A

Q

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A

A

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C

G

A

G

A

A

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S

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L

F

F

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D

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F

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E

Q

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A

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H

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M

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D

N

G

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N

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K

G

G

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L

A

A

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G

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G

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G

F

F

A

A

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M

N
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N

P
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A
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A

R

R

D

D

F

F

G

G

P

P

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K

L

L

M

F

T

T

F

W

F

L

A

A

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W

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E

N

I

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S

A

F

M

T

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E

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I

P

P

Y

Y

F

F

L

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A

A

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G

G

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A

C

C

L

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A

A

A

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H

N

L

L

P

P

NPA 66:68 (= NPA 71:73) NPA 1
NPA 201:203 (= NPA 206:208) NPA 2

Q96PS8 Aquaporin-10; AQP-10; Aquaglyceroporin-10; Small intestine aquaporin from Homo sapiens (Human) (see 2 papers)
40% identity, 95% coverage: 10:277/283 of query aligns to 20:277/301 of Q96PS8

query
sites
Q96PS8

L

L

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E

F

F

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A

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F

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A

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A

A

A

Q

L

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K

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A

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K

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N

W

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F

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W

A

G

G

-

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A

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T
x
G

A

G

G

N

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S

G

G

A

A

H
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H

L

L

N

N

P

P

A

A

V
x
F

S

S

I

L

A

A

L

M

C

C

I

I

F

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A

G

D
x
R

F

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W

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L

L

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P

L

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A

A

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C

A

A

A

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G

L

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Y

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L

L

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N

D

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D

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F

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T

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H
x
N

Q

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T

G

G

T

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Q

K

A

-

S

-

L

-

E

E

L

T

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A

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I

F

F

S

A

T

T

F

Y

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N

A

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A

Y

L

L

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S

T

L

A

N

Q

N

A

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Q

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I

-

V

G

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A

S

I

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T

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D

R

N

N

N

K

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A

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M

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A

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C

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N

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A

A

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R

D

D

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F

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A

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A

G

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D

G

I

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P

-

Y

W

F

W

L

W

I

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A

C

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G

L

L

L

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V

A

A

R

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H

H

L

H

P

P

I

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A

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I

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A

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K

E

D

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A

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A

A

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Q

D

D

E27 (= E17) mutation to Q: Loss of glycerol channel activity.
G73 (≠ T62) mutation to A: Increased glycerol channel activity at acidic pH.; mutation to F: Loss of glycerol channel activity.
S77 (= S66) mutation S->A,D: Decreased glycerol channel activity.
H80 (= H69) pH-sensor; mutation to A: Loss of glycerol channel activity.
F85 (≠ V74) mutation to A: Decreased glycerol channel activity.
R94 (≠ D83) mutation to A: Loss of glycerol channel activity.
N133 (≠ H122) modified: carbohydrate, N-linked (GlcNAc...) asparagine; mutation to Q: Abolishes N-glycosylation.

6f7hC Crystal structure of human aqp10 (see paper)
41% identity, 89% coverage: 10:261/283 of query aligns to 5:252/253 of 6f7hC

query
sites
6f7hC

L

L

S

A

S

R

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Q

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C

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L

A

A

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E

F

F

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A

A

A

Q

L

A

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G

G

A

E

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T

F

K

G

G

L

N

W

F

E

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M

I

F

I

L

W

A

G

G

-

S

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A

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V

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A

A

I
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I

Y

Y

L

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T

G

A

G

G

N

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S

G

G

A

A

H

H

L

L

N

N

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P

A

A

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F

S

S

I

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A

A

L

M

C

C

I

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K

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L

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L

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Y

I

I

F

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Q

V

L

A

L

G

S

A

A

F

F

C

C

A

A

A

S

L

G

L

A

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T

Y

Y

T

V

L

L

Y

Y

S

H

N

D

L

A

F

L

F

Q

D

N

F

Y

E

T

Q

G

T

G

H

N

Q

L

M

T

V

V

R

T

G

G

T

P

Q

K

A

-

S

-

L

-

E

E

L

T

A

A

S

S

V

I

F

F

S

A

T

T

F

Y

P

P

N

A

P

P

A

Y

L

L

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S

T

L

A

N

Q

N

A

G

F

F

L

L

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D

E

Q

V

V

I

L

I

G

T

T

A

G

I

M

L

L

M

I

-

V

G

G

V

L

I

L

M

A

S

I

L

L

T

D

D

R

N

N

N

K

G

G

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P

P

K

A

G

G

P

-

L

L

A

E

P

P

L

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V

G

G

L

M

L

L

I

I

A

L

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A

I

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G

G

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L

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S

M

M

G

G

P

A

L

N

T

C

G

G

F

I

A

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M

L

N
|
N

P

P

A

A

R

R

D

D

F

L

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G

P

P

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R

L

L

M

F

T

T

F

Y

F

V

A

A

G

G

W

W

G

G

E

P

I

E

S

V

F

F

T

S

G

A

G

G

R

N

D

G

I

-

P

-

Y

W

F

W

L

W

I

V

P

P

I

V

F

V

A

A

P

P

I

L

L

V

G

G

A

A

C

T

L

V

G

G

A

T

A

A

A

T

Y

Y

R

Q

G

L

L

L

I

V

A

A

R

L

H

H

binding glycerol: I55 (= I59), N199 (= N206)

O14520 Aquaporin-7; AQP-7; Aquaglyceroporin-7; Aquaporin adipose; AQPap; Aquaporin-7-like from Homo sapiens (Human) (see 5 papers)
34% identity, 91% coverage: 7:263/283 of query aligns to 30:281/342 of O14520

query
sites
O14520

Q

Q

P

R

S

K

L

M

S

V

S

R

Q

E

C

F

M

L

A

A

E

E

F

F

L

M

G

S

T

T

A

Y

L

V

L

M

I

M

F

V

F

F

G

G

T

L

G

G

C

S

V

V

A

A

A

H

L

M

-
x
V

-

L

-

N

K

K

V

K

A

Y

G

G

A

S

S
x
Y

F

L

G

G

L

-

W

-

E

-

I

V

S

N

I

L

I

G

W
x
F

G

G

I

F

G

G

V

V

S

T

M

M

A

G

I

V

Y

H

L

V

T

A

A

G

G

R

V

I

S

S

G

G

A

A

H

H

L

M

N

N

P

A

A

A

V

V

S

T

I

F

A

A

L

N

C

C

I

A

F

L

A

G

D

R

F

V

E

P

K

W

R

R

K

K

L

F

P

P

L

V

Y

Y

I

V

F

L

S

G

Q

Q

V

F

A

L

G

G

A

S

F

F

C

L

A

A

L

A

L

T

V

I

Y

Y

T

S

L

L

Y

F

S
x
Y

N

T

L

A

F

I

F

L

D

H

F

F

E

S

Q

G

T

G

H

Q

Q

L

M

M

V

V

R

T

G

G

T

P

Q

V

A

A

S

T

L

-

E

-

L

-

A

A

S

G

V

I

F

F

S

A

T

T

F

Y

P

L

N

P

P

D

A
x
H

L

M

S

T

T

L

A

W

Q

R

A

G

F

F

L

L

V

N

E

E

V

A

I

W

I

L

T

T

A

G

I

M

L

L

M

Q

G

L

V

C

I

L

M

F

S

A

L

I

T

T

D

D

D

Q

N

E

N

N

G

N

L

P

P

A

K

L

G

P

P

G

L

T

A

E

P

A

L

L

L

V

I

I

G

G

L

I

L

L

I

V

A

V

V

I

I

I

G

G

S

V

S

S

M

L

G

G

P

M

L

N

T

T

G

G

F
x
Y

A

A

M

I

N

N

P

P

A

S

R

R

D

D

F

L

G

P

P

P

K

R

L

I

M

F

T

T

F

F

F

I

A

A

G

G

W

W

G

G

E

K

I

Q

S

V

F

F

T

S

G

N

G

G

R

E

D

N

I

-

P

-

Y

W

F

W

L

W

I

V

P

P

I

V

F

V

A

A

P

P

I

L

L

L

G
|
G

A

A

C

Y

L

L

G

G

A

G

A

I

Y

Y

R

L

G

V

L

F

I

I

A

G

R

S

H

T

L

I

P

P

V59 (vs. gap) to L: in dbSNP:rs4008659
Y67 (≠ S41) mutation to A: No effect on glycerol channel activity. No effect on water channel activity.
F74 (≠ W51) mutation to W: No effect on glycerol channel activity. No effect on water channel activity. Decreased glycerol channel activity; when associated with F-233.
Y135 (≠ S112) Important for permeability to glycerol; mutation to A: Strongly decreased glycerol channel activity. Mildly decreased water channel activity.
H165 (≠ A145) mutation to A: Decreased glycerol channel activity. Mildly decreased water channel activity.
Y223 (≠ F203) mutation to F: No effect on glycerol channel activity. No effect on water channel activity. Decreased glycerol channel activity; when associated with W-74.
G264 (= G246) to V: loss of glycerol channel activity; loss of water channel activity; dbSNP:rs62542743

Sites not aligning to the query:

10:11 ST→AA: Loss of phosphorylation by PKA. Increased interaction with PLIN1.
12 R → C: in dbSNP:rs139297434

8c9hA Aqp7_inhibitor (see paper)
34% identity, 88% coverage: 7:254/283 of query aligns to 5:247/253 of 8c9hA

query
sites
8c9hA

Q

Q

P

R

S

K

L

M

S

V

S

R

Q

E

C

F

M

L

A

A

E

E

F

F

L

M

G

S

T

T

A

Y

L

V

L

M

I

M

F

V

F

F

G

G

T

L

G

G

C

S

V

V

A

A

A

H

L

M

-

V

-

L

-

N

K

K

V

K

A

Y

G

G

A

S

S

Y

F

L

G

G

L

-

W

-

E

-

I

V

S

N

I

L

I

G

W
x
F

G

G

I

F

G

G

V

V

S

T

M

M

A

G

I

V

Y

H

L

V

T

A

A

G

G

R

V

I

S

S

G

G

A
|
A

H
|
H

L

M

N

N

P

A

A

A

V

V

S

T

I

F

A

A

L

N

C

C

I

A

F

L

A

G

D

R

F

V

E

P

K

W

R

R

K

K

L

F

P

P

L

V

Y

Y

I

V

F

L

S

G

Q

Q

V

F

A

L

G

G

A

S

F

F

C

L

A

A

L

A

L

T

V

I

Y

Y

T

S

L

L

Y

F

S

Y

N

T

L

A

F

I

F

L

D

H

F

F

E

S

Q

G

T

G

H

Q

Q

L

M

M

V

V

R

T

G

G

T

P

Q

V

A

A

S

T

L

-

E

-

L

-

A

A

S

G

V

I

F

F

S

A

T

T

F

Y

P

L

N

P

P

D

A

H

L

M

S

T

T

L

A

W

Q

R

A

G

F

F

L

L

V

N

E

E

V

A

I

W

I

L

T

T

A

G

I

M

L
|
L

M
x
Q

G

L

V

C

I

L

M
x
F

S

A

L

I

T

T

D

D

D

Q

N

E

N

N

G

N

L

P

P

A

K

L

G

P

P

G

L

T

A

E

P

A

L

L

L

V

I
|
I

G

G

L

I

L

L

I

V

A

V

V

I

I

I

G

G

S

V

S

S

M

L

G

G

P

M

L

N

T

T

G

G

F

Y

A

A

M

I

N

N

P

P

A

S

R

R

D

D

F

L

G

P

P

P

K

R

L

I

M

F

T

T

F

F

F

I

A

A

G

G

W

W

G

G

E

K

I

Q

S

V

F

F

T

S

G

N

G

G

R

E

D

N

I

-

P

-

Y

W

F

W

L

W

I

V

P

P

I

V

F

V

A

A

P

P

I

L

L

L

G

G

A

A

C

Y

L

L

G

G

A

G

A

I

Y

Y

binding ethyl 4-[(4-pyrazol-1-ylphenyl)methylcarbamoylamino]benzoate: F49 (≠ W51), A66 (= A68), H67 (= H69), L157 (= L162), Q158 (≠ M163), F162 (≠ M167), I181 (= I186)

6n1gA Crystal structure of aquaglyceroporin aqp7 (see paper)
34% identity, 87% coverage: 9:254/283 of query aligns to 1:241/249 of 6n1gA

query
sites
6n1gA

S

K

L

M

S

V

S

R

Q

E

C

F

M

L

A

A

E

E

F

F

L

M

G

S

T

T

A

Y

L

V

L

M

I

M

F

V

F

F

G

G

T

L

G

G

C

S

V

V

A

A

A

H

L

M

-

V

-

L

-

N

K

K

V

K

A

Y

G

G

A

S

S

Y

F

L

G

G

L

-

W

-

E

-

I

V

S

N

I

L

I

G

W

F

G

G

I

F

G

G

V

V

S

T

M

M

A

G

I

V

Y

H

L

V

T

A

A

G

G

R

V

I

S

S

G

G

A

A

H

H

L

M

N

N

P

A

A

A

V

V

S

T

I

F

A

A

L

N

C

C

I

A

F
x
L

A

G

D
x
R

F

V

E

P

K

W

R

R

K

K

L

F

P

P

L

V

Y

Y

I

V

F

L

S

G

Q

Q

V

F

A

L

G

G

A

S

F

F

C

L

A

A

L

A

L

T

V

I

Y

Y

T

S

L

L

Y

F

S

Y

N

T

L

A

F

I

F

L

D

H

F

F

E

S

Q

G

T

G

H

Q

Q

L

M

M

V

V

R

T

G

G

T

P

Q

V

A

A

S

T

L

-

E

-

L

-

A

A

S

G

V

I

F

F

S

A

T

T

F

Y

P

L

N

P

P

D

A

H

L

M

S

T

T

L

A

W

Q

R

A

G

F

F

L

L

V

N

E

E

V

A

I

W

I

L

T

T

A

G

I

M

L

L

M

Q

G

L

V

C

I

L

M

F

S

A

L

I

T

T

D

D

D

Q

N

E

N

N

G

N

L

P

P

A

K

L

G

P

P

G

L

T

A

E

P

A

L

L

L

V

I

I

G

G

L

I

L

L

I

V

A

V

V

I

I

I

G

G

S

V

S

S

M

L

G

G

P

M

L

N

T

T

G

G

F

Y

A

A

M

I

N

N

P

P

A

S

R

R

D

D

F

L

G

P

P

P

K

R

L

I

M

F

T

T

F

F

F

I

A

A

G

G

W

W

G

G

E

K

I

Q

S

V

F

F

T

S

G

N

G

G

R

E

D

N

I

-

P

-

Y

W

F

W

L

W

I

V

P

P

I

V

F

V

A

A

P

P

I

L

L

L

G

G

A

A

C

Y

L

L

G

G

A

G

A

I

Y

Y

binding glycerol: L73 (≠ F81), R75 (≠ D83)

I1CR68 Aquaporin-1 from Rhizopus delemar (strain RA 99-880 / ATCC MYA-4621 / FGSC 9543 / NRRL 43880) (Mucormycosis agent) (Rhizopus arrhizus var. delemar) (see paper)
38% identity, 83% coverage: 15:250/283 of query aligns to 62:292/306 of I1CR68

query
sites
I1CR68

M

L

A

A

E

E

F

F

L

I

G

G

T

T

A

V

L

I

L

L

I

V

F

L

G

T

T

C

G

G

C

F

V

C

A

A

A

E

L

Q

K

T

V

L

A

H

G

I

A

E

S

E

F

S

G

K

L

S

W

W

E

L

I

T

S

S

I

S

I

F

W

G

-

S

G

G

I

L

G

S

V

V

S

L

M

I

A

G

I

I

Y

C

L

V

T

S

A

G

G

H

V

V

S

S

G

G

A

A

H

H

L

L

N

N

P

P

A

A

V

V

S

T

I

I

A

A

L

F

C

C

I

I

F

F

A

S

D

G

F

F

E

P

K

I

R

R

K

K

L

V

P

P

L

S

Y

Y

I

I

F

T

S

A

Q

Q

V

L

A

L

G

G

A

A

F

F

C

A

A

G

A

A

L

A

L

L

V

L

Y

Y

T

I

L

I

Y

I

S

E

N

P

L

A

F

I

F

V

D

Q

F

F

E

D

Q

G

T

G

H

Q

Q

R

M

Y

V

I

R

L

G

G

T

E

Q

K

A

S

S

T

L

-

E

-

L

-

A

A

S

G

V

I

F

F

S

G

T

T

F

Y

P

P

N

P

P

L

A

Y

L

V

S

G

T

I

A

G

Q

S

A

A

F

I

L

A

V

S

E

E

V

I

I

M

I

G

T

T

A

A

I

M

L

L

M

L

G

L

V

V

I

I

M

M

S

-

L

V

T

T

D

G

D

H

N

P

N

N

G

N

L

L

P

P

K

Y

G

K

P

S

L

A

A

Q

P

G

L

A

L

M

I

I

G

A

L

L

L

G

I

I

A

T

V

T

I

I

G

S

S

L

S

C

M

I

G

G

P

Y

L

T

T

S

G

G

F

F

A

S

M

L

N

N

P

P

A

A

R

R

D

D

F

F

G

G

P

P

K

R

L

L

M

F

T

T

F

A

F

I

A

A

G

G

W

W

G

G

E

F

I

D

S

V

F

F

T

K

G

V

G

Y

R
x
H

D

Y

I

-

P

-

Y

Y

F

A

L

L

I

V

P

P

I

M

F

F

A

A

P

P

I

I

L

L

G

G

A

G

C

L

L

V

G

G

H275 (≠ R231) mutation to A: Affects pH sensing; when associated with A-85.

Q8II36 Aquaglyceroporin; PfAQP; Aquaporin-1 from Plasmodium falciparum (isolate 3D7) (see 2 papers)
34% identity, 94% coverage: 8:273/283 of query aligns to 7:254/258 of Q8II36

query
sites
Q8II36

P

K

S

S

L

Y

S

V

S

R

Q

E

C

F

M

I

A

G

E

E

F

F

L

L

G

G

T

T

A

F

L

V

L

L

I

M

F

F

F

L

G

G

T

E

G

G

C

A

V

T

A

A

A

N

L

F

K

H

V

T

A

T

G

G

A

L

S

S

F

G

G

D

L

W

W

Y

E

K

I

L

S

C

I

L

I

G

W

W

G

G

I

L

G

A

V

V

S

F

M

F

A

G

I

I

Y

L

L

V

T

S

A

A

G

K

V

L

S

S

G
|
G

A
|
A

H

H

L

L

N
|
N

P
x
L

A
|
A

V

V

S

S

I

I

A

G

L

L

C

S

I

S

F

I

A

N

D

K

F

F

E

D

K

L

R

K

K

K

L

I

P

P

L

V

Y

Y

I

F

F

F

S

A

Q

Q

V

L

A

L

G

G

A

A

F

F

C

V

A

G

A

T

L

S

L

T

V

V

Y

Y

T

G

L

L

Y

Y

S

-

N

-

L

-

F

-

D

-

F

-

E

-

Q

-

T

-

H

H

Q

G

M

F

V

I

R

S

G

N

T

S

Q

K

A

I

S

P

L

-

E

-

L

-

A

Q

S

F

V

A

F

W

S

E

T

T

F
x
S

P

R

N

N

P
|
P

A
x
S

L

I

S

S

T

L

A

T

Q

G

A

A

F

F

L

F

V

N

E

E

V

L

I

I

I

L

T

T

A

G

I

I

L

L

M

L

G

L

V

V

I

I

M

L

S

V

L

V

T

V

D

D

D

E

N

N

N

I

G

-

L

C

P

G

K

K

G

F

P

H

L

I

A

L

P

K

L

L

-

S

L

V

I

V

G

G

L

L

L

I

I

I

A

L

V

C

I

I

G

G

S

I

S

T

M

F

G

G

P

G

L

N

T

T

G
|
G

F
|
F

A

A

M

L

N
|
N

P

P

A

S

R
|
R

D

D

F

L

G

G

P

S

K

R

L

F

M

L

T

S

F

L

F

I

A

A

G

-

W

Y

G

G

E

K

I

D

S

T

F

F

T

T

G

-

R

K

D

D

I

N

P

F

Y

Y

F

F

L

W

I

V

P

P

I

L

F

V

A

A

P

P

I

C

L

V

G

G

A

S

C

V

L

V

G

F

A

C

A

Q

A

F

Y

Y

R

D

G

K

L

V

I

I

A

C

R

-

H

-

L

-

P

P

I

L

A

V

I

D

P

L

A

A

T

N

K

N

D

E

A

K

E

D

G66 (= G67) binding glycerol
A67 (= A68) binding glycerol
N70 (= N71) binding glycerol
L71 (≠ P72) mutation to P: No significant effects on transport activity; when associated with A-131.
LA 71:72 (≠ PA 72:73) mutation to PS: Reduces stability and abolishes transport activity; when associated with 130-L-A-131.
S127 (≠ F141) binding glycerol
PS 130:131 (≠ PA 144:145) mutation to LA: Reduces stability and abolishes transport activity; when associated with 71-P-S-72.
S131 (≠ A145) mutation to A: No significant effects on transport activity; when associated with P-71.
G189 (= G202) binding glycerol
F190 (= F203) binding glycerol
N193 (= N206) binding glycerol
R196 (= R209) binding glycerol

3c02A X-ray structure of the aquaglyceroporin from plasmodium falciparum (see paper)
35% identity, 89% coverage: 9:259/283 of query aligns to 1:236/242 of 3c02A

query
sites
3c02A

S

S

L

Y

S

V

S

R

Q

E

C

F

M

I

A

G

E

E

F

F

L

L

G

G

T

T

A

F

L

V

L

L

I

M

F

F

F

L

G

G

T

E

G

G

C

A

V

T

A

A

A

N

L

F

K

H

V

T

A

T

G

G

A

L

S

S

F

G

G

D

L

W

W

Y

E

K

I

L

S

C

I

L

I

G

W
|
W

G

G

I

L

G

A

V

V

S

F

M

F

A

G

I

I

Y

L

L

V

T

S

A

A

G

K

V

L

S

S

G

G

A

A

H

H

L

L

N

N

P

L

A

A

V

V

S

S

I

I

A

G

L

L

C

S

I

S

F

I

A

N

D

K

F

F

E

D

K

L

R

K

K

K

L

I

P

P

L

V

Y

Y

I

F

F

F

S

A

Q

Q

V

L

A

L

G

G

A

A

F

F

C

V

A

G

A

T

L

S

L

T

V

V

Y

Y

T

G

L

L

Y

Y

S

-

N

-

L

-

F

-

D

-

F

-

E

-

Q

-

T

-

H

H

Q

G

M

F

V

I

R

S

G

N

T

S

Q

K

A

I

S

P

L

-

E

-

L

-

A

Q

S

F

V

A

F

W

S

E

T

T

F

S

P

R

N

N

P

P

A

S

L

I

S

S

T

L

A

T

Q

G

A

A

F

F

L

F

V

N

E

E

V

L

I

I

I

L

T

T

A

G

I

I

L

L

M

L

G

L

V

V

I

I

M

L

S

V

L

V

T

V

D

D

D

E

N

N

N

I

G

-

L

C

P

G

K

K

G

F

P

H

L

I

A

L

P

K

L

L

-

S

L

V

I

V

G

G

L

L

L

I

I

I

A

L

V

C

I

I

G

G

S

I

S

T

M

F

G

G

P

G

L

N

T

T

G
|
G

F
|
F

A

A

M

L

N

N

P

P

A

S

R
|
R

D

D

F

L

G

G

P

S

K

R

L

F

M

L

T

S

F

L

F

I

A

A

G

-

W

Y

G

G

E

K

I

D

S

T

F

F

T

T

G

-

R

K

D

D

I

N

P

F

Y

Y

F

F

L

W

I

V

P

P

I

L

F

V

A

A

P

P

I

C

L

V

G

G

A

S

C

V

L

V

G

F

A

C

A

Q

A

F

Y

Y

R

D

G

K

L

V

I

I

A

C

binding glycerol: W43 (= W51), G182 (= G202), F183 (= F203), R189 (= R209)

8ofxA Molecular mechanism of trypanosomal aqp2
33% identity, 85% coverage: 15:255/283 of query aligns to 7:239/244 of 8ofxA

query
sites
8ofxA

M

V

A

A

E

E

F

F

L

L

G

G

T

N

A

F

L

V

L

L

I

I

F

Y

F

I

G

A

T

K

G

G

C

A

V

V

A

I

A

T

L

S

K

L

V

L

A

V

G

-

A

P

S

D

F

F

G

G

L

L

W

L

E

G

I

L

S

T

I

I

I

G

W

I

G

G

I

V

G

A

V

V

S

T

M

M

A

A

I

L

Y

Y

L

V

T

S

A

L

G

G

V

I

S

S

G
|
G

A
x
G

H
|
H

L
|
L

N
|
N

P

S

A

A

V
|
V

S

T

I

V

A

G

L

N

C

A

I

V

F

F

A

G

D

D

F

F

E

P

K

W

R

R

K

K

L

V

P

P

L

G

Y

Y

I

I

F

A

S

A

Q

Q

V

M

A

L

G

G

A

T

F

F

C

L

A

G

A

A

L

A

L

C

V

A

Y

Y

T

G

L

V

Y

F

S

A

N

D

L

L

F

L

F

K

D

A

F

H

E

G

Q

G

T

G

H

E

Q

L

M

I

V

A

R

F

G

G

T

E

Q

K

A

G

S

-

L

-

E

-

L

I

A

A

S

W

V

V

F

F

S

A

T

M

F

Y

P

P

N

A

P

E

A

G

L

N

S

G

T

I

A

F

Q

Y

A

P

F

I

L

F

V

A

E

E

V

L

I

I

I

S

T

T

A

A

I

V

L
|
L

M

L

G

L

V

C

I
x
V

M

C

S

G

L

I

T
x
F

D

D

D

P

N

N

G

S

L

P

P

A

K

K

G

G

P

-

L

Y

A

E

P

T

L

V

L

A

I
|
I

G

G

L

A

L

L

I
x
V

A

F

V

V

I

M

G

V

S

N

M

F

G

G

P

L

L

A

T

S

G

P

F

L

A
|
A

M
|
M

N
|
N

P

P

A

S

R
x
L

D

D

F

F

G

G

P

P

K

R

L

V

M

F

T

G

F

A

F

I

A

L

G

L

W

G

G

G

E

E

I

V

S

-

F

F

T

S

G

H

G

A

R

N

D

Y

I

-

P

-

Y

Y

F

F

L

W

I

V

P

P

I

L

F

V

A

V

P

P

I

F

L

F

G

G

A

A

C

I

L

L

G

G

A

L

A

F

A

L

Y

Y

R

K

binding [(2~{R},4~{S})-2-[4-[[4,6-bis(azanyl)-1,3,5-triazin-2-yl]amino]phenyl]-1,3,2-dithiarsolan-4-yl]methanol: G58 (= G67), G59 (≠ A68), H60 (= H69), L61 (= L70), N62 (= N71), V65 (= V74), L150 (= L162), V154 (≠ I166), F158 (≠ T170), I173 (= I186), V177 (≠ I190), A191 (= A204), M192 (= M205), N193 (= N206), L196 (≠ R209)

8jy8A Structure of tbaqp2 in complex with anti-trypanosomatid drug pentamidine (see paper)
33% identity, 85% coverage: 15:255/283 of query aligns to 5:237/242 of 8jy8A

query
sites
8jy8A

M

V

A

A

E

E

F

F

L

L

G

G

T

N

A

F

L

V

L

L

I

I

F

Y

F

I

G

A

T

K

G

G

C

A

V

V

A

I

A

T

L

S

K

L

V

L

A

V

G

-

A

P

S

D

F

F

G

G

L

L

W

L

E

G

I

L

S

T

I

I

I

G

W

I

G

G

I

V

G

A

V

V

S

T

M

M

A

A

I

L

Y

Y

L

V

T

S

A

L

G

G

V

I

S

S

G

G

A

G

H

H

L

L

N

N

P

S

A

A

V

V

S

T

I

V

A

G

L

N

C

A

I

V

F

F

A

G

D

D

F

F

E

P

K

W

R

R

K

K

L

V

P

P

L

G

Y

Y

I

I

F

A

S

A

Q

Q

V

M

A

L

G

G

A

T

F

F

C

L

A

G

A

A

L

A

L

C

V

A

Y

Y

T

G

L

V

Y

F

S

A

N

D

L

L

F

L

F

K

D

A

F

H

E

G

Q

G

T

G

H

E

Q

L

M

I

V

A

R

F

G

G

T

E

Q

K

A

G

S

-

L

-

E

-

L

I

A

A

S

W

V

V

F

F

S

A

T

M

F

Y

P

P

N

A

P

E

A

G

L

N

S

G

T

I

A

F

Q

Y

A

P

F

I

L

F

V

A

E

E

V

L

I

I

I

S

T

T

A

A

I

V

L

L

M

L

G

L

V

C

I

V

M

C

S

G

L

I

T

F

D

D

D

P

N

N

G

S

L

P

P

A

K

K

G

G

P

-

L

Y

A

E

P

T

L

V

L

A

I

I

G

G

L

A

L

L

I
x
V

A

F

V

V

I

M

G

V

S

N

M

F

G

G

P

L

L

A

T

S

G

P

F

L

A
|
A

M

M

N
|
N

P

P

A

S

R
x
L

D

D

F

F

G

G

P

P

K

R

L

V

M

F

T

G

F

A

F

I

A

L

G

L

W

G

G

G

E

E

I

V

S

-

F

F

T

S

G

H

G

A

R

N

D

Y

I

-

P

-

Y

Y

F

F

L

W

I

V

P

P

I

L

F

V

A

V

P

P

I

F

L

F

G

G

A

A

C

I

L

L

G

G

A

L

A

F

A

L

Y

Y

R

K

binding 1,5-bis(4-amidinophenoxy)pentane: V175 (≠ I190), A189 (= A204), N191 (= N206), L194 (≠ R209)

8jy6A Structure of tbaqp2 in complex with anti-trypanosomatid drug melarsoprol (see paper)
33% identity, 85% coverage: 15:255/283 of query aligns to 5:237/242 of 8jy6A

query
sites
8jy6A

M

V

A

A

E

E

F

F

L

L

G

G

T

N

A

F

L

V

L

L

I

I

F

Y

F

I

G

A

T

K

G

G

C

A

V

V

A

I

A

T

L

S

K

L

V

L

A

V

G

-

A

P

S

D

F

F

G

G

L

L

W

L

E

G

I

L

S

T

I

I

I

G

W

I

G

G

I

V

G

A

V

V

S

T

M

M

A

A

I

L

Y

Y

L

V

T

S

A

L

G

G

V

I

S

S

G

G

A

G

H
|
H

L
|
L

N

N

P

S

A

A

V

V

S

T

I

V

A

G

L

N

C

A

I

V

F

F

A

G

D

D

F

F

E

P

K

W

R

R

K

K

L

V

P

P

L

G

Y

Y

I

I

F

A

S

A

Q

Q

V

M

A

L

G

G

A

T

F

F

C

L

A

G

A

A

L

A

L

C

V

A

Y

Y

T

G

L

V

Y

F

S

A

N

D

L

L

F

L

F

K

D

A

F

H

E

G

Q

G

T

G

H

E

Q

L

M

I

V

A

R

F

G

G

T

E

Q

K

A

G

S

-

L

-

E

-

L

I

A

A

S

W

V

V

F

F

S

A

T
x
M

F

Y

P

P

N

A

P

E

A

G

L

N

S

G

T

I

A

F

Q

Y

A

P

F

I

L

F

V

A

E

E

V

L

I

I

I

S

T

T

A

A

I

V

L

L

M

L

G

L

V

C

I

V

M

C

S

G

L

I

T

F

D

D

D

P

N

N

G

S

L

P

P

A

K

K

G

G

P

-

L

Y

A

E

P

T

L

V

L

A

I
|
I

G

G

L

A

L

L

I
x
V

A

F

V

V

I

M

G
x
V

S

N

M

F

G

G

P

L

L

A

T

S

G

P

F

L

A
|
A

M

M

N
|
N

P

P

A

S

R
x
L

D

D

F

F

G

G

P

P

K

R

L

V

M

F

T

G

F

A

F

I

A

L

G

L

W

G

G

G

E

E

I

V

S

-

F

F

T

S

G

H

G

A

R

N

D

Y

I

-

P

-

Y

Y

F

F

L

W

I

V

P

P

I

L

F

V

A

V

P

P

I

F

L

F

G

G

A

A

C

I

L

L

G

G

A

L

A

F

A

L

Y

Y

R

K

binding [(4~{R})-2-[4-[[4,6-bis(azanyl)-1,3,5-triazin-2-yl]amino]phenyl]-1,3,2-dithiarsolan-4-yl]methanol: H58 (= H69), L59 (= L70), M126 (≠ T140), I171 (= I186), V175 (≠ I190), V179 (≠ G194), A189 (= A204), N191 (= N206), L194 (≠ R209)

2evuA Crystal structure of aquaporin aqpm at 2.3a resolution (see paper)
34% identity, 88% coverage: 9:257/283 of query aligns to 3:243/245 of 2evuA

query
sites
2evuA

S

S

L

L

S

T

S

K

Q

R

C

C

M

I

A

A

E

E

F

F

L

I

G

G

T

T

A

F

L

I

L

L

I

V

F

F

G

G

T

A

G

G

C

S

V

-

A

A

L

V

K

T

V

L

A

M

G

I

A

A

S

S

F

G

G

G

L

T

-

S

-

P

-

N

-

P

-

F

-

N

-
x
I

-

G

-

I

-

G

-

L

-

G

-

L

-

G

-

D

W
|
W

-

V

E

A

I

I

S

G

I

L

I

A

W

F

G

G

I

F

G

A

V
x
I

S

A

M

A

A

S

I

I

Y

Y

L

A

T

L

A

G

G

N

V

I

S

S

G

G

A

C

H

H

L
x
I

N
|
N

P

P

A

A

V

V

S

T

I

I

A

G

L

L

C

W

I

S

F

V

A

K

D

K

F

F

E

P

K

G

R

R

K

E

L

V

P

V

L

P

Y

Y

I

I

F

I

S

A

Q

Q

V

L

A

L

G

G

A

A

F

A

C

F

A

G

A

S

L

-

V

-

Y

-

T

-

L

-

Y

-

S

-

N

-

L

-

F

-

F

F

D

I

F

F

E

L

Q

Q

T

C

H

A

Q

G

M

I

V

G

R

A

G

A

T

T

Q

V

A

G

S

G

L

L

E
x
G

L

-

A

-

S

-

V

-

F

-

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x
A

T

T

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x
A

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|
P

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x
F

P

P

A

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Y

A

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Q

Q

A

A

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V

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A

F

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M

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T

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D

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N

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N

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A

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G

G

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-

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F

A

A

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G

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I

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A

A

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M

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N

L

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N

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A

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P

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-

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-

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-

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-

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P

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A

A

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A

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G

Y

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L

binding glycerol: I44 (vs. gap), W55 (= W45), I66 (≠ V55), I81 (≠ L70), N82 (= N71), G133 (≠ E133), A134 (≠ S139), A136 (≠ F141), P137 (= P142), F138 (≠ N143)

P08995 Nodulin-26; N-26 from Glycine max (Soybean) (Glycine hispida) (see paper)
31% identity, 95% coverage: 12:280/283 of query aligns to 38:268/271 of P08995

query
sites
P08995

S

Q

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A

S262 (≠ P274) modified: Phosphoserine; by CPK

Q9SAI4 Aquaporin NIP6-1; NOD26-like intrinsic protein 6-1; AtNIP6;1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
32% identity, 87% coverage: 9:254/283 of query aligns to 77:285/305 of Q9SAI4

query
sites
Q9SAI4

S

S

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x
A

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x
V

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Y

A119 (≠ W51) mutation to W: 6-fold increase in water transport activity, but impaired in urea transport.
V252 (≠ A208) mutation to A: No effect.

P41181 Aquaporin-2; AQP-2; ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct from Homo sapiens (Human) (see 12 papers)
29% identity, 96% coverage: 9:281/283 of query aligns to 8:249/271 of P41181

query
sites
P41181

S

A

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F

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Q

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|
G

A

A

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x
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R

R

G64 (= G67) to R: in NDI2; loss of water channel activity; dbSNP:rs104894326
G78 (≠ F81) mutation to A: Does not affect interaction with MIAC; when associated with A-79.
C79 (≠ A82) mutation to A: Does not affect interaction with MIAC; when associated with A-78.
S148 (≠ L169) mutation to A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; mutation to D: Retained in the endoplasmic reticulum.
R187 (= R209) to C: in NDI2; loss of water channel activity; mutant protein does not fold properly; dbSNP:rs104894328
A190 (≠ G212) to T: in NDI2; mutant protein does not fold properly and is not functional; dbSNP:rs104894341
V194 (≠ M216) to I: in dbSNP:rs772051028
S216 (≠ A251) to P: in NDI2; loss of water channel activity; dbSNP:rs104894329
L217 (≠ A252) mutation to A: Abolishes interaction with MIAC; when associated with A-221.
Y221 (≠ G256) mutation to A: Abolishes interaction with MIAC; when associated with A-217.
S229 (≠ H261) mutation to A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; mutation to D: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.
S231 (≠ P263) mutation to A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; mutation to D: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.
E232 (≠ I264) mutation to A: Reduces interaction with MIAC.
T244 (≠ I276) mutation to A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; mutation to E: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.

Sites not aligning to the query:

254 R → L: in NDI2; results in the loss of arginine vasopressin-mediated phosphorylation at S-256; R → Q: in NDI2; exerts a dominant-negative effect on wild-type-AQP2 in that it interferes with its trafficking to the apical membrane; is a loss of function instead of a gain of function mutation on dominant nephrogenic diabetes insipidus
256 modified: Phosphoserine; by PKA; S→A: Retained in vesicles.; S→D: Expressed in the apical membrane.
258 E → K: in NDI2; retained in the Golgi compartment; dbSNP:rs104894332
262 P → L: in NDI2; mutant protein folds properly and is functional but is retained in intracellular vesicles; able to assemble into tetramers with wild-type AQP2 that properly localize to the apical membrane; dbSNP:rs104894339; P→A: No effect on expression at the apical cell membrane.

Q6Z2T3 Aquaporin NIP2-1; Low silicon protein 1; NOD26-like intrinsic protein 2-1; OsNIP2;1; Silicon influx transporter LSI1 from Oryza sativa subsp. japonica (Rice) (see paper)
28% identity, 87% coverage: 8:254/283 of query aligns to 45:254/298 of Q6Z2T3

query
sites
Q6Z2T3

P

P

S

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P

-

I

-

F

-

A

G

P

P

I

V

L

M

G

G

A

T

C

L

L

S

G

G

A

A

A

W

A

T

Y

Y

A132 (≠ S95) mutation to T: In lsi; impairs silicon uptake. Grain discoloration. Reduces grain yield 10-fold.

Query Sequence

>AO353_04175 FitnessBrowser__pseudo3_N2E3:AO353_04175
MTTASQQPSLSSQCMAEFLGTALLIFFGTGCVAALKVAGASFGLWEISIIWGIGVSMAIY
LTAGVSGAHLNPAVSIALCIFADFEKRKLPLYIFSQVAGAFCAALLVYTLYSNLFFDFEQ
THQMVRGTQASLELASVFSTFPNPALSTAQAFLVEVIITAILMGVIMSLTDDNNGLPKGP
LAPLLIGLLIAVIGSSMGPLTGFAMNPARDFGPKLMTFFAGWGEISFTGGRDIPYFLIPI
FAPILGACLGAAAYRGLIARHLPIAIPATKDAEPAIDGKARIS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory